FvH4_6g24840

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0017693
Physical Location & Seq
Forward (+)
18824666 .. 18826146
1481 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g24840.t1

Sequence Viewer

Length: 735 bp
ATGTGCATGCAGTTGCTAGGGATAGTTCGGCATTCTGAAAGTGAATCTAAGAGAAACAGGCTGCTCGGAGGAGATGCCAAACTAGCTCCTGCTTCCTTATCGTTGGACTACAGTACAGTGAGTACACAGTCCAAAAGTTTTCTTGTGAGGATAGTTCATGCAGGTGGACAAGAGGAGCTGTTTCCGGATGCAGTTCCTGCGTCTTTGTTGATGGACAAGTATCCTGGGAAATGTGTTGCCCGCCCAGATGTTTTCAAAAAGCCTCAGGAGTCCTTTTTAAGGCCAGAGGAGAGCCTATTGCCTGGACAGAAGTATTATATAATACCATGTACAACAGCGCAGAAACTTAAGCGTAAACAACAGAATAAGGTGAAGCCCAAAGAACCTGCTGAAGATGAAGAGATGATTCTGGGTGGAAAGATCACTGCGGATTCAGAAGGGGAAGAAACTGATGATTCTGTTTGCTCCGCTAGTGACTTTGTCTCCAAAGAAAAGAGGTCAAAATCTTCACGCAGGAGGGGAATTAGAGGAAAGAAGCCGTTTGTACCTCCCCTCCCAAAAGCCAGATCAGGTGGTAAAGGATTTGGATGGGAGCCTAGTCTCACGTCTAGCACCGGCAGCTCCACAGCGCCTAGAGCCTTTGCCAATGCCAAAGGCAAGCTTGATCCAAGGGGGAAAGCGTTGCAAATGAAATTGCAGTTACACCCACAAGCACATCAACTAGTGTTGCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

245

Amino Acids

26.72

Weight (kDa)

9.67

Isoelectric Point (pI)

