Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0015650

Physical Location & Seq

Genomic Coordinates Reverse (-)

24219397 .. 24222018

Gene Structure 2622 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1154400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1089 bp

ATGAGTTGCTTGCAGTCTTGGCCTGAACCTATCGTGAGAGTCCAGTCCTTAGCAGAAAGCGGCATAACCGCTATCCCTGACCTCTACATCAAACCACCATCCAAACGACCATCACCACCAAACACCAACAACCACCGTGATGCTGATGTCAACATCCCGATCATTGACCTCCAAAACCTTTTGAGCGACAACCGGAGCCTCCGCGAGGCAACGATGCGTTCAATCTCGAGCGCCTGCAGGGAGTGGGGTTTTTTTCAGGTGGTAAACCACGGGGTGAGCCACGAGCTGATGAAGCGGGCGAGGGACACATGGCGCGAGTTCTTCAGCCTGCCTCTGGAGGTGAAGCAGGAGTACTCCAACAAGCCGAGTACGTACGAAGGGTACGGAAGTCGGCTGGGAGTTGAGAAGGGGGCTATTCTGGATTGGAGTGATTATTACTTTCTTCATTACATGCCTCCTCAGATCAGAAACCACAACAAATGGCCAGCTGTCCCAACTTCTTGCAGGAAATTGATTGAAGAATACAGCGAAGAAACGATTAAACTGTGTGGGATTCTGATGAAGGTGTTGTCTCTAAACCTTGGCCTTGGGGAAGACCAACTTCTCGATGCTTTCGGAGGAGAAGAAAATATTGGGGCATGTTTACGGGTCAATTTCTACCCAAAATGCCCTCAACCAGATCTAACCCTTGGTCTCTCAGAACACTCTGACCCAGGTGGCCTGACCCTTCTCTTACCTGATGAAAATGTTGCTGGACTTCAAGTTCGTAAAGGTGAAAATTGGGTGACTGTGAAGCCTGTTCCTAATGCCTTCATCGTGAACGTCGGAGATCAAACTCAGGTGCTGAGCAATGCAATTTACAAAAGTGTAGAACATAGAGTGATTGTGAATTCAGTTGAAGACAGAGTTTCACTGGCCTTGTTTTACAACCCAAAAAGTGATTTGATGATTGAACCTGCCAAAGCTCTTGTAACAAATGTTCGACCTGCCCTTTACTCGCCTATGACATTTGATGAGTATCGGCTTTATATTAGGATGAAGGGGCCTTGTGGAAAGGCACAAGTTGAATCTTTAAAATCCTATGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0009966 GO:0010646 GO:0016787 GO:0016810 GO:0023051 GO:0044424 GO:0044444 GO:0044464 GO:0048583 GO:0050789 GO:0050794 GO:0065007 GO:0120091 GO:2000022

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

363

Amino Acids

40.86

Weight (kDa)

6.04

Isoelectric Point (pI)

