Rw2G033750

expansin-A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0017331
Physical Location & Seq
Forward (+)
55128450 .. 55131331
2882 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G033750.1

Sequence Viewer

Length: 798 bp
ATGTCTTTGAGAGCTTTGTGTTGGTCGTTCCTTGTCTTCTTTTTTGTTGTCTATGTCAATGCTCATGATCATCATAATCCAAAGCCTCATGACCAGCAGAAGCCAAGGCCTGATGATCAGCAGAAGCCAAAGTTGCAGTCAACTGAATGGAAACATGCTCATGCCACATTCTATGAAGGAGGTTCCGGAACATTTGGTAAGAACTTATGGTCGACAACCACCCTTTATTTTACAAGTACCCGCGCATTGAGCAATGCATTGTTCAACAAGGGGGAGATGTGTGGTGCATGCTTCGAAATCAAATGCGAGGACTCGCCTCAATGGTGCAAGCCTGGCCAGCCAAGTCTCATTGTTACAGCGACCAACCAGTGCCCTCCTAATTACAACCAACCGAGTGATAACGGAGGATGGTGCAACGAACCCCGTGAACATTTCGACATAGCAAGGCCTGTGTTCGAGACTATTGCTGCGTACAAGGCCGGCATCGTTCCAGTCTCATACCGCAGGATTCCATGTGACCACAAGACTGGAGGCATTAAATTTACAATAACTGGGAATCCTTATTTCAATGAAGTGTTGGTGTGGAATGTTGGAGGTGCTGGGTATGTATGCAACGTTGAAGTGAAACCTGAAGACCAACCGAATACATGGCTGCCGTTGCAGCGAATGTGGGGTCAGCGGTGGATTGACAAAGGAAAATTTGACATCGGTGATGGAAGAACCGTAATCTCTGAAAATGTTCTCCCAAAGGGATGGCAGTTTGGCCAGACTTATGAAGGCAAGAATTTCGCCGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

265

Amino Acids

30.07

Weight (kDa)

8.03

Isoelectric Point (pI)

