Rh3DG186800

expansin-A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0017331
Physical Location & Seq
Forward (+)
16619689 .. 16621611
1923 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG186800.1

Sequence Viewer

Length: 798 bp
ATGGAAATGCCTTTGAGAGCACTGTTTGGGTCATTCCTTGTCTTGTTTTTTGTTGGACATGCTGTCCATGGTCATGATGGTCAAAAGTTCCAGGCAGGTGAATGGAAACATGCTCATGCCACACACTATGAAGGAAGCTCCGACACATTTGGTGGAGCTTGTGGATACCATGATGTCGTTAAAGAAGGCTATGGCCTGGAGACAGTAGCATTGAGCAATGCATTGTTCAACAAGGGGGAGATGTGTGGAGCATGCTTCGAAATCAAATGCGAGGACTCGCCTGAATGGTGCAAGCCTGGCCAGCCAAGTCTCATTGTTACAGGAACCAACCAGTGCCCTCCGAATTACGACCAGCCGAGTGATAATGGAGGATGGTGCAACGAACCCCGTGAACATTTCGACATAGCAAGGCCAGTGTTCGAGACGATTGCCGCGTACAAGGCCGGCATAGTTCCAGTCTCATACCGCAGGATTCCATGTGATCATAAGGAAGGAGGAATTAAATTTACAATAACTGGGAATCCTTATTTCAATGAAGTGTTGGTGTGGAACGTAGGAGGTGCTGGGGATATTTGCCACGTCGAAGTGAAAACTGAAGAGCAACCGGATAAGTGGATACCGATGGAGCGAATGTGGGGTCAACGGTGGATTTACAACGGAAAATTGTGTGAGAGTCAAGGCGCGCTTACCTTCCGAGTGACAGGGGGTGATGGAAGAAGCGTAACCTGTGACAATGTTGCCCCAAAGGGTTGGCAGTTTGGCCAAACTTATCAAGCCAAGAATAATTTCGAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

265

Amino Acids

29.48

Weight (kDa)

5.68

Isoelectric Point (pI)

