FvH4_6g11360

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0015650
Physical Location & Seq
Forward (+)
6783244 .. 6785093
1850 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g11360.t1

Sequence Viewer

Length: 1074 bp
ATGATGAAGTGTGTGCAGAGCTGGCCTGAGCCGATTGTTCGAGTCCAGTCCCTCGCCCAAAGCGGGATGACAAAAATCCCGGAGCACTACATCAAGCCCCCTTCCAAACGACCCTCGAATGTTGACAATACCACCGTCAACATCCCAGTCATCGACATGCAGTCCCTGACCGGAGACGACCAGGGCCTGCGTGAGACCACGCTGCAGCTGGTCTCGAACGCCTGTAGGGAGTGGGGGTTCTTCCAGGTGGTGAACCATGGTGTGAGCCACGAGCTGATGAAGCGGGCGAGGGAGACGTGGAGGGAGTTCTTCGAGCAGCCGCTGGGGGTGAAGCAGGAGTACTCCAACGACCCGAGCACCTATGAAGGGTACGGAAGTCGGCTGGGAGTGGAGAAGGGGGCGATTCTGGATTGGAGTGACTATTACTTTCTTCATTACTTGCCTCCGGCATTGAGGAAGCCCCACAAATGGCCTGCTCTCCCATCTTCATGCAGGGATTTGATCGAAGAATACGGTGAGGAAGCTACTAAACTATGTGGAAGACTGGTGAAGATGCTGTCACTAAACCTTGGGCTAGGGGAAGACTACCTCCCCAATGCTTTCGGAGGAGAGAACGTTGGGGCATGCTTAAGGGTCAATTTCTATCCAAGATGTCCCCAACCAGACCTAACCCTTGGAATCTCGCCACACTCGGACCCCGGCGGGATGACCCTTCTCCTGCCCGACGAGGACGTTGCCGGACTTCAAGTTCGTAAAGGTGACAATTGGGTGACTGTGAAACCCGTTCCAAATGCCTTCATTGTGAACATGGGAGACCAAATGCAGGTTCTGAGCAACGCCATATACAAAAGCGTAGAGCACAGAGTGATTGTGAATTCAGTCAAGGATCGGGTTTCTCTGGCCATGTTCTATAACCCCAAGAGCGATCTGCTGATCGAACCGGCCAAGGCTCTTGTCACGATGGACCGGCCGGCTCTCTACCCGGCGATGACGTTCGATGAGTACCGCATTTACATTAGGACCAAGGGGCTCTGTGGCAAGGCACAAGTTGAATCGTTGAAAACTCATGAATAA

Protein Analysis

358

Amino Acids

40.24

Weight (kDa)

6.27

Isoelectric Point (pI)