36.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 81 - 167 4e-19 Lytic transglycolase
EXPB1_D1 PF22514 81 - 174 5.9e-07 EXPB1-like domain 1
Expansin_C PF01357 179 - 249 9.1e-14 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017331)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g16911
rosa_chinensis RchiOBHm_Chr2g0140121 RchiOBHm_Chr3g0470941
rosa_laevigata RLG00000024231
rosa_multiflora Rmu_sc0005264.1_g000003
rosa_roxburghii Rroxscaffold_6G00410390
rosa_rugosa Rorug03G0114100
rosa_samantha Rh2AG411900 Rh2CG398000 Rh3BG189400 Rh3DG186800
rosa_wichuraiana Rw2G033750 Rw3G015420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 212
AccII CGCG 1 cut(s) 243
AccIII TCCGGA 1 cut(s) 185
AciI CCGC 3 cut(s) 241, 502, 679
AclI AACGTT 1 cut(s) 615
AcoI YGGCCR 2 cut(s) 334, 763
AcsI RAATTY 3 cut(s) 539, 698, 784
AcuI CTGAAG 1 cut(s) 651
AfaI GTAC 2 cut(s) 238, 473
AgsI TTSAA 3 cut(s) 265, 568, 620
AjnI CCWGG 1 cut(s) 331
AluBI AGCT 1 cut(s) 14
AluI AGCT 1 cut(s) 14
Alw26I GTCTC 3 cut(s) 350, 452, 499
Aor13HI TCCGGA 1 cut(s) 185
AoxI GGCC 5 cut(s) 107, 334, 446, 477, 763
ApeKI GCWGC 3 cut(s) 467, 652, 661
ApoI RAATTY 3 cut(s) 539, 698, 784
ArsI GACNNNNNNTTYG 4 cut(s) 122, 154, 658, 690
Asp700I GAANNNNTTC 1 cut(s) 738
AspLEI GCGC 1 cut(s) 245
AsuHPI GGTGA 1 cut(s) 722
AsuII TTCGAA 1 cut(s) 294
BaeGI GKGCMC 1 cut(s) 374
BalI TGGCCA 2 cut(s) 336, 765
BbsI GAAGAC 2 cut(s) 28, 639
BbvI GCAGC 3 cut(s) 454, 639, 673
BccI CCATC 3 cut(s) 402, 707, 747
BceAI ACGGC 2 cut(s) 640, 776
BcgI CGANNNNNNTGC 3 cut(s) 446, 480, 769
BciT130I CCWGG 1 cut(s) 333
BclI TGATCA 2 cut(s) 67, 115
BcoDI GTCTC 3 cut(s) 350, 452, 499
BisI GCNGC 3 cut(s) 468, 653, 662
BlsI GCNGC 3 cut(s) 469, 654, 663
Bme1390I CCNGG 1 cut(s) 333
BmiI GGNNCC 1 cut(s) 184
BmrFI CCNGG 1 cut(s) 333
BmrI ACTGGG 1 cut(s) 561
BmsI GCATC 1 cut(s) 492
BmuI ACTGGG 1 cut(s) 561
BpiI GAAGAC 2 cut(s) 28, 639
BpmI CTGGAG 1 cut(s) 549
Bpu14I TTCGAA 1 cut(s) 294
BsaJI CCNNGG 1 cut(s) 104
BsaWI WCCGGW 1 cut(s) 185
Bse118I RCCGGY 1 cut(s) 479
Bse1I ACTGG 4 cut(s) 367, 491, 532, 556
Bse3DI GCAATG 1 cut(s) 259
BseAI TCCGGA 1 cut(s) 185
BseBI CCWGG 1 cut(s) 333
BseDI CCNNGG 1 cut(s) 104
BseGI GGATG 2 cut(s) 413, 758
BseMI GCAATG 1 cut(s) 259
BseNI ACTGG 4 cut(s) 367, 491, 532, 556
BseSI GKGCMC 1 cut(s) 374
BseXI GCAGC 3 cut(s) 454, 639, 673
BseYI CCCAGC 1 cut(s) 599
Bsh1236I CGCG 1 cut(s) 243
BshFI GGCC 5 cut(s) 109, 336, 448, 479, 765
BsiSI CCGG 2 cut(s) 186, 480
BsmAI GTCTC 3 cut(s) 350, 452, 499
BsnI GGCC 5 cut(s) 109, 336, 448, 479, 765
Bsp119I TTCGAA 1 cut(s) 294
Bsp1286I GDGCHC 1 cut(s) 374
Bsp13I TCCGGA 1 cut(s) 185
Bsp143I GATC 2 cut(s) 67, 115
BspACI CCGC 3 cut(s) 241, 502, 679
BspANI GGCC 5 cut(s) 109, 336, 448, 479, 765
BspEI TCCGGA 1 cut(s) 185
BspFNI CGCG 1 cut(s) 243
