RchiOBHm_Chr2g0140121

expansin-A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0017331
Physical Location & Seq
Forward (+)
57470005 .. 57473050
3046 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51060

Sequence Viewer

Length: 939 bp
ATGTCTTTGAGAGCTTTGTGTTGGTCGTTCCTTGTCTTCTTTTTCGTTGTCTATGTCAATGCTCATGATCATCATAAGCCAAAGCCTCATGATCAGCATAAGCCAAAGCCTCATGACCAGCAGAAGCCAGCAAGGCCTGATGGTCAGCAGAAGCCAAGGCCTGATGATCAGCAGAAGCCAACGTTTGATGATCAGCAGAAGCCAAAGTTGCAGTCAACTGAATGGAAACATGCTCATGCCACATTCTATGAAGGAGGTTCCGGAACATTTGGTGGAGCTTGTGGATACCATGATGTAGTTCAAGAAGGTTATGGCCTGGACACAGTAGCATTGAGCAATGCATTGTTCAACAAGGGGGAGATGTGTGGTGCATGCTTCGAAATCAAATGCGAGGACTCGCCTCAATGGTGCAAGCCTGGCCAGCCAAGTCTCATTGTTACAGCAACCAACCAGTGCCCTCCTAATTACAACCAACCGAGTGATAACGGAGGATGGTGCAACGAACCCCGTGAACATTTCGACATAGCAAGGCCTGTGTTCGAGACTATTGCTGCGTACAAGGCCGGCATCGTTCCAGTCTCATACCGCAGGTTTTGCTTAAGGCGTCCAAGCCTGAGGATTCCATGTGACCACAAGACTGGAGGCATTAAATTTACAATAACTGGGAATCCTTATTTCAATGAAGTGTTGGTGTGGAATGTTGGAGGTGCTGGGGATGTATGCAACGTCAAAGTGAAACCTGAAGACCAACCGAATACATGGCTGCCGCTGCAACGAATGTGGGGTCAGCGGTGGATTTACAACGGAAAATTGTGCGAGCAAGGCGCGCTTAGCTTTCGAGTGACATCGGGTGATGGAAGAACCGTAATCTCTGAAAATGTTCTCCCAAAGGGATGGCAGTTTGGCCAGACTTATGAAGGCAAGAATTTCGCCGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

312

Amino Acids

35.19

Weight (kDa)

8.39

Isoelectric Point (pI)

