Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0014998

Physical Location & Seq

Genomic Coordinates Forward (+)

20049321 .. 20056090

Gene Structure 6770 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g26200.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5277 bp

ATGAGTATCCGGCCTCCGGACCCGGATTCCGAGCCCTACAACATCATCCCCGTCCACAACCTCCTCGCGGACCACCCCTCTCTCCGGTTCCCTGAGGTCCGCGCCGCCGCCGCGTCCCTCCGCGCCGTGGGCAACCTCCGCAGGCCGCCCTACGCCCAATGGCGGCCCCACATGGACCTCCTGGACTGGCTGGCCTTGTTCTTCGGGTTCCAATACGACAACGTCCGCAACCAGAGGGAGCACATCGTCCTCCACTTGGCCAACGCCCAGATGCGCCTCAGCCCTCCCCCTGACAACATCGACACTCTGGACGGCGGCGTCCTCCGCAAGTTCCGCAAGAAGCTCCTCCTCAATTACACCAACTGGTGCTCCTACCTGGGCAAGAAATCCAACATCTGGATCTCCGCCTCCTCCGACCACCGCCGCGAGCTCCTCTACGTCTCCCTCTACCTCCTCATCTGGGGCGAGGCCGCCAATCTCAGGTTCGTGCCCGAGTGCTTGTGCTATATCTTTCATAACATGGCCATGGAGTTGAATAAGATTCTGGAGGATTACATTGATGAGAGCACCGGGCAGCCTGTTATGCCCTCCGTTTCCGGGGAGAATGCCTTCCTGAATTGCGTCGTCAAGCCCATTTACGACACCATCCGGGCCGAGGTTGAGGGCAGCAAGAATGGGACTGCCCCCCACAGTGTTTGGAGGAACTATGATGATATCAATGAGTACTTCTGGAGCAAGCGCTGTTTCGACAAGCTCAAGTGGCCTGTCGACGTCGGCAGCAACTTCTTTGTTACTAACACCAAGAGTAAGCATGTGGGCAAGACCGGCTTTGTGGAGCAGAGGTCCTTCTGGAACTTGTTTAGGAGCTTTGACAAGCTTTGGATCATGCTGCTCTTGTTCCTCCAGGCCGCCATCATAGTTGCTTGGGAGGAGAGGGAGTATCCCTGGCAGGCCTTGCAGGAGAGGCAAGTCCAGGTCAAGGTTCTCACTGTGTTTTTCACTTGGAGTGGTTTGAGGTTTCTGCAGTCCCTACTTGATGTGGGGATGCAGTATAGTCTGGTTTCCAGGGAGACTTTGGGGCTCGGGGTCAGGATGGTCTTCAAGAGCATTGCTGCTGCAGGCTGGATTGTTGTTTTCGGGGTCTTTTATGGGAGGATATGGTCCCAGAGAAACCTGGATAAGCGGTGGTCTCCTGAGGCAGACAGCCGGGTTGTGCAGTTTCTTTTGGTGTCGTTGGTTTTCATCATTCCAGAGCTCTTGGCCATAACCTTCTTTATTCTTCCTTGGATTCGCAATTTTATGGAGAACTCAAACTGGAGGATATTTTATGCCTTGTCCTGGTGGTTTCAGAGCAAAACTTTTGTCGGCCGTGGCTTGAGGGAAGGCCTTGTGGACAATGTCAAGTACACTTTGTTCTGGATTTTGGTGCTATCTACCAAGTTTGCTTTCAGTTACTTCATGCTGATTAAACCCATGATTGTTCCATCTAAAGCACTCGTAAAACTTGATAATGTGGAGTATGAGTGGTTTCAGATTCTCAAAAACAGCAACAAAATGGCGGTGGGATTGTTGTGGCTTCCTGTCGTTTTGATATACCTCATGGACATGCAGATCTGGTATTCAATCTACTCCTCATTTTGGGGGGCATTAGTCGGTTTGCTTGCGCATTTGGGTGAGATTCGAAATATCCAACAGTTGAGGCTGAGGTTCCAGTTCTTTGCAAGTGCTATTCAGTTTAATCTCATGCCAGAGGAGCAGATGTTAAATGCAAGGGGGACACTGAGAAGCAAGTTTAATGACGCTATCCACAGGTTGAAGCTGAGGTATGGGCTTGGACGGCCTTATAAGAAGCTTGAATCCAACCAGATCGAGGCAACCAAGTTTGCTTTGATATGGAATGAGATCATATTGATCTTCAGGGAAGAGGATTTGATTTCGGACAGCGAGGTTGAGTTGTTAGAGCTACCACAGAATTCTTGGAATGTCAGGGTCATTCGCTGGCCTTGTTTTCTTTTGTGCAATGAACTGCTGCTTGCACTTAGTCAGGCCAAAGAGTTGGTAGACGCGCCTGACAAGTGGCTCTGGTATAAGATATGCAAGAATGAGTACAGGCGCTGTGCTGTGATAGAAGCTTATGATTGTGTCAAGCACATGATACTTGCCATTATAAAACCCAACACAGAAGAGCATTCAATCGTGACAGTCTTGTTTCAGGAAATTGATCACTCTATTCAGATTGAGAAGTTCACAAAAACTTTTAAAACCGCTGCCCTTCCACTACTCCATGCCAAGTTGATCAAGCTTTCTGAGCTATTGAACAAACCTAAGAAAGATACAAACCAGGTGGTGAATACCCTTCAGGCTCTTTATGAGATTGCCATTCGAGATTTCTTCAAGGAGAAAAGAAGTACTGAACAGCTTTTGGAGGACGGTTTGGCTCTCCGTGATCCATCTTCTGCTGCAGGGCTGCTTTTTGAAAATGCTGTTGGGTTGCCTGATCCTAGTGATGGATCCTTCTATCGGCAGGTTCGGCGCCTGCACACAATACTTACCTCACGGGACTCGATGCAGAATATTCCAGTTAACCTTGAGGCGAGACGCAGAATTGCCTTCTTTAGTAACTCCCTGTTTATGAACATACCCCATGCTCCACAAGTTGAGAAAATGATGGCTTTCAGTGTTCTGACCCCATATTACAGTGAAGAAGTATTGTACAGCAAAGAACAACTGCGTACTGAGAATGAAGATGGGATTTCTACCCTGTACTATCTACAAACAATTTATGTTGATGAGTGGAAAAATTTCATGGAGAGAATGCGTCGAGAAGGAATAGCAAATGATGATGAGATATGGACAACGAAGTTGAGGGAGCTCAGGCTTTGGGCATCTTACAGAGGACAGACACTTACTCGAACCGTAAGGGGTATGATGTATTATTTTCGGGCTCTTAAGATGTTGGCTTTTCTGGATTCTGCATCAGAGATGGACATTCGGGAAGGTTCACAAGAACTTGGTTCTATGATGCGAGACATTGGTTTGGATGGTTTGACCTTGGAGAAGTCACTTTCTTCCAGGAGTTTAAGTAGGACAAGCAGTTGTGTGAACTCATTGTACAAAGGTCATGAAGTTGGAACTGCTTTGATGAAATATACATATGTGGTTGCATGCCAGATATATGGAACACAAAAGGCGAAGAAAGATCCCCATGCTGATGAAATTTTGTATCTGATGAAAACCAATGAAGCACTTCGAATTGCCTATGTTGACGAGGTTTCAACTGGCAGGGATGAGAAGGAATATTATTCTGTTCTCGTCAAGTATGATAATCAGTTAGAGAAGGAAGTGGAAATCTATCGCATTAAGTTGCCTGGTCCCTTGAAGCTCGGAGAGGGGAAACCAGAGAATCAAAATCATGCCATTATCTTCACTCGTGGTGATGCAGTTCAGACTATTGATATGAACCAGGACAATTATTTTGAGGAGGCACTTAAAATGCGGAATCTGTTGGAAGAATTCAGGCGCTATTATGGTATCCGAAAGCCTACGATCTTGGGAGTTAGGGAGCATGTCTTTACTGGTTCTGTCTCATCTCTTGCTTGGTTTATGTCAGCTCAGGAAACAAGTTTTGTCACCTTGGGTCAGCGGGTGTTGGCAAACCCTTTGAAAATTCGAATGCATTATGGTCATCCAGATGTCTTTGACCGATTTTGGTTCTTGACTCGAGGTGGCATCAGTAAAGCTTCCAGGGTGATTAACATCAGTGAAGACATTTTTGCTGGCTTTAATTGCACTTTGCGTGGAGGCAACGTCACCCACCATGAATACATCCAAGTTGGCAAGGGAAGGGATGTTGGATTTAACCAAATCTCCATGTTTGAGGCCAAGGTTGCTAGTGGAAATGGGGAGCAAGTTCTGAGCAGAGATGTGTATAGGTTAGGCCATAGGCTTGATTTCTTGCGGATGCTGTCATTCTTTTATACTACTGTGGGTTTCTTTTTCAACACAATGATGGTGATTTTAACTGTGTATGCATTTCTATGGGGCCGACTTTATTTGGCTCTCAGTGGTATTGAAGGTTCTATATTGGGGGATGATACTAGTAATAGAGCACTTGGTACAGTCTTGAATCAACAGTTTATTATCCAGCTTGGTCTGTTCACTGCCCTTCCAATGATAGTAGAGAATTCTCTCGAGCATGGGTTTCTCCAAGCTATCTGGGATTTCCTGACAATGCAGCTCCAGCTTTCTTCAGTTTTTTACACATTTTCGATGGGAACTCGTACCCACTATTTTGGGAGAACCATTCTTCATGGTGGGGCAAAATATCGAGCGACGGGACGTGGCTTTGTTGTGCAGCACAAGAGTTTTGCAGAGAATTATAGGCTCTATGCCCGTAGCCATTTTGTGAAAGCAATTGAACTCGGTTTGATACTTACAGTTTATGCAGCATACAGTCCTGTGGCTAAGGACACATTTGTTTACATAGCAATGACCATCACAAGTTGGTTCATGGTTTTGTCCTGGTTTATGGCTCCCTTCGTTTTCAATCCTTCTGGCTTTGACTGGTTGAAGACTGTTGATGATTTTGATGACTTTATGAACTGGATTTGGTATCGTGGTAGTGTGTTTGCCAAAGCTGAACAAAGCTGGGAAAGATGGTGGTATGAGGAACAGGATCATCTAAGGACAACCGGTGTGTGGGGAAAGTTGCTGGAGATAATTTTAGACCTTCGCTTCTTCTTTTTCCAGTATGGGATCGTGTACCAGCTTGGTATTGCTGATAACAGTAAAAGTATACTTGTTTACTTGTTATCTTGGATCTATGTGTTCCTGGCTTTTGGCATATTTATCGTGATAGTGTATGCTCGGGTAAAATATGCAGCAAAAGATCACATTTACTACCGGCTGGTCCAATTTCTTGTCATTAAACTTGCACTACTGGTAATAATTGCCCTGTTGGAATTCACAAACTTCAAGTTCATGGATATATTCACTAGTCTTCTGGCATTCATCCCCACTGGTTGGGGTTTGATATTGATAGCACAAGTATTCCGGCCCTTACTACAACGCACTATACTATGGGAGGTTGTTGTTTCTGTGGCTCGACTGTACGATATCTTGTTCGGGGTGATTGTATTGACTCCTGTGGCAGTATTATCATGGTTTCCTGGGTTCCAGTCTATGCAGACTAGGATACTGTTTAATGATGCATTCAGCAGGGGACTTCGTATATTCCAGATTGTTACAGGAAAAAAGAAGTCCAAGAGAGATTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0005911 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0009506 GO:0009555 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009653 GO:0009719 GO:0009725 GO:0009751 GO:0009863 GO:0009870 GO:0009965 GO:0009987 GO:0010016 GO:0010033 GO:0010150 GO:0012505 GO:0014070 GO:0016020 GO:0016043 GO:0023052 GO:0030054 GO:0031347 GO:0031349 GO:0032501 GO:0032502 GO:0032870 GO:0033036 GO:0033037 GO:0033554 GO:0035690 GO:0042221 GO:0042493 GO:0042545 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045089 GO:0045229 GO:0046677 GO:0048229 GO:0048366 GO:0048367 GO:0048518 GO:0048583 GO:0048584 GO:0048731 GO:0048827 GO:0048856 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051179 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0055044 GO:0065007 GO:0070727 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071407 GO:0071446 GO:0071495 GO:0071554 GO:0071555 GO:0071840 GO:0071944 GO:0080134 GO:0090693 GO:0098542 GO:0099402 GO:1901700 GO:1901701 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FKS1_dom1 Glucan_synthase