BspHI TCATGA 2 cut(s) 64, 88
BspLI GGNNCC 1 cut(s) 184
BspT104I TTCGAA 1 cut(s) 294
BsrDI GCAATG 1 cut(s) 259
BsrFI RCCGGY 1 cut(s) 479
BsrI ACTGG 4 cut(s) 367, 491, 532, 556
BssAI RCCGGY 1 cut(s) 479
BssECI CCNNGG 1 cut(s) 104
BssMI GATC 2 cut(s) 67, 115
BssT1I CCWWGG 1 cut(s) 104
Bst2UI CCWGG 1 cut(s) 333
Bst4CI ACNGT 1 cut(s) 724
BstBI TTCGAA 1 cut(s) 294
BstC8I GCNNGC 4 cut(s) 289, 329, 338, 481
BstF5I GGATG 2 cut(s) 413, 758
BstFNI CGCG 1 cut(s) 243
BstHHI GCGC 1 cut(s) 245
BstKTI GATC 2 cut(s) 70, 118
BstMAI GTCTC 3 cut(s) 350, 452, 499
BstMBI GATC 2 cut(s) 67, 115
BstMWI GCNNNNNNNGC 7 cut(s) 133, 249, 333, 337, 476, 658, 661
BstNI CCWGG 1 cut(s) 333
BstNSI RCATGY 2 cut(s) 158, 291
BstSCI CCNGG 1 cut(s) 331
BstSLI GKGCMC 1 cut(s) 374
BstUI CGCG 1 cut(s) 243
BstV1I GCAGC 3 cut(s) 454, 639, 673
BstV2I GAAGAC 2 cut(s) 28, 639
BstXI CCANNNNNNTGG 2 cut(s) 527, 753
BsuRI GGCC 5 cut(s) 109, 336, 448, 479, 765
BtsCI GGATG 2 cut(s) 413, 758
BtsIMutI CAGTG 1 cut(s) 374
Cac8I GCNNGC 4 cut(s) 289, 329, 338, 481
CciI TCATGA 2 cut(s) 64, 88
CfoI GCGC 1 cut(s) 245
Cfr10I RCCGGY 1 cut(s) 479
Csp6I GTAC 2 cut(s) 237, 472
CviAII CATG 7 cut(s) 65, 89, 155, 161, 288, 513, 648
CviQI GTAC 2 cut(s) 237, 472
DpnI GATC 2 cut(s) 69, 117
DpnII GATC 2 cut(s) 67, 115
EaeI YGGCCR 2 cut(s) 334, 763
Eco130I CCWWGG 1 cut(s) 104
Eco147I AGGCCT 2 cut(s) 109, 448
Eco57I CTGAAG 1 cut(s) 651
EcoRII CCWGG 1 cut(s) 331
EcoT14I CCWWGG 1 cut(s) 104
EcoT22I ATGCAT 1 cut(s) 259
ErhI CCWWGG 1 cut(s) 104
FaeI CATG 7 cut(s) 68, 92, 158, 164, 291, 516, 651
FatI CATG 7 cut(s) 64, 88, 154, 160, 287, 512, 647
FauI CCCGC 1 cut(s) 248
FbaI TGATCA 2 cut(s) 67, 115
FblI GTMKAC 1 cut(s) 212
Fnu4HI GCNGC 3 cut(s) 468, 653, 662
FokI GGATG 2 cut(s) 420, 765
Fsp4HI GCNGC 3 cut(s) 468, 653, 662
GlaI GCGC 1 cut(s) 244
GluI GCNGC 3 cut(s) 468, 653, 662
GsaI CCCAGC 1 cut(s) 603
GsuI CTGGAG 1 cut(s) 549
HaeIII GGCC 5 cut(s) 109, 336, 448, 479, 765
HapII CCGG 2 cut(s) 186, 480
HhaI GCGC 1 cut(s) 245
Hin1II CATG 7 cut(s) 68, 92, 158, 164, 291, 516, 651
Hin6I GCGC 1 cut(s) 243
HinP1I GCGC 1 cut(s) 243
HincII GTYRAC 2 cut(s) 141, 213
HindII GTYRAC 2 cut(s) 141, 213
HinfI GANTC 3 cut(s) 311, 508, 556
HpaII CCGG 2 cut(s) 186, 480
HphI GGTGA 1 cut(s) 722
Hpy166II GTNNAC 3 cut(s) 141, 213, 428
Hpy188I TCNGA 1 cut(s) 733
Hpy188III TCNNGA 4 cut(s) 65, 89, 186, 457
Hpy8I GTNNAC 3 cut(s) 141, 213, 428
HpyAV CCTTC 2 cut(s) 170, 770
HpyCH4III ACNGT 1 cut(s) 724
HpyCH4IV ACGT 1 cut(s) 615
HpyCH4V TGCA 7 cut(s) 136, 257, 287, 327, 414, 612, 661
HpyF10VI GCNNNNNNNGC 7 cut(s) 133, 249, 333, 337, 476, 658, 661
HpySE526I ACGT 1 cut(s) 615
Hsp92II CATG 7 cut(s) 68, 92, 158, 164, 291, 516, 651
HspAI GCGC 1 cut(s) 243
Kpn2I TCCGGA 1 cut(s) 185
KroI GCCGGC 1 cut(s) 479
KroNI GCCGGC 1 cut(s) 481
