Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0015650

Physical Location & Seq

Genomic Coordinates Reverse (-)

24806186 .. 24809140

Gene Structure 2955 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1168100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1089 bp

ATGAGTTGCCTGCAGTCTTGGCCTGAGCCTATCGTGAGAGTCCAGTCCTTAGCAGAAAGCGGCATAACCGCAATCCCCGAACTCTACATCAAGCCACCATCCAAACGACCATCGCCGCCAAACACCAACAACCACCGTGATGCTGACCTCAACATCCCGGTCATTGACCTCCAAGACCTTTTGAGCGACAAGCGGAGCCTCCGCGAGTCAACTATGCGTTCGATCTCGAGCGCCTGCAGGGAGTGGGGTTTTTTTCAGGTGGTGAACCACGGGGTGAGCCACGAGCTGATGAAGCGGGCGAGGGACACATGGCGCGAGTTCTTCGGGCAGCCGCTGGAGGTGAAGCAGGGGTACTCCAACAAGCCGAATACGTACGAAGGGTACGGAAGTCGGCTGGGAGTTGAAAAGGGGGCTATTCTGGATTGGAGTGATTATTACTTTCTTCATTACATGCCTCCCCAGCTCAGAAACCACCACAAGTGGCCTGCTCTCCCTTCGTCATGCAGGAGATTGATTGAAGAATACAGTGAAGCAACAGTTCAACTATGTGGGATTCTGATGAGGGTGTTGTCTCTAAACCTTGGTCTTGGGGAAGACCAACTTCTCAACGCTTTCGGAGGAGAAGAGAACATTGGGGCATGCTTACGGGTCAATTTTTACCCAAAATGCCCTCAGCCAGACCTAACCCTTGGTCTCTCAGAACACTCTGACCCAGGTGGCCTGACCCTTCTCTTACCTGATGAAAATGTGGCCGGACTTCAAGTTCGTAAAGGTGAAAACTGGGTGACTGTGACGCCTGTTCCTAATGCCTTCATCGTGAACGTTGGAGATCAAACTCAGGTGCTGAGCAATGCAATTTACAAAAGTGTAGAGCATAGAGTGACTGTGAATTCAGTTAAAGACAGAGTTTCACTGGCCTTGTTTTACAACCCAAGGAGTGATTTGATGATTGAACCTGTCAAAGCTCTTGTCACAAATGATCGACCTGCCCTTTACTTGCCTATGACATTTGATCAGTATCGGCTTTACATTAGGACGAAGGGGCCTTGTGGAAAGGCACAAGTTGAATCTTTAAAATCCTATGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0009966 GO:0010646 GO:0016787 GO:0016810 GO:0023051 GO:0044424 GO:0044444 GO:0044464 GO:0048583 GO:0050789 GO:0050794 GO:0065007 GO:0120091 GO:2000022

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

363

Amino Acids

40.88

Weight (kDa)

6.72

Isoelectric Point (pI)