39.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXPB1_D1 PF22514 80 - 168 8.8e-08 EXPB1-like domain 1
DPBB_1 PF03330 108 - 195 4e-19 Lytic transglycolase
Expansin_C PF01357 216 - 296 8.2e-25 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017331)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g16911
rosa_chinensis RchiOBHm_Chr2g0140121 RchiOBHm_Chr3g0470941
rosa_laevigata RLG00000024231
rosa_multiflora Rmu_sc0005264.1_g000003
rosa_roxburghii Rroxscaffold_6G00410390
rosa_rugosa Rorug03G0114100
rosa_samantha Rh2AG411900 Rh2CG398000 Rh3BG189400 Rh3DG186800
rosa_wichuraiana Rw2G033750 Rw3G015420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 579
AccII CGCG 1 cut(s) 827
AccIII TCCGGA 1 cut(s) 260
AciI CCGC 3 cut(s) 586, 767, 790
AclI AACGTT 1 cut(s) 182
AcoI YGGCCR 2 cut(s) 418, 904
AcsI RAATTY 2 cut(s) 650, 925
AcuI CTGAAG 1 cut(s) 762
AcyI GRCGYC 1 cut(s) 604
AfaI GTAC 1 cut(s) 557
AflII CTTAAG 1 cut(s) 598
AgsI TTSAA 3 cut(s) 302, 349, 679
AjnI CCWGG 2 cut(s) 315, 415
AluBI AGCT 3 cut(s) 14, 278, 834
AluI AGCT 3 cut(s) 14, 278, 834
Alw26I GTCTC 3 cut(s) 434, 536, 583
Aor13HI TCCGGA 1 cut(s) 260
AoxI GGCC 7 cut(s) 134, 158, 313, 418, 530, 561, 904
ApeKI GCWGC 3 cut(s) 551, 763, 769
ApoI RAATTY 2 cut(s) 650, 925
ArsI GACNNNNNNTTYG 4 cut(s) 197, 229, 769, 801
Asp700I GAANNNNTTC 1 cut(s) 879
AspLEI GCGC 2 cut(s) 827, 829
AsuHPI GGTGA 1 cut(s) 863
AsuII TTCGAA 1 cut(s) 378
AxyI CCTNAGG 1 cut(s) 614
BaeGI GKGCMC 1 cut(s) 458
BalI TGGCCA 2 cut(s) 420, 906
BbsI GAAGAC 2 cut(s) 28, 750
BbvI GCAGC 3 cut(s) 538, 750, 756
BccI CCATC 4 cut(s) 134, 486, 848, 888
BceAI ACGGC 1 cut(s) 917
BcgI CGANNNNNNTGC 3 cut(s) 530, 564, 910
BciT130I CCWGG 2 cut(s) 317, 417
BciVI GTATCC 1 cut(s) 278
BclI TGATCA 4 cut(s) 67, 91, 166, 190
BcoDI GTCTC 3 cut(s) 434, 536, 583
BfrI CTTAAG 1 cut(s) 598
BfuAI ACCTGC 1 cut(s) 579
BfuI GTATCC 1 cut(s) 278
BglI GCCNNNNNGGC 1 cut(s) 133
BisI GCNGC 4 cut(s) 552, 764, 767, 770
BlpI GCTNAGC 1 cut(s) 830
BlsI GCNGC 4 cut(s) 553, 765, 768, 771
Bme1390I CCNGG 2 cut(s) 317, 417
BmiI GGNNCC 1 cut(s) 259
BmrFI CCNGG 2 cut(s) 317, 417
BmrI ACTGGG 1 cut(s) 672
BmsI GCATC 1 cut(s) 576
BmuI ACTGGG 1 cut(s) 672
BpiI GAAGAC 2 cut(s) 28, 750
BpmI CTGGAG 1 cut(s) 660
Bpu1102I GCTNAGC 1 cut(s) 830
Bpu14I TTCGAA 1 cut(s) 378
BsaHI GRCGYC 1 cut(s) 604
BsaJI CCNNGG 1 cut(s) 155
BsaWI WCCGGW 1 cut(s) 260
Bse118I RCCGGY 1 cut(s) 563
Bse1I ACTGG 4 cut(s) 451, 575, 643, 667
