Rh3AG136700

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0017139
Physical Location & Seq
Forward (+)
11922099 .. 11923597
1499 bp
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UTR
Exon/CDS
Intron
Rh3AG136700.1

Sequence Viewer

Length: 870 bp
ATGGTTTACATTTGGAGTGATCAAGAGACCATTGATGAGCTGAAATACAAGCTTCTCAGCATCAACTATGAACTAGAATCAGCGCAAATAAGGGCGAAGCAGGAGGTAAGCAAAAACGAGGAGATCACAAAGCAGTTGTTTCATCTCTTGAAGGTAGCTTGTCGAGAAAGAGATGAAGCAAATGACCAGTTGCAAAAACTACTCAAAATGTTCTTGCCCATACCAGCTGAAAATGAAACAAGCCCTTTGTTCTCTGAGGTCAACAATGAAAATTATCCAAGTCTGCAAAAAGCCTCAAATTTCTCAACAGTTAAAGAATCAGATGTTATATCCAAAACTAGTTCATATCCCACTAAATACAACTTTGCTGCTGATTCAAGTAACTTAGGATTTTCCAACAATGCTGCTGTTCAAGGGTGTGATGAGTCCGTTCAGGTGGCTACGATGTCTTCATCAAACTCAAAGATCATTGATCGTGCTGATGTTTTGATTGATAATATTGTTAGGGTCAGATCACTGCCTAAGAAAGGAAGATTGTTCCAGGCTGTAATGGAAAATGAGCCTTTACTTCAGACACTTCTCGTTGCTCCTCTTCCCAAGTGGAAAAACCCTCCACCTCTTCCTCCTTTAAAAAGTTCATCCAATCTCATTTATGGATGTGACTTTGGCTCACAAGAACAGATTGAAGCAGTCAAGCCAATTCGTGCAGTACAAGGTTCAAGCTCACTCGGATTTTCTCACATACCATCTTCATCTGTGTTGGACTTTGAACATGGTTCTGCTAGTGTGAAGACTCGAGTTGGTAACAGTTCAAGTGATCTCATGCAGAGTCAGATTGGGACTTGTAAGAGAAGAAAAAGAAGGTTCTGA

Protein Analysis

289

Amino Acids

32.27

Weight (kDa)

8.23

Isoelectric Point (pI)

