Rorug03G0082200

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0017688
Physical Location & Seq
Forward (+)
6398364 .. 6399064
701 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0082200.1

Sequence Viewer

Length: 447 bp
ATGCTGATCGACACGGCCAAGGGAGGCGATCGTGTCGGCCACGAAGATGAGTCTTCTAATTCATTGACTCAAGCCAGAGCTCTTCTCTGCATTCTTGCACCTTCCTACTCTCAAACTCCACAAGCATTATCCCAGATTCCCAGCTTCTCTAATTATTTTTGGCTCATTTCTTATTCGCCTAATCGAGTAATGGCATCCCTGACTTCGTCGCGGAATTCCAACTCGTCCTGGACGCTGAAGCAGAACAAGCTGTTTGAGAATGCCCTGGCTGTGTACGACAAGGACACACAGGACCGGTGGCACAATTTGGCCAAGGCTGTGGGTGGAAAATCAGCCGAGGAAGTCAAGAGGCACTATGAGATTCTGGTGCAGGATCTCATGCATATAGAATCTGGTCAAGTCGTTCTACCCAATTACAGGGCCACTGGAAGCAACTCTAGACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

148

Amino Acids

16.53

Weight (kDa)

7.88

Isoelectric Point (pI)

60.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017688)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g13620
malus_domestica MD12G1142700.v1.1
prunus_persica Prupe.6G256400_v2.0.a1
rosa_laevigata RLG00000024571
rosa_multiflora Rmu_sc0003332.1_g000022
rosa_roxburghii Rroxscaffold_6G00414440
rosa_rugosa Rorug03G0082100 Rorug03G0082200
rosa_samantha Rh3AG128800 Rh3BG150100 Rh3DG150800
rosa_wichuraiana Rw3G012180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 211
AciI CCGC 1 cut(s) 211
AclWI GGATC 1 cut(s) 381
AcoI YGGCCR 3 cut(s) 15, 37, 309
AcsI RAATTY 1 cut(s) 214
AcuI CTGAAG 1 cut(s) 257
AfaI GTAC 1 cut(s) 275
AfiI CCNNNNNNNGG 1 cut(s) 417
AgeI ACCGGT 1 cut(s) 294
AjnI CCWGG 2 cut(s) 227, 264
AluBI AGCT 3 cut(s) 80, 144, 250
AluI AGCT 3 cut(s) 80, 144, 250
Alw21I GWGCWC 1 cut(s) 82
AlwI GGATC 1 cut(s) 381
AoxI GGCC 4 cut(s) 15, 37, 309, 420
ApoI RAATTY 1 cut(s) 214
AsiGI ACCGGT 1 cut(s) 294
AspS9I GGNCC 2 cut(s) 292, 420
AvaII GGWCC 1 cut(s) 292
BalI TGGCCA 1 cut(s) 311
BanII GRGCYC 1 cut(s) 82
BbsI GAAGAC 1 cut(s) 45
Bbv12I GWGCWC 1 cut(s) 82
BceAI ACGGC 1 cut(s) 30
BciT130I CCWGG 2 cut(s) 229, 266
BfaI CTAG 2 cut(s) 438, 445
Bme1390I CCNGG 2 cut(s) 229, 266
Bme18I GGWCC 1 cut(s) 292
BmgT120I GGNCC 2 cut(s) 292, 420
BmrFI CCNGG 2 cut(s) 229, 266
BmsI GCATC 1 cut(s) 203
BpiI GAAGAC 1 cut(s) 45
BplI GAGNNNNNCTC 2 cut(s) 69, 101
BpuEI CTTGAG 1 cut(s) 54
BsaJI CCNNGG 4 cut(s) 18, 264, 312, 336
BsaWI WCCGGW 1 cut(s) 294
Bsc4I CCNNNNNNNGG 1 cut(s) 417
Bse118I RCCGGY 1 cut(s) 294
Bse1I ACTGG 1 cut(s) 430
BseBI CCWGG 2 cut(s) 229, 266
BseDI CCNNGG 4 cut(s) 18, 264, 312, 336
BseGI GGATG 1 cut(s) 194
