Rroxscaffold_6G00413560

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0017139
Physical Location & Seq
Reverse (-)
35917151 .. 35918996
1846 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00413560.1

Sequence Viewer

Length: 873 bp
ATGGTTTACATTTGGAGTGATCAAGAGACCATTGATGAGCTGAAATACAAGCTTCTCAGCATCAACTATGAACTAGAATCAGCGCAAATAAGGGCGAATCAGGAGGTAAGCAAAAACGAGGAGATCACAAAGCAGTTGTTTCATCTCTTGAAGGTAGCTTGTCGAGAAAGAGATGAAGCAAAAGACCAGTTGCAAAAACTACTCAAAATGTTCTTGCCCATACCAGCTGAAAATGAAACAAGAAGCCCTATGTTCTCTGAGGTCAACAATGAAAATTATTCAAGTCTGCAAAAAGCCTCAAATTTCTCAACAGTTAAAGAATCAGATGTTATATCCAAAACTAGTTCATATCCCACTAAATACAACTTTGCTGCTGATTCAAGTAACTTAGGATTTTCCAAGAATGATGCTGTTCAAGGGTGTGATGAGTCCGTTCAGGTGGCTACGATGTCTTCATCGAACTCAAAGATCATTGATCGTGCTGATGTTTTGATTGATAATATTGTTAGGGTCAGACCACTGCCTAAGAAAGGAAGATTGTTCCAGGCTGTAATGGAAAATGAGCCTTTACTTCAGACACTTCTTGTTGCTCCTCTTCCCAAGTGGAAAAACCCTCCACCTCTTCCTCCTTTAAAAAGTTCATCCAATCTCATTTATGGATGTGACTTTGGCTCACAAGAACAGATTGAAGCAGTCAAGCCAATTCGTGCAGTACAAGGTTCAAGCTCACTCGGATTTTCTCACATACCATCTTCATCTGTTTTGGACTTTGAACATGGTTCTGCTAGTGTGAAGACTCGAGTTGGTAACAGTTCAAGTGATCTCATGCAGAGTCAGATTGGGACTTGTAAGAGAAGAAAAAGAAGGTTCTGA

Protein Analysis

290

Amino Acids

32.5

Weight (kDa)

8.54

Isoelectric Point (pI)

