MD12G1028900.v1.1

Alpha beta hydrolase domain-containing protein 11-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0014370
Physical Location & Seq
Reverse (-)
3209528 .. 3212442
2915 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1028900.v1.1.491

Sequence Viewer

Length: 972 bp
ATGGCTCGGATCCTCGGAAACAAACCGGGTCTTCACCACCTCCTGACCCGATTCACAATCTCACGACGGCCGCTGCAAACCCTGGCCTATGAAGAGCTCCGACCCTCCGACGCGGACCCACAACAACCCTACCGCTCCACCGCTTTCATCCTCCACGGCCTCCTGGGCTCCGCCAGAAACTGGCGAACTTTCTCTCGCTCCCTCCTCTCCCATCTCTCTGACCCATCTGGTTGGAGATTGGTGCTTGTGGATTTGAGGAACCATGGGAAATCGGCCGAGATCGAAGGTCTGAACCCACCTCATGATTTGTTCAATGGGGCCCGAGATTTGGCTGATTTGGTTGGGACTCAGGGTTGGGACTGGCCCGATGTTGTTCTCGGTCACTCCATGGGCGGTAAGGTTGCTCTGCAATTTGCAGAGAGCTGTGCCCTTGGTGACTACGGCGATTCCGCTAAGCTACCCAAGCAGCTGTGGGTATTGGATTCTGTTCCAGGAGAAGTGAGTCGTGACAAAAGTGATGGGGAAGTTGAGAAAGTTTTGCAGACATTGCAGAGTCTACCTTCATCAGTTCCATCTCGAAAGTGGCTTGTGAATCATATGATTGAACTTGGGTTCTCAAAGTCATTGTCAGAATGGATAGGCACCAACCTCAAGAAACAGGGAGATCACGAGACATGGGCGTTCAATCTTGATGGTGCTGTCCAGATGTTCAAGTCTTACCAGGAGAAGTCTTATTGGCCTCTGTTGGAGCACCCACCAAAAGAAATGGCAATAGATGTTGTGCGTGCTGAGAACAGTGATCGTTGGGACCCCGATGTGGTTCAACGGCTTGGAAGCCTTGCCAATAGGGAAGCCAATGGATCAGAGGGAAAGGTTTCAGTTCACGTTCTTCCCAAGTCTGGCCACTGGGTTCACGTGGACAATCCAAAAGGTCTTCTTGAGATTGTGGCACCAAGGATTTCATCCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_6

Protein Analysis

324

Amino Acids

36.02

Weight (kDa)

6.5

Isoelectric Point (pI)