Bse21I CCTNAGG 1 cut(s) 614
Bse3DI GCAATG 1 cut(s) 343
BseAI TCCGGA 1 cut(s) 260
BseBI CCWGG 2 cut(s) 317, 417
BseDI CCNNGG 1 cut(s) 155
BseGI GGATG 3 cut(s) 497, 721, 899
BseMI GCAATG 1 cut(s) 343
BseMII CTCAG 1 cut(s) 605
BseNI ACTGG 4 cut(s) 451, 575, 643, 667
BsePI GCGCGC 1 cut(s) 825
BseSI GKGCMC 1 cut(s) 458
BseXI GCAGC 3 cut(s) 538, 750, 756
BseYI CCCAGC 1 cut(s) 710
Bsh1236I CGCG 1 cut(s) 827
BshFI GGCC 7 cut(s) 136, 160, 315, 420, 532, 563, 906
BsiSI CCGG 2 cut(s) 261, 564
BsmAI GTCTC 3 cut(s) 434, 536, 583
BsnI GGCC 7 cut(s) 136, 160, 315, 420, 532, 563, 906
Bsp119I TTCGAA 1 cut(s) 378
Bsp1286I GDGCHC 1 cut(s) 458
Bsp13I TCCGGA 1 cut(s) 260
Bsp143I GATC 4 cut(s) 67, 91, 166, 190
Bsp1720I GCTNAGC 1 cut(s) 830
BspACI CCGC 3 cut(s) 586, 767, 790
BspANI GGCC 7 cut(s) 136, 160, 315, 420, 532, 563, 906
BspCNI CTCAG 1 cut(s) 606
BspEI TCCGGA 1 cut(s) 260
BspFNI CGCG 1 cut(s) 827
BspHI TCATGA 3 cut(s) 64, 88, 112
BspLI GGNNCC 1 cut(s) 259
BspMI ACCTGC 1 cut(s) 579
BspT104I TTCGAA 1 cut(s) 378
BspTI CTTAAG 1 cut(s) 598
BsrDI GCAATG 1 cut(s) 343
BsrFI RCCGGY 1 cut(s) 563
BsrI ACTGG 4 cut(s) 451, 575, 643, 667
BssAI RCCGGY 1 cut(s) 563
BssECI CCNNGG 1 cut(s) 155
BssHII GCGCGC 1 cut(s) 825
BssMI GATC 4 cut(s) 67, 91, 166, 190
BssNI GRCGYC 1 cut(s) 604
BssT1I CCWWGG 1 cut(s) 155
Bst2UI CCWGG 2 cut(s) 317, 417
Bst4CI ACNGT 2 cut(s) 325, 865
BstACI GRCGYC 1 cut(s) 604
BstAFI CTTAAG 1 cut(s) 598
BstAPI GCANNNNNTGC 1 cut(s) 594
BstBI TTCGAA 1 cut(s) 378
BstC8I GCNNGC 7 cut(s) 129, 373, 413, 422, 565, 818, 827
BstDEI CTNAG 2 cut(s) 614, 830
BstF5I GGATG 3 cut(s) 497, 721, 899
BstFNI CGCG 1 cut(s) 827
BstHHI GCGC 2 cut(s) 827, 829
BstKTI GATC 4 cut(s) 70, 94, 169, 193
BstMAI GTCTC 3 cut(s) 434, 536, 583
BstMBI GATC 4 cut(s) 67, 91, 166, 190
BstNI CCWGG 2 cut(s) 317, 417
BstNSI RCATGY 2 cut(s) 233, 375
BstSCI CCNGG 2 cut(s) 315, 415
BstSLI GKGCMC 1 cut(s) 458
BstUI CGCG 1 cut(s) 827
BstV1I GCAGC 3 cut(s) 538, 750, 756
BstV2I GAAGAC 2 cut(s) 28, 750
BstXI CCANNNNNNTGG 2 cut(s) 638, 894
Bsu36I CCTNAGG 1 cut(s) 614
BsuI GTATCC 1 cut(s) 278
BsuRI GGCC 7 cut(s) 136, 160, 315, 420, 532, 563, 906
BtsCI GGATG 3 cut(s) 497, 721, 899
BtsIMutI CAGTG 1 cut(s) 458
BveI ACCTGC 1 cut(s) 579
Cac8I GCNNGC 7 cut(s) 129, 373, 413, 422, 565, 818, 827
CciI TCATGA 3 cut(s) 64, 88, 112
CfoI GCGC 2 cut(s) 827, 829
Cfr10I RCCGGY 1 cut(s) 563
CseI GACGC 1 cut(s) 593
Csp6I GTAC 1 cut(s) 556
CspCI CAANNNNNGTGG 2 cut(s) 761, 796