BseLI CCNNNNNNNGG 1 cut(s) 417
BseNI ACTGG 1 cut(s) 430
BseYI CCCAGC 1 cut(s) 140
BsgI GTGCAG 1 cut(s) 389
Bsh1236I CGCG 1 cut(s) 211
Bsh1285I CGRYCG 1 cut(s) 31
BshFI GGCC 4 cut(s) 17, 39, 311, 422
BshTI ACCGGT 1 cut(s) 294
BsiEI CGRYCG 1 cut(s) 31
BsiHKAI GWGCWC 1 cut(s) 82
BsiSI CCGG 1 cut(s) 295
BslI CCNNNNNNNGG 1 cut(s) 417
BsmI GAATGC 2 cut(s) 90, 265
BsnI GGCC 4 cut(s) 17, 39, 311, 422
Bsp1286I GDGCHC 1 cut(s) 82
Bsp143I GATC 3 cut(s) 6, 28, 373
BspACI CCGC 1 cut(s) 211
BspANI GGCC 4 cut(s) 17, 39, 311, 422
BspFNI CGCG 1 cut(s) 211
BspPI GGATC 1 cut(s) 381
BspQI GCTCTTC 1 cut(s) 87
BsrFI RCCGGY 1 cut(s) 294
BsrI ACTGG 1 cut(s) 430
BssAI RCCGGY 1 cut(s) 294
BssECI CCNNGG 4 cut(s) 18, 264, 312, 336
BssMI GATC 3 cut(s) 6, 28, 373
BssT1I CCWWGG 2 cut(s) 18, 312
Bst2UI CCWGG 2 cut(s) 229, 266
Bst6I CTCTTC 1 cut(s) 87
BstF5I GGATG 1 cut(s) 194
BstFNI CGCG 1 cut(s) 211
BstKTI GATC 3 cut(s) 9, 31, 376
BstMBI GATC 3 cut(s) 6, 28, 373
BstMCI CGRYCG 1 cut(s) 31
BstMWI GCNNNNNNNGC 1 cut(s) 247
BstNI CCWGG 2 cut(s) 229, 266
BstSCI CCNGG 2 cut(s) 227, 264
BstUI CGCG 1 cut(s) 211
BstV2I GAAGAC 1 cut(s) 45
BstX2I RGATCY 1 cut(s) 373
BstXI CCANNNNNNTGG 1 cut(s) 319
BstYI RGATCY 1 cut(s) 373
BsuRI GGCC 4 cut(s) 17, 39, 311, 422
BtsCI GGATG 1 cut(s) 194
BtsIMutI CAGTG 1 cut(s) 423
Cfr10I RCCGGY 1 cut(s) 294
Cfr13I GGNCC 2 cut(s) 292, 420
CseI GACGC 1 cut(s) 241
Csp6I GTAC 1 cut(s) 274
CspAI ACCGGT 1 cut(s) 294
CviAII CATG 1 cut(s) 379
CviQI GTAC 1 cut(s) 274
DpnI GATC 3 cut(s) 8, 30, 375
DpnII GATC 3 cut(s) 6, 28, 373
EaeI YGGCCR 3 cut(s) 15, 37, 309
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
Ecl136II GAGCTC 1 cut(s) 80
Eco130I CCWWGG 2 cut(s) 18, 312
Eco24I GRGCYC 1 cut(s) 82
Eco47I GGWCC 1 cut(s) 292
Eco53kI GAGCTC 1 cut(s) 80
Eco57I CTGAAG 1 cut(s) 257
EcoICRI GAGCTC 1 cut(s) 80
EcoRI GAATTC 1 cut(s) 214
EcoRII CCWGG 2 cut(s) 227, 264
EcoT14I CCWWGG 2 cut(s) 18, 312
EcoT22I ATGCAT 1 cut(s) 384
EcoT38I GRGCYC 1 cut(s) 82
ErhI CCWWGG 2 cut(s) 18, 312
FaeI CATG 1 cut(s) 382
FaiI YATR 4 cut(s) 357, 380, 384, 386
FatI CATG 1 cut(s) 378
FokI GGATG 1 cut(s) 181
FriOI GRGCYC 1 cut(s) 82
FspBI CTAG 2 cut(s) 438, 445
GsaI CCCAGC 1 cut(s) 144
HaeIII GGCC 4 cut(s) 17, 39, 311, 422
HapII CCGG 1 cut(s) 295
HgaI GACGC 1 cut(s) 241
Hin1II CATG 1 cut(s) 382
HinfI GANTC 6 cut(s) 50, 67, 136, 361, 389, 441
HpaII CCGG 1 cut(s) 295
Hpy166II GTNNAC 1 cut(s) 274
Hpy188III TCNNGA 2 cut(s) 346, 438
Hpy8I GTNNAC 1 cut(s) 274
Hpy99I CGWCG 1 cut(s) 211
