Rh4BG326400

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0017693
Physical Location & Seq
Forward (+)
49194507 .. 49195214
708 bp
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UTR
Exon/CDS
Intron
Rh4BG326400.1

Sequence Viewer

Length: 708 bp
ATGCCTAATAATATCAAAGCATCTGTAGATGCTCATCAATCCACACCAGTGGACTATGTTGAAACATTTCAGATTGCGGCTATGCTAAAGATGTGCATGCAATTATTAGGGCTAGTTCGGCATACTGAAAGTGAATCTAAGAGAAACAAGCTGCTCGGAGGAGATGCCAATCAAGCTCCTGCTTCCTTATCGTTGGACTACAGTACACAGTCCAAAAGTATTCTTGTGAGGATAGTTCATGCAGGTGGGCAAGAGGAGCTGTTTCCGAATGCAGTTCCTGCATCTCTGCTGATGGACAAATATCCTGGGAAATGTGTTGCCCGCCCAGAAGTTTTCAAAAAGCCTCAGGAGTCCCTCTTAAGGCCAGACGAGAGCCTATTGCCTGGTCAGAAGTATTATATAATACCATGTACAACAGCACAGAAACTTAAGCGTCAACAGCAGAAGAAGGTGAAGGCCAAAGAACGTGCTGAAGATAATGAGATGATTCTGGATGGAAAGATCACTGTGGATTCGGAAGGGGAAGACACTGATGATTCTATTTGCTCCGCCAGTGACTTCGTCTCCAAGGAAAAGAGACCAAAATCTTCAAGGAGGAGGGGAATTAGAGGAAGAAAGCCGTTTGTACCTCCCCTCCCAAAGGTCAGATCTGGTAGAGGATTTGGATGGGAGCCTAGTCTCACTTCTGTACAAGAAGTGTCTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

235

Amino Acids

26.1

Weight (kDa)

9.28

Isoelectric Point (pI)

