Rh2CG398000

expansin-A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0017331
Physical Location & Seq
Forward (+)
54061042 .. 54064279
3238 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG398000.1

Sequence Viewer

Length: 909 bp
ATGTCTTTGAGAGCTTTGTGTTGGTCGTTCCTTGTCTTCTTTTTTGCTGTCTATGTCAATGCTCATGATCATCATAAGCCAAAGCCTCATGACCAGCATAAGCCAAAGCCTCATGACCAGCATAAGCCAAGGCCTGATGATCAGCAGAAGCCAAGGCCAGATGATCAGCAGAAGCCAACGTTTGATGATCAGCAGAAGCCAAAGTTGCAGTCAACTGAATGGAAACATGCTCATGCCACATTCTATGAAGGAGGTTCCGGAACATTTGGTGGAGCTTGTGGATACCATGATGTAGTTCAAGAAGGTTATGGCCTGGAGACAGTAGCATTGAGCAATGCATTGTTCAATAAGGGGGAGATGTGTGGTGCATGCTTCGAAATCAAATGCGAGGACTCGCCTCAATGGTGCAAGCCTGGCCAGCCAAGTCTTATTGTTACAGCAACCAACCAGTGCCCTCCTAATTACAACCAACCGAGTGATAACGGAGGATGGTGCAACGAACCCCGTGAACATTTCGACATAGCAAGGCCTGTGTTCGAGACTATTGCTGCGTACAAGGCCGGCATCGTTCCAGTCTCATACCGCAGGATTCCATGTGACCACAAGACTGGAGGCATTAAATTTACAATAACTGGGAATCCTTATTTCAATGAAGTGTTGGTGTGGAATGTTGGAGGTGCTGGGGATGTATGCAACGTCGAAGTGAAACCTGAAGACCAACCGAATACATGGCTGCCGCTGCAGCGAATGTGGGGTCAGCGGTGGATTTACAATGGAAAATTGTGCGAGCAAGGCGCGCTTAGCTTTCGAGTGACATCGGGTGATGGAAGAACCGTAATCTCTGAAAATGTTCTCCCAAAGGGATGGCAGTTTGGCCAGACTTATGAAGGCAAGAATTTCGCCGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

302

Amino Acids

34.04

Weight (kDa)

6.63

Isoelectric Point (pI)

