Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0014998

Physical Location & Seq

Genomic Coordinates Reverse (-)

28947722 .. 28962664

Gene Structure 14943 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1240300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5310 bp

ATGAGTATCCAGCGGGGTACTCAATCCTCCTCCTCCGACCCCGACTCGGAGCCCTACAATATCATCCCCGTCCACGACCTCCTCGCCGACCACCCCTCCCTCCGTTTCCCAGAGGTACGCGCCGCCGCCGCCTCCCTACGCGCCGTTGGCAACCTCCGCCGTCCCCCTTACTCCCAATGGCAGCCCCACATGGACCTCCTCGACTGGCTCGCCCTCTTCTTCGGCTTCCAGTACGACAACGTCCGCAACCAGCGCGAGCACATAGTTCTCCACCTCGCCAACGCTCAGATGCGCCTCAACCCTCCCCCGGACAACATCGACACACTCGACGGCGGCGTTTTGAGGAAGTTCCGGAAAAAGCTGCTCAAGAATTACACCAGCTGGTGCTCCTATTTGGGGAAGAAGTCCAATATTTGGATCTCCGACCGGAGGCGTGATACCGCCGCCGACCAGCGCCGGGAGCTGCTGTACGTGTCACTGTACCTGCTGATCTGGGGCGAGGCGGCGAATCTGAGGTTTGTCCCCGAGTGCTTGTGCTTTATTTTTCATAACATGGCCATGGAATTGAACAAGATCTTGGAGGATTATATTGATGAGGAGACGGGTCAGCCCGTGATGCCGTCGACGTCGGGGGAGAATGCGTTCTTGAATAGTGTTGTGAAGCCCATATACGAGACGATTCGAGCCGAGGTGGAGAGCAGCAAGAATGGGACTGCCCCTCATAGTGTGTGGAGGAACTATGATGATATCAATGAGTATTTCTGGAGTAAGAGGTGTTTTGACAAGCTCAGGTGGCCTGTCGATGTTGGGAGTAACTTTTTTGTGACTTCTAGTAGGAGTAGGCATGTGGGGAAGACTGGGTTTGTGGAGCAGAGGTCCTTTTGGAACTTGTTTCGGAGCTTCGACAAGCTCTGGATCATGTTGATTATGTTCCTCCAGGCCGCTATTATTGTTGCTTGGGAGGAGAGGGAGTTTCCATGGCAGGCGTTGGAGGAGAGGGAAGTGCAGGTCAAGGTTTTGACTGTGTTTTTCACTTGGGCTGGTTTGAGGTTTTTGCAGTCGTTGCTTGATGTGGGAATGCAGTATAGTCTGGTTTCGAGGGAGACTTTGGGGCTCGGAGTGAGGATGGTGTTGAAGAGCATAGTTGCTGCCCTGTGGATTGTTGTTTTTGGGGTGTTCTATGGGAGGATATGGACGCAGAGAAATCAGGATCGGAGGTGGTCCACCGAGGCAAATAATCGGGTGGTGAATTTTCTTACGGTGGCAGTAGTTTTCATCCTGCCAGAGCTTTTGGCTTTGGTCCTCTTTATTCTTCCTTGGGTTCGGAATTTTCTTGAGAACACAAACTGGAAGATATTTTACATGTTATCGTGGTGGTTTCAAAGCAGAACTTTTGTTGGCCGTGGCTTGAGAGAAGGCCTTGTGGACAATATAAAGTACAGTTTGTTCTGGATTTTCGTGCTTGCTACGAAATTTTCTTTCAGCTACTTCATGCAGATCAAACCCCTGATTCGCCCGTCAAAAGAACTCATAAAACTTAATAATGTGAACTATGAGTGGCATCAGTTTTTTGGCAACAGCAACAAATTTGCAGTGGGATTGCTGTGGCTTCCAGTCGTTTTGATATACCTCATGGATTTGCAGCTGTTTTATTCCATTTACTCCTCTCTTGTGGGGGCAGGAGTGGGCTTGTTTGCACATTTGGGCGAGATACGAAATATCGGGCAATTGAGGCTAAGATTCCAGTTCTTTGCAGGTGCAATTCAATTTAATCTCATGCCAGAAGAGCAACTGTTAAATGCAAGGGGAACTCTGAGGAGTAAGTTTAATGATGCCATCCACCGGTTGAAGCTGAGGTATGGGCTTGGACGGCCTTATAAGAAGCTCGAATCCAACCAGGTTGAGGCAACCAGGTTTGCTTTGATATGGAATGAGATCATTTTGATCTTCAGGGAAGAAGATATAATTTCTGACAGCGAGCTTGAGCTGTTAGAGCTGCCACAAAATTCTTGGAATATCAGGGTTATTCGGTGGCCTTGTTTTCTTCTTTGCAATGAACTGCTGCTGGCACTTAGTCAGGCCAAAGAGTTAGTAGATGCTCCTGATAAGTGGCTCTGGTATAAGATATGCAAGAATGAGTACAGGCGCTGCGCTGTTATAGAAGCTTATGGTTGCATCAAGCACTTGCTACTTGACATCATCAAACGCAACACAGAAGAACATTCCATTATGACAGTCTTGTTTCAGGAAATTGATCACTCTCTTGAGATTGAGAAGTTCACGAAAACATTTAAAACGACGGCGCTTCCAGAGCTGCATGCCAAGTTGATCAAACTTGTTGAGCTTTTGAAAAAACCCAAGCAAGACACAAACCAGGTGGTAAATACTCTGCAGGCCCTTTATGAGATTGCCATTCGAGATTTTTTCAAGGAAAAAAGAAGTACTGAGCAGCTGATGGAGGATGGTTTGGCCCCTCGTAATCCAGGTTCTGCTGCAGGGCTGCTTTTTGAAAATGCTGTTGAGTTGCCTGATCCTAACAATGTATTCTTCTATAGGCAGGTTAGGCGCTTGCACACAATACTTACCTCTCGGGACTCAATGCAGAATATCCCAGTTAACCTTGAGGCAAGACGCAGAATTGCCTTCTTCAGTAACTCCTTGTTTATGAACATACCCCATGCACCTCAAGTTGAGAAAATGATGGCCTTCAGTGTGCTGACCCCGTACTACAGTGAAGAAGTATTGTACAACAAAGAACAACTACGTACGGAGAATGAAGATGGGGTTTCTACCCTGTATTATTTACAGACAATTTATGTTGACGAGTGGAAAAATTTTATGGAGAGGATGCGCAGAGAAGGAATGGGGAGTGATGATGAAATATGGTCAACTAAGTTGAGAGAACTCAGGCTCTGGGCTTCTTACAGAGGCCAAACACTTACAAGGACCGTAAGGGGAATGATGTACTACTATCGGGCTCTGAAGATGTTGGCTTTTCTGGATTCTGCATCCGAGATGGACATTCGGGAAGGATCACAAGAACCTGGTTCCACGATGCAAGACATTGGCTTGGACAGATTGACTTCTGAGAGGTCACCTTCTTCCCTAAGTTTAACTAGAACAAGCAGTTGTGTGGACTCATTGTACAAAGGTCATGAATATGGAACTGCTTTGATGAAATTTACATATGTGGTTGCATGCCAGATATACGGAACACAAAAAGTGAAAAAAGATCCCCATGCTGATGAAATTTTGTATTTGATGCAAAACAACGAAGCACTTCGAGTTGCATATCTTGATGAGGTTTCAACTGGCAGGAATGAGAAGGAGTACTATTCTGTTCTTGTGAAGTATGATCAGAAATTAGAGAAGGAGGTGGAAATCTACCGCATAAAGTTGCCTGGTCCAATGAAGCTCGGAGAGGGAAAACCAGAGAATCAAAATCATGCCATTATCTTCACTCGTGGTGATGCAGTTCAGACGATTGATATGAACCAAGACAATTATTTTGAGGAGGCACTCAAAATGCGGAATCTATTGGAAGAGTACAGGCACTATTATGGCATCCGGAAGCCTACTATCTTGGGAGTCAGAGAGCATATTTTTACTGGTTCCGTCTCATCACTTGCCTGGTTTATGTCAGCTCAAGAAACAAGTTTTGTCACCTTGGGGCAACGGGTGTTGGCGAACCCCTTGAAAATTAGAATGCATTATGGTCATCCAGATGTGTTTGACAGATTTTGGTTCTTGACTCGAGGTGGCATCAGTAAAGCTTCCAGGGTCATTAACATCAGTGAAGACATTTTTGCTGGCTTTAATTGTACGTTGCGGGGTGGAAATGTCACACACCATGAATACATTCAAGTCGGCAAGGGAAGGGATGTGGGATTCAATCAAATTTCCATGTTTGAGGCCAAGGTTGCTAGTGGGAACGGGGAACAAATTCTTAGCAGAGATGTCTATAGATTGGGTCACAGGCTGGATTTCTTGCGGATGCTATCATTCTTTTACACTACAGTGGGATTCTTTTTTAATACAATGGTTGTTGTTCTTACTGTGTATACATTTCTATGGGGCCGGCTTTATCTGGCTCTTAGTGGTCTTGAAGGTTCTATATTGGGAAATGATTCTACTAACAGAGCACTTGCTACAGTCTTGAATCAGCAGTTCATTATCCAACTTGGTGTGTTCACTGCCCTTCCAATGATAGTGGAGAATTCCCTTGAACATGGATTCCTTCATGCTATTTGGGATTTCTTGACGATGCAGCTCCAGCTTTCATCAGTTTTCTACACATTCTCAATGGGAACTCGTACCCACTTTTTTGGGCGAACTATTCTTCATGGTGGTGCAAAGTATCGTGCAACTGGACGTGGCTTTGTTGTGCAGCACAAAAGTTTTGCAGAGAATTATAGGCTCTATGCTCGTAGTCATTTTGTGAAAGCAATTGAACTAGGGTTGATACTTATTGTTTATGCATCGCATAGTCCTGTAGCTAAGGACACTTTTATTTACATAGGCATGACCATCACGAGTTGGTTCCTGGTCGTGTCATGGTTTATGGCCCCCTTCGTTTTCAATCCTTCTGGCTTTGATTGGTTGAAGACTGTAGATGACTTTGATGACTTCATGACCTGGATTTGGTATCGTGGCAGTGTCTTCGCAAAAGCTGAACAGAGCTGGGAAAGATGGTGGTATGAGGAGCAAGATCATCTAAGGACCACTGGCCTCTGGGGAAAGTTTCTAGAGATAATTTTAGACCTGCGCTTCTTCTTTTTCCAATATGGGATTGTATACCAGCTTGGTATTGCTGCTGGAAGTACCAGTATTGCTGTTTACTTGTTGTCTTGGATCTTTGTGTTTGTGGCTTTTGGCATTTTTGTGGTGATAGCATATGCTCGGGACAGATATGCCTCAAAAGATCACATTTACTATCGGCTGGTGCAATTTCTTGTCATAAAACTTGGATTAATTGTTATGATTGCCCTGCTGGAATTCACCGAGTTCAAGTTCATGGATATTTTCACTAGTCTTCTGGCATTCGTTCCGACTGGGTGGGGATTGGTGTTGATAGCACAAGTATTTCGGCCCTGTCTACAGCGCACTATACTGTGGGCGGCGGTTGTTTCTGTGGCTCGACTGTATGATATATTGTTTGGGGTGATTGTAATGACACCTGTGGCAGTATTATCATGGTTTCCTGGTTTTCAGTCTATGCAGACTAGGATATTGTTTAATGAAGCATTCAGCCGGGGCCTTCGTATATTCCAGATTGTTACGGGAAAAAAGTCCAAGTCAGATAAGTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0005911 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0009506 GO:0009555 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009653 GO:0009719 GO:0009725 GO:0009751 GO:0009863 GO:0009870 GO:0009965 GO:0009987 GO:0010016 GO:0010033 GO:0010150 GO:0012505 GO:0014070 GO:0016020 GO:0016043 GO:0023052 GO:0030054 GO:0031347 GO:0031349 GO:0032501 GO:0032502 GO:0032870 GO:0033036 GO:0033037 GO:0033554 GO:0035690 GO:0042221 GO:0042493 GO:0042545 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045089 GO:0045229 GO:0046677 GO:0048229 GO:0048366 GO:0048367 GO:0048518 GO:0048583 GO:0048584 GO:0048731 GO:0048827 GO:0048856 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051179 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0055044 GO:0065007 GO:0070727 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071407 GO:0071446 GO:0071495 GO:0071554 GO:0071555 GO:0071840 GO:0071944 GO:0080134 GO:0090693 GO:0098542 GO:0099402 GO:1901700 GO:1901701 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FKS1_dom1 Glucan_synthase

Protein Analysis

1770

Amino Acids

205.94

Weight (kDa)

8.9

Isoelectric Point (pI)

39.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FKS1_dom1	PF14288	154 - 266	2.8e-45	1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1	PF25968	679 - 862	5.5e-31	Callose synthase 1 domain
Glucan_synthase	PF02364	870 - 1664	3.2e-262	1,3-beta-glucan synthase component

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014998)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G03550
fragaria_vesca	FvH4_6g26200
malus_domestica	MD09G1247400.v1.1 MD17G1240300.v1.1
prunus_persica	Prupe.3G138400_v2.0.a1
pyrus_communis	pycom17g24360
rosa_chinensis	RchiOBHm_Chr2g0126481
rosa_laevigata	RLG00000018906
rosa_multiflora	Rmu_sc0000662.1_g000007
rosa_roxburghii	Rroxscaffold_2G00117140
rosa_rugosa	Rorug02G0263500
rosa_samantha	Rh2AG320100 Rh2BG328800 Rh2CG308500 Rh2DG348100
rosa_wichuraiana	Rw2G025950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1878
AarI	CACCTGC	1 cut(s)	1745
AatII	GACGTC	1 cut(s)	629
Acc16I	TGCGCA	1 cut(s)	2852
Acc36I	ACCTGC	5 cut(s)	492, 997, 1745, 2548, 4770
AccB7I	CCANNNNNTGG	1 cut(s)	414
AccI	GTMKAC	4 cut(s)	623, 4061, 4794, 5095
AccII	CGCG	3 cut(s)	120, 141, 255
AccIII	TCCGGA	2 cut(s)	351, 3567
AclWI	GGATC	7 cut(s)	425, 923, 1218, 2525, 3040, 3227, 4859
AcoI	YGGCCR	2 cut(s)	555, 1399
AcuI	CTGAAG	4 cut(s)	1933, 2632, 2692, 3002
AcyI	GRCGYC	1 cut(s)	626
AfiI	CCNNNNNNNGG	9 cut(s)	46, 307, 396, 414, 429, 457, 1842, 1903, 4641
AflIII	ACRYGT	2 cut(s)	471, 1362
AgeI	ACCGGT	1 cut(s)	1842
AhlI	ACTAGT	1 cut(s)	5027
AjiI	CACGTC	1 cut(s)	4373
AjuI	GAANNNNNNNTTGG	2 cut(s)	560, 592
AleI	CACNNNNGTG	1 cut(s)	4016
AloI	GAACNNNNNNTCC	4 cut(s)	626, 658, 4218, 4250
Alw21I	GWGCWC	3 cut(s)	261, 389, 4144
Alw26I	GTCTC	4 cut(s)	593, 668, 1097, 3622
AlwI	GGATC	7 cut(s)	425, 923, 1218, 2525, 3040, 3227, 4859
AlwNI	CAGNNNCTG	3 cut(s)	2148, 2489, 2913
Ama87I	CYCGRG	4 cut(s)	524, 2589, 3753, 4899
Aor13HI	TCCGGA	2 cut(s)	351, 3567
ArsI	GACNNNNNNTTYG	2 cut(s)	3491, 3523
AseI	ATTAAT	1 cut(s)	4970
AsiGI	ACCGGT	1 cut(s)	1842
Asp700I	GAANNNNTTC	5 cut(s)	641, 3279, 3858, 3942, 4126
AsuC2I	CCSGG	3 cut(s)	308, 458, 5252
AsuHPI	GGTGA	7 cut(s)	1258, 3087, 3479, 3655, 4897, 4990, 5173
AvaI	CYCGRG	4 cut(s)	524, 2589, 3753, 4899
AvaII	GGWCC	7 cut(s)	193, 876, 1221, 1300, 2946, 3404, 4719
BalI	TGGCCA	1 cut(s)	557
BanII	GRGCYC	3 cut(s)	54, 1116, 2980
BarI	GAAGNNNNNNTAC	4 cut(s)	653, 685, 1343, 1375
BauI	CACGAG	2 cut(s)	3462, 4531
BbsI	GAAGAC	5 cut(s)	860, 3804, 4610, 4651, 5024
Bbv12I	GWGCWC	3 cut(s)	261, 389, 4144
BbvCI	CCTCAGC	1 cut(s)	1853
BccI	CCATC	9 cut(s)	1120, 1844, 2449, 2456, 2695, 2774, 3010, 4535, 4683
BceAI	ACGGC	7 cut(s)	128, 144, 346, 604, 1386, 1886, 2316
BcgI	CGANNNNNNTGC	2 cut(s)	4642, 4676
BciVI	GTATCC	1 cut(s)	17
BclI	TGATCA	3 cut(s)	2254, 2328, 3355
BcnI	CCSGG	3 cut(s)	308, 458, 5252
BcoDI	GTCTC	4 cut(s)	593, 668, 1097, 3622
BcuI	ACTAGT	1 cut(s)	5027
BfaI	CTAG	7 cut(s)	831, 3117, 3924, 4454, 4745, 5028, 5223
BfoI	RGCGCY	4 cut(s)	457, 2149, 2306, 2569
BfuAI	ACCTGC	5 cut(s)	492, 997, 1745, 2548, 4770
BfuI	GTATCC	1 cut(s)	17
BglII	AGATCT	1 cut(s)	573
BmcAI	AGTACT	2 cut(s)	2443, 3332
Bme18I	GGWCC	7 cut(s)	193, 876, 1221, 1300, 2946, 3404, 4719
BmeT110I	CYCGRG	4 cut(s)	524, 2589, 3753, 4899
BmgBI	CACGTC	1 cut(s)	4373
BmiI	GGNNCC	8 cut(s)	51, 2472, 3049, 3613, 4076, 4541, 4566, 5255
BmrI	ACTGGG	3 cut(s)	867, 2606, 5061
BmuI	ACTGGG	3 cut(s)	867, 2606, 5061
BpiI	GAAGAC	5 cut(s)	860, 3804, 4610, 4651, 5024
BpmI	CTGGAG	3 cut(s)	784, 920, 4256
Bpu10I	CCTNAGC	3 cut(s)	788, 1853, 4497
BpuEI	CTTGAG	8 cut(s)	350, 1355, 1429, 2003, 2285, 2642, 2670, 3630
BpuMI	CCSGG	3 cut(s)	308, 458, 5252
BsaAI	YACGTR	2 cut(s)	472, 2765
BsaHI	GRCGYC	1 cut(s)	626
BsaWI	WCCGGW	4 cut(s)	351, 426, 1842, 3567
Bsc4I	CCNNNNNNNGG	9 cut(s)	46, 307, 396, 414, 429, 457, 1842, 1903, 4641
Bse118I	RCCGGY	2 cut(s)	1842, 4077
Bse3DI	GCAATG	1 cut(s)	2059
BseAI	TCCGGA	2 cut(s)	351, 3567
BseLI	CCNNNNNNNGG	9 cut(s)	46, 307, 396, 414, 429, 457, 1842, 1903, 4641
BseMI	GCAATG	1 cut(s)	2059
BseMII	CTCAG	8 cut(s)	299, 503, 802, 1805, 1844, 2436, 2920, 3078
BseYI	CCCAGC	1 cut(s)	4680
BsgI	GTGCAG	2 cut(s)	1025, 4406
Bsh1236I	CGCG	3 cut(s)	120, 141, 255
Bsh1285I	CGRYCG	1 cut(s)	427
BshTI	ACCGGT	1 cut(s)	1842
BsiEI	CGRYCG	1 cut(s)	427
BsiHKAI	GWGCWC	3 cut(s)	261, 389, 4144
BsiHKCI	CYCGRG	4 cut(s)	524, 2589, 3753, 4899
BsiSI	CCGG	8 cut(s)	308, 352, 427, 457, 1843, 3568, 4078, 5251
BsiWI	CGTACG	1 cut(s)	2765
BslFI	GGGAC	5 cut(s)	147, 506, 724, 2606, 4916
BslI	CCNNNNNNNGG	9 cut(s)	46, 307, 396, 414, 429, 457, 1842, 1903, 4641
BsmAI	GTCTC	4 cut(s)	593, 668, 1097, 3622
BsmBI	CGTCTC	3 cut(s)	593, 668, 3622
BsmFI	GGGAC	5 cut(s)	147, 506, 724, 2606, 4916
BsmI	GAATGC	5 cut(s)	643, 1083, 3711, 5039, 5243
BsoBI	CYCGRG	4 cut(s)	524, 2589, 3753, 4899
Bsp1286I	GDGCHC	6 cut(s)	54, 261, 389, 1116, 2980, 4144
Bsp13I	TCCGGA	2 cut(s)	351, 3567
Bsp1407I	TGTACA	2 cut(s)	2745, 3144
Bsp19I	CCATGG	2 cut(s)	558, 977
BspCNI	CTCAG	8 cut(s)	298, 504, 801, 1806, 1845, 2437, 2919, 3079
BspEI	TCCGGA	2 cut(s)	351, 3567
BspFNI	CGCG	3 cut(s)	120, 141, 255
BspHI	TCATGA	2 cut(s)	3154, 4629
BspLI	GGNNCC	8 cut(s)	51, 2472, 3049, 3613, 4076, 4541, 4566, 5255
BspMAI	CTGCAG	2 cut(s)	2394, 2497
BspMI	ACCTGC	5 cut(s)	492, 997, 1745, 2548, 4770
BspPI	GGATC	7 cut(s)	425, 923, 1218, 2525, 3040, 3227, 4859
BspQI	GCTCTTC	2 cut(s)	1130, 1779
BsrDI	GCAATG	1 cut(s)	2059
BsrFI	RCCGGY	2 cut(s)	1842, 4077
BsrGI	TGTACA	2 cut(s)	2745, 3144
BssAI	RCCGGY	2 cut(s)	1842, 4077
BssNAI	GTATAC	2 cut(s)	4062, 4795
BssNI	GRCGYC	1 cut(s)	626
BssSI	CACGAG	2 cut(s)	3462, 4531
BssT1I	CCWWGG	5 cut(s)	558, 977, 1316, 3666, 3915
Bst1107I	GTATAC	2 cut(s)	4062, 4795
Bst2BI	CACGAG	2 cut(s)	3462, 4531
Bst6I	CTCTTC	4 cut(s)	221, 1130, 1779, 3537
BstACI	GRCGYC	1 cut(s)	626
BstAPI	GCANNNNNTGC	1 cut(s)	1063
BstAUI	TGTACA	2 cut(s)	2745, 3144
BstBAI	YACGTR	2 cut(s)	472, 2765
BstDSI	CCRYGG	3 cut(s)	558, 977, 1402
BstEII	GGTNACC	1 cut(s)	3093
BstFNI	CGCG	3 cut(s)	120, 141, 255
BstH2I	RGCGCY	4 cut(s)	457, 2149, 2306, 2569
BstMAI	GTCTC	4 cut(s)	593, 668, 1097, 3622
BstMCI	CGRYCG	1 cut(s)	427
BstNSI	RCATGY	4 cut(s)	848, 1366, 2321, 3201
BstPI	GGTNACC	1 cut(s)	3093
BstSNI	TACGTA	1 cut(s)	2765
BstUI	CGCG	3 cut(s)	120, 141, 255
BstV2I	GAAGAC	5 cut(s)	860, 3804, 4610, 4651, 5024
BstX2I	RGATCY	4 cut(s)	417, 573, 3232, 4851
BstXI	CCANNNNNNTGG	1 cut(s)	4325
BstYI	RGATCY	4 cut(s)	417, 573, 3232, 4851
BstZ17I	GTATAC	2 cut(s)	4062, 4795
BsuI	GTATCC	1 cut(s)	17
BtgI	CCRYGG	3 cut(s)	558, 977, 1402
BtgZI	GCGATG	1 cut(s)	4464
BtrI	CACGTC	1 cut(s)	4373
BtsI	GCAGTG	3 cut(s)	1599, 4191, 4660
BtsIMutI	CAGTG	9 cut(s)	476, 1599, 2716, 2737, 3799, 4023, 4191, 4660, 4722
BveI	ACCTGC	5 cut(s)	492, 997, 1745, 2548, 4770
CaiI	CAGNNNCTG	3 cut(s)	2148, 2489, 2913
CciI	TCATGA	2 cut(s)	3154, 4629
Cfr10I	RCCGGY	2 cut(s)	1842, 4077
CseI	GACGC	2 cut(s)	1204, 2640
CsiI	ACCWGGT	4 cut(s)	1896, 1910, 2373, 3043
CspAI	ACCGGT	1 cut(s)	1842
CspCI	CAANNNNNGTGG	4 cut(s)	2358, 2393, 4191, 4226
DraI	TTTAAA	1 cut(s)	2293
EaeI	YGGCCR	2 cut(s)	555, 1399
Eam1104I	CTCTTC	4 cut(s)	221, 1130, 1779, 3537
EarI	CTCTTC	4 cut(s)	221, 1130, 1779, 3537
EciI	GGCGGA	1 cut(s)	146
Eco105I	TACGTA	1 cut(s)	2765
Eco130I	CCWWGG	5 cut(s)	558, 977, 1316, 3666, 3915
Eco147I	AGGCCT	1 cut(s)	1419
Eco24I	GRGCYC	3 cut(s)	54, 1116, 2980
Eco32I	GATATC	1 cut(s)	748
Eco47I	GGWCC	7 cut(s)	193, 876, 1221, 1300, 2946, 3404, 4719
Eco57I	CTGAAG	4 cut(s)	1933, 2632, 2692, 3002
Eco88I	CYCGRG	4 cut(s)	524, 2589, 3753, 4899
Eco91I	GGTNACC	1 cut(s)	3093
EcoO109I	RGGNCCY	3 cut(s)	876, 2395, 5254
EcoO65I	GGTNACC	1 cut(s)	3093
EcoRI	GAATTC	2 cