Rw4G027670

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0017693
Physical Location & Seq
Forward (+)
54246333 .. 54246962
630 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G027670.1

Sequence Viewer

Length: 630 bp
ATGCTAAAGATGTGCATGCAATTATTAGGGCTAGTTCGGCATACTGAAAGTGAATCTAAGAGAAACAAGCTGCTCGGACGAGATGCCAATCAAGCTCCTGCTTCCTTATCATTGGACTACAGTACACAGTCCAAAAGTATTCTTGTGAGGATAGTTCATGCAGGTGGGCAAGAGGAGCTGTTTCCGAATGCAGTTCCTGCATCTCTGTTGATGGACAAATATCCTGGGAAATGTGTTGCCCGCCCAGAAGTTTTCAAAAAGCCTCAGGAGTCCCTCTTAAGGCCAGACGAGAGCCTATTGCCTGGTCAGAAGTATTATATAATACCATGTACAACAGCACAGAAGCTTAAGCGTCAACAGCAGAAGCAGGTGAAGGCCAAAGAACGTGCTGAAGATGAAGAGACTGTGGATTCGGAAGGAAAGATCACTGTGGATTCGGAAGGGGAAGACACTGATGATTCTATTTGCTCTGCCAGTGACTTCGTCTCCAAGGAAAAGAGACCAAAATCTTCAAGGAGGAGGGGAATTAGAGGAAGAAAGCCGTTTGTACCTCCCCTCCCAAAGGTCAGATCTGGTAGAGGATTTGGATGGGAGCCTAGTCTCACTTCTGTACAAGAAGTGTCTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

209

Amino Acids

23.34

Weight (kDa)

9.33

Isoelectric Point (pI)