Protein Analysis

1759

Amino Acids

204.48

Weight (kDa)

8.99

Isoelectric Point (pI)

37.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FKS1_dom1	PF14288	143 - 255	1.4e-44	1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1	PF25968	668 - 851	2.7e-31	Callose synthase 1 domain
Glucan_synthase	PF02364	859 - 949	2.1e-30	1,3-beta-glucan synthase component
Glucan_synthase	PF02364	955 - 1646	4.9e-233	1,3-beta-glucan synthase component

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0014998)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G03550
fragaria_vesca	FvH4_6g26200
malus_domestica	MD09G1247400.v1.1 MD17G1240300.v1.1
prunus_persica	Prupe.3G138400_v2.0.a1
pyrus_communis	pycom17g24360
rosa_chinensis	RchiOBHm_Chr2g0126481
rosa_laevigata	RLG00000018906
rosa_multiflora	Rmu_sc0000662.1_g000007
rosa_roxburghii	Rroxscaffold_2G00117140
rosa_rugosa	Rorug02G0263500
rosa_samantha	Rh2AG320100 Rh2BG328800 Rh2CG308500 Rh2DG348100
rosa_wichuraiana	Rw2G025950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1845, 2168
AasI	GACNNNNNNGTC	1 cut(s)	317
AatII	GACGTC	1 cut(s)	774
Acc16I	TGCGCA	1 cut(s)	1665
Acc36I	ACCTGC	1 cut(s)	2515
AccB1I	GGYRCC	1 cut(s)	2532
AccB7I	CCANNNNNTGG	2 cut(s)	396, 5022
AccI	GTMKAC	3 cut(s)	768, 2061, 4795
AccII	CGCG	6 cut(s)	68, 102, 113, 123, 426, 2066
AccIII	TCCGGA	1 cut(s)	16
AcoI	YGGCCR	4 cut(s)	258, 522, 1260, 1366
AcsI	RAATTY	7 cut(s)	1972, 2800, 3216, 3512, 3666, 4183, 4961
AcuI	CTGAAG	3 cut(s)	1900, 2342, 4233
AcyI	GRCGYC	3 cut(s)	318, 771, 2533
AfeI	AGCGCT	1 cut(s)	740
AflII	CTTAAG	1 cut(s)	2948
AgeI	ACCGGT	1 cut(s)	4691
AhlI	ACTAGT	2 cut(s)	4097, 4994
AjiI	CACGTC	1 cut(s)	4340
AjuI	GAANNNNNNNTTGG	6 cut(s)	467, 499, 1430, 1462, 2469, 2501
Alw21I	GWGCWC	7 cut(s)	243, 371, 432, 569, 1257, 2874, 4111
Alw26I	GTCTC	6 cut(s)	445, 1064, 1194, 2590, 3021, 3589
AlwNI	CAGNNNCTG	1 cut(s)	2115
Ama87I	CYCGRG	5 cut(s)	491, 1082, 3720, 4190, 4866
Aor13HI	TCCGGA	1 cut(s)	16
Aor51HI	AGCGCT	1 cut(s)	740
ApoI	RAATTY	7 cut(s)	1972, 2800, 3216, 3512, 3666, 4183, 4961
ArsI	GACNNNNNNTTYG	4 cut(s)	1347, 1379, 3458, 3490
AsiGI	ACCGGT	1 cut(s)	4691
Asp700I	GAANNNNTTC	3 cut(s)	608, 2801, 3246
AspLEI	GCGC	9 cut(s)	104, 125, 276, 741, 1666, 2068, 2115, 2535, 3522
AsuC2I	CCSGG	5 cut(s)	23, 571, 598, 650, 1208
AsuHPI	GGTGA	8 cut(s)	1685, 2359, 3446, 3622, 3760, 3802, 4024, 5140
AsuII	TTCGAA	3 cut(s)	1681, 3250, 3670
AvaI	CYCGRG	5 cut(s)	491, 1082, 3720, 4190, 4866
AvaII	GGWCC	8 cut(s)	19, 70, 97, 175, 843, 1161, 3371, 4909
AxyI	CCTNAGG	2 cut(s)	93, 1194
BaeGI	GKGCMC	1 cut(s)	492
BalI	TGGCCA	3 cut(s)	260, 524, 1262
BamHI	GGATCC	1 cut(s)	2510
BanI	GGYRCC	1 cut(s)	2532
BanII	GRGCYC	6 cut(s)	36, 432, 1083, 1257, 2874, 2947
BauI	CACGAG	1 cut(s)	3429
BbsI	GAAGAC	4 cut(s)	1090, 3771, 4577, 4991
Bbv12I	GWGCWC	7 cut(s)	243, 371, 432, 569, 1257, 2874, 4111
BbvCI	CCTCAGC	3 cut(s)	278, 1703, 1820
