Rorug03G0114100

expansin-A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0017331
Physical Location & Seq
Reverse (-)
9265085 .. 9267939
2855 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0114100.1

Sequence Viewer

Length: 660 bp
ATGTGGGTTGGAGTACTTGACTTATGGGTCTTTGTTATTCAGGGAAATGCTTCCTCTGGCATAGGTGTTGCTGAGGACTGCCGCAACACATTCTTGGAACTGCAGAGAAAGAAGGCATTTCGCTATGTGATTTTTAAGATCGATGAGAAGAAGAAAGAGGTTGTGGTAGAAAAGACTGGAGGCCCAGCTGAAAGTTATGATGATTTCACTGAAAAGATAAGCCAGAGGATGAAAATTCTTCAGGATTTGGTTCCTGGATGTAATAAGGTTATTGGGAAAACACTTGTTCTTGATGAGATTATTAATTACATCCAGTCACTGCAGCGCCAAGTCGAGTTCCTTTCGTTGAACCTTGAAGCAGTCAATTCAAGGGTGAACATGAATCCCGCCATTGAAGCGTTTCCTTCAAAAGATCAATTTGATGCAGCTGCGTTGTTATTTGGACCACACACACCAAGGGAGTATGTGCAGGGCTCGAAAGCCGAATGGCTGCATATGCAGACAACAGATAGCATAGTAGTCGCTGTTGTTGTAAACTTTATCAAACGACAGTTTTTTGGGTTTTTGTTACCTGGCAAGGTTTTTAACGAGGCAACGGATGAAGCGTCACCACCAAGTTTGAGCGGCACAAGGGGGAGTGTTGAAGGAAAATCGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

219

Amino Acids

24.46

Weight (kDa)

5.81

Isoelectric Point (pI)