45.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 48 - 159 3e-31 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 208 - 306 2.2e-33 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015650)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g11360
malus_domestica MD04G1154400.v1.1 MD12G1168100.v1.1
prunus_persica Prupe.6G278400_v2.0.a1
pyrus_communis pycom04g13930 pycom12g15960
rosa_chinensis RchiOBHm_Chr3g0462961
rosa_laevigata RLG00000024821
rosa_multiflora Rmu_sc0000766.1_g000023
rosa_roxburghii Rroxscaffold_6G00417080
rosa_rugosa Rorug03G0060300
rosa_samantha Rh3AG118500 Rh3BG123500 Rh3CG125200 Rh3DG124200
rosa_wichuraiana Rw3G010050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 814
AciI CCGC 5 cut(s) 63, 283, 320, 702, 1006
AclI AACGTT 1 cut(s) 615
AclWI GGATC 1 cut(s) 894
AcoI YGGCCR 3 cut(s) 900, 942, 968
AcsI RAATTY 1 cut(s) 874
AdeI CACNNNGTG 1 cut(s) 865
AfaI GTAC 3 cut(s) 341, 371, 1004
AfiI CCNNNNNNNGG 4 cut(s) 63, 366, 468, 823
AflII CTTAAG 1 cut(s) 628
AgsI TTSAA 3 cut(s) 746, 1052, 1060
AhdI GACNNNNNGTC 1 cut(s) 160
AjiI CACGTC 1 cut(s) 297
AjnI CCWGG 2 cut(s) 180, 243
AjuI GAANNNNNNNTTGG 2 cut(s) 810, 842
AloI GAACNNNNNNTCC 4 cut(s) 221, 253, 732, 764
AluBI AGCT 4 cut(s) 21, 208, 274, 524
AluI AGCT 4 cut(s) 21, 208, 274, 524
Alw21I GWGCWC 3 cut(s) 87, 359, 861
Alw26I GTCTC 5 cut(s) 168, 188, 217, 287, 807
AlwI GGATC 1 cut(s) 894
AlwNI CAGNNNCTG 4 cut(s) 166, 187, 322, 829
Ama87I CYCGRG 1 cut(s) 352
AoxI GGCC 6 cut(s) 23, 184, 470, 900, 942, 968
ApeKI GCWGC 3 cut(s) 202, 205, 316
ApoI RAATTY 1 cut(s) 874
ArsI GACNNNNNNTTYG 2 cut(s) 732, 764
AspS9I GGNCC 4 cut(s) 184, 694, 964, 1020
AsuC2I CCSGG 3 cut(s) 80, 699, 983
AsuHPI GGTGA 6 cut(s) 262, 340, 527, 559, 770, 781
AvaI CYCGRG 1 cut(s) 352
AvaII GGWCC 3 cut(s) 694, 964, 1020
BaeI ACNNNNGTAYC 2 cut(s) 361, 394
BalI TGGCCA 1 cut(s) 902
BanII GRGCYC 1 cut(s) 1032
BarI GAAGNNNNNNTAC 2 cut(s) 323, 355
BauI CACGAG 1 cut(s) 269
BbsI GAAGAC 2 cut(s) 547, 588
Bbv12I GWGCWC 3 cut(s) 87, 359, 861
BbvI GCAGC 3 cut(s) 189, 217, 328
BccI CCATC 2 cut(s) 490, 955
BcgI CGANNNNNNTGC 2 cut(s) 716, 750
BciT130I CCWGG 2 cut(s) 182, 245
BcnI CCSGG 3 cut(s) 80, 699, 983
BcoDI GTCTC 5 cut(s) 168, 188, 217, 287, 807
BfaI CTAG 1 cut(s) 575
BfmI CTRYAG 2 cut(s) 203, 223
