Rw3G009180

Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0009236
Physical Location & Seq
Reverse (-)
8756496 .. 8760463
3968 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G009180.1

Sequence Viewer

Length: 1380 bp
ATGCAGGGATTTATAGGGAGGAGGGTGGCTCAGACCGAGGATGCCGACAATGGCCTCCATATTCCCAAAATCCCCCCGTTCGACTACTCTCCTCCGCCGTACACCGGCCCCACCGCCGAGGAGATCTTGGCACAGAGGAAAGAGTACCTCAGCCCCTCCATGTTCTACTTCTACAACAAACCCCTGAATATTGTCGACGGGAAGATGCAGTACCTGTTCGACGAGAATGGTCGGAGGTATTTGGACGGTTTCGGAGGGATTGCTACGGTGAGCTGCGGCCACTGCCACCCTGATGTGGTGGAGGCCATAGTGAACCAAACCAAGAGACTACAACACTCGACGATTCTTTACCTGAATCATGCAATCGGCGATTTCGCTCAGGCACTGGCTTCCAAGTTGCCCGCCAATCTCAAGGTAGTGTTCTTCACTAATTCAGGGACGGAAGCAAACGAGTTGGCGATGTTGATAGCGAGGTTGTACACTGGCTGCCATGACATAATCTCTTTGAGAAACGCATATCATGGCAATGCTGCTGGCACTATGGGCGCCACTGCCCAGGCTAACTGGAAATTTAACGTCATTCAGAGTGGAGTTCATCATGCCATGAATCCAGACCCATACAGAGGTGTATTTGGTTCAGATGGTGAGAAGTATGCCAAAGATGTCCAAGATCTCATTGACTTTGGAACTTCTGGCCATGTTGCTGCCTTTATATCTGAAGCCATACAGGGAGTAGGGGGAATTGTAGAATTGGCCCCAGGTTATTTGCCAGCCGTTTATAATAGTGTAAAAAAAGCAGGAGGCCTTTTCATCGCTGATGAGGTCCAGTCTGGTTTTGCTCGCACAGGAAGCAACTTCTGGGGTTTTGAGGGCCATGGTGTTGTGCCTGACATTGTGACAATGGCCAAGAGCATTGGAAATGGCATTCCCCTTGGTGCTGTGGTAACCACTCCTGAGGTTGCAGAGGTCTTGACCCGCCGCAATTACTTTAACACCTTTGGTGGCAATCCTGTATGCACAGCTGCAGGACTGGCTGTTCTGAAAGTGATAGAGAAAGAAGGACTTCAAGAAAATGCATTCGTTGTGGGCTCTTATCTGAAAGAAAGACTCACTGCCCTTAAGGATAAATATGAACTTATTGGGGATGTGAGGGGAAAAGGATTAATGCTTGGAGTTGAACTTGTGACTGACCGTCAACTGAAAACACCAGCCAAGGCTGAAACTATACATGTGATGGACAAAATGAAGGATTTGGGAGTCTTGATCGGCAAAGGTGGGTTCTATGGAAATGTTTTCAGAATCACACCTCCCTTGTGCTTCACTAAGAAAGATGCAGATTTTCTTGTGGATGCAATGGACTACACAATGTCTAAAGTTTGA

Protein Analysis

459

Amino Acids

49.81

Weight (kDa)

6.32

Isoelectric Point (pI)