50.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	52 - 163	8.7e-34	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	213 - 311	2.6e-33	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015650)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g11360
malus_domestica	MD04G1154400.v1.1 MD12G1168100.v1.1
prunus_persica	Prupe.6G278400_v2.0.a1
pyrus_communis	pycom04g13930 pycom12g15960
rosa_chinensis	RchiOBHm_Chr3g0462961
rosa_laevigata	RLG00000024821
rosa_multiflora	Rmu_sc0000766.1_g000023
rosa_roxburghii	Rroxscaffold_6G00417080
rosa_rugosa	Rorug03G0060300
rosa_samantha	Rh3AG118500 Rh3BG123500 Rh3CG125200 Rh3DG124200
rosa_wichuraiana	Rw3G010050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	964, 994
AccII	CGCG	2 cut(s)	204, 315
AciI	CCGC	4 cut(s)	60, 69, 202, 295
AcoI	YGGCCR	1 cut(s)	482
AcsI	RAATTY	1 cut(s)	889
AcuI	CTGAAG	1 cut(s)	307
AdeI	CACNNNGTG	1 cut(s)	274
AfaI	GTAC	4 cut(s)	353, 370, 374, 383
AfiI	CCNNNNNNNGG	2 cut(s)	205, 334
AgsI	TTSAA	6 cut(s)	222, 518, 761, 899, 953, 1067
AjnI	CCWGG	1 cut(s)	712
AjuI	GAANNNNNNNTTGG	2 cut(s)	615, 647
AloI	GAACNNNNNNTCC	2 cut(s)	747, 779
AluBI	AGCT	3 cut(s)	286, 488, 965
AluI	AGCT	3 cut(s)	286, 488, 965
Alw26I	GTCTC	2 cut(s)	576, 698
AlwNI	CAGNNNCTG	1 cut(s)	844
Ama87I	CYCGRG	1 cut(s)	226
AoxI	GGCC	6 cut(s)	20, 482, 583, 718, 915, 1043
ApoI	RAATTY	1 cut(s)	889
ArsI	GACNNNNNNTTYG	2 cut(s)	747, 779
AspLEI	GCGC	2 cut(s)	233, 315
AspS9I	GGNCC	1 cut(s)	1043
AsuHPI	GGTGA	5 cut(s)	105, 286, 352, 785, 796
AvaI	CYCGRG	1 cut(s)	226
BaeI	ACNNNNGTAYC	2 cut(s)	373, 406
BalI	TGGCCA	1 cut(s)	484
BarI	GAAGNNNNNNTAC	2 cut(s)	335, 367
BauI	CACGAG	1 cut(s)	281
BbsI	GAAGAC	2 cut(s)	600, 906
BccI	CCATC	2 cut(s)	106, 118
BciT130I	CCWGG	1 cut(s)	714
BcoDI	GTCTC	2 cut(s)	576, 698
BfmI	CTRYAG	1 cut(s)	235
BfoI	RGCGCY	1 cut(s)	234
BfuAI	ACCTGC	2 cut(s)	964, 994
BglII	AGATCT	1 cut(s)	679
BisI	GCNGC	1 cut(s)	61
BlpI	GCTNAGC	1 cut(s)	845
BlsI	GCNGC	1 cut(s)	62
BmcAI	AGTACT	1 cut(s)	353
Bme1390I	CCNGG	1 cut(s)	714
BmeT110I	CYCGRG	1 cut(s)	226
BmgT120I	GGNCC	1 cut(s)	1043
BmiI	GGNNCC	2 cut(s)	197, 1044
BmrFI	CCNGG	1 cut(s)	714
BmsI	GCATC	3 cut(s)	130, 204, 598
BpiI	GAAGAC	2 cut(s)	600, 906
BpmI	CTGGAG	1 cut(s)	356
Bpu10I	CCTNAGC	1 cut(s)	49
Bpu1102I	GCTNAGC	1 cut(s)	845
BsaAI	YACGTR	1 cut(s)	372
BsaBI	GATNNNNATC	1 cut(s)	1017
BsaI	GGTCTC	1 cut(s)	698
BsaJI	CCNNGG	5 cut(s)	268, 580, 586, 688, 712
BsaWI	WCCGGW	1 cut(s)	192
Bsc4I	CCNNNNNNNGG	2 cut(s)	205, 334
