Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0014881

Physical Location & Seq

Genomic Coordinates Reverse (-)

5934388 .. 5936572

Gene Structure 2185 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G006250.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1569 bp

ATGCTTATGCAAACTTGCACCCAAATTGAAAACCAAAGCTCATGCCTCACAGAAGTGCAAGCTGAGCTCCAAAGCATAATGGGACCTGATCAAAGTCATCAAAACGCTGCTTCTGCAAGAAGTTCAATCCTAAGTGCTGCGCTTAGGCACACACTTAATCAAGCGAAAGCTGCAGTTGACATGATCACAAAGTTCAATGCTCTATCCATCGGTTACAGAGAACAAGTAGCTATTGAGGATTGCAAGGAGCTTCTTGATTTCTCTGTCTCTGAGTTGGCTTGGTCTTTAGGCGAGATGAACAGAATTCGGACAGGTGACAAAAATGTTCATGAAGGAGATCTAAAAGCTTGGCTGAGTGCTGCCCTTAGTAACCAAGATACATGCCTTGAAGGTTTCGAGGGAACTGATAGACGGCTCGAAAATTTCATAAGGGGAAGCTTGAAGCAAGTCACACAGCTCATTGGTAATGTCTTGGCCTTGTATACTCAATTACATGCTTTACCCTTTAAACCTCCAAGGGATCATAGTATGAATACTTTGACAATTGCGAATAAGAGTTCATCGCCGGATCATAATTTTCCGGCATGGATGAGTGACGGTGATCAAGAGCTGGTTAAGTCAAGTCCACATGATCAATCAGGTATGCATGTAGATGCTGTTGTGGCAACAGATGGGAGCGGGAAGTACCGTTCAATCACAGAAGCTGTTAATGAAGCTCCAGACCACAGCAGCAGGAGGTACATAATATATGTGAAGAAGGGGATTTATAGAGAAAACATTGACATGAAGAAGAAGAAAACCAATATCATGTTTGTAGGGGATGGAATTGGACAAACTGTGGTAACTGGTGACCGAAGTTTCATGCAAGGATGGACTACATTTAGAACTGCAACAGTTGCTGTATCCGGAAAAGGATTTATAGCAAGAGACATGACTTTTCGTAACACGGCCGGGCCTGAAAACCATCAAGGCGTGGCGCTTAGGGTTGACTCGGACCAATCTGCCTTCTTCCGGTGCAGCATGGAGGGTCACCAAGACACACTGTATGCTCACTCTCTTCGCCAATTTTATCGTGAATGTGACATCTATGGTACCATAGACTTCATATTTGGCAATGGTGCCGCGGTGCTCCAAAATTGCAAAATCTACACTCGAGTCCCGCTATCACTACAAAAGGTCACCATCACGGCCCAGGGTCGGAAAGATCCGCATCAAAACACTGGATTTTCGATCCAAGATAGCTATGTTTTTGCTACTCAACCGACATATCTGGGGAGGCCTTGGAAGCCATACTCTAGGACAGTTTTTATGAACACTTACTTGAGTGGTCTAGTGCAGCCTAGAGGGTGGCTTGAGTGGTATGGTAACTTTGCTTTGGGCACATTATGGTACGGCGAGTATAGAAACTATGGGCCGGGTGCATCACTATCCGGACGGGTCAAGTGGCCAGGTTACCACATCATTCAGGATGCCAACATGGCTAGTTTCTTCACGGTTCGGCGATTTATTGATGGGATGGCTTGGTTGCCGGCCACAGGTGTTAAGTTTACAGTAGGTTTGAGAAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

522

Amino Acids

58.4

Weight (kDa)

8.52

Isoelectric Point (pI)

