Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0015650

Physical Location & Seq

Genomic Coordinates Reverse (-)

39387790 .. 39389250

Gene Structure 1461 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000024821

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1086 bp

ATGATGAACTGTTTGCAGAGCTGGCCGGAGCCGATTGTTCGGGTCCAGTCCCTCGCCGAAAGCGGGATAAAAACAATCCCGGAGCTGTACATCAAGCCCCCTTGCAAACGACCCTCGAATAATCTCGTCCTCGACGATGCCACCGTCAACATCCCGGTCATCGACCTGCAGGCCCTGGCCGGAGACGACCAGAGCCTGCGAGAGACCACGCTGCAGCTGGTCTCGAGAGCCTGTAGGGAGTGGGGGTTCTTCCAGGTGGTGAACCACGGTGTCAGCCACGAGCTGATGAAGCGGGCGAGGGAGACATGGCGGGAGTTCTTCGAGCAGCCGCTGGAGGTGAAGCAGGAGTACTCGAACAACCCGAGCACCTATGAAGGGTACGGAAGCCGGCTCGGAGTGGAGAAGGGGGCTATTCTGGATTGGAGTGACTATTACTTTCTTCATTACATGCCTCCGGAGTTGAGGAAGCCCACCAAGTGGCCTGCTCTCCCATCTTCATGCAGGAATTTGATTGAAGAATACGGTGAGGAAGCTGCTAGACTATGTGGAAGACTGATGAAGATGCTATCACTAAACCTTGGGCTAGGGGAAGACTACCTTCTCAATGCTTTTGGAGGAGAAGAGAACGTTGGGGCATGCTTAAGGGTCAATTTCTACCCAAAATGCCCCCAACCTGACCTAACCCTCGGAATCTCGGCACACTCGGACCCCGGTGGGATGACCCTTCTCCTACCCGACGAGGATGTGGCGGGACTTCAAGTTCTTAAAGATGACAGTTGGGTGACTGTAAAGCCCGTCCCGAACGCCTTCATTGTCAACATGGGAGATCAAATGCAAGTTCTGAGCAACGCCATATACAAGAGCGTAGAGCATAGAGTGATTGTGAATTCAGTCAAAGACCGAGTTTCTCTGGCCATGTTTTATAACCCCAAGAGCGATTTGCTAATTGAACCGGCCAAGGCTCTCGTCACGAATGACCGGCCGGCTCTCTACCCGGCTATGACGTTCGATGAGTACCGCCTTTACATTAGGACCAAGGGCCTTTGGGGCAAGGCACAAGTTGAATCCTTGAAAACCTATGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0009966 GO:0010646 GO:0016787 GO:0016810 GO:0023051 GO:0044424 GO:0044444 GO:0044464 GO:0048583 GO:0050789 GO:0050794 GO:0065007 GO:0120091 GO:2000022

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

362

Amino Acids

40.84

Weight (kDa)

5.36

Isoelectric Point (pI)