BceAI	ACGGC	4 cut(s)	110, 328, 1353, 1853
BcgI	CGANNNNNNTGC	2 cut(s)	4831, 4865
BciVI	GTATCC	4 cut(s)	17, 951, 3542, 5187
BclI	TGATCA	2 cut(s)	2221, 2295
BcnI	CCSGG	5 cut(s)	23, 571, 598, 650, 1208
BcoDI	GTCTC	6 cut(s)	445, 1064, 1194, 2590, 3021, 3589
BcuI	ACTAGT	2 cut(s)	4097, 4994
BfaI	CTAG	5 cut(s)	2502, 3891, 4098, 4995, 5190
BfmI	CTRYAG	3 cut(s)	1022, 1116, 2460
BfoI	RGCGCY	4 cut(s)	742, 2116, 2536, 3523
BfrI	CTTAAG	1 cut(s)	2948
BfuAI	ACCTGC	1 cut(s)	2515
BfuI	GTATCC	4 cut(s)	17, 951, 3542, 5187
BglII	AGATCT	1 cut(s)	1611
BmcAI	AGTACT	2 cut(s)	725, 2410
Bme18I	GGWCC	8 cut(s)	19, 70, 97, 175, 843, 1161, 3371, 4909
BmeT110I	CYCGRG	5 cut(s)	491, 1082, 3720, 4190, 4866
BmgBI	CACGTC	1 cut(s)	4340
BpiI	GAAGAC	4 cut(s)	1090, 3771, 4577, 4991
BpmI	CTGGAG	6 cut(s)	566, 751, 887, 1336, 4223, 4733
Bpu10I	CCTNAGC	6 cut(s)	278, 1703, 1820, 2873, 3612, 4464
Bpu14I	TTCGAA	3 cut(s)	1681, 3250, 3670
BpuEI	CTTGAG	3 cut(s)	740, 1396, 2609
BpuMI	CCSGG	5 cut(s)	23, 571, 598, 650, 1208
BsaBI	GATNNNNATC	1 cut(s)	3755
BsaHI	GRCGYC	3 cut(s)	318, 771, 2533
BsaI	GGTCTC	1 cut(s)	1194
BsaWI	WCCGGW	3 cut(s)	16, 84, 4691
Bse118I	RCCGGY	3 cut(s)	824, 4691, 4902
Bse21I	CCTNAGG	2 cut(s)	93, 1194
Bse3DI	GCAATG	3 cut(s)	1107, 2026, 4494
Bse8I	GATNNNNATC	1 cut(s)	3755
BseAI	TCCGGA	1 cut(s)	16
BseJI	GATNNNNATC	1 cut(s)	3755
BseMI	GCAATG	3 cut(s)	1107, 2026, 4494
BseSI	GKGCMC	1 cut(s)	492
BseX3I	CGGCCG	1 cut(s)	1366
BseYI	CCCAGC	1 cut(s)	4647
BsgI	GTGCAG	3 cut(s)	1235, 2522, 4373
Bsh1236I	CGCG	6 cut(s)	68, 102, 113, 123, 426, 2066
Bsh1285I	CGRYCG	1 cut(s)	1369
BshNI	GGYRCC	1 cut(s)	2532
BshTI	ACCGGT	1 cut(s)	4691
BsiEI	CGRYCG	1 cut(s)	1369
BsiHKAI	GWGCWC	7 cut(s)	243, 371, 432, 569, 1257, 2874, 4111
BsiHKCI	CYCGRG	5 cut(s)	491, 1082, 3720, 4190, 4866
BslFI	GGGAC	9 cut(s)	100, 691, 1012, 1147, 1789, 2573, 3357, 4350, 5235
BsmAI	GTCTC	6 cut(s)	445, 1064, 1194, 2590, 3021, 3589
BsmBI	CGTCTC	2 cut(s)	445, 2590
BsmFI	GGGAC	9 cut(s)	100, 691, 1012, 1147, 1789, 2573, 3357, 4350, 5235
BsmI	GAATGC	6 cut(s)	610, 2188, 2820, 3678, 5006, 5210
Bso31I	GGTCTC	1 cut(s)	1194
BsoBI	CYCGRG	5 cut(s)	491, 1082, 3720, 4190, 4866
Bsp119I	TTCGAA	3 cut(s)	1681, 3250, 3670
Bsp13I	TCCGGA	1 cut(s)	16
Bsp1407I	TGTACA	2 cut(s)	2712, 3111
Bsp19I	CCATGG	1 cut(s)	525
BspEI	TCCGGA	1 cut(s)	16
BspFNI	CGCG	6 cut(s)	68, 102, 113, 123, 426, 2066
BspHI	TCATGA	1 cut(s)	3121
BspMAI	CTGCAG	3 cut(s)	1026, 1120, 2464
BspMI	ACCTGC	1 cut(s)	2515
BspQI	GCTCTTC	1 cut(s)	2178
BspT104I	TTCGAA	3 cut(s)	1681, 3250, 3670
BspT107I	GGYRCC	1 cut(s)	2532
BspTI	CTTAAG	1 cut(s)	2948
BspTNI	GGTCTC	1 cut(s)	1194
BsrDI	GCAATG	3 cut(s)	1107, 2026, 4494
BsrFI	RCCGGY	3 cut(s)	824, 4691, 4902
BsrGI	TGTACA	2 cut(s)	2712, 3111
BssAI	RCCGGY	3 cut(s)	824, 4691, 4902
BssNAI	GTATAC	1 cut(s)	4796
BssNI	GRCGYC	3 cut(s)	318, 771, 2533
BssSI	CACGAG	1 cut(s)	3429
BssT1I	CCWWGG	5 cut(s)	525, 1283, 3051, 3633, 3882