38.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 45 - 140 1.7e-06 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 50 - 313 1.6e-18 Alpha/beta hydrolase family
Abhydrolase_1 PF00561 50 - 140 3.9e-11 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014370)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52570
fragaria_vesca FvH4_6g19320
malus_domestica MD12G1028900.v1.1
prunus_persica Prupe.7G102600_v2.0.a1 Prupe.7G102600_v2.0.a1
pyrus_communis pycom12g02770 pycom14g02720
rosa_chinensis RchiOBHm_Chr3g0474561
rosa_laevigata RLG00000023911
rosa_multiflora Rmu_sc0000535.1_g000018
rosa_roxburghii Rroxscaffold_6G00407070
rosa_rugosa Rorug03G0141500
rosa_samantha Rh3AG192200 Rh3BG222100 Rh3CG216800 Rh3DG217600
rosa_wichuraiana Rw3G017540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 641, 949
AccB7I CCANNNNNTGG 1 cut(s) 180
AccBSI CCGCTC 1 cut(s) 135
AccI GTMKAC 1 cut(s) 556
AccII CGCG 1 cut(s) 113
AciI CCGC 7 cut(s) 71, 113, 133, 141, 171, 393, 450
AclWI GGATC 3 cut(s) 4, 17, 868
AcoI YGGCCR 3 cut(s) 68, 273, 901
AcvI CACGTG 1 cut(s) 916
AfiI CCNNNNNNNGG 4 cut(s) 180, 328, 817, 899
AgsI TTSAA 5 cut(s) 313, 605, 685, 712, 824
AjnI CCWGG 4 cut(s) 81, 162, 490, 720
AluBI AGCT 4 cut(s) 97, 423, 457, 469
AluI AGCT 4 cut(s) 97, 423, 457, 469
Alw21I GWGCWC 2 cut(s) 99, 753
Alw26I GTCTC 1 cut(s) 665
AlwI GGATC 3 cut(s) 4, 17, 868
AlwNI CAGNNNCTG 1 cut(s) 180
Ama87I CYCGRG 1 cut(s) 321
AoxI GGCC 8 cut(s) 68, 84, 157, 273, 318, 362, 737, 901
ApaI GGGCCC 1 cut(s) 322
ApeKI GCWGC 2 cut(s) 73, 466
ArsI GACNNNNNNTTYG 2 cut(s) 611, 643
Asp700I GAANNNNTTC 1 cut(s) 874
AspS9I GGNCC 5 cut(s) 115, 318, 319, 363, 808
AsuC2I CCSGG 1 cut(s) 27
AsuHPI GGTGA 2 cut(s) 26, 446
AvaI CYCGRG 1 cut(s) 321
AvaII GGWCC 2 cut(s) 115, 808
BaeGI GKGCMC 2 cut(s) 322, 430
BalI TGGCCA 1 cut(s) 903
BamHI GGATCC 1 cut(s) 9
BanI GGYRCC 2 cut(s) 641, 949
BanII GRGCYC 3 cut(s) 99, 170, 322
BauI CACGAG 1 cut(s) 668
BbrPI CACGTG 1 cut(s) 916
BbsI GAAGAC 2 cut(s) 23, 926
Bbv12I GWGCWC 2 cut(s) 99, 753
BbvI GCAGC 2 cut(s) 60, 478
BccI CCATC 5 cut(s) 219, 232, 512, 580, 686
BceAI ACGGC 4 cut(s) 83, 172, 457, 842
BciT130I CCWGG 4 cut(s) 83, 164, 492, 722
BcnI CCSGG 1 cut(s) 27
BcoDI GTCTC 1 cut(s) 665
BglI GCCNNNNNGGC 1 cut(s) 165
BisI GCNGC 3 cut(s) 71, 74, 467
BlpI GCTNAGC 1 cut(s) 453
BlsI GCNGC 3 cut(s) 72, 75, 468
Bme1390I CCNGG 5 cut(s) 27, 83, 164, 492, 722
Bme18I GGWCC 2 cut(s) 115, 808
BmeT110I CYCGRG 1 cut(s) 321
BmgT120I GGNCC 5 cut(s) 115, 318, 319, 363, 808
BmrFI CCNGG 5 cut(s) 27, 83, 164, 492, 722
BmrI ACTGGG 1 cut(s) 916
BmuI ACTGGG 1 cut(s) 916
BpiI GAAGAC 2 cut(s) 23, 926
Bpu1102I GCTNAGC 1 cut(s) 453