35.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXPB1_D1 PF22514 79 - 201 3.5e-09 EXPB1-like domain 1
DPBB_1 PF03330 107 - 194 3.7e-19 Lytic transglycolase
Expansin_C PF01357 206 - 286 4e-25 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017331)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g16911
rosa_chinensis RchiOBHm_Chr2g0140121 RchiOBHm_Chr3g0470941
rosa_laevigata RLG00000024231
rosa_multiflora Rmu_sc0005264.1_g000003
rosa_roxburghii Rroxscaffold_6G00410390
rosa_rugosa Rorug03G0114100
rosa_samantha Rh2AG411900 Rh2CG398000 Rh3BG189400 Rh3DG186800
rosa_wichuraiana Rw2G033750 Rw3G015420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 797
AccIII TCCGGA 1 cut(s) 257
AciI CCGC 3 cut(s) 583, 737, 760
AclI AACGTT 1 cut(s) 179
AcoI YGGCCR 2 cut(s) 415, 874
AcsI RAATTY 2 cut(s) 620, 895
AcuI CTGAAG 1 cut(s) 732
AfaI GTAC 1 cut(s) 554
AgsI TTSAA 3 cut(s) 299, 346, 649
AjnI CCWGG 2 cut(s) 312, 412
AluBI AGCT 3 cut(s) 14, 275, 804
AluI AGCT 3 cut(s) 14, 275, 804
Alw26I GTCTC 3 cut(s) 311, 533, 580
Aor13HI TCCGGA 1 cut(s) 257
AoxI GGCC 7 cut(s) 131, 155, 310, 415, 527, 558, 874
ApeKI GCWGC 4 cut(s) 548, 733, 739, 742
ApoI RAATTY 2 cut(s) 620, 895
ArsI GACNNNNNNTTYG 4 cut(s) 194, 226, 739, 771
Asp700I GAANNNNTTC 1 cut(s) 849
AspLEI GCGC 2 cut(s) 797, 799
AsuHPI GGTGA 1 cut(s) 833
AsuII TTCGAA 1 cut(s) 375
BaeGI GKGCMC 1 cut(s) 455
BalI TGGCCA 2 cut(s) 417, 876
BbsI GAAGAC 2 cut(s) 28, 720
BbvI GCAGC 4 cut(s) 535, 720, 726, 754
BccI CCATC 3 cut(s) 483, 818, 858
BceAI ACGGC 1 cut(s) 887
BcgI CGANNNNNNTGC 3 cut(s) 527, 561, 880
BciT130I CCWGG 2 cut(s) 314, 414
BciVI GTATCC 1 cut(s) 275
BclI TGATCA 4 cut(s) 67, 139, 163, 187
BcoDI GTCTC 3 cut(s) 311, 533, 580
BfmI CTRYAG 1 cut(s) 740
BfuI GTATCC 1 cut(s) 275
BisI GCNGC 5 cut(s) 549, 734, 737, 740, 743
BlpI GCTNAGC 1 cut(s) 800
BlsI GCNGC 5 cut(s) 550, 735, 738, 741, 744
Bme1390I CCNGG 2 cut(s) 314, 414
BmiI GGNNCC 1 cut(s) 256
BmrFI CCNGG 2 cut(s) 314, 414
BmrI ACTGGG 1 cut(s) 642
BmsI GCATC 1 cut(s) 573
BmuI ACTGGG 1 cut(s) 642
BpiI GAAGAC 2 cut(s) 28, 720
BpmI CTGGAG 2 cut(s) 335, 630
Bpu1102I GCTNAGC 1 cut(s) 800
Bpu14I TTCGAA 1 cut(s) 375
BsaJI CCNNGG 2 cut(s) 128, 152
BsaWI WCCGGW 1 cut(s) 257
Bse118I RCCGGY 1 cut(s) 560
Bse1I ACTGG 4 cut(s) 448, 572, 613, 637
Bse3DI GCAATG 1 cut(s) 340
BseAI TCCGGA 1 cut(s) 257
BseBI CCWGG 2 cut(s) 314, 414