Bse1I	ACTGG	2 cut(s)	43, 918
Bse3DI	GCAATG	1 cut(s)	856
Bse8I	GATNNNNATC	1 cut(s)	1017
BseBI	CCWGG	1 cut(s)	714
BseDI	CCNNGG	5 cut(s)	268, 580, 586, 688, 712
BseGI	GGATG	3 cut(s)	98, 153, 1041
BseJI	GATNNNNATC	1 cut(s)	1017
BseLI	CCNNNNNNNGG	2 cut(s)	205, 334
BseMI	GCAATG	1 cut(s)	856
BseMII	CTCAG	4 cut(s)	473, 711, 836, 851
BseNI	ACTGG	2 cut(s)	43, 918
BseRI	GAGGAG	2 cut(s)	447, 633
BseYI	CCCAGC	1 cut(s)	394
Bsh1236I	CGCG	2 cut(s)	204, 315
BshFI	GGCC	6 cut(s)	22, 484, 585, 720, 917, 1045
BsiHKCI	CYCGRG	1 cut(s)	226
BsiSI	CCGG	1 cut(s)	193
BsiWI	CGTACG	1 cut(s)	372
BslFI	GGGAC	2 cut(s)	317, 476
BslI	CCNNNNNNNGG	2 cut(s)	205, 334
BsmAI	GTCTC	2 cut(s)	576, 698
BsmFI	GGGAC	2 cut(s)	317, 476
BsnI	GGCC	6 cut(s)	22, 484, 585, 720, 917, 1045
Bso31I	GGTCTC	1 cut(s)	698
BsoBI	CYCGRG	1 cut(s)	226
Bsp143I	GATC	4 cut(s)	159, 462, 679, 829
Bsp1720I	GCTNAGC	1 cut(s)	845
BspACI	CCGC	4 cut(s)	60, 69, 202, 295
BspANI	GGCC	6 cut(s)	22, 484, 585, 720, 917, 1045
BspCNI	CTCAG	4 cut(s)	472, 710, 837, 850
BspFNI	CGCG	2 cut(s)	204, 315
BspLI	GGNNCC	2 cut(s)	197, 1044
BspMAI	CTGCAG	1 cut(s)	239
BspMI	ACCTGC	2 cut(s)	964, 994
BspTNI	GGTCTC	1 cut(s)	698
BsrDI	GCAATG	1 cut(s)	856
BsrI	ACTGG	2 cut(s)	43, 918
BssECI	CCNNGG	5 cut(s)	268, 580, 586, 688, 712
BssMI	GATC	4 cut(s)	159, 462, 679, 829
BssSI	CACGAG	1 cut(s)	281
BssT1I	CCWWGG	3 cut(s)	580, 586, 688
Bst2BI	CACGAG	1 cut(s)	281
Bst2UI	CCWGG	1 cut(s)	714
Bst4CI	ACNGT	3 cut(s)	137, 546, 790
BstBAI	YACGTR	1 cut(s)	372
BstC8I	GCNNGC	5 cut(s)	11, 235, 297, 329, 486
BstDEI	CTNAG	5 cut(s)	49, 459, 697, 837, 845
BstDSI	CCRYGG	1 cut(s)	268
BstENI	CCTNNNNNAGG	1 cut(s)	203
BstF5I	GGATG	3 cut(s)	98, 153, 1041
BstFNI	CGCG	2 cut(s)	204, 315
BstH2I	RGCGCY	1 cut(s)	234
BstHHI	GCGC	2 cut(s)	233, 315
BstKTI	GATC	4 cut(s)	162, 465, 682, 832
BstMAI	GTCTC	2 cut(s)	576, 698
BstMBI	GATC	4 cut(s)	159, 462, 679, 829
BstMWI	GCNNNNNNNGC	2 cut(s)	19, 292
BstNI	CCWGG	1 cut(s)	714
BstNSI	RCATGY	2 cut(s)	454, 642
BstSCI	CCNGG	1 cut(s)	712
BstSFI	CTRYAG	1 cut(s)	235
BstSNI	TACGTA	1 cut(s)	372
BstUI	CGCG	2 cut(s)	204, 315
BstV2I	GAAGAC	2 cut(s)	600, 906
BstX2I	RGATCY	1 cut(s)	679
BstYI	RGATCY	1 cut(s)	679
BsuRI	GGCC	6 cut(s)	22, 484, 585, 720, 917, 1045
BtgI	CCRYGG	1 cut(s)	268
BtsCI	GGATG	3 cut(s)	98, 153, 1041
BtsIMutI	CAGTG	1 cut(s)	911
BveI	ACCTGC	2 cut(s)	964, 994
Cac8I	GCNNGC	5 cut(s)	11, 235, 297, 329, 486
CaiI	CAGNNNCTG	1 cut(s)	844
CfoI	GCGC	2 cut(s)	233, 315
Cfr13I	GGNCC	1 cut(s)	1043