29.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_3 PF00202 62 - 453 5.1e-95 Aminotransferase class-III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 780
AccB1I GGYRCC 1 cut(s) 545
AccI GTMKAC 1 cut(s) 195
AciI CCGC 6 cut(s) 95, 114, 276, 402, 976, 979
AcoI YGGCCR 3 cut(s) 277, 694, 903
AcsI RAATTY 1 cut(s) 569
AcuI CTGAAG 1 cut(s) 738
AcyI GRCGYC 1 cut(s) 546
AfaI GTAC 4 cut(s) 101, 146, 212, 479
AfiI CCNNNNNNNGG 3 cut(s) 104, 295, 623
AflII CTTAAG 1 cut(s) 1118
AflIII ACRYGT 1 cut(s) 1228
AgsI TTSAA 2 cut(s) 1067, 1178
AhdI GACNNNNNGTC 1 cut(s) 1191
AjnI CCWGG 2 cut(s) 555, 757
AloI GAACNNNNNNTCC 2 cut(s) 1020, 1052
AluBI AGCT 2 cut(s) 273, 1022
AluI AGCT 2 cut(s) 273, 1022
Alw26I GTCTC 1 cut(s) 319
AlwNI CAGNNNCTG 2 cut(s) 214, 385
AoxI GGCC 9 cut(s) 52, 106, 277, 303, 694, 753, 802, 871, 903
ApeKI GCWGC 5 cut(s) 273, 486, 530, 704, 1022
ApoI RAATTY 1 cut(s) 569
AseI ATTAAT 1 cut(s) 1163
Asp700I GAANNNNTTC 1 cut(s) 1062
AspLEI GCGC 1 cut(s) 548
AspS9I GGNCC 4 cut(s) 107, 754, 823, 871
AsuHPI GGTGA 2 cut(s) 280, 656
AvaII GGWCC 1 cut(s) 823
AxyI CCTNAGG 1 cut(s) 954
BalI TGGCCA 2 cut(s) 696, 905
BanI GGYRCC 1 cut(s) 545
BanII GRGCYC 1 cut(s) 1091
BarI GAAGNNNNNNTAC 2 cut(s) 194, 226
BbvCI CCTCAGC 1 cut(s) 149
BbvI GCAGC 5 cut(s) 260, 473, 517, 691, 1009
BccI CCATC 2 cut(s) 635, 1228
BceAI ACGGC 2 cut(s) 82, 758
BciT130I CCWGG 2 cut(s) 557, 759
BcoDI GTCTC 1 cut(s) 319
BfmI CTRYAG 1 cut(s) 1023
BfoI RGCGCY 1 cut(s) 549
BfrI CTTAAG 1 cut(s) 1118
BglII AGATCT 2 cut(s) 123, 670
BisI GCNGC 7 cut(s) 274, 277, 487, 531, 705, 979, 1023
BlsI GCNGC 7 cut(s) 275, 278, 488, 532, 706, 980, 1024
Bme1390I CCNGG 2 cut(s) 557, 759
Bme18I GGWCC 1 cut(s) 823
BmeRI GACNNNNNGTC 1 cut(s) 1191
BmgT120I GGNCC 4 cut(s) 107, 754, 823, 871
BmiI GGNNCC 3 cut(s) 109, 547, 756
BmrFI CCNGG 2 cut(s) 557, 759
BmsI GCATC 4 cut(s) 31, 195, 1321, 1339
BplI GAGNNNNNCTC 2 cut(s) 13, 45
Bpu10I CCTNAGC 2 cut(s) 149, 378
BpuEI CTTGAG 1 cut(s) 395
BsaHI GRCGYC 1 cut(s) 546
BsaJI CCNNGG 7 cut(s) 36, 117, 555, 757, 874, 931, 1212
Bsc4I CCNNNNNNNGG 3 cut(s) 104, 295, 623
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 5 cut(s) 390, 487, 569, 826, 1035
Bse21I CCTNAGG 1 cut(s) 954