BfrI CTTAAG 1 cut(s) 628
BfuAI ACCTGC 1 cut(s) 814
BisI GCNGC 4 cut(s) 203, 206, 317, 320
BlsI GCNGC 4 cut(s) 204, 207, 318, 321
BmcAI AGTACT 1 cut(s) 341
Bme1390I CCNGG 5 cut(s) 80, 182, 245, 699, 983
Bme18I GGWCC 3 cut(s) 694, 964, 1020
BmeRI GACNNNNNGTC 1 cut(s) 160
BmeT110I CYCGRG 1 cut(s) 352
BmgBI CACGTC 1 cut(s) 297
BmgT120I GGNCC 4 cut(s) 184, 694, 964, 1020
BmiI GGNNCC 1 cut(s) 696
BmrFI CCNGG 5 cut(s) 80, 182, 245, 699, 983
BmrI ACTGGG 1 cut(s) 140
BmsI GCATC 1 cut(s) 543
BmuI ACTGGG 1 cut(s) 140
BpiI GAAGAC 2 cut(s) 547, 588
Bpu10I CCTNAGC 1 cut(s) 27
BpuMI CCSGG 3 cut(s) 80, 699, 983
BsaI GGTCTC 3 cut(s) 188, 217, 807
BsaJI CCNNGG 7 cut(s) 181, 256, 568, 673, 697, 945, 1023
BsaWI WCCGGW 1 cut(s) 170
BsaXI ACNNNNNCTCC 4 cut(s) 221, 251, 600, 630
Bsc4I CCNNNNNNNGG 4 cut(s) 63, 366, 468, 823
Bse118I RCCGGY 3 cut(s) 940, 966, 970
Bse1I ACTGG 3 cut(s) 46, 146, 549
BseBI CCWGG 2 cut(s) 182, 245
BseDI CCNNGG 7 cut(s) 181, 256, 568, 673, 697, 945, 1023
BseGI GGATG 3 cut(s) 72, 141, 711
BseLI CCNNNNNNNGG 4 cut(s) 63, 366, 468, 823
BseMII CTCAG 2 cut(s) 18, 821
BseNI ACTGG 3 cut(s) 46, 146, 549
BseRI GAGGAG 1 cut(s) 621
BseX3I CGGCCG 1 cut(s) 968
BseXI GCAGC 3 cut(s) 189, 217, 328
BseYI CCCAGC 2 cut(s) 322, 382
BsgI GTGCAG 1 cut(s) 35
Bsh1285I CGRYCG 1 cut(s) 971
BshFI GGCC 6 cut(s) 25, 186, 472, 902, 944, 970
BsiEI CGRYCG 1 cut(s) 971
BsiHKAI GWGCWC 3 cut(s) 87, 359, 861
BsiHKCI CYCGRG 1 cut(s) 352
BsiSI CCGG 9 cut(s) 80, 171, 446, 699, 738, 941, 967, 971, 983
BslFI GGGAC 3 cut(s) 34, 148, 639
BslI CCNNNNNNNGG 4 cut(s) 63, 366, 468, 823
BsmAI GTCTC 5 cut(s) 168, 188, 217, 287, 807
BsmBI CGTCTC 2 cut(s) 168, 287
BsmFI GGGAC 3 cut(s) 34, 148, 639
BsnI GGCC 6 cut(s) 25, 186, 472, 902, 944, 970
Bso31I GGTCTC 3 cut(s) 188, 217, 807
BsoBI CYCGRG 1 cut(s) 352
Bsp1286I GDGCHC 4 cut(s) 87, 359, 861, 1032
Bsp143I GATC 4 cut(s) 501, 886, 925, 933
Bsp19I CCATGG 1 cut(s) 256
BspACI CCGC 5 cut(s) 63, 283, 320, 702, 1006
BspANI GGCC 6 cut(s) 25, 186, 472, 902, 944, 970
BspCNI CTCAG 2 cut(s) 19, 822
BspHI TCATGA 1 cut(s) 1066
BspLI GGNNCC 1 cut(s) 696
BspMAI CTGCAG 1 cut(s) 207
BspMI ACCTGC 1 cut(s) 814
BspPI GGATC 1 cut(s) 894
BspTI CTTAAG 1 cut(s) 628
BspTNI GGTCTC 3 cut(s) 188, 217, 807
BsrFI RCCGGY 3 cut(s) 940, 966, 970
BsrI ACTGG 3 cut(s) 46, 146, 549