37.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	4 - 160	2.2e-26	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	218 - 505	5.4e-140	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014881)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05620
fragaria_vesca	FvH4_6g06871
malus_domestica	MD04G1198000.v1.1 MD12G1210600.v1.1
prunus_persica	Prupe.6G318500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0456351
rosa_laevigata	RLG00000025310
rosa_multiflora	Rmu_sc0002607.1_g000020 Rmu_sc0018054.1_g000002
rosa_roxburghii	Rroxscaffold_6G00422790
rosa_rugosa	Rorug03G0012700
rosa_samantha	Rh3AG072800 Rh3BG074900 Rh3CG074500 Rh3DG076300
rosa_wichuraiana	Rw3G006250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1091
AccB1I	GGYRCC	2 cut(s)	1091, 1118
AccBSI	CCGCTC	1 cut(s)	678
AccI	GTMKAC	1 cut(s)	482
AccII	CGCG	1 cut(s)	1124
AccIII	TCCGGA	2 cut(s)	905, 1430
AciI	CCGC	5 cut(s)	678, 1122, 1124, 1160, 1208
AclWI	GGATC	4 cut(s)	528, 576, 1199, 1225
AcoI	YGGCCR	3 cut(s)	948, 1445, 1530
AcsI	RAATTY	2 cut(s)	303, 421
AfaI	GTAC	4 cut(s)	686, 740, 1093, 1391
AfiI	CCNNNNNNNGG	3 cut(s)	1011, 1197, 1535
AgsI	TTSAA	6 cut(s)	29, 126, 196, 389, 442, 693
AjnI	CCWGG	2 cut(s)	1191, 1447
AleI	CACNNNNGTG	1 cut(s)	53
AloI	GAACNNNNNNTCC	2 cut(s)	673, 705
Alw21I	GWGCWC	2 cut(s)	69, 1131
Alw26I	GTCTC	2 cut(s)	271, 921
AlwI	GGATC	4 cut(s)	528, 576, 1199, 1225
AlwNI	CAGNNNCTG	2 cut(s)	704, 899
Ama87I	CYCGRG	1 cut(s)	1152
Aor13HI	TCCGGA	2 cut(s)	905, 1430
AoxI	GGCC	8 cut(s)	474, 948, 953, 1188, 1277, 1412, 1445, 1530
ApeKI	GCWGC	7 cut(s)	107, 137, 170, 359, 729, 1017, 1336
ApoI	RAATTY	2 cut(s)	303, 421
ArsI	GACNNNNNNTTYG	2 cut(s)	1091, 1123
Asp718I	GGTACC	1 cut(s)	1091
AspLEI	GCGC	2 cut(s)	142, 979
AspS9I	GGNCC	5 cut(s)	83, 953, 994, 1189, 1412
AsuC2I	CCSGG	2 cut(s)	952, 1416
AsuHPI	GGTGA	5 cut(s)	326, 611, 860, 1022, 1171
AvaI	CYCGRG	1 cut(s)	1152
AvaII	GGWCC	2 cut(s)	83, 994
BaeGI	GKGCMC	1 cut(s)	1382
BalI	TGGCCA	1 cut(s)	1447
BanI	GGYRCC	2 cut(s)	1091, 1118
BanII	GRGCYC	1 cut(s)	69
Bbv12I	GWGCWC	2 cut(s)	69, 1131
BbvI	GCAGC	7 cut(s)	94, 124, 157, 346, 741, 1029, 1348
BccI	CCATC	8 cut(s)	215, 665, 815, 864, 972, 1190, 1505, 1510
BceAI	ACGGC	4 cut(s)	428, 963, 1203, 1408
BciT130I	CCWGG	2 cut(s)	1193, 1449
BciVI	GTATCC	1 cut(s)	913
