FvH4_6g07320

Cyclic phosphodiesterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009695
Physical Location & Seq
Reverse (-)
4376876 .. 4380871
3996 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g07320.t1

Sequence Viewer

Length: 1524 bp
ATGGAGATTTTGCGACCCAAACGTGCGAGACTCAAGAAGAAGAATGTATTTGTAGACCGTTGGGATGGGAGGGATTGGAAAGATGAAATAATTTGGGAGAAAGAGGTGGTTGATTGGTGCCAACTTGTCATCCATGACGATGCCAAACCTGTGACAAACCTGTTGGTTTGCTCAATGTGGATGGGTACTACAACTTACTCTTATCTTTTATTAACACTGCCGTGGATGAAGGCGTGTTATTCAGCCTTTCAAGCACGCCACATCTTAGTAGGCACAGCTGCCATTGACTTAGGCAAAGAGCTGGTGTCAAGGAAGGTGGACCTTGTCTATGGAGCTGGAAGAATTGTACTGATGGGTTGTGTTTGTGCAAGATGGTGGAGGATCATCCCCAGGCCTCTAATAGGTCCACAGATAACGGGTGAAACAGTTTGGAAGGAAAGGTCTGTAACCAATATGCATCAAAGGAAGGCTGAAATGGCCAGCAATTCTGATTGTTTTAAGGCATTACCAGGAGGGTATGGAACTCTTGAAGAGTTGTTCCATGACAAACCTGTGAGAGAGCATATTATGATACTGATAAACCCACCAATGCGAACTGGAACTTTCGCGCGCCATTTCAGTCACATTGGATTGAGGCGAAGTTTTCGGTGGTATTCCACATCTGAGAAAAGAGCTCAAGAAAAAGTTAGTGTTCTGGACGAGACCATTAGCCGCCTCAATTTCACCATAGGTCGCCTTGCACTATACAAATGTAACAATGAATACAGAACTCAACACTCATGGGAGAAGATTCGTGATCTAACTTCCCCTTCATTAGGTTGTATTTCAATATTTGAGCCTGGCAACCATGATCATCCATTTTGTGTGCGAATGAGAAATAATTTCCTTCCTGAGAAATTTGATCCTGTCATTTTCTCTGTTCTATCACTACTCTTCTCCATTGTGATGCCATCCCAAGAACAAGAAGCATTAGAAGCATTCAAGGATGCGCAGGACATGCACATATATGCTGTGTGGGCTCTTCCGCCCACACATGTGCTCCCGCGAATCAAAAATGTGATAGAGGGCTTACGGGACGAATTTGGTGGGCCGGAAATTGAACCCCATATCACCGTCGTGGGATCTATGCTGAAGACAGAAGAAGATGCGACCCGGCAATTCAAAGATGCTTGTGACATTATCACCCGTTTTGAATGTACCGTCGATCACATTGAAACTAGTCGGTTTTACTACCAGTGTGTCTCCCTCATTATTGATCCAATTGAAGACTTATGGGGTGAAATTGGACGCTTTGTTAGGATCTTCGAACACACTTTCCCTTGTTGTCCACATTTGGACATCCTCTATGGGTATCTAACAGATGAAGAGAAAGAACGAGCTAAAGAGAAAGTCACTACTATCGATGAGGGCATTGCTACCCTTAGCTTCTCGGTTACTCGCTTTGTATTATGCAAGATGATCAACGATGATAAATCTCAAAACTCTTGGGAGAAGATTATGGCAATTGACATCCAACCAGAATAA

Protein Analysis

508

Amino Acids

58.8

Weight (kDa)

6.37

Isoelectric Point (pI)