33.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 20 - 75 2.5e-09 Cofilin/tropomyosin-type actin-binding protein
bHLH_SAC51 PF23173 71 - 111 3.8e-06 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017331)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g16911
rosa_chinensis RchiOBHm_Chr2g0140121 RchiOBHm_Chr3g0470941
rosa_laevigata RLG00000024231
rosa_multiflora Rmu_sc0005264.1_g000003
rosa_roxburghii Rroxscaffold_6G00410390
rosa_rugosa Rorug03G0114100
rosa_samantha Rh2AG411900 Rh2CG398000 Rh3BG189400 Rh3DG186800
rosa_wichuraiana Rw2G033750 Rw3G015420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 26
AccBSI CCGCTC 1 cut(s) 624
AciI CCGC 3 cut(s) 82, 387, 624
AcsI RAATTY 1 cut(s) 234
AcuI CTGAAG 1 cut(s) 224
AfaI GTAC 1 cut(s) 15
AgsI TTSAA 6 cut(s) 349, 356, 369, 395, 408, 644
AjnI CCWGG 2 cut(s) 253, 571
AluBI AGCT 2 cut(s) 188, 428
AluI AGCT 2 cut(s) 188, 428
AlwNI CAGNNNCTG 1 cut(s) 319
AoxI GGCC 1 cut(s) 181
ApeKI GCWGC 4 cut(s) 322, 425, 428, 490
ApoI RAATTY 1 cut(s) 234
AseI ATTAAT 1 cut(s) 303
Asp700I GAANNNNTTC 2 cut(s) 49, 399
AspLEI GCGC 1 cut(s) 327
AspS9I GGNCC 2 cut(s) 182, 443
AsuHPI GGTGA 2 cut(s) 385, 600
AvaII GGWCC 1 cut(s) 443
BanII GRGCYC 1 cut(s) 476
BbvCI CCTCAGC 1 cut(s) 72
BbvI GCAGC 4 cut(s) 334, 415, 437, 477
BcgI CGANNNNNNTGC 2 cut(s) 502, 536
BciT130I CCWGG 2 cut(s) 255, 573
BfmI CTRYAG 2 cut(s) 101, 320
BfoI RGCGCY 1 cut(s) 328
BisI GCNGC 6 cut(s) 82, 323, 426, 429, 491, 625
BlsI GCNGC 6 cut(s) 83, 324, 427, 430, 492, 626
BmcAI AGTACT 1 cut(s) 15
Bme1390I CCNGG 2 cut(s) 255, 573
Bme18I GGWCC 1 cut(s) 443
BmgT120I GGNCC 2 cut(s) 182, 443
BmiI GGNNCC 1 cut(s) 252
BmrFI CCNGG 2 cut(s) 255, 573
BmsI GCATC 1 cut(s) 412
BpmI CTGGAG 1 cut(s) 198
Bpu10I CCTNAGC 1 cut(s) 72
Bsa29I ATCGAT 1 cut(s) 141
BsaJI CCNNGG 1 cut(s) 455
Bse1I ACTGG 2 cut(s) 181, 313
BseBI CCWGG 2 cut(s) 255, 573
BseCI ATCGAT 1 cut(s) 141
BseDI CCNNGG 1 cut(s) 455
BseGI GGATG 4 cut(s) 234, 263, 309, 604
BseMII CTCAG 1 cut(s) 63
BseNI ACTGG 2 cut(s) 181, 313
BseXI GCAGC 4 cut(s) 334, 415, 437, 477
BseYI CCCAGC 1 cut(s) 184
BsgI GTGCAG 1 cut(s) 488
BshFI GGCC 1 cut(s) 183
BshVI ATCGAT 1 cut(s) 141
BsnI GGCC 1 cut(s) 183
Bsp1286I GDGCHC 1 cut(s) 476
Bsp143I GATC 2 cut(s) 138, 412
BspACI CCGC 3 cut(s) 82, 387, 624
BspANI GGCC 1 cut(s) 183
BspCNI CTCAG 1 cut(s) 64
BspDI ATCGAT 1 cut(s) 141
BspLI GGNNCC 1 cut(s) 252
BspMAI CTGCAG 2 cut(s) 105, 324
BsrBI CCGCTC 1 cut(s) 624
BsrI ACTGG 2 cut(s) 181, 313
BssECI CCNNGG 1 cut(s) 455
BssMI GATC 2 cut(s) 138, 412
BssT1I CCWWGG 1 cut(s) 455
Bst2UI CCWGG 2 cut(s) 255, 573
Bst4CI ACNGT 1 cut(s) 552
BstDEI CTNAG 2 cut(s) 72, 657
BstF5I GGATG 4 cut(s) 234, 263, 309, 604
BstH2I RGCGCY 1 cut(s) 328
BstHHI GCGC 1 cut(s) 327
BstKTI GATC 2 cut(s) 141, 415
BstMBI GATC 2 cut(s) 138, 412
BstMWI GCNNNNNNNGC 2 cut(s) 395, 496
BstNI CCWGG 2 cut(s) 255, 573
BstSCI CCNGG 2 cut(s) 253, 571
BstSFI CTRYAG 2 cut(s) 101, 320
BstV1I GCAGC 4 cut(s) 334, 415, 437, 477
Bsu15I ATCGAT 1 cut(s) 141
BsuRI GGCC 1 cut(s) 183
BsuTUI ATCGAT 1 cut(s) 141
BtsCI GGATG 4 cut(s) 234, 263, 309, 604
BtsI GCAGTG 1 cut(s) 317
BtsIMutI CAGTG 2 cut(s) 207, 317
CaiI CAGNNNCTG 1 cut(s) 319
CfoI GCGC 1 cut(s) 327
Cfr13I GGNCC 2 cut(s) 182, 443
ClaI ATCGAT 1 cut(s) 141
CseI GACGC 1 cut(s) 594
Csp6I GTAC 1 cut(s) 14
CspCI CAANNNNNGTGG 2 cut(s) 600, 635
CviAII CATG 1 cut(s) 379
CviJI RGCY 7 cut(s) 183, 188, 222, 428, 474, 482, 490
CviKI_1 RGCY 7 cut(s) 183, 188, 222, 428, 474, 482, 490
CviQI GTAC 1 cut(s) 14
DdeI CTNAG 2 cut(s) 72, 657
DpnI GATC 2 cut(s) 140, 414
DpnII GATC 2 cut(s) 138, 412
