FvH4_6g16911

expansin-A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0017331
Physical Location & Seq
Reverse (-)
10793412 .. 10794667
1256 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g16911.t1

Sequence Viewer

Length: 795 bp
ATGGGTTTGAGAGCTTTGTTTGGATCATTATTCCTGGTCTTGTTTCTTGTTGTCCATGCTGTCCATGTCAATGCTCATCCACCGAAGTTCCGGGCAACTGAATGGAAACATGCTCATGGAACATTTTATGAAGGAAGCTCCGATACATTTGGTGGAGCTTGTGGATACCATGATGTAGTTAAAGAAGGCTATGGCCTGGAGACAGTAGCATTGAGCAATGCATTGTTCAACAAGGGAGAGATGTGTGGTGCATGCTTCGAACTCAAATGCGAGGACGATCCTCAATGGTGCAAACCCGGGCAACCAAGTCTCATTGTTACAGCAACCAACCAGTGCCCTCCCAACCCCGCGCAGGATAGTAATAACGGAGGATGGTGCAACGAACCCCGTGAACATTTCGACATAGCAAGGCCTGTGTTCGAGACAATTGCCCAGTACAAGGCCGGCATCGTTCCAGTCGCATACCGCAGGATTCCATGTGACCATAAGAAAGGAGGCATCAAATTTACATTATCTGGGAATCCTTATTTCACTGAAGTGTTGGTGTGGAATGTCGCTGGCGCCGGGGATGTGAAAGACGTCAAAGTGAAAACGGAGGAGCATCCCGATCAGTGGCTGCAGATGTCGAGAATGTGGGGACAGAGGTGGGTTTACAACGGAAAATTGTGCGAGTCACAAGGGGCGCTTAGCTTCCAAGTGACATCGGGTGATGGAAGAAGCGTCGATTTGGAATGTGTTGCCCCGAAGGGTTGGGCGTTGGGCAACACTTATGAAGCCAAGAATAATTTCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

265

Amino Acids

29.21

Weight (kDa)

6.14

Isoelectric Point (pI)