HpyAV CCTTC 1 cut(s) 111
HpyCH4V TGCA 4 cut(s) 90, 98, 370, 382
HpyF10VI GCNNNNNNNGC 1 cut(s) 247
Hsp92II CATG 1 cut(s) 382
Kzo9I GATC 3 cut(s) 6, 28, 373
LguI GCTCTTC 1 cut(s) 87
LweI GCATC 1 cut(s) 203
MaeI CTAG 2 cut(s) 438, 445
MalI GATC 3 cut(s) 8, 30, 375
MboI GATC 3 cut(s) 6, 28, 373
MboII GAAGA 3 cut(s) 45, 56, 74
MflI RGATCY 1 cut(s) 373
MhlI GDGCHC 1 cut(s) 82
MlsI TGGCCA 1 cut(s) 311
MluCI AATT 5 cut(s) 58, 151, 214, 304, 412
MluNI TGGCCA 1 cut(s) 311
MlyI GAGTC 3 cut(s) 59, 61, 435
MmeI TCCRAC 1 cut(s) 243
MnlI CCTC 3 cut(s) 17, 331, 342
Mox20I TGGCCA 1 cut(s) 311
Mph1103I ATGCAT 1 cut(s) 384
MscI TGGCCA 1 cut(s) 311
MslI CAYNNNNRTG 1 cut(s) 45
Msp20I TGGCCA 1 cut(s) 311
MspI CCGG 1 cut(s) 295
MspR9I CCNGG 2 cut(s) 229, 266
Mva1269I GAATGC 2 cut(s) 90, 265
MvaI CCWGG 2 cut(s) 229, 266
MvnI CGCG 1 cut(s) 211
MwoI GCNNNNNNNGC 1 cut(s) 247
NdeII GATC 3 cut(s) 6, 28, 373
NlaIII CATG 1 cut(s) 382
NmeAIII GCCGAG 1 cut(s) 361
NsiI ATGCAT 1 cut(s) 384
PciSI GCTCTTC 1 cut(s) 87
PctI GAATGC 2 cut(s) 90, 265
PfeI GAWTC 3 cut(s) 136, 361, 389
PflFI GACNNNGTC 1 cut(s) 205
PfoI TCCNGGA 1 cut(s) 227
PinAI ACCGGT 1 cut(s) 294
Ple19I CGATCG 1 cut(s) 31
PleI GAGTC 3 cut(s) 58, 61, 435
PpsI GAGTC 3 cut(s) 58, 61, 435
Psp124BI GAGCTC 1 cut(s) 82
Psp6I CCWGG 2 cut(s) 227, 264
PspFI CCCAGC 1 cut(s) 140
PspGI CCWGG 2 cut(s) 227, 264
PspPI GGNCC 2 cut(s) 292, 420
PsuI RGATCY 1 cut(s) 373
PsyI GACNNNGTC 1 cut(s) 205
PvuI CGATCG 1 cut(s) 31
RsaI GTAC 1 cut(s) 275
RsaNI GTAC 1 cut(s) 274
RseI CAYNNNNRTG 1 cut(s) 45
SacI GAGCTC 1 cut(s) 82
SapI GCTCTTC 1 cut(s) 87
Sau3AI GATC 3 cut(s) 6, 28, 373
Sau96I GGNCC 2 cut(s) 292, 420
SchI GAGTC 3 cut(s) 59, 61, 435
ScrFI CCNGG 2 cut(s) 229, 266
SduI GDGCHC 1 cut(s) 82
SetI ASST 4 cut(s) 82, 103, 146, 252
SfaNI GCATC 1 cut(s) 203
SinI GGWCC 1 cut(s) 292
SmiMI CAYNNNNRTG 1 cut(s) 45
SmlI CTYRAG 1 cut(s) 69
SmoI CTYRAG 1 cut(s) 69
Sse9I AATT 5 cut(s) 58, 151, 214, 304, 412
SsiI CCGC 1 cut(s) 211
SspMI CTAG 2 cut(s) 438, 445
SstI GAGCTC 1 cut(s) 82
StyD4I CCNGG 2 cut(s) 227, 264
StyI CCWWGG 2 cut(s) 18, 312
TaqI TCGA 2 cut(s) 9, 184
TasI AATT 5 cut(s) 58, 151, 214, 304, 412
TfiI GAWTC 3 cut(s) 136, 361, 389
TscAI CASTG 1 cut(s) 430
TspDTI ATGAA 1 cut(s) 51
TspRI CASTG 1 cut(s) 430
Tth111I GACNNNGTC 1 cut(s) 205
VpaK11BI GGWCC 1 cut(s) 292
XapI RAATTY 1 cut(s) 214
XbaI TCTAGA 1 cut(s) 437
XspI CTAG 2 cut(s) 438, 445
Zsp2I ATGCAT 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.