47.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	51 - 162	6.2e-33	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	212 - 310	6.3e-34	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015650)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g11360
malus_domestica	MD04G1154400.v1.1 MD12G1168100.v1.1
prunus_persica	Prupe.6G278400_v2.0.a1
pyrus_communis	pycom04g13930 pycom12g15960
rosa_chinensis	RchiOBHm_Chr3g0462961
rosa_laevigata	RLG00000024821
rosa_multiflora	Rmu_sc0000766.1_g000023
rosa_roxburghii	Rroxscaffold_6G00417080
rosa_rugosa	Rorug03G0060300
rosa_samantha	Rh3AG118500 Rh3BG123500 Rh3CG125200 Rh3DG124200
rosa_wichuraiana	Rw3G010050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	924
Acc36I	ACCTGC	1 cut(s)	174
AccB7I	CCANNNNNTGG	1 cut(s)	477
AccIII	TCCGGA	1 cut(s)	454
AciI	CCGC	6 cut(s)	63, 292, 310, 329, 749, 1018
AclI	AACGTT	1 cut(s)	627
AcoI	YGGCCR	5 cut(s)	23, 177, 912, 954, 980
AcsI	RAATTY	2 cut(s)	505, 886
AdeI	CACNNNGTG	1 cut(s)	477
AfaI	GTAC	4 cut(s)	89, 350, 380, 1016
AfiI	CCNNNNNNNGG	3 cut(s)	63, 375, 477
AflII	CTTAAG	1 cut(s)	640
AgsI	TTSAA	5 cut(s)	515, 758, 950, 1064, 1072
AjnI	CCWGG	2 cut(s)	174, 252
AjuI	GAANNNNNNNTTGG	2 cut(s)	612, 644
AloI	GAACNNNNNNTCC	4 cut(s)	230, 262, 744, 776
AluBI	AGCT	5 cut(s)	21, 85, 217, 283, 533
AluI	AGCT	5 cut(s)	21, 85, 217, 283, 533
Alw21I	GWGCWC	1 cut(s)	368
Alw26I	GTCTC	4 cut(s)	177, 197, 226, 296
AlwNI	CAGNNNCTG	3 cut(s)	175, 196, 331
Ama87I	CYCGRG	2 cut(s)	223, 361
Aor13HI	TCCGGA	1 cut(s)	454
AoxI	GGCC	8 cut(s)	23, 171, 177, 479, 912, 954, 980, 1039
ApeKI	GCWGC	4 cut(s)	211, 214, 325, 533
ApoI	RAATTY	2 cut(s)	505, 886
Asp700I	GAANNNNTTC	1 cut(s)	806
AspS9I	GGNCC	5 cut(s)	43, 172, 706, 1032, 1039
AsuC2I	CCSGG	4 cut(s)	80, 155, 711, 995
AsuHPI	GGTGA	4 cut(s)	271, 349, 536, 793
AvaI	CYCGRG	2 cut(s)	223, 361
AvaII	GGWCC	3 cut(s)	43, 706, 1032
BalI	TGGCCA	1 cut(s)	914
BarI	GAAGNNNNNNTAC	2 cut(s)	332, 364
BauI	CACGAG	1 cut(s)	278
BbsI	GAAGAC	2 cut(s)	556, 597
Bbv12I	GWGCWC	1 cut(s)	368
BbvI	GCAGC	4 cut(s)	198, 226, 337, 520
BccI	CCATC	1 cut(s)	499
BciT130I	CCWGG	2 cut(s)	176, 254
BcnI	CCSGG	4 cut(s)	80, 155, 711, 995
BcoDI	GTCTC	4 cut(s)	177, 197, 226, 296
BfaI	CTAG	2 cut(s)	537, 584
BfmI	CTRYAG	3 cut(s)	167, 212, 232