BpuEI CTTGAG 2 cut(s) 635, 959
BpuMI CCSGG 1 cut(s) 27
BsaAI YACGTR 1 cut(s) 916
BsaJI CCNNGG 8 cut(s) 13, 81, 154, 163, 262, 387, 430, 953
Bsc4I CCNNNNNNNGG 4 cut(s) 180, 328, 817, 899
Bse1I ACTGG 3 cut(s) 185, 365, 911
Bse3DI GCAATG 1 cut(s) 545
BseBI CCWGG 4 cut(s) 83, 164, 492, 722
BseDI CCNNGG 8 cut(s) 13, 81, 154, 163, 262, 387, 430, 953
BseGI GGATG 2 cut(s) 147, 962
BseLI CCNNNNNNNGG 4 cut(s) 180, 328, 817, 899
BseMI GCAATG 1 cut(s) 545
BseMII CTCAG 2 cut(s) 362, 780
BseNI ACTGG 3 cut(s) 185, 365, 911
BseRI GAGGAG 1 cut(s) 194
BseSI GKGCMC 2 cut(s) 322, 430
BseX3I CGGCCG 2 cut(s) 68, 273
BseXI GCAGC 2 cut(s) 60, 478
Bsh1236I CGCG 1 cut(s) 113
Bsh1285I CGRYCG 2 cut(s) 71, 276
BshFI GGCC 8 cut(s) 70, 86, 159, 275, 320, 364, 739, 903
BshNI GGYRCC 2 cut(s) 641, 949
BsiEI CGRYCG 2 cut(s) 71, 276
BsiHKAI GWGCWC 2 cut(s) 99, 753
BsiHKCI CYCGRG 1 cut(s) 321
BsiSI CCGG 1 cut(s) 26
BslFI GGGAC 3 cut(s) 358, 371, 821
BslI CCNNNNNNNGG 4 cut(s) 180, 328, 817, 899
BsmAI GTCTC 1 cut(s) 665
BsmFI GGGAC 3 cut(s) 358, 371, 821
BsnI GGCC 8 cut(s) 70, 86, 159, 275, 320, 364, 739, 903
BsoBI CYCGRG 1 cut(s) 321
Bsp120I GGGCCC 1 cut(s) 318
Bsp1286I GDGCHC 5 cut(s) 99, 170, 322, 430, 753
Bsp143I GATC 5 cut(s) 9, 279, 664, 799, 860
Bsp1720I GCTNAGC 1 cut(s) 453
Bsp19I CCATGG 2 cut(s) 262, 387
BspACI CCGC 7 cut(s) 71, 113, 133, 141, 171, 393, 450
BspANI GGCC 8 cut(s) 70, 86, 159, 275, 320, 364, 739, 903
BspCNI CTCAG 2 cut(s) 361, 781
BspFNI CGCG 1 cut(s) 113
BspHI TCATGA 1 cut(s) 301
BspPI GGATC 3 cut(s) 4, 17, 868
BspQI GCTCTTC 1 cut(s) 87
BspT107I GGYRCC 2 cut(s) 641, 949
BsrBI CCGCTC 1 cut(s) 135
BsrDI GCAATG 1 cut(s) 545
BsrI ACTGG 3 cut(s) 185, 365, 911
BssECI CCNNGG 8 cut(s) 13, 81, 154, 163, 262, 387, 430, 953
BssMI GATC 5 cut(s) 9, 279, 664, 799, 860
BssSI CACGAG 1 cut(s) 668
BssT1I CCWWGG 4 cut(s) 262, 387, 430, 953
Bst2BI CACGAG 1 cut(s) 668
Bst2UI CCWGG 4 cut(s) 83, 164, 492, 722
Bst4CI ACNGT 1 cut(s) 797
Bst6I CTCTTC 1 cut(s) 87
BstAPI GCANNNNNTGC 1 cut(s) 547
BstBAI YACGTR 1 cut(s) 916
BstC8I GCNNGC 1 cut(s) 786
BstDEI CTNAG 3 cut(s) 348, 453, 789
BstDSI CCRYGG 3 cut(s) 154, 262, 387
BstF5I GGATG 2 cut(s) 147, 962
BstFNI CGCG 1 cut(s) 113
BstKTI GATC 5 cut(s) 12, 282, 667, 802, 863
BstMAI GTCTC 1 cut(s) 665
BstMBI GATC 5 cut(s) 9, 279, 664, 799, 860
BstMCI CGRYCG 2 cut(s) 71, 276
BstMWI GCNNNNNNNGC 3 cut(s) 165, 463, 547
BstNI CCWGG 4 cut(s) 83, 164, 492, 722
BstSCI CCNGG 5 cut(s) 25, 81, 162, 490, 720
BstSLI GKGCMC 2 cut(s) 322, 430
BstUI CGCG 1 cut(s) 113
BstV1I GCAGC 2 cut(s) 60, 478
BstV2I GAAGAC 2 cut(s) 23, 926
BstX2I RGATCY 1 cut(s) 9
BstXI CCANNNNNNTGG 1 cut(s) 231
BstYI RGATCY 1 cut(s) 9
