Rh3DG159000

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0017139
Physical Location & Seq
Forward (+)
13755775 .. 13757284
1510 bp
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UTR
Exon/CDS
Intron
Rh3DG159000.1

Sequence Viewer

Length: 864 bp
ATGGTTTACATTTGGAGTGATCAAGAGACCATTGATGAGCTGAAATACAAGCTTATCAGCATCAACTATGAACTAGAATCAGCGCAAATAAGGGCGAATCAGGAGGTAAGCAAAAACGAGGAGATCACAAAGCAGTTGTTTCATCTCTTGAAGGTAGCTTGTCGAGAAAGAGATGAAGCAAAAGACCAGTTGCAAAAACTACTCAAAATGTTCTTGCCCATACCAGCTGAAAATGAAACAAGCCCTATGTTCTCTGAGGTCAACAATGAAAATTATCCAAGTTTGCAAAAAGCCTCAAATTTCTCAACAGTTAAAGAATCAGATGTTATATCCAAAACTAGTTCATATCCCACTAAATACAACTTTGCTGCTGATTCAAGTAACTTAGGATTTTCCAAGAATGCTGCTGTTCAAGGGTGTGATGAGTCCGTTCAGGTGGCTACGATGTCTTCATCAAACTCAAAGTTCATTGATCGTGCTGATGTTTTGATTGATAATATTGTTAGGGTCAGACCACTGCCTAAGAAAGGAAGATTGTTCCAGGCTGTAATGGAAAATGAGCCTTTACTTCAGACACTTCTTGTTGCTCCTCTTCCCAAGTGGAAAAACCCTCCACCTCTTCCTCCTTTAAAAAGTTCATCCAATCTCATTTATGACTTTGGCTCACAAGAACAGATTGAAGCAGTCAAGCCAATTCGTGCAGTACAAGGTTCAAGCTCACTCGGATTTTCTCACATACCATCTTCATCTGTGTTGGACTTTGAACATGGTTCTGCTAGTGTGAAGACTCGAGTTGGTAACAGTTCAAGTGATCTCATGCAGAGTCAGATTGGGACTTGTAAGAGAAGAAAAAGAAGGTTCTGA

Protein Analysis

287

Amino Acids

32.18

Weight (kDa)

8.61

Isoelectric Point (pI)