BclI	TGATCA	4 cut(s)	88, 183, 601, 631
BcnI	CCSGG	2 cut(s)	952, 1416
BcoDI	GTCTC	2 cut(s)	271, 921
BfaI	CTAG	4 cut(s)	1296, 1331, 1341, 1482
BfmI	CTRYAG	1 cut(s)	171
BfoI	RGCGCY	1 cut(s)	980
BfuI	GTATCC	1 cut(s)	913
BglI	GCCNNNNNGGC	1 cut(s)	1478
BglII	AGATCT	1 cut(s)	337
BisI	GCNGC	8 cut(s)	108, 138, 171, 360, 730, 1018, 1122, 1337
BlpI	GCTNAGC	1 cut(s)	63
BlsI	GCNGC	8 cut(s)	109, 139, 172, 361, 731, 1019, 1123, 1338
Bme1390I	CCNGG	4 cut(s)	952, 1193, 1416, 1449
Bme18I	GGWCC	2 cut(s)	83, 994
BmeT110I	CYCGRG	1 cut(s)	1152
BmgT120I	GGNCC	5 cut(s)	83, 953, 994, 1189, 1412
BmiI	GGNNCC	3 cut(s)	84, 1093, 1120
BmrFI	CCNGG	4 cut(s)	952, 1193, 1416, 1449
BmsI	GCATC	4 cut(s)	643, 1219, 1430, 1459
BpmI	CTGGAG	1 cut(s)	702
Bpu10I	CCTNAGC	2 cut(s)	143, 980
Bpu1102I	GCTNAGC	1 cut(s)	63
BpuEI	CTTGAG	2 cut(s)	1342, 1373
BpuMI	CCSGG	2 cut(s)	952, 1416
BsaBI	GATNNNNATC	1 cut(s)	1209
BsaJI	CCNNGG	5 cut(s)	515, 1122, 1191, 1192, 1280
BsaWI	WCCGGW	3 cut(s)	905, 1011, 1430
Bsc4I	CCNNNNNNNGG	3 cut(s)	1011, 1197, 1535
Bse118I	RCCGGY	1 cut(s)	1528
Bse1I	ACTGG	2 cut(s)	850, 1225
Bse3DI	GCAATG	1 cut(s)	1120
Bse8I	GATNNNNATC	1 cut(s)	1209
BseAI	TCCGGA	2 cut(s)	905, 1430
BseBI	CCWGG	2 cut(s)	1193, 1449
BseDI	CCNNGG	5 cut(s)	515, 1122, 1191, 1192, 1280
BseGI	GGATG	5 cut(s)	594, 826, 875, 1474, 1521
BseJI	GATNNNNATC	1 cut(s)	1209
BseLI	CCNNNNNNNGG	3 cut(s)	1011, 1197, 1535
BseMI	GCAATG	1 cut(s)	1120
BseMII	CTCAG	3 cut(s)	54, 261, 344
BseNI	ACTGG	2 cut(s)	850, 1225
BseSI	GKGCMC	1 cut(s)	1382
BseX3I	CGGCCG	1 cut(s)	948
BseXI	GCAGC	7 cut(s)	94, 124, 157, 346, 741, 1029, 1348
BsgI	GTGCAG	2 cut(s)	1036, 1355
Bsh1236I	CGCG	1 cut(s)	1124
Bsh1285I	CGRYCG	1 cut(s)	951
BshFI	GGCC	8 cut(s)	476, 950, 955, 1190, 1279, 1414, 1447, 1532
BshNI	GGYRCC	2 cut(s)	1091, 1118
BsiEI	CGRYCG	1 cut(s)	951
BsiHKAI	GWGCWC	2 cut(s)	69, 1131
BsiHKCI	CYCGRG	1 cut(s)	1152
BsiSI	CCGG	8 cut(s)	566, 581, 906, 951, 1012, 1415, 1431, 1529
BslFI	GGGAC	2 cut(s)	96, 1142
BslI	CCNNNNNNNGG	3 cut(s)	1011, 1197, 1535
BsmAI	GTCTC	2 cut(s)	271, 921
BsmFI	GGGAC	2 cut(s)	96, 1142
BsnI	GGCC	8 cut(s)	476, 950, 955, 1190, 1279, 1414, 1447, 1532
BsoBI	CYCGRG	1 cut(s)	1152
Bsp1286I	GDGCHC	3 cut(s)	69, 1131, 1382
Bsp13I	TCCGGA	2 cut(s)	905, 1430
Bsp143I	GATC	9 cut(s)	88, 183, 337, 520, 568, 601, 631, 1204, 1230
Bsp1720I	GCTNAGC	1 cut(s)	63
