FvH4_6g19320

Alpha beta hydrolase domain-containing protein 11-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0014370
Physical Location & Seq
Reverse (-)
12858898 .. 12861435
2538 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g19320.t1

Sequence Viewer

Length: 969 bp
ATGGCTGGAACCCTACTGAACAAACCGGGTTTTCGCCACCTCCTGACCCGATTCACCACCTCACGGCGGTCTCTCCAAACCCTAGCCTACGAAGAAGTCCGAGCCACCACCGCCACGCCCTACCACTCCACCGCTTTCGTACTCCACGGCCTCCTCGGCTCCGCCAGAAATTGGAGATCTTTCTCTCGCACCCTCCTCTCCAATATCTCCGATCCTTCTGGGTGGAGATTGGTGCTTGTGGATTTGAGGAACCATGGGAAATCGGCTGGCCTCGAAGGCCTGAACCCGCCTCATGACTTGGTCAATGCAGCACAAGACTTGGCTGATTTGGTTAAGTCTGAGAATTGGGGTTGGCCTGATGTTGTTCTTGGTCACTCCATGGGTGGTAAGGTGGCTCTGCAGTTTGCTCAGAGCTGTGCTGCTGGCCACTATGGTGATCACGCTAAGTTGCCTAAACAGCTGTGGGTATTGGATTCTGTTCCAGGAGAAGTGAACCCTATACACAGTGATGGGGATGTTGAGAGAGTTTTGAAGACATTGCAGGGACTACCTTCAACAGTTCCATCACGAAAGTGGCTTGTGAATCATATGATAGAACTTGGGTTCTCAAAATCTTTGTCAGAATGGATAGGCACCAACCTCAAGAAAGTTGGAGATCATGAGACATGGGCATTCAATCTTGATGGTGCTGTTGAGATGTTCAAATCATACCGGGAGAAGTCATACGTGCCTCTGTTGGAGCATCCTCCAAAAGATATGGAAATAGCTATTGTGTGCGCTGAGAACAGTGATCGTTGGGACCCAGAGGTGATTCAGCAGCTTGAAAGCCTTGCTAGTCAGGAAAGAGATGGGTCGGAGGGGAAGGTTTCACTTCATGTTCTTCCCAAGTCAGGGCATTGGGTTCACGTGGATAATCCAAAAGGTCTTCTTGAGATTGTGGCACCAAAGATTTCATCCCTTTCGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_6

Protein Analysis

323

Amino Acids

35.62

Weight (kDa)

6.57

Isoelectric Point (pI)