31.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXPB1_D1 PF22514 41 - 162 1.2e-09 EXPB1-like domain 1
DPBB_1 PF03330 68 - 155 3.3e-19 Lytic transglycolase
Expansin_C PF01357 167 - 248 1.1e-21 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017331)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g16911
rosa_chinensis RchiOBHm_Chr2g0140121 RchiOBHm_Chr3g0470941
rosa_laevigata RLG00000024231
rosa_multiflora Rmu_sc0005264.1_g000003
rosa_roxburghii Rroxscaffold_6G00410390
rosa_rugosa Rorug03G0114100
rosa_samantha Rh2AG411900 Rh2CG398000 Rh3BG189400 Rh3DG186800
rosa_wichuraiana Rw2G033750 Rw3G015420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 582
AccB1I GGYRCC 1 cut(s) 560
AccII CGCG 1 cut(s) 350
AciI CCGC 2 cut(s) 348, 466
AclWI GGATC 2 cut(s) 31, 272
AcsI RAATTY 1 cut(s) 503
AcuI CTGAAG 1 cut(s) 555
AcyI GRCGYC 2 cut(s) 561, 579
AfaI GTAC 1 cut(s) 437
AfiI CCNNNNNNNGG 3 cut(s) 352, 439, 612
AgsI TTSAA 1 cut(s) 229
AjnI CCWGG 2 cut(s) 33, 195
AjuI GAANNNNNNNTTGG 1 cut(s) 770
AleI CACNNNNGTG 1 cut(s) 536
AluBI AGCT 4 cut(s) 14, 138, 158, 690
AluI AGCT 4 cut(s) 14, 138, 158, 690
Alw26I GTCTC 3 cut(s) 194, 314, 416
AlwI GGATC 2 cut(s) 31, 272
AlwNI CAGNNNCTG 1 cut(s) 616
Ama87I CYCGRG 1 cut(s) 296
AoxI GGCC 3 cut(s) 193, 410, 441
ApeKI GCWGC 1 cut(s) 616
ApoI RAATTY 1 cut(s) 503
Asp700I GAANNNNTTC 1 cut(s) 785
AspLEI GCGC 3 cut(s) 352, 563, 685
AsuC2I CCSGG 4 cut(s) 92, 297, 298, 565
AsuHPI GGTGA 1 cut(s) 719
AsuII TTCGAA 1 cut(s) 258
AvaI CYCGRG 1 cut(s) 296
BaeGI GKGCMC 1 cut(s) 338
BanI GGYRCC 1 cut(s) 560
BarI GAAGNNNNNNTAC 2 cut(s) 127, 159
BbvI GCAGC 1 cut(s) 603
BccI CCATC 2 cut(s) 366, 704
BcgI CGANNNNNNTGC 2 cut(s) 410, 444
BciT130I CCWGG 2 cut(s) 35, 197
BciVI GTATCC 1 cut(s) 158
BcnI CCSGG 4 cut(s) 92, 297, 298, 565
BcoDI GTCTC 3 cut(s) 194, 314, 416
BfmI CTRYAG 1 cut(s) 617
BfoI RGCGCY 2 cut(s) 564, 686
BfuI GTATCC 1 cut(s) 158
BisI GCNGC 1 cut(s) 617
BlpI GCTNAGC 1 cut(s) 686
BlsI GCNGC 1 cut(s) 618
Bme1390I CCNGG 6 cut(s) 35, 92, 197, 297, 298, 565
BmeT110I CYCGRG 1 cut(s) 296
BmiI GGNNCC 1 cut(s) 562
BmrFI CCNGG 6 cut(s) 35, 92, 197, 297, 298, 565
BmrI ACTGGG 1 cut(s) 427
BmsI GCATC 3 cut(s) 456, 507, 610
BmuI ACTGGG 1 cut(s) 427
BpmI CTGGAG 1 cut(s) 218
Bpu1102I GCTNAGC 1 cut(s) 686
Bpu14I TTCGAA 1 cut(s) 258
BpuMI CCSGG 4 cut(s) 92, 297, 298, 565