60.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 70 - 232 6e-17 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017693)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g24840
malus_domestica MD13G1101500.v1.1
prunus_persica Prupe.1G244300_v2.0.a1
pyrus_communis pycom13g08860 pycom16g08660
rosa_laevigata RLG00000006797
rosa_rugosa Rorug04G0264400
rosa_samantha Rh4AG318300 Rh4BG326400 Rh4CG341700 Rh4DG321600
rosa_wichuraiana Rw4G027670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 233
Acc36I ACCTGC 1 cut(s) 233
AciI CCGC 3 cut(s) 77, 322, 549
AcuI CTGAAG 1 cut(s) 492
AfaI GTAC 4 cut(s) 205, 412, 627, 690
AfiI CCNNNNNNNGG 2 cut(s) 360, 640
AflII CTTAAG 2 cut(s) 358, 428
AgsI TTSAA 3 cut(s) 62, 337, 591
AjnI CCWGG 2 cut(s) 304, 382
AleI CACNNNNGTG 1 cut(s) 47
AluBI AGCT 3 cut(s) 151, 176, 259
AluI AGCT 3 cut(s) 151, 176, 259
Alw26I GTCTC 3 cut(s) 568, 571, 683
AlwNI CAGNNNCTG 1 cut(s) 278
AoxI GGCC 2 cut(s) 362, 456
ApeKI GCWGC 1 cut(s) 151
Asp700I GAANNNNTTC 1 cut(s) 66
AsuHPI GGTGA 1 cut(s) 463
AxyI CCTNAGG 1 cut(s) 345
BbsI GAAGAC 1 cut(s) 531
BbvI GCAGC 1 cut(s) 138
BccI CCATC 3 cut(s) 286, 488, 660
BceAI ACGGC 1 cut(s) 604
BciT130I CCWGG 2 cut(s) 306, 384
BcoDI GTCTC 3 cut(s) 568, 571, 683
BfaI CTAG 2 cut(s) 113, 675
BfmI CTRYAG 2 cut(s) 24, 199
BfrI CTTAAG 2 cut(s) 358, 428
BfuAI ACCTGC 1 cut(s) 233
BglII AGATCT 1 cut(s) 647
BisI GCNGC 2 cut(s) 78, 152
BlsI GCNGC 2 cut(s) 79, 153
Bme1390I CCNGG 2 cut(s) 306, 384
BmiI GGNNCC 1 cut(s) 672
BmrFI CCNGG 2 cut(s) 306, 384
BmsI GCATC 4 cut(s) 19, 29, 154, 290
BpiI GAAGAC 1 cut(s) 531
BsaBI GATNNNNATC 2 cut(s) 33, 168
BsaI GGTCTC 1 cut(s) 571
BsaJI CCNNGG 2 cut(s) 305, 567
BsaXI ACNNNNNCTCC 4 cut(s) 548, 578, 618, 648
Bsc4I CCNNNNNNNGG 2 cut(s) 360, 640
Bse1I ACTGG 2 cut(s) 47, 552
Bse21I CCTNAGG 1 cut(s) 345
Bse8I GATNNNNATC 2 cut(s) 33, 168
BseBI CCWGG 2 cut(s) 306, 384
BseDI CCNNGG 2 cut(s) 305, 567
BseGI GGATG 2 cut(s) 499, 671
BseJI GATNNNNATC 2 cut(s) 33, 168
BseLI CCNNNNNNNGG 2 cut(s) 360, 640
BseMII CTCAG 1 cut(s) 359
BseNI ACTGG 2 cut(s) 47, 552
BseRI GAGGAG 3 cut(s) 174, 269, 610
BseXI GCAGC 1 cut(s) 138
BshFI GGCC 2 cut(s) 364, 458
BslFI GGGAC 1 cut(s) 337
BslI CCNNNNNNNGG 2 cut(s) 360, 640
BsmAI GTCTC 3 cut(s) 568, 571, 683
BsmBI CGTCTC 1 cut(s) 568
BsmFI GGGAC 1 cut(s) 337
BsmI GAATGC 1 cut(s) 274
BsnI GGCC 2 cut(s) 364, 458
Bso31I GGTCTC 1 cut(s) 571
Bsp1407I TGTACA 2 cut(s) 410, 688
Bsp143I GATC 2 cut(s) 501, 647
BspACI CCGC 3 cut(s) 77, 322, 549
BspANI GGCC 2 cut(s) 364, 458
BspCNI CTCAG 1 cut(s) 358
BspLI GGNNCC 1 cut(s) 672
BspMI ACCTGC 1 cut(s) 233