52.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1635 PF07795 10 - 207 6.5e-30 Protein of unknown function (DUF1635)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017139)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09940
fragaria_vesca FvH4_6g14520
malus_domestica MD12G1136600.v1.1
rosa_chinensis RchiOBHm_Chr3g0467601
rosa_laevigata RLG00000024486
rosa_multiflora Rmu_sc0011632.1_g000011
rosa_roxburghii Rroxscaffold_6G00413560
rosa_rugosa Rorug03G0088400
rosa_samantha Rh3AG136700 Rh3BG158500 Rh3CG158200 Rh3DG159000
rosa_wichuraiana Rw3G012950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 298
AcuI CTGAAG 1 cut(s) 554
AfaI GTAC 1 cut(s) 711
AfiI CCNNNNNNNGG 1 cut(s) 527
AgsI TTSAA 7 cut(s) 151, 378, 413, 686, 720, 770, 813
AhlI ACTAGT 1 cut(s) 338
AjnI CCWGG 1 cut(s) 540
AluBI AGCT 5 cut(s) 40, 52, 158, 227, 723
AluI AGCT 5 cut(s) 40, 52, 158, 227, 723
Alw26I GTCTC 1 cut(s) 20
Ama87I CYCGRG 1 cut(s) 795
ApeKI GCWGC 2 cut(s) 368, 404
ApoI RAATTY 1 cut(s) 298
AspLEI GCGC 1 cut(s) 85
AvaI CYCGRG 1 cut(s) 795
BbsI GAAGAC 2 cut(s) 441, 797
BbvI GCAGC 2 cut(s) 355, 391
BccI CCATC 1 cut(s) 754
BciT130I CCWGG 1 cut(s) 542
BclI TGATCA 1 cut(s) 19
BcoDI GTCTC 1 cut(s) 20
BcuI ACTAGT 1 cut(s) 338
BfaI CTAG 3 cut(s) 74, 339, 783
BisI GCNGC 2 cut(s) 369, 405
BlsI GCNGC 2 cut(s) 370, 406
Bme1390I CCNGG 1 cut(s) 542
BmeT110I CYCGRG 1 cut(s) 795
BmrFI CCNGG 1 cut(s) 542
BmsI GCATC 1 cut(s) 69
BpiI GAAGAC 2 cut(s) 441, 797
BsaI GGTCTC 1 cut(s) 20
Bsc4I CCNNNNNNNGG 1 cut(s) 527
Bse1I ACTGG 1 cut(s) 187
BseBI CCWGG 1 cut(s) 542
BseGI GGATG 2 cut(s) 638, 662
BseLI CCNNNNNNNGG 1 cut(s) 527
BseMII CTCAG 2 cut(s) 70, 246
BseNI ACTGG 1 cut(s) 187
BseRI GAGGAG 2 cut(s) 134, 579
BseXI GCAGC 2 cut(s) 355, 391
BsgI GTGCAG 1 cut(s) 726
BsiHKCI CYCGRG 1 cut(s) 795
BslFI GGGAC 1 cut(s) 853
BslI CCNNNNNNNGG 1 cut(s) 527
BsmAI GTCTC 1 cut(s) 20
BsmFI GGGAC 1 cut(s) 853
Bso31I GGTCTC 1 cut(s) 20
BsoBI CYCGRG 1 cut(s) 795
Bsp143I GATC 6 cut(s) 19, 123, 465, 472, 512, 817
BspCNI CTCAG 2 cut(s) 69, 247
BspTNI GGTCTC 1 cut(s) 20
BsrI ACTGG 1 cut(s) 187
BssMI GATC 6 cut(s) 19, 123, 465, 472, 512, 817
Bst2UI CCWGG 1 cut(s) 542
Bst4CI ACNGT 2 cut(s) 310, 809
Bst6I CTCTTC 2 cut(s) 597, 624
BstDEI CTNAG 4 cut(s) 56, 255, 385, 522
BstENI CCTNNNNNAGG 1 cut(s) 525
BstF5I GGATG 2 cut(s) 638, 662
BstHHI GCGC 1 cut(s) 85
BstKTI GATC 6 cut(s) 22, 126, 468, 475, 515, 820
BstMAI GTCTC 1 cut(s) 20
BstMBI GATC 6 cut(s) 19, 123, 465, 472, 512, 817
BstNI CCWGG 1 cut(s) 542
BstSCI CCNGG 1 cut(s) 540
BstV1I GCAGC 2 cut(s) 355, 391
BstV2I GAAGAC 2 cut(s) 441, 797
BtsCI GGATG 2 cut(s) 638, 662
BtsI GCAGTG 1 cut(s) 515
BtsIMutI CAGTG 1 cut(s) 515
CfoI GCGC 1 cut(s) 85
Csp6I GTAC 1 cut(s) 710
CviAII CATG 2 cut(s) 773, 823
CviQI GTAC 1 cut(s) 710
DdeI CTNAG 4 cut(s) 56, 255, 385, 522
DpnI GATC 6 cut(s) 21, 125, 467, 474, 514, 819
DpnII GATC 6 cut(s) 19, 123, 465, 472, 512, 817
DraI TTTAAA 1 cut(s) 630
Eam1104I CTCTTC 2 cut(s) 597, 624
EarI CTCTTC 2 cut(s) 597, 624
Eco31I GGTCTC 1 cut(s) 20
Eco57I CTGAAG 1 cut(s) 554
Eco88I CYCGRG 1 cut(s) 795