63.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 43 - 206 1.8e-17 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017693)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g24840
malus_domestica MD13G1101500.v1.1
prunus_persica Prupe.1G244300_v2.0.a1
pyrus_communis pycom13g08860 pycom16g08660
rosa_laevigata RLG00000006797
rosa_rugosa Rorug04G0264400
rosa_samantha Rh4AG318300 Rh4BG326400 Rh4CG341700 Rh4DG321600
rosa_wichuraiana Rw4G027670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 152, 358
Acc36I ACCTGC 2 cut(s) 152, 358
AciI CCGC 1 cut(s) 241
AcuI CTGAAG 1 cut(s) 411
AfaI GTAC 4 cut(s) 124, 331, 549, 612
AfiI CCNNNNNNNGG 2 cut(s) 279, 562
AflII CTTAAG 2 cut(s) 277, 347
AgsI TTSAA 2 cut(s) 256, 513
AjnI CCWGG 2 cut(s) 223, 301
AluBI AGCT 4 cut(s) 70, 95, 178, 346
AluI AGCT 4 cut(s) 70, 95, 178, 346
Alw26I GTCTC 4 cut(s) 395, 490, 493, 605
AlwNI CAGNNNCTG 1 cut(s) 197
AoxI GGCC 2 cut(s) 281, 375
ApeKI GCWGC 1 cut(s) 70
ArsI GACNNNNNNTTYG 2 cut(s) 394, 426
AsuHPI GGTGA 1 cut(s) 382
AxyI CCTNAGG 1 cut(s) 264
BbsI GAAGAC 1 cut(s) 453
BbvI GCAGC 1 cut(s) 57
BccI CCATC 2 cut(s) 205, 582
BceAI ACGGC 1 cut(s) 526
BciT130I CCWGG 2 cut(s) 225, 303
BcoDI GTCTC 4 cut(s) 395, 490, 493, 605
BfaI CTAG 2 cut(s) 32, 597
BfmI CTRYAG 1 cut(s) 118
BfrI CTTAAG 2 cut(s) 277, 347
BfuAI ACCTGC 2 cut(s) 152, 358
BglII AGATCT 1 cut(s) 569
BisI GCNGC 1 cut(s) 71
BlsI GCNGC 1 cut(s) 72
Bme1390I CCNGG 2 cut(s) 225, 303
BmiI GGNNCC 1 cut(s) 594
BmrFI CCNGG 2 cut(s) 225, 303
BmsI GCATC 2 cut(s) 73, 209
BpiI GAAGAC 1 cut(s) 453
BsaBI GATNNNNATC 1 cut(s) 87
BsaI GGTCTC 1 cut(s) 493
BsaJI CCNNGG 2 cut(s) 224, 489
BsaXI ACNNNNNCTCC 4 cut(s) 470, 500, 540, 570
Bsc4I CCNNNNNNNGG 2 cut(s) 279, 562
Bse1I ACTGG 1 cut(s) 474
Bse21I CCTNAGG 1 cut(s) 264
Bse8I GATNNNNATC 1 cut(s) 87
BseBI CCWGG 2 cut(s) 225, 303
BseDI CCNNGG 2 cut(s) 224, 489
BseGI GGATG 1 cut(s) 593
BseJI GATNNNNATC 1 cut(s) 87
BseLI CCNNNNNNNGG 2 cut(s) 279, 562
BseMII CTCAG 1 cut(s) 278
BseNI ACTGG 1 cut(s) 474
BseRI GAGGAG 2 cut(s) 188, 532
BseXI GCAGC 1 cut(s) 57
BshFI GGCC 2 cut(s) 283, 377
BslFI GGGAC 1 cut(s) 256
BslI CCNNNNNNNGG 2 cut(s) 279, 562
BsmAI GTCTC 4 cut(s) 395, 490, 493, 605
BsmBI CGTCTC 1 cut(s) 490
BsmFI GGGAC 1 cut(s) 256
BsmI GAATGC 1 cut(s) 193
BsnI GGCC 2 cut(s) 283, 377
Bso31I GGTCTC 1 cut(s) 493
Bsp1407I TGTACA 2 cut(s) 329, 610
Bsp143I GATC 2 cut(s) 423, 569
BspACI CCGC 1 cut(s) 241
BspANI GGCC 2 cut(s) 283, 377
BspCNI CTCAG 1 cut(s) 277
BspLI GGNNCC 1 cut(s) 594
