MD13G1101500.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0017693
Physical Location & Seq
Reverse (-)
7223741 .. 7224638
898 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1101500.v1.1.491

Sequence Viewer

Length: 639 bp
ATGCTGAAGTGGTGCATCCAGTTGTTAGGCCTAGCCCGGAATCCTGGCAGAAATCAGTTGACAGGACCTTCCATGGATGACAAATCCAAAAGAAAGGAAGTAGATGCTGCTTCCATATCATTAGACTTGAGCATGCAGTCGAAGGATATTTTCGTGAGGATAGTTCATGCAGGCGGTCGTGAGGAGCTGTACCAGAATGTAGTTCCTGCATCTCAGTTGATGGAAAAGTATCCTGGTTTGTGCGTCGCCCGCCCAGAAGTGTTCAAAAATCCGCAGGAGGCCCTTTTACGGCCAGATGAGATTCTGTTGATTGGTAAGAAGTATTACATGATACCATGTACTACAGCGCAGAAAATTAAGAAAAAACACCAAAAGAAATTGAAGGCCAAAGAAGCTGCTGAAGGCAAAGATGAGGCTCCGGATGGGAAGAACACCGTGGATTCAGGAGGTTCCGACACAGATGATTCTGTTTGCTCCGATAAAGAATATTATGTCTCCAAGGAAAGATGGTCAAAATGTTTACGGAGAAAAGGTTTCAGAGGAAAGAAGCCTTTTGTTCCCCCACTTCCGAGGACAAGATCATGTCGAGGAATAAATTGGGAGCCAAGTCTCACTTCTGTGCAAGAGCTTTCTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

213

Amino Acids

23.88

Weight (kDa)

9.43

Isoelectric Point (pI)