51.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1635 PF07795 10 - 207 6.5e-31 Protein of unknown function (DUF1635)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017139)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09940
fragaria_vesca FvH4_6g14520
malus_domestica MD12G1136600.v1.1
rosa_chinensis RchiOBHm_Chr3g0467601
rosa_laevigata RLG00000024486
rosa_multiflora Rmu_sc0011632.1_g000011
rosa_roxburghii Rroxscaffold_6G00413560
rosa_rugosa Rorug03G0088400
rosa_samantha Rh3AG136700 Rh3BG158500 Rh3CG158200 Rh3DG159000
rosa_wichuraiana Rw3G012950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 298
AcuI CTGAAG 1 cut(s) 554
AfaI GTAC 1 cut(s) 705
AfiI CCNNNNNNNGG 1 cut(s) 527
AgsI TTSAA 7 cut(s) 151, 378, 413, 680, 714, 764, 807
AhlI ACTAGT 1 cut(s) 338
AjnI CCWGG 1 cut(s) 540
AluBI AGCT 5 cut(s) 40, 52, 158, 227, 717
AluI AGCT 5 cut(s) 40, 52, 158, 227, 717
Alw26I GTCTC 1 cut(s) 20
Ama87I CYCGRG 1 cut(s) 789
ApeKI GCWGC 2 cut(s) 368, 404
ApoI RAATTY 1 cut(s) 298
AspLEI GCGC 1 cut(s) 85
AvaI CYCGRG 1 cut(s) 789
BbsI GAAGAC 2 cut(s) 441, 791
BbvI GCAGC 2 cut(s) 355, 391
BccI CCATC 1 cut(s) 748
BciT130I CCWGG 1 cut(s) 542
BclI TGATCA 1 cut(s) 19
BcoDI GTCTC 1 cut(s) 20
BcuI ACTAGT 1 cut(s) 338
BfaI CTAG 3 cut(s) 74, 339, 777
BisI GCNGC 2 cut(s) 369, 405
BlsI GCNGC 2 cut(s) 370, 406
Bme1390I CCNGG 1 cut(s) 542
BmeT110I CYCGRG 1 cut(s) 789
BmrFI CCNGG 1 cut(s) 542
BmsI GCATC 1 cut(s) 69
BpiI GAAGAC 2 cut(s) 441, 791
BsaI GGTCTC 1 cut(s) 20
Bsc4I CCNNNNNNNGG 1 cut(s) 527
Bse1I ACTGG 1 cut(s) 187
BseBI CCWGG 1 cut(s) 542
BseGI GGATG 1 cut(s) 638
BseLI CCNNNNNNNGG 1 cut(s) 527
BseMII CTCAG 1 cut(s) 246
BseNI ACTGG 1 cut(s) 187
BseRI GAGGAG 2 cut(s) 134, 579
BseXI GCAGC 2 cut(s) 355, 391
BsgI GTGCAG 1 cut(s) 720
BsiHKCI CYCGRG 1 cut(s) 789
BslFI GGGAC 1 cut(s) 847
BslI CCNNNNNNNGG 1 cut(s) 527
BsmAI GTCTC 1 cut(s) 20
BsmFI GGGAC 1 cut(s) 847
BsmI GAATGC 1 cut(s) 406
Bso31I GGTCTC 1 cut(s) 20
BsoBI CYCGRG 1 cut(s) 789
Bsp143I GATC 4 cut(s) 19, 123, 472, 811
BspCNI CTCAG 1 cut(s) 247
BspTNI GGTCTC 1 cut(s) 20
BsrI ACTGG 1 cut(s) 187
BssMI GATC 4 cut(s) 19, 123, 472, 811
Bst2UI CCWGG 1 cut(s) 542
Bst4CI ACNGT 2 cut(s) 310, 803
Bst6I CTCTTC 2 cut(s) 597, 624
BstDEI CTNAG 3 cut(s) 255, 385, 522
BstENI CCTNNNNNAGG 1 cut(s) 525
BstF5I GGATG 1 cut(s) 638
BstHHI GCGC 1 cut(s) 85
BstKTI GATC 4 cut(s) 22, 126, 475, 814
BstMAI GTCTC 1 cut(s) 20
BstMBI GATC 4 cut(s) 19, 123, 472, 811
BstNI CCWGG 1 cut(s) 542
BstSCI CCNGG 1 cut(s) 540
BstV1I GCAGC 2 cut(s) 355, 391
BstV2I GAAGAC 2 cut(s) 441, 791
BtsCI GGATG 1 cut(s) 638
BtsI GCAGTG 1 cut(s) 515
BtsIMutI CAGTG 1 cut(s) 515
CfoI GCGC 1 cut(s) 85
Csp6I GTAC 1 cut(s) 704
CviAII CATG 2 cut(s) 767, 817
CviQI GTAC 1 cut(s) 704
DdeI CTNAG 3 cut(s) 255, 385, 522
DpnI GATC 4 cut(s) 21, 125, 474, 813
DpnII GATC 4 cut(s) 19, 123, 472, 811
DraI TTTAAA 1 cut(s) 630
Eam1104I CTCTTC 2 cut(s) 597, 624
EarI CTCTTC 2 cut(s) 597, 624
Eco31I GGTCTC 1 cut(s) 20
Eco57I CTGAAG 1 cut(s) 554
