MD12G1142700.v1.1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0017688
Physical Location & Seq
Forward (+)
22193154 .. 22194061
908 bp
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UTR
Exon/CDS
Intron
MD12G1142700.v1.1.491

Sequence Viewer

Length: 420 bp
ATGGCAGGTGGTGTATTGGAGGTCACATTGGTTGAAGCTCAGGGCCTCAAGAACATGGATTTTTTTGTAAAGATGAACACGTACGTTGTAATCCAATATGGAAATCAAAAGCATACAAGCAACGTGGCCAAAGGATCAGGAACGAAACCAATATGGAATGAAAAGTTTACATTCAACGTGGAATATCCTGGAGGCGAAGATCACAAATATATGCTTTGGTTTCGTATCATGGACACCCAAAAGCTCTTGGACCATGATGTAGTTGTCGGTGAATCCAAGGTGTACGTGAAGGATGTGATAGCGTTGGGCACAGAGAGAGGAAAGGCTGAACTTCGATCTGCAAAGCACAGAGTTGTCCTTCAAGACAAGACTTATGCCGGAGAGATCTCTGTTGCTTTAACTTTCACCTTAAATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

140

Amino Acids

15.63

Weight (kDa)

7.92

Isoelectric Point (pI)

25.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 4 - 100 1.4e-18 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017688)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g13620
malus_domestica MD12G1142700.v1.1
prunus_persica Prupe.6G256400_v2.0.a1
rosa_laevigata RLG00000024571
rosa_multiflora Rmu_sc0003332.1_g000022
rosa_roxburghii Rroxscaffold_6G00414440
rosa_rugosa Rorug03G0082100 Rorug03G0082200
rosa_samantha Rh3AG128800 Rh3BG150100 Rh3DG150800
rosa_wichuraiana Rw3G012180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 142
AcoI YGGCCR 1 cut(s) 126
AcsI RAATTY 1 cut(s) 412
AfaI GTAC 2 cut(s) 83, 284
AflIII ACRYGT 1 cut(s) 78
AgsI TTSAA 3 cut(s) 35, 175, 362
AjnI CCWGG 1 cut(s) 187
AluBI AGCT 2 cut(s) 38, 244
AluI AGCT 2 cut(s) 38, 244
AlwI GGATC 1 cut(s) 142
AoxI GGCC 2 cut(s) 43, 126
ApoI RAATTY 1 cut(s) 412
AspS9I GGNCC 2 cut(s) 43, 250
AsuHPI GGTGA 2 cut(s) 281, 397
AvaII GGWCC 1 cut(s) 250
BaeGI GKGCMC 1 cut(s) 311
BalI TGGCCA 1 cut(s) 128
BciT130I CCWGG 1 cut(s) 189
BglII AGATCT 1 cut(s) 384
Bme1390I CCNGG 1 cut(s) 189
Bme18I GGWCC 1 cut(s) 250
BmgT120I GGNCC 2 cut(s) 43, 250
BmrFI CCNGG 1 cut(s) 189
BpmI CTGGAG 1 cut(s) 210
Bpu10I CCTNAGC 1 cut(s) 39
BpuEI CTTGAG 1 cut(s) 32
BsaAI YACGTR 2 cut(s) 81, 286
BsaJI CCNNGG 1 cut(s) 276
BseBI CCWGG 1 cut(s) 189
BseDI CCNNGG 1 cut(s) 276
BseGI GGATG 1 cut(s) 298
BseMII CTCAG 1 cut(s) 53
BseSI GKGCMC 1 cut(s) 311
BshFI GGCC 2 cut(s) 45, 128
BsiSI CCGG 1 cut(s) 378
BsiWI CGTACG 1 cut(s) 81
BsnI GGCC 2 cut(s) 45, 128
Bsp1286I GDGCHC 1 cut(s) 311
Bsp143I GATC 4 cut(s) 134, 199, 335, 384
BspANI GGCC 2 cut(s) 45, 128
BspCNI CTCAG 1 cut(s) 52
BspHI TCATGA 1 cut(s) 416
BspPI GGATC 1 cut(s) 142
BssECI CCNNGG 1 cut(s) 276
BssMI GATC 4 cut(s) 134, 199, 335, 384
BssT1I CCWWGG 1 cut(s) 276
Bst2UI CCWGG 1 cut(s) 189
BstBAI YACGTR 2 cut(s) 81, 286
BstDEI CTNAG 1 cut(s) 39
BstF5I GGATG 1 cut(s) 298
BstKTI GATC 4 cut(s) 137, 202, 338, 387
BstMBI GATC 4 cut(s) 134, 199, 335, 384
BstNI CCWGG 1 cut(s) 189
BstSCI CCNGG 1 cut(s) 187
BstSLI GKGCMC 1 cut(s) 311
BstX2I RGATCY 1 cut(s) 384
BstYI RGATCY 1 cut(s) 384