BspMI ACCTGC 2 cut(s) 152, 358
BspTI CTTAAG 2 cut(s) 277, 347
BspTNI GGTCTC 1 cut(s) 493
BsrGI TGTACA 2 cut(s) 329, 610
BsrI ACTGG 1 cut(s) 474
BssECI CCNNGG 2 cut(s) 224, 489
BssMI GATC 2 cut(s) 423, 569
BssT1I CCWWGG 1 cut(s) 489
Bst2UI CCWGG 2 cut(s) 225, 303
Bst4CI ACNGT 4 cut(s) 122, 129, 406, 430
Bst6I CTCTTC 1 cut(s) 393
BstAFI CTTAAG 2 cut(s) 277, 347
BstAPI GCANNNNNTGC 1 cut(s) 197
BstAUI TGTACA 2 cut(s) 329, 610
BstC8I GCNNGC 2 cut(s) 17, 241
BstDEI CTNAG 2 cut(s) 57, 264
BstENI CCTNNNNNAGG 1 cut(s) 560
BstF5I GGATG 1 cut(s) 593
BstKTI GATC 2 cut(s) 426, 572
BstMAI GTCTC 4 cut(s) 395, 490, 493, 605
BstMBI GATC 2 cut(s) 423, 569
BstMWI GCNNNNNNNGC 5 cut(s) 37, 92, 175, 197, 358
BstNI CCWGG 2 cut(s) 225, 303
BstNSI RCATGY 1 cut(s) 19
BstSCI CCNGG 2 cut(s) 223, 301
BstSFI CTRYAG 1 cut(s) 118
BstV1I GCAGC 1 cut(s) 57
BstV2I GAAGAC 1 cut(s) 453
BstX2I RGATCY 1 cut(s) 569
BstYI RGATCY 1 cut(s) 569
Bsu36I CCTNAGG 1 cut(s) 264
BsuRI GGCC 2 cut(s) 283, 377
BtsCI GGATG 1 cut(s) 593
BtsIMutI CAGTG 3 cut(s) 426, 450, 481
BveI ACCTGC 2 cut(s) 152, 358
Cac8I GCNNGC 2 cut(s) 17, 241
CaiI CAGNNNCTG 1 cut(s) 197
CseI GACGC 1 cut(s) 341
Csp6I GTAC 4 cut(s) 123, 330, 548, 611
CviAII CATG 4 cut(s) 16, 158, 327, 627
CviQI GTAC 4 cut(s) 123, 330, 548, 611
DdeI CTNAG 2 cut(s) 57, 264
DpnI GATC 2 cut(s) 425, 571
DpnII GATC 2 cut(s) 423, 569
Eam1104I CTCTTC 1 cut(s) 393
EarI CTCTTC 1 cut(s) 393
Eco130I CCWWGG 1 cut(s) 489
Eco31I GGTCTC 1 cut(s) 493
Eco57I CTGAAG 1 cut(s) 411
Eco81I CCTNAGG 1 cut(s) 264
EcoNI CCTNNNNNAGG 1 cut(s) 560
EcoRII CCWGG 2 cut(s) 223, 301
EcoT14I CCWWGG 1 cut(s) 489
ErhI CCWWGG 1 cut(s) 489
Esp3I CGTCTC 1 cut(s) 490
FaeI CATG 4 cut(s) 19, 161, 330, 630
FaiI YATR 7 cut(s) 17, 42, 159, 318, 320, 328, 628
FaqI GGGAC 1 cut(s) 256
FatI CATG 4 cut(s) 15, 157, 326, 626
FauI CCCGC 1 cut(s) 248
Fnu4HI GCNGC 1 cut(s) 71
FokI GGATG 1 cut(s) 600
Fsp4HI GCNGC 1 cut(s) 71
FspBI CTAG 2 cut(s) 32, 597
GluI GCNGC 1 cut(s) 71
HaeIII GGCC 2 cut(s) 283, 377
HgaI GACGC 1 cut(s) 341
Hin1II CATG 4 cut(s) 19, 161, 330, 630
HincII GTYRAC 1 cut(s) 356
HindII GTYRAC 1 cut(s) 356
HindIII AAGCTT 1 cut(s) 344
HinfI GANTC 5 cut(s) 53, 269, 410, 434, 458
HphI GGTGA 1 cut(s) 382
Hpy166II GTNNAC 2 cut(s) 125, 356
Hpy188I TCNGA 6 cut(s) 77, 186, 309, 415, 439, 569
Hpy188III TCNNGA 1 cut(s) 266
Hpy8I GTNNAC 2 cut(s) 125, 356
HpyAV CCTTC 3 cut(s) 367, 410, 434
HpyCH4III ACNGT 4 cut(s) 122, 129, 406, 430
HpyCH4IV ACGT 1 cut(s) 385
HpyCH4V TGCA 5 cut(s) 15, 19, 161, 191, 200
