Rmu_sc0003332.1_g000022

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003332.1
OG0017688
Physical Location & Seq
Forward (+)
94452 .. 95210
759 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003332.1_g000022.1.cds

Sequence Viewer

Length: 429 bp
atgggagacagagctggtgtactggaggtgaagctgattgaagcaagagacctcaaaaacatggattttgctgtgaagatgaacacatacgttgtgatcgaatatggaaatcaaaagcatacaagctgcgttgctaaaggaaaaggaacgaaaccagtgtggaatgaaacatttagattcgaggcggagtatccgggtggggatgaaaatcacaagtacatgcttcattttcgtatcatgaatacacataagatcttggaccacgacgtatttgttggccaatctacaatttatgtgaaggatttgatagctttgggcacggacagagggaatgctgagcttcgaagcgccaagtatagagttgttcttcaagacaagacttacgccggagagatttctgttgctttatctttttccataaaccaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

142

Amino Acids

16.12

Weight (kDa)

7.84

Isoelectric Point (pI)

30.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017688)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g13620
malus_domestica MD12G1142700.v1.1
prunus_persica Prupe.6G256400_v2.0.a1
rosa_laevigata RLG00000024571
rosa_multiflora Rmu_sc0003332.1_g000022
rosa_roxburghii Rroxscaffold_6G00414440
rosa_rugosa Rorug03G0082100 Rorug03G0082200
rosa_samantha Rh3AG128800 Rh3BG150100 Rh3DG150800
rosa_wichuraiana Rw3G012180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 185
AcoI YGGCCR 1 cut(s) 277
AfaI GTAC 2 cut(s) 21, 218
AgsI TTSAA 2 cut(s) 41, 371
AluBI AGCT 5 cut(s) 14, 34, 126, 311, 340
AluI AGCT 5 cut(s) 14, 34, 126, 311, 340
Alw26I GTCTC 1 cut(s) 42
AoxI GGCC 1 cut(s) 277
ApeKI GCWGC 1 cut(s) 126
AspLEI GCGC 1 cut(s) 350
AspS9I GGNCC 1 cut(s) 259
AsuC2I CCSGG 1 cut(s) 195
AsuHPI GGTGA 1 cut(s) 40
AsuII TTCGAA 1 cut(s) 343
AvaII GGWCC 1 cut(s) 259
BaeGI GKGCMC 1 cut(s) 320
BalI TGGCCA 1 cut(s) 279
BbvI GCAGC 1 cut(s) 113
BciVI GTATCC 1 cut(s) 201
BcnI CCSGG 1 cut(s) 195
BcoDI GTCTC 1 cut(s) 42
BfoI RGCGCY 1 cut(s) 351
BfuI GTATCC 1 cut(s) 201
BglII AGATCT 1 cut(s) 252
BisI GCNGC 1 cut(s) 127
BlpI GCTNAGC 1 cut(s) 336
BlsI GCNGC 1 cut(s) 128
Bme1390I CCNGG 1 cut(s) 195
Bme18I GGWCC 1 cut(s) 259
BmgT120I GGNCC 1 cut(s) 259
BmrFI CCNGG 1 cut(s) 195
BpmI CTGGAG 1 cut(s) 44
Bpu1102I GCTNAGC 1 cut(s) 336
Bpu14I TTCGAA 1 cut(s) 343
BpuMI CCSGG 1 cut(s) 195
BsaBI GATNNNNATC 1 cut(s) 207
BsaI GGTCTC 1 cut(s) 42
Bse1I ACTGG 2 cut(s) 27, 155
Bse8I GATNNNNATC 1 cut(s) 207
BseGI GGATG 1 cut(s) 208
BseJI GATNNNNATC 1 cut(s) 207
BseMII CTCAG 1 cut(s) 327
BseNI ACTGG 2 cut(s) 27, 155
BseSI GKGCMC 1 cut(s) 320
BseXI GCAGC 1 cut(s) 113
BshFI GGCC 1 cut(s) 279
BsiSI CCGG 2 cut(s) 194, 387
BsmAI GTCTC 1 cut(s) 42
BsmI GAATGC 1 cut(s) 337
BsnI GGCC 1 cut(s) 279
Bso31I GGTCTC 1 cut(s) 42
Bsp119I TTCGAA 1 cut(s) 343
Bsp1286I GDGCHC 1 cut(s) 320
Bsp143I GATC 2 cut(s) 96, 252
Bsp1720I GCTNAGC 1 cut(s) 336
BspACI CCGC 1 cut(s) 185
BspANI GGCC 1 cut(s) 279
BspCNI CTCAG 1 cut(s) 328
BspHI TCATGA 1 cut(s) 237
BspT104I TTCGAA 1 cut(s) 343
BspTNI GGTCTC 1 cut(s) 42
BsrI ACTGG 2 cut(s) 27, 155
BssMI GATC 2 cut(s) 96, 252
BstBI TTCGAA 1 cut(s) 343
BstDEI CTNAG 1 cut(s) 336
BstF5I GGATG 1 cut(s) 208
BstH2I RGCGCY 1 cut(s) 351
BstHHI GCGC 1 cut(s) 350
BstKTI GATC 2 cut(s) 99, 255
BstMAI GTCTC 1 cut(s) 42