BfrI	CTTAAG	1 cut(s)	640
BfuAI	ACCTGC	1 cut(s)	174
BglI	GCCNNNNNGGC	1 cut(s)	1047
BisI	GCNGC	5 cut(s)	212, 215, 326, 329, 534
BlsI	GCNGC	5 cut(s)	213, 216, 327, 330, 535
BmcAI	AGTACT	1 cut(s)	350
Bme1390I	CCNGG	6 cut(s)	80, 155, 176, 254, 711, 995
Bme18I	GGWCC	3 cut(s)	43, 706, 1032
BmeT110I	CYCGRG	2 cut(s)	223, 361
BmgT120I	GGNCC	5 cut(s)	43, 172, 706, 1032, 1039
BmiI	GGNNCC	3 cut(s)	30, 44, 708
BmrFI	CCNGG	6 cut(s)	80, 155, 176, 254, 711, 995
BmsI	GCATC	2 cut(s)	127, 552
BpiI	GAAGAC	2 cut(s)	556, 597
BpmI	CTGGAG	1 cut(s)	353
BpuMI	CCSGG	4 cut(s)	80, 155, 711, 995
BsaI	GGTCTC	2 cut(s)	197, 226
BsaJI	CCNNGG	7 cut(s)	174, 265, 577, 685, 709, 957, 1035
BsaWI	WCCGGW	1 cut(s)	454
BsaXI	ACNNNNNCTCC	2 cut(s)	230, 260
Bsc4I	CCNNNNNNNGG	3 cut(s)	63, 375, 477
Bse118I	RCCGGY	4 cut(s)	387, 952, 978, 982
Bse1I	ACTGG	1 cut(s)	46
BseAI	TCCGGA	1 cut(s)	454
BseBI	CCWGG	2 cut(s)	176, 254
BseDI	CCNNGG	7 cut(s)	174, 265, 577, 685, 709, 957, 1035
BseGI	GGATG	3 cut(s)	150, 723, 748
BseLI	CCNNNNNNNGG	3 cut(s)	63, 375, 477
BseMII	CTCAG	1 cut(s)	833
BseNI	ACTGG	1 cut(s)	46
BseRI	GAGGAG	1 cut(s)	630
BseX3I	CGGCCG	1 cut(s)	980
BseXI	GCAGC	4 cut(s)	198, 226, 337, 520
Bsh1285I	CGRYCG	1 cut(s)	983
BshFI	GGCC	8 cut(s)	25, 173, 179, 481, 914, 956, 982, 1041
BsiEI	CGRYCG	1 cut(s)	983
BsiHKAI	GWGCWC	1 cut(s)	368
BsiHKCI	CYCGRG	2 cut(s)	223, 361
BslFI	GGGAC	3 cut(s)	34, 765, 782
BslI	CCNNNNNNNGG	3 cut(s)	63, 375, 477
BsmAI	GTCTC	4 cut(s)	177, 197, 226, 296
BsmBI	CGTCTC	1 cut(s)	177
BsmFI	GGGAC	3 cut(s)	34, 765, 782
BsnI	GGCC	8 cut(s)	25, 173, 179, 481, 914, 956, 982, 1041
Bso31I	GGTCTC	2 cut(s)	197, 226
BsoBI	CYCGRG	2 cut(s)	223, 361
Bsp1286I	GDGCHC	1 cut(s)	368
Bsp13I	TCCGGA	1 cut(s)	454
Bsp1407I	TGTACA	1 cut(s)	87
Bsp143I	GATC	1 cut(s)	826
BspACI	CCGC	6 cut(s)	63, 292, 310, 329, 749, 1018
BspANI	GGCC	8 cut(s)	25, 173, 179, 481, 914, 956, 982, 1041
BspCNI	CTCAG	1 cut(s)	834
BspEI	TCCGGA	1 cut(s)	454
BspLI	GGNNCC	3 cut(s)	30, 44, 708
BspMAI	CTGCAG	2 cut(s)	171, 216
BspMI	ACCTGC	1 cut(s)	174
BspTI	CTTAAG	1 cut(s)	640
BspTNI	GGTCTC	2 cut(s)	197, 226
BsrFI	RCCGGY	4 cut(s)	387, 952, 978, 982
BsrGI	TGTACA	1 cut(s)	87
BsrI	ACTGG	1 cut(s)	46
BssAI	RCCGGY	4 cut(s)	387, 952, 978, 982
BssECI	CCNNGG	7 cut(s)	174, 265, 577, 685, 709, 957, 1035