HpyF10VI GCNNNNNNNGC 5 cut(s) 37, 92, 175, 197, 358
HpyF3I CTNAG 2 cut(s) 57, 264
HpySE526I ACGT 1 cut(s) 385
Hsp92II CATG 4 cut(s) 19, 161, 330, 630
Kzo9I GATC 2 cut(s) 423, 569
LmnI GCTCC 3 cut(s) 100, 175, 592
Lsp1109I GCAGC 1 cut(s) 57
LweI GCATC 2 cut(s) 73, 209
MaeI CTAG 2 cut(s) 32, 597
MaeII ACGT 1 cut(s) 385
MaeIII GTNAC 1 cut(s) 476
MalI GATC 2 cut(s) 425, 571
MboI GATC 2 cut(s) 423, 569
MboII GAAGA 5 cut(s) 404, 410, 458, 501, 546
MflI RGATCY 1 cut(s) 569
MluCI AATT 2 cut(s) 20, 525
MlyI GAGTC 1 cut(s) 278
MseI TTAA 2 cut(s) 278, 348
MslI CAYNNNNRTG 1 cut(s) 162
MspCI CTTAAG 2 cut(s) 277, 347
MspR9I CCNGG 2 cut(s) 225, 303
Mva1269I GAATGC 1 cut(s) 193
MvaI CCWGG 2 cut(s) 225, 303
MwoI GCNNNNNNNGC 5 cut(s) 37, 92, 175, 197, 358
NdeII GATC 2 cut(s) 423, 569
NlaIII CATG 4 cut(s) 19, 161, 330, 630
NlaIV GGNNCC 1 cut(s) 594
NmuCI GTSAC 1 cut(s) 476
NspI RCATGY 1 cut(s) 19
PaeI GCATGC 1 cut(s) 19
PaqCI CACCTGC 2 cut(s) 152, 358
PctI GAATGC 1 cut(s) 193
PfeI GAWTC 4 cut(s) 53, 410, 434, 458
PflFI GACNNNGTC 1 cut(s) 482
PkrI GCNGC 1 cut(s) 72
PleI GAGTC 1 cut(s) 277
PpsI GAGTC 1 cut(s) 277
Psp6I CCWGG 2 cut(s) 223, 301
PspGI CCWGG 2 cut(s) 223, 301
PspN4I GGNNCC 1 cut(s) 594
PstNI CAGNNNCTG 1 cut(s) 197
PsuI RGATCY 1 cut(s) 569
PsyI GACNNNGTC 1 cut(s) 482
RsaI GTAC 4 cut(s) 124, 331, 549, 612
RsaNI GTAC 4 cut(s) 123, 330, 548, 611
RseI CAYNNNNRTG 1 cut(s) 162
SaqAI TTAA 2 cut(s) 278, 348
SatI GCNGC 1 cut(s) 71
Sau3AI GATC 2 cut(s) 423, 569
SchI GAGTC 1 cut(s) 278
ScrFI CCNGG 2 cut(s) 225, 303
SetI ASST 9 cut(s) 72, 97, 166, 180, 348, 372, 388, 553, 567
SfaNI GCATC 2 cut(s) 73, 209
SfcI CTRYAG 1 cut(s) 118
SmiMI CAYNNNNRTG 1 cut(s) 162
SmlI CTYRAG 2 cut(s) 277, 347
SmoI CTYRAG 2 cut(s) 277, 347
SphI GCATGC 1 cut(s) 19
Sse9I AATT 2 cut(s) 20, 525
SsiI CCGC 1 cut(s) 241
SspMI CTAG 2 cut(s) 32, 597
StyD4I CCNGG 2 cut(s) 223, 301
StyI CCWWGG 1 cut(s) 489
TaaI ACNGT 4 cut(s) 122, 129, 406, 430
TaiI ACGT 1 cut(s) 388
TasI AATT 2 cut(s) 20, 525
TatI WGTACW 3 cut(s) 122, 329, 610
TfiI GAWTC 4 cut(s) 53, 410, 434, 458
Tru1I TTAA 2 cut(s) 278, 348
Tru9I TTAA 2 cut(s) 278, 348
TscAI CASTG 3 cut(s) 433, 457, 481
TseFI GTSAC 1 cut(s) 476
TseI GCWGC 1 cut(s) 70
Tsp45I GTSAC 1 cut(s) 476
TspDTI ATGAA 2 cut(s) 146, 411
TspRI CASTG 3 cut(s) 433, 457, 481
Tth111I GACNNNGTC 1 cut(s) 482
Vha464I CTTAAG 2 cut(s) 277, 347
XagI CCTNNNNNAGG 1 cut(s) 560
XceI RCATGY 1 cut(s) 19
XspI CTAG 2 cut(s) 32, 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.