BsaHI GRCGYC 2 cut(s) 561, 579
BsaJI CCNNGG 2 cut(s) 296, 564
Bsc4I CCNNNNNNNGG 3 cut(s) 352, 439, 612
Bse118I RCCGGY 1 cut(s) 443
Bse1I ACTGG 3 cut(s) 331, 433, 455
Bse3DI GCAATG 1 cut(s) 223
BseBI CCWGG 2 cut(s) 35, 197
BseDI CCNNGG 2 cut(s) 296, 564
BseGI GGATG 4 cut(s) 76, 377, 574, 601
BseLI CCNNNNNNNGG 3 cut(s) 352, 439, 612
BseMI GCAATG 1 cut(s) 223
BseNI ACTGG 3 cut(s) 331, 433, 455
BseRI GAGGAG 1 cut(s) 611
BseSI GKGCMC 1 cut(s) 338
BseXI GCAGC 1 cut(s) 603
Bsh1236I CGCG 1 cut(s) 350
BshFI GGCC 3 cut(s) 195, 412, 443
BshNI GGYRCC 1 cut(s) 560
BsiHKCI CYCGRG 1 cut(s) 296
BsiSI CCGG 4 cut(s) 91, 297, 444, 564
BslFI GGGAC 1 cut(s) 651
BslI CCNNNNNNNGG 3 cut(s) 352, 439, 612
BsmAI GTCTC 3 cut(s) 194, 314, 416
BsmFI GGGAC 1 cut(s) 651
BsnI GGCC 3 cut(s) 195, 412, 443
BsoBI CYCGRG 1 cut(s) 296
Bsp119I TTCGAA 1 cut(s) 258
Bsp1286I GDGCHC 1 cut(s) 338
Bsp143I GATC 3 cut(s) 23, 277, 607
Bsp1720I GCTNAGC 1 cut(s) 686
BspACI CCGC 2 cut(s) 348, 466
BspANI GGCC 3 cut(s) 195, 412, 443
BspFNI CGCG 1 cut(s) 350
BspLI GGNNCC 1 cut(s) 562
BspMAI CTGCAG 1 cut(s) 621
BspPI GGATC 2 cut(s) 31, 272
BspT104I TTCGAA 1 cut(s) 258
BspT107I GGYRCC 1 cut(s) 560
BsrDI GCAATG 1 cut(s) 223
BsrFI RCCGGY 1 cut(s) 443
BsrI ACTGG 3 cut(s) 331, 433, 455
BssAI RCCGGY 1 cut(s) 443
BssECI CCNNGG 2 cut(s) 296, 564
BssMI GATC 3 cut(s) 23, 277, 607
BssNI GRCGYC 2 cut(s) 561, 579
Bst2UI CCWGG 2 cut(s) 35, 197
Bst4CI ACNGT 1 cut(s) 205
BstACI GRCGYC 2 cut(s) 561, 579
BstBI TTCGAA 1 cut(s) 258
BstC8I GCNNGC 3 cut(s) 253, 445, 559
BstDEI CTNAG 1 cut(s) 686
BstF5I GGATG 4 cut(s) 76, 377, 574, 601
BstFNI CGCG 1 cut(s) 350
BstH2I RGCGCY 2 cut(s) 564, 686
BstHHI GCGC 3 cut(s) 352, 563, 685
BstKTI GATC 3 cut(s) 26, 280, 610
BstMAI GTCTC 3 cut(s) 194, 314, 416
BstMBI GATC 3 cut(s) 23, 277, 607
BstNI CCWGG 2 cut(s) 35, 197
BstNSI RCATGY 2 cut(s) 113, 255
BstSCI CCNGG 6 cut(s) 33, 90, 195, 295, 296, 563
BstSFI CTRYAG 1 cut(s) 617
BstSLI GKGCMC 1 cut(s) 338
BstUI CGCG 1 cut(s) 350
BstV1I GCAGC 1 cut(s) 603
BsuI GTATCC 1 cut(s) 158
BsuRI GGCC 3 cut(s) 195, 412, 443
BtsCI GGATG 4 cut(s) 76, 377, 574, 601
BtsIMutI CAGTG 3 cut(s) 338, 531, 617
Cac8I GCNNGC 3 cut(s) 253, 445, 559
CaiI CAGNNNCTG 1 cut(s) 616
CfoI GCGC 3 cut(s) 352, 563, 685
Cfr10I RCCGGY 1 cut(s) 443
Cfr9I CCCGGG 1 cut(s) 296
CseI GACGC 1 cut(s) 709