BssMI	GATC	1 cut(s)	826
BssSI	CACGAG	1 cut(s)	278
BssT1I	CCWWGG	3 cut(s)	577, 957, 1035
Bst2BI	CACGAG	1 cut(s)	278
Bst2UI	CCWGG	2 cut(s)	176, 254
Bst4CI	ACNGT	6 cut(s)	11, 145, 269, 524, 776, 787
Bst6I	CTCTTC	1 cut(s)	615
BstAFI	CTTAAG	1 cut(s)	640
BstAUI	TGTACA	1 cut(s)	87
BstC8I	GCNNGC	8 cut(s)	23, 171, 197, 294, 389, 483, 637, 984
BstDEI	CTNAG	1 cut(s)	842
BstDSI	CCRYGG	1 cut(s)	265
BstF5I	GGATG	3 cut(s)	150, 723, 748
BstKTI	GATC	1 cut(s)	829
BstMAI	GTCTC	4 cut(s)	177, 197, 226, 296
BstMBI	GATC	1 cut(s)	826
BstMCI	CGRYCG	1 cut(s)	983
BstMWI	GCNNNNNNNGC	3 cut(s)	22, 289, 1047
BstNI	CCWGG	2 cut(s)	176, 254
BstNSI	RCATGY	2 cut(s)	451, 639
BstSCI	CCNGG	6 cut(s)	78, 153, 174, 252, 709, 993
BstSFI	CTRYAG	3 cut(s)	167, 212, 232
BstV1I	GCAGC	4 cut(s)	198, 226, 337, 520
BstV2I	GAAGAC	2 cut(s)	556, 597
BstZI	CGGCCG	1 cut(s)	980
BsuRI	GGCC	8 cut(s)	25, 173, 179, 481, 914, 956, 982, 1041
BtgI	CCRYGG	1 cut(s)	265
BtsCI	GGATG	3 cut(s)	150, 723, 748
BveI	ACCTGC	1 cut(s)	174
Cac8I	GCNNGC	8 cut(s)	23, 171, 197, 294, 389, 483, 637, 984
CaiI	CAGNNNCTG	3 cut(s)	175, 196, 331
Cfr10I	RCCGGY	4 cut(s)	387, 952, 978, 982
Cfr13I	GGNCC	5 cut(s)	43, 172, 706, 1032, 1039
Csp6I	GTAC	4 cut(s)	88, 349, 379, 1015
CviAII	CATG	6 cut(s)	306, 448, 498, 636, 820, 916
CviQI	GTAC	4 cut(s)	88, 349, 379, 1015
DdeI	CTNAG	1 cut(s)	842
DpnI	GATC	1 cut(s)	828
DpnII	GATC	1 cut(s)	826
DraIII	CACNNNGTG	1 cut(s)	477
EaeI	YGGCCR	5 cut(s)	23, 177, 912, 954, 980
EagI	CGGCCG	1 cut(s)	980
Eam1104I	CTCTTC	1 cut(s)	615
EarI	CTCTTC	1 cut(s)	615
EclXI	CGGCCG	1 cut(s)	980
Eco130I	CCWWGG	3 cut(s)	577, 957, 1035
Eco31I	GGTCTC	2 cut(s)	197, 226
Eco47I	GGWCC	3 cut(s)	43, 706, 1032
Eco52I	CGGCCG	1 cut(s)	980
Eco88I	CYCGRG	2 cut(s)	223, 361
EcoO109I	RGGNCCY	2 cut(s)	172, 1039
EcoRI	GAATTC	1 cut(s)	886
EcoRII	CCWGG	2 cut(s)	174, 252
EcoT14I	CCWWGG	3 cut(s)	577, 957, 1035
ErhI	CCWWGG	3 cut(s)	577, 957, 1035
Esp3I	CGTCTC	1 cut(s)	177
FaeI	CATG	6 cut(s)	309, 451, 501, 639, 823, 919
FalI	AAGNNNNNCTT	2 cut(s)	582, 614
FaqI	GGGAC	3 cut(s)	34, 765, 782
FatI	CATG	6 cut(s)	305, 447, 497, 635, 819, 915
FauI	CCCGC	4 cut(s)	56, 285, 303, 742
Fnu4HI	GCNGC	5 cut(s)	212, 215, 326, 329, 534
FokI	GGATG	3 cut(s)	137, 730, 755
Fsp4HI	GCNGC	5 cut(s)	212, 215, 326, 329, 534