37.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 46 - 140 3.9e-09 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 47 - 310 5.6e-19 Alpha/beta hydrolase family
Abhydrolase_1 PF00561 47 - 137 1.5e-11 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014370)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52570
fragaria_vesca FvH4_6g19320
malus_domestica MD12G1028900.v1.1
prunus_persica Prupe.7G102600_v2.0.a1 Prupe.7G102600_v2.0.a1
pyrus_communis pycom12g02770 pycom14g02720
rosa_chinensis RchiOBHm_Chr3g0474561
rosa_laevigata RLG00000023911
rosa_multiflora Rmu_sc0000535.1_g000018
rosa_roxburghii Rroxscaffold_6G00407070
rosa_rugosa Rorug03G0141500
rosa_samantha Rh3AG192200 Rh3BG222100 Rh3CG216800 Rh3DG217600
rosa_wichuraiana Rw3G017540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 632, 940
AccB7I CCANNNNNTGG 1 cut(s) 171
AciI CCGC 5 cut(s) 67, 111, 132, 162, 287
AclWI GGATC 1 cut(s) 206
AcoI YGGCCR 1 cut(s) 424
AcvI CACGTG 1 cut(s) 907
AfaI GTAC 1 cut(s) 141
AfiI CCNNNNNNNGG 5 cut(s) 63, 66, 171, 890, 891
AgsI TTSAA 5 cut(s) 532, 555, 676, 703, 824
AjnI CCWGG 1 cut(s) 481
AleI CACNNNNGTG 2 cut(s) 432, 571
AluBI AGCT 4 cut(s) 414, 460, 767, 820
AluI AGCT 4 cut(s) 414, 460, 767, 820
Alw26I GTCTC 2 cut(s) 75, 656
AlwI GGATC 1 cut(s) 206
AoxI GGCC 5 cut(s) 148, 268, 277, 353, 424
ApeKI GCWGC 3 cut(s) 308, 419, 817
ArsI GACNNNNNNTTYG 2 cut(s) 602, 634
AspLEI GCGC 1 cut(s) 779
AspS9I GGNCC 1 cut(s) 799
AsuC2I CCSGG 2 cut(s) 27, 713
AsuHPI GGTGA 3 cut(s) 46, 446, 820
AsuII TTCGAA 1 cut(s) 962
AvaII GGWCC 1 cut(s) 799
BalI TGGCCA 1 cut(s) 426
BanI GGYRCC 2 cut(s) 632, 940
BbrPI CACGTG 1 cut(s) 907
BbsI GAAGAC 2 cut(s) 539, 917
BbvI GCAGC 3 cut(s) 320, 406, 829
BccI CCATC 4 cut(s) 503, 571, 677, 842
BceAI ACGGC 2 cut(s) 80, 163
BciT130I CCWGG 1 cut(s) 483
BclI TGATCA 1 cut(s) 436
BcnI CCSGG 2 cut(s) 27, 713
BcoDI GTCTC 2 cut(s) 75, 656
BfaI CTAG 2 cut(s) 83, 834
BfmI CTRYAG 1 cut(s) 398
BglI GCCNNNNNGGC 2 cut(s) 156, 276
BglII AGATCT 1 cut(s) 176
BisI GCNGC 3 cut(s) 309, 420, 818
BlsI GCNGC 3 cut(s) 310, 421, 819
Bme1390I CCNGG 3 cut(s) 27, 483, 713
Bme18I GGWCC 1 cut(s) 799
BmgT120I GGNCC 1 cut(s) 799
BmiI GGNNCC 7 cut(s) 10, 160, 251, 634, 800, 801, 942
BmrFI CCNGG 3 cut(s) 27, 483, 713
BmsI GCATC 1 cut(s) 751
BpiI GAAGAC 2 cut(s) 539, 917
Bpu14I TTCGAA 1 cut(s) 962
BpuEI CTTGAG 2 cut(s) 626, 950
BpuMI CCSGG 2 cut(s) 27, 713
BsaAI YACGTR 2 cut(s) 727, 907