BssAI RCCGGY 3 cut(s) 940, 966, 970
BssECI CCNNGG 7 cut(s) 181, 256, 568, 673, 697, 945, 1023
BssMI GATC 4 cut(s) 501, 886, 925, 933
BssSI CACGAG 1 cut(s) 269
BssT1I CCWWGG 5 cut(s) 256, 568, 673, 945, 1023
Bst2BI CACGAG 1 cut(s) 269
Bst2UI CCWGG 2 cut(s) 182, 245
Bst4CI ACNGT 3 cut(s) 136, 515, 775
BstAFI CTTAAG 1 cut(s) 628
BstC8I GCNNGC 6 cut(s) 23, 188, 285, 474, 625, 972
BstDEI CTNAG 2 cut(s) 27, 830
BstDSI CCRYGG 1 cut(s) 256
BstF5I GGATG 3 cut(s) 72, 141, 711
BstKTI GATC 4 cut(s) 504, 889, 928, 936
BstMAI GTCTC 5 cut(s) 168, 188, 217, 287, 807
BstMBI GATC 4 cut(s) 501, 886, 925, 933
BstMCI CGRYCG 1 cut(s) 971
BstMWI GCNNNNNNNGC 2 cut(s) 22, 280
BstNI CCWGG 2 cut(s) 182, 245
BstNSI RCATGY 2 cut(s) 160, 627
BstSCI CCNGG 5 cut(s) 78, 180, 243, 697, 981
BstSFI CTRYAG 2 cut(s) 203, 223
BstV1I GCAGC 3 cut(s) 189, 217, 328
BstV2I GAAGAC 2 cut(s) 547, 588
BstZI CGGCCG 1 cut(s) 968
BsuRI GGCC 6 cut(s) 25, 186, 472, 902, 944, 970
BtgI CCRYGG 1 cut(s) 256
BtgZI GCGATG 1 cut(s) 1001
BtrI CACGTC 1 cut(s) 297
BtsCI GGATG 3 cut(s) 72, 141, 711
BveI ACCTGC 1 cut(s) 814
Cac8I GCNNGC 6 cut(s) 23, 188, 285, 474, 625, 972
CaiI CAGNNNCTG 4 cut(s) 166, 187, 322, 829
CciI TCATGA 1 cut(s) 1066
Cfr10I RCCGGY 3 cut(s) 940, 966, 970
Cfr13I GGNCC 4 cut(s) 184, 694, 964, 1020
Csp6I GTAC 3 cut(s) 340, 370, 1003
CviAII CATG 7 cut(s) 157, 257, 489, 624, 808, 904, 1067
CviQI GTAC 3 cut(s) 340, 370, 1003
DdeI CTNAG 2 cut(s) 27, 830
DpnI GATC 4 cut(s) 503, 888, 927, 935
DpnII GATC 4 cut(s) 501, 886, 925, 933
DraIII CACNNNGTG 1 cut(s) 865
DriI GACNNNNNGTC 1 cut(s) 160
EaeI YGGCCR 3 cut(s) 900, 942, 968
EagI CGGCCG 1 cut(s) 968
Eam1105I GACNNNNNGTC 1 cut(s) 160
EclXI CGGCCG 1 cut(s) 968
Eco130I CCWWGG 5 cut(s) 256, 568, 673, 945, 1023
Eco24I GRGCYC 1 cut(s) 1032
Eco31I GGTCTC 3 cut(s) 188, 217, 807
Eco47I GGWCC 3 cut(s) 694, 964, 1020
Eco52I CGGCCG 1 cut(s) 968
Eco88I CYCGRG 1 cut(s) 352
EcoO109I RGGNCCY 1 cut(s) 184
EcoRI GAATTC 1 cut(s) 874
EcoRII CCWGG 2 cut(s) 180, 243
EcoT14I CCWWGG 5 cut(s) 256, 568, 673, 945, 1023
EcoT38I GRGCYC 1 cut(s) 1032
ErhI CCWWGG 5 cut(s) 256, 568, 673, 945, 1023
Esp3I CGTCTC 2 cut(s) 168, 287
FaeI CATG 7 cut(s) 160, 260, 492, 627, 811, 907, 1070
FaqI GGGAC 3 cut(s) 34, 148, 639
FatI CATG 7 cut(s) 156, 256, 488, 623, 807, 903, 1066