53.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	52 - 163	1.4e-31	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	213 - 311	2.8e-33	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015650)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g11360
malus_domestica	MD04G1154400.v1.1 MD12G1168100.v1.1
prunus_persica	Prupe.6G278400_v2.0.a1
pyrus_communis	pycom04g13930 pycom12g15960
rosa_chinensis	RchiOBHm_Chr3g0462961
rosa_laevigata	RLG00000024821
rosa_multiflora	Rmu_sc0000766.1_g000023
rosa_roxburghii	Rroxscaffold_6G00417080
rosa_rugosa	Rorug03G0060300
rosa_samantha	Rh3AG118500 Rh3BG123500 Rh3CG125200 Rh3DG124200
rosa_wichuraiana	Rw3G010050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	994
AccII	CGCG	2 cut(s)	204, 315
AciI	CCGC	7 cut(s)	60, 69, 116, 193, 202, 295, 332
AclI	AACGTT	1 cut(s)	822
AcoI	YGGCCR	1 cut(s)	750
AcsI	RAATTY	1 cut(s)	889
AcyI	GRCGYC	1 cut(s)	794
AdeI	CACNNNGTG	1 cut(s)	274
AfaI	GTAC	3 cut(s)	353, 374, 383
AgsI	TTSAA	6 cut(s)	404, 518, 542, 761, 953, 1067
AjnI	CCWGG	1 cut(s)	712
AjuI	GAANNNNNNNTTGG	2 cut(s)	615, 647
AloI	GAACNNNNNNTCC	2 cut(s)	747, 779
AluBI	AGCT	3 cut(s)	286, 463, 965
AluI	AGCT	3 cut(s)	286, 463, 965
Alw26I	GTCTC	2 cut(s)	576, 698
AlwNI	CAGNNNCTG	2 cut(s)	334, 844
Ama87I	CYCGRG	1 cut(s)	226
AoxI	GGCC	6 cut(s)	20, 482, 718, 750, 915, 1043
ApeKI	GCWGC	1 cut(s)	328
ApoI	RAATTY	1 cut(s)	889
ArsI	GACNNNNNNTTYG	4 cut(s)	747, 779, 954, 986
AspLEI	GCGC	2 cut(s)	233, 315
AspS9I	GGNCC	1 cut(s)	1043
AsuC2I	CCSGG	1 cut(s)	158
AsuHPI	GGTGA	5 cut(s)	274, 286, 352, 785, 796
AvaI	CYCGRG	1 cut(s)	226
BaeI	ACNNNNGTAYC	2 cut(s)	373, 406
BarI	GAAGNNNNNNTAC	2 cut(s)	335, 367
BauI	CACGAG	1 cut(s)	281
BbsI	GAAGAC	1 cut(s)	600
BbvCI	CCTCAGC	1 cut(s)	672
BbvI	GCAGC	1 cut(s)	340
BccI	CCATC	2 cut(s)	106, 118
BciT130I	CCWGG	1 cut(s)	714
BclI	TGATCA	1 cut(s)	1012
BcnI	CCSGG	1 cut(s)	158
BcoDI	GTCTC	2 cut(s)	576, 698
BfmI	CTRYAG	2 cut(s)	11, 235
BfoI	RGCGCY	1 cut(s)	234
BfuAI	ACCTGC	1 cut(s)	994
BisI	GCNGC	4 cut(s)	61, 116, 329, 332
BlpI	GCTNAGC	1 cut(s)	845
BlsI	GCNGC	4 cut(s)	62, 117, 330, 333
Bme1390I	CCNGG	2 cut(s)	158, 714
BmeT110I	CYCGRG	1 cut(s)	226
BmgT120I	GGNCC	1 cut(s)	1043
BmiI	GGNNCC	2 cut(s)	197, 1044
BmrFI	CCNGG	2 cut(s)	158, 714
BmrI	ACTGGG	1 cut(s)	790
BmsI	GCATC	1 cut(s)	130
BmuI	ACTGGG	1 cut(s)	790
BpiI	GAAGAC	1 cut(s)	600
BpmI	CTGGAG	1 cut(s)	356
Bpu10I	CCTNAGC	3 cut(s)	24, 49, 672
Bpu1102I	GCTNAGC	1 cut(s)	845
BpuMI	CCSGG	1 cut(s)	158
BsaAI	YACGTR	1 cut(s)	372
BsaBI	GATNNNNATC	1 cut(s)	1017