BspTI CTTAAG 2 cut(s) 358, 428
BspTNI GGTCTC 1 cut(s) 571
BsrGI TGTACA 2 cut(s) 410, 688
BsrI ACTGG 2 cut(s) 47, 552
BssECI CCNNGG 2 cut(s) 305, 567
BssMI GATC 2 cut(s) 501, 647
BssT1I CCWWGG 1 cut(s) 567
Bst2UI CCWGG 2 cut(s) 306, 384
Bst4CI ACNGT 3 cut(s) 203, 210, 508
BstAFI CTTAAG 2 cut(s) 358, 428
BstAPI GCANNNNNTGC 1 cut(s) 278
BstAUI TGTACA 2 cut(s) 410, 688
BstC8I GCNNGC 2 cut(s) 98, 322
BstDEI CTNAG 2 cut(s) 138, 345
BstENI CCTNNNNNAGG 1 cut(s) 638
BstF5I GGATG 2 cut(s) 499, 671
BstKTI GATC 2 cut(s) 504, 650
BstMAI GTCTC 3 cut(s) 568, 571, 683
BstMBI GATC 2 cut(s) 501, 647
BstMWI GCNNNNNNNGC 5 cut(s) 118, 173, 256, 278, 439
BstNI CCWGG 2 cut(s) 306, 384
BstNSI RCATGY 1 cut(s) 100
BstSCI CCNGG 2 cut(s) 304, 382
BstSFI CTRYAG 2 cut(s) 24, 199
BstV1I GCAGC 1 cut(s) 138
BstV2I GAAGAC 1 cut(s) 531
BstX2I RGATCY 1 cut(s) 647
BstXI CCANNNNNNTGG 1 cut(s) 49
BstYI RGATCY 1 cut(s) 647
Bsu36I CCTNAGG 1 cut(s) 345
BsuRI GGCC 2 cut(s) 364, 458
BtsCI GGATG 2 cut(s) 499, 671
BtsIMutI CAGTG 4 cut(s) 54, 504, 528, 559
BveI ACCTGC 1 cut(s) 233
Cac8I GCNNGC 2 cut(s) 98, 322
CaiI CAGNNNCTG 1 cut(s) 278
CseI GACGC 1 cut(s) 422
Csp6I GTAC 4 cut(s) 204, 411, 626, 689
CviAII CATG 4 cut(s) 97, 239, 408, 705
CviQI GTAC 4 cut(s) 204, 411, 626, 689
DdeI CTNAG 2 cut(s) 138, 345
DpnI GATC 2 cut(s) 503, 649
DpnII GATC 2 cut(s) 501, 647
EciI GGCGGA 1 cut(s) 538
Eco130I CCWWGG 1 cut(s) 567
Eco31I GGTCTC 1 cut(s) 571
Eco57I CTGAAG 1 cut(s) 492
Eco81I CCTNAGG 1 cut(s) 345
EcoNI CCTNNNNNAGG 1 cut(s) 638
EcoRII CCWGG 2 cut(s) 304, 382
EcoT14I CCWWGG 1 cut(s) 567
ErhI CCWWGG 1 cut(s) 567
Esp3I CGTCTC 1 cut(s) 568
FaeI CATG 4 cut(s) 100, 242, 411, 708
FaiI YATR 9 cut(s) 57, 83, 98, 123, 240, 399, 401, 409, 706
FaqI GGGAC 1 cut(s) 337
FatI CATG 4 cut(s) 96, 238, 407, 704
FauI CCCGC 1 cut(s) 329
Fnu4HI GCNGC 2 cut(s) 78, 152
FokI GGATG 2 cut(s) 506, 678
Fsp4HI GCNGC 2 cut(s) 78, 152
FspBI CTAG 2 cut(s) 113, 675
GluI GCNGC 2 cut(s) 78, 152
HaeIII GGCC 2 cut(s) 364, 458
HgaI GACGC 1 cut(s) 422
Hin1II CATG 4 cut(s) 100, 242, 411, 708
HincII GTYRAC 1 cut(s) 437
HindII GTYRAC 1 cut(s) 437
HinfI GANTC 5 cut(s) 134, 350, 487, 512, 536
HphI GGTGA 1 cut(s) 463
Hpy166II GTNNAC 3 cut(s) 52, 206, 437
Hpy188I TCNGA 6 cut(s) 72, 158, 267, 390, 517, 647
Hpy188III TCNNGA 2 cut(s) 347, 491
Hpy8I GTNNAC 3 cut(s) 52, 206, 437
HpyAV CCTTC 3 cut(s) 442, 448, 512
HpyCH4III ACNGT 3 cut(s) 203, 210, 508
HpyCH4IV ACGT 1 cut(s) 466
HpyCH4V TGCA 5 cut(s) 96, 100, 242, 272, 281
HpyF10VI GCNNNNNNNGC 5 cut(s) 118, 173, 256, 278, 439
HpyF3I CTNAG 2 cut(s) 138, 345
HpySE526I ACGT 1 cut(s) 466