FauI CCCGC 3 cut(s) 56, 276, 695
Fnu4HI GCNGC 4 cut(s) 203, 206, 317, 320
FokI GGATG 3 cut(s) 79, 128, 718
FriOI GRGCYC 1 cut(s) 1032
Fsp4HI GCNGC 4 cut(s) 203, 206, 317, 320
FspBI CTAG 1 cut(s) 575
GluI GCNGC 4 cut(s) 203, 206, 317, 320
GsaI CCCAGC 2 cut(s) 326, 386
HaeIII GGCC 6 cut(s) 25, 186, 472, 902, 944, 970
HapII CCGG 9 cut(s) 80, 171, 446, 699, 738, 941, 967, 971, 983
Hin1II CATG 7 cut(s) 160, 260, 492, 627, 811, 907, 1070
HincII GTYRAC 2 cut(s) 124, 139
HindII GTYRAC 2 cut(s) 124, 139
HinfI GANTC 4 cut(s) 42, 403, 678, 1052
HpaII CCGG 9 cut(s) 80, 171, 446, 699, 738, 941, 967, 971, 983
HphI GGTGA 6 cut(s) 262, 340, 527, 559, 770, 781
Hpy166II GTNNAC 4 cut(s) 124, 139, 253, 805
Hpy188I TCNGA 3 cut(s) 605, 694, 831
Hpy188III TCNNGA 4 cut(s) 214, 407, 958, 1067
Hpy8I GTNNAC 4 cut(s) 124, 139, 253, 805
Hpy99I CGWCG 1 cut(s) 728
HpyAV CCTTC 5 cut(s) 111, 359, 388, 722, 805
HpyCH4III ACNGT 3 cut(s) 136, 515, 775
HpyCH4IV ACGT 4 cut(s) 296, 615, 732, 992
HpyCH4V TGCA 5 cut(s) 16, 160, 205, 492, 823
HpyF10VI GCNNNNNNNGC 2 cut(s) 22, 280
HpyF3I CTNAG 2 cut(s) 27, 830
HpySE526I ACGT 4 cut(s) 296, 615, 732, 992
Hsp92II CATG 7 cut(s) 160, 260, 492, 627, 811, 907, 1070
KroI GCCGGC 1 cut(s) 970
KroNI GCCGGC 1 cut(s) 972
Kzo9I GATC 4 cut(s) 501, 886, 925, 933
LmnI GCTCC 1 cut(s) 82
Lsp1109I GCAGC 3 cut(s) 189, 217, 328
LweI GCATC 1 cut(s) 543
MaeI CTAG 1 cut(s) 575
MaeII ACGT 4 cut(s) 296, 615, 732, 992
MaeIII GTNAC 5 cut(s) 416, 558, 758, 769, 955
MalI GATC 4 cut(s) 503, 888, 927, 935
MboI GATC 4 cut(s) 501, 886, 925, 933
MboII GAAGA 8 cut(s) 232, 301, 422, 477, 518, 552, 562, 593
MfeI CAATTG 1 cut(s) 763
MhlI GDGCHC 4 cut(s) 87, 359, 861, 1032
MlsI TGGCCA 1 cut(s) 902
MluCI AATT 3 cut(s) 637, 763, 874
MluNI TGGCCA 1 cut(s) 902
MlyI GAGTC 1 cut(s) 51
MmeI TCCRAC 1 cut(s) 369
Mox20I TGGCCA 1 cut(s) 902
MroNI GCCGGC 1 cut(s) 970
MscI TGGCCA 1 cut(s) 902
MseI TTAA 1 cut(s) 629
MslI CAYNNNNRTG 2 cut(s) 155, 487
Msp20I TGGCCA 1 cut(s) 902
MspA1I CMGCKG 2 cut(s) 208, 322
MspCI CTTAAG 1 cut(s) 628
MspI CCGG 9 cut(s) 80, 171, 446, 699, 738, 941, 967, 971, 983
MspR9I CCNGG 5 cut(s) 80, 182, 245, 699, 983
MunI CAATTG 1 cut(s) 763
MvaI CCWGG 2 cut(s) 182, 245
MwoI GCNNNNNNNGC 2 cut(s) 22, 280
NaeI GCCGGC 1 cut(s) 972
NciI CCSGG 3 cut(s) 80, 699, 983