BseDI CCNNGG 2 cut(s) 128, 152
BseGI GGATG 3 cut(s) 494, 691, 869
BseMI GCAATG 1 cut(s) 340
BseNI ACTGG 4 cut(s) 448, 572, 613, 637
BsePI GCGCGC 1 cut(s) 795
BseSI GKGCMC 1 cut(s) 455
BseXI GCAGC 4 cut(s) 535, 720, 726, 754
BseYI CCCAGC 1 cut(s) 680
Bsh1236I CGCG 1 cut(s) 797
BshFI GGCC 7 cut(s) 133, 157, 312, 417, 529, 560, 876
BsiSI CCGG 2 cut(s) 258, 561
BsmAI GTCTC 3 cut(s) 311, 533, 580
BsnI GGCC 7 cut(s) 133, 157, 312, 417, 529, 560, 876
Bsp119I TTCGAA 1 cut(s) 375
Bsp1286I GDGCHC 1 cut(s) 455
Bsp13I TCCGGA 1 cut(s) 257
Bsp143I GATC 4 cut(s) 67, 139, 163, 187
Bsp1720I GCTNAGC 1 cut(s) 800
BspACI CCGC 3 cut(s) 583, 737, 760
BspANI GGCC 7 cut(s) 133, 157, 312, 417, 529, 560, 876
BspEI TCCGGA 1 cut(s) 257
BspFNI CGCG 1 cut(s) 797
BspHI TCATGA 3 cut(s) 64, 88, 112
BspLI GGNNCC 1 cut(s) 256
BspMAI CTGCAG 1 cut(s) 744
BspT104I TTCGAA 1 cut(s) 375
BsrDI GCAATG 1 cut(s) 340
BsrFI RCCGGY 1 cut(s) 560
BsrI ACTGG 4 cut(s) 448, 572, 613, 637
BssAI RCCGGY 1 cut(s) 560
BssECI CCNNGG 2 cut(s) 128, 152
BssHII GCGCGC 1 cut(s) 795
BssMI GATC 4 cut(s) 67, 139, 163, 187
BssT1I CCWWGG 2 cut(s) 128, 152
Bst2UI CCWGG 2 cut(s) 314, 414
Bst4CI ACNGT 2 cut(s) 322, 835
BstBI TTCGAA 1 cut(s) 375
BstC8I GCNNGC 6 cut(s) 370, 410, 419, 562, 788, 797
BstDEI CTNAG 1 cut(s) 800
BstF5I GGATG 3 cut(s) 494, 691, 869
BstFNI CGCG 1 cut(s) 797
BstHHI GCGC 2 cut(s) 797, 799
BstKTI GATC 4 cut(s) 70, 142, 166, 190
BstMAI GTCTC 3 cut(s) 311, 533, 580
BstMBI GATC 4 cut(s) 67, 139, 163, 187
BstMWI GCNNNNNNNGC 9 cut(s) 205, 414, 418, 557, 739, 742, 792, 796, 801
BstNI CCWGG 2 cut(s) 314, 414
BstNSI RCATGY 2 cut(s) 230, 372
BstSCI CCNGG 2 cut(s) 312, 412
BstSFI CTRYAG 1 cut(s) 740
BstSLI GKGCMC 1 cut(s) 455
BstUI CGCG 1 cut(s) 797
BstV1I GCAGC 4 cut(s) 535, 720, 726, 754
BstV2I GAAGAC 2 cut(s) 28, 720
BstXI CCANNNNNNTGG 2 cut(s) 608, 864
BsuI GTATCC 1 cut(s) 275
BsuRI GGCC 7 cut(s) 133, 157, 312, 417, 529, 560, 876
BtsCI GGATG 3 cut(s) 494, 691, 869
BtsIMutI CAGTG 1 cut(s) 455
Cac8I GCNNGC 6 cut(s) 370, 410, 419, 562, 788, 797
CciI TCATGA 3 cut(s) 64, 88, 112
CfoI GCGC 2 cut(s) 797, 799
Cfr10I RCCGGY 1 cut(s) 560
Csp6I GTAC 1 cut(s) 553
CviAII CATG 9 cut(s) 65, 89, 113, 227, 233, 287, 369, 594, 729
CviQI GTAC 1 cut(s) 553
DdeI CTNAG 1 cut(s) 800
DpnI GATC 4 cut(s) 69, 141, 165, 189
DpnII GATC 4 cut(s) 67, 139, 163, 187
EaeI YGGCCR 2 cut(s) 415, 874