Csp6I	GTAC	4 cut(s)	352, 369, 373, 382
CviAII	CATG	3 cut(s)	309, 451, 639
CviQI	GTAC	4 cut(s)	352, 369, 373, 382
DdeI	CTNAG	5 cut(s)	49, 459, 697, 837, 845
DpnI	GATC	4 cut(s)	161, 464, 681, 831
DpnII	GATC	4 cut(s)	159, 462, 679, 829
DraI	TTTAAA	1 cut(s)	1074
DraIII	CACNNNGTG	1 cut(s)	274
EaeI	YGGCCR	1 cut(s)	482
Eco105I	TACGTA	1 cut(s)	372
Eco130I	CCWWGG	3 cut(s)	580, 586, 688
Eco31I	GGTCTC	1 cut(s)	698
Eco57I	CTGAAG	1 cut(s)	307
Eco88I	CYCGRG	1 cut(s)	226
EcoNI	CCTNNNNNAGG	1 cut(s)	203
EcoO109I	RGGNCCY	1 cut(s)	1043
EcoRI	GAATTC	1 cut(s)	889
EcoRII	CCWGG	1 cut(s)	712
EcoT14I	CCWWGG	3 cut(s)	580, 586, 688
ErhI	CCWWGG	3 cut(s)	580, 586, 688
FaeI	CATG	3 cut(s)	312, 454, 642
FaiI	YATR	8 cut(s)	65, 310, 452, 640, 876, 1004, 1029, 1083
FalI	AAGNNNNNCTT	2 cut(s)	585, 617
FaqI	GGGAC	2 cut(s)	317, 476
FatI	CATG	3 cut(s)	308, 450, 638
FauI	CCCGC	1 cut(s)	288
Fnu4HI	GCNGC	1 cut(s)	61
FokI	GGATG	3 cut(s)	85, 140, 1048
Fsp4HI	GCNGC	1 cut(s)	61
GlaI	GCGC	2 cut(s)	232, 314
GluI	GCNGC	1 cut(s)	61
GsaI	CCCAGC	1 cut(s)	398
GsuI	CTGGAG	1 cut(s)	356
HaeII	RGCGCY	1 cut(s)	234
HaeIII	GGCC	6 cut(s)	22, 484, 585, 720, 917, 1045
HapII	CCGG	1 cut(s)	193
HhaI	GCGC	2 cut(s)	233, 315
Hin1II	CATG	3 cut(s)	312, 454, 642
Hin6I	GCGC	2 cut(s)	231, 313
HinP1I	GCGC	2 cut(s)	231, 313
HincII	GTYRAC	1 cut(s)	151
HindII	GTYRAC	1 cut(s)	151
HinfI	GANTC	3 cut(s)	39, 553, 1067
HpaII	CCGG	1 cut(s)	193
HphI	GGTGA	5 cut(s)	105, 286, 352, 785, 796
Hpy166II	GTNNAC	4 cut(s)	151, 265, 644, 820
Hpy188I	TCNGA	7 cut(s)	462, 467, 558, 617, 700, 709, 827
Hpy188III	TCNNGA	7 cut(s)	34, 157, 226, 335, 419, 605, 817
Hpy8I	GTNNAC	4 cut(s)	151, 265, 644, 820
Hpy99I	CGWCG	1 cut(s)	827
HpyAV	CCTTC	6 cut(s)	371, 400, 556, 737, 820, 1033
HpyCH4III	ACNGT	3 cut(s)	137, 546, 790
HpyCH4IV	ACGT	2 cut(s)	371, 822
HpyCH4V	TGCA	4 cut(s)	13, 237, 504, 854
HpyF10VI	GCNNNNNNNGC	2 cut(s)	19, 292
HpyF3I	CTNAG	5 cut(s)	49, 459, 697, 837, 845
HpySE526I	ACGT	2 cut(s)	371, 822
Hsp92II	CATG	3 cut(s)	312, 454, 642
HspAI	GCGC	2 cut(s)	231, 313
Kzo9I	GATC	4 cut(s)	159, 462, 679, 829
LmnI	GCTCC	1 cut(s)	195
LweI	GCATC	3 cut(s)	130, 204, 598
MaeII	ACGT	2 cut(s)	371, 822
MaeIII	GTNAC	2 cut(s)	784, 970
MalI	GATC	4 cut(s)	161, 464, 681, 831
MboI	GATC	4 cut(s)	159, 462, 679, 829
MboII	GAAGA	7 cut(s)	313, 434, 530, 542, 605, 635, 911
MflI	RGATCY	1 cut(s)	679
MlsI	TGGCCA	1 cut(s)	484
MluCI	AATT	5 cut(s)	509, 652, 778, 855, 889
MluNI	TGGCCA	1 cut(s)	484
MlyI	GAGTC	1 cut(s)	48
