FvH4_6g10440

Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009236
Physical Location & Seq
Reverse (-)
6257956 .. 6262255
4300 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g10440.t1

Sequence Viewer

Length: 1446 bp
ATGCAGGGATTTATAGGGAGAAGGTTAATATCGGGAACGAGACAATTATCTAATCTCCGACCATCCTCGCAACGCTGTTGTTTCTCTCAGGTGGCTCAGAGAGAGGAGGCCAACAATGGCGTCCATATTCCCAAACTGCCCCCTTTCGACTACTCTCCTCCGCCGTACACCGGTCCCTCCACAGAGGAGATCTTGGCTCAGAGGAAAGAGTACCTCAGCCCCTCCATGTTCTACTTCTACAACAAACCCCTGAATATTGTCGACGGGAAGATGCAGTATCTGTTCGATGAGAATGGTCGGAGGTATTTGGACGGTTTCGGAGGGATAGCTACGGTGAGTTGCGGCCACTGCCACCCTGATGTGGTGGAGGCCATAGTCAACCAAACCAAGAAAATCCAACACTCGACGATTCTTTACCTGAATCATGCAATCGGCGATTTCGCTCAGGCACTGGCTTCCAAGTTGCCCGCCAATCTCAAGGTAGTGTTCTTCACTAATTCAGGGACGGAAGCAAACGAGTTGGCGATGTTGATAGCGAGGTTGTACACAGGCTGCCATGACATAATCTCTTTGAGAAACGCATATCATGGCAACGCTTCTGGCACTATGGGCGCCACTGCCCAAGCCAGTTGGAAATTTAACGTCATTCAGAGTGGAGTTCATCATGCTATGAATCCGGACCCATACAGAGGTGTGTTTGGTTCAGATGGTGAGAAGTATGCCAAAGATGTCCAAGATCTCATCGACTTTGGAACCTCTGGCCATGTTGCTGGCTTTATATCTGAAGCCATACAGGGAGTGGGAGGAATTGTAGAATTGGCCCCGGGTTTCTTGCCAGCAGTTTATAACAGTGTAAAGAAAGCAGGAGGCCTATTCATCGCCGATGAGGTCCAGTCTGGTTTTGCTCGCACAGGAAGCAACTTCTGGGGGTTTGAGGGTCATGGTGTTGTACCTGACATTGTGACAATGGCCAAGGGCATTGGAAATGGCACTCCCCTTGGTGCAGTAGTAACCACTCCTGAGGTTGCAGAGGTATTGACTCGCCGCAATTACTTTAACACCTTCGGTGGCAATCCTGTATGCACGGCTGCAGGACTGGCTGTTCTGAAAGTGATTGAGAAAGAAGGACTTCAAGAAAATGCATTTGTTGTGGGCTCATATCTGAAAGAAAGACTCAATGCCTTGAAAGATAAATATGAACTTATCGGAGATGTGAGGGGAAAAGGATTAATGCTAGGAGTTGAGCTTGTGACTGATCGTCAACTGAAGACACCAGCCAAGGCTGAAACTATACATGTGATGGACAAAATGAAAGATTTGGGAGTCTTGATCGGCAAAGGGGGATCCTATGGAAATGTTTTCAGAATCACACCTCCCTTGTGCTTCACTAAGGAAGATGCAGATTTTCTTGTGGATGCAATGGACTACACAATGTCTAAAGTTTGA

Protein Analysis

482

Amino Acids

52.18

Weight (kDa)

6.87

Isoelectric Point (pI)