51.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1635 PF07795 10 - 208 8.8e-31 Protein of unknown function (DUF1635)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017139)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09940
fragaria_vesca FvH4_6g14520
malus_domestica MD12G1136600.v1.1
rosa_chinensis RchiOBHm_Chr3g0467601
rosa_laevigata RLG00000024486
rosa_multiflora Rmu_sc0011632.1_g000011
rosa_roxburghii Rroxscaffold_6G00413560
rosa_rugosa Rorug03G0088400
rosa_samantha Rh3AG136700 Rh3BG158500 Rh3CG158200 Rh3DG159000
rosa_wichuraiana Rw3G012950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 301
AcuI CTGAAG 1 cut(s) 557
AfaI GTAC 1 cut(s) 714
AfiI CCNNNNNNNGG 1 cut(s) 530
AgsI TTSAA 8 cut(s) 151, 282, 381, 416, 689, 723, 773, 816
AhlI ACTAGT 1 cut(s) 341
AjnI CCWGG 1 cut(s) 543
AluBI AGCT 5 cut(s) 40, 52, 158, 227, 726
AluI AGCT 5 cut(s) 40, 52, 158, 227, 726
Alw26I GTCTC 1 cut(s) 20
Ama87I CYCGRG 1 cut(s) 798
ApeKI GCWGC 1 cut(s) 371
ApoI RAATTY 1 cut(s) 301
AspLEI GCGC 1 cut(s) 85
AvaI CYCGRG 1 cut(s) 798
BbsI GAAGAC 2 cut(s) 444, 800
BbvI GCAGC 1 cut(s) 358
BccI CCATC 1 cut(s) 757
BciT130I CCWGG 1 cut(s) 545
BclI TGATCA 1 cut(s) 19
BcoDI GTCTC 1 cut(s) 20
BcuI ACTAGT 1 cut(s) 341
BfaI CTAG 3 cut(s) 74, 342, 786
BisI GCNGC 1 cut(s) 372
BlsI GCNGC 1 cut(s) 373
Bme1390I CCNGG 1 cut(s) 545
BmeT110I CYCGRG 1 cut(s) 798
BmrFI CCNGG 1 cut(s) 545
BmsI GCATC 2 cut(s) 69, 397
BpiI GAAGAC 2 cut(s) 444, 800
BsaI GGTCTC 1 cut(s) 20
Bsc4I CCNNNNNNNGG 1 cut(s) 530
Bse1I ACTGG 1 cut(s) 187
BseBI CCWGG 1 cut(s) 545
BseGI GGATG 2 cut(s) 641, 665
BseLI CCNNNNNNNGG 1 cut(s) 530
BseMII CTCAG 2 cut(s) 70, 249
BseNI ACTGG 1 cut(s) 187
BseRI GAGGAG 2 cut(s) 134, 582
BseXI GCAGC 1 cut(s) 358
BsgI GTGCAG 1 cut(s) 729
BsiHKCI CYCGRG 1 cut(s) 798
BslFI GGGAC 1 cut(s) 856
BslI CCNNNNNNNGG 1 cut(s) 530
BsmAI GTCTC 1 cut(s) 20
BsmFI GGGAC 1 cut(s) 856
Bso31I GGTCTC 1 cut(s) 20
BsoBI CYCGRG 1 cut(s) 798
Bsp143I GATC 5 cut(s) 19, 123, 468, 475, 820
BspCNI CTCAG 2 cut(s) 69, 250
BspTNI GGTCTC 1 cut(s) 20
BsrI ACTGG 1 cut(s) 187
BssMI GATC 5 cut(s) 19, 123, 468, 475, 820
Bst2UI CCWGG 1 cut(s) 545
Bst4CI ACNGT 2 cut(s) 313, 812
Bst6I CTCTTC 2 cut(s) 600, 627
BstDEI CTNAG 4 cut(s) 56, 258, 388, 525
BstENI CCTNNNNNAGG 1 cut(s) 528
BstF5I GGATG 2 cut(s) 641, 665
BstHHI GCGC 1 cut(s) 85
BstKTI GATC 5 cut(s) 22, 126, 471, 478, 823
BstMAI GTCTC 1 cut(s) 20
BstMBI GATC 5 cut(s) 19, 123, 468, 475, 820
BstNI CCWGG 1 cut(s) 545
BstSCI CCNGG 1 cut(s) 543
BstV1I GCAGC 1 cut(s) 358
BstV2I GAAGAC 2 cut(s) 444, 800
BtsCI GGATG 2 cut(s) 641, 665
BtsI GCAGTG 1 cut(s) 518
BtsIMutI CAGTG 1 cut(s) 518
CfoI GCGC 1 cut(s) 85
Csp6I GTAC 1 cut(s) 713
CviAII CATG 2 cut(s) 776, 826
CviQI GTAC 1 cut(s) 713
DdeI CTNAG 4 cut(s) 56, 258, 388, 525
DpnI GATC 5 cut(s) 21, 125, 470, 477, 822
DpnII GATC 5 cut(s) 19, 123, 468, 475, 820
DraI TTTAAA 1 cut(s) 633
Eam1104I CTCTTC 2 cut(s) 600, 627
EarI CTCTTC 2 cut(s) 600, 627
Eco31I GGTCTC 1 cut(s) 20
Eco57I CTGAAG 1 cut(s) 557
Eco88I CYCGRG 1 cut(s) 798
