MD12G1136600.v1.1

Protein of unknown function (DUF1635)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0017139
Physical Location & Seq
Reverse (-)
21320196 .. 21321038
843 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1136600.v1.1.491

Sequence Viewer

Length: 843 bp
ATGGCGAAAGAGGAGGTGAGCAAGAACGAAGAAATCATGAAGCAGTTATTTCAACTCTGGCAGATTACTTGCCAAGAAAGAGATGAAGCAAGAACCCAGTTGCAGAAACTTGTGAATAAGTTAGTGCCATCATCCATACCAGATGATCAAACTTCAATCCTCTCACCACAACTCAATATCAAAAACCCGATCCCCCAAAAAACCCTAGACCCTCCTATGCTCAAGGAATCAGATAGTAATTTTGTGCCCTCCCAATCTTACAACTTTCATGCCCTTGGTTCATATCCCACAAAGAGCAACTTGGGTTCTCCTATCTCCTTCCCAGGCCTACAGCCTAATCCAAACTTTCTGGATTCGGACAACTTGGGGTTTACGAAAAATGTTGTTCCCGTGGGTTCGAAGATCGATTTTGGTTCAGTTCTTATTGACAACATTGCTAGAGTGAGACCACTGCCCAAAAAAGGAAGACTAATGCAGGCTGTGATGGAAGCTGGCCCTATACTACAAACACTTCTTGTTGCACCTCTTCCCAAGTGGAGAAACCCTCCTCCTCTCCCCTCTTTTAAGAATCCGCCAGCGCTCGTTAATGGAGGTGATCATTGTGCCTCATTAGTAGATCAGAAAGAGGAGTTTGATTCGTTTCTTGCGGCGTGGATTTCGTCAAGTGGGTCTGCTTCATTCCTTGAGAGTTCACCTGGATTTTCTCAGATACCATCTTCTTCTGTGTTGAATTTCGCTCGTGGTTCGGCATGTTTGAGCAATGGGATGGAATTGCAATTTGGGAACAACAATGCGGATGCCATGCAGTTTCAGGTTGCAACTGGTAAAAGAAGAAGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1635

Protein Analysis

281

Amino Acids

30.71

Weight (kDa)

8.39

Isoelectric Point (pI)

55.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1635 PF07795 2 - 197 1.1e-24 Protein of unknown function (DUF1635)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017139)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09940
fragaria_vesca FvH4_6g14520
malus_domestica MD12G1136600.v1.1
rosa_chinensis RchiOBHm_Chr3g0467601
rosa_laevigata RLG00000024486
rosa_multiflora Rmu_sc0011632.1_g000011
rosa_roxburghii Rroxscaffold_6G00413560
rosa_rugosa Rorug03G0088400
rosa_samantha Rh3AG136700 Rh3BG158500 Rh3CG158200 Rh3DG159000
rosa_wichuraiana Rw3G012950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 572, 647, 794
AclWI GGATC 1 cut(s) 184
AcsI RAATTY 1 cut(s) 730
AfeI AGCGCT 1 cut(s) 579
AfiI CCNNNNNNNGG 1 cut(s) 461
AgsI TTSAA 3 cut(s) 53, 156, 730
AjnI CCWGG 2 cut(s) 322, 694
AjuI GAANNNNNNNTTGG 2 cut(s) 762, 794
AluBI AGCT 1 cut(s) 491
AluI AGCT 1 cut(s) 491
Alw26I GTCTC 1 cut(s) 439
AlwI GGATC 1 cut(s) 184
Aor51HI AGCGCT 1 cut(s) 579
AoxI GGCC 2 cut(s) 325, 493
ApoI RAATTY 1 cut(s) 730
AspLEI GCGC 1 cut(s) 580
AspS9I GGNCC 1 cut(s) 494
AsuHPI GGTGA 4 cut(s) 28, 156, 605, 684
AsuII TTCGAA 1 cut(s) 398
BaeGI GKGCMC 1 cut(s) 249
BauI CACGAG 1 cut(s) 738
BbsI GAAGAC 1 cut(s) 472
BccI CCATC 4 cut(s) 136, 478, 721, 760
BciT130I CCWGG 2 cut(s) 324, 696
BclI TGATCA 2 cut(s) 145, 595
BcoDI GTCTC 1 cut(s) 439
BfaI CTAG 2 cut(s) 206, 438
BfmI CTRYAG 1 cut(s) 329
BfoI RGCGCY 1 cut(s) 581
BisI GCNGC 1 cut(s) 648
BlsI GCNGC 1 cut(s) 649
Bme1390I CCNGG 2 cut(s) 324, 696
BmgT120I GGNCC 1 cut(s) 494
BmrFI CCNGG 2 cut(s) 324, 696
BmrI ACTGGG 1 cut(s) 91
BmsI GCATC 1 cut(s) 787
BmuI ACTGGG 1 cut(s) 91
BpiI GAAGAC 1 cut(s) 472
BplI GAGNNNNNCTC 2 cut(s) 529, 561
Bpu14I TTCGAA 1 cut(s) 398
BpuEI CTTGAG 2 cut(s) 206, 704
Bsa29I ATCGAT 1 cut(s) 405
BsaI GGTCTC 1 cut(s) 439
BsaJI CCNNGG 3 cut(s) 274, 322, 390
Bsc4I CCNNNNNNNGG 1 cut(s) 461
Bse1I ACTGG 2 cut(s) 97, 826
Bse3DI GCAATG 2 cut(s) 432, 766
BseBI CCWGG 2 cut(s) 324, 696
BseCI ATCGAT 1 cut(s) 405
BseDI CCNNGG 3 cut(s) 274, 322, 390
BseGI GGATG 3 cut(s) 131, 771, 802
BseLI CCNNNNNNNGG 1 cut(s) 461
BseMI GCAATG 2 cut(s) 432, 766
BseMII CTCAG 1 cut(s) 719
BseNI ACTGG 2 cut(s) 97, 826
BseRI GAGGAG 4 cut(s) 26, 537, 540, 641
BseSI GKGCMC 1 cut(s) 249
BshFI GGCC 2 cut(s) 327, 495
BshVI ATCGAT 1 cut(s) 405
BslI CCNNNNNNNGG 1 cut(s) 461
BsmAI GTCTC 1 cut(s) 439
BsnI GGCC 2 cut(s) 327, 495
Bso31I GGTCTC 1 cut(s) 439
Bsp119I TTCGAA 1 cut(s) 398
Bsp1286I GDGCHC 1 cut(s) 249
Bsp143I GATC 5 cut(s) 145, 189, 402, 595, 616
BspACI CCGC 3 cut(s) 572, 647, 794
BspANI GGCC 2 cut(s) 327, 495
BspCNI CTCAG 1 cut(s) 718
BspDI ATCGAT 1 cut(s) 405
BspHI TCATGA 1 cut(s) 36
BspPI GGATC 1 cut(s) 184
BspT104I TTCGAA 1 cut(s) 398
BspTNI GGTCTC 1 cut(s) 439
BsrDI GCAATG 2 cut(s) 432, 766
BsrI ACTGG 2 cut(s) 97, 826
BssECI CCNNGG 3 cut(s) 274, 322, 390
BssMI GATC 5 cut(s) 145, 189, 402, 595, 616
BssSI CACGAG 1 cut(s) 738
BssT1I CCWWGG 1 cut(s) 274
Bst2BI CACGAG 1 cut(s) 738
Bst2UI CCWGG 2 cut(s) 324, 696
Bst6I CTCTTC 1 cut(s) 531
BstBI TTCGAA 1 cut(s) 398
BstC8I GCNNGC 3 cut(s) 477, 493, 576
BstDEI CTNAG 1 cut(s) 705
BstDSI CCRYGG 1 cut(s) 390
BstF5I GGATG 3 cut(s) 131, 771, 802
BstH2I RGCGCY 1 cut(s) 581
BstHHI GCGC 1 cut(s) 580
BstKTI GATC 5 cut(s) 148, 192, 405, 598, 619
BstMAI GTCTC 1 cut(s) 439
BstMBI GATC 5 cut(s) 145, 189, 402, 595, 616
BstNI CCWGG 2 cut(s) 324, 696
BstNSI RCATGY 1 cut(s) 753
BstSCI CCNGG 2 cut(s) 322, 694
BstSFI CTRYAG 1 cut(s) 329
BstSLI GKGCMC 1 cut(s) 249
BstV2I GAAGAC 1 cut(s) 472