FspBI	CTAG	2 cut(s)	537, 584
GluI	GCNGC	5 cut(s)	212, 215, 326, 329, 534
GsuI	CTGGAG	1 cut(s)	353
HaeIII	GGCC	8 cut(s)	25, 173, 179, 481, 914, 956, 982, 1041
Hin1II	CATG	6 cut(s)	309, 451, 501, 639, 823, 919
HincII	GTYRAC	2 cut(s)	148, 817
HindII	GTYRAC	2 cut(s)	148, 817
HinfI	GANTC	2 cut(s)	690, 1064
HphI	GGTGA	4 cut(s)	271, 349, 536, 793
Hpy166II	GTNNAC	3 cut(s)	148, 262, 817
Hpy188I	TCNGA	4 cut(s)	395, 689, 706, 843
Hpy188III	TCNNGA	6 cut(s)	223, 225, 416, 455, 799, 970
Hpy8I	GTNNAC	3 cut(s)	148, 262, 817
Hpy99I	CGWCG	2 cut(s)	137, 740
HpyAV	CCTTC	5 cut(s)	368, 397, 608, 734, 817
HpyCH4III	ACNGT	6 cut(s)	11, 145, 269, 524, 776, 787
HpyCH4IV	ACGT	2 cut(s)	627, 1004
HpyCH4V	TGCA	6 cut(s)	16, 105, 169, 214, 501, 835
HpyF10VI	GCNNNNNNNGC	3 cut(s)	22, 289, 1047
HpyF3I	CTNAG	1 cut(s)	842
HpySE526I	ACGT	2 cut(s)	627, 1004
Hsp92II	CATG	6 cut(s)	309, 451, 501, 639, 823, 919
Kpn2I	TCCGGA	1 cut(s)	454
KroI	GCCGGC	2 cut(s)	387, 982
KroNI	GCCGGC	2 cut(s)	389, 984
Kzo9I	GATC	1 cut(s)	826
LmnI	GCTCC	2 cut(s)	28, 82
Lsp1109I	GCAGC	4 cut(s)	198, 226, 337, 520
LweI	GCATC	2 cut(s)	127, 552
MaeI	CTAG	2 cut(s)	537, 584
MaeII	ACGT	2 cut(s)	627, 1004
MaeIII	GTNAC	3 cut(s)	425, 781, 967
MalI	GATC	1 cut(s)	828
MboI	GATC	1 cut(s)	826
MboII	GAAGA	9 cut(s)	241, 310, 431, 486, 527, 561, 571, 602, 632
MhlI	GDGCHC	1 cut(s)	368
MlsI	TGGCCA	1 cut(s)	914
MluCI	AATT	4 cut(s)	505, 649, 886, 945
MluNI	TGGCCA	1 cut(s)	914
Mox20I	TGGCCA	1 cut(s)	914
MroI	TCCGGA	1 cut(s)	454
MroNI	GCCGGC	2 cut(s)	387, 982
MroXI	GAANNNNTTC	1 cut(s)	806
MscI	TGGCCA	1 cut(s)	914
MseI	TTAA	2 cut(s)	641, 765
MslI	CAYNNNNRTG	1 cut(s)	496
Msp20I	TGGCCA	1 cut(s)	914
MspA1I	CMGCKG	2 cut(s)	217, 331
MspCI	CTTAAG	1 cut(s)	640
MspR9I	CCNGG	6 cut(s)	80, 155, 176, 254, 711, 995
MvaI	CCWGG	2 cut(s)	176, 254
MwoI	GCNNNNNNNGC	3 cut(s)	22, 289, 1047
NaeI	GCCGGC	2 cut(s)	389, 984
NciI	CCSGG	4 cut(s)	80, 155, 711, 995
NdeII	GATC	1 cut(s)	826
NgoMIV	GCCGGC	2 cut(s)	387, 982
NlaIII	CATG	6 cut(s)	309, 451, 501, 639, 823, 919
NlaIV	GGNNCC	3 cut(s)	30, 44, 708
NmeAIII	GCCGAG	1 cut(s)	674
NmuCI	GTSAC	3 cut(s)	425, 781, 967
NspI	RCATGY	2 cut(s)	451, 639
PaeI	GCATGC	1 cut(s)	639
PaeR7I	CTCGAG	1 cut(s)	223
PcsI	WCGNNNNNNNCGW	3 cut(s)	132, 141, 359
PdiI	GCCGGC	2 cut(s)	389, 984