BstMBI GATC 2 cut(s) 96, 252
BstNSI RCATGY 1 cut(s) 223
BstSCI CCNGG 1 cut(s) 193
BstSLI GKGCMC 1 cut(s) 320
BstV1I GCAGC 1 cut(s) 113
BstX2I RGATCY 1 cut(s) 252
BstYI RGATCY 1 cut(s) 252
BsuI GTATCC 1 cut(s) 201
BsuRI GGCC 1 cut(s) 279
BtsCI GGATG 1 cut(s) 208
BtsIMutI CAGTG 1 cut(s) 162
CciI TCATGA 1 cut(s) 237
CfoI GCGC 1 cut(s) 350
Cfr13I GGNCC 1 cut(s) 259
Csp6I GTAC 2 cut(s) 20, 217
CviAII CATG 3 cut(s) 61, 220, 238
CviJI RGCY 6 cut(s) 14, 34, 126, 279, 311, 340
CviKI_1 RGCY 6 cut(s) 14, 34, 126, 279, 311, 340
CviQI GTAC 2 cut(s) 20, 217
DdeI CTNAG 1 cut(s) 336
DpnI GATC 2 cut(s) 98, 254
DpnII GATC 2 cut(s) 96, 252
EaeI YGGCCR 1 cut(s) 277
EciI GGCGGA 1 cut(s) 200
Eco31I GGTCTC 1 cut(s) 42
Eco47I GGWCC 1 cut(s) 259
FaeI CATG 3 cut(s) 64, 223, 241
FatI CATG 3 cut(s) 60, 219, 237
Fnu4HI GCNGC 1 cut(s) 127
FokI GGATG 1 cut(s) 215
Fsp4HI GCNGC 1 cut(s) 127
GlaI GCGC 1 cut(s) 349
GluI GCNGC 1 cut(s) 127
GsuI CTGGAG 1 cut(s) 44
HaeII RGCGCY 1 cut(s) 351
HaeIII GGCC 1 cut(s) 279
HapII CCGG 2 cut(s) 194, 387
HhaI GCGC 1 cut(s) 350
Hin1II CATG 3 cut(s) 64, 223, 241
Hin6I GCGC 1 cut(s) 348
HinP1I GCGC 1 cut(s) 348
HinfI GANTC 1 cut(s) 177
HpaII CCGG 2 cut(s) 194, 387
HphI GGTGA 1 cut(s) 40
Hpy166II GTNNAC 1 cut(s) 20
Hpy188III TCNNGA 2 cut(s) 238, 371
Hpy8I GTNNAC 1 cut(s) 20
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 1 cut(s) 292
HpyCH4IV ACGT 2 cut(s) 90, 267
HpyF3I CTNAG 1 cut(s) 336
HpySE526I ACGT 2 cut(s) 90, 267
Hsp92II CATG 3 cut(s) 64, 223, 241
HspAI GCGC 1 cut(s) 348
Kzo9I GATC 2 cut(s) 96, 252
LpnPI CCDG 4 cut(s) 8, 168, 207, 400
Lsp1109I GCAGC 1 cut(s) 113
MaeII ACGT 2 cut(s) 90, 267
MalI GATC 2 cut(s) 98, 254
MboI GATC 2 cut(s) 96, 252
MboII GAAGA 2 cut(s) 88, 359
MflI RGATCY 1 cut(s) 252
MhlI GDGCHC 1 cut(s) 320
MlsI TGGCCA 1 cut(s) 279
MluCI AATT 1 cut(s) 288
MluNI TGGCCA 1 cut(s) 279
MnlI CCTC 4 cut(s) 19, 62, 175, 320
Mox20I TGGCCA 1 cut(s) 279
MscI TGGCCA 1 cut(s) 279
Msp20I TGGCCA 1 cut(s) 279
MspI CCGG 2 cut(s) 194, 387
MspR9I CCNGG 1 cut(s) 195
Mva1269I GAATGC 1 cut(s) 337
NciI CCSGG 1 cut(s) 195
NdeII GATC 2 cut(s) 96, 252
NlaIII CATG 3 cut(s) 64, 223, 241
NspI RCATGY 1 cut(s) 223
NspV TTCGAA 1 cut(s) 343
PagI TCATGA 1 cut(s) 237
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PctI GAATGC 1 cut(s) 337
PfeI GAWTC 1 cut(s) 177
PkrI GCNGC 1 cut(s) 128
PspPI GGNCC 1 cut(s) 259
PsuI RGATCY 1 cut(s) 252
RsaI GTAC 2 cut(s) 21, 218
RsaNI GTAC 2 cut(s) 20, 217
SatI GCNGC 1 cut(s) 127
Sau3AI GATC 2 cut(s) 96, 252
Sau96I GGNCC 1 cut(s) 259
ScrFI CCNGG 1 cut(s) 195
SduI GDGCHC 1 cut(s) 320
SetI ASST 9 cut(s) 16, 30, 36, 54, 93, 128, 270, 313, 342
SfuI TTCGAA 1 cut(s) 343
SinI GGWCC 1 cut(s) 259
Sse9I AATT 1 cut(s) 288
SsiI CCGC 1 cut(s) 185
StyD4I CCNGG 1 cut(s) 193
TaiI ACGT 2 cut(s) 93, 270
TaqI TCGA 3 cut(s) 99, 180, 343
TasI AATT 1 cut(s) 288
TatI WGTACW 2 cut(s) 19, 216
TfiI GAWTC 1 cut(s) 177
TscAI CASTG 1 cut(s) 162
TseI GCWGC 1 cut(s) 126
TspDTI ATGAA 5 cut(s) 95, 180, 215, 219, 254
TspGWI ACGGA 1 cut(s) 335
TspRI CASTG 1 cut(s) 162
VpaK11BI GGWCC 1 cut(s) 259
XceI RCATGY 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.