Eco88I CYCGRG 1 cut(s) 789
EcoNI CCTNNNNNAGG 1 cut(s) 525
EcoRII CCWGG 1 cut(s) 540
FaeI CATG 2 cut(s) 770, 820
FaiI YATR 9 cut(s) 69, 221, 248, 329, 346, 654, 737, 768, 818
FaqI GGGAC 1 cut(s) 847
FatI CATG 2 cut(s) 766, 816
FbaI TGATCA 1 cut(s) 19
Fnu4HI GCNGC 2 cut(s) 369, 405
FokI GGATG 1 cut(s) 625
Fsp4HI GCNGC 2 cut(s) 369, 405
FspBI CTAG 3 cut(s) 74, 339, 777
GlaI GCGC 1 cut(s) 84
GluI GCNGC 2 cut(s) 369, 405
HhaI GCGC 1 cut(s) 85
Hin1II CATG 2 cut(s) 770, 820
Hin6I GCGC 1 cut(s) 83
HinP1I GCGC 1 cut(s) 83
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HindIII AAGCTT 1 cut(s) 50
HinfI GANTC 7 cut(s) 77, 97, 317, 374, 425, 787, 823
Hpy166II GTNNAC 2 cut(s) 7, 262
Hpy188I TCNGA 7 cut(s) 256, 322, 512, 573, 725, 828, 863
Hpy188III TCNNGA 4 cut(s) 23, 101, 148, 164
Hpy8I GTNNAC 2 cut(s) 7, 262
HpyAV CCTTC 2 cut(s) 145, 849
HpyCH4III ACNGT 2 cut(s) 310, 803
HpyCH4V TGCA 4 cut(s) 193, 286, 701, 820
HpyF3I CTNAG 3 cut(s) 255, 385, 522
Hsp92II CATG 2 cut(s) 770, 820
HspAI GCGC 1 cut(s) 83
Ksp22I TGATCA 1 cut(s) 19
Kzo9I GATC 4 cut(s) 19, 123, 472, 811
LmnI GCTCC 1 cut(s) 592
LpnPI CCDG 6 cut(s) 86, 200, 237, 419, 527, 554
Lsp1109I GCAGC 2 cut(s) 355, 391
LweI GCATC 1 cut(s) 69
MaeI CTAG 3 cut(s) 74, 339, 777
MaeIII GTNAC 2 cut(s) 380, 797
MalI GATC 4 cut(s) 21, 125, 474, 813
MboI GATC 4 cut(s) 19, 123, 472, 811
MboII GAAGA 7 cut(s) 441, 543, 584, 611, 735, 796, 858
MluCI AATT 3 cut(s) 271, 298, 693
MlyI GAGTC 3 cut(s) 434, 781, 832
MmeI TCCRAC 1 cut(s) 735
MnlI CCTC 8 cut(s) 97, 112, 250, 304, 600, 621, 627, 633
MseI TTAA 2 cut(s) 312, 629
MspA1I CMGCKG 1 cut(s) 227
MspR9I CCNGG 1 cut(s) 542
Mva1269I GAATGC 1 cut(s) 406
MvaI CCWGG 1 cut(s) 542
NdeII GATC 4 cut(s) 19, 123, 472, 811
NlaIII CATG 2 cut(s) 770, 820
PaeR7I CTCGAG 1 cut(s) 789
PctI GAATGC 1 cut(s) 406
PfeI GAWTC 4 cut(s) 77, 97, 317, 374
PkrI GCNGC 2 cut(s) 370, 406
PleI GAGTC 3 cut(s) 433, 781, 831
PpsI GAGTC 3 cut(s) 433, 781, 831
Psp6I CCWGG 1 cut(s) 540
PspGI CCWGG 1 cut(s) 540
PspXI VCTCGAGB 1 cut(s) 789
PvuII CAGCTG 1 cut(s) 227
RsaI GTAC 1 cut(s) 705
RsaNI GTAC 1 cut(s) 704
SaqAI TTAA 2 cut(s) 312, 629
SatI GCNGC 2 cut(s) 369, 405
Sau3AI GATC 4 cut(s) 19, 123, 472, 811
SchI GAGTC 3 cut(s) 434, 781, 832
ScrFI CCNGG 1 cut(s) 542
SfaNI GCATC 1 cut(s) 69
Sfr274I CTCGAG 1 cut(s) 789
SlaI CTCGAG 1 cut(s) 789
SmlI CTYRAG 1 cut(s) 789
SmoI CTYRAG 1 cut(s) 789
SpeI ACTAGT 1 cut(s) 338
Sse9I AATT 3 cut(s) 271, 298, 693
SspI AATATT 1 cut(s) 499
SspMI CTAG 3 cut(s) 74, 339, 777
StyD4I CCNGG 1 cut(s) 540
TaaI ACNGT 2 cut(s) 310, 803
TaqI TCGA 2 cut(s) 163, 790
TasI AATT 3 cut(s) 271, 298, 693
TatI WGTACW 1 cut(s) 703
TfiI GAWTC 4 cut(s) 77, 97, 317, 374
Tru1I TTAA 2 cut(s) 312, 629
Tru9I TTAA 2 cut(s) 312, 629
TscAI CASTG 1 cut(s) 522
TseI GCWGC 2 cut(s) 368, 404
TspGWI ACGGA 1 cut(s) 418
TspRI CASTG 1 cut(s) 522
XagI CCTNNNNNAGG 1 cut(s) 525
XapI RAATTY 1 cut(s) 298
XhoI CTCGAG 1 cut(s) 789
XspI CTAG 3 cut(s) 74, 339, 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.