BspACI	CCGC	5 cut(s)	678, 1122, 1124, 1160, 1208
BspANI	GGCC	8 cut(s)	476, 950, 955, 1190, 1279, 1414, 1447, 1532
BspCNI	CTCAG	3 cut(s)	55, 262, 345
BspEI	TCCGGA	2 cut(s)	905, 1430
BspFNI	CGCG	1 cut(s)	1124
BspHI	TCATGA	1 cut(s)	328
BspLI	GGNNCC	3 cut(s)	84, 1093, 1120
BspMAI	CTGCAG	1 cut(s)	175
BspPI	GGATC	4 cut(s)	528, 576, 1199, 1225
BspT107I	GGYRCC	2 cut(s)	1091, 1118
BsrBI	CCGCTC	1 cut(s)	678
BsrDI	GCAATG	1 cut(s)	1120
BsrFI	RCCGGY	1 cut(s)	1528
BsrI	ACTGG	2 cut(s)	850, 1225
BssAI	RCCGGY	1 cut(s)	1528
BssECI	CCNNGG	5 cut(s)	515, 1122, 1191, 1192, 1280
BssMI	GATC	9 cut(s)	88, 183, 337, 520, 568, 601, 631, 1204, 1230
BssNAI	GTATAC	1 cut(s)	483
BssT1I	CCWWGG	2 cut(s)	515, 1280
Bst1107I	GTATAC	1 cut(s)	483
Bst2UI	CCWGG	2 cut(s)	1193, 1449
Bst4CI	ACNGT	8 cut(s)	599, 689, 838, 895, 1044, 1303, 1495, 1552
Bst6I	CTCTTC	1 cut(s)	1062
BstAPI	GCANNNNNTGC	1 cut(s)	896
BstC8I	GCNNGC	2 cut(s)	60, 1530
BstDEI	CTNAG	7 cut(s)	63, 131, 143, 270, 353, 365, 980
BstDSI	CCRYGG	1 cut(s)	1122
BstEII	GGTNACC	4 cut(s)	848, 1028, 1177, 1451
BstF5I	GGATG	5 cut(s)	594, 826, 875, 1474, 1521
BstFNI	CGCG	1 cut(s)	1124
BstH2I	RGCGCY	1 cut(s)	980
BstHHI	GCGC	2 cut(s)	142, 979
BstKTI	GATC	9 cut(s)	91, 186, 340, 523, 571, 604, 634, 1207, 1233
BstMAI	GTCTC	2 cut(s)	271, 921
BstMBI	GATC	9 cut(s)	88, 183, 337, 520, 568, 601, 631, 1204, 1230
BstMCI	CGRYCG	1 cut(s)	951
BstMWI	GCNNNNNNNGC	7 cut(s)	64, 113, 170, 662, 896, 1285, 1478
BstNI	CCWGG	2 cut(s)	1193, 1449
BstNSI	RCATGY	3 cut(s)	384, 497, 650
BstPI	GGTNACC	4 cut(s)	848, 1028, 1177, 1451
BstSCI	CCNGG	4 cut(s)	950, 1191, 1414, 1447
BstSFI	CTRYAG	1 cut(s)	171
BstSLI	GKGCMC	1 cut(s)	1382
BstUI	CGCG	1 cut(s)	1124
BstV1I	GCAGC	7 cut(s)	94, 124, 157, 346, 741, 1029, 1348
BstX2I	RGATCY	2 cut(s)	337, 1204
BstYI	RGATCY	2 cut(s)	337, 1204
BstZ17I	GTATAC	1 cut(s)	483
BstZI	CGGCCG	1 cut(s)	948
BsuI	GTATCC	1 cut(s)	913
BsuRI	GGCC	8 cut(s)	476, 950, 955, 1190, 1279, 1414, 1447, 1532
BtgI	CCRYGG	1 cut(s)	1122
BtgZI	GCGATG	1 cut(s)	546
BtsCI	GGATG	5 cut(s)	594, 826, 875, 1474, 1521
BtsIMutI	CAGTG	2 cut(s)	1040, 1218
Cac8I	GCNNGC	2 cut(s)	60, 1530
CaiI	CAGNNNCTG	2 cut(s)	704, 899
CciI	TCATGA	1 cut(s)	328
CfoI	GCGC	2 cut(s)	142, 979
Cfr10I	RCCGGY	1 cut(s)	1528
Cfr13I	GGNCC	5 cut(s)	83, 953, 994, 1189, 1412
Cfr42I	CCGCGG	1 cut(s)	1125