30.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_3 PF00202 84 - 475 1e-95 Aminotransferase class-III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 846
AccB1I GGYRCC 1 cut(s) 611
AccI GTMKAC 1 cut(s) 261
AccIII TCCGGA 1 cut(s) 676
AciI CCGC 4 cut(s) 161, 342, 468, 1045
AclWI GGATC 2 cut(s) 1338, 1351
AcoI YGGCCR 3 cut(s) 343, 760, 969
AcsI RAATTY 1 cut(s) 635
AcuI CTGAAG 2 cut(s) 804, 1286
AcyI GRCGYC 2 cut(s) 120, 612
AfaI GTAC 4 cut(s) 167, 212, 545, 951
AfiI CCNNNNNNNGG 3 cut(s) 170, 361, 689
AflIII ACRYGT 1 cut(s) 1294
AgeI ACCGGT 1 cut(s) 170
AgsI TTSAA 2 cut(s) 1133, 1186
AhdI GACNNNNNGTC 1 cut(s) 1257
AloI GAACNNNNNNTCC 2 cut(s) 1086, 1118
AluBI AGCT 2 cut(s) 329, 1246
AluI AGCT 2 cut(s) 329, 1246
Alw26I GTCTC 1 cut(s) 34
AlwI GGATC 2 cut(s) 1338, 1351
AlwNI CAGNNNCTG 2 cut(s) 280, 451
Ama87I CYCGRG 1 cut(s) 823
Aor13HI TCCGGA 1 cut(s) 676
AoxI GGCC 7 cut(s) 108, 343, 369, 760, 819, 868, 969
ApeKI GCWGC 2 cut(s) 552, 1088
ApoI RAATTY 1 cut(s) 635
AseI ATTAAT 1 cut(s) 1229
AsiGI ACCGGT 1 cut(s) 170
Asp700I GAANNNNTTC 1 cut(s) 1128
AspLEI GCGC 1 cut(s) 614
AspS9I GGNCC 4 cut(s) 173, 679, 820, 889
AsuC2I CCSGG 2 cut(s) 824, 825
AsuHPI GGTGA 2 cut(s) 346, 722
AvaI CYCGRG 1 cut(s) 823
AvaII GGWCC 3 cut(s) 173, 679, 889
AxyI CCTNAGG 1 cut(s) 1020
BalI TGGCCA 2 cut(s) 762, 971
BamHI GGATCC 1 cut(s) 1343
BanI GGYRCC 1 cut(s) 611
BanII GRGCYC 1 cut(s) 1157
BbsI GAAGAC 1 cut(s) 1274
BbvCI CCTCAGC 1 cut(s) 215
BbvI GCAGC 2 cut(s) 539, 1075
BccI CCATC 3 cut(s) 70, 701, 1294
BceAI ACGGC 2 cut(s) 148, 1101
BcnI CCSGG 2 cut(s) 824, 825
BcoDI GTCTC 1 cut(s) 34
BfaI CTAG 1 cut(s) 1235
BfmI CTRYAG 1 cut(s) 1089
BfoI RGCGCY 1 cut(s) 615
BglII AGATCT 2 cut(s) 189, 736
BisI GCNGC 4 cut(s) 343, 553, 1045, 1089
BlsI GCNGC 4 cut(s) 344, 554, 1046, 1090
Bme1390I CCNGG 2 cut(s) 824, 825
Bme18I GGWCC 3 cut(s) 173, 679, 889
BmeRI GACNNNNNGTC 1 cut(s) 1257
BmeT110I CYCGRG 1 cut(s) 823
BmgT120I GGNCC 4 cut(s) 173, 679, 820, 889
BmiI GGNNCC 6 cut(s) 175, 613, 681, 754, 822, 1345
BmrFI CCNGG 2 cut(s) 824, 825
BmsI GCATC 3 cut(s) 261, 1387, 1405
BpiI GAAGAC 1 cut(s) 1274
Bpu10I CCTNAGC 2 cut(s) 215, 444
BpuEI CTTGAG 1 cut(s) 461
BpuMI CCSGG 2 cut(s) 824, 825
BsaHI GRCGYC 2 cut(s) 120, 612