CviAII CATG 9 cut(s) 65, 89, 113, 230, 236, 290, 372, 624, 759
CviQI GTAC 1 cut(s) 556
DdeI CTNAG 2 cut(s) 614, 830
DpnI GATC 4 cut(s) 69, 93, 168, 192
DpnII GATC 4 cut(s) 67, 91, 166, 190
EaeI YGGCCR 2 cut(s) 418, 904
Eco130I CCWWGG 1 cut(s) 155
Eco147I AGGCCT 3 cut(s) 136, 160, 532
Eco57I CTGAAG 1 cut(s) 762
Eco81I CCTNAGG 1 cut(s) 614
EcoRII CCWGG 2 cut(s) 315, 415
EcoT14I CCWWGG 1 cut(s) 155
EcoT22I ATGCAT 1 cut(s) 343
ErhI CCWWGG 1 cut(s) 155
FaeI CATG 9 cut(s) 68, 92, 116, 233, 239, 293, 375, 627, 762
FalI AAGNNNNNCTT 2 cut(s) 813, 845
FatI CATG 9 cut(s) 64, 88, 112, 229, 235, 289, 371, 623, 758
FbaI TGATCA 4 cut(s) 67, 91, 166, 190
Fnu4HI GCNGC 4 cut(s) 552, 764, 767, 770
FokI GGATG 3 cut(s) 504, 728, 906
Fsp4HI GCNGC 4 cut(s) 552, 764, 767, 770
GlaI GCGC 2 cut(s) 826, 828
GluI GCNGC 4 cut(s) 552, 764, 767, 770
GsaI CCCAGC 1 cut(s) 714
GsuI CTGGAG 1 cut(s) 660
HaeIII GGCC 7 cut(s) 136, 160, 315, 420, 532, 563, 906
HapII CCGG 2 cut(s) 261, 564
HgaI GACGC 1 cut(s) 593
HhaI GCGC 2 cut(s) 827, 829
Hin1I GRCGYC 1 cut(s) 604
Hin1II CATG 9 cut(s) 68, 92, 116, 233, 239, 293, 375, 627, 762
Hin6I GCGC 2 cut(s) 825, 827
HinP1I GCGC 2 cut(s) 825, 827
HincII GTYRAC 1 cut(s) 216
HindII GTYRAC 1 cut(s) 216
HinfI GANTC 3 cut(s) 395, 619, 667
HpaII CCGG 2 cut(s) 261, 564
HphI GGTGA 1 cut(s) 863
Hpy166II GTNNAC 2 cut(s) 216, 512
Hpy188I TCNGA 1 cut(s) 874
Hpy188III TCNNGA 6 cut(s) 65, 89, 113, 261, 302, 541
Hpy8I GTNNAC 2 cut(s) 216, 512
HpyAV CCTTC 3 cut(s) 245, 299, 911
HpyCH4III ACNGT 2 cut(s) 325, 865
HpyCH4IV ACGT 2 cut(s) 182, 726
HpyCH4V TGCA 7 cut(s) 211, 341, 371, 411, 498, 723, 772
HpyF3I CTNAG 2 cut(s) 614, 830
HpySE526I ACGT 2 cut(s) 182, 726
Hsp92I GRCGYC 1 cut(s) 604
Hsp92II CATG 9 cut(s) 68, 92, 116, 233, 239, 293, 375, 627, 762
HspAI GCGC 2 cut(s) 825, 827
Kpn2I TCCGGA 1 cut(s) 260
KroI GCCGGC 1 cut(s) 563
KroNI GCCGGC 1 cut(s) 565
Ksp22I TGATCA 4 cut(s) 67, 91, 166, 190
Kzo9I GATC 4 cut(s) 67, 91, 166, 190
LmnI GCTCC 1 cut(s) 275
Lsp1109I GCAGC 3 cut(s) 538, 750, 756
LweI GCATC 1 cut(s) 576
MaeII ACGT 2 cut(s) 182, 726
MaeIII GTNAC 3 cut(s) 436, 626, 841
MalI GATC 4 cut(s) 69, 93, 168, 192
MboI GATC 4 cut(s) 67, 91, 166, 190
MboII GAAGA 3 cut(s) 28, 755, 870
MhlI GDGCHC 1 cut(s) 458
MlsI TGGCCA 2 cut(s) 420, 906
MluCI AATT 4 cut(s) 463, 650, 809, 925
MluNI TGGCCA 2 cut(s) 420, 906
MlyI GAGTC 1 cut(s) 389
MmeI TCCRAC 1 cut(s) 682
Mox20I TGGCCA 2 cut(s) 420, 906