Hsp92II CATG 4 cut(s) 100, 242, 411, 708
Kzo9I GATC 2 cut(s) 501, 647
LmnI GCTCC 4 cut(s) 181, 256, 551, 670
Lsp1109I GCAGC 1 cut(s) 138
LweI GCATC 4 cut(s) 19, 29, 154, 290
MaeI CTAG 2 cut(s) 113, 675
MaeII ACGT 1 cut(s) 466
MaeIII GTNAC 1 cut(s) 554
MalI GATC 2 cut(s) 503, 649
MboI GATC 2 cut(s) 501, 647
MboII GAAGA 5 cut(s) 457, 485, 536, 579, 624
MflI RGATCY 1 cut(s) 647
MluCI AATT 2 cut(s) 101, 603
MlyI GAGTC 1 cut(s) 359
MmeI TCCRAC 1 cut(s) 174
MroXI GAANNNNTTC 1 cut(s) 66
MseI TTAA 2 cut(s) 359, 429
MslI CAYNNNNRTG 2 cut(s) 47, 243
MspCI CTTAAG 2 cut(s) 358, 428
MspR9I CCNGG 2 cut(s) 306, 384
Mva1269I GAATGC 1 cut(s) 274
MvaI CCWGG 2 cut(s) 306, 384
MwoI GCNNNNNNNGC 5 cut(s) 118, 173, 256, 278, 439
NdeII GATC 2 cut(s) 501, 647
NlaIII CATG 4 cut(s) 100, 242, 411, 708
NlaIV GGNNCC 1 cut(s) 672
NmuCI GTSAC 1 cut(s) 554
NspI RCATGY 1 cut(s) 100
OliI CACNNNNGTG 1 cut(s) 47
PaeI GCATGC 1 cut(s) 100
PaqCI CACCTGC 1 cut(s) 233
PctI GAATGC 1 cut(s) 274
PdmI GAANNNNTTC 1 cut(s) 66
PfeI GAWTC 4 cut(s) 134, 487, 512, 536
PflFI GACNNNGTC 1 cut(s) 560
PkrI GCNGC 2 cut(s) 79, 153
PleI GAGTC 1 cut(s) 358
PpsI GAGTC 1 cut(s) 358
Psp6I CCWGG 2 cut(s) 304, 382
PspGI CCWGG 2 cut(s) 304, 382
PspN4I GGNNCC 1 cut(s) 672
PstNI CAGNNNCTG 1 cut(s) 278
PsuI RGATCY 1 cut(s) 647
PsyI GACNNNGTC 1 cut(s) 560
RsaI GTAC 4 cut(s) 205, 412, 627, 690
RsaNI GTAC 4 cut(s) 204, 411, 626, 689
RseI CAYNNNNRTG 2 cut(s) 47, 243
SaqAI TTAA 2 cut(s) 359, 429
SatI GCNGC 2 cut(s) 78, 152
Sau3AI GATC 2 cut(s) 501, 647
SchI GAGTC 1 cut(s) 359
ScrFI CCNGG 2 cut(s) 306, 384
SetI ASST 8 cut(s) 153, 178, 247, 261, 453, 469, 631, 645
SfaNI GCATC 4 cut(s) 19, 29, 154, 290
SfcI CTRYAG 2 cut(s) 24, 199
SmiMI CAYNNNNRTG 2 cut(s) 47, 243
SmlI CTYRAG 2 cut(s) 358, 428
SmoI CTYRAG 2 cut(s) 358, 428
SphI GCATGC 1 cut(s) 100
Sse9I AATT 2 cut(s) 101, 603
SsiI CCGC 3 cut(s) 77, 322, 549
SspMI CTAG 2 cut(s) 113, 675
StyD4I CCNGG 2 cut(s) 304, 382
StyI CCWWGG 1 cut(s) 567
TaaI ACNGT 3 cut(s) 203, 210, 508
TaiI ACGT 1 cut(s) 469
TasI AATT 2 cut(s) 101, 603
TatI WGTACW 3 cut(s) 203, 410, 688
TauI GCSGC 1 cut(s) 80
TfiI GAWTC 4 cut(s) 134, 487, 512, 536
Tru1I TTAA 2 cut(s) 359, 429
Tru9I TTAA 2 cut(s) 359, 429
TscAI CASTG 4 cut(s) 54, 511, 535, 559
TseFI GTSAC 1 cut(s) 554
TseI GCWGC 1 cut(s) 151
Tsp45I GTSAC 1 cut(s) 554
TspDTI ATGAA 1 cut(s) 227
TspRI CASTG 4 cut(s) 54, 511, 535, 559
Tth111I GACNNNGTC 1 cut(s) 560
Vha464I CTTAAG 2 cut(s) 358, 428
XagI CCTNNNNNAGG 1 cut(s) 638
XceI RCATGY 1 cut(s) 100
XmnI GAANNNNTTC 1 cut(s) 66
XspI CTAG 2 cut(s) 113, 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.