34.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXPB1_D1 PF22514 39 - 162 3.7e-09 EXPB1-like domain 1
DPBB_1 PF03330 68 - 155 9e-18 Lytic transglycolase
Expansin_C PF01357 167 - 248 2.9e-24 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017331)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g16911
rosa_chinensis RchiOBHm_Chr2g0140121 RchiOBHm_Chr3g0470941
rosa_laevigata RLG00000024231
rosa_multiflora Rmu_sc0005264.1_g000003
rosa_roxburghii Rroxscaffold_6G00410390
rosa_rugosa Rorug03G0114100
rosa_samantha Rh2AG411900 Rh2CG398000 Rh3BG189400 Rh3DG186800
rosa_wichuraiana Rw2G033750 Rw3G015420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 86
Acc36I ACCTGC 1 cut(s) 86
AccII CGCG 2 cut(s) 434, 683
AciI CCGC 2 cut(s) 432, 466
AcoI YGGCCR 2 cut(s) 298, 760
AcsI RAATTY 1 cut(s) 503
AcuI CTGAAG 1 cut(s) 615
AfaI GTAC 1 cut(s) 437
AgsI TTSAA 2 cut(s) 229, 532
AhdI GACNNNNNGTC 1 cut(s) 62
AjiI CACGTC 1 cut(s) 580
AjnI CCWGG 3 cut(s) 90, 195, 295
AjuI GAANNNNNNNTTGG 1 cut(s) 770
AluBI AGCT 2 cut(s) 138, 158
AluI AGCT 2 cut(s) 138, 158
Alw21I GWGCWC 1 cut(s) 22
Alw26I GTCTC 4 cut(s) 194, 314, 416, 463
AoxI GGCC 5 cut(s) 193, 298, 410, 441, 760
ApoI RAATTY 1 cut(s) 503
ArsI GACNNNNNNTTYG 2 cut(s) 622, 654
Asp700I GAANNNNTTC 1 cut(s) 785
AspLEI GCGC 2 cut(s) 683, 685
AsuHPI GGTGA 2 cut(s) 110, 719
AsuII TTCGAA 2 cut(s) 258, 789
BaeGI GKGCMC 1 cut(s) 338
BalI TGGCCA 2 cut(s) 300, 762
Bbv12I GWGCWC 1 cut(s) 22
BccI CCATC 4 cut(s) 71, 366, 616, 704
BcgI CGANNNNNNTGC 2 cut(s) 410, 444
BciT130I CCWGG 3 cut(s) 92, 197, 297
BciVI GTATCC 2 cut(s) 158, 609
BclI TGATCA 1 cut(s) 481
BcoDI GTCTC 4 cut(s) 194, 314, 416, 463
BfuAI ACCTGC 1 cut(s) 86
BfuI GTATCC 2 cut(s) 158, 609
BisI GCNGC 1 cut(s) 432
BlsI GCNGC 1 cut(s) 433
Bme1390I CCNGG 3 cut(s) 92, 197, 297
BmeRI GACNNNNNGTC 1 cut(s) 62
BmgBI CACGTC 1 cut(s) 580
BmiI GGNNCC 1 cut(s) 325
BmrFI CCNGG 3 cut(s) 92, 197, 297
BmrI ACTGGG 1 cut(s) 525
BmuI ACTGGG 1 cut(s) 525
BpmI CTGGAG 1 cut(s) 218
Bpu14I TTCGAA 2 cut(s) 258, 789
BsaJI CCNNGG 1 cut(s) 67
BsaWI WCCGGW 1 cut(s) 604
BsaXI ACNNNNNCTCC 2 cut(s) 617, 647
Bse118I RCCGGY 1 cut(s) 443
Bse1I ACTGG 4 cut(s) 331, 413, 455, 520
Bse3DI GCAATG 1 cut(s) 223
BseBI CCWGG 3 cut(s) 92, 197, 297
BseDI CCNNGG 1 cut(s) 67
BseGI GGATG 1 cut(s) 377
BseMI GCAATG 1 cut(s) 223
BseNI ACTGG 4 cut(s) 331, 413, 455, 520
BsePI GCGCGC 1 cut(s) 681
BseSI GKGCMC 1 cut(s) 338
BseYI CCCAGC 1 cut(s) 563
Bsh1236I CGCG 2 cut(s) 434, 683
BshFI GGCC 5 cut(s) 195, 300, 412, 443, 762
BsiHKAI GWGCWC 1 cut(s) 22
BsiSI CCGG 2 cut(s) 444, 605
BsmAI GTCTC 4 cut(s) 194, 314, 416, 463
BsmBI CGTCTC 1 cut(s) 416
BsnI GGCC 5 cut(s) 195, 300, 412, 443, 762
Bsp119I TTCGAA 2 cut(s) 258, 789
Bsp1286I GDGCHC 2 cut(s) 22, 338
Bsp143I GATC 1 cut(s) 481
Bsp19I CCATGG 1 cut(s) 67
BspACI CCGC 2 cut(s) 432, 466
BspANI GGCC 5 cut(s) 195, 300, 412, 443, 762
BspFNI CGCG 2 cut(s) 434, 683
BspHI TCATGA 1 cut(s) 73
BspLI GGNNCC 1 cut(s) 325
BspMI ACCTGC 1 cut(s) 86