41.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 49 - 209 8.6e-18 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017693)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g24840
malus_domestica MD13G1101500.v1.1
prunus_persica Prupe.1G244300_v2.0.a1
pyrus_communis pycom13g08860 pycom16g08660
rosa_laevigata RLG00000006797
rosa_rugosa Rorug04G0264400
rosa_samantha Rh4AG318300 Rh4BG326400 Rh4CG341700 Rh4DG321600
rosa_wichuraiana Rw4G027670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 418
AciI CCGC 3 cut(s) 174, 250, 272
AcoI YGGCCR 1 cut(s) 290
AcuI CTGAAG 2 cut(s) 26, 420
AfaI GTAC 2 cut(s) 191, 340
AfiI CCNNNNNNNGG 1 cut(s) 288
AgsI TTSAA 2 cut(s) 265, 382
AjnI CCWGG 2 cut(s) 43, 232
AjuI GAANNNNNNNTTGG 2 cut(s) 598, 630
AleI CACNNNNGTG 1 cut(s) 617
AluBI AGCT 3 cut(s) 187, 395, 628
AluI AGCT 3 cut(s) 187, 395, 628
Alw26I GTCTC 2 cut(s) 499, 614
Aor13HI TCCGGA 1 cut(s) 418
AoxI GGCC 4 cut(s) 28, 279, 290, 384
ApeKI GCWGC 2 cut(s) 107, 395
AspLEI GCGC 1 cut(s) 349
AspS9I GGNCC 2 cut(s) 65, 280
AsuC2I CCSGG 1 cut(s) 37
AvaII GGWCC 1 cut(s) 65
BbvI GCAGC 2 cut(s) 94, 382
BccI CCATC 3 cut(s) 214, 416, 501
BceAI ACGGC 1 cut(s) 305
BciT130I CCWGG 2 cut(s) 45, 234
BciVI GTATCC 1 cut(s) 240
BcnI CCSGG 1 cut(s) 37
BcoDI GTCTC 2 cut(s) 499, 614
BfaI CTAG 1 cut(s) 32
BfmI CTRYAG 1 cut(s) 342
BfuI GTATCC 1 cut(s) 240
BisI GCNGC 2 cut(s) 108, 396
BlsI GCNGC 2 cut(s) 109, 397
Bme1390I CCNGG 3 cut(s) 37, 45, 234
Bme18I GGWCC 1 cut(s) 65
BmgT120I GGNCC 2 cut(s) 65, 280
BmiI GGNNCC 3 cut(s) 417, 451, 603
BmrFI CCNGG 3 cut(s) 37, 45, 234
BmsI GCATC 3 cut(s) 24, 94, 218
BpuEI CTTGAG 1 cut(s) 148
BpuMI CCSGG 1 cut(s) 37
BsaJI CCNNGG 4 cut(s) 72, 435, 498, 569
BsaWI WCCGGW 1 cut(s) 418
BsaXI ACNNNNNCTCC 2 cut(s) 517, 547
Bsc4I CCNNNNNNNGG 1 cut(s) 288
Bse1I ACTGG 1 cut(s) 19
BseAI TCCGGA 1 cut(s) 418
BseBI CCWGG 2 cut(s) 45, 234
BseDI CCNNGG 4 cut(s) 72, 435, 498, 569
BseGI GGATG 3 cut(s) 15, 82, 427
BseLI CCNNNNNNNGG 1 cut(s) 288
BseMII CTCAG 1 cut(s) 227
BseNI ACTGG 1 cut(s) 19
BseRI GAGGAG 1 cut(s) 197
BseXI GCAGC 2 cut(s) 94, 382
Bsh1285I CGRYCG 1 cut(s) 178
BshFI GGCC 4 cut(s) 30, 281, 292, 386
BsiEI CGRYCG 1 cut(s) 178
BsiSI CCGG 2 cut(s) 37, 419
BslI CCNNNNNNNGG 1 cut(s) 288
BsmAI GTCTC 2 cut(s) 499, 614
BsnI GGCC 4 cut(s) 30, 281, 292, 386
Bsp13I TCCGGA 1 cut(s) 418
Bsp143I GATC 1 cut(s) 578
Bsp19I CCATGG 1 cut(s) 72
BspACI CCGC 3 cut(s) 174, 250, 272
BspANI GGCC 4 cut(s) 30, 281, 292, 386
BspCNI CTCAG 1 cut(s) 226
BspEI TCCGGA 1 cut(s) 418
BspLI GGNNCC 3 cut(s) 417, 451, 603
BsrI ACTGG 1 cut(s) 19
BssECI CCNNGG 4 cut(s) 72, 435, 498, 569
BssMI GATC 1 cut(s) 578
BssT1I CCWWGG 2 cut(s) 72, 498
Bst2UI CCWGG 2 cut(s) 45, 234
Bst4CI ACNGT 1 cut(s) 436
BstC8I GCNNGC 3 cut(s) 134, 172, 250
BstDEI CTNAG 1 cut(s) 213
BstDSI CCRYGG 2 cut(s) 72, 435
BstF5I GGATG 3 cut(s) 15, 82, 427
BstHHI GCGC 1 cut(s) 349
BstKTI GATC 1 cut(s) 581
BstMAI GTCTC 2 cut(s) 499, 614
BstMBI GATC 1 cut(s) 578
BstMCI CGRYCG 1 cut(s) 178
BstMWI GCNNNNNNNGC 2 cut(s) 249, 392
BstNI CCWGG 2 cut(s) 45, 234
BstNSI RCATGY 1 cut(s) 136
BstSCI CCNGG 3 cut(s) 35, 43, 232
BstSFI CTRYAG 1 cut(s) 342
BstV1I GCAGC 2 cut(s) 94, 382
BsuI GTATCC 1 cut(s) 240
BsuRI GGCC 4 cut(s) 30, 281, 292, 386
BtgI CCRYGG 2 cut(s) 72, 435
BtsCI GGATG 3 cut(s) 15, 82, 427
Cac8I GCNNGC 3 cut(s) 134, 172, 250
CfoI GCGC 1 cut(s) 349
Cfr13I GGNCC 2 cut(s) 65, 280
CseI GACGC 1 cut(s) 232
Csp6I GTAC 2 cut(s) 190, 339
CviAII CATG 7 cut(s) 73, 133, 167, 328, 336, 582, 636
CviQI GTAC 2 cut(s) 190, 339
DdeI CTNAG 1 cut(s) 213
DpnI GATC 1 cut(s) 580
DpnII GATC 1 cut(s) 578
EaeI YGGCCR 1 cut(s) 290