58.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 44 - 203 5.6e-15 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017693)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g24840
malus_domestica MD13G1101500.v1.1
prunus_persica Prupe.1G244300_v2.0.a1
pyrus_communis pycom13g08860 pycom16g08660
rosa_laevigata RLG00000006797
rosa_rugosa Rorug04G0264400
rosa_samantha Rh4AG318300 Rh4BG326400 Rh4CG341700 Rh4DG321600
rosa_wichuraiana Rw4G027670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 152
Acc36I ACCTGC 2 cut(s) 152, 394
AccIII TCCGGA 1 cut(s) 184
AciI CCGC 3 cut(s) 241, 428, 468
AclWI GGATC 1 cut(s) 659
AcuI CTGAAG 1 cut(s) 411
AfaI GTAC 4 cut(s) 115, 124, 331, 546
AfiI CCNNNNNNNGG 1 cut(s) 279
AflII CTTAAG 1 cut(s) 347
AgsI TTSAA 1 cut(s) 256
AhlI ACTAGT 1 cut(s) 721
AjiI CACGTC 1 cut(s) 606
AjnI CCWGG 2 cut(s) 223, 301
AluBI AGCT 4 cut(s) 86, 178, 621, 661
AluI AGCT 4 cut(s) 86, 178, 621, 661
Alw26I GTCTC 2 cut(s) 487, 605
AlwI GGATC 1 cut(s) 659
AlwNI CAGNNNCTG 1 cut(s) 197
Aor13HI TCCGGA 1 cut(s) 184
AoxI GGCC 1 cut(s) 281
ApeKI GCWGC 2 cut(s) 61, 618
AspLEI GCGC 2 cut(s) 340, 631
AsuHPI GGTGA 1 cut(s) 382
AxyI CCTNAGG 1 cut(s) 264
BbvI GCAGC 2 cut(s) 48, 630
BccI CCATC 2 cut(s) 205, 582
BceAI ACGGC 2 cut(s) 523, 715
BciT130I CCWGG 2 cut(s) 225, 303
BciVI GTATCC 1 cut(s) 231
BcoDI GTCTC 2 cut(s) 487, 605
BcuI ACTAGT 1 cut(s) 721
BfaI CTAG 7 cut(s) 17, 83, 471, 597, 609, 633, 722
BfmI CTRYAG 1 cut(s) 109
BfoI RGCGCY 1 cut(s) 632
BfrI CTTAAG 1 cut(s) 347
BfuAI ACCTGC 2 cut(s) 152, 394
BfuI GTATCC 1 cut(s) 231
BisI GCNGC 2 cut(s) 62, 619
BlsI GCNGC 2 cut(s) 63, 620
Bme1390I CCNGG 2 cut(s) 225, 303
BmgBI CACGTC 1 cut(s) 606
BmiI GGNNCC 1 cut(s) 594
BmrFI CCNGG 2 cut(s) 225, 303
BmsI GCATC 2 cut(s) 64, 178
BsaJI CCNNGG 2 cut(s) 224, 668
BsaWI WCCGGW 1 cut(s) 184
BsaXI ACNNNNNCTCC 4 cut(s) 467, 497, 537, 567
Bsc4I CCNNNNNNNGG 1 cut(s) 279
Bse118I RCCGGY 1 cut(s) 614
Bse21I CCTNAGG 1 cut(s) 264
BseAI TCCGGA 1 cut(s) 184
BseBI CCWGG 2 cut(s) 225, 303
BseDI CCNNGG 2 cut(s) 224, 668
BseGI GGATG 2 cut(s) 193, 593
BseLI CCNNNNNNNGG 1 cut(s) 279
BseMII CTCAG 1 cut(s) 278
BseRI GAGGAG 3 cut(s) 84, 188, 302
BseXI GCAGC 2 cut(s) 48, 630
BshFI GGCC 1 cut(s) 283
BsiSI CCGG 2 cut(s) 185, 615
BslI CCNNNNNNNGG 1 cut(s) 279
BsmAI GTCTC 2 cut(s) 487, 605
BsmI GAATGC 1 cut(s) 31
BsnI GGCC 1 cut(s) 283
Bsp13I TCCGGA 1 cut(s) 184
Bsp1407I TGTACA 1 cut(s) 329
Bsp143I GATC 3 cut(s) 420, 566, 664
BspACI CCGC 3 cut(s) 241, 428, 468
BspANI GGCC 1 cut(s) 283
BspCNI CTCAG 1 cut(s) 277
BspEI TCCGGA 1 cut(s) 184
BspLI GGNNCC 1 cut(s) 594
BspMI ACCTGC 2 cut(s) 152, 394
BspPI GGATC 1 cut(s) 659
