Rw3G012180

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0017688
Physical Location & Seq
Reverse (-)
11975984 .. 11976798
815 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G012180.1

Sequence Viewer

Length: 486 bp
ATGTTTCTAATCAAGTCTTTAAATCACTCATCAAAGAGAGAGTTTGGAGAGATAGAGATGGGAGACAGAGCTGGTGTACTGGAGGTGAAGCTGATTGAAGCAAGAGACCTCAAAAACATGGATTTTGCTGTGAAGATGAACACATACGTTGTGATCGAATATGGAAATCAAAAGCATACAAGCTGCGTTGCTAAAGGAAAAGGAACGAAACCAGTGTGGAATGAAACATTTAGATTCGAGGCGGAGTATCCGGGTGGGGATGAAAATCACAAGTACATGCTTCATTTTCGTATCATGAATACACATAAGATCTTGGACCACGACGTATTTGTTGGCCAATCTACAATTTATGTGAAGGATTTGATAGCTTTGGGCACGGACAGAGGGAATGCTGAGCTTCGAAGCGCCAAGTATAGAGTTGTTCTTCAAGACAAGACTTACGCCGGAGAGATCTCTGTTGCTTTATCTTTTTCCATAAACCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

161

Amino Acids

18.37

Weight (kDa)

7.85

Isoelectric Point (pI)