DrdI GACNNNNNNGTC 1 cut(s) 26
DseDI GACNNNNNNGTC 1 cut(s) 26
Eco130I CCWWGG 1 cut(s) 455
Eco24I GRGCYC 1 cut(s) 476
Eco47I GGWCC 1 cut(s) 443
Eco57I CTGAAG 1 cut(s) 224
EcoRII CCWGG 2 cut(s) 253, 571
EcoT14I CCWWGG 1 cut(s) 455
EcoT38I GRGCYC 1 cut(s) 476
ErhI CCWWGG 1 cut(s) 455
FaeI CATG 1 cut(s) 382
FaiI YATR 9 cut(s) 25, 62, 126, 198, 380, 465, 495, 497, 515
FatI CATG 1 cut(s) 378
FauI CCCGC 1 cut(s) 394
FauNDI CATATG 1 cut(s) 495
Fnu4HI GCNGC 6 cut(s) 82, 323, 426, 429, 491, 625
FokI GGATG 4 cut(s) 241, 270, 296, 611
FriOI GRGCYC 1 cut(s) 476
Fsp4HI GCNGC 6 cut(s) 82, 323, 426, 429, 491, 625
GlaI GCGC 1 cut(s) 326
GluI GCNGC 6 cut(s) 82, 323, 426, 429, 491, 625
GsaI CCCAGC 1 cut(s) 188
GsuI CTGGAG 1 cut(s) 198
HaeII RGCGCY 1 cut(s) 328
HaeIII GGCC 1 cut(s) 183
HgaI GACGC 1 cut(s) 594
HhaI GCGC 1 cut(s) 327
Hin1II CATG 1 cut(s) 382
Hin6I GCGC 1 cut(s) 325
HinP1I GCGC 1 cut(s) 325
HinfI GANTC 1 cut(s) 382
HphI GGTGA 2 cut(s) 385, 600
Hpy166II GTNNAC 2 cut(s) 376, 535
Hpy188III TCNNGA 2 cut(s) 242, 290
Hpy8I GTNNAC 2 cut(s) 376, 535
HpyAV CCTTC 3 cut(s) 106, 414, 638
HpyCH4III ACNGT 1 cut(s) 552
HpyCH4V TGCA 6 cut(s) 103, 322, 425, 469, 493, 499
HpyF10VI GCNNNNNNNGC 2 cut(s) 395, 496
HpyF3I CTNAG 2 cut(s) 72, 657
Hsp92II CATG 1 cut(s) 382
HspAI GCGC 1 cut(s) 325
Kzo9I GATC 2 cut(s) 138, 412
Lsp1109I GCAGC 4 cut(s) 334, 415, 437, 477
LweI GCATC 1 cut(s) 412
MaeIII GTNAC 3 cut(s) 315, 567, 606
MalI GATC 2 cut(s) 140, 414
MbiI CCGCTC 1 cut(s) 624
MboI GATC 2 cut(s) 138, 412
MboII GAAGA 3 cut(s) 160, 163, 230
MhlI GDGCHC 1 cut(s) 476
MluCI AATT 4 cut(s) 234, 304, 364, 416
MnlI CCTC 6 cut(s) 64, 67, 151, 173, 219, 583
MroXI GAANNNNTTC 2 cut(s) 49, 399
MseI TTAA 3 cut(s) 135, 303, 585
MspA1I CMGCKG 2 cut(s) 188, 428
MspR9I CCNGG 2 cut(s) 255, 573
MvaI CCWGG 2 cut(s) 255, 573
MwoI GCNNNNNNNGC 2 cut(s) 395, 496
NdeI CATATG 1 cut(s) 495
NdeII GATC 2 cut(s) 138, 412
NlaIII CATG 1 cut(s) 382
NlaIV GGNNCC 1 cut(s) 252
NmuCI GTSAC 2 cut(s) 315, 606
PcsI WCGNNNNNNNCGW 1 cut(s) 602
PdmI GAANNNNTTC 2 cut(s) 49, 399
PfeI GAWTC 1 cut(s) 382
PfoI TCCNGGA 1 cut(s) 253
PkrI GCNGC 6 cut(s) 83, 324, 427, 430, 492, 626
PshBI ATTAAT 1 cut(s) 303
Psp6I CCWGG 2 cut(s) 253, 571
PspFI CCCAGC 1 cut(s) 184
PspGI CCWGG 2 cut(s) 253, 571
PspN4I GGNNCC 1 cut(s) 252
PspPI GGNCC 2 cut(s) 182, 443
PstI CTGCAG 2 cut(s) 105, 324
PstNI CAGNNNCTG 1 cut(s) 319
PvuII CAGCTG 2 cut(s) 188, 428
RsaI GTAC 1 cut(s) 15
RsaNI GTAC 1 cut(s) 14
SaqAI TTAA 3 cut(s) 135, 303, 585
SatI GCNGC 6 cut(s) 82, 323, 426, 429, 491, 625
Sau3AI GATC 2 cut(s) 138, 412
Sau96I GGNCC 2 cut(s) 182, 443
ScaI AGTACT 1 cut(s) 15
ScrFI CCNGG 2 cut(s) 255, 573
SduI GDGCHC 1 cut(s) 476
SetI ASST 8 cut(s) 67, 162, 190, 270, 354, 430, 574, 582
SfaNI GCATC 1 cut(s) 412
SfcI CTRYAG 2 cut(s) 101, 320
SinI GGWCC 1 cut(s) 443
Sse9I AATT 4 cut(s) 234, 304, 364, 416
SsiI CCGC 3 cut(s) 82, 387, 624
StyD4I CCNGG 2 cut(s) 253, 571
StyI CCWWGG 1 cut(s) 455
TaaI ACNGT 1 cut(s) 552
TaqI TCGA 4 cut(s) 141, 333, 476, 653
TasI AATT 4 cut(s) 234, 304, 364, 416
TatI WGTACW 1 cut(s) 13
TauI GCSGC 2 cut(s) 84, 627
TfiI GAWTC 1 cut(s) 382
Tru1I TTAA 3 cut(s) 135, 303, 585
Tru9I TTAA 3 cut(s) 135, 303, 585
TscAI CASTG 2 cut(s) 214, 324
TseFI GTSAC 2 cut(s) 315, 606
TseI GCWGC 4 cut(s) 322, 425, 428, 490
Tsp45I GTSAC 2 cut(s) 315, 606
TspDTI ATGAA 3 cut(s) 245, 395, 615
TspGWI ACGGA 1 cut(s) 611
TspRI CASTG 2 cut(s) 214, 324
VpaK11BI GGWCC 1 cut(s) 443
VspI ATTAAT 1 cut(s) 303
XapI RAATTY 1 cut(s) 234
XmnI GAANNNNTTC 2 cut(s) 49, 399
ZrmI AGTACT 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.