Csp6I	GTAC	4 cut(s)	685, 739, 1092, 1390
CviQI	GTAC	4 cut(s)	685, 739, 1092, 1390
DdeI	CTNAG	7 cut(s)	63, 131, 143, 270, 353, 365, 980
DpnI	GATC	9 cut(s)	90, 185, 339, 522, 570, 603, 633, 1206, 1232
DpnII	GATC	9 cut(s)	88, 183, 337, 520, 568, 601, 631, 1204, 1230
DraI	TTTAAA	1 cut(s)	508
EaeI	YGGCCR	3 cut(s)	948, 1445, 1530
EagI	CGGCCG	1 cut(s)	948
Eam1104I	CTCTTC	1 cut(s)	1062
EarI	CTCTTC	1 cut(s)	1062
Ecl136II	GAGCTC	1 cut(s)	67
EclXI	CGGCCG	1 cut(s)	948
Eco130I	CCWWGG	2 cut(s)	515, 1280
Eco147I	AGGCCT	1 cut(s)	1279
Eco24I	GRGCYC	1 cut(s)	69
Eco47I	GGWCC	2 cut(s)	83, 994
Eco52I	CGGCCG	1 cut(s)	948
Eco53kI	GAGCTC	1 cut(s)	67
Eco88I	CYCGRG	1 cut(s)	1152
Eco91I	GGTNACC	4 cut(s)	848, 1028, 1177, 1451
EcoICRI	GAGCTC	1 cut(s)	67
EcoO109I	RGGNCCY	1 cut(s)	83
EcoO65I	GGTNACC	4 cut(s)	848, 1028, 1177, 1451
EcoRI	GAATTC	1 cut(s)	303
EcoRII	CCWGG	2 cut(s)	1191, 1447
EcoT14I	CCWWGG	2 cut(s)	515, 1280
EcoT22I	ATGCAT	1 cut(s)	648
EcoT38I	GRGCYC	1 cut(s)	69
ErhI	CCWWGG	2 cut(s)	515, 1280
FaqI	GGGAC	2 cut(s)	96, 1142
FauI	CCCGC	2 cut(s)	671, 1167
FbaI	TGATCA	4 cut(s)	88, 183, 601, 631
FblI	GTMKAC	1 cut(s)	482
Fnu4HI	GCNGC	8 cut(s)	108, 138, 171, 360, 730, 1018, 1122, 1337
FokI	GGATG	5 cut(s)	601, 833, 882, 1481, 1528
FriOI	GRGCYC	1 cut(s)	69
Fsp4HI	GCNGC	8 cut(s)	108, 138, 171, 360, 730, 1018, 1122, 1337
FspBI	CTAG	4 cut(s)	1296, 1331, 1341, 1482
GlaI	GCGC	2 cut(s)	141, 978
GluI	GCNGC	8 cut(s)	108, 138, 171, 360, 730, 1018, 1122, 1337
GsuI	CTGGAG	1 cut(s)	702
HaeII	RGCGCY	1 cut(s)	980
HaeIII	GGCC	8 cut(s)	476, 950, 955, 1190, 1279, 1414, 1447, 1532
HapII	CCGG	8 cut(s)	566, 581, 906, 951, 1012, 1415, 1431, 1529
HhaI	GCGC	2 cut(s)	142, 979
Hin6I	GCGC	2 cut(s)	140, 977
HinP1I	GCGC	2 cut(s)	140, 977
HincII	GTYRAC	2 cut(s)	178, 988
HindII	GTYRAC	2 cut(s)	178, 988
HindIII	AAGCTT	2 cut(s)	345, 436
HinfI	GANTC	2 cut(s)	989, 1155
HpaII	CCGG	8 cut(s)	566, 581, 906, 951, 1012, 1415, 1431, 1529
HphI	GGTGA	5 cut(s)	326, 611, 860, 1022, 1171
Hpy166II	GTNNAC	5 cut(s)	178, 483, 626, 988, 1548
Hpy188I	TCNGA	4 cut(s)	271, 309, 994, 1200
Hpy188III	TCNNGA	8 cut(s)	254, 329, 605, 719, 906, 1073, 1431, 1466
Hpy8I	GTNNAC	5 cut(s)	178, 483, 626, 988, 1548
HpyAV	CCTTC	4 cut(s)	326, 383, 751, 1015
HpyCH4III	ACNGT	8 cut(s)	599, 689, 838, 895, 1044, 1303, 1495, 1552