BsaHI	GRCGYC	1 cut(s)	794
BsaI	GGTCTC	1 cut(s)	698
BsaJI	CCNNGG	5 cut(s)	268, 580, 688, 712, 932
Bse1I	ACTGG	3 cut(s)	43, 785, 918
Bse3DI	GCAATG	1 cut(s)	856
Bse8I	GATNNNNATC	1 cut(s)	1017
BseBI	CCWGG	1 cut(s)	714
BseDI	CCNNGG	5 cut(s)	268, 580, 688, 712, 932
BseGI	GGATG	2 cut(s)	98, 153
BseJI	GATNNNNATC	1 cut(s)	1017
BseMI	GCAATG	1 cut(s)	856
BseMII	CTCAG	6 cut(s)	15, 478, 686, 711, 836, 851
BseNI	ACTGG	3 cut(s)	43, 785, 918
BseRI	GAGGAG	1 cut(s)	633
BseXI	GCAGC	1 cut(s)	340
BseYI	CCCAGC	2 cut(s)	394, 459
Bsh1236I	CGCG	2 cut(s)	204, 315
BshFI	GGCC	6 cut(s)	22, 484, 720, 752, 917, 1045
BsiHKCI	CYCGRG	1 cut(s)	226
BsiSI	CCGG	2 cut(s)	158, 753
BsiWI	CGTACG	1 cut(s)	372
BslFI	GGGAC	1 cut(s)	317
BsmAI	GTCTC	2 cut(s)	576, 698
BsmFI	GGGAC	1 cut(s)	317
BsnI	GGCC	6 cut(s)	22, 484, 720, 752, 917, 1045
Bso31I	GGTCTC	1 cut(s)	698
BsoBI	CYCGRG	1 cut(s)	226
Bsp143I	GATC	4 cut(s)	222, 829, 979, 1012
Bsp1720I	GCTNAGC	1 cut(s)	845
BspACI	CCGC	7 cut(s)	60, 69, 116, 193, 202, 295, 332
BspANI	GGCC	6 cut(s)	22, 484, 720, 752, 917, 1045
BspCNI	CTCAG	6 cut(s)	16, 477, 685, 710, 837, 850
BspFNI	CGCG	2 cut(s)	204, 315
BspLI	GGNNCC	2 cut(s)	197, 1044
BspMAI	CTGCAG	2 cut(s)	15, 239
BspMI	ACCTGC	1 cut(s)	994
BspTNI	GGTCTC	1 cut(s)	698
BsrDI	GCAATG	1 cut(s)	856
BsrI	ACTGG	3 cut(s)	43, 785, 918
BssECI	CCNNGG	5 cut(s)	268, 580, 688, 712, 932
BssMI	GATC	4 cut(s)	222, 829, 979, 1012
BssNI	GRCGYC	1 cut(s)	794
BssSI	CACGAG	1 cut(s)	281
BssT1I	CCWWGG	3 cut(s)	580, 688, 932
Bst2BI	CACGAG	1 cut(s)	281
Bst2UI	CCWGG	1 cut(s)	714
Bst4CI	ACNGT	5 cut(s)	137, 527, 538, 790, 886
Bst6I	CTCTTC	1 cut(s)	618
BstACI	GRCGYC	1 cut(s)	794
BstBAI	YACGTR	1 cut(s)	372
BstC8I	GCNNGC	5 cut(s)	11, 235, 297, 486, 640
BstDEI	CTNAG	7 cut(s)	24, 49, 464, 672, 697, 837, 845
BstDSI	CCRYGG	1 cut(s)	268
BstF5I	GGATG	2 cut(s)	98, 153
BstFNI	CGCG	2 cut(s)	204, 315
BstH2I	RGCGCY	1 cut(s)	234
BstHHI	GCGC	2 cut(s)	233, 315
BstKTI	GATC	4 cut(s)	225, 832, 982, 1015
BstMAI	GTCTC	2 cut(s)	576, 698
BstMBI	GATC	4 cut(s)	222, 829, 979, 1012
BstMWI	GCNNNNNNNGC	3 cut(s)	19, 292, 460
BstNI	CCWGG	1 cut(s)	714
BstNSI	RCATGY	2 cut(s)	454, 642
BstSCI	CCNGG	2 cut(s)	156, 712
BstSFI	CTRYAG	2 cut(s)	11, 235
BstSNI	TACGTA	1 cut(s)	372
BstUI	CGCG	2 cut(s)	204, 315
BstV1I	GCAGC	1 cut(s)	340
BstV2I	GAAGAC	1 cut(s)	600
BsuRI	GGCC	6 cut(s)	22, 484, 720, 752, 917, 1045
BtgI	CCRYGG	1 cut(s)	268
BtgZI	GCGATG	1 cut(s)	96
BtsCI	GGATG	2 cut(s)	98, 153
BtsIMutI	CAGTG	2 cut(s)	532, 911
BveI	ACCTGC	1 cut(s)	994
Cac8I	GCNNGC	5 cut(s)	11, 235, 297, 486, 640