BsaJI CCNNGG 5 cut(s) 822, 823, 972, 997, 1278
BsaWI WCCGGW 2 cut(s) 170, 676
BsaXI ACNNNNNCTCC 2 cut(s) 795, 825
Bsc4I CCNNNNNNNGG 3 cut(s) 170, 361, 689
Bse118I RCCGGY 1 cut(s) 170
Bse1I ACTGG 4 cut(s) 456, 627, 892, 1101
Bse21I CCTNAGG 1 cut(s) 1020
Bse3DI GCAATG 1 cut(s) 1425
BseAI TCCGGA 1 cut(s) 676
BseDI CCNNGG 5 cut(s) 822, 823, 972, 997, 1278
BseGI GGATG 2 cut(s) 62, 1420
BseLI CCNNNNNNNGG 3 cut(s) 170, 361, 689
BseMI GCAATG 1 cut(s) 1425
BseMII CTCAG 6 cut(s) 101, 110, 212, 229, 458, 1011
BseNI ACTGG 4 cut(s) 456, 627, 892, 1101
BseRI GAGGAG 3 cut(s) 119, 147, 200
BseXI GCAGC 2 cut(s) 539, 1075
BsgI GTGCAG 1 cut(s) 1023
BshFI GGCC 7 cut(s) 110, 345, 371, 762, 821, 870, 971
BshNI GGYRCC 1 cut(s) 611
BshTI ACCGGT 1 cut(s) 170
BsiHKCI CYCGRG 1 cut(s) 823
BsiSI CCGG 3 cut(s) 171, 677, 824
BslFI GGGAC 2 cut(s) 159, 517
BslI CCNNNNNNNGG 3 cut(s) 170, 361, 689
BsmAI GTCTC 1 cut(s) 34
BsmFI GGGAC 2 cut(s) 159, 517
BsnI GGCC 7 cut(s) 110, 345, 371, 762, 821, 870, 971
BsoBI CYCGRG 1 cut(s) 823
Bsp1286I GDGCHC 1 cut(s) 1157
Bsp13I TCCGGA 1 cut(s) 676
Bsp1407I TGTACA 1 cut(s) 543
Bsp143I GATC 5 cut(s) 189, 736, 1255, 1329, 1343
BspACI CCGC 4 cut(s) 161, 342, 468, 1045
BspANI GGCC 7 cut(s) 110, 345, 371, 762, 821, 870, 971
BspCNI CTCAG 6 cut(s) 100, 109, 211, 228, 457, 1012
BspEI TCCGGA 1 cut(s) 676
BspLI GGNNCC 6 cut(s) 175, 613, 681, 754, 822, 1345
BspMAI CTGCAG 1 cut(s) 1093
BspPI GGATC 2 cut(s) 1338, 1351
BspT107I GGYRCC 1 cut(s) 611
BsrDI GCAATG 1 cut(s) 1425
BsrFI RCCGGY 1 cut(s) 170
BsrGI TGTACA 1 cut(s) 543
BsrI ACTGG 4 cut(s) 456, 627, 892, 1101
BssAI RCCGGY 1 cut(s) 170
BssECI CCNNGG 5 cut(s) 822, 823, 972, 997, 1278
BssMI GATC 5 cut(s) 189, 736, 1255, 1329, 1343
BssNI GRCGYC 2 cut(s) 120, 612
BssT1I CCWWGG 3 cut(s) 972, 997, 1278
Bst4CI ACNGT 3 cut(s) 314, 334, 851
BstACI GRCGYC 2 cut(s) 120, 612
BstAUI TGTACA 1 cut(s) 543
BstC8I GCNNGC 4 cut(s) 468, 772, 837, 907
BstDEI CTNAG 7 cut(s) 87, 96, 198, 215, 444, 1020, 1389
BstF5I GGATG 2 cut(s) 62, 1420
BstH2I RGCGCY 1 cut(s) 615
BstHHI GCGC 1 cut(s) 614
BstKTI GATC 5 cut(s) 192, 739, 1258, 1332, 1346
BstMAI GTCTC 1 cut(s) 34
BstMBI GATC 5 cut(s) 189, 736, 1255, 1329, 1343
BstMWI GCNNNNNNNGC 4 cut(s) 348, 609, 915, 1097
BstNSI RCATGY 1 cut(s) 1298
BstSCI CCNGG 2 cut(s) 822, 823