Bst1107I	GTATAC	1 cut(s)	4796
Bst2BI	CACGAG	1 cut(s)	3429
Bst6I	CTCTTC	2 cut(s)	1917, 2178
BstACI	GRCGYC	3 cut(s)	318, 771, 2533
BstAFI	CTTAAG	1 cut(s)	2948
BstAPI	GCANNNNNTGC	1 cut(s)	955
BstAUI	TGTACA	2 cut(s)	2712, 3111
BstBI	TTCGAA	3 cut(s)	1681, 3250, 3670
BstDSI	CCRYGG	3 cut(s)	126, 525, 1369
BstFNI	CGCG	6 cut(s)	68, 102, 113, 123, 426, 2066
BstH2I	RGCGCY	4 cut(s)	742, 2116, 2536, 3523
BstHHI	GCGC	9 cut(s)	104, 125, 276, 741, 1666, 2068, 2115, 2535, 3522
BstMAI	GTCTC	6 cut(s)	445, 1064, 1194, 2590, 3021, 3589
BstMCI	CGRYCG	1 cut(s)	1369
BstNSI	RCATGY	4 cut(s)	815, 1609, 3168, 3569
BstSFI	CTRYAG	3 cut(s)	1022, 1116, 2460
BstSLI	GKGCMC	1 cut(s)	492
BstUI	CGCG	6 cut(s)	68, 102, 113, 123, 426, 2066
BstV2I	GAAGAC	4 cut(s)	1090, 3771, 4577, 4991
BstX2I	RGATCY	5 cut(s)	399, 1611, 2510, 3199, 4818
BstXI	CCANNNNNNTGG	3 cut(s)	2056, 3176, 4292
BstYI	RGATCY	5 cut(s)	399, 1611, 2510, 3199, 4818
BstZ17I	GTATAC	1 cut(s)	4796
BstZI	CGGCCG	1 cut(s)	1366
Bsu36I	CCTNAGG	2 cut(s)	93, 1194
BsuI	GTATCC	4 cut(s)	17, 951, 3542, 5187
BtgI	CCRYGG	3 cut(s)	126, 525, 1369
BtrI	CACGTC	1 cut(s)	4340
BtsI	GCAGTG	1 cut(s)	4158
BtsIMutI	CAGTG	9 cut(s)	697, 987, 1778, 2683, 2704, 3766, 4069, 4158, 5016
BveI	ACCTGC	1 cut(s)	2515
CaiI	CAGNNNCTG	1 cut(s)	2115
CciI	TCATGA	1 cut(s)	3121
CfoI	GCGC	9 cut(s)	104, 125, 276, 741, 1666, 2068, 2115, 2535, 3522
Cfr10I	RCCGGY	3 cut(s)	824, 4691, 4902
CseI	GACGC	7 cut(s)	102, 307, 610, 1808, 2072, 2607, 2807
CsiI	ACCWGGT	1 cut(s)	2340
CspAI	ACCGGT	1 cut(s)	4691
CspCI	CAANNNNNGTGG	4 cut(s)	677, 712, 2325, 2360
DinI	GGCGCC	1 cut(s)	2534
DraI	TTTAAA	1 cut(s)	2260
DrdI	GACNNNNNNGTC	1 cut(s)	317
DseDI	GACNNNNNNGTC	1 cut(s)	317
EaeI	YGGCCR	4 cut(s)	258, 522, 1260, 1366
EagI	CGGCCG	1 cut(s)	1366
Eam1104I	CTCTTC	2 cut(s)	1917, 2178
EarI	CTCTTC	2 cut(s)	1917, 2178
EciI	GGCGGA	1 cut(s)	394
Ecl136II	GAGCTC	3 cut(s)	430, 1255, 2872
EclXI	CGGCCG	1 cut(s)	1366
Eco130I	CCWWGG	5 cut(s)	525, 1283, 3051, 3633, 3882
Eco147I	AGGCCT	2 cut(s)	953, 1386
Eco24I	GRGCYC	6 cut(s)	36, 432, 1083, 1257, 2874, 2947
Eco31I	GGTCTC	1 cut(s)	1194
Eco32I	GATATC	2 cut(s)	715, 5116
Eco47I	GGWCC	8 cut(s)	19, 70, 97, 175, 843, 1161, 3371, 4909
Eco47III	AGCGCT	1 cut(s)	740
Eco52I	CGGCCG	1 cut(s)	1366
Eco53kI	GAGCTC	3 cut(s)	430, 1255, 2872
Eco57I	CTGAAG	3 cut(s)	1900, 2342, 4233
Eco81I	CCTNAGG	2 cut(s)	93, 1194
Eco88I	CYCGRG	5 cut(s)	491, 1082, 3720, 4190, 4866
EcoICRI	GAGCTC	3 cut(s)	430, 1255, 2872
EcoO109I	RGGNCCY	1 cut(s)	843
EcoRI	GAATTC	4 cut(s)	1972, 3512, 4183, 4961
EcoRV	GATATC	2 cut(s)	715, 5116
EcoT14I	CCWWGG	5 cut(s)	525, 1283, 3051, 3633, 3882
EcoT22I	ATGCAT	3 cut(s)	3678, 4033, 5212
EcoT38I	GRGCYC	6 cut(s)	36, 432, 1083, 1257, 2874, 2947
EgeI	GGCGCC	1 cut(s)	2534
EheI	GGCGCC	1 cut(s)	2534
ErhI	CCWWGG	5 cut(s)	525, 1283, 3051, 3633, 3882
Esp3I	CGTCTC	2 cut(s)	445, 2590
FalI	AAGNNNNNCTT	2 cut(s)	812, 844