47.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lysine_decarbox PF03641 128 - 180 2.6e-11 Possible lysine decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 990
AccB1I GGYRCC 1 cut(s) 117
AccI GTMKAC 1 cut(s) 54
AccII CGCG 3 cut(s) 608, 610, 1045
AciI CCGC 3 cut(s) 712, 1025, 1043
AclWI GGATC 5 cut(s) 389, 896, 1129, 1250, 1307
AcoI YGGCCR 1 cut(s) 477
AcsI RAATTY 2 cut(s) 896, 1079
AcuI CTGAAG 1 cut(s) 1151
AfaI GTAC 3 cut(s) 187, 348, 1198
AfiI CCNNNNNNNGG 2 cut(s) 401, 815
AflIII ACRYGT 1 cut(s) 1033
AgsI TTSAA 9 cut(s) 251, 530, 828, 982, 1100, 1162, 1193, 1214, 1265
AhlI ACTAGT 1 cut(s) 1217
AjnI CCWGG 3 cut(s) 389, 508, 838
AleI CACNNNNGTG 3 cut(s) 220, 1034, 1115
AloI GAACNNNNNNTCC 2 cut(s) 1299, 1331
AluBI AGCT 6 cut(s) 278, 301, 335, 674, 1379, 1425
AluI AGCT 6 cut(s) 278, 301, 335, 674, 1379, 1425
Alw21I GWGCWC 2 cut(s) 676, 1041
Alw26I GTCTC 3 cut(s) 22, 695, 1246
AlwI GGATC 5 cut(s) 389, 896, 1129, 1250, 1307
AoxI GGCC 3 cut(s) 392, 477, 1088
ApeKI GCWGC 1 cut(s) 278
ApoI RAATTY 2 cut(s) 896, 1079
AspLEI GCGC 3 cut(s) 610, 612, 991
AspS9I GGNCC 3 cut(s) 319, 404, 1088
AsuC2I CCSGG 1 cut(s) 1153
AsuHPI GGTGA 5 cut(s) 431, 715, 1102, 1174, 1289
AsuII TTCGAA 1 cut(s) 1305
AvaII GGWCC 2 cut(s) 319, 404
BalI TGGCCA 1 cut(s) 479
BanI GGYRCC 1 cut(s) 117
BanII GRGCYC 2 cut(s) 676, 1021
BbsI GAAGAC 2 cut(s) 1139, 1272
Bbv12I GWGCWC 2 cut(s) 676, 1041
BbvI GCAGC 1 cut(s) 265
BccI CCATC 5 cut(s) 59, 175, 346, 366, 958
BceAI ACGGC 1 cut(s) 205
BciT130I CCWGG 3 cut(s) 391, 510, 840
BclI TGATCA 2 cut(s) 850, 1458
BcnI CCSGG 1 cut(s) 1153
BcoDI GTCTC 3 cut(s) 22, 695, 1246
BcuI ACTAGT 1 cut(s) 1217
BfaI CTAG 1 cut(s) 1218
BisI GCNGC 2 cut(s) 279, 712
BlsI GCNGC 2 cut(s) 280, 713
Bme1390I CCNGG 4 cut(s) 391, 510, 840, 1153
Bme18I GGWCC 2 cut(s) 319, 404
BmgT120I GGNCC 3 cut(s) 319, 404, 1088
BmiI GGNNCC 1 cut(s) 119
BmrFI CCNGG 4 cut(s) 391, 510, 840, 1153
BmsI GCATC 6 cut(s) 130, 466, 936, 976, 1135, 1156
BpiI GAAGAC 2 cut(s) 1139, 1272
Bpu10I CCTNAGC 1 cut(s) 1421
Bpu14I TTCGAA 1 cut(s) 1305
BpuEI CTTGAG 2 cut(s) 17, 660
BpuMI CCSGG 1 cut(s) 1153
Bsa29I ATCGAT 1 cut(s) 1401
BsaI GGTCTC 1 cut(s) 695