BstZI CGGCCG 2 cut(s) 68, 273
BsuRI GGCC 8 cut(s) 70, 86, 159, 275, 320, 364, 739, 903
BtgI CCRYGG 3 cut(s) 154, 262, 387
BtsCI GGATG 2 cut(s) 147, 962
BtsIMutI CAGTG 2 cut(s) 802, 904
Cac8I GCNNGC 1 cut(s) 786
CaiI CAGNNNCTG 1 cut(s) 180
CciI TCATGA 1 cut(s) 301
Cfr13I GGNCC 5 cut(s) 115, 318, 319, 363, 808
CseI GACGC 1 cut(s) 119
CviAII CATG 4 cut(s) 263, 302, 388, 675
DdeI CTNAG 3 cut(s) 348, 453, 789
DpnI GATC 5 cut(s) 11, 281, 666, 801, 862
DpnII GATC 5 cut(s) 9, 279, 664, 799, 860
EaeI YGGCCR 3 cut(s) 68, 273, 901
EagI CGGCCG 2 cut(s) 68, 273
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
EciI GGCGGA 1 cut(s) 160
Ecl136II GAGCTC 1 cut(s) 97
EclXI CGGCCG 2 cut(s) 68, 273
Eco130I CCWWGG 4 cut(s) 262, 387, 430, 953
Eco24I GRGCYC 3 cut(s) 99, 170, 322
Eco47I GGWCC 2 cut(s) 115, 808
Eco52I CGGCCG 2 cut(s) 68, 273
Eco53kI GAGCTC 1 cut(s) 97
Eco72I CACGTG 1 cut(s) 916
Eco88I CYCGRG 1 cut(s) 321
EcoICRI GAGCTC 1 cut(s) 97
EcoO109I RGGNCCY 2 cut(s) 318, 808
EcoRII CCWGG 4 cut(s) 81, 162, 490, 720
EcoT14I CCWWGG 4 cut(s) 262, 387, 430, 953
EcoT38I GRGCYC 3 cut(s) 99, 170, 322
ErhI CCWWGG 4 cut(s) 262, 387, 430, 953
FaeI CATG 4 cut(s) 266, 305, 391, 678
FaiI YATR 7 cut(s) 90, 264, 303, 389, 597, 599, 676
FalI AAGNNNNNCTT 2 cut(s) 921, 953
FaqI GGGAC 3 cut(s) 358, 371, 821
FatI CATG 4 cut(s) 262, 301, 387, 674
FauNDI CATATG 1 cut(s) 597
FblI GTMKAC 1 cut(s) 556
Fnu4HI GCNGC 3 cut(s) 71, 74, 467
FokI GGATG 2 cut(s) 134, 949
FriOI GRGCYC 3 cut(s) 99, 170, 322
Fsp4HI GCNGC 3 cut(s) 71, 74, 467
GluI GCNGC 3 cut(s) 71, 74, 467
HaeIII GGCC 8 cut(s) 70, 86, 159, 275, 320, 364, 739, 903
HapII CCGG 1 cut(s) 26
HgaI GACGC 1 cut(s) 119
Hin1II CATG 4 cut(s) 266, 305, 391, 678
HinfI GANTC 7 cut(s) 51, 346, 446, 482, 502, 553, 592
HpaII CCGG 1 cut(s) 26
HphI GGTGA 2 cut(s) 26, 446
Hpy166II GTNNAC 4 cut(s) 557, 883, 913, 919
Hpy188I TCNGA 8 cut(s) 9, 17, 101, 109, 220, 291, 631, 865
Hpy8I GTNNAC 4 cut(s) 557, 883, 913, 919
Hpy99I CGWCG 2 cut(s) 69, 113
HpyAV CCTTC 2 cut(s) 278, 570
HpyCH4III ACNGT 1 cut(s) 797
HpyCH4IV ACGT 2 cut(s) 885, 915
HpyCH4V TGCA 5 cut(s) 76, 409, 416, 541, 550
HpyF10VI GCNNNNNNNGC 3 cut(s) 165, 463, 547
HpyF3I CTNAG 3 cut(s) 348, 453, 789
HpySE526I ACGT 2 cut(s) 885, 915
Hsp92II CATG 4 cut(s) 266, 305, 391, 678
KflI GGGWCCC 1 cut(s) 808
Kzo9I GATC 5 cut(s) 9, 279, 664, 799, 860
LguI GCTCTTC 1 cut(s) 87
LmnI GCTCC 5 cut(s) 102, 140, 173, 203, 748
Lsp1109I GCAGC 2 cut(s) 60, 478
MaeII ACGT 2 cut(s) 885, 915
MaeIII GTNAC 3 cut(s) 380, 434, 506
MalI GATC 5 cut(s) 11, 281, 666, 801, 862
MbiI CCGCTC 1 cut(s) 135
MboI GATC 5 cut(s) 9, 279, 664, 799, 860
MboII GAAGA 4 cut(s) 23, 104, 881, 926
MflI RGATCY 1 cut(s) 9
MhlI GDGCHC 5 cut(s) 99, 170, 322, 430, 753
MlsI TGGCCA 1 cut(s) 903
MluCI AATT 1 cut(s) 410
MluNI TGGCCA 1 cut(s) 903
MlyI GAGTC 3 cut(s) 340, 511, 562
MmeI TCCRAC 4 cut(s) 124, 132, 212, 726
Mox20I TGGCCA 1 cut(s) 903
MroXI GAANNNNTTC 1 cut(s) 874
MscI TGGCCA 1 cut(s) 903
Msp20I TGGCCA 1 cut(s) 903
MspA1I CMGCKG 2 cut(s) 73, 469
MspI CCGG 1 cut(s) 26
MspR9I CCNGG 5 cut(s) 27, 83, 164, 492, 722
MvaI CCWGG 4 cut(s) 83, 164, 492, 722
MvnI CGCG 1 cut(s) 113
MwoI GCNNNNNNNGC 3 cut(s) 165, 463, 547
NciI CCSGG 1 cut(s) 27
NcoI CCATGG 2 cut(s) 262, 387
NdeI CATATG 1 cut(s) 597
NdeII GATC 5 cut(s) 9, 279, 664, 799, 860
NlaIII CATG 4 cut(s) 266, 305, 391, 678
NmeAIII GCCGAG 1 cut(s) 301
NmuCI GTSAC 3 cut(s) 380, 434, 506
PagI TCATGA 1 cut(s) 301
PciSI GCTCTTC 1 cut(s) 87
PdmI GAANNNNTTC 1 cut(s) 874
PfeI GAWTC 4 cut(s) 51, 446, 482, 592
PflMI CCANNNNNTGG 1 cut(s) 180
PfoI TCCNGGA 1 cut(s) 490
PkrI GCNGC 3 cut(s) 72, 75, 468
PleI GAGTC 3 cut(s) 340, 510, 561
PmaCI CACGTG 1 cut(s) 916
PmlI CACGTG 1 cut(s) 916
PpsI GAGTC 3 cut(s) 340, 510, 561
Ppu21I YACGTR 1 cut(s) 916
PpuMI RGGWCCY 1 cut(s) 808
Psp124BI GAGCTC 1 cut(s) 99
Psp5II RGGWCCY 1 cut(s) 808
Psp6I CCWGG 4 cut(s) 81, 162, 490, 720
PspCI CACGTG 1 cut(s) 916
PspGI CCWGG 4 cut(s) 81, 162, 490, 720
PspOMI GGGCCC 1 cut(s) 318
PspPI GGNCC 5 cut(s) 115, 318, 319, 363, 808
PspPPI RGGWCCY 1 cut(s) 808
PstNI CAGNNNCTG 1 cut(s) 180
PsuI RGATCY 1 cut(s) 9
PvuII CAGCTG 1 cut(s) 469
SacI GAGCTC 1 cut(s) 99
SapI GCTCTTC 1 cut(s) 87
SatI GCNGC 3 cut(s) 71, 74, 467
Sau3AI GATC 5 cut(s) 9, 279, 664, 799, 860
Sau96I GGNCC 5 cut(s) 115, 318, 319, 363, 808
SchI GAGTC 3 cut(s) 340, 511, 562
ScrFI CCNGG 5 cut(s) 27, 83, 164, 492, 722
SduI GDGCHC 5 cut(s) 99, 170, 322, 430, 753
SinI GGWCC 2 cut(s) 115, 808
SmlI CTYRAG 2 cut(s) 650, 938
SmoI CTYRAG 2 cut(s) 650, 938
Sse9I AATT 1 cut(s) 410
SsiI CCGC 7 cut(s) 71, 113, 133, 141, 171, 393, 450
SstI GAGCTC 1 cut(s) 99
StyD4I CCNGG 5 cut(s) 25, 81, 162, 490, 720
StyI CCWWGG 4 cut(s) 262, 387, 430, 953
TaaI ACNGT 1 cut(s) 797
TaiI ACGT 2 cut(s) 888, 918
TaqI TCGA 2 cut(s) 282, 577
TaqII GACCGA 1 cut(s) 368
TasI AATT 1 cut(s) 410
TauI GCSGC 1 cut(s) 73
TfiI GAWTC 4 cut(s) 51, 446, 482, 592
TscAI CASTG 2 cut(s) 802, 911
TseFI GTSAC 3 cut(s) 380, 434, 506
TseI GCWGC 2 cut(s) 73, 466
Tsp45I GTSAC 3 cut(s) 380, 434, 506
TspDTI ATGAA 4 cut(s) 105, 136, 552, 951
TspRI CASTG 2 cut(s) 802, 911
Van91I CCANNNNNTGG 1 cut(s) 180
VpaK11BI GGWCC 2 cut(s) 115, 808
XcmI CCANNNNNNNNNTGG 1 cut(s) 579
XmiI GTMKAC 1 cut(s) 556
XmnI GAANNNNTTC 1 cut(s) 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.