HpyF10VI	GCNNNNNNNGC	7 cut(s)	64, 113, 170, 662, 896, 1285, 1478
HpyF3I	CTNAG	7 cut(s)	63, 131, 143, 270, 353, 365, 980
HspAI	GCGC	2 cut(s)	140, 977
Kpn2I	TCCGGA	2 cut(s)	905, 1430
KpnI	GGTACC	1 cut(s)	1095
KroI	GCCGGC	1 cut(s)	1528
KroNI	GCCGGC	1 cut(s)	1530
Ksp22I	TGATCA	4 cut(s)	88, 183, 601, 631
KspI	CCGCGG	1 cut(s)	1125
Kzo9I	GATC	9 cut(s)	88, 183, 337, 520, 568, 601, 631, 1204, 1230
LmnI	GCTCC	5 cut(s)	72, 247, 675, 721, 1134
Lsp1109I	GCAGC	7 cut(s)	94, 124, 157, 346, 741, 1029, 1348
LweI	GCATC	4 cut(s)	643, 1219, 1430, 1459
MaeI	CTAG	4 cut(s)	1296, 1331, 1341, 1482
MalI	GATC	9 cut(s)	90, 185, 339, 522, 570, 603, 633, 1206, 1232
MbiI	CCGCTC	1 cut(s)	678
MboI	GATC	9 cut(s)	88, 183, 337, 520, 568, 601, 631, 1204, 1230
MboII	GAAGA	7 cut(s)	766, 799, 802, 805, 1000, 1049, 1480
MfeI	CAATTG	1 cut(s)	543
MflI	RGATCY	2 cut(s)	337, 1204
MhlI	GDGCHC	3 cut(s)	69, 1131, 1382
MlsI	TGGCCA	1 cut(s)	1447
MluCI	AATT	9 cut(s)	24, 303, 421, 488, 543, 574, 825, 1064, 1135
MluNI	TGGCCA	1 cut(s)	1447
MlyI	GAGTC	2 cut(s)	983, 1164
MmeI	TCCRAC	1 cut(s)	1178
MnlI	CCTC	8 cut(s)	56, 229, 391, 522, 729, 1018, 1269, 1337
Mox20I	TGGCCA	1 cut(s)	1447
Mph1103I	ATGCAT	1 cut(s)	648
MroI	TCCGGA	2 cut(s)	905, 1430
MroNI	GCCGGC	1 cut(s)	1528
MscI	TGGCCA	1 cut(s)	1447
MseI	TTAA	5 cut(s)	156, 507, 615, 708, 1542
MslI	CAYNNNNRTG	3 cut(s)	53, 651, 782
Msp20I	TGGCCA	1 cut(s)	1447
MspA1I	CMGCKG	1 cut(s)	1124
MspI	CCGG	8 cut(s)	566, 581, 906, 951, 1012, 1415, 1431, 1529
MspR9I	CCNGG	4 cut(s)	952, 1193, 1416, 1449
MunI	CAATTG	1 cut(s)	543
MvaI	CCWGG	2 cut(s)	1193, 1449
MvnI	CGCG	1 cut(s)	1124
MwoI	GCNNNNNNNGC	7 cut(s)	64, 113, 170, 662, 896, 1285, 1478
NaeI	GCCGGC	1 cut(s)	1530
NciI	CCSGG	2 cut(s)	952, 1416
NdeII	GATC	9 cut(s)	88, 183, 337, 520, 568, 601, 631, 1204, 1230
NgoMIV	GCCGGC	1 cut(s)	1528
NlaIV	GGNNCC	3 cut(s)	84, 1093, 1120
NmuCI	GTSAC	7 cut(s)	314, 448, 593, 848, 1028, 1079, 1177
NsiI	ATGCAT	1 cut(s)	648
NspI	RCATGY	3 cut(s)	384, 497, 650
OliI	CACNNNNGTG	1 cut(s)	53
PaeR7I	CTCGAG	1 cut(s)	1152
PagI	TCATGA	1 cut(s)	328
PasI	CCCWGGG	1 cut(s)	1192
PceI	AGGCCT	1 cut(s)	1279
PdiI	GCCGGC	1 cut(s)	1530
PkrI	GCNGC	8 cut(s)	109, 139, 172, 361, 731, 1019, 1123, 1338
PleI	GAGTC	2 cut(s)	983, 1163
PpsI	GAGTC	2 cut(s)	983, 1163
PpuMI	RGGWCCY	1 cut(s)	83
Psp124BI	GAGCTC	1 cut(s)	69
Psp5II	RGGWCCY	1 cut(s)	83