BsaJI CCNNGG 2 cut(s) 221, 389
BsaXI ACNNNNNCTCC 2 cut(s) 1023, 1053
Bsc4I CCNNNNNNNGG 2 cut(s) 401, 815
Bse1I ACTGG 2 cut(s) 601, 1234
Bse3DI GCAATG 1 cut(s) 1410
BseBI CCWGG 3 cut(s) 391, 510, 840
BseCI ATCGAT 1 cut(s) 1401
BseDI CCNNGG 2 cut(s) 221, 389
BseLI CCNNNNNNNGG 2 cut(s) 401, 815
BseMI GCAATG 1 cut(s) 1410
BseMII CTCAG 2 cut(s) 654, 882
BseNI ACTGG 2 cut(s) 601, 1234
BsePI GCGCGC 1 cut(s) 608
BseXI GCAGC 1 cut(s) 265
Bsh1236I CGCG 3 cut(s) 608, 610, 1045
BshFI GGCC 3 cut(s) 394, 479, 1090
BshNI GGYRCC 1 cut(s) 117
BshVI ATCGAT 1 cut(s) 1401
BsiHKAI GWGCWC 2 cut(s) 676, 1041
BsiSI CCGG 2 cut(s) 1091, 1153
BslFI GGGAC 1 cut(s) 1088
BslI CCNNNNNNNGG 2 cut(s) 401, 815
BsmAI GTCTC 3 cut(s) 22, 695, 1246
BsmFI GGGAC 1 cut(s) 1088
BsmI GAATGC 1 cut(s) 977
BsnI GGCC 3 cut(s) 394, 479, 1090
Bso31I GGTCTC 1 cut(s) 695
Bsp119I TTCGAA 1 cut(s) 1305
Bsp1286I GDGCHC 3 cut(s) 676, 1021, 1041
Bsp143I GATC 9 cut(s) 381, 796, 850, 901, 1121, 1204, 1255, 1299, 1458
BspACI CCGC 3 cut(s) 712, 1025, 1043
BspANI GGCC 3 cut(s) 394, 479, 1090
BspCNI CTCAG 2 cut(s) 655, 883
BspDI ATCGAT 1 cut(s) 1401
BspFNI CGCG 3 cut(s) 608, 610, 1045
BspLI GGNNCC 1 cut(s) 119
BspPI GGATC 5 cut(s) 389, 896, 1129, 1250, 1307
BspQI GCTCTTC 1 cut(s) 1026
BspT104I TTCGAA 1 cut(s) 1305
BspT107I GGYRCC 1 cut(s) 117
BspTNI GGTCTC 1 cut(s) 695
BsrDI GCAATG 1 cut(s) 1410
BsrI ACTGG 2 cut(s) 601, 1234
BssECI CCNNGG 2 cut(s) 221, 389
BssHII GCGCGC 1 cut(s) 608
BssMI GATC 9 cut(s) 381, 796, 850, 901, 1121, 1204, 1255, 1299, 1458
Bst2UI CCWGG 3 cut(s) 391, 510, 840
Bst4CI ACNGT 4 cut(s) 59, 427, 1114, 1201
Bst6I CTCTTC 4 cut(s) 525, 938, 1026, 1359
BstAPI GCANNNNNTGC 1 cut(s) 997
BstBI TTCGAA 1 cut(s) 1305
BstC8I GCNNGC 3 cut(s) 256, 481, 610
BstDEI CTNAG 5 cut(s) 265, 289, 663, 891, 1421
BstDSI CCRYGG 1 cut(s) 221
BstENI CCTNNNNNAGG 2 cut(s) 399, 813
BstFNI CGCG 3 cut(s) 608, 610, 1045
BstHHI GCGC 3 cut(s) 610, 612, 991
BstKTI GATC 9 cut(s) 384, 799, 853, 904, 1124, 1207, 1258, 1302, 1461
BstMAI GTCTC 3 cut(s) 22, 695, 1246
BstMBI GATC 9 cut(s) 381, 796, 850, 901, 1121, 1204, 1255, 1299, 1458
BstMWI GCNNNNNNNGC 5 cut(s) 251, 476, 974, 997, 1016
BstNI CCWGG 3 cut(s) 391, 510, 840
