FvH4_6g14520

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0017139
Physical Location & Seq
Forward (+)
8925867 .. 8927234
1368 bp
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UTR
Exon/CDS
Intron
FvH4_6g14520.t1

Sequence Viewer

Length: 870 bp
ATGGTTTACATTTGGAGTGATCAAGAGACTGTTGATGAGCTGAAATACAAGCTTTTCTCCATCAATTATGAACTAGAATCAGCGAAAATAAGGGCGAGCCACGAGATAAGCAAAAATGAGGAGATTACAAAGCAGTTGTTTCATCTCTTGAAGGCAGCTTGTCGAGAAAGAGATGAAGCGAAAGACCAGTTGCAAAACCTACTCAAAATGTTCTTGCCCATACCAGCTGAAAATGAAACAAGCCCTATATTCTCTGATTTCAACAATGAAAATAATCAGATTCTGCAAAACGCCTCAGAATTCTCAGCAGTTAAAGAACCAGATGTTATATCCAAAACTAGTTCATATCCCACTATACGCAACTTTGCTGTTGATTCAAGTAACTCAGGATTTCCCAAGAATGCTGCTGTTCAAGAATGTGATGAGTCCATTCCGGTGGCTACAATGTCTTCATCGAACTCAAAGAAAACTGATCTCGCTGATGTTTTGATTGATAATATTGTTAGGGTCAGACCACTGCCTAAGAAAGGAAGATTGTTCCAGGCTGTATTTGAAAATGAGCCTTTACTTCAGACACTTCTTGTTGCTCCTCTTCCCAAGTGGAAAAACCCTCCACCTATTCCTCCTTCAAAAAGTTCATCCAATCTCATTTATGGATGTGACTTGCCCACAGAAGAACAGATTGAAGCAGTCAGTCCGATTCGTGCAATACAAGAATCCAGCTCACCCAGACTTTCTCCTATACCATCTTCATCTGTGTTGAACTTTGCACATGGTTCTGCAAGTATGAAGACTGGAGTTGATAACAATTCAAGTGATCTCATGCATAGTCAGATTGGAACCTCTAAGAGAAGGAATAAATGGTTATGA

Protein Analysis

290

Amino Acids

32.26

Weight (kDa)

5.71

Isoelectric Point (pI)