BsaI GGTCTC 1 cut(s) 75
BsaJI CCNNGG 4 cut(s) 145, 154, 253, 378
Bsc4I CCNNNNNNNGG 5 cut(s) 63, 66, 171, 890, 891
Bse3DI GCAATG 1 cut(s) 536
BseBI CCWGG 1 cut(s) 483
BseDI CCNNGG 4 cut(s) 145, 154, 253, 378
BseGI GGATG 3 cut(s) 520, 742, 953
BseLI CCNNNNNNNGG 5 cut(s) 63, 66, 171, 890, 891
BseMI GCAATG 1 cut(s) 536
BseMII CTCAG 3 cut(s) 330, 422, 771
BseRI GAGGAG 2 cut(s) 143, 185
BseXI GCAGC 3 cut(s) 320, 406, 829
BshFI GGCC 5 cut(s) 150, 270, 279, 355, 426
BshNI GGYRCC 2 cut(s) 632, 940
BsiSI CCGG 2 cut(s) 26, 712
BslFI GGGAC 2 cut(s) 558, 812
BslI CCNNNNNNNGG 5 cut(s) 63, 66, 171, 890, 891
BsmAI GTCTC 2 cut(s) 75, 656
BsmFI GGGAC 2 cut(s) 558, 812
BsmI GAATGC 1 cut(s) 671
BsnI GGCC 5 cut(s) 150, 270, 279, 355, 426
Bso31I GGTCTC 1 cut(s) 75
Bsp119I TTCGAA 1 cut(s) 962
Bsp143I GATC 5 cut(s) 176, 211, 436, 655, 790
Bsp19I CCATGG 2 cut(s) 253, 378
BspACI CCGC 5 cut(s) 67, 111, 132, 162, 287
BspANI GGCC 5 cut(s) 150, 270, 279, 355, 426
BspCNI CTCAG 3 cut(s) 331, 421, 772
BspHI TCATGA 2 cut(s) 292, 658
BspLI GGNNCC 7 cut(s) 10, 160, 251, 634, 800, 801, 942
BspMAI CTGCAG 1 cut(s) 402
BspPI GGATC 1 cut(s) 206
BspT104I TTCGAA 1 cut(s) 962
BspT107I GGYRCC 2 cut(s) 632, 940
BspTNI GGTCTC 1 cut(s) 75
BsrDI GCAATG 1 cut(s) 536
BssECI CCNNGG 4 cut(s) 145, 154, 253, 378
BssMI GATC 5 cut(s) 176, 211, 436, 655, 790
BssT1I CCWWGG 2 cut(s) 253, 378
Bst2UI CCWGG 1 cut(s) 483
Bst4CI ACNGT 3 cut(s) 506, 559, 788
BstBAI YACGTR 2 cut(s) 727, 907
BstBI TTCGAA 1 cut(s) 962
BstC8I GCNNGC 2 cut(s) 268, 424
BstDEI CTNAG 4 cut(s) 339, 408, 444, 780
BstDSI CCRYGG 3 cut(s) 145, 253, 378
BstF5I GGATG 3 cut(s) 520, 742, 953
BstHHI GCGC 1 cut(s) 779
BstKTI GATC 5 cut(s) 179, 214, 439, 658, 793
BstMAI GTCTC 2 cut(s) 75, 656
BstMBI GATC 5 cut(s) 176, 211, 436, 655, 790
BstMWI GCNNNNNNNGC 4 cut(s) 110, 156, 276, 457
BstNI CCWGG 1 cut(s) 483
BstSCI CCNGG 3 cut(s) 25, 481, 711
BstSFI CTRYAG 1 cut(s) 398
BstV1I GCAGC 3 cut(s) 320, 406, 829
BstV2I GAAGAC 2 cut(s) 539, 917
BstX2I RGATCY 1 cut(s) 176
BstYI RGATCY 1 cut(s) 176
BsuRI GGCC 5 cut(s) 150, 270, 279, 355, 426
BtgI CCRYGG 3 cut(s) 145, 253, 378
BtsCI GGATG 3 cut(s) 520, 742, 953
BtsIMutI CAGTG 2 cut(s) 511, 793
Cac8I GCNNGC 2 cut(s) 268, 424
CciI TCATGA 2 cut(s) 292, 658
CfoI GCGC 1 cut(s) 779
Cfr13I GGNCC 1 cut(s) 799
Csp6I GTAC 1 cut(s) 140
CviAII CATG 6 cut(s) 254, 293, 379, 659, 666, 875
CviQI GTAC 1 cut(s) 140
DdeI CTNAG 4 cut(s) 339, 408, 444, 780