Ksp22I TGATCA 2 cut(s) 67, 115
Kzo9I GATC 2 cut(s) 67, 115
Lsp1109I GCAGC 3 cut(s) 454, 639, 673
LweI GCATC 1 cut(s) 492
MaeII ACGT 1 cut(s) 615
MaeIII GTNAC 2 cut(s) 352, 515
MalI GATC 2 cut(s) 69, 117
MboI GATC 2 cut(s) 67, 115
MboII GAAGA 3 cut(s) 28, 644, 729
MhlI GDGCHC 1 cut(s) 374
MlsI TGGCCA 2 cut(s) 336, 765
MluCI AATT 4 cut(s) 379, 539, 698, 784
MluNI TGGCCA 2 cut(s) 336, 765
MlyI GAGTC 1 cut(s) 305
MmeI TCCRAC 1 cut(s) 571
MnlI CCTC 8 cut(s) 96, 173, 301, 327, 384, 398, 524, 587
Mox20I TGGCCA 2 cut(s) 336, 765
Mph1103I ATGCAT 1 cut(s) 259
MroI TCCGGA 1 cut(s) 185
MroNI GCCGGC 1 cut(s) 479
MroXI GAANNNNTTC 1 cut(s) 738
MscI TGGCCA 2 cut(s) 336, 765
MseI TTAA 1 cut(s) 537
MslI CAYNNNNRTG 1 cut(s) 159
Msp20I TGGCCA 2 cut(s) 336, 765
MspA1I CMGCKG 1 cut(s) 679
MspI CCGG 2 cut(s) 186, 480
MspR9I CCNGG 1 cut(s) 333
MvaI CCWGG 1 cut(s) 333
MvnI CGCG 1 cut(s) 243
MwoI GCNNNNNNNGC 7 cut(s) 133, 249, 333, 337, 476, 658, 661
NaeI GCCGGC 1 cut(s) 481
NdeII GATC 2 cut(s) 67, 115
NgoMIV GCCGGC 1 cut(s) 479
NlaIII CATG 7 cut(s) 68, 92, 158, 164, 291, 516, 651
NlaIV GGNNCC 1 cut(s) 184
NmuCI GTSAC 1 cut(s) 515
NsiI ATGCAT 1 cut(s) 259
NspI RCATGY 2 cut(s) 158, 291
NspV TTCGAA 1 cut(s) 294
PaeI GCATGC 1 cut(s) 291
PagI TCATGA 2 cut(s) 64, 88
PceI AGGCCT 2 cut(s) 109, 448
PdiI GCCGGC 1 cut(s) 481
PdmI GAANNNNTTC 1 cut(s) 738
PfeI GAWTC 2 cut(s) 508, 556
PkrI GCNGC 3 cut(s) 469, 654, 663
PleI GAGTC 1 cut(s) 305
PpsI GAGTC 1 cut(s) 305
Psp1406I AACGTT 1 cut(s) 615
Psp6I CCWGG 1 cut(s) 331
PspFI CCCAGC 1 cut(s) 599
PspGI CCWGG 1 cut(s) 331
PspN4I GGNNCC 1 cut(s) 184
RsaI GTAC 2 cut(s) 238, 473
RsaNI GTAC 2 cut(s) 237, 472
RseI CAYNNNNRTG 1 cut(s) 159
SalI GTCGAC 1 cut(s) 211
SaqAI TTAA 1 cut(s) 537
SatI GCNGC 3 cut(s) 468, 653, 662
Sau3AI GATC 2 cut(s) 67, 115
SchI GAGTC 1 cut(s) 305
ScrFI CCNGG 1 cut(s) 333
SduI GDGCHC 1 cut(s) 374
SetI ASST 5 cut(s) 16, 184, 598, 618, 631
SfaNI GCATC 1 cut(s) 492
SfuI TTCGAA 1 cut(s) 294
SmiMI CAYNNNNRTG 1 cut(s) 159
SphI GCATGC 1 cut(s) 291
Sse9I AATT 4 cut(s) 379, 539, 698, 784
SseBI AGGCCT 2 cut(s) 109, 448
SsiI CCGC 3 cut(s) 241, 502, 679
StuI AGGCCT 2 cut(s) 109, 448
StyD4I CCNGG 1 cut(s) 331
StyI CCWWGG 1 cut(s) 104
TaaI ACNGT 1 cut(s) 724
TaiI ACGT 1 cut(s) 618
TaqI TCGA 4 cut(s) 212, 294, 435, 456
TasI AATT 4 cut(s) 379, 539, 698, 784
TfiI GAWTC 2 cut(s) 508, 556
Tru1I TTAA 1 cut(s) 537
Tru9I TTAA 1 cut(s) 537
TscAI CASTG 1 cut(s) 374
TseFI GTSAC 1 cut(s) 515
TseI GCWGC 3 cut(s) 467, 652, 661
Tsp45I GTSAC 1 cut(s) 515
TspDTI ATGAA 3 cut(s) 189, 585, 789
TspGWI ACGGA 1 cut(s) 417
TspRI CASTG 1 cut(s) 374
XapI RAATTY 3 cut(s) 539, 698, 784
XceI RCATGY 2 cut(s) 158, 291
XmiI GTMKAC 1 cut(s) 212
XmnI GAANNNNTTC 1 cut(s) 738
Zsp2I ATGCAT 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.