FvH4_6g13620

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0017688
Physical Location & Seq
Reverse (-)
8283075 .. 8284357
1283 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g13620.t1

Sequence Viewer

Length: 465 bp
ATGTTCCTAATATCAAAGAGAGAATTTGGAGAGATAGAGATGGGAGATCGAGCTGGTGTGCTGGAGGTGAAGCTGATTGAAGCCAGAGGCCTCAAAGACATGGATTTTGCTGTGAAGATGAACACATACGTTGTGATCGAATATGGAAATCAGAAGCAAACAAGCTGCGTGGCTAAAGGAAAAGGAACGAAACCAGTGTGGAATGAAACATTTAAATTGAAGGCAGAATATCCGGGTGGGGAAGATCACAAGTACATGCTTCATTTTCGTATCATGAACACGCATAAGATCTTGGAGCACGATGTATTTGTTGGACAATCTACAATTTATGTGAAGGATTTGATAGCTTCTGGCATGGACACAGGGAAGGCTGAACTTCGCAGCGCCAAGTATAGAGTTGTTATTCAAGACAAAACTTATGCGGGAGAGATCTGTGTGGCTTTATCTTTCTCCATAAACCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

155

Amino Acids

17.45

Weight (kDa)

8.44

Isoelectric Point (pI)