34.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 25 - 121 4.4e-18 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017688)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g13620
malus_domestica MD12G1142700.v1.1
prunus_persica Prupe.6G256400_v2.0.a1
rosa_laevigata RLG00000024571
rosa_multiflora Rmu_sc0003332.1_g000022
rosa_roxburghii Rroxscaffold_6G00414440
rosa_rugosa Rorug03G0082100 Rorug03G0082200
rosa_samantha Rh3AG128800 Rh3BG150100 Rh3DG150800
rosa_wichuraiana Rw3G012180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 242
AcoI YGGCCR 1 cut(s) 334
AfaI GTAC 2 cut(s) 78, 275
AgsI TTSAA 2 cut(s) 98, 428
AluBI AGCT 5 cut(s) 71, 91, 183, 368, 397
AluI AGCT 5 cut(s) 71, 91, 183, 368, 397
Alw26I GTCTC 2 cut(s) 57, 99
AoxI GGCC 1 cut(s) 334
ApeKI GCWGC 1 cut(s) 183
AspLEI GCGC 1 cut(s) 407
AspS9I GGNCC 1 cut(s) 316
AsuC2I CCSGG 1 cut(s) 252
AsuHPI GGTGA 1 cut(s) 97
AsuII TTCGAA 1 cut(s) 400
AvaII GGWCC 1 cut(s) 316
BaeGI GKGCMC 1 cut(s) 377
BalI TGGCCA 1 cut(s) 336
BbvI GCAGC 1 cut(s) 170
BccI CCATC 1 cut(s) 52
BciVI GTATCC 1 cut(s) 258
BcnI CCSGG 1 cut(s) 252
BcoDI GTCTC 2 cut(s) 57, 99
BfoI RGCGCY 1 cut(s) 408
BfuI GTATCC 1 cut(s) 258
BglII AGATCT 2 cut(s) 309, 450
BisI GCNGC 1 cut(s) 184
BlpI GCTNAGC 1 cut(s) 393
BlsI GCNGC 1 cut(s) 185
Bme1390I CCNGG 1 cut(s) 252
Bme18I GGWCC 1 cut(s) 316
BmgT120I GGNCC 1 cut(s) 316
BmrFI CCNGG 1 cut(s) 252
BpmI CTGGAG 1 cut(s) 101
Bpu1102I GCTNAGC 1 cut(s) 393
Bpu14I TTCGAA 1 cut(s) 400
BpuMI CCSGG 1 cut(s) 252
BsaBI GATNNNNATC 1 cut(s) 264
BsaI GGTCTC 1 cut(s) 99
Bse1I ACTGG 2 cut(s) 84, 212
Bse8I GATNNNNATC 1 cut(s) 264
BseGI GGATG 1 cut(s) 265
BseJI GATNNNNATC 1 cut(s) 264
BseMII CTCAG 1 cut(s) 384
BseNI ACTGG 2 cut(s) 84, 212
BseSI GKGCMC 1 cut(s) 377
BseXI GCAGC 1 cut(s) 170
BshFI GGCC 1 cut(s) 336
BsiSI CCGG 2 cut(s) 251, 444
BsmAI GTCTC 2 cut(s) 57, 99
BsmI GAATGC 1 cut(s) 394
BsnI GGCC 1 cut(s) 336
Bso31I GGTCTC 1 cut(s) 99
Bsp119I TTCGAA 1 cut(s) 400
Bsp1286I GDGCHC 1 cut(s) 377
Bsp143I GATC 3 cut(s) 153, 309, 450
Bsp1720I GCTNAGC 1 cut(s) 393
BspACI CCGC 1 cut(s) 242
BspANI GGCC 1 cut(s) 336
BspCNI CTCAG 1 cut(s) 385
BspHI TCATGA 1 cut(s) 294
BspT104I TTCGAA 1 cut(s) 400
BspTNI GGTCTC 1 cut(s) 99
BsrI ACTGG 2 cut(s) 84, 212
BssMI GATC 3 cut(s) 153, 309, 450
BstBI TTCGAA 1 cut(s) 400
BstDEI CTNAG 1 cut(s) 393
BstF5I GGATG 1 cut(s) 265
BstH2I RGCGCY 1 cut(s) 408
BstHHI GCGC 1 cut(s) 407
BstKTI GATC 3 cut(s) 156, 312, 453
BstMAI GTCTC 2 cut(s) 57, 99
BstMBI GATC 3 cut(s) 153, 309, 450
BstNSI RCATGY 1 cut(s) 280
BstSCI CCNGG 1 cut(s) 250
BstSLI GKGCMC 1 cut(s) 377
BstV1I GCAGC 1 cut(s) 170
BstX2I RGATCY 2 cut(s) 309, 450
BstYI RGATCY 2 cut(s) 309, 450
BsuI GTATCC 1 cut(s) 258
BsuRI GGCC 1 cut(s) 336
BtsCI GGATG 1 cut(s) 265
BtsIMutI CAGTG 1 cut(s) 219
CciI TCATGA 1 cut(s) 294
CfoI GCGC 1 cut(s) 407
Cfr13I GGNCC 1 cut(s) 316
Csp6I GTAC 2 cut(s) 77, 274