BsuRI GGCC 2 cut(s) 45, 128
BtsCI GGATG 1 cut(s) 298
CciI TCATGA 1 cut(s) 416
Cfr13I GGNCC 2 cut(s) 43, 250
Csp6I GTAC 2 cut(s) 82, 283
CspCI CAANNNNNGTGG 2 cut(s) 105, 140
CviAII CATG 4 cut(s) 55, 229, 254, 417
CviJI RGCY 5 cut(s) 38, 45, 128, 244, 326
CviKI_1 RGCY 5 cut(s) 38, 45, 128, 244, 326
CviQI GTAC 2 cut(s) 82, 283
DdeI CTNAG 1 cut(s) 39
DpnI GATC 4 cut(s) 136, 201, 337, 386
DpnII GATC 4 cut(s) 134, 199, 335, 384
EaeI YGGCCR 1 cut(s) 126
Eco130I CCWWGG 1 cut(s) 276
Eco47I GGWCC 1 cut(s) 250
EcoO109I RGGNCCY 1 cut(s) 43
EcoRII CCWGG 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 276
ErhI CCWWGG 1 cut(s) 276
FaeI CATG 4 cut(s) 58, 232, 257, 420
FatI CATG 4 cut(s) 54, 228, 253, 416
FokI GGATG 1 cut(s) 305
GsuI CTGGAG 1 cut(s) 210
HaeIII GGCC 2 cut(s) 45, 128
HapII CCGG 1 cut(s) 378
Hin1II CATG 4 cut(s) 58, 232, 257, 420
HinfI GANTC 1 cut(s) 272
HpaII CCGG 1 cut(s) 378
HphI GGTGA 2 cut(s) 281, 397
Hpy166II GTNNAC 2 cut(s) 168, 283
Hpy188III TCNNGA 4 cut(s) 49, 138, 362, 417
Hpy8I GTNNAC 2 cut(s) 168, 283
HpyAV CCTTC 2 cut(s) 283, 368
HpyCH4IV ACGT 5 cut(s) 80, 84, 123, 177, 285
HpyCH4V TGCA 1 cut(s) 341
HpyF3I CTNAG 1 cut(s) 39
HpySE526I ACGT 5 cut(s) 80, 84, 123, 177, 285
Hsp92II CATG 4 cut(s) 58, 232, 257, 420
Kzo9I GATC 4 cut(s) 134, 199, 335, 384
LpnPI CCDG 5 cut(s) 26, 123, 174, 201, 391
MaeII ACGT 5 cut(s) 80, 84, 123, 177, 285
MaeIII GTNAC 1 cut(s) 22
MalI GATC 4 cut(s) 136, 201, 337, 386
MboI GATC 4 cut(s) 134, 199, 335, 384
MboII GAAGA 1 cut(s) 209
MflI RGATCY 1 cut(s) 384
MhlI GDGCHC 1 cut(s) 311
MlsI TGGCCA 1 cut(s) 128
MluCI AATT 1 cut(s) 412
MluNI TGGCCA 1 cut(s) 128
MnlI CCTC 4 cut(s) 13, 56, 185, 311
Mox20I TGGCCA 1 cut(s) 128
MscI TGGCCA 1 cut(s) 128
MseI TTAA 2 cut(s) 398, 410
Msp20I TGGCCA 1 cut(s) 128
MspI CCGG 1 cut(s) 378
MspR9I CCNGG 1 cut(s) 189
MvaI CCWGG 1 cut(s) 189
NdeII GATC 4 cut(s) 134, 199, 335, 384
NlaIII CATG 4 cut(s) 58, 232, 257, 420
NmuCI GTSAC 1 cut(s) 22
PagI TCATGA 1 cut(s) 416
PfeI GAWTC 1 cut(s) 272
Pfl23II CGTACG 1 cut(s) 81
PfoI TCCNGGA 1 cut(s) 187
Ppu21I YACGTR 2 cut(s) 81, 286
Psp6I CCWGG 1 cut(s) 187
PspGI CCWGG 1 cut(s) 187
PspLI CGTACG 1 cut(s) 81
PspPI GGNCC 2 cut(s) 43, 250
PsuI RGATCY 1 cut(s) 384
RsaI GTAC 2 cut(s) 83, 284
RsaNI GTAC 2 cut(s) 82, 283
SaqAI TTAA 2 cut(s) 398, 410
Sau3AI GATC 4 cut(s) 134, 199, 335, 384
Sau96I GGNCC 2 cut(s) 43, 250
ScrFI CCNGG 1 cut(s) 189
SduI GDGCHC 1 cut(s) 311
SinI GGWCC 1 cut(s) 250
SmlI CTYRAG 1 cut(s) 47
SmoI CTYRAG 1 cut(s) 47
Sse9I AATT 1 cut(s) 412
StyD4I CCNGG 1 cut(s) 187
StyI CCWWGG 1 cut(s) 276
TaiI ACGT 5 cut(s) 83, 87, 126, 180, 288
TaqI TCGA 1 cut(s) 334
TasI AATT 1 cut(s) 412
TfiI GAWTC 1 cut(s) 272
Tru1I TTAA 2 cut(s) 398, 410
Tru9I TTAA 2 cut(s) 398, 410
TseFI GTSAC 1 cut(s) 22
Tsp45I GTSAC 1 cut(s) 22
TspDTI ATGAA 3 cut(s) 89, 174, 405
VpaK11BI GGWCC 1 cut(s) 250
XapI RAATTY 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.