pycom16g08660

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0017693
Physical Location & Seq
Reverse (-)
5790123 .. 5790539
417 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g08660.1

Sequence Viewer

Length: 417 bp
ATGCTGAAGCGCTGCATCCAGTTGTTAGGCCTAGCCTGGAATCCTGATAGTGGATCTAAACGAAACAAGCAGTTGACAGGACCTTTCGTGGATGACAGATCGGAAGTAAAGGAAGTAGATGCTGCTTCCATATCATTAGACTTGAGATGGCGCTCGAAGGATGTATTTGTGAGGATAGTTCATGCAGGCGGTCGAGAGGAGCTGTACCAGAATGTAGTTTCTGTATCTCAGTTGATGGAAAAGTATCCTGGTATGTGTGTCGCCCGCCCAGAAGTGTTCAAAAATCCGCAGGAGGCCCTTTTAAGGCCAGAGGAGAGTCTGTTGGTTGGTCAGAAGTATTATATGATACCATCTACGACAGCACGCAAAATTAAGAAAAAAACACCAAAAGAAATTGATGGCCAAAGAAGCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

139

Amino Acids

15.72

Weight (kDa)

9.45

Isoelectric Point (pI)

57.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 55 - 122 8e-13 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017693)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g24840
malus_domestica MD13G1101500.v1.1
prunus_persica Prupe.1G244300_v2.0.a1
pyrus_communis pycom13g08860 pycom16g08660
rosa_laevigata RLG00000006797
rosa_rugosa Rorug04G0264400
rosa_samantha Rh4AG318300 Rh4BG326400 Rh4CG341700 Rh4DG321600
rosa_wichuraiana Rw4G027670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 189, 265, 287
AclWI GGATC 1 cut(s) 61
AcoI YGGCCR 1 cut(s) 400
AcuI CTGAAG 1 cut(s) 26
AfaI GTAC 1 cut(s) 206
AfeI AGCGCT 1 cut(s) 11
AfiI CCNNNNNNNGG 2 cut(s) 50, 303
AgsI TTSAA 1 cut(s) 280
AjnI CCWGG 2 cut(s) 35, 247
AluBI AGCT 2 cut(s) 202, 411
AluI AGCT 2 cut(s) 202, 411
AlwI GGATC 1 cut(s) 61
Aor51HI AGCGCT 1 cut(s) 11
AoxI GGCC 4 cut(s) 28, 294, 305, 400
ApeKI GCWGC 3 cut(s) 12, 122, 411
AspLEI GCGC 2 cut(s) 12, 153
AspS9I GGNCC 2 cut(s) 80, 295
AvaII GGWCC 1 cut(s) 80
BalI TGGCCA 1 cut(s) 402
BbvI GCAGC 2 cut(s) 109, 398
BccI CCATC 4 cut(s) 141, 229, 358, 392
BciT130I CCWGG 2 cut(s) 37, 249
BciVI GTATCC 1 cut(s) 255
BfaI CTAG 1 cut(s) 32
BfoI RGCGCY 2 cut(s) 13, 154
BfuI GTATCC 1 cut(s) 255
BisI GCNGC 3 cut(s) 13, 123, 412
BlsI GCNGC 3 cut(s) 14, 124, 413
Bme1390I CCNGG 2 cut(s) 37, 249
Bme18I GGWCC 1 cut(s) 80
BmgT120I GGNCC 2 cut(s) 80, 295
BmrFI CCNGG 2 cut(s) 37, 249
BmsI GCATC 2 cut(s) 24, 109