CviAII CATG 3 cut(s) 118, 277, 295
CviJI RGCY 6 cut(s) 71, 91, 183, 336, 368, 397
CviKI_1 RGCY 6 cut(s) 71, 91, 183, 336, 368, 397
CviQI GTAC 2 cut(s) 77, 274
DdeI CTNAG 1 cut(s) 393
DpnI GATC 3 cut(s) 155, 311, 452
DpnII GATC 3 cut(s) 153, 309, 450
DraI TTTAAA 1 cut(s) 21
EaeI YGGCCR 1 cut(s) 334
EciI GGCGGA 1 cut(s) 257
Eco31I GGTCTC 1 cut(s) 99
Eco47I GGWCC 1 cut(s) 316
FaeI CATG 3 cut(s) 121, 280, 298
FatI CATG 3 cut(s) 117, 276, 294
Fnu4HI GCNGC 1 cut(s) 184
FokI GGATG 1 cut(s) 272
Fsp4HI GCNGC 1 cut(s) 184
GlaI GCGC 1 cut(s) 406
GluI GCNGC 1 cut(s) 184
GsuI CTGGAG 1 cut(s) 101
HaeII RGCGCY 1 cut(s) 408
HaeIII GGCC 1 cut(s) 336
HapII CCGG 2 cut(s) 251, 444
HhaI GCGC 1 cut(s) 407
Hin1II CATG 3 cut(s) 121, 280, 298
Hin6I GCGC 1 cut(s) 405
HinP1I GCGC 1 cut(s) 405
HinfI GANTC 1 cut(s) 234
HpaII CCGG 2 cut(s) 251, 444
HphI GGTGA 1 cut(s) 97
Hpy166II GTNNAC 1 cut(s) 77
Hpy188III TCNNGA 2 cut(s) 295, 428
Hpy8I GTNNAC 1 cut(s) 77
Hpy99I CGWCG 1 cut(s) 326
HpyAV CCTTC 1 cut(s) 349
HpyCH4IV ACGT 2 cut(s) 147, 324
HpyF3I CTNAG 1 cut(s) 393
HpySE526I ACGT 2 cut(s) 147, 324
Hsp92II CATG 3 cut(s) 121, 280, 298
HspAI GCGC 1 cut(s) 405
Kzo9I GATC 3 cut(s) 153, 309, 450
LpnPI CCDG 5 cut(s) 57, 65, 225, 264, 457
Lsp1109I GCAGC 1 cut(s) 170
MaeII ACGT 2 cut(s) 147, 324
MalI GATC 3 cut(s) 155, 311, 452
MboI GATC 3 cut(s) 153, 309, 450
MboII GAAGA 2 cut(s) 145, 416
MflI RGATCY 2 cut(s) 309, 450
MhlI GDGCHC 1 cut(s) 377
MlsI TGGCCA 1 cut(s) 336
MluCI AATT 1 cut(s) 345
MluNI TGGCCA 1 cut(s) 336
MnlI CCTC 4 cut(s) 76, 119, 232, 377
Mox20I TGGCCA 1 cut(s) 336
MscI TGGCCA 1 cut(s) 336
MseI TTAA 1 cut(s) 20
Msp20I TGGCCA 1 cut(s) 336
MspI CCGG 2 cut(s) 251, 444
MspR9I CCNGG 1 cut(s) 252
Mva1269I GAATGC 1 cut(s) 394
NciI CCSGG 1 cut(s) 252
NdeII GATC 3 cut(s) 153, 309, 450
NlaIII CATG 3 cut(s) 121, 280, 298
NspI RCATGY 1 cut(s) 280
NspV TTCGAA 1 cut(s) 400
PagI TCATGA 1 cut(s) 294
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PctI GAATGC 1 cut(s) 394
PfeI GAWTC 1 cut(s) 234
PkrI GCNGC 1 cut(s) 185
PspPI GGNCC 1 cut(s) 316
PsuI RGATCY 2 cut(s) 309, 450
RsaI GTAC 2 cut(s) 78, 275
RsaNI GTAC 2 cut(s) 77, 274
SaqAI TTAA 1 cut(s) 20
SatI GCNGC 1 cut(s) 184
Sau3AI GATC 3 cut(s) 153, 309, 450
Sau96I GGNCC 1 cut(s) 316
ScrFI CCNGG 1 cut(s) 252
SduI GDGCHC 1 cut(s) 377
SetI ASST 9 cut(s) 73, 87, 93, 111, 150, 185, 327, 370, 399
SfuI TTCGAA 1 cut(s) 400
SinI GGWCC 1 cut(s) 316
Sse9I AATT 1 cut(s) 345
SsiI CCGC 1 cut(s) 242
StyD4I CCNGG 1 cut(s) 250
TaiI ACGT 2 cut(s) 150, 327
TaqI TCGA 3 cut(s) 156, 237, 400
TasI AATT 1 cut(s) 345
TatI WGTACW 2 cut(s) 76, 273
TfiI GAWTC 1 cut(s) 234
Tru1I TTAA 1 cut(s) 20
Tru9I TTAA 1 cut(s) 20
TscAI CASTG 1 cut(s) 219
TseI GCWGC 1 cut(s) 183
TspDTI ATGAA 5 cut(s) 152, 237, 272, 276, 311
TspGWI ACGGA 1 cut(s) 392
TspRI CASTG 1 cut(s) 219
VpaK11BI GGWCC 1 cut(s) 316
XceI RCATGY 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.