CaiI	CAGNNNCTG	2 cut(s)	334, 844
CfoI	GCGC	2 cut(s)	233, 315
Cfr13I	GGNCC	1 cut(s)	1043
CseI	GACGC	1 cut(s)	802
Csp6I	GTAC	3 cut(s)	352, 373, 382
CviAII	CATG	4 cut(s)	309, 451, 501, 639
CviQI	GTAC	3 cut(s)	352, 373, 382
DdeI	CTNAG	7 cut(s)	24, 49, 464, 672, 697, 837, 845
DpnI	GATC	4 cut(s)	224, 831, 981, 1014
DpnII	GATC	4 cut(s)	222, 829, 979, 1012
DraI	TTTAAA	1 cut(s)	1074
DraIII	CACNNNGTG	1 cut(s)	274
EaeI	YGGCCR	1 cut(s)	750
Eam1104I	CTCTTC	1 cut(s)	618
EarI	CTCTTC	1 cut(s)	618
Eco105I	TACGTA	1 cut(s)	372
Eco130I	CCWWGG	3 cut(s)	580, 688, 932
Eco31I	GGTCTC	1 cut(s)	698
Eco88I	CYCGRG	1 cut(s)	226
EcoO109I	RGGNCCY	1 cut(s)	1043
EcoRI	GAATTC	1 cut(s)	889
EcoRII	CCWGG	1 cut(s)	712
EcoT14I	CCWWGG	3 cut(s)	580, 688, 932
ErhI	CCWWGG	3 cut(s)	580, 688, 932
FaeI	CATG	4 cut(s)	312, 454, 504, 642
FalI	AAGNNNNNCTT	2 cut(s)	585, 617
FaqI	GGGAC	1 cut(s)	317
FatI	CATG	4 cut(s)	308, 450, 500, 638
FauI	CCCGC	1 cut(s)	288
FbaI	TGATCA	1 cut(s)	1012
Fnu4HI	GCNGC	4 cut(s)	61, 116, 329, 332
FokI	GGATG	2 cut(s)	85, 140
Fsp4HI	GCNGC	4 cut(s)	61, 116, 329, 332
GlaI	GCGC	2 cut(s)	232, 314
GluI	GCNGC	4 cut(s)	61, 116, 329, 332
GsaI	CCCAGC	2 cut(s)	398, 463
GsuI	CTGGAG	1 cut(s)	356
HaeII	RGCGCY	1 cut(s)	234
HaeIII	GGCC	6 cut(s)	22, 484, 720, 752, 917, 1045
HapII	CCGG	2 cut(s)	158, 753
HgaI	GACGC	1 cut(s)	802
HhaI	GCGC	2 cut(s)	233, 315
Hin1I	GRCGYC	1 cut(s)	794
Hin1II	CATG	4 cut(s)	312, 454, 504, 642
Hin6I	GCGC	2 cut(s)	231, 313
HinP1I	GCGC	2 cut(s)	231, 313
HincII	GTYRAC	1 cut(s)	210
HindII	GTYRAC	1 cut(s)	210
HinfI	GANTC	4 cut(s)	39, 206, 553, 1067
HpaII	CCGG	2 cut(s)	158, 753
HphI	GGTGA	5 cut(s)	274, 286, 352, 785, 796
Hpy166II	GTNNAC	3 cut(s)	210, 265, 820
Hpy188I	TCNGA	5 cut(s)	467, 558, 617, 700, 709
Hpy188III	TCNNGA	4 cut(s)	34, 226, 419, 817
Hpy8I	GTNNAC	3 cut(s)	210, 265, 820
HpyAV	CCTTC	5 cut(s)	371, 504, 737, 820, 1033
HpyCH4III	ACNGT	5 cut(s)	137, 527, 538, 790, 886
HpyCH4IV	ACGT	2 cut(s)	371, 822
HpyCH4V	TGCA	4 cut(s)	13, 237, 504, 854
HpyF10VI	GCNNNNNNNGC	3 cut(s)	19, 292, 460
HpyF3I	CTNAG	7 cut(s)	24, 49, 464, 672, 697, 837, 845
HpySE526I	ACGT	2 cut(s)	371, 822
Hsp92I	GRCGYC	1 cut(s)	794
Hsp92II	CATG	4 cut(s)	312, 454, 504, 642
HspAI	GCGC	2 cut(s)	231, 313
Ksp22I	TGATCA	1 cut(s)	1012
Kzo9I	GATC	4 cut(s)	222, 829, 979, 1012
LmnI	GCTCC	1 cut(s)	195
Lsp1109I	GCAGC	1 cut(s)	340
LweI	GCATC	1 cut(s)	130
MaeII	ACGT	2 cut(s)	371, 822
MaeIII	GTNAC	4 cut(s)	784, 790, 880, 970
MalI	GATC	4 cut(s)	224, 831, 981, 1014
MboI	GATC	4 cut(s)	222, 829, 979, 1012
MboII	GAAGA	5 cut(s)	313, 434, 530, 605, 635