58.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1635 PF07795 10 - 208 1e-28 Protein of unknown function (DUF1635)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017139)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09940
fragaria_vesca FvH4_6g14520
malus_domestica MD12G1136600.v1.1
rosa_chinensis RchiOBHm_Chr3g0467601
rosa_laevigata RLG00000024486
rosa_multiflora Rmu_sc0011632.1_g000011
rosa_roxburghii Rroxscaffold_6G00413560
rosa_rugosa Rorug03G0088400
rosa_samantha Rh3AG136700 Rh3BG158500 Rh3CG158200 Rh3DG159000
rosa_wichuraiana Rw3G012950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 299
AcuI CTGAAG 1 cut(s) 554
AfiI CCNNNNNNNGG 1 cut(s) 527
AgsI TTSAA 9 cut(s) 151, 262, 378, 413, 554, 630, 686, 763, 813
AhlI ACTAGT 1 cut(s) 338
AjnI CCWGG 1 cut(s) 540
AluBI AGCT 5 cut(s) 40, 52, 158, 227, 723
AluI AGCT 5 cut(s) 40, 52, 158, 227, 723
Alw26I GTCTC 1 cut(s) 20
AlwNI CAGNNNCTG 1 cut(s) 283
ApeKI GCWGC 2 cut(s) 155, 404
ApoI RAATTY 1 cut(s) 299
AsuHPI GGTGA 1 cut(s) 717
BauI CACGAG 1 cut(s) 101
BbsI GAAGAC 2 cut(s) 441, 797
BbvI GCAGC 2 cut(s) 167, 391
BccI CCATC 2 cut(s) 68, 754
BciT130I CCWGG 1 cut(s) 542
BclI TGATCA 1 cut(s) 19
BcoDI GTCTC 1 cut(s) 20
BcuI ACTAGT 1 cut(s) 338
BfaI CTAG 2 cut(s) 74, 339
BisI GCNGC 2 cut(s) 156, 405
BlsI GCNGC 2 cut(s) 157, 406
Bme1390I CCNGG 1 cut(s) 542
BmiI GGNNCC 1 cut(s) 841
BmrFI CCNGG 1 cut(s) 542
BpiI GAAGAC 2 cut(s) 441, 797
BpmI CTGGAG 1 cut(s) 816
BsaWI WCCGGW 1 cut(s) 433
Bsc4I CCNNNNNNNGG 1 cut(s) 527
Bse1I ACTGG 2 cut(s) 187, 799
BseBI CCWGG 1 cut(s) 542
BseGI GGATG 2 cut(s) 638, 662
BseLI CCNNNNNNNGG 1 cut(s) 527
BseMII CTCAG 3 cut(s) 309, 318, 399
BseNI ACTGG 2 cut(s) 187, 799
BseRI GAGGAG 2 cut(s) 134, 579
BseXI GCAGC 2 cut(s) 167, 391
BsiSI CCGG 1 cut(s) 434
BslI CCNNNNNNNGG 1 cut(s) 527
BsmAI GTCTC 1 cut(s) 20
BsmI GAATGC 1 cut(s) 406
Bsp143I GATC 3 cut(s) 19, 472, 817
BspCNI CTCAG 3 cut(s) 308, 317, 398
BspLI GGNNCC 1 cut(s) 841
BsrI ACTGG 2 cut(s) 187, 799
BssMI GATC 3 cut(s) 19, 472, 817
BssSI CACGAG 1 cut(s) 101
Bst2BI CACGAG 1 cut(s) 101
Bst2UI CCWGG 1 cut(s) 542
Bst4CI ACNGT 1 cut(s) 31
Bst6I CTCTTC 1 cut(s) 597
BstC8I GCNNGC 1 cut(s) 97
BstDEI CTNAG 5 cut(s) 295, 304, 385, 522, 846
BstENI CCTNNNNNAGG 1 cut(s) 525
BstF5I GGATG 2 cut(s) 638, 662
BstKTI GATC 3 cut(s) 22, 475, 820
BstMAI GTCTC 1 cut(s) 20
BstMBI GATC 3 cut(s) 19, 472, 817
BstNI CCWGG 1 cut(s) 542
BstSCI CCNGG 1 cut(s) 540
BstV1I GCAGC 2 cut(s) 167, 391
BstV2I GAAGAC 2 cut(s) 441, 797
BstXI CCANNNNNNTGG 1 cut(s) 436
BtsCI GGATG 2 cut(s) 638, 662
BtsI GCAGTG 1 cut(s) 515
BtsIMutI CAGTG 1 cut(s) 515
Cac8I GCNNGC 1 cut(s) 97
CaiI CAGNNNCTG 1 cut(s) 283
CviAII CATG 2 cut(s) 773, 823
DdeI CTNAG 5 cut(s) 295, 304, 385, 522, 846
DpnI GATC 3 cut(s) 21, 474, 819
DpnII GATC 3 cut(s) 19, 472, 817
Eam1104I CTCTTC 1 cut(s) 597