Bse3DI GCAATG 2 cut(s) 532, 1359
BseBI CCWGG 2 cut(s) 557, 759
BseDI CCNNGG 7 cut(s) 36, 117, 555, 757, 874, 931, 1212
BseGI GGATG 3 cut(s) 46, 1150, 1354
BseLI CCNNNNNNNGG 3 cut(s) 104, 295, 623
BseMI GCAATG 2 cut(s) 532, 1359
BseMII CTCAG 4 cut(s) 44, 163, 392, 945
BseNI ACTGG 5 cut(s) 390, 487, 569, 826, 1035
BseRI GAGGAG 3 cut(s) 34, 81, 134
BseXI GCAGC 5 cut(s) 260, 473, 517, 691, 1009
BshFI GGCC 9 cut(s) 54, 108, 279, 305, 696, 755, 804, 873, 905
BshNI GGYRCC 1 cut(s) 545
BsiSI CCGG 1 cut(s) 105
BslFI GGGAC 1 cut(s) 451
BslI CCNNNNNNNGG 3 cut(s) 104, 295, 623
BsmAI GTCTC 1 cut(s) 319
BsmFI GGGAC 1 cut(s) 451
BsmI GAATGC 2 cut(s) 924, 1076
BsnI GGCC 9 cut(s) 54, 108, 279, 305, 696, 755, 804, 873, 905
Bsp1286I GDGCHC 1 cut(s) 1091
Bsp1407I TGTACA 1 cut(s) 477
Bsp143I GATC 3 cut(s) 123, 670, 1263
Bsp19I CCATGG 1 cut(s) 874
BspACI CCGC 6 cut(s) 95, 114, 276, 402, 976, 979
BspANI GGCC 9 cut(s) 54, 108, 279, 305, 696, 755, 804, 873, 905
BspCNI CTCAG 4 cut(s) 43, 162, 391, 946
BspLI GGNNCC 3 cut(s) 109, 547, 756
BspMAI CTGCAG 1 cut(s) 1027
BspT107I GGYRCC 1 cut(s) 545
BspTI CTTAAG 1 cut(s) 1118
BsrDI GCAATG 2 cut(s) 532, 1359
BsrFI RCCGGY 1 cut(s) 104
BsrGI TGTACA 1 cut(s) 477
BsrI ACTGG 5 cut(s) 390, 487, 569, 826, 1035
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 7 cut(s) 36, 117, 555, 757, 874, 931, 1212
BssMI GATC 3 cut(s) 123, 670, 1263
BssNI GRCGYC 1 cut(s) 546
BssT1I CCWWGG 3 cut(s) 874, 931, 1212
Bst2UI CCWGG 2 cut(s) 557, 759
Bst4CI ACNGT 3 cut(s) 248, 268, 1193
BstACI GRCGYC 1 cut(s) 546
BstAFI CTTAAG 1 cut(s) 1118
BstAUI TGTACA 1 cut(s) 477
BstC8I GCNNGC 4 cut(s) 402, 535, 771, 841
BstDEI CTNAG 5 cut(s) 30, 149, 378, 954, 1323
BstDSI CCRYGG 1 cut(s) 874
BstEII GGTNACC 1 cut(s) 943
BstF5I GGATG 3 cut(s) 46, 1150, 1354
BstH2I RGCGCY 1 cut(s) 549
BstHHI GCGC 1 cut(s) 548
BstKTI GATC 3 cut(s) 126, 673, 1266
BstMAI GTCTC 1 cut(s) 319
BstMBI GATC 3 cut(s) 123, 670, 1263
BstMWI GCNNNNNNNGC 4 cut(s) 282, 543, 849, 1031
BstNI CCWGG 2 cut(s) 557, 759
BstNSI RCATGY 1 cut(s) 1232
BstPI GGTNACC 1 cut(s) 943
BstSCI CCNGG 2 cut(s) 555, 757
BstSFI CTRYAG 1 cut(s) 1023
BstV1I GCAGC 5 cut(s) 260, 473, 517, 691, 1009
BstX2I RGATCY 2 cut(s) 123, 670
BstYI RGATCY 2 cut(s) 123, 670