BpuEI CTTGAG 1 cut(s) 163
BsaXI ACNNNNNCTCC 2 cut(s) 305, 335
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 303
Bse1I ACTGG 1 cut(s) 19
BseBI CCWGG 2 cut(s) 37, 249
BseGI GGATG 3 cut(s) 15, 97, 166
BseLI CCNNNNNNNGG 2 cut(s) 50, 303
BseMII CTCAG 1 cut(s) 242
BseNI ACTGG 1 cut(s) 19
BseRI GAGGAG 2 cut(s) 212, 326
BseXI GCAGC 2 cut(s) 109, 398
Bsh1285I CGRYCG 1 cut(s) 193
BshFI GGCC 4 cut(s) 30, 296, 307, 402
BsiEI CGRYCG 1 cut(s) 193
BslI CCNNNNNNNGG 2 cut(s) 50, 303
BsnI GGCC 4 cut(s) 30, 296, 307, 402
Bsp143I GATC 2 cut(s) 53, 98
BspACI CCGC 3 cut(s) 189, 265, 287
BspANI GGCC 4 cut(s) 30, 296, 307, 402
BspCNI CTCAG 1 cut(s) 241
BspPI GGATC 1 cut(s) 61
BsrI ACTGG 1 cut(s) 19
BssMI GATC 2 cut(s) 53, 98
Bst2UI CCWGG 2 cut(s) 37, 249
BstC8I GCNNGC 3 cut(s) 187, 265, 364
BstDEI CTNAG 1 cut(s) 228
BstF5I GGATG 3 cut(s) 15, 97, 166
BstH2I RGCGCY 2 cut(s) 13, 154
BstHHI GCGC 2 cut(s) 12, 153
BstKTI GATC 2 cut(s) 56, 101
BstMBI GATC 2 cut(s) 53, 98
BstMCI CGRYCG 1 cut(s) 193
BstMWI GCNNNNNNNGC 1 cut(s) 408
BstNI CCWGG 2 cut(s) 37, 249
BstSCI CCNGG 2 cut(s) 35, 247
BstV1I GCAGC 2 cut(s) 109, 398
BstX2I RGATCY 1 cut(s) 53
BstYI RGATCY 1 cut(s) 53
BsuI GTATCC 1 cut(s) 255
BsuRI GGCC 4 cut(s) 30, 296, 307, 402
BtsCI GGATG 3 cut(s) 15, 97, 166
Cac8I GCNNGC 3 cut(s) 187, 265, 364
CfoI GCGC 2 cut(s) 12, 153
Cfr13I GGNCC 2 cut(s) 80, 295
Csp6I GTAC 1 cut(s) 205
CviAII CATG 1 cut(s) 182
CviJI RGCY 7 cut(s) 30, 35, 202, 296, 307, 402, 411
CviKI_1 RGCY 7 cut(s) 30, 35, 202, 296, 307, 402, 411
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 1 cut(s) 228
DpnI GATC 2 cut(s) 55, 100
DpnII GATC 2 cut(s) 53, 98
EaeI YGGCCR 1 cut(s) 400
Eco147I AGGCCT 1 cut(s) 30
Eco47I GGWCC 1 cut(s) 80
Eco47III AGCGCT 1 cut(s) 11
Eco57I CTGAAG 1 cut(s) 26
EcoO109I RGGNCCY 2 cut(s) 80, 295
EcoRII CCWGG 2 cut(s) 35, 247
FaeI CATG 1 cut(s) 185
FaiI YATR 5 cut(s) 131, 183, 254, 342, 344
FatI CATG 1 cut(s) 181
FauI CCCGC 1 cut(s) 272
Fnu4HI GCNGC 3 cut(s) 13, 123, 412
FokI GGATG 3 cut(s) 2, 104, 173
Fsp4HI GCNGC 3 cut(s) 13, 123, 412
FspBI CTAG 1 cut(s) 32
GlaI GCGC 2 cut(s) 11, 152
GluI GCNGC 3 cut(s) 13, 123, 412
HaeII RGCGCY 2 cut(s) 13, 154
HaeIII GGCC 4 cut(s) 30, 296, 307, 402