MluCI	AATT	3 cut(s)	652, 855, 889
MlyI	GAGTC	2 cut(s)	48, 215
MmeI	TCCRAC	2 cut(s)	381, 805
MnlI	CCTC	9 cut(s)	158, 179, 209, 294, 331, 465, 555, 611, 681
MseI	TTAA	2 cut(s)	897, 1073
MslI	CAYNNNNRTG	1 cut(s)	138
MspA1I	CMGCKG	1 cut(s)	334
MspI	CCGG	2 cut(s)	158, 753
MspR9I	CCNGG	2 cut(s)	158, 714
MvaI	CCWGG	1 cut(s)	714
MvnI	CGCG	2 cut(s)	204, 315
MwoI	GCNNNNNNNGC	3 cut(s)	19, 292, 460
NciI	CCSGG	1 cut(s)	158
NdeII	GATC	4 cut(s)	222, 829, 979, 1012
NlaIII	CATG	4 cut(s)	312, 454, 504, 642
NlaIV	GGNNCC	2 cut(s)	197, 1044
NmuCI	GTSAC	4 cut(s)	784, 790, 880, 970
NspI	RCATGY	2 cut(s)	454, 642
PaeI	GCATGC	1 cut(s)	642
PaeR7I	CTCGAG	1 cut(s)	226
PfeI	GAWTC	2 cut(s)	553, 1067
Pfl23II	CGTACG	1 cut(s)	372
PkrI	GCNGC	4 cut(s)	62, 117, 330, 333
PleI	GAGTC	2 cut(s)	47, 214
PpsI	GAGTC	2 cut(s)	47, 214
Ppu21I	YACGTR	1 cut(s)	372
Psp1406I	AACGTT	1 cut(s)	822
Psp6I	CCWGG	1 cut(s)	712
PspFI	CCCAGC	2 cut(s)	394, 459
PspGI	CCWGG	1 cut(s)	712
PspLI	CGTACG	1 cut(s)	372
PspN4I	GGNNCC	2 cut(s)	197, 1044
PspPI	GGNCC	1 cut(s)	1043
PstI	CTGCAG	2 cut(s)	15, 239
PstNI	CAGNNNCTG	2 cut(s)	334, 844
RsaI	GTAC	3 cut(s)	353, 374, 383
RsaNI	GTAC	3 cut(s)	352, 373, 382
RseI	CAYNNNNRTG	1 cut(s)	138
SaqAI	TTAA	2 cut(s)	897, 1073
SatI	GCNGC	4 cut(s)	61, 116, 329, 332
Sau3AI	GATC	4 cut(s)	222, 829, 979, 1012
Sau96I	GGNCC	1 cut(s)	1043
SbfI	CCTGCAGG	1 cut(s)	239
SchI	GAGTC	2 cut(s)	48, 215
ScrFI	CCNGG	2 cut(s)	158, 714
SdaI	CCTGCAGG	1 cut(s)	239
SfaNI	GCATC	1 cut(s)	130
SfcI	CTRYAG	2 cut(s)	11, 235
Sfr274I	CTCGAG	1 cut(s)	226
SlaI	CTCGAG	1 cut(s)	226
SmiMI	CAYNNNNRTG	1 cut(s)	138
SmlI	CTYRAG	1 cut(s)	226
SmoI	CTYRAG	1 cut(s)	226
SnaBI	TACGTA	1 cut(s)	372
SphI	GCATGC	1 cut(s)	642
Sse8387I	CCTGCAGG	1 cut(s)	239
Sse9I	AATT	3 cut(s)	652, 855, 889
SsiI	CCGC	7 cut(s)	60, 69, 116, 193, 202, 295, 332
StyD4I	CCNGG	2 cut(s)	156, 712
StyI	CCWWGG	3 cut(s)	580, 688, 932
TaaI	ACNGT	5 cut(s)	137, 527, 538, 790, 886
TaiI	ACGT	2 cut(s)	374, 825
TaqI	TCGA	3 cut(s)	221, 227, 982
TasI	AATT	3 cut(s)	652, 855, 889
TauI	GCSGC	3 cut(s)	63, 118, 334
TfiI	GAWTC	2 cut(s)	553, 1067
Tru1I	TTAA	2 cut(s)	897, 1073
Tru9I	TTAA	2 cut(s)	897, 1073
TscAI	CASTG	2 cut(s)	532, 918
TseFI	GTSAC	4 cut(s)	784, 790, 880, 970
TseI	GCWGC	1 cut(s)	328
Tsp45I	GTSAC	4 cut(s)	784, 790, 880, 970
TspDTI	ATGAA	4 cut(s)	305, 434, 756, 802
TspGWI	ACGGA	1 cut(s)	399
TspRI	CASTG	2 cut(s)	532, 918
XapI	RAATTY	1 cut(s)	889
XceI	RCATGY	2 cut(s)	454, 642
XhoI	CTCGAG	1 cut(s)	226

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.