EarI CTCTTC 1 cut(s) 597
Eco57I CTGAAG 1 cut(s) 554
EcoNI CCTNNNNNAGG 1 cut(s) 525
EcoRI GAATTC 1 cut(s) 299
EcoRII CCWGG 1 cut(s) 540
EcoT22I ATGCAT 1 cut(s) 828
FaeI CATG 2 cut(s) 776, 826
FatI CATG 2 cut(s) 772, 822
FbaI TGATCA 1 cut(s) 19
Fnu4HI GCNGC 2 cut(s) 156, 405
FokI GGATG 2 cut(s) 625, 669
Fsp4HI GCNGC 2 cut(s) 156, 405
FspBI CTAG 2 cut(s) 74, 339
GluI GCNGC 2 cut(s) 156, 405
GsuI CTGGAG 1 cut(s) 816
HapII CCGG 1 cut(s) 434
Hin1II CATG 2 cut(s) 776, 826
HindIII AAGCTT 1 cut(s) 50
HinfI GANTC 6 cut(s) 77, 280, 374, 425, 700, 716
HpaII CCGG 1 cut(s) 434
HphI GGTGA 1 cut(s) 717
Hpy166II GTNNAC 1 cut(s) 7
Hpy188I TCNGA 7 cut(s) 256, 279, 298, 512, 573, 699, 834
Hpy188III TCNNGA 5 cut(s) 23, 148, 164, 387, 413
Hpy8I GTNNAC 1 cut(s) 7
HpyAV CCTTC 3 cut(s) 145, 636, 846
HpyCH4III ACNGT 1 cut(s) 31
HpyCH4V TGCA 6 cut(s) 193, 286, 707, 770, 782, 826
HpyF3I CTNAG 5 cut(s) 295, 304, 385, 522, 846
Hsp92II CATG 2 cut(s) 776, 826
Ksp22I TGATCA 1 cut(s) 19
Kzo9I GATC 3 cut(s) 19, 472, 817
LmnI GCTCC 1 cut(s) 592
Lsp1109I GCAGC 2 cut(s) 167, 391
MaeI CTAG 2 cut(s) 74, 339
MaeIII GTNAC 2 cut(s) 380, 659
MalI GATC 3 cut(s) 21, 474, 819
MboI GATC 3 cut(s) 19, 472, 817
MboII GAAGA 6 cut(s) 441, 543, 584, 686, 741, 802
MluCI AATT 3 cut(s) 64, 299, 808
MlyI GAGTC 1 cut(s) 434
MnlI CCTC 6 cut(s) 112, 304, 600, 621, 633, 853
Mph1103I ATGCAT 1 cut(s) 828
MseI TTAA 1 cut(s) 312
MslI CAYNNNNRTG 1 cut(s) 434
MspA1I CMGCKG 1 cut(s) 227
MspI CCGG 1 cut(s) 434
MspR9I CCNGG 1 cut(s) 542
Mva1269I GAATGC 1 cut(s) 406
MvaI CCWGG 1 cut(s) 542
NdeII GATC 3 cut(s) 19, 472, 817
NlaIII CATG 2 cut(s) 776, 826
NlaIV GGNNCC 1 cut(s) 841
NmuCI GTSAC 1 cut(s) 659
NsiI ATGCAT 1 cut(s) 828
PctI GAATGC 1 cut(s) 406
PfeI GAWTC 5 cut(s) 77, 280, 374, 700, 716
PkrI GCNGC 2 cut(s) 157, 406
PleI GAGTC 1 cut(s) 433
PpsI GAGTC 1 cut(s) 433
Psp6I CCWGG 1 cut(s) 540
PspGI CCWGG 1 cut(s) 540
PspN4I GGNNCC 1 cut(s) 841
PstNI CAGNNNCTG 1 cut(s) 283
PvuII CAGCTG 1 cut(s) 227
RseI CAYNNNNRTG 1 cut(s) 434
SaqAI TTAA 1 cut(s) 312
SatI GCNGC 2 cut(s) 156, 405
Sau3AI GATC 3 cut(s) 19, 472, 817
SchI GAGTC 1 cut(s) 434
ScrFI CCNGG 1 cut(s) 542
SetI ASST 8 cut(s) 42, 54, 160, 201, 229, 619, 725, 845
SmiMI CAYNNNNRTG 1 cut(s) 434
SpeI ACTAGT 1 cut(s) 338
Sse9I AATT 3 cut(s) 64, 299, 808
SspI AATATT 1 cut(s) 499
SspMI CTAG 2 cut(s) 74, 339
StyD4I CCNGG 1 cut(s) 540
TaaI ACNGT 1 cut(s) 31
TaqI TCGA 2 cut(s) 163, 455
TasI AATT 3 cut(s) 64, 299, 808
TfiI GAWTC 5 cut(s) 77, 280, 374, 700, 716
Tru1I TTAA 1 cut(s) 312
Tru9I TTAA 1 cut(s) 312
TscAI CASTG 1 cut(s) 522
TseFI GTSAC 1 cut(s) 659
TseI GCWGC 2 cut(s) 155, 404
Tsp45I GTSAC 1 cut(s) 659
TspRI CASTG 1 cut(s) 522
XagI CCTNNNNNAGG 1 cut(s) 525
XapI RAATTY 1 cut(s) 299
XspI CTAG 2 cut(s) 74, 339
Zsp2I ATGCAT 1 cut(s) 828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.