DpnI GATC 5 cut(s) 178, 213, 438, 657, 792
DpnII GATC 5 cut(s) 176, 211, 436, 655, 790
EaeI YGGCCR 1 cut(s) 424
EciI GGCGGA 1 cut(s) 151
Eco130I CCWWGG 2 cut(s) 253, 378
Eco147I AGGCCT 1 cut(s) 279
Eco31I GGTCTC 1 cut(s) 75
Eco47I GGWCC 1 cut(s) 799
Eco72I CACGTG 1 cut(s) 907
EcoO109I RGGNCCY 1 cut(s) 799
EcoRII CCWGG 1 cut(s) 481
EcoT14I CCWWGG 2 cut(s) 253, 378
ErhI CCWWGG 2 cut(s) 253, 378
FaeI CATG 6 cut(s) 257, 296, 382, 662, 669, 878
FalI AAGNNNNNCTT 2 cut(s) 912, 944
FaqI GGGAC 2 cut(s) 558, 812
FatI CATG 6 cut(s) 253, 292, 378, 658, 665, 874
FauI CCCGC 1 cut(s) 294
FauNDI CATATG 1 cut(s) 588
FbaI TGATCA 1 cut(s) 436
Fnu4HI GCNGC 3 cut(s) 309, 420, 818
FokI GGATG 3 cut(s) 527, 729, 940
Fsp4HI GCNGC 3 cut(s) 309, 420, 818
FspBI CTAG 2 cut(s) 83, 834
GlaI GCGC 1 cut(s) 778
GluI GCNGC 3 cut(s) 309, 420, 818
HaeIII GGCC 5 cut(s) 150, 270, 279, 355, 426
HapII CCGG 2 cut(s) 26, 712
HhaI GCGC 1 cut(s) 779
Hin1II CATG 6 cut(s) 257, 296, 382, 662, 669, 878
Hin6I GCGC 1 cut(s) 777
HinP1I GCGC 1 cut(s) 777
HinfI GANTC 4 cut(s) 51, 473, 583, 811
HpaII CCGG 2 cut(s) 26, 712
HphI GGTGA 3 cut(s) 46, 446, 820
Hpy166II GTNNAC 2 cut(s) 493, 904
Hpy188I TCNGA 6 cut(s) 101, 211, 340, 411, 622, 856
Hpy188III TCNNGA 8 cut(s) 43, 293, 567, 643, 659, 680, 839, 929
Hpy8I GTNNAC 2 cut(s) 493, 904
HpyAV CCTTC 4 cut(s) 225, 269, 561, 856
HpyCH4III ACNGT 3 cut(s) 506, 559, 788
HpyCH4IV ACGT 2 cut(s) 726, 906
HpyCH4V TGCA 3 cut(s) 308, 400, 541
HpyF10VI GCNNNNNNNGC 4 cut(s) 110, 156, 276, 457
HpyF3I CTNAG 4 cut(s) 339, 408, 444, 780
HpySE526I ACGT 2 cut(s) 726, 906
Hsp92II CATG 6 cut(s) 257, 296, 382, 662, 669, 878
HspAI GCGC 1 cut(s) 777
KflI GGGWCCC 1 cut(s) 799
Ksp22I TGATCA 1 cut(s) 436
Kzo9I GATC 5 cut(s) 176, 211, 436, 655, 790
LmnI GCTCC 2 cut(s) 164, 739
Lsp1109I GCAGC 3 cut(s) 320, 406, 829
LweI GCATC 1 cut(s) 751
MaeI CTAG 2 cut(s) 83, 834
MaeII ACGT 2 cut(s) 726, 906
MaeIII GTNAC 1 cut(s) 371
MalI GATC 5 cut(s) 178, 213, 438, 657, 792
MboI GATC 5 cut(s) 176, 211, 436, 655, 790
MboII GAAGA 4 cut(s) 104, 544, 872, 917
MflI RGATCY 1 cut(s) 176
MlsI TGGCCA 1 cut(s) 426
MluCI AATT 3 cut(s) 169, 343, 964
MluNI TGGCCA 1 cut(s) 426
MmeI TCCRAC 3 cut(s) 631, 717, 834
Mox20I TGGCCA 1 cut(s) 426
MscI TGGCCA 1 cut(s) 426
MseI TTAA 1 cut(s) 333
MslI CAYNNNNRTG 3 cut(s) 432, 507, 571
Msp20I TGGCCA 1 cut(s) 426
MspA1I CMGCKG 1 cut(s) 460
MspI CCGG 2 cut(s) 26, 712
MspR9I CCNGG 3 cut(s) 27, 483, 713