Csp6I GTAC 1 cut(s) 436
CviAII CATG 7 cut(s) 56, 65, 110, 116, 170, 252, 477
CviQI GTAC 1 cut(s) 436
DdeI CTNAG 1 cut(s) 686
DinI GGCGCC 1 cut(s) 562
DpnI GATC 3 cut(s) 25, 279, 609
DpnII GATC 3 cut(s) 23, 277, 607
Eco147I AGGCCT 1 cut(s) 412
Eco57I CTGAAG 1 cut(s) 555
Eco88I CYCGRG 1 cut(s) 296
EcoRII CCWGG 2 cut(s) 33, 195
EcoT22I ATGCAT 1 cut(s) 223
EgeI GGCGCC 1 cut(s) 562
EheI GGCGCC 1 cut(s) 562
FaeI CATG 7 cut(s) 59, 68, 113, 119, 173, 255, 480
FalI AAGNNNNNCTT 2 cut(s) 669, 701
FaqI GGGAC 1 cut(s) 651
FatI CATG 7 cut(s) 55, 64, 109, 115, 169, 251, 476
FauI CCCGC 1 cut(s) 355
Fnu4HI GCNGC 1 cut(s) 617
FokI GGATG 4 cut(s) 63, 384, 581, 588
Fsp4HI GCNGC 1 cut(s) 617
GlaI GCGC 3 cut(s) 351, 562, 684
GluI GCNGC 1 cut(s) 617
GsuI CTGGAG 1 cut(s) 218
HaeII RGCGCY 2 cut(s) 564, 686
HaeIII GGCC 3 cut(s) 195, 412, 443
HapII CCGG 4 cut(s) 91, 297, 444, 564
HgaI GACGC 1 cut(s) 709
HhaI GCGC 3 cut(s) 352, 563, 685
Hin1I GRCGYC 2 cut(s) 561, 579
Hin1II CATG 7 cut(s) 59, 68, 113, 119, 173, 255, 480
Hin6I GCGC 3 cut(s) 350, 561, 683
HinP1I GCGC 3 cut(s) 350, 561, 683
HinfI GANTC 3 cut(s) 472, 520, 671
HpaII CCGG 4 cut(s) 91, 297, 444, 564
HphI GGTGA 1 cut(s) 719
Hpy166II GTNNAC 2 cut(s) 392, 652
Hpy188I TCNGA 1 cut(s) 142
Hpy188III TCNNGA 3 cut(s) 421, 605, 627
Hpy8I GTNNAC 2 cut(s) 392, 652
Hpy99I CGWCG 1 cut(s) 725
HpyAV CCTTC 3 cut(s) 125, 179, 739
HpyCH4III ACNGT 1 cut(s) 205
HpyCH4IV ACGT 1 cut(s) 579
HpyCH4V TGCA 5 cut(s) 221, 251, 291, 378, 619
HpyF3I CTNAG 1 cut(s) 686
HpySE526I ACGT 1 cut(s) 579
Hsp92I GRCGYC 2 cut(s) 561, 579
Hsp92II CATG 7 cut(s) 59, 68, 113, 119, 173, 255, 480
HspAI GCGC 3 cut(s) 350, 561, 683
KasI GGCGCC 1 cut(s) 560
KroI GCCGGC 1 cut(s) 443
KroNI GCCGGC 1 cut(s) 445
Kzo9I GATC 3 cut(s) 23, 277, 607
LmnI GCTCC 3 cut(s) 143, 155, 598
Lsp1109I GCAGC 1 cut(s) 603
LweI GCATC 3 cut(s) 456, 507, 610
MaeII ACGT 1 cut(s) 579
MaeIII GTNAC 4 cut(s) 316, 479, 672, 697
MalI GATC 3 cut(s) 25, 279, 609
MboI GATC 3 cut(s) 23, 277, 607
MboII GAAGA 1 cut(s) 726
MfeI CAATTG 1 cut(s) 426
MhlI GDGCHC 1 cut(s) 338
MluCI AATT 4 cut(s) 426, 503, 662, 784
Mly113I GGCGCC 1 cut(s) 561
MlyI GAGTC 1 cut(s) 680
MnlI CCTC 7 cut(s) 265, 291, 348, 362, 488, 589, 636
Mph1103I ATGCAT 1 cut(s) 223
MroNI GCCGGC 1 cut(s) 443
MroXI GAANNNNTTC 1 cut(s) 785
MseI TTAA 1 cut(s) 180
MslI CAYNNNNRTG 3 cut(s) 69, 114, 536