Eco130I CCWWGG 2 cut(s) 128, 152
Eco147I AGGCCT 2 cut(s) 133, 529
Eco57I CTGAAG 1 cut(s) 732
EcoRII CCWGG 2 cut(s) 312, 412
EcoT14I CCWWGG 2 cut(s) 128, 152
EcoT22I ATGCAT 1 cut(s) 340
ErhI CCWWGG 2 cut(s) 128, 152
FaeI CATG 9 cut(s) 68, 92, 116, 230, 236, 290, 372, 597, 732
FalI AAGNNNNNCTT 2 cut(s) 783, 815
FatI CATG 9 cut(s) 64, 88, 112, 226, 232, 286, 368, 593, 728
FbaI TGATCA 4 cut(s) 67, 139, 163, 187
Fnu4HI GCNGC 5 cut(s) 549, 734, 737, 740, 743
FokI GGATG 3 cut(s) 501, 698, 876
Fsp4HI GCNGC 5 cut(s) 549, 734, 737, 740, 743
GlaI GCGC 2 cut(s) 796, 798
GluI GCNGC 5 cut(s) 549, 734, 737, 740, 743
GsaI CCCAGC 1 cut(s) 684
GsuI CTGGAG 2 cut(s) 335, 630
HaeIII GGCC 7 cut(s) 133, 157, 312, 417, 529, 560, 876
HapII CCGG 2 cut(s) 258, 561
HhaI GCGC 2 cut(s) 797, 799
Hin1II CATG 9 cut(s) 68, 92, 116, 230, 236, 290, 372, 597, 732
Hin6I GCGC 2 cut(s) 795, 797
HinP1I GCGC 2 cut(s) 795, 797
HincII GTYRAC 1 cut(s) 213
HindII GTYRAC 1 cut(s) 213
HinfI GANTC 3 cut(s) 392, 589, 637
HpaII CCGG 2 cut(s) 258, 561
HphI GGTGA 1 cut(s) 833
Hpy166II GTNNAC 2 cut(s) 213, 509
Hpy188I TCNGA 1 cut(s) 844
Hpy188III TCNNGA 6 cut(s) 65, 89, 113, 258, 299, 538
Hpy8I GTNNAC 2 cut(s) 213, 509
Hpy99I CGWCG 1 cut(s) 701
HpyAV CCTTC 3 cut(s) 242, 296, 881
HpyCH4III ACNGT 2 cut(s) 322, 835
HpyCH4IV ACGT 2 cut(s) 179, 696
HpyCH4V TGCA 7 cut(s) 208, 338, 368, 408, 495, 693, 742
HpyF10VI GCNNNNNNNGC 9 cut(s) 205, 414, 418, 557, 739, 742, 792, 796, 801
HpyF3I CTNAG 1 cut(s) 800
HpySE526I ACGT 2 cut(s) 179, 696
Hsp92II CATG 9 cut(s) 68, 92, 116, 230, 236, 290, 372, 597, 732
HspAI GCGC 2 cut(s) 795, 797
Kpn2I TCCGGA 1 cut(s) 257
KroI GCCGGC 1 cut(s) 560
KroNI GCCGGC 1 cut(s) 562
Ksp22I TGATCA 4 cut(s) 67, 139, 163, 187
Kzo9I GATC 4 cut(s) 67, 139, 163, 187
LmnI GCTCC 1 cut(s) 272
Lsp1109I GCAGC 4 cut(s) 535, 720, 726, 754
LweI GCATC 1 cut(s) 573
MaeII ACGT 2 cut(s) 179, 696
MaeIII GTNAC 3 cut(s) 433, 596, 811
MalI GATC 4 cut(s) 69, 141, 165, 189
MboI GATC 4 cut(s) 67, 139, 163, 187
MboII GAAGA 3 cut(s) 28, 725, 840
MhlI GDGCHC 1 cut(s) 455
MlsI TGGCCA 2 cut(s) 417, 876
MluCI AATT 4 cut(s) 460, 620, 779, 895
MluNI TGGCCA 2 cut(s) 417, 876
MlyI GAGTC 1 cut(s) 386
MmeI TCCRAC 1 cut(s) 652
MnlI CCTC 9 cut(s) 96, 120, 245, 382, 408, 465, 479, 605, 668
Mox20I TGGCCA 2 cut(s) 417, 876
Mph1103I ATGCAT 1 cut(s) 340
MroI TCCGGA 1 cut(s) 257
MroNI GCCGGC 1 cut(s) 560