BstSFI CTRYAG 1 cut(s) 1089
BstV1I GCAGC 2 cut(s) 539, 1075
BstV2I GAAGAC 1 cut(s) 1274
BstX2I RGATCY 3 cut(s) 189, 736, 1343
BstXI CCANNNNNNTGG 1 cut(s) 770
BstYI RGATCY 3 cut(s) 189, 736, 1343
Bsu36I CCTNAGG 1 cut(s) 1020
BsuRI GGCC 7 cut(s) 110, 345, 371, 762, 821, 870, 971
BtgZI GCGATG 2 cut(s) 539, 862
BtsCI GGATG 2 cut(s) 62, 1420
BtsI GCAGTG 2 cut(s) 346, 615
BtsIMutI CAGTG 4 cut(s) 346, 449, 615, 856
Cac8I GCNNGC 4 cut(s) 468, 772, 837, 907
CaiI CAGNNNCTG 2 cut(s) 280, 451
CfoI GCGC 1 cut(s) 614
Cfr10I RCCGGY 1 cut(s) 170
Cfr13I GGNCC 4 cut(s) 173, 679, 820, 889
Cfr9I CCCGGG 1 cut(s) 823
CseI GACGC 1 cut(s) 109
Csp6I GTAC 4 cut(s) 166, 211, 544, 950
CspAI ACCGGT 1 cut(s) 170
CviAII CATG 8 cut(s) 226, 425, 557, 587, 665, 764, 941, 1295
CviQI GTAC 4 cut(s) 166, 211, 544, 950
DdeI CTNAG 7 cut(s) 87, 96, 198, 215, 444, 1020, 1389
DinI GGCGCC 1 cut(s) 613
DpnI GATC 5 cut(s) 191, 738, 1257, 1331, 1345
DpnII GATC 5 cut(s) 189, 736, 1255, 1329, 1343
DriI GACNNNNNGTC 1 cut(s) 1257
EaeI YGGCCR 3 cut(s) 343, 760, 969
Eam1105I GACNNNNNGTC 1 cut(s) 1257
EciI GGCGGA 1 cut(s) 150
Eco130I CCWWGG 3 cut(s) 972, 997, 1278
Eco147I AGGCCT 1 cut(s) 870
Eco24I GRGCYC 1 cut(s) 1157
Eco47I GGWCC 3 cut(s) 173, 679, 889
Eco57I CTGAAG 2 cut(s) 804, 1286
Eco81I CCTNAGG 1 cut(s) 1020
Eco88I CYCGRG 1 cut(s) 823
EcoT14I CCWWGG 3 cut(s) 972, 997, 1278
EcoT22I ATGCAT 1 cut(s) 1144
EcoT38I GRGCYC 1 cut(s) 1157
EgeI GGCGCC 1 cut(s) 613
EheI GGCGCC 1 cut(s) 613
ErhI CCWWGG 3 cut(s) 972, 997, 1278
FaeI CATG 8 cut(s) 229, 428, 560, 590, 668, 767, 944, 1298
FalI AAGNNNNNCTT 2 cut(s) 1113, 1145
FaqI GGGAC 2 cut(s) 159, 517
FatI CATG 8 cut(s) 225, 424, 556, 586, 664, 763, 940, 1294
FauI CCCGC 1 cut(s) 475
FblI GTMKAC 1 cut(s) 261
Fnu4HI GCNGC 4 cut(s) 343, 553, 1045, 1089
FokI GGATG 2 cut(s) 49, 1427
FriOI GRGCYC 1 cut(s) 1157
Fsp4HI GCNGC 4 cut(s) 343, 553, 1045, 1089
FspBI CTAG 1 cut(s) 1235
GlaI GCGC 1 cut(s) 613
GluI GCNGC 4 cut(s) 343, 553, 1045, 1089
HaeII RGCGCY 1 cut(s) 615
HaeIII GGCC 7 cut(s) 110, 345, 371, 762, 821, 870, 971
HapII CCGG 3 cut(s) 171, 677, 824
HgaI GACGC 1 cut(s) 109
HhaI GCGC 1 cut(s) 614
Hin1I GRCGYC 2 cut(s) 120, 612
Hin1II CATG 8 cut(s) 229, 428, 560, 590, 668, 767, 944, 1298
Hin6I GCGC 1 cut(s) 612