Bsu36I CCTNAGG 1 cut(s) 954
BsuRI GGCC 9 cut(s) 54, 108, 279, 305, 696, 755, 804, 873, 905
BtgI CCRYGG 1 cut(s) 874
BtgZI GCGATG 2 cut(s) 473, 796
BtsCI GGATG 3 cut(s) 46, 1150, 1354
BtsI GCAGTG 3 cut(s) 280, 549, 1110
BtsIMutI CAGTG 5 cut(s) 280, 383, 480, 549, 1110
Cac8I GCNNGC 4 cut(s) 402, 535, 771, 841
CaiI CAGNNNCTG 2 cut(s) 214, 385
CfoI GCGC 1 cut(s) 548
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 4 cut(s) 107, 754, 823, 871
Csp6I GTAC 4 cut(s) 100, 145, 211, 478
CviAII CATG 9 cut(s) 160, 359, 491, 521, 599, 604, 698, 875, 1229
CviQI GTAC 4 cut(s) 100, 145, 211, 478
DdeI CTNAG 5 cut(s) 30, 149, 378, 954, 1323
DinI GGCGCC 1 cut(s) 547
DpnI GATC 3 cut(s) 125, 672, 1265
DpnII GATC 3 cut(s) 123, 670, 1263
DriI GACNNNNNGTC 1 cut(s) 1191
EaeI YGGCCR 3 cut(s) 277, 694, 903
Eam1105I GACNNNNNGTC 1 cut(s) 1191
EciI GGCGGA 1 cut(s) 84
Eco130I CCWWGG 3 cut(s) 874, 931, 1212
Eco147I AGGCCT 1 cut(s) 804
Eco24I GRGCYC 1 cut(s) 1091
Eco47I GGWCC 1 cut(s) 823
Eco57I CTGAAG 1 cut(s) 738
Eco81I CCTNAGG 1 cut(s) 954
Eco91I GGTNACC 1 cut(s) 943
EcoO65I GGTNACC 1 cut(s) 943
EcoRII CCWGG 2 cut(s) 555, 757
EcoT14I CCWWGG 3 cut(s) 874, 931, 1212
EcoT22I ATGCAT 1 cut(s) 1078
EcoT38I GRGCYC 1 cut(s) 1091
EgeI GGCGCC 1 cut(s) 547
EheI GGCGCC 1 cut(s) 547
ErhI CCWWGG 3 cut(s) 874, 931, 1212
FaeI CATG 9 cut(s) 163, 362, 494, 524, 602, 607, 701, 878, 1232
FalI AAGNNNNNCTT 2 cut(s) 1047, 1079
FaqI GGGAC 1 cut(s) 451
FatI CATG 9 cut(s) 159, 358, 490, 520, 598, 603, 697, 874, 1228
FauI CCCGC 2 cut(s) 409, 983
FblI GTMKAC 1 cut(s) 195
Fnu4HI GCNGC 7 cut(s) 274, 277, 487, 531, 705, 979, 1023
FokI GGATG 3 cut(s) 53, 1157, 1361
FriOI GRGCYC 1 cut(s) 1091
Fsp4HI GCNGC 7 cut(s) 274, 277, 487, 531, 705, 979, 1023
GlaI GCGC 1 cut(s) 547
GluI GCNGC 7 cut(s) 274, 277, 487, 531, 705, 979, 1023
HaeII RGCGCY 1 cut(s) 549
HaeIII GGCC 9 cut(s) 54, 108, 279, 305, 696, 755, 804, 873, 905
HapII CCGG 1 cut(s) 105
HhaI GCGC 1 cut(s) 548
Hin1I GRCGYC 1 cut(s) 546
Hin1II CATG 9 cut(s) 163, 362, 494, 524, 602, 607, 701, 878, 1232
Hin6I GCGC 1 cut(s) 546
HinP1I GCGC 1 cut(s) 546
HincII GTYRAC 2 cut(s) 196, 1196
HindII GTYRAC 2 cut(s) 196, 1196
HinfI GANTC 6 cut(s) 343, 355, 607, 1107, 1257, 1299