MmeI	TCCRAC	2 cut(s)	381, 805
Mox20I	TGGCCA	1 cut(s)	484
MscI	TGGCCA	1 cut(s)	484
MseI	TTAA	2 cut(s)	540, 1073
MslI	CAYNNNNRTG	1 cut(s)	138
Msp20I	TGGCCA	1 cut(s)	484
MspA1I	CMGCKG	1 cut(s)	488
MspI	CCGG	1 cut(s)	193
MspR9I	CCNGG	1 cut(s)	714
MvaI	CCWGG	1 cut(s)	714
MvnI	CGCG	2 cut(s)	204, 315
MwoI	GCNNNNNNNGC	2 cut(s)	19, 292
NdeII	GATC	4 cut(s)	159, 462, 679, 829
NlaIII	CATG	3 cut(s)	312, 454, 642
NlaIV	GGNNCC	2 cut(s)	197, 1044
NmeAIII	GCCGAG	1 cut(s)	390
NmuCI	GTSAC	1 cut(s)	784
NspI	RCATGY	2 cut(s)	454, 642
PaeR7I	CTCGAG	1 cut(s)	226
PfeI	GAWTC	2 cut(s)	553, 1067
Pfl23II	CGTACG	1 cut(s)	372
PkrI	GCNGC	1 cut(s)	62
PleI	GAGTC	1 cut(s)	47
PpsI	GAGTC	1 cut(s)	47
Ppu21I	YACGTR	1 cut(s)	372
Psp6I	CCWGG	1 cut(s)	712
PspFI	CCCAGC	1 cut(s)	394
PspGI	CCWGG	1 cut(s)	712
PspLI	CGTACG	1 cut(s)	372
PspN4I	GGNNCC	2 cut(s)	197, 1044
PspPI	GGNCC	1 cut(s)	1043
PsrI	GAACNNNNNNTAC	2 cut(s)	963, 995
PstI	CTGCAG	1 cut(s)	239
PstNI	CAGNNNCTG	1 cut(s)	844
PsuI	RGATCY	1 cut(s)	679
PvuII	CAGCTG	1 cut(s)	488
RsaI	GTAC	4 cut(s)	353, 370, 374, 383
RsaNI	GTAC	4 cut(s)	352, 369, 373, 382
RseI	CAYNNNNRTG	1 cut(s)	138
SaqAI	TTAA	2 cut(s)	540, 1073
SatI	GCNGC	1 cut(s)	61
Sau3AI	GATC	4 cut(s)	159, 462, 679, 829
Sau96I	GGNCC	1 cut(s)	1043
SbfI	CCTGCAGG	1 cut(s)	239
ScaI	AGTACT	1 cut(s)	353
SchI	GAGTC	1 cut(s)	48
ScrFI	CCNGG	1 cut(s)	714
SdaI	CCTGCAGG	1 cut(s)	239
SfaNI	GCATC	3 cut(s)	130, 204, 598
SfcI	CTRYAG	1 cut(s)	235
Sfr274I	CTCGAG	1 cut(s)	226
SlaI	CTCGAG	1 cut(s)	226
SmiMI	CAYNNNNRTG	1 cut(s)	138
SmlI	CTYRAG	1 cut(s)	226
SmoI	CTYRAG	1 cut(s)	226
SnaBI	TACGTA	1 cut(s)	372
Sse8387I	CCTGCAGG	1 cut(s)	239
Sse9I	AATT	5 cut(s)	509, 652, 778, 855, 889
SsiI	CCGC	4 cut(s)	60, 69, 202, 295
SspI	AATATT	1 cut(s)	631
StyD4I	CCNGG	1 cut(s)	712
StyI	CCWWGG	3 cut(s)	580, 586, 688
TaaI	ACNGT	3 cut(s)	137, 546, 790
TaiI	ACGT	2 cut(s)	374, 825
TaqI	TCGA	3 cut(s)	227, 606, 982
TasI	AATT	5 cut(s)	509, 652, 778, 855, 889
TatI	WGTACW	1 cut(s)	351
TauI	GCSGC	1 cut(s)	63
TfiI	GAWTC	2 cut(s)	553, 1067
Tru1I	TTAA	2 cut(s)	540, 1073
Tru9I	TTAA	2 cut(s)	540, 1073
TscAI	CASTG	1 cut(s)	918
TseFI	GTSAC	1 cut(s)	784
Tsp45I	GTSAC	1 cut(s)	784
TspDTI	ATGAA	6 cut(s)	305, 434, 575, 756, 802, 1052
TspGWI	ACGGA	1 cut(s)	399
TspRI	CASTG	1 cut(s)	918
XagI	CCTNNNNNAGG	1 cut(s)	203
XapI	RAATTY	1 cut(s)	889
XceI	RCATGY	2 cut(s)	454, 642
XhoI	CTCGAG	1 cut(s)	226
ZrmI	AGTACT	1 cut(s)	353

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.