Psp6I	CCWGG	2 cut(s)	1191, 1447
PspEI	GGTNACC	4 cut(s)	848, 1028, 1177, 1451
PspGI	CCWGG	2 cut(s)	1191, 1447
PspN4I	GGNNCC	3 cut(s)	84, 1093, 1120
PspPI	GGNCC	5 cut(s)	83, 953, 994, 1189, 1412
PspPPI	RGGWCCY	1 cut(s)	83
PspXI	VCTCGAGB	1 cut(s)	1152
PstI	CTGCAG	1 cut(s)	175
PstNI	CAGNNNCTG	2 cut(s)	704, 899
PsuI	RGATCY	2 cut(s)	337, 1204
RsaI	GTAC	4 cut(s)	686, 740, 1093, 1391
RsaNI	GTAC	4 cut(s)	685, 739, 1092, 1390
RseI	CAYNNNNRTG	3 cut(s)	53, 651, 782
SacI	GAGCTC	1 cut(s)	69
SacII	CCGCGG	1 cut(s)	1125
SaqAI	TTAA	5 cut(s)	156, 507, 615, 708, 1542
SatI	GCNGC	8 cut(s)	108, 138, 171, 360, 730, 1018, 1122, 1337
Sau3AI	GATC	9 cut(s)	88, 183, 337, 520, 568, 601, 631, 1204, 1230
Sau96I	GGNCC	5 cut(s)	83, 953, 994, 1189, 1412
SchI	GAGTC	2 cut(s)	983, 1164
ScrFI	CCNGG	4 cut(s)	952, 1193, 1416, 1449
SduI	GDGCHC	3 cut(s)	69, 1131, 1382
SfaNI	GCATC	4 cut(s)	643, 1219, 1430, 1459
SfcI	CTRYAG	1 cut(s)	171
Sfr274I	CTCGAG	1 cut(s)	1152
Sfr303I	CCGCGG	1 cut(s)	1125
SgrBI	CCGCGG	1 cut(s)	1125
SinI	GGWCC	2 cut(s)	83, 994
SlaI	CTCGAG	1 cut(s)	1152
SmiMI	CAYNNNNRTG	3 cut(s)	53, 651, 782
SmlI	CTYRAG	3 cut(s)	1152, 1321, 1352
SmoI	CTYRAG	3 cut(s)	1152, 1321, 1352
Sse9I	AATT	9 cut(s)	24, 303, 421, 488, 543, 574, 825, 1064, 1135
SseBI	AGGCCT	1 cut(s)	1279
SsiI	CCGC	5 cut(s)	678, 1122, 1124, 1160, 1208
SspMI	CTAG	4 cut(s)	1296, 1331, 1341, 1482
SstI	GAGCTC	1 cut(s)	69
StuI	AGGCCT	1 cut(s)	1279
StyD4I	CCNGG	4 cut(s)	950, 1191, 1414, 1447
StyI	CCWWGG	2 cut(s)	515, 1280
TaaI	ACNGT	8 cut(s)	599, 689, 838, 895, 1044, 1303, 1495, 1552
TaqI	TCGA	4 cut(s)	396, 417, 1153, 1229
TaqII	GACCGA	1 cut(s)	867
TasI	AATT	9 cut(s)	24, 303, 421, 488, 543, 574, 825, 1064, 1135
TauI	GCSGC	1 cut(s)	1124
Tru1I	TTAA	5 cut(s)	156, 507, 615, 708, 1542
Tru9I	TTAA	5 cut(s)	156, 507, 615, 708, 1542
TscAI	CASTG	2 cut(s)	1047, 1225
TseFI	GTSAC	7 cut(s)	314, 448, 593, 848, 1028, 1079, 1177
TseI	GCWGC	7 cut(s)	107, 137, 170, 359, 729, 1017, 1336
Tsp45I	GTSAC	7 cut(s)	314, 448, 593, 848, 1028, 1079, 1177
TspRI	CASTG	2 cut(s)	1047, 1225
VpaK11BI	GGWCC	2 cut(s)	83, 994
XapI	RAATTY	2 cut(s)	303, 421
XceI	RCATGY	3 cut(s)	384, 497, 650
XhoI	CTCGAG	1 cut(s)	1152
XmiI	GTMKAC	1 cut(s)	482
XspI	CTAG	4 cut(s)	1296, 1331, 1341, 1482
Zsp2I	ATGCAT	1 cut(s)	648

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.