FaqI	GGGAC	9 cut(s)	100, 691, 1012, 1147, 1789, 2573, 3357, 4350, 5235
FauI	CCCGC	1 cut(s)	3636
FauNDI	CATATG	1 cut(s)	3154
FbaI	TGATCA	2 cut(s)	2221, 2295
FblI	GTMKAC	3 cut(s)	768, 2061, 4795
FriOI	GRGCYC	6 cut(s)	36, 432, 1083, 1257, 2874, 2947
FspBI	CTAG	5 cut(s)	2502, 3891, 4098, 4995, 5190
FspI	TGCGCA	1 cut(s)	1665
GlaI	GCGC	9 cut(s)	103, 124, 275, 740, 1665, 2067, 2114, 2534, 3521
GsaI	CCCAGC	1 cut(s)	4651
GsuI	CTGGAG	6 cut(s)	566, 751, 887, 1336, 4223, 4733
HaeII	RGCGCY	4 cut(s)	742, 2116, 2536, 3523
HgaI	GACGC	7 cut(s)	102, 307, 610, 1808, 2072, 2607, 2807
HhaI	GCGC	9 cut(s)	104, 125, 276, 741, 1666, 2068, 2115, 2535, 3522
Hin1I	GRCGYC	3 cut(s)	318, 771, 2533
Hin6I	GCGC	9 cut(s)	102, 123, 274, 739, 1664, 2066, 2113, 2533, 3520
HinP1I	GCGC	9 cut(s)	102, 123, 274, 739, 1664, 2066, 2113, 2533, 3520
HincII	GTYRAC	3 cut(s)	769, 2584, 3265
HindII	GTYRAC	3 cut(s)	769, 2584, 3265
HindIII	AAGCTT	5 cut(s)	875, 1850, 2130, 2300, 3738
HpaI	GTTAAC	1 cut(s)	2584
HphI	GGTGA	8 cut(s)	1685, 2359, 3446, 3622, 3760, 3802, 4024, 5140
Hpy99I	CGWCG	5 cut(s)	626, 773, 776, 2823, 4336
HpyCH4IV	ACGT	5 cut(s)	222, 438, 771, 3807, 4339
HpySE526I	ACGT	5 cut(s)	222, 438, 771, 3807, 4339
Hsp92I	GRCGYC	3 cut(s)	318, 771, 2533
HspAI	GCGC	9 cut(s)	102, 123, 274, 739, 1664, 2066, 2113, 2533, 3520
KasI	GGCGCC	1 cut(s)	2532
Kpn2I	TCCGGA	1 cut(s)	16
Ksp22I	TGATCA	2 cut(s)	2221, 2295
KspAI	GTTAAC	1 cut(s)	2584
LguI	GCTCTTC	1 cut(s)	2178
MabI	ACCWGGT	1 cut(s)	2340
MaeI	CTAG	5 cut(s)	2502, 3891, 4098, 4995, 5190
MaeII	ACGT	5 cut(s)	222, 438, 771, 3807, 4339
MaeIII	GTNAC	8 cut(s)	790, 1451, 2197, 2618, 3060, 3628, 3808, 5242
MfeI	CAATTG	1 cut(s)	4413
MflI	RGATCY	5 cut(s)	399, 1611, 2510, 3199, 4818
MlsI	TGGCCA	3 cut(s)	260, 524, 1262
MluNI	TGGCCA	3 cut(s)	260, 524, 1262
Mly113I	GGCGCC	1 cut(s)	2533
MlyI	GAGTC	3 cut(s)	2555, 3712, 5134
MmeI	TCCRAC	8 cut(s)	414, 438, 1714, 1884, 3109, 3486, 3832, 4938
Mox20I	TGGCCA	3 cut(s)	260, 524, 1262
Mph1103I	ATGCAT	3 cut(s)	3678, 4033, 5212
MroI	TCCGGA	1 cut(s)	16
MroXI	GAANNNNTTC	3 cut(s)	608, 2801, 3246
MscI	TGGCCA	3 cut(s)	260, 524, 1262
MslI	CAYNNNNRTG	8 cut(s)	524, 1604, 1671, 3210, 3690, 4007, 4036, 4487
Msp20I	TGGCCA	3 cut(s)	260, 524, 1262
MspA1I	CMGCKG	2 cut(s)	2267, 3643
MspCI	CTTAAG	1 cut(s)	2948
MunI	CAATTG	1 cut(s)	4413
Mva1269I	GAATGC	6 cut(s)	610, 2188, 2820, 3678, 5006, 5210
MvnI	CGCG	6 cut(s)	68, 102, 113, 123, 426, 2066
NarI	GGCGCC	1 cut(s)	2533
NciI	CCSGG	5 cut(s)	23, 571, 598, 650, 1208
NcoI	CCATGG	1 cut(s)	525
NdeI	CATATG	1 cut(s)	3154
NmeAIII	GCCGAG	1 cut(s)	679
NmuCI	GTSAC	4 cut(s)	2197, 3060, 3628, 3808
NsbI	TGCGCA	1 cut(s)	1665
NsiI	ATGCAT	3 cut(s)	3678, 4033, 5212
NspI	RCATGY	4 cut(s)	815, 1609, 3168, 3569
NspV	TTCGAA	3 cut(s)	1681, 3250, 3670
PaeI	GCATGC	1 cut(s)	3168
PaeR7I	CTCGAG	2 cut(s)	3720, 4190
PagI	TCATGA	1 cut(s)	3121
PceI	AGGCCT	2 cut(s)	953, 1386
PciSI	GCTCTTC	1 cut(s)	2178