BspTI CTTAAG 1 cut(s) 347
BsrFI RCCGGY 1 cut(s) 614
BsrGI TGTACA 1 cut(s) 329
BssAI RCCGGY 1 cut(s) 614
BssECI CCNNGG 2 cut(s) 224, 668
BssMI GATC 3 cut(s) 420, 566, 664
BssT1I CCWWGG 1 cut(s) 668
Bst2UI CCWGG 2 cut(s) 225, 303
Bst4CI ACNGT 3 cut(s) 113, 118, 129
Bst6I CTCTTC 1 cut(s) 393
BstAFI CTTAAG 1 cut(s) 347
BstAPI GCANNNNNTGC 1 cut(s) 197
BstAUI TGTACA 1 cut(s) 329
BstC8I GCNNGC 3 cut(s) 8, 241, 659
BstDEI CTNAG 2 cut(s) 48, 264
BstENI CCTNNNNNAGG 1 cut(s) 277
BstF5I GGATG 2 cut(s) 193, 593
BstH2I RGCGCY 1 cut(s) 632
BstHHI GCGC 2 cut(s) 340, 631
BstKTI GATC 3 cut(s) 423, 569, 667
BstMAI GTCTC 2 cut(s) 487, 605
BstMBI GATC 3 cut(s) 420, 566, 664
BstMWI GCNNNNNNNGC 4 cut(s) 83, 197, 618, 635
BstNI CCWGG 2 cut(s) 225, 303
BstNSI RCATGY 1 cut(s) 10
BstSCI CCNGG 2 cut(s) 223, 301
BstSFI CTRYAG 1 cut(s) 109
BstV1I GCAGC 2 cut(s) 48, 630
Bsu36I CCTNAGG 1 cut(s) 264
BsuI GTATCC 1 cut(s) 231
BsuRI GGCC 1 cut(s) 283
BtrI CACGTC 1 cut(s) 606
BtsCI GGATG 2 cut(s) 193, 593
BtsI GCAGTG 1 cut(s) 423
BtsIMutI CAGTG 2 cut(s) 123, 423
BveI ACCTGC 2 cut(s) 152, 394
Cac8I GCNNGC 3 cut(s) 8, 241, 659
CaiI CAGNNNCTG 1 cut(s) 197
CfoI GCGC 2 cut(s) 340, 631
Cfr10I RCCGGY 1 cut(s) 614
CseI GACGC 1 cut(s) 189
Csp6I GTAC 4 cut(s) 114, 123, 330, 545
CviAII CATG 3 cut(s) 7, 158, 327
CviQI GTAC 4 cut(s) 114, 123, 330, 545
DdeI CTNAG 2 cut(s) 48, 264
DpnI GATC 3 cut(s) 422, 568, 666
DpnII GATC 3 cut(s) 420, 566, 664
Eam1104I CTCTTC 1 cut(s) 393
EarI CTCTTC 1 cut(s) 393
Eco130I CCWWGG 1 cut(s) 668
Eco57I CTGAAG 1 cut(s) 411
Eco81I CCTNAGG 1 cut(s) 264
EcoNI CCTNNNNNAGG 1 cut(s) 277
EcoRII CCWGG 2 cut(s) 223, 301
EcoT14I CCWWGG 1 cut(s) 668
ErhI CCWWGG 1 cut(s) 668
FaeI CATG 3 cut(s) 10, 161, 330
FaiI YATR 5 cut(s) 8, 159, 318, 320, 328
FalI AAGNNNNNCTT 2 cut(s) 645, 677
FatI CATG 3 cut(s) 6, 157, 326
FauI CCCGC 1 cut(s) 248
Fnu4HI GCNGC 2 cut(s) 62, 619
FokI GGATG 2 cut(s) 200, 600
Fsp4HI GCNGC 2 cut(s) 62, 619
FspBI CTAG 7 cut(s) 17, 83, 471, 597, 609, 633, 722
GlaI GCGC 2 cut(s) 339, 630
GluI GCNGC 2 cut(s) 62, 619
HaeII RGCGCY 1 cut(s) 632
HaeIII GGCC 1 cut(s) 283
HapII CCGG 2 cut(s) 185, 615
HgaI GACGC 1 cut(s) 189
HhaI GCGC 2 cut(s) 340, 631
Hin1II CATG 3 cut(s) 10, 161, 330
Hin6I GCGC 2 cut(s) 338, 629
HinP1I GCGC 2 cut(s) 338, 629
HindIII AAGCTT 1 cut(s) 659
HinfI GANTC 5 cut(s) 44, 269, 406, 431, 455
HpaII CCGG 2 cut(s) 185, 615
HphI GGTGA 1 cut(s) 382
Hpy166II GTNNAC 3 cut(s) 125, 167, 356
Hpy188I TCNGA 3 cut(s) 37, 68, 436
Hpy188III TCNNGA 2 cut(s) 185, 266
Hpy8I GTNNAC 3 cut(s) 125, 167, 356
HpyAV CCTTC 1 cut(s) 431
HpyCH4III ACNGT 3 cut(s) 113, 118, 129
HpyCH4IV ACGT 1 cut(s) 605