HhaI GCGC 2 cut(s) 12, 153
Hin1II CATG 1 cut(s) 185
Hin6I GCGC 2 cut(s) 10, 151
HinP1I GCGC 2 cut(s) 10, 151
HincII GTYRAC 1 cut(s) 75
HindII GTYRAC 1 cut(s) 75
HinfI GANTC 2 cut(s) 40, 316
Hpy166II GTNNAC 1 cut(s) 75
Hpy188I TCNGA 2 cut(s) 103, 333
Hpy188III TCNNGA 2 cut(s) 44, 194
Hpy8I GTNNAC 1 cut(s) 75
HpyAV CCTTC 1 cut(s) 151
HpyCH4V TGCA 2 cut(s) 15, 185
HpyF10VI GCNNNNNNNGC 1 cut(s) 408
HpyF3I CTNAG 1 cut(s) 228
Hsp92II CATG 1 cut(s) 185
HspAI GCGC 2 cut(s) 10, 151
Kzo9I GATC 2 cut(s) 53, 98
LmnI GCTCC 1 cut(s) 199
Lsp1109I GCAGC 2 cut(s) 109, 398
LweI GCATC 2 cut(s) 24, 109
MaeI CTAG 1 cut(s) 32
MalI GATC 2 cut(s) 55, 100
MboI GATC 2 cut(s) 53, 98
MflI RGATCY 1 cut(s) 53
MlsI TGGCCA 1 cut(s) 402
MluCI AATT 2 cut(s) 369, 393
MluNI TGGCCA 1 cut(s) 402
MlyI GAGTC 1 cut(s) 325
MnlI CCTC 4 cut(s) 165, 190, 286, 304
Mox20I TGGCCA 1 cut(s) 402
MscI TGGCCA 1 cut(s) 402
MseI TTAA 2 cut(s) 302, 372
Msp20I TGGCCA 1 cut(s) 402
MspR9I CCNGG 2 cut(s) 37, 249
MvaI CCWGG 2 cut(s) 37, 249
MwoI GCNNNNNNNGC 1 cut(s) 408
NdeII GATC 2 cut(s) 53, 98
NlaIII CATG 1 cut(s) 185
PceI AGGCCT 1 cut(s) 30
PfeI GAWTC 1 cut(s) 40
PkrI GCNGC 3 cut(s) 14, 124, 413
PleI GAGTC 1 cut(s) 324
PpsI GAGTC 1 cut(s) 324
PpuMI RGGWCCY 1 cut(s) 80
Psp5II RGGWCCY 1 cut(s) 80
Psp6I CCWGG 2 cut(s) 35, 247
PspGI CCWGG 2 cut(s) 35, 247
PspPI GGNCC 2 cut(s) 80, 295
PspPPI RGGWCCY 1 cut(s) 80
PsuI RGATCY 1 cut(s) 53
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
SaqAI TTAA 2 cut(s) 302, 372
SatI GCNGC 3 cut(s) 13, 123, 412
Sau3AI GATC 2 cut(s) 53, 98
Sau96I GGNCC 2 cut(s) 80, 295
SchI GAGTC 1 cut(s) 325
ScrFI CCNGG 2 cut(s) 37, 249
SetI ASST 3 cut(s) 85, 204, 413
SfaNI GCATC 2 cut(s) 24, 109
SinI GGWCC 1 cut(s) 80
SmlI CTYRAG 1 cut(s) 142
SmoI CTYRAG 1 cut(s) 142
Sse9I AATT 2 cut(s) 369, 393
SseBI AGGCCT 1 cut(s) 30
SsiI CCGC 3 cut(s) 189, 265, 287
SspMI CTAG 1 cut(s) 32
StuI AGGCCT 1 cut(s) 30
StyD4I CCNGG 2 cut(s) 35, 247
TaqI TCGA 2 cut(s) 155, 193
TasI AATT 2 cut(s) 369, 393
TfiI GAWTC 1 cut(s) 40
Tru1I TTAA 2 cut(s) 302, 372
Tru9I TTAA 2 cut(s) 302, 372
TseI GCWGC 3 cut(s) 12, 122, 411
TspDTI ATGAA 1 cut(s) 170
VpaK11BI GGWCC 1 cut(s) 80
XspI CTAG 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.