HpaII CCGG 1 cut(s) 105
HphI GGTGA 2 cut(s) 280, 656
Hpy166II GTNNAC 5 cut(s) 102, 196, 313, 480, 1196
Hpy188I TCNGA 9 cut(s) 33, 234, 254, 585, 640, 718, 1041, 1098, 1298
Hpy188III TCNNGA 5 cut(s) 611, 953, 970, 1067, 1261
Hpy8I GTNNAC 5 cut(s) 102, 196, 313, 480, 1196
Hpy99I CGWCG 3 cut(s) 200, 224, 343
HpyAV CCTTC 2 cut(s) 1052, 1240
HpyCH4III ACNGT 3 cut(s) 248, 268, 1193
HpyCH4IV ACGT 1 cut(s) 576
HpyCH4V TGCA 9 cut(s) 4, 208, 362, 962, 1017, 1025, 1076, 1334, 1352
HpyF10VI GCNNNNNNNGC 4 cut(s) 282, 543, 849, 1031
HpyF3I CTNAG 5 cut(s) 30, 149, 378, 954, 1323
HpySE526I ACGT 1 cut(s) 576
Hsp92I GRCGYC 1 cut(s) 546
Hsp92II CATG 9 cut(s) 163, 362, 494, 524, 602, 607, 701, 878, 1232
HspAI GCGC 1 cut(s) 546
KasI GGCGCC 1 cut(s) 545
Kzo9I GATC 3 cut(s) 123, 670, 1263
Lsp1109I GCAGC 5 cut(s) 260, 473, 517, 691, 1009
LweI GCATC 4 cut(s) 31, 195, 1321, 1339
MaeII ACGT 1 cut(s) 576
MaeIII GTNAC 3 cut(s) 895, 943, 1183
MalI GATC 3 cut(s) 125, 672, 1265
MboI GATC 3 cut(s) 123, 670, 1263
MboII GAAGA 2 cut(s) 214, 415
MflI RGATCY 2 cut(s) 123, 670
MhlI GDGCHC 1 cut(s) 1091
MlsI TGGCCA 2 cut(s) 696, 905
MluCI AATT 5 cut(s) 430, 569, 741, 749, 982
MluNI TGGCCA 2 cut(s) 696, 905
Mly113I GGCGCC 1 cut(s) 546
MlyI GAGTC 2 cut(s) 1101, 1266
MmeI TCCRAC 1 cut(s) 212
Mox20I TGGCCA 2 cut(s) 696, 905
Mph1103I ATGCAT 1 cut(s) 1078
MroXI GAANNNNTTC 1 cut(s) 1062
MscI TGGCCA 2 cut(s) 696, 905
MseI TTAA 4 cut(s) 573, 990, 1119, 1163
MslI CAYNNNNRTG 2 cut(s) 291, 525
Msp20I TGGCCA 2 cut(s) 696, 905
MspA1I CMGCKG 1 cut(s) 1022
MspCI CTTAAG 1 cut(s) 1118
MspI CCGG 1 cut(s) 105
MspR9I CCNGG 2 cut(s) 557, 759
Mva1269I GAATGC 2 cut(s) 924, 1076
MvaI CCWGG 2 cut(s) 557, 759
MwoI GCNNNNNNNGC 4 cut(s) 282, 543, 849, 1031
NarI GGCGCC 1 cut(s) 546
NcoI CCATGG 1 cut(s) 874
NdeII GATC 3 cut(s) 123, 670, 1263
NlaIII CATG 9 cut(s) 163, 362, 494, 524, 602, 607, 701, 878, 1232
NlaIV GGNNCC 3 cut(s) 109, 547, 756
NmeAIII GCCGAG 1 cut(s) 142
NmuCI GTSAC 2 cut(s) 895, 1183
NsiI ATGCAT 1 cut(s) 1078
NspI RCATGY 1 cut(s) 1232
PceI AGGCCT 1 cut(s) 804
PciI ACATGT 1 cut(s) 1228
PctI GAATGC 2 cut(s) 924, 1076
PdmI GAANNNNTTC 1 cut(s) 1062
PfeI GAWTC 4 cut(s) 343, 355, 607, 1299
PkrI GCNGC 7 cut(s) 275, 278, 488, 532, 706, 980, 1024