BstNSI RCATGY 2 cut(s) 1000, 1037
BstSCI CCNGG 4 cut(s) 389, 508, 838, 1151
BstUI CGCG 3 cut(s) 608, 610, 1045
BstV1I GCAGC 1 cut(s) 265
BstV2I GAAGAC 2 cut(s) 1139, 1272
BstX2I RGATCY 2 cut(s) 1121, 1299
BstYI RGATCY 2 cut(s) 1121, 1299
Bsu15I ATCGAT 1 cut(s) 1401
BsuRI GGCC 3 cut(s) 394, 479, 1090
BsuTUI ATCGAT 1 cut(s) 1401
BtgI CCRYGG 1 cut(s) 221
BtsI GCAGTG 1 cut(s) 215
BtsIMutI CAGTG 2 cut(s) 215, 1241
Cac8I GCNNGC 3 cut(s) 256, 481, 610
CfoI GCGC 3 cut(s) 610, 612, 991
Cfr13I GGNCC 3 cut(s) 319, 404, 1088
ClaI ATCGAT 1 cut(s) 1401
CseI GACGC 1 cut(s) 1296
Csp6I GTAC 3 cut(s) 186, 347, 1197
CspCI CAANNNNNGTGG 2 cut(s) 297, 332
CviAII CATG 6 cut(s) 134, 542, 780, 848, 997, 1034
CviQI GTAC 3 cut(s) 186, 347, 1197
DdeI CTNAG 5 cut(s) 265, 289, 663, 891, 1421
DpnI GATC 9 cut(s) 383, 798, 852, 903, 1123, 1206, 1257, 1301, 1460
DpnII GATC 9 cut(s) 381, 796, 850, 901, 1121, 1204, 1255, 1299, 1458
EaeI YGGCCR 1 cut(s) 477
Eam1104I CTCTTC 4 cut(s) 525, 938, 1026, 1359
EarI CTCTTC 4 cut(s) 525, 938, 1026, 1359
EciI GGCGGA 1 cut(s) 1014
Ecl136II GAGCTC 1 cut(s) 674
Eco147I AGGCCT 1 cut(s) 394
Eco24I GRGCYC 2 cut(s) 676, 1021
Eco31I GGTCTC 1 cut(s) 695
Eco47I GGWCC 2 cut(s) 319, 404
Eco53kI GAGCTC 1 cut(s) 674
Eco57I CTGAAG 1 cut(s) 1151
EcoICRI GAGCTC 1 cut(s) 674
EcoNI CCTNNNNNAGG 2 cut(s) 399, 813
EcoRII CCWGG 3 cut(s) 389, 508, 838
EcoT22I ATGCAT 1 cut(s) 459
EcoT38I GRGCYC 2 cut(s) 676, 1021
FaeI CATG 6 cut(s) 137, 545, 783, 851, 1000, 1037
FaqI GGGAC 1 cut(s) 1088
FatI CATG 6 cut(s) 133, 541, 779, 847, 996, 1033
FauI CCCGC 1 cut(s) 1050
FbaI TGATCA 2 cut(s) 850, 1458
FblI GTMKAC 1 cut(s) 54
Fnu4HI GCNGC 2 cut(s) 279, 712
FriOI GRGCYC 2 cut(s) 676, 1021
Fsp4HI GCNGC 2 cut(s) 279, 712
FspBI CTAG 1 cut(s) 1218
FspI TGCGCA 1 cut(s) 990
GlaI GCGC 3 cut(s) 609, 611, 990
GluI GCNGC 2 cut(s) 279, 712
HaeIII GGCC 3 cut(s) 394, 479, 1090
HapII CCGG 2 cut(s) 1091, 1153
HgaI GACGC 1 cut(s) 1296
HhaI GCGC 3 cut(s) 610, 612, 991
Hin1II CATG 6 cut(s) 137, 545, 783, 851, 1000, 1037
Hin6I GCGC 3 cut(s) 608, 610, 989
HinP1I GCGC 3 cut(s) 608, 610, 989
HinfI GANTC 3 cut(s) 30, 790, 1047
HpaII CCGG 2 cut(s) 1091, 1153