Bsu15I ATCGAT 1 cut(s) 405
BsuRI GGCC 2 cut(s) 327, 495
BsuTUI ATCGAT 1 cut(s) 405
BtgI CCRYGG 1 cut(s) 390
BtsCI GGATG 3 cut(s) 131, 771, 802
BtsI GCAGTG 1 cut(s) 449
BtsIMutI CAGTG 1 cut(s) 449
Cac8I GCNNGC 3 cut(s) 477, 493, 576
CciI TCATGA 1 cut(s) 36
CfoI GCGC 1 cut(s) 580
Cfr13I GGNCC 1 cut(s) 494
ClaI ATCGAT 1 cut(s) 405
CviAII CATG 4 cut(s) 37, 269, 750, 802
CviJI RGCY 5 cut(s) 327, 334, 479, 491, 495
CviKI_1 RGCY 5 cut(s) 327, 334, 479, 491, 495
DdeI CTNAG 1 cut(s) 705
DpnI GATC 5 cut(s) 147, 191, 404, 597, 618
DpnII GATC 5 cut(s) 145, 189, 402, 595, 616
Eam1104I CTCTTC 1 cut(s) 531
EarI CTCTTC 1 cut(s) 531
EciI GGCGGA 1 cut(s) 561
Eco130I CCWWGG 1 cut(s) 274
Eco147I AGGCCT 1 cut(s) 327
Eco31I GGTCTC 1 cut(s) 439
Eco47III AGCGCT 1 cut(s) 579
EcoRII CCWGG 2 cut(s) 322, 694
EcoT14I CCWWGG 1 cut(s) 274
ErhI CCWWGG 1 cut(s) 274
FaeI CATG 4 cut(s) 40, 272, 753, 805
FaiI YATR 8 cut(s) 38, 137, 218, 270, 283, 500, 751, 803
FalI AAGNNNNNCTT 2 cut(s) 284, 316
FatI CATG 4 cut(s) 36, 268, 749, 801
FbaI TGATCA 2 cut(s) 145, 595
Fnu4HI GCNGC 1 cut(s) 648
FokI GGATG 3 cut(s) 118, 778, 809
Fsp4HI GCNGC 1 cut(s) 648
FspBI CTAG 2 cut(s) 206, 438
GlaI GCGC 1 cut(s) 579
GluI GCNGC 1 cut(s) 648
HaeII RGCGCY 1 cut(s) 581
HaeIII GGCC 2 cut(s) 327, 495
HhaI GCGC 1 cut(s) 580
Hin1II CATG 4 cut(s) 40, 272, 753, 805
Hin6I GCGC 1 cut(s) 578
HinP1I GCGC 1 cut(s) 578
HinfI GANTC 4 cut(s) 227, 353, 568, 635
HphI GGTGA 4 cut(s) 28, 156, 605, 684
Hpy166II GTNNAC 2 cut(s) 372, 692
Hpy188I TCNGA 4 cut(s) 232, 358, 621, 708
Hpy188III TCNNGA 2 cut(s) 37, 350
Hpy8I GTNNAC 2 cut(s) 372, 692
HpyAV CCTTC 2 cut(s) 328, 828
HpyCH4V TGCA 6 cut(s) 103, 475, 521, 775, 805, 818
HpyF3I CTNAG 1 cut(s) 705
Hsp92II CATG 4 cut(s) 40, 272, 753, 805
HspAI GCGC 1 cut(s) 578
Ksp22I TGATCA 2 cut(s) 145, 595
Kzo9I GATC 5 cut(s) 145, 189, 402, 595, 616
LweI GCATC 1 cut(s) 787
MaeI CTAG 2 cut(s) 206, 438
MalI GATC 5 cut(s) 147, 191, 404, 597, 618
MboI GATC 5 cut(s) 145, 189, 402, 595, 616
MboII GAAGA 7 cut(s) 41, 412, 477, 518, 708, 711, 843
MhlI GDGCHC 1 cut(s) 249
MluCI AATT 4 cut(s) 238, 730, 770, 776
MseI TTAA 2 cut(s) 564, 585
MspR9I CCNGG 2 cut(s) 324, 696
MvaI CCWGG 2 cut(s) 324, 696
NdeII GATC 5 cut(s) 145, 189, 402, 595, 616
NlaIII CATG 4 cut(s) 40, 272, 753, 805
NspI RCATGY 1 cut(s) 753
NspV TTCGAA 1 cut(s) 398
PagI TCATGA 1 cut(s) 36
PceI AGGCCT 1 cut(s) 327
PfeI GAWTC 4 cut(s) 227, 353, 568, 635
PkrI GCNGC 1 cut(s) 649
Psp6I CCWGG 2 cut(s) 322, 694
PspGI CCWGG 2 cut(s) 322, 694
PspPI GGNCC 1 cut(s) 494
SaqAI TTAA 2 cut(s) 564, 585
SatI GCNGC 1 cut(s) 648
Sau3AI GATC 5 cut(s) 145, 189, 402, 595, 616
Sau96I GGNCC 1 cut(s) 494
ScrFI CCNGG 2 cut(s) 324, 696
SduI GDGCHC 1 cut(s) 249
SetI ASST 7 cut(s) 18, 493, 526, 595, 697, 816, 839
SfaNI GCATC 1 cut(s) 787
SfcI CTRYAG 1 cut(s) 329
SfuI TTCGAA 1 cut(s) 398
SmlI CTYRAG 2 cut(s) 221, 683
SmoI CTYRAG 2 cut(s) 221, 683
Sse9I AATT 4 cut(s) 238, 730, 770, 776
SseBI AGGCCT 1 cut(s) 327
SsiI CCGC 3 cut(s) 572, 647, 794
SspMI CTAG 2 cut(s) 206, 438
StuI AGGCCT 1 cut(s) 327
StyD4I CCNGG 2 cut(s) 322, 694
StyI CCWWGG 1 cut(s) 274
TaqI TCGA 2 cut(s) 398, 405
TasI AATT 4 cut(s) 238, 730, 770, 776
TauI GCSGC 1 cut(s) 650
TfiI GAWTC 4 cut(s) 227, 353, 568, 635
Tru1I TTAA 2 cut(s) 564, 585
Tru9I TTAA 2 cut(s) 564, 585
TscAI CASTG 1 cut(s) 456
TspDTI ATGAA 5 cut(s) 53, 99, 257, 270, 666
TspRI CASTG 1 cut(s) 456
XapI RAATTY 1 cut(s) 730
XceI RCATGY 1 cut(s) 753
XspI CTAG 2 cut(s) 206, 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.