BspQI GCTCTTC 1 cut(s) 591
BspT104I TTCGAA 2 cut(s) 258, 789
BsrDI GCAATG 1 cut(s) 223
BsrFI RCCGGY 1 cut(s) 443
BsrI ACTGG 4 cut(s) 331, 413, 455, 520
BssAI RCCGGY 1 cut(s) 443
BssECI CCNNGG 1 cut(s) 67
BssHII GCGCGC 1 cut(s) 681
BssMI GATC 1 cut(s) 481
BssT1I CCWWGG 1 cut(s) 67
Bst2UI CCWGG 3 cut(s) 92, 197, 297
Bst4CI ACNGT 3 cut(s) 24, 205, 645
Bst6I CTCTTC 1 cut(s) 591
BstBI TTCGAA 2 cut(s) 258, 789
BstC8I GCNNGC 5 cut(s) 253, 293, 302, 445, 683
BstDSI CCRYGG 1 cut(s) 67
BstF5I GGATG 1 cut(s) 377
BstFNI CGCG 2 cut(s) 434, 683
BstHHI GCGC 2 cut(s) 683, 685
BstKTI GATC 1 cut(s) 484
BstMAI GTCTC 4 cut(s) 194, 314, 416, 463
BstMBI GATC 1 cut(s) 481
BstMWI GCNNNNNNNGC 3 cut(s) 297, 301, 440
BstNI CCWGG 3 cut(s) 92, 197, 297
BstNSI RCATGY 3 cut(s) 62, 113, 255
BstSCI CCNGG 3 cut(s) 90, 195, 295
BstSLI GKGCMC 1 cut(s) 338
BstUI CGCG 2 cut(s) 434, 683
BstXI CCANNNNNNTGG 1 cut(s) 750
BsuI GTATCC 2 cut(s) 158, 609
BsuRI GGCC 5 cut(s) 195, 300, 412, 443, 762
BtgI CCRYGG 1 cut(s) 67
BtrI CACGTC 1 cut(s) 580
BtsCI GGATG 1 cut(s) 377
BtsIMutI CAGTG 3 cut(s) 20, 338, 420
BveI ACCTGC 1 cut(s) 86
Cac8I GCNNGC 5 cut(s) 253, 293, 302, 445, 683
CciI TCATGA 1 cut(s) 73
CfoI GCGC 2 cut(s) 683, 685
Cfr10I RCCGGY 1 cut(s) 443
Csp6I GTAC 1 cut(s) 436
CviAII CATG 8 cut(s) 59, 68, 74, 110, 116, 170, 252, 477
CviQI GTAC 1 cut(s) 436
DpnI GATC 1 cut(s) 483
DpnII GATC 1 cut(s) 481
DriI GACNNNNNGTC 1 cut(s) 62
EaeI YGGCCR 2 cut(s) 298, 760
Eam1104I CTCTTC 1 cut(s) 591
Eam1105I GACNNNNNGTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 591
Eco130I CCWWGG 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 615
EcoRII CCWGG 3 cut(s) 90, 195, 295
EcoT14I CCWWGG 1 cut(s) 67
EcoT22I ATGCAT 1 cut(s) 223
ErhI CCWWGG 1 cut(s) 67
Esp3I CGTCTC 1 cut(s) 416
FaeI CATG 8 cut(s) 62, 71, 77, 113, 119, 173, 255, 480
FalI AAGNNNNNCTT 2 cut(s) 669, 701
FatI CATG 8 cut(s) 58, 67, 73, 109, 115, 169, 251, 476
FbaI TGATCA 1 cut(s) 481
Fnu4HI GCNGC 1 cut(s) 432
FokI GGATG 1 cut(s) 384
Fsp4HI GCNGC 1 cut(s) 432
GlaI GCGC 2 cut(s) 682, 684
GluI GCNGC 1 cut(s) 432
GsaI CCCAGC 1 cut(s) 567
GsuI CTGGAG 1 cut(s) 218
HaeIII GGCC 5 cut(s) 195, 300, 412, 443, 762
HapII CCGG 2 cut(s) 444, 605
HhaI GCGC 2 cut(s) 683, 685
Hin1II CATG 8 cut(s) 62, 71, 77, 113, 119, 173, 255, 480
Hin6I GCGC 2 cut(s) 681, 683
HinP1I GCGC 2 cut(s) 681, 683
HincII GTYRAC 1 cut(s) 641
HindII GTYRAC 1 cut(s) 641
HinfI GANTC 4 cut(s) 275, 472, 520, 673
HpaII CCGG 2 cut(s) 444, 605
HphI GGTGA 2 cut(s) 110, 719
Hpy166II GTNNAC 2 cut(s) 392, 641
Hpy188I TCNGA 3 cut(s) 142, 342, 695
Hpy188III TCNNGA 2 cut(s) 74, 421
Hpy8I GTNNAC 2 cut(s) 392, 641
Hpy99I CGWCG 1 cut(s) 584
HpyAV CCTTC 4 cut(s) 125, 179, 485, 700
HpyCH4III ACNGT 3 cut(s) 24, 205, 645
HpyCH4IV ACGT 2 cut(s) 552, 579
HpyCH4V TGCA 3 cut(s) 221, 291, 378
HpyF10VI GCNNNNNNNGC 3 cut(s) 297, 301, 440
HpySE526I ACGT 2 cut(s) 552, 579
Hsp92II CATG 8 cut(s) 62, 71, 77, 113, 119, 173, 255, 480
HspAI GCGC 2 cut(s) 681, 683