PleI GAGTC 2 cut(s) 1101, 1265
PluTI GGCGCC 1 cut(s) 549
PpsI GAGTC 2 cut(s) 1101, 1265
PscI ACATGT 1 cut(s) 1228
PshBI ATTAAT 1 cut(s) 1163
PsiI TTATAA 1 cut(s) 780
Psp6I CCWGG 2 cut(s) 555, 757
PspEI GGTNACC 1 cut(s) 943
PspGI CCWGG 2 cut(s) 555, 757
PspN4I GGNNCC 3 cut(s) 109, 547, 756
PspPI GGNCC 4 cut(s) 107, 754, 823, 871
PstI CTGCAG 1 cut(s) 1027
PstNI CAGNNNCTG 2 cut(s) 214, 385
PsuI RGATCY 2 cut(s) 123, 670
PvuII CAGCTG 1 cut(s) 1022
RsaI GTAC 4 cut(s) 101, 146, 212, 479
RsaNI GTAC 4 cut(s) 100, 145, 211, 478
RseI CAYNNNNRTG 2 cut(s) 291, 525
SalI GTCGAC 1 cut(s) 194
SaqAI TTAA 4 cut(s) 573, 990, 1119, 1163
SatI GCNGC 7 cut(s) 274, 277, 487, 531, 705, 979, 1023
Sau3AI GATC 3 cut(s) 123, 670, 1263
Sau96I GGNCC 4 cut(s) 107, 754, 823, 871
SchI GAGTC 2 cut(s) 1101, 1266
ScrFI CCNGG 2 cut(s) 557, 759
SduI GDGCHC 1 cut(s) 1091
SfaNI GCATC 4 cut(s) 31, 195, 1321, 1339
SfcI CTRYAG 1 cut(s) 1023
SfoI GGCGCC 1 cut(s) 547
SinI GGWCC 1 cut(s) 823
SmiMI CAYNNNNRTG 2 cut(s) 291, 525
SmlI CTYRAG 2 cut(s) 410, 1118
SmoI CTYRAG 2 cut(s) 410, 1118
Sse9I AATT 5 cut(s) 430, 569, 741, 749, 982
SseBI AGGCCT 1 cut(s) 804
SsiI CCGC 6 cut(s) 95, 114, 276, 402, 976, 979
SspDI GGCGCC 1 cut(s) 545
SspI AATATT 1 cut(s) 190
StuI AGGCCT 1 cut(s) 804
StyD4I CCNGG 2 cut(s) 555, 757
StyI CCWWGG 3 cut(s) 874, 931, 1212
TaaI ACNGT 3 cut(s) 248, 268, 1193
TaiI ACGT 1 cut(s) 579
TaqI TCGA 4 cut(s) 81, 195, 219, 338
TaqII GACCGA 1 cut(s) 50
TasI AATT 5 cut(s) 430, 569, 741, 749, 982
TatI WGTACW 1 cut(s) 477
TauI GCSGC 2 cut(s) 279, 981
TfiI GAWTC 4 cut(s) 343, 355, 607, 1299
Tru1I TTAA 4 cut(s) 573, 990, 1119, 1163
Tru9I TTAA 4 cut(s) 573, 990, 1119, 1163
TscAI CASTG 5 cut(s) 287, 390, 487, 556, 1117
TseFI GTSAC 2 cut(s) 895, 1183
TseI GCWGC 5 cut(s) 273, 486, 530, 704, 1022
Tsp45I GTSAC 2 cut(s) 895, 1183
TspDTI ATGAA 5 cut(s) 584, 620, 799, 1146, 1259
TspGWI ACGGA 1 cut(s) 455
TspRI CASTG 5 cut(s) 287, 390, 487, 556, 1117
Vha464I CTTAAG 1 cut(s) 1118
VpaK11BI GGWCC 1 cut(s) 823
VspI ATTAAT 1 cut(s) 1163
XapI RAATTY 1 cut(s) 569
XceI RCATGY 1 cut(s) 1232
XmiI GTMKAC 1 cut(s) 195
XmnI GAANNNNTTC 1 cut(s) 1062
Zsp2I ATGCAT 1 cut(s) 1078
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.