HinP1I GCGC 1 cut(s) 612
HincII GTYRAC 3 cut(s) 262, 379, 1262
HindII GTYRAC 3 cut(s) 262, 379, 1262
HinfI GANTC 7 cut(s) 409, 421, 673, 1039, 1173, 1323, 1365
HpaII CCGG 3 cut(s) 171, 677, 824
HphI GGTGA 2 cut(s) 346, 722
Hpy166II GTNNAC 5 cut(s) 168, 262, 379, 546, 1262
Hpy188III TCNNGA 5 cut(s) 33, 677, 1019, 1133, 1327
Hpy8I GTNNAC 5 cut(s) 168, 262, 379, 546, 1262
Hpy99I CGWCG 2 cut(s) 266, 409
HpyAV CCTTC 3 cut(s) 15, 1072, 1118
HpyCH4III ACNGT 3 cut(s) 314, 334, 851
HpyCH4IV ACGT 1 cut(s) 642
HpyF10VI GCNNNNNNNGC 4 cut(s) 348, 609, 915, 1097
HpyF3I CTNAG 7 cut(s) 87, 96, 198, 215, 444, 1020, 1389
HpySE526I ACGT 1 cut(s) 642
Hsp92I GRCGYC 2 cut(s) 120, 612
Hsp92II CATG 8 cut(s) 229, 428, 560, 590, 668, 767, 944, 1298
HspAI GCGC 1 cut(s) 612
KasI GGCGCC 1 cut(s) 611
Kpn2I TCCGGA 1 cut(s) 676
Kzo9I GATC 5 cut(s) 189, 736, 1255, 1329, 1343
Lsp1109I GCAGC 2 cut(s) 539, 1075
LweI GCATC 3 cut(s) 261, 1387, 1405
MaeI CTAG 1 cut(s) 1235
MaeII ACGT 1 cut(s) 642
MaeIII GTNAC 3 cut(s) 961, 1009, 1249
MalI GATC 5 cut(s) 191, 738, 1257, 1331, 1345
MboI GATC 5 cut(s) 189, 736, 1255, 1329, 1343
MboII GAAGA 4 cut(s) 280, 481, 1279, 1406
MflI RGATCY 3 cut(s) 189, 736, 1343
MhlI GDGCHC 1 cut(s) 1157
MlsI TGGCCA 2 cut(s) 762, 971
MluCI AATT 6 cut(s) 44, 496, 635, 807, 815, 1048
MluNI TGGCCA 2 cut(s) 762, 971
Mly113I GGCGCC 1 cut(s) 612
MlyI GAGTC 3 cut(s) 1033, 1167, 1332
MmeI TCCRAC 4 cut(s) 82, 278, 421, 611
Mox20I TGGCCA 2 cut(s) 762, 971
Mph1103I ATGCAT 1 cut(s) 1144
MroI TCCGGA 1 cut(s) 676
MroXI GAANNNNTTC 1 cut(s) 1128
MscI TGGCCA 2 cut(s) 762, 971
MseI TTAA 4 cut(s) 26, 639, 1056, 1229
MslI CAYNNNNRTG 1 cut(s) 357
Msp20I TGGCCA 2 cut(s) 762, 971
MspI CCGG 3 cut(s) 171, 677, 824
MspR9I CCNGG 2 cut(s) 824, 825
MwoI GCNNNNNNNGC 4 cut(s) 348, 609, 915, 1097
NarI GGCGCC 1 cut(s) 612
NciI CCSGG 2 cut(s) 824, 825
NdeII GATC 5 cut(s) 189, 736, 1255, 1329, 1343
NlaIII CATG 8 cut(s) 229, 428, 560, 590, 668, 767, 944, 1298
NlaIV GGNNCC 6 cut(s) 175, 613, 681, 754, 822, 1345
NmuCI GTSAC 2 cut(s) 961, 1249
NsiI ATGCAT 1 cut(s) 1144
NspI RCATGY 1 cut(s) 1298
PceI AGGCCT 1 cut(s) 870
PciI ACATGT 1 cut(s) 1294
PdmI GAANNNNTTC 1 cut(s) 1128
PfeI GAWTC 4 cut(s) 409, 421, 673, 1365
PinAI ACCGGT 1 cut(s) 170