HphI GGTGA 5 cut(s) 431, 715, 1102, 1174, 1289
Hpy166II GTNNAC 4 cut(s) 55, 319, 407, 1328
Hpy188I TCNGA 2 cut(s) 490, 664
Hpy188III TCNNGA 6 cut(s) 34, 527, 677, 695, 794, 890
Hpy8I GTNNAC 4 cut(s) 55, 319, 407, 1328
Hpy99I CGWCG 2 cut(s) 1118, 1205
HpyAV CCTTC 6 cut(s) 223, 307, 427, 460, 819, 896
HpyCH4III ACNGT 4 cut(s) 59, 427, 1114, 1201
HpyCH4IV ACGT 1 cut(s) 22
HpyCH4V TGCA 5 cut(s) 368, 457, 740, 1000, 1452
HpyF10VI GCNNNNNNNGC 5 cut(s) 251, 476, 974, 997, 1016
HpyF3I CTNAG 5 cut(s) 265, 289, 663, 891, 1421
HpySE526I ACGT 1 cut(s) 22
Hsp92II CATG 6 cut(s) 137, 545, 783, 851, 1000, 1037
HspAI GCGC 3 cut(s) 608, 610, 989
Ksp22I TGATCA 2 cut(s) 850, 1458
Kzo9I GATC 9 cut(s) 381, 796, 850, 901, 1121, 1204, 1255, 1299, 1458
LguI GCTCTTC 1 cut(s) 1026
LmnI GCTCC 2 cut(s) 332, 1044
Lsp1109I GCAGC 1 cut(s) 265
LweI GCATC 6 cut(s) 130, 466, 936, 976, 1135, 1156
MaeI CTAG 1 cut(s) 1218
MaeII ACGT 1 cut(s) 22
MaeIII GTNAC 7 cut(s) 151, 445, 620, 752, 1172, 1390, 1432
MalI GATC 9 cut(s) 383, 798, 852, 903, 1123, 1206, 1257, 1301, 1460
MboI GATC 9 cut(s) 381, 796, 850, 901, 1121, 1204, 1255, 1299, 1458
MfeI CAATTG 2 cut(s) 1260, 1503
MflI RGATCY 2 cut(s) 1121, 1299
MhlI GDGCHC 3 cut(s) 676, 1021, 1041
MlsI TGGCCA 1 cut(s) 479
MluNI TGGCCA 1 cut(s) 479
MlyI GAGTC 1 cut(s) 24
Mox20I TGGCCA 1 cut(s) 479
Mph1103I ATGCAT 1 cut(s) 459
MscI TGGCCA 1 cut(s) 479
MseI TTAA 2 cut(s) 212, 498
MslI CAYNNNNRTG 6 cut(s) 138, 220, 944, 1005, 1034, 1115
Msp20I TGGCCA 1 cut(s) 479
MspA1I CMGCKG 1 cut(s) 278
MspI CCGG 2 cut(s) 1091, 1153
MspR9I CCNGG 4 cut(s) 391, 510, 840, 1153
MunI CAATTG 2 cut(s) 1260, 1503
Mva1269I GAATGC 1 cut(s) 977
MvaI CCWGG 3 cut(s) 391, 510, 840
MvnI CGCG 3 cut(s) 608, 610, 1045
MwoI GCNNNNNNNGC 5 cut(s) 251, 476, 974, 997, 1016
NciI CCSGG 1 cut(s) 1153
NdeII GATC 9 cut(s) 381, 796, 850, 901, 1121, 1204, 1255, 1299, 1458
NlaIII CATG 6 cut(s) 137, 545, 783, 851, 1000, 1037
NlaIV GGNNCC 1 cut(s) 119
NmuCI GTSAC 4 cut(s) 151, 620, 1172, 1390
NsbI TGCGCA 1 cut(s) 990
NsiI ATGCAT 1 cut(s) 459
NspI RCATGY 2 cut(s) 1000, 1037
NspV TTCGAA 1 cut(s) 1305
OliI CACNNNNGTG 3 cut(s) 220, 1034, 1115
PauI GCGCGC 1 cut(s) 608