31.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 19 - 118 3.1e-17 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017688)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g13620
malus_domestica MD12G1142700.v1.1
prunus_persica Prupe.6G256400_v2.0.a1
rosa_laevigata RLG00000024571
rosa_multiflora Rmu_sc0003332.1_g000022
rosa_roxburghii Rroxscaffold_6G00414440
rosa_rugosa Rorug03G0082100 Rorug03G0082200
rosa_samantha Rh3AG128800 Rh3BG150100 Rh3DG150800
rosa_wichuraiana Rw3G012180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 422
AcsI RAATTY 1 cut(s) 23
AfaI GTAC 1 cut(s) 254
AgsI TTSAA 3 cut(s) 80, 220, 407
AluBI AGCT 4 cut(s) 53, 73, 165, 347
AluI AGCT 4 cut(s) 53, 73, 165, 347
Alw21I GWGCWC 1 cut(s) 300
AoxI GGCC 1 cut(s) 88
ApeKI GCWGC 2 cut(s) 165, 381
ApoI RAATTY 1 cut(s) 23
ArsI GACNNNNNNTTYG 2 cut(s) 89, 121
AspLEI GCGC 1 cut(s) 386
AsuC2I CCSGG 1 cut(s) 234
AsuHPI GGTGA 1 cut(s) 79
Bbv12I GWGCWC 1 cut(s) 300
BbvI GCAGC 2 cut(s) 152, 393
BccI CCATC 1 cut(s) 34
BcnI CCSGG 1 cut(s) 234
BfoI RGCGCY 1 cut(s) 387
BglII AGATCT 2 cut(s) 288, 429
BisI GCNGC 2 cut(s) 166, 382
BlsI GCNGC 2 cut(s) 167, 383
Bme1390I CCNGG 1 cut(s) 234
BmrFI CCNGG 1 cut(s) 234
BpmI CTGGAG 1 cut(s) 83
BpuMI CCSGG 1 cut(s) 234
Bse1I ACTGG 2 cut(s) 194, 460
BseNI ACTGG 2 cut(s) 194, 460
BseXI GCAGC 2 cut(s) 152, 393
BshFI GGCC 1 cut(s) 90
BsiHKAI GWGCWC 1 cut(s) 300
BsiSI CCGG 1 cut(s) 233
BsnI GGCC 1 cut(s) 90
Bsp1286I GDGCHC 1 cut(s) 300
Bsp143I GATC 5 cut(s) 46, 135, 244, 288, 429
BspACI CCGC 1 cut(s) 422
BspANI GGCC 1 cut(s) 90
BspHI TCATGA 1 cut(s) 273
BsrI ACTGG 2 cut(s) 194, 460
BssMI GATC 5 cut(s) 46, 135, 244, 288, 429
BstH2I RGCGCY 1 cut(s) 387
BstHHI GCGC 1 cut(s) 386
BstKTI GATC 5 cut(s) 49, 138, 247, 291, 432
BstMBI GATC 5 cut(s) 46, 135, 244, 288, 429
BstNSI RCATGY 1 cut(s) 259
BstSCI CCNGG 1 cut(s) 232
BstV1I GCAGC 2 cut(s) 152, 393
BstX2I RGATCY 2 cut(s) 288, 429
BstYI RGATCY 2 cut(s) 288, 429
BsuRI GGCC 1 cut(s) 90
BtsIMutI CAGTG 1 cut(s) 201
CciI TCATGA 1 cut(s) 273
CfoI GCGC 1 cut(s) 386
Csp6I GTAC 1 cut(s) 253
CspCI CAANNNNNGTGG 2 cut(s) 150, 185
CviAII CATG 4 cut(s) 100, 256, 274, 355
CviJI RGCY 9 cut(s) 53, 73, 83, 90, 165, 173, 347, 371, 440
CviKI_1 RGCY 9 cut(s) 53, 73, 83, 90, 165, 173, 347, 371, 440
CviQI GTAC 1 cut(s) 253
DpnI GATC 5 cut(s) 48, 137, 246, 290, 431
DpnII GATC 5 cut(s) 46, 135, 244, 288, 429
DraI TTTAAA 1 cut(s) 214
Eco147I AGGCCT 1 cut(s) 90
FaeI CATG 4 cut(s) 103, 259, 277, 358
FatI CATG 4 cut(s) 99, 255, 273, 354
FauI CCCGC 1 cut(s) 415
Fnu4HI GCNGC 2 cut(s) 166, 382
Fsp4HI GCNGC 2 cut(s) 166, 382
GlaI GCGC 1 cut(s) 385
GluI GCNGC 2 cut(s) 166, 382
GsuI CTGGAG 1 cut(s) 83
HaeII RGCGCY 1 cut(s) 387
HaeIII GGCC 1 cut(s) 90
HapII CCGG 1 cut(s) 233
HhaI GCGC 1 cut(s) 386
Hin1II CATG 4 cut(s) 103, 259, 277, 358
Hin6I GCGC 1 cut(s) 384
HinP1I GCGC 1 cut(s) 384
HpaII CCGG 1 cut(s) 233
HphI GGTGA 1 cut(s) 79
Hpy188I TCNGA 1 cut(s) 153
Hpy188III TCNNGA 2 cut(s) 274, 407
HpyAV CCTTC 3 cut(s) 214, 328, 361
HpyCH4IV ACGT 1 cut(s) 129
HpySE526I ACGT 1 cut(s) 129
Hsp92II CATG 4 cut(s) 103, 259, 277, 358
HspAI GCGC 1 cut(s) 384
Kzo9I GATC 5 cut(s) 46, 135, 244, 288, 429
LmnI GCTCC 1 cut(s) 295
LpnPI CCDG 7 cut(s) 39, 47, 97, 207, 246, 336, 348
Lsp1109I GCAGC 2 cut(s) 152, 393
MaeII ACGT 1 cut(s) 129
MalI GATC 5 cut(s) 48, 137, 246, 290, 431
MboI GATC 5 cut(s) 46, 135, 244, 288, 429
MboII GAAGA 2 cut(s) 127, 254
MflI RGATCY 2 cut(s) 288, 429
MhlI GDGCHC 1 cut(s) 300
MluCI AATT 3 cut(s) 23, 215, 324
MmeI TCCRAC 1 cut(s) 292
MnlI CCTC 3 cut(s) 58, 80, 101
MseI TTAA 1 cut(s) 213
MspI CCGG 1 cut(s) 233
MspR9I CCNGG 1 cut(s) 234
NciI CCSGG 1 cut(s) 234
NdeII GATC 5 cut(s) 46, 135, 244, 288, 429
NlaIII CATG 4 cut(s) 103, 259, 277, 358
NspI RCATGY 1 cut(s) 259
PagI TCATGA 1 cut(s) 273
PceI AGGCCT 1 cut(s) 90
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PkrI GCNGC 2 cut(s) 167, 383
PsuI RGATCY 2 cut(s) 288, 429
RsaI GTAC 1 cut(s) 254
RsaNI GTAC 1 cut(s) 253
SaqAI TTAA 1 cut(s) 213
SatI GCNGC 2 cut(s) 166, 382
Sau3AI GATC 5 cut(s) 46, 135, 244, 288, 429
ScrFI CCNGG 1 cut(s) 234
SduI GDGCHC 1 cut(s) 300
SetI ASST 6 cut(s) 55, 69, 75, 132, 167, 349
SmiI ATTTAAAT 1 cut(s) 214
Sse9I AATT 3 cut(s) 23, 215, 324
SseBI AGGCCT 1 cut(s) 90
SsiI CCGC 1 cut(s) 422
StuI AGGCCT 1 cut(s) 90
StyD4I CCNGG 1 cut(s) 232
SwaI ATTTAAAT 1 cut(s) 214
TaiI ACGT 1 cut(s) 132
TaqI TCGA 2 cut(s) 49, 138
TasI AATT 3 cut(s) 23, 215, 324
TatI WGTACW 1 cut(s) 252
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TscAI CASTG 1 cut(s) 201
TseI GCWGC 2 cut(s) 165, 381
TspDTI ATGAA 4 cut(s) 134, 219, 251, 290
TspRI CASTG 1 cut(s) 201
XapI RAATTY 1 cut(s) 23
XceI RCATGY 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.