MspI CCGG 4 cut(s) 91, 297, 444, 564
MspR9I CCNGG 6 cut(s) 35, 92, 197, 297, 298, 565
MunI CAATTG 1 cut(s) 426
MvaI CCWGG 2 cut(s) 35, 197
MvnI CGCG 1 cut(s) 350
NaeI GCCGGC 1 cut(s) 445
NarI GGCGCC 1 cut(s) 561
NciI CCSGG 4 cut(s) 92, 297, 298, 565
NdeII GATC 3 cut(s) 23, 277, 607
NgoMIV GCCGGC 1 cut(s) 443
NlaIII CATG 7 cut(s) 59, 68, 113, 119, 173, 255, 480
NlaIV GGNNCC 1 cut(s) 562
NmuCI GTSAC 3 cut(s) 479, 672, 697
NsiI ATGCAT 1 cut(s) 223
NspI RCATGY 2 cut(s) 113, 255
NspV TTCGAA 1 cut(s) 258
OliI CACNNNNGTG 1 cut(s) 536
PaeI GCATGC 1 cut(s) 255
PceI AGGCCT 1 cut(s) 412
PdiI GCCGGC 1 cut(s) 445
PdmI GAANNNNTTC 1 cut(s) 785
PfeI GAWTC 2 cut(s) 472, 520
PkrI GCNGC 1 cut(s) 618
PleI GAGTC 1 cut(s) 679
PluTI GGCGCC 1 cut(s) 564
PpsI GAGTC 1 cut(s) 679
Psp6I CCWGG 2 cut(s) 33, 195
PspGI CCWGG 2 cut(s) 33, 195
PspN4I GGNNCC 1 cut(s) 562
PstI CTGCAG 1 cut(s) 621
PstNI CAGNNNCTG 1 cut(s) 616
RsaI GTAC 1 cut(s) 437
RsaNI GTAC 1 cut(s) 436
RseI CAYNNNNRTG 3 cut(s) 69, 114, 536
SaqAI TTAA 1 cut(s) 180
SatI GCNGC 1 cut(s) 617
Sau3AI GATC 3 cut(s) 23, 277, 607
SchI GAGTC 1 cut(s) 680
ScrFI CCNGG 6 cut(s) 35, 92, 197, 297, 298, 565
SduI GDGCHC 1 cut(s) 338
SetI ASST 6 cut(s) 16, 140, 160, 582, 647, 692
SfaNI GCATC 3 cut(s) 456, 507, 610
SfcI CTRYAG 1 cut(s) 617
SfoI GGCGCC 1 cut(s) 562
SfuI TTCGAA 1 cut(s) 258
SmaI CCCGGG 1 cut(s) 298
SmiMI CAYNNNNRTG 3 cut(s) 69, 114, 536
SphI GCATGC 1 cut(s) 255
Sse9I AATT 4 cut(s) 426, 503, 662, 784
SseBI AGGCCT 1 cut(s) 412
SsiI CCGC 2 cut(s) 348, 466
SspDI GGCGCC 1 cut(s) 560
StuI AGGCCT 1 cut(s) 412
StyD4I CCNGG 6 cut(s) 33, 90, 195, 295, 296, 563
TaaI ACNGT 1 cut(s) 205
TaiI ACGT 1 cut(s) 582
TaqI TCGA 6 cut(s) 258, 399, 420, 626, 723, 789
TasI AATT 4 cut(s) 426, 503, 662, 784
TatI WGTACW 1 cut(s) 435
TfiI GAWTC 2 cut(s) 472, 520
Tru1I TTAA 1 cut(s) 180
Tru9I TTAA 1 cut(s) 180
TscAI CASTG 3 cut(s) 338, 538, 617
TseFI GTSAC 3 cut(s) 479, 672, 697
TseI GCWGC 1 cut(s) 616
Tsp45I GTSAC 3 cut(s) 479, 672, 697
TspDTI ATGAA 2 cut(s) 144, 786
TspGWI ACGGA 3 cut(s) 381, 608, 672
TspMI CCCGGG 1 cut(s) 296
TspRI CASTG 3 cut(s) 338, 538, 617
XapI RAATTY 1 cut(s) 503
XceI RCATGY 2 cut(s) 113, 255
XmaI CCCGGG 1 cut(s) 296
XmnI GAANNNNTTC 1 cut(s) 785
ZraI GACGTC 1 cut(s) 580
Zsp2I ATGCAT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.