EcoNI CCTNNNNNAGG 1 cut(s) 525
EcoRII CCWGG 1 cut(s) 540
FaeI CATG 2 cut(s) 776, 826
FaiI YATR 8 cut(s) 69, 221, 329, 346, 654, 743, 774, 824
FaqI GGGAC 1 cut(s) 853
FatI CATG 2 cut(s) 772, 822
FbaI TGATCA 1 cut(s) 19
Fnu4HI GCNGC 2 cut(s) 369, 405
FokI GGATG 2 cut(s) 625, 669
Fsp4HI GCNGC 2 cut(s) 369, 405
FspBI CTAG 3 cut(s) 74, 339, 783
GlaI GCGC 1 cut(s) 84
GluI GCNGC 2 cut(s) 369, 405
HhaI GCGC 1 cut(s) 85
Hin1II CATG 2 cut(s) 776, 826
Hin6I GCGC 1 cut(s) 83
HinP1I GCGC 1 cut(s) 83
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HindIII AAGCTT 1 cut(s) 50
HinfI GANTC 6 cut(s) 77, 317, 374, 425, 793, 829
Hpy166II GTNNAC 2 cut(s) 7, 262
Hpy188I TCNGA 7 cut(s) 256, 322, 512, 573, 731, 834, 869
Hpy188III TCNNGA 3 cut(s) 23, 148, 164
Hpy8I GTNNAC 2 cut(s) 7, 262
HpyAV CCTTC 2 cut(s) 145, 855
HpyCH4III ACNGT 2 cut(s) 310, 809
HpyCH4V TGCA 4 cut(s) 193, 286, 707, 826
HpyF3I CTNAG 4 cut(s) 56, 255, 385, 522
Hsp92II CATG 2 cut(s) 776, 826
HspAI GCGC 1 cut(s) 83
Ksp22I TGATCA 1 cut(s) 19
Kzo9I GATC 6 cut(s) 19, 123, 465, 472, 512, 817
LmnI GCTCC 1 cut(s) 592
LpnPI CCDG 6 cut(s) 86, 200, 237, 419, 527, 554
Lsp1109I GCAGC 2 cut(s) 355, 391
LweI GCATC 1 cut(s) 69
MaeI CTAG 3 cut(s) 74, 339, 783
MaeIII GTNAC 3 cut(s) 380, 659, 803
MalI GATC 6 cut(s) 21, 125, 467, 474, 514, 819
MboI GATC 6 cut(s) 19, 123, 465, 472, 512, 817
MboII GAAGA 7 cut(s) 441, 543, 584, 611, 741, 802, 864
MluCI AATT 3 cut(s) 271, 298, 699
MlyI GAGTC 3 cut(s) 434, 787, 838
MmeI TCCRAC 2 cut(s) 420, 741
MnlI CCTC 8 cut(s) 97, 112, 250, 304, 600, 621, 627, 633
MseI TTAA 2 cut(s) 312, 629
MspA1I CMGCKG 1 cut(s) 227
MspR9I CCNGG 1 cut(s) 542
MvaI CCWGG 1 cut(s) 542
NdeII GATC 6 cut(s) 19, 123, 465, 472, 512, 817
NlaIII CATG 2 cut(s) 776, 826
NmuCI GTSAC 1 cut(s) 659
PaeR7I CTCGAG 1 cut(s) 795
PfeI GAWTC 3 cut(s) 77, 317, 374
PkrI GCNGC 2 cut(s) 370, 406
PleI GAGTC 3 cut(s) 433, 787, 837
PpsI GAGTC 3 cut(s) 433, 787, 837
Psp6I CCWGG 1 cut(s) 540
PspGI CCWGG 1 cut(s) 540
PspXI VCTCGAGB 1 cut(s) 795
PvuII CAGCTG 1 cut(s) 227
RsaI GTAC 1 cut(s) 711
RsaNI GTAC 1 cut(s) 710
SaqAI TTAA 2 cut(s) 312, 629
SatI GCNGC 2 cut(s) 369, 405
Sau3AI GATC 6 cut(s) 19, 123, 465, 472, 512, 817
SchI GAGTC 3 cut(s) 434, 787, 838
ScrFI CCNGG 1 cut(s) 542
SfaNI GCATC 1 cut(s) 69
Sfr274I CTCGAG 1 cut(s) 795
SlaI CTCGAG 1 cut(s) 795
SmlI CTYRAG 1 cut(s) 795
SmoI CTYRAG 1 cut(s) 795
SpeI ACTAGT 1 cut(s) 338
Sse9I AATT 3 cut(s) 271, 298, 699
SspI AATATT 1 cut(s) 499
SspMI CTAG 3 cut(s) 74, 339, 783
StyD4I CCNGG 1 cut(s) 540
TaaI ACNGT 2 cut(s) 310, 809
TaqI TCGA 2 cut(s) 163, 796
TasI AATT 3 cut(s) 271, 298, 699
TatI WGTACW 1 cut(s) 709
TfiI GAWTC 3 cut(s) 77, 317, 374
Tru1I TTAA 2 cut(s) 312, 629
Tru9I TTAA 2 cut(s) 312, 629
TscAI CASTG 1 cut(s) 522
TseFI GTSAC 1 cut(s) 659
TseI GCWGC 2 cut(s) 368, 404
Tsp45I GTSAC 1 cut(s) 659
TspDTI ATGAA 9 cut(s) 84, 131, 189, 249, 282, 333, 441, 627, 741
TspGWI ACGGA 1 cut(s) 418
TspRI CASTG 1 cut(s) 522
XagI CCTNNNNNAGG 1 cut(s) 525
XapI RAATTY 1 cut(s) 298
XhoI CTCGAG 1 cut(s) 795
XspI CTAG 3 cut(s) 74, 339, 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.