Mva1269I GAATGC 1 cut(s) 671
MvaI CCWGG 1 cut(s) 483
MwoI GCNNNNNNNGC 4 cut(s) 110, 156, 276, 457
NciI CCSGG 2 cut(s) 27, 713
NcoI CCATGG 2 cut(s) 253, 378
NdeI CATATG 1 cut(s) 588
NdeII GATC 5 cut(s) 176, 211, 436, 655, 790
NlaIII CATG 6 cut(s) 257, 296, 382, 662, 669, 878
NlaIV GGNNCC 7 cut(s) 10, 160, 251, 634, 800, 801, 942
NmeAIII GCCGAG 1 cut(s) 135
NmuCI GTSAC 1 cut(s) 371
NspV TTCGAA 1 cut(s) 962
OliI CACNNNNGTG 2 cut(s) 432, 571
PagI TCATGA 2 cut(s) 292, 658
PceI AGGCCT 1 cut(s) 279
PctI GAATGC 1 cut(s) 671
PfeI GAWTC 4 cut(s) 51, 473, 583, 811
PflFI GACNNNGTC 1 cut(s) 299
PflMI CCANNNNNTGG 1 cut(s) 171
PfoI TCCNGGA 1 cut(s) 481
PkrI GCNGC 3 cut(s) 310, 421, 819
PmaCI CACGTG 1 cut(s) 907
PmlI CACGTG 1 cut(s) 907
Ppu21I YACGTR 2 cut(s) 727, 907
PpuMI RGGWCCY 1 cut(s) 799
Psp5II RGGWCCY 1 cut(s) 799
Psp6I CCWGG 1 cut(s) 481
PspCI CACGTG 1 cut(s) 907
PspGI CCWGG 1 cut(s) 481
PspN4I GGNNCC 7 cut(s) 10, 160, 251, 634, 800, 801, 942
PspPI GGNCC 1 cut(s) 799
PspPPI RGGWCCY 1 cut(s) 799
PstI CTGCAG 1 cut(s) 402
PsuI RGATCY 1 cut(s) 176
PsyI GACNNNGTC 1 cut(s) 299
PvuII CAGCTG 1 cut(s) 460
RsaI GTAC 1 cut(s) 141
RsaNI GTAC 1 cut(s) 140
RseI CAYNNNNRTG 3 cut(s) 432, 507, 571
SaqAI TTAA 1 cut(s) 333
SatI GCNGC 3 cut(s) 309, 420, 818
Sau3AI GATC 5 cut(s) 176, 211, 436, 655, 790
Sau96I GGNCC 1 cut(s) 799
ScrFI CCNGG 3 cut(s) 27, 483, 713
SfaNI GCATC 1 cut(s) 751
SfcI CTRYAG 1 cut(s) 398
SfiI GGCCNNNNNGGCC 1 cut(s) 276
SfuI TTCGAA 1 cut(s) 962
SinI GGWCC 1 cut(s) 799
SmiMI CAYNNNNRTG 3 cut(s) 432, 507, 571
SmlI CTYRAG 2 cut(s) 641, 929
SmoI CTYRAG 2 cut(s) 641, 929
Sse9I AATT 3 cut(s) 169, 343, 964
SseBI AGGCCT 1 cut(s) 279
SsiI CCGC 5 cut(s) 67, 111, 132, 162, 287
SspMI CTAG 2 cut(s) 83, 834
StuI AGGCCT 1 cut(s) 279
StyD4I CCNGG 3 cut(s) 25, 481, 711
StyI CCWWGG 2 cut(s) 253, 378
TaaI ACNGT 3 cut(s) 506, 559, 788
TaiI ACGT 2 cut(s) 729, 909
TaqI TCGA 2 cut(s) 273, 962
TasI AATT 3 cut(s) 169, 343, 964
TfiI GAWTC 4 cut(s) 51, 473, 583, 811
Tru1I TTAA 1 cut(s) 333
Tru9I TTAA 1 cut(s) 333
TscAI CASTG 2 cut(s) 511, 793
TseFI GTSAC 1 cut(s) 371
TseI GCWGC 3 cut(s) 308, 419, 817
Tsp45I GTSAC 1 cut(s) 371
TspDTI ATGAA 2 cut(s) 863, 942
TspRI CASTG 2 cut(s) 511, 793
Tth111I GACNNNGTC 1 cut(s) 299
Van91I CCANNNNNTGG 1 cut(s) 171
VpaK11BI GGWCC 1 cut(s) 799
XcmI CCANNNNNNNNNTGG 1 cut(s) 570
XspI CTAG 2 cut(s) 83, 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.