PceI AGGCCT 1 cut(s) 394
PciI ACATGT 1 cut(s) 1033
PciSI GCTCTTC 1 cut(s) 1026
PctI GAATGC 1 cut(s) 977
PfeI GAWTC 2 cut(s) 790, 1047
PflFI GACNNNGTC 1 cut(s) 323
PkrI GCNGC 2 cut(s) 280, 713
PleI GAGTC 1 cut(s) 24
PpsI GAGTC 1 cut(s) 24
PscI ACATGT 1 cut(s) 1033
Psp124BI GAGCTC 1 cut(s) 676
Psp6I CCWGG 3 cut(s) 389, 508, 838
PspGI CCWGG 3 cut(s) 389, 508, 838
PspN4I GGNNCC 1 cut(s) 119
PspPI GGNCC 3 cut(s) 319, 404, 1088
PsuI RGATCY 2 cut(s) 1121, 1299
PsyI GACNNNGTC 1 cut(s) 323
PteI GCGCGC 1 cut(s) 608
PvuII CAGCTG 1 cut(s) 278
RsaI GTAC 3 cut(s) 187, 348, 1198
RsaNI GTAC 3 cut(s) 186, 347, 1197
RseI CAYNNNNRTG 6 cut(s) 138, 220, 944, 1005, 1034, 1115
SacI GAGCTC 1 cut(s) 676
SapI GCTCTTC 1 cut(s) 1026
SaqAI TTAA 2 cut(s) 212, 498
SatI GCNGC 2 cut(s) 279, 712
Sau3AI GATC 9 cut(s) 381, 796, 850, 901, 1121, 1204, 1255, 1299, 1458
Sau96I GGNCC 3 cut(s) 319, 404, 1088
SchI GAGTC 1 cut(s) 24
ScrFI CCNGG 4 cut(s) 391, 510, 840, 1153
SduI GDGCHC 3 cut(s) 676, 1021, 1041
SfaNI GCATC 6 cut(s) 130, 466, 936, 976, 1135, 1156
SfuI TTCGAA 1 cut(s) 1305
SinI GGWCC 2 cut(s) 319, 404
SmiMI CAYNNNNRTG 6 cut(s) 138, 220, 944, 1005, 1034, 1115
SmlI CTYRAG 2 cut(s) 32, 675
SmoI CTYRAG 2 cut(s) 32, 675
SpeI ACTAGT 1 cut(s) 1217
SseBI AGGCCT 1 cut(s) 394
SsiI CCGC 3 cut(s) 712, 1025, 1043
SspI AATATT 1 cut(s) 831
SspMI CTAG 1 cut(s) 1218
SstI GAGCTC 1 cut(s) 676
StuI AGGCCT 1 cut(s) 394
StyD4I CCNGG 4 cut(s) 389, 508, 838, 1151
TaaI ACNGT 4 cut(s) 59, 427, 1114, 1201
TaiI ACGT 1 cut(s) 25
TaqI TCGA 3 cut(s) 1203, 1305, 1401
TatI WGTACW 1 cut(s) 346
TauI GCSGC 1 cut(s) 714
TfiI GAWTC 2 cut(s) 790, 1047
Tru1I TTAA 2 cut(s) 212, 498
Tru9I TTAA 2 cut(s) 212, 498
TscAI CASTG 2 cut(s) 222, 1241
TseFI GTSAC 4 cut(s) 151, 620, 1172, 1390
TseI GCWGC 1 cut(s) 278
Tsp45I GTSAC 4 cut(s) 151, 620, 1172, 1390
TspDTI ATGAA 5 cut(s) 99, 242, 774, 801, 1377
TspRI CASTG 2 cut(s) 222, 1241
Tth111I GACNNNGTC 1 cut(s) 323
VpaK11BI GGWCC 2 cut(s) 319, 404
XagI CCTNNNNNAGG 2 cut(s) 399, 813
XapI RAATTY 2 cut(s) 896, 1079
XceI RCATGY 2 cut(s) 1000, 1037
XmiI GTMKAC 1 cut(s) 54
XspI CTAG 1 cut(s) 1218
Zsp2I ATGCAT 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.