PkrI GCNGC 4 cut(s) 344, 554, 1046, 1090
PleI GAGTC 3 cut(s) 1033, 1167, 1331
PluTI GGCGCC 1 cut(s) 615
PpsI GAGTC 3 cut(s) 1033, 1167, 1331
PscI ACATGT 1 cut(s) 1294
PshBI ATTAAT 1 cut(s) 1229
PsiI TTATAA 1 cut(s) 846
PspN4I GGNNCC 6 cut(s) 175, 613, 681, 754, 822, 1345
PspPI GGNCC 4 cut(s) 173, 679, 820, 889
PstI CTGCAG 1 cut(s) 1093
PstNI CAGNNNCTG 2 cut(s) 280, 451
PsuI RGATCY 3 cut(s) 189, 736, 1343
RsaI GTAC 4 cut(s) 167, 212, 545, 951
RsaNI GTAC 4 cut(s) 166, 211, 544, 950
RseI CAYNNNNRTG 1 cut(s) 357
SalI GTCGAC 1 cut(s) 260
SaqAI TTAA 4 cut(s) 26, 639, 1056, 1229
SatI GCNGC 4 cut(s) 343, 553, 1045, 1089
Sau3AI GATC 5 cut(s) 189, 736, 1255, 1329, 1343
Sau96I GGNCC 4 cut(s) 173, 679, 820, 889
SchI GAGTC 3 cut(s) 1033, 1167, 1332
ScrFI CCNGG 2 cut(s) 824, 825
SduI GDGCHC 1 cut(s) 1157
SfaNI GCATC 3 cut(s) 261, 1387, 1405
SfcI CTRYAG 1 cut(s) 1089
SfoI GGCGCC 1 cut(s) 613
SinI GGWCC 3 cut(s) 173, 679, 889
SmaI CCCGGG 1 cut(s) 825
SmiMI CAYNNNNRTG 1 cut(s) 357
SmlI CTYRAG 1 cut(s) 476
SmoI CTYRAG 1 cut(s) 476
Sse9I AATT 6 cut(s) 44, 496, 635, 807, 815, 1048
SseBI AGGCCT 1 cut(s) 870
SsiI CCGC 4 cut(s) 161, 342, 468, 1045
SspDI GGCGCC 1 cut(s) 611
SspI AATATT 1 cut(s) 256
SspMI CTAG 1 cut(s) 1235
StuI AGGCCT 1 cut(s) 870
StyD4I CCNGG 2 cut(s) 822, 823
StyI CCWWGG 3 cut(s) 972, 997, 1278
TaaI ACNGT 3 cut(s) 314, 334, 851
TaiI ACGT 1 cut(s) 645
TaqI TCGA 5 cut(s) 147, 261, 285, 404, 744
TasI AATT 6 cut(s) 44, 496, 635, 807, 815, 1048
TatI WGTACW 1 cut(s) 543
TauI GCSGC 2 cut(s) 345, 1047
TfiI GAWTC 4 cut(s) 409, 421, 673, 1365
Tru1I TTAA 4 cut(s) 26, 639, 1056, 1229
Tru9I TTAA 4 cut(s) 26, 639, 1056, 1229
TscAI CASTG 4 cut(s) 353, 456, 622, 856
TseFI GTSAC 2 cut(s) 961, 1249
TseI GCWGC 2 cut(s) 552, 1088
Tsp45I GTSAC 2 cut(s) 961, 1249
TspDTI ATGAA 5 cut(s) 650, 686, 865, 1212, 1325
TspGWI ACGGA 1 cut(s) 521
TspMI CCCGGG 1 cut(s) 823
TspRI CASTG 4 cut(s) 353, 456, 622, 856
VpaK11BI GGWCC 3 cut(s) 173, 679, 889
VspI ATTAAT 1 cut(s) 1229
XapI RAATTY 1 cut(s) 635
XceI RCATGY 1 cut(s) 1298
XcmI CCANNNNNNNNNTGG 1 cut(s) 796
XmaI CCCGGG 1 cut(s) 823
XmiI GTMKAC 1 cut(s) 261
XmnI GAANNNNTTC 1 cut(s) 1128
XspI CTAG 1 cut(s) 1235
Zsp2I ATGCAT 1 cut(s) 1144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.