NcoI CCATGG 1 cut(s) 256
NdeII GATC 4 cut(s) 501, 886, 925, 933
NgoMIV GCCGGC 1 cut(s) 970
NlaIII CATG 7 cut(s) 160, 260, 492, 627, 811, 907, 1070
NlaIV GGNNCC 1 cut(s) 696
NmuCI GTSAC 5 cut(s) 416, 558, 758, 769, 955
NspI RCATGY 2 cut(s) 160, 627
PaeI GCATGC 1 cut(s) 627
PagI TCATGA 1 cut(s) 1066
PdiI GCCGGC 1 cut(s) 972
PfeI GAWTC 3 cut(s) 403, 678, 1052
PfoI TCCNGGA 1 cut(s) 78
PkrI GCNGC 4 cut(s) 204, 207, 318, 321
PleI GAGTC 1 cut(s) 50
PpsI GAGTC 1 cut(s) 50
Psp1406I AACGTT 1 cut(s) 615
Psp6I CCWGG 2 cut(s) 180, 243
PspFI CCCAGC 2 cut(s) 322, 382
PspGI CCWGG 2 cut(s) 180, 243
PspN4I GGNNCC 1 cut(s) 696
PspPI GGNCC 4 cut(s) 184, 694, 964, 1020
PstI CTGCAG 1 cut(s) 207
PstNI CAGNNNCTG 4 cut(s) 166, 187, 322, 829
PvuII CAGCTG 1 cut(s) 208
RsaI GTAC 3 cut(s) 341, 371, 1004
RsaNI GTAC 3 cut(s) 340, 370, 1003
RseI CAYNNNNRTG 2 cut(s) 155, 487
SaqAI TTAA 1 cut(s) 629
SatI GCNGC 4 cut(s) 203, 206, 317, 320
Sau3AI GATC 4 cut(s) 501, 886, 925, 933
Sau96I GGNCC 4 cut(s) 184, 694, 964, 1020
ScaI AGTACT 1 cut(s) 341
SchI GAGTC 1 cut(s) 51
ScrFI CCNGG 5 cut(s) 80, 182, 245, 699, 983
SduI GDGCHC 4 cut(s) 87, 359, 861, 1032
SfaNI GCATC 1 cut(s) 543
SfcI CTRYAG 2 cut(s) 203, 223
SinI GGWCC 3 cut(s) 694, 964, 1020
SmiMI CAYNNNNRTG 2 cut(s) 155, 487
SmlI CTYRAG 1 cut(s) 628
SmoI CTYRAG 1 cut(s) 628
SphI GCATGC 1 cut(s) 627
Sse9I AATT 3 cut(s) 637, 763, 874
SsiI CCGC 5 cut(s) 63, 283, 320, 702, 1006
SspMI CTAG 1 cut(s) 575
StyD4I CCNGG 5 cut(s) 78, 180, 243, 697, 981
StyI CCWWGG 5 cut(s) 256, 568, 673, 945, 1023
TaaI ACNGT 3 cut(s) 136, 515, 775
TaiI ACGT 4 cut(s) 299, 618, 735, 995
TaqI TCGA 8 cut(s) 40, 116, 153, 215, 312, 504, 936, 996
TasI AATT 3 cut(s) 637, 763, 874
TatI WGTACW 1 cut(s) 339
TauI GCSGC 1 cut(s) 322
TfiI GAWTC 3 cut(s) 403, 678, 1052
Tru1I TTAA 1 cut(s) 629
Tru9I TTAA 1 cut(s) 629
TseFI GTSAC 5 cut(s) 416, 558, 758, 769, 955
TseI GCWGC 3 cut(s) 202, 205, 316
Tsp45I GTSAC 5 cut(s) 416, 558, 758, 769, 955
TspDTI ATGAA 6 cut(s) 20, 293, 378, 422, 477, 787
TspGWI ACGGA 1 cut(s) 387
Vha464I CTTAAG 1 cut(s) 628
VpaK11BI GGWCC 3 cut(s) 694, 964, 1020
XapI RAATTY 1 cut(s) 874
XceI RCATGY 2 cut(s) 160, 627
XcmI CCANNNNNNNNNTGG 1 cut(s) 205
XspI CTAG 1 cut(s) 575
ZrmI AGTACT 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.