KroI GCCGGC 1 cut(s) 443
KroNI GCCGGC 1 cut(s) 445
Ksp22I TGATCA 1 cut(s) 481
Kzo9I GATC 1 cut(s) 481
LguI GCTCTTC 1 cut(s) 591
LmnI GCTCC 4 cut(s) 143, 155, 248, 625
MaeII ACGT 2 cut(s) 552, 579
MaeIII GTNAC 4 cut(s) 316, 697, 721, 728
MalI GATC 1 cut(s) 483
MboI GATC 1 cut(s) 481
MboII GAAGA 2 cut(s) 608, 726
MhlI GDGCHC 2 cut(s) 22, 338
MlsI TGGCCA 2 cut(s) 300, 762
MluCI AATT 5 cut(s) 343, 498, 503, 662, 784
MluNI TGGCCA 2 cut(s) 300, 762
MlyI GAGTC 2 cut(s) 269, 682
MmeI TCCRAC 2 cut(s) 34, 165
MnlI CCTC 5 cut(s) 265, 348, 362, 488, 551
Mox20I TGGCCA 2 cut(s) 300, 762
Mph1103I ATGCAT 1 cut(s) 223
MroNI GCCGGC 1 cut(s) 443
MroXI GAANNNNTTC 1 cut(s) 785
MscI TGGCCA 2 cut(s) 300, 762
MseI TTAA 2 cut(s) 180, 501
MslI CAYNNNNRTG 2 cut(s) 72, 114
Msp20I TGGCCA 2 cut(s) 300, 762
MspI CCGG 2 cut(s) 444, 605
MspR9I CCNGG 3 cut(s) 92, 197, 297
MvaI CCWGG 3 cut(s) 92, 197, 297
MvnI CGCG 2 cut(s) 434, 683
MwoI GCNNNNNNNGC 3 cut(s) 297, 301, 440
NaeI GCCGGC 1 cut(s) 445
NcoI CCATGG 1 cut(s) 67
NdeII GATC 1 cut(s) 481
NgoMIV GCCGGC 1 cut(s) 443
NlaIII CATG 8 cut(s) 62, 71, 77, 113, 119, 173, 255, 480
NlaIV GGNNCC 1 cut(s) 325
NmeAIII GCCGAG 1 cut(s) 381
NmuCI GTSAC 2 cut(s) 697, 728
NsiI ATGCAT 1 cut(s) 223
NspI RCATGY 3 cut(s) 62, 113, 255
NspV TTCGAA 2 cut(s) 258, 789
PaeI GCATGC 1 cut(s) 255
PagI TCATGA 1 cut(s) 73
PaqCI CACCTGC 1 cut(s) 86
PauI GCGCGC 1 cut(s) 681
PciSI GCTCTTC 1 cut(s) 591
PcsI WCGNNNNNNNCGW 1 cut(s) 431
PdiI GCCGGC 1 cut(s) 445
PdmI GAANNNNTTC 1 cut(s) 785
PfeI GAWTC 2 cut(s) 472, 520
PkrI GCNGC 1 cut(s) 433
PleI GAGTC 2 cut(s) 269, 681
PpsI GAGTC 2 cut(s) 269, 681
Psp6I CCWGG 3 cut(s) 90, 195, 295
PspFI CCCAGC 1 cut(s) 563
PspGI CCWGG 3 cut(s) 90, 195, 295
PspN4I GGNNCC 1 cut(s) 325
PteI GCGCGC 1 cut(s) 681
RsaI GTAC 1 cut(s) 437
RsaNI GTAC 1 cut(s) 436
RseI CAYNNNNRTG 2 cut(s) 72, 114
SapI GCTCTTC 1 cut(s) 591
SaqAI TTAA 2 cut(s) 180, 501
SatI GCNGC 1 cut(s) 432
Sau3AI GATC 1 cut(s) 481
SchI GAGTC 2 cut(s) 269, 682
ScrFI CCNGG 3 cut(s) 92, 197, 297
SduI GDGCHC 2 cut(s) 22, 338
SetI ASST 8 cut(s) 100, 140, 160, 555, 562, 582, 692, 728
SfuI TTCGAA 2 cut(s) 258, 789
SmiMI CAYNNNNRTG 2 cut(s) 72, 114
SphI GCATGC 1 cut(s) 255
Sse9I AATT 5 cut(s) 343, 498, 503, 662, 784
SsiI CCGC 2 cut(s) 432, 466
StyD4I CCNGG 3 cut(s) 90, 195, 295
StyI CCWWGG 1 cut(s) 67
TaaI ACNGT 3 cut(s) 24, 205, 645
TaiI ACGT 2 cut(s) 555, 582
TaqI TCGA 5 cut(s) 258, 399, 420, 582, 789
TasI AATT 5 cut(s) 343, 498, 503, 662, 784
TauI GCSGC 1 cut(s) 434
TfiI GAWTC 2 cut(s) 472, 520
Tru1I TTAA 2 cut(s) 180, 501
Tru9I TTAA 2 cut(s) 180, 501
TscAI CASTG 3 cut(s) 27, 338, 420
TseFI GTSAC 2 cut(s) 697, 728
Tsp45I GTSAC 2 cut(s) 697, 728
TspDTI ATGAA 2 cut(s) 144, 549
TspGWI ACGGA 1 cut(s) 672
TspRI CASTG 3 cut(s) 27, 338, 420
XapI RAATTY 1 cut(s) 503
XceI RCATGY 3 cut(s) 62, 113, 255
XcmI CCANNNNNNNNNTGG 1 cut(s) 74
XmnI GAANNNNTTC 1 cut(s) 785
Zsp2I ATGCAT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.