PctI	GAATGC	6 cut(s)	610, 2188, 2820, 3678, 5006, 5210
PdmI	GAANNNNTTC	3 cut(s)	608, 2801, 3246
PflFI	GACNNNGTC	2 cut(s)	221, 1397
PflMI	CCANNNNNTGG	2 cut(s)	396, 5022
PfoI	TCCNGGA	2 cut(s)	180, 3071
PinAI	ACCGGT	1 cut(s)	4691
PleI	GAGTC	3 cut(s)	2555, 3712, 5134
PluTI	GGCGCC	1 cut(s)	2536
PpsI	GAGTC	3 cut(s)	2555, 3712, 5134
PpuMI	RGGWCCY	1 cut(s)	843
PsiI	TTATAA	2 cut(s)	1845, 2168
Psp124BI	GAGCTC	3 cut(s)	432, 1257, 2874
Psp5II	RGGWCCY	1 cut(s)	843
PspFI	CCCAGC	1 cut(s)	4647
PspPPI	RGGWCCY	1 cut(s)	843
PspXI	VCTCGAGB	1 cut(s)	3720
PsrI	GAACNNNNNNTAC	6 cut(s)	1397, 1429, 3095, 3127, 4060, 4092
PstI	CTGCAG	3 cut(s)	1026, 1120, 2464
PstNI	CAGNNNCTG	1 cut(s)	2115
PsuI	RGATCY	5 cut(s)	399, 1611, 2510, 3199, 4818
PsyI	GACNNNGTC	2 cut(s)	221, 1397
RseI	CAYNNNNRTG	8 cut(s)	524, 1604, 1671, 3210, 3690, 4007, 4036, 4487
SacI	GAGCTC	3 cut(s)	432, 1257, 2874
SalI	GTCGAC	1 cut(s)	767
SapI	GCTCTTC	1 cut(s)	2178
ScaI	AGTACT	2 cut(s)	725, 2410
SchI	GAGTC	3 cut(s)	2555, 3712, 5134
SexAI	ACCWGGT	1 cut(s)	2340
SfcI	CTRYAG	3 cut(s)	1022, 1116, 2460
SfoI	GGCGCC	1 cut(s)	2534
Sfr274I	CTCGAG	2 cut(s)	3720, 4190
SfuI	TTCGAA	3 cut(s)	1681, 3250, 3670
SinI	GGWCC	8 cut(s)	19, 70, 97, 175, 843, 1161, 3371, 4909
SlaI	CTCGAG	2 cut(s)	3720, 4190
SmiMI	CAYNNNNRTG	8 cut(s)	524, 1604, 1671, 3210, 3690, 4007, 4036, 4487
SmlI	CTYRAG	6 cut(s)	755, 1375, 2588, 2948, 3720, 4190
SmoI	CTYRAG	6 cut(s)	755, 1375, 2588, 2948, 3720, 4190
SpeI	ACTAGT	2 cut(s)	4097, 4994
SphI	GCATGC	1 cut(s)	3168
SseBI	AGGCCT	2 cut(s)	953, 1386
SspDI	GGCGCC	1 cut(s)	2532
SspI	AATATT	2 cut(s)	2575, 3299
SspMI	CTAG	5 cut(s)	2502, 3891, 4098, 4995, 5190
SstI	GAGCTC	3 cut(s)	432, 1257, 2874
StuI	AGGCCT	2 cut(s)	953, 1386
StyI	CCWWGG	5 cut(s)	525, 1283, 3051, 3633, 3882
TaiI	ACGT	5 cut(s)	225, 441, 774, 3810, 4342
TaqII	GACCGA	1 cut(s)	3717
TatI	WGTACW	7 cut(s)	723, 1404, 2106, 2408, 2712, 2763, 3111
TauI	GCSGC	9 cut(s)	107, 110, 113, 148, 166, 318, 426, 473, 911
TscAI	CASTG	9 cut(s)	697, 994, 1785, 2683, 2704, 3766, 4069, 4165, 5023
TseFI	GTSAC	4 cut(s)	2197, 3060, 3628, 3808
Tsp45I	GTSAC	4 cut(s)	2197, 3060, 3628, 3808
TspGWI	ACGGA	2 cut(s)	580, 2432
TspRI	CASTG	9 cut(s)	697, 994, 1785, 2683, 2704, 3766, 4069, 4165, 5023
Tth111I	GACNNNGTC	2 cut(s)	221, 1397
Van91I	CCANNNNNTGG	2 cut(s)	396, 5022
Vha464I	CTTAAG	1 cut(s)	2948
VpaK11BI	GGWCC	8 cut(s)	19, 70, 97, 175, 843, 1161, 3371, 4909
XapI	RAATTY	7 cut(s)	1972, 2800, 3216, 3512, 3666, 4183, 4961
XceI	RCATGY	4 cut(s)	815, 1609, 3168, 3569
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1072, 1974
XhoI	CTCGAG	2 cut(s)	3720, 4190
XmiI	GTMKAC	3 cut(s)	768, 2061, 4795
XmnI	GAANNNNTTC	3 cut(s)	608, 2801, 3246
XspI	CTAG	5 cut(s)	2502, 3891, 4098, 4995, 5190
ZraI	GACGTC	1 cut(s)	772
ZrmI	AGTACT	2 cut(s)	725, 2410
Zsp2I	ATGCAT	3 cut(s)	3678, 4033, 5212

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.