Eco130I CCWWGG 2 cut(s) 72, 498
Eco147I AGGCCT 1 cut(s) 30
Eco47I GGWCC 1 cut(s) 65
Eco57I CTGAAG 2 cut(s) 26, 420
EcoO109I RGGNCCY 2 cut(s) 65, 280
EcoRII CCWGG 2 cut(s) 43, 232
EcoT14I CCWWGG 2 cut(s) 72, 498
ErhI CCWWGG 2 cut(s) 72, 498
FaeI CATG 7 cut(s) 76, 136, 170, 331, 339, 585, 639
FaiI YATR 9 cut(s) 74, 116, 134, 168, 329, 337, 492, 583, 637
FalI AAGNNNNNCTT 2 cut(s) 598, 630
FatI CATG 7 cut(s) 72, 132, 166, 327, 335, 581, 635
FauI CCCGC 1 cut(s) 257
Fnu4HI GCNGC 2 cut(s) 108, 396
FokI GGATG 3 cut(s) 2, 89, 434
Fsp4HI GCNGC 2 cut(s) 108, 396
FspBI CTAG 1 cut(s) 32
GlaI GCGC 1 cut(s) 348
GluI GCNGC 2 cut(s) 108, 396
HaeIII GGCC 4 cut(s) 30, 281, 292, 386
HapII CCGG 2 cut(s) 37, 419
HgaI GACGC 1 cut(s) 232
HhaI GCGC 1 cut(s) 349
Hin1II CATG 7 cut(s) 76, 136, 170, 331, 339, 585, 639
Hin6I GCGC 1 cut(s) 347
HinP1I GCGC 1 cut(s) 347
HincII GTYRAC 1 cut(s) 60
HindII GTYRAC 1 cut(s) 60
HinfI GANTC 4 cut(s) 40, 301, 440, 464
HpaII CCGG 2 cut(s) 37, 419
Hpy166II GTNNAC 2 cut(s) 60, 521
Hpy188I TCNGA 4 cut(s) 454, 478, 539, 570
Hpy188III TCNNGA 4 cut(s) 154, 179, 419, 444
Hpy8I GTNNAC 2 cut(s) 60, 521
Hpy99I CGWCG 1 cut(s) 248
HpyAV CCTTC 4 cut(s) 78, 136, 376, 395
HpyCH4III ACNGT 1 cut(s) 436
HpyCH4V TGCA 5 cut(s) 15, 136, 170, 209, 622
HpyF10VI GCNNNNNNNGC 2 cut(s) 249, 392
HpyF3I CTNAG 1 cut(s) 213
Hsp92II CATG 7 cut(s) 76, 136, 170, 331, 339, 585, 639
HspAI GCGC 1 cut(s) 347
Kpn2I TCCGGA 1 cut(s) 418
Kzo9I GATC 1 cut(s) 578
LmnI GCTCC 4 cut(s) 184, 421, 479, 601
Lsp1109I GCAGC 2 cut(s) 94, 382
LweI GCATC 3 cut(s) 24, 94, 218
MaeI CTAG 1 cut(s) 32
MalI GATC 1 cut(s) 580
MboI GATC 1 cut(s) 578
MboII GAAGA 1 cut(s) 439
MluCI AATT 3 cut(s) 354, 377, 595
MmeI TCCRAC 1 cut(s) 477
MnlI CCTC 8 cut(s) 150, 175, 271, 406, 440, 533, 564, 581
MroI TCCGGA 1 cut(s) 418
MseI TTAA 1 cut(s) 357
MslI CAYNNNNRTG 1 cut(s) 617
MspI CCGG 2 cut(s) 37, 419
MspR9I CCNGG 3 cut(s) 37, 45, 234
MvaI CCWGG 2 cut(s) 45, 234
MwoI GCNNNNNNNGC 2 cut(s) 249, 392
NciI CCSGG 1 cut(s) 37
NcoI CCATGG 1 cut(s) 72
NdeII GATC 1 cut(s) 578
NlaIII CATG 7 cut(s) 76, 136, 170, 331, 339, 585, 639
NlaIV GGNNCC 3 cut(s) 417, 451, 603
NspI RCATGY 1 cut(s) 136
OliI CACNNNNGTG 1 cut(s) 617
PaeI GCATGC 1 cut(s) 136
PceI AGGCCT 1 cut(s) 30
PfeI GAWTC 4 cut(s) 40, 301, 440, 464
PkrI GCNGC 2 cut(s) 109, 397
PpuMI RGGWCCY 1 cut(s) 65
Psp5II RGGWCCY 1 cut(s) 65
Psp6I CCWGG 2 cut(s) 43, 232
PspGI CCWGG 2 cut(s) 43, 232
PspN4I GGNNCC 3 cut(s) 417, 451, 603
PspPI GGNCC 2 cut(s) 65, 280
PspPPI RGGWCCY 1 cut(s) 65
RsaI GTAC 2 cut(s) 191, 340
RsaNI GTAC 2 cut(s) 190, 339
RseI CAYNNNNRTG 1 cut(s) 617
SaqAI TTAA 1 cut(s) 357
SatI GCNGC 2 cut(s) 108, 396
Sau3AI GATC 1 cut(s) 578
Sau96I GGNCC 2 cut(s) 65, 280
ScrFI CCNGG 3 cut(s) 37, 45, 234
SetI ASST 6 cut(s) 70, 189, 397, 451, 535, 630
SfaNI GCATC 3 cut(s) 24, 94, 218
SfcI CTRYAG 1 cut(s) 342
SinI GGWCC 1 cut(s) 65
SmiMI CAYNNNNRTG 1 cut(s) 617
SmlI CTYRAG 1 cut(s) 127
SmoI CTYRAG 1 cut(s) 127
SphI GCATGC 1 cut(s) 136
Sse9I AATT 3 cut(s) 354, 377, 595
SseBI AGGCCT 1 cut(s) 30
SsiI CCGC 3 cut(s) 174, 250, 272
SspI AATATT 1 cut(s) 488
SspMI CTAG 1 cut(s) 32
StuI AGGCCT 1 cut(s) 30
StyD4I CCNGG 3 cut(s) 35, 43, 232
StyI CCWWGG 2 cut(s) 72, 498
TaaI ACNGT 1 cut(s) 436
TaqI TCGA 2 cut(s) 140, 586
TasI AATT 3 cut(s) 354, 377, 595
TatI WGTACW 1 cut(s) 338
TfiI GAWTC 4 cut(s) 40, 301, 440, 464
Tru1I TTAA 1 cut(s) 357
Tru9I TTAA 1 cut(s) 357
TseI GCWGC 2 cut(s) 107, 395
TspDTI ATGAA 1 cut(s) 155
TspGWI ACGGA 1 cut(s) 538
VpaK11BI GGWCC 1 cut(s) 65
XceI RCATGY 1 cut(s) 136
XspI CTAG 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.