Mph1103I ATGCAT 1 cut(s) 343
MroI TCCGGA 1 cut(s) 260
MroNI GCCGGC 1 cut(s) 563
MroXI GAANNNNTTC 1 cut(s) 879
MscI TGGCCA 2 cut(s) 420, 906
MseI TTAA 2 cut(s) 599, 648
MslI CAYNNNNRTG 1 cut(s) 234
Msp20I TGGCCA 2 cut(s) 420, 906
MspA1I CMGCKG 2 cut(s) 769, 790
MspCI CTTAAG 1 cut(s) 598
MspI CCGG 2 cut(s) 261, 564
MspR9I CCNGG 2 cut(s) 317, 417
MvaI CCWGG 2 cut(s) 317, 417
MvnI CGCG 1 cut(s) 827
NaeI GCCGGC 1 cut(s) 565
NdeII GATC 4 cut(s) 67, 91, 166, 190
NgoMIV GCCGGC 1 cut(s) 563
NlaIII CATG 9 cut(s) 68, 92, 116, 233, 239, 293, 375, 627, 762
NlaIV GGNNCC 1 cut(s) 259
NmuCI GTSAC 2 cut(s) 626, 841
NsiI ATGCAT 1 cut(s) 343
NspI RCATGY 2 cut(s) 233, 375
NspV TTCGAA 1 cut(s) 378
PaeI GCATGC 1 cut(s) 375
PagI TCATGA 3 cut(s) 64, 88, 112
PauI GCGCGC 1 cut(s) 825
PceI AGGCCT 3 cut(s) 136, 160, 532
PdiI GCCGGC 1 cut(s) 565
PdmI GAANNNNTTC 1 cut(s) 879
PfeI GAWTC 2 cut(s) 619, 667
PkrI GCNGC 4 cut(s) 553, 765, 768, 771
PleI GAGTC 1 cut(s) 389
PpsI GAGTC 1 cut(s) 389
Psp1406I AACGTT 1 cut(s) 182
Psp6I CCWGG 2 cut(s) 315, 415
PspFI CCCAGC 1 cut(s) 710
PspGI CCWGG 2 cut(s) 315, 415
PspN4I GGNNCC 1 cut(s) 259
PteI GCGCGC 1 cut(s) 825
RsaI GTAC 1 cut(s) 557
RsaNI GTAC 1 cut(s) 556
RseI CAYNNNNRTG 1 cut(s) 234
SaqAI TTAA 2 cut(s) 599, 648
SatI GCNGC 4 cut(s) 552, 764, 767, 770
Sau3AI GATC 4 cut(s) 67, 91, 166, 190
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 2 cut(s) 317, 417
SduI GDGCHC 1 cut(s) 458
SfaNI GCATC 1 cut(s) 576
SfuI TTCGAA 1 cut(s) 378
SmiMI CAYNNNNRTG 1 cut(s) 234
SmlI CTYRAG 1 cut(s) 598
SmoI CTYRAG 1 cut(s) 598
SphI GCATGC 1 cut(s) 375
Sse9I AATT 4 cut(s) 463, 650, 809, 925
SseBI AGGCCT 3 cut(s) 136, 160, 532
SsiI CCGC 3 cut(s) 586, 767, 790
StuI AGGCCT 3 cut(s) 136, 160, 532
StyD4I CCNGG 2 cut(s) 315, 415
StyI CCWWGG 1 cut(s) 155
TaaI ACNGT 2 cut(s) 325, 865
TaiI ACGT 2 cut(s) 185, 729
TaqI TCGA 4 cut(s) 378, 519, 540, 838
TasI AATT 4 cut(s) 463, 650, 809, 925
TauI GCSGC 1 cut(s) 769
TfiI GAWTC 2 cut(s) 619, 667
Tru1I TTAA 2 cut(s) 599, 648
Tru9I TTAA 2 cut(s) 599, 648
TscAI CASTG 1 cut(s) 458
TseFI GTSAC 2 cut(s) 626, 841
TseI GCWGC 3 cut(s) 551, 763, 769
Tsp45I GTSAC 2 cut(s) 626, 841
TspDTI ATGAA 3 cut(s) 264, 696, 930
TspGWI ACGGA 2 cut(s) 501, 819
TspRI CASTG 1 cut(s) 458
Vha464I CTTAAG 1 cut(s) 598
XapI RAATTY 2 cut(s) 650, 925
XceI RCATGY 2 cut(s) 233, 375
XmnI GAANNNNTTC 1 cut(s) 879
Zsp2I ATGCAT 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.