MroXI GAANNNNTTC 1 cut(s) 849
MscI TGGCCA 2 cut(s) 417, 876
MseI TTAA 1 cut(s) 618
MslI CAYNNNNRTG 1 cut(s) 231
Msp20I TGGCCA 2 cut(s) 417, 876
MspA1I CMGCKG 2 cut(s) 739, 760
MspI CCGG 2 cut(s) 258, 561
MspR9I CCNGG 2 cut(s) 314, 414
MvaI CCWGG 2 cut(s) 314, 414
MvnI CGCG 1 cut(s) 797
MwoI GCNNNNNNNGC 9 cut(s) 205, 414, 418, 557, 739, 742, 792, 796, 801
NaeI GCCGGC 1 cut(s) 562
NdeII GATC 4 cut(s) 67, 139, 163, 187
NgoMIV GCCGGC 1 cut(s) 560
NlaIII CATG 9 cut(s) 68, 92, 116, 230, 236, 290, 372, 597, 732
NlaIV GGNNCC 1 cut(s) 256
NmuCI GTSAC 2 cut(s) 596, 811
NsiI ATGCAT 1 cut(s) 340
NspI RCATGY 2 cut(s) 230, 372
NspV TTCGAA 1 cut(s) 375
PaeI GCATGC 1 cut(s) 372
PagI TCATGA 3 cut(s) 64, 88, 112
PauI GCGCGC 1 cut(s) 795
PceI AGGCCT 2 cut(s) 133, 529
PdiI GCCGGC 1 cut(s) 562
PdmI GAANNNNTTC 1 cut(s) 849
PfeI GAWTC 2 cut(s) 589, 637
PkrI GCNGC 5 cut(s) 550, 735, 738, 741, 744
PleI GAGTC 1 cut(s) 386
PpsI GAGTC 1 cut(s) 386
Psp1406I AACGTT 1 cut(s) 179
Psp6I CCWGG 2 cut(s) 312, 412
PspFI CCCAGC 1 cut(s) 680
PspGI CCWGG 2 cut(s) 312, 412
PspN4I GGNNCC 1 cut(s) 256
PstI CTGCAG 1 cut(s) 744
PteI GCGCGC 1 cut(s) 795
RsaI GTAC 1 cut(s) 554
RsaNI GTAC 1 cut(s) 553
RseI CAYNNNNRTG 1 cut(s) 231
SaqAI TTAA 1 cut(s) 618
SatI GCNGC 5 cut(s) 549, 734, 737, 740, 743
Sau3AI GATC 4 cut(s) 67, 139, 163, 187
SchI GAGTC 1 cut(s) 386
ScrFI CCNGG 2 cut(s) 314, 414
SduI GDGCHC 1 cut(s) 455
SetI ASST 9 cut(s) 16, 182, 256, 277, 307, 679, 699, 712, 806
SfaNI GCATC 1 cut(s) 573
SfcI CTRYAG 1 cut(s) 740
SfuI TTCGAA 1 cut(s) 375
SmiMI CAYNNNNRTG 1 cut(s) 231
SphI GCATGC 1 cut(s) 372
Sse9I AATT 4 cut(s) 460, 620, 779, 895
SseBI AGGCCT 2 cut(s) 133, 529
SsiI CCGC 3 cut(s) 583, 737, 760
StuI AGGCCT 2 cut(s) 133, 529
StyD4I CCNGG 2 cut(s) 312, 412
StyI CCWWGG 2 cut(s) 128, 152
TaaI ACNGT 2 cut(s) 322, 835
TaiI ACGT 2 cut(s) 182, 699
TaqI TCGA 5 cut(s) 375, 516, 537, 699, 808
TasI AATT 4 cut(s) 460, 620, 779, 895
TauI GCSGC 1 cut(s) 739
TfiI GAWTC 2 cut(s) 589, 637
Tru1I TTAA 1 cut(s) 618
Tru9I TTAA 1 cut(s) 618
TscAI CASTG 1 cut(s) 455
TseFI GTSAC 2 cut(s) 596, 811
TseI GCWGC 4 cut(s) 548, 733, 739, 742
Tsp45I GTSAC 2 cut(s) 596, 811
TspDTI ATGAA 3 cut(s) 261, 666, 900
TspGWI ACGGA 1 cut(s) 498
TspRI CASTG 1 cut(s) 455
XapI RAATTY 2 cut(s) 620, 895
XceI RCATGY 2 cut(s) 230, 372
XmnI GAANNNNTTC 1 cut(s) 849
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.