HpyCH4V TGCA 6 cut(s) 6, 10, 161, 191, 685, 697
HpyF10VI GCNNNNNNNGC 4 cut(s) 83, 197, 618, 635
HpyF3I CTNAG 2 cut(s) 48, 264
HpySE526I ACGT 1 cut(s) 605
Hsp92II CATG 3 cut(s) 10, 161, 330
HspAI GCGC 2 cut(s) 338, 629
Kpn2I TCCGGA 1 cut(s) 184
Kzo9I GATC 3 cut(s) 420, 566, 664
LmnI GCTCC 5 cut(s) 91, 175, 470, 592, 626
Lsp1109I GCAGC 2 cut(s) 48, 630
LweI GCATC 2 cut(s) 64, 178
MaeI CTAG 7 cut(s) 17, 83, 471, 597, 609, 633, 722
MaeII ACGT 1 cut(s) 605
MaeIII GTNAC 2 cut(s) 473, 699
MalI GATC 3 cut(s) 422, 568, 666
MboI GATC 3 cut(s) 420, 566, 664
MboII GAAGA 4 cut(s) 404, 410, 455, 498
MluCI AATT 2 cut(s) 522, 692
MlyI GAGTC 1 cut(s) 278
MmeI TCCRAC 1 cut(s) 84
MroI TCCGGA 1 cut(s) 184
MseI TTAA 2 cut(s) 278, 348
MslI CAYNNNNRTG 1 cut(s) 162
MspCI CTTAAG 1 cut(s) 347
MspI CCGG 2 cut(s) 185, 615
MspR9I CCNGG 2 cut(s) 225, 303
Mva1269I GAATGC 1 cut(s) 31
MvaI CCWGG 2 cut(s) 225, 303
MwoI GCNNNNNNNGC 4 cut(s) 83, 197, 618, 635
NdeII GATC 3 cut(s) 420, 566, 664
NlaIII CATG 3 cut(s) 10, 161, 330
NlaIV GGNNCC 1 cut(s) 594
NmuCI GTSAC 1 cut(s) 473
NspI RCATGY 1 cut(s) 10
PaeI GCATGC 1 cut(s) 10
PaqCI CACCTGC 1 cut(s) 152
PctI GAATGC 1 cut(s) 31
PfeI GAWTC 4 cut(s) 44, 406, 431, 455
PflFI GACNNNGTC 1 cut(s) 479
PkrI GCNGC 2 cut(s) 63, 620
PleI GAGTC 1 cut(s) 277
PpsI GAGTC 1 cut(s) 277
Psp6I CCWGG 2 cut(s) 223, 301
PspGI CCWGG 2 cut(s) 223, 301
PspN4I GGNNCC 1 cut(s) 594
PstNI CAGNNNCTG 1 cut(s) 197
PsyI GACNNNGTC 1 cut(s) 479
RsaI GTAC 4 cut(s) 115, 124, 331, 546
RsaNI GTAC 4 cut(s) 114, 123, 330, 545
RseI CAYNNNNRTG 1 cut(s) 162
SaqAI TTAA 2 cut(s) 278, 348
SatI GCNGC 2 cut(s) 62, 619
Sau3AI GATC 3 cut(s) 420, 566, 664
SchI GAGTC 1 cut(s) 278
ScrFI CCNGG 2 cut(s) 225, 303
SfaNI GCATC 2 cut(s) 64, 178
SfcI CTRYAG 1 cut(s) 109
SmiMI CAYNNNNRTG 1 cut(s) 162
SmlI CTYRAG 1 cut(s) 347
SmoI CTYRAG 1 cut(s) 347
SpeI ACTAGT 1 cut(s) 721
SphI GCATGC 1 cut(s) 10
Sse9I AATT 2 cut(s) 522, 692
SsiI CCGC 3 cut(s) 241, 428, 468
SspMI CTAG 7 cut(s) 17, 83, 471, 597, 609, 633, 722
StyD4I CCNGG 2 cut(s) 223, 301
StyI CCWWGG 1 cut(s) 668
TaaI ACNGT 3 cut(s) 113, 118, 129
TaiI ACGT 1 cut(s) 608
TasI AATT 2 cut(s) 522, 692
TatI WGTACW 3 cut(s) 113, 122, 329
TfiI GAWTC 4 cut(s) 44, 406, 431, 455
Tru1I TTAA 2 cut(s) 278, 348
Tru9I TTAA 2 cut(s) 278, 348
TscAI CASTG 2 cut(s) 123, 430
TseFI GTSAC 1 cut(s) 473
TseI GCWGC 2 cut(s) 61, 618
Tsp45I GTSAC 1 cut(s) 473
TspDTI ATGAA 3 cut(s) 146, 411, 704
TspRI CASTG 2 cut(s) 123, 430
Tth111I GACNNNGTC 1 cut(s) 479
Vha464I CTTAAG 1 cut(s) 347
XagI CCTNNNNNAGG 1 cut(s) 277
XceI RCATGY 1 cut(s) 10
XspI CTAG 7 cut(s) 17, 83, 471, 597, 609, 633, 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.