PdmI	GAANNNNTTC	1 cut(s)	806
PfeI	GAWTC	2 cut(s)	690, 1064
PflMI	CCANNNNNTGG	1 cut(s)	477
PfoI	TCCNGGA	1 cut(s)	78
PkrI	GCNGC	5 cut(s)	213, 216, 327, 330, 535
PsiI	TTATAA	1 cut(s)	924
Psp1406I	AACGTT	1 cut(s)	627
Psp6I	CCWGG	2 cut(s)	174, 252
PspGI	CCWGG	2 cut(s)	174, 252
PspN4I	GGNNCC	3 cut(s)	30, 44, 708
PspPI	GGNCC	5 cut(s)	43, 172, 706, 1032, 1039
PstI	CTGCAG	2 cut(s)	171, 216
PstNI	CAGNNNCTG	3 cut(s)	175, 196, 331
PvuII	CAGCTG	1 cut(s)	217
RsaI	GTAC	4 cut(s)	89, 350, 380, 1016
RsaNI	GTAC	4 cut(s)	88, 349, 379, 1015
RseI	CAYNNNNRTG	1 cut(s)	496
SaqAI	TTAA	2 cut(s)	641, 765
SatI	GCNGC	5 cut(s)	212, 215, 326, 329, 534
Sau3AI	GATC	1 cut(s)	826
Sau96I	GGNCC	5 cut(s)	43, 172, 706, 1032, 1039
SbfI	CCTGCAGG	1 cut(s)	171
ScaI	AGTACT	1 cut(s)	350
ScrFI	CCNGG	6 cut(s)	80, 155, 176, 254, 711, 995
SdaI	CCTGCAGG	1 cut(s)	171
SduI	GDGCHC	1 cut(s)	368
SfaNI	GCATC	2 cut(s)	127, 552
SfcI	CTRYAG	3 cut(s)	167, 212, 232
Sfr274I	CTCGAG	1 cut(s)	223
SinI	GGWCC	3 cut(s)	43, 706, 1032
SlaI	CTCGAG	1 cut(s)	223
SmiMI	CAYNNNNRTG	1 cut(s)	496
SmlI	CTYRAG	2 cut(s)	223, 640
SmoI	CTYRAG	2 cut(s)	223, 640
SphI	GCATGC	1 cut(s)	639
Sse8387I	CCTGCAGG	1 cut(s)	171
Sse9I	AATT	4 cut(s)	505, 649, 886, 945
SsiI	CCGC	6 cut(s)	63, 292, 310, 329, 749, 1018
SspMI	CTAG	2 cut(s)	537, 584
StyD4I	CCNGG	6 cut(s)	78, 153, 174, 252, 709, 993
StyI	CCWWGG	3 cut(s)	577, 957, 1035
TaaI	ACNGT	6 cut(s)	11, 145, 269, 524, 776, 787
TaiI	ACGT	2 cut(s)	630, 1007
TaqI	TCGA	7 cut(s)	116, 132, 162, 224, 321, 353, 1008
TaqII	GACCGA	1 cut(s)	915
TasI	AATT	4 cut(s)	505, 649, 886, 945
TatI	WGTACW	2 cut(s)	87, 348
TauI	GCSGC	1 cut(s)	331
TfiI	GAWTC	2 cut(s)	690, 1064
Tru1I	TTAA	2 cut(s)	641, 765
Tru9I	TTAA	2 cut(s)	641, 765
TseFI	GTSAC	3 cut(s)	425, 781, 967
TseI	GCWGC	4 cut(s)	211, 214, 325, 533
Tsp45I	GTSAC	3 cut(s)	425, 781, 967
TspDTI	ATGAA	7 cut(s)	20, 302, 387, 431, 486, 572, 799
TspGWI	ACGGA	1 cut(s)	396
Van91I	CCANNNNNTGG	1 cut(s)	477
Vha464I	CTTAAG	1 cut(s)	640
VpaK11BI	GGWCC	3 cut(s)	43, 706, 1032
XapI	RAATTY	2 cut(s)	505, 886
XceI	RCATGY	2 cut(s)	451, 639
XcmI	CCANNNNNNNNNTGG	1 cut(s)	214
XhoI	CTCGAG	1 cut(s)	223
XmnI	GAANNNNTTC	1 cut(s)	806
XspI	CTAG	2 cut(s)	537, 584
ZrmI	AGTACT	1 cut(s)	350

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.