EcoNI CCTNNNNNAGG 1 cut(s) 528
EcoRII CCWGG 1 cut(s) 543
FaeI CATG 2 cut(s) 779, 829
FaiI YATR 9 cut(s) 69, 221, 251, 332, 349, 657, 746, 777, 827
FaqI GGGAC 1 cut(s) 856
FatI CATG 2 cut(s) 775, 825
FbaI TGATCA 1 cut(s) 19
Fnu4HI GCNGC 1 cut(s) 372
FokI GGATG 2 cut(s) 628, 672
Fsp4HI GCNGC 1 cut(s) 372
FspBI CTAG 3 cut(s) 74, 342, 786
GlaI GCGC 1 cut(s) 84
GluI GCNGC 1 cut(s) 372
HhaI GCGC 1 cut(s) 85
Hin1II CATG 2 cut(s) 779, 829
Hin6I GCGC 1 cut(s) 83
HinP1I GCGC 1 cut(s) 83
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HindIII AAGCTT 1 cut(s) 50
HinfI GANTC 7 cut(s) 77, 97, 320, 377, 428, 796, 832
Hpy166II GTNNAC 2 cut(s) 7, 265
Hpy188I TCNGA 7 cut(s) 259, 325, 515, 576, 734, 837, 872
Hpy188III TCNNGA 4 cut(s) 23, 101, 148, 164
Hpy8I GTNNAC 2 cut(s) 7, 265
HpyAV CCTTC 2 cut(s) 145, 858
HpyCH4III ACNGT 2 cut(s) 313, 812
HpyCH4V TGCA 4 cut(s) 193, 289, 710, 829
HpyF3I CTNAG 4 cut(s) 56, 258, 388, 525
Hsp92II CATG 2 cut(s) 779, 829
HspAI GCGC 1 cut(s) 83
Ksp22I TGATCA 1 cut(s) 19
Kzo9I GATC 5 cut(s) 19, 123, 468, 475, 820
LmnI GCTCC 1 cut(s) 595
LpnPI CCDG 6 cut(s) 86, 200, 237, 422, 530, 557
Lsp1109I GCAGC 1 cut(s) 358
LweI GCATC 2 cut(s) 69, 397
MaeI CTAG 3 cut(s) 74, 342, 786
MaeIII GTNAC 3 cut(s) 383, 662, 806
MalI GATC 5 cut(s) 21, 125, 470, 477, 822
MboI GATC 5 cut(s) 19, 123, 468, 475, 820
MboII GAAGA 7 cut(s) 444, 546, 587, 614, 744, 805, 867
MluCI AATT 3 cut(s) 274, 301, 702
MlyI GAGTC 3 cut(s) 437, 790, 841
MnlI CCTC 8 cut(s) 97, 112, 253, 307, 603, 624, 630, 636
MseI TTAA 2 cut(s) 315, 632
MspA1I CMGCKG 1 cut(s) 227
MspR9I CCNGG 1 cut(s) 545
MvaI CCWGG 1 cut(s) 545
NdeII GATC 5 cut(s) 19, 123, 468, 475, 820
NlaIII CATG 2 cut(s) 779, 829
NmuCI GTSAC 1 cut(s) 662
PaeR7I CTCGAG 1 cut(s) 798
PfeI GAWTC 4 cut(s) 77, 97, 320, 377
PkrI GCNGC 1 cut(s) 373
PleI GAGTC 3 cut(s) 436, 790, 840
PpsI GAGTC 3 cut(s) 436, 790, 840
Psp6I CCWGG 1 cut(s) 543
PspGI CCWGG 1 cut(s) 543
PspXI VCTCGAGB 1 cut(s) 798
PvuII CAGCTG 1 cut(s) 227
RsaI GTAC 1 cut(s) 714
RsaNI GTAC 1 cut(s) 713
SaqAI TTAA 2 cut(s) 315, 632
SatI GCNGC 1 cut(s) 372
Sau3AI GATC 5 cut(s) 19, 123, 468, 475, 820
SchI GAGTC 3 cut(s) 437, 790, 841
ScrFI CCNGG 1 cut(s) 545
SfaNI GCATC 2 cut(s) 69, 397
Sfr274I CTCGAG 1 cut(s) 798
SlaI CTCGAG 1 cut(s) 798
SmlI CTYRAG 1 cut(s) 798
SmoI CTYRAG 1 cut(s) 798
SpeI ACTAGT 1 cut(s) 341
Sse9I AATT 3 cut(s) 274, 301, 702
SspI AATATT 1 cut(s) 502
SspMI CTAG 3 cut(s) 74, 342, 786
StyD4I CCNGG 1 cut(s) 543
TaaI ACNGT 2 cut(s) 313, 812
TaqI TCGA 3 cut(s) 163, 458, 799
TasI AATT 3 cut(s) 274, 301, 702
TatI WGTACW 1 cut(s) 712
TfiI GAWTC 4 cut(s) 77, 97, 320, 377
Tru1I TTAA 2 cut(s) 315, 632
Tru9I TTAA 2 cut(s) 315, 632
TscAI CASTG 1 cut(s) 525
TseFI GTSAC 1 cut(s) 662
TseI GCWGC 1 cut(s) 371
Tsp45I GTSAC 1 cut(s) 662
TspDTI ATGAA 9 cut(s) 84, 131, 189, 249, 285, 336, 444, 630, 744
TspGWI ACGGA 1 cut(s) 421
TspRI CASTG 1 cut(s) 525
XagI CCTNNNNNAGG 1 cut(s) 528
XapI RAATTY 1 cut(s) 301
XhoI CTCGAG 1 cut(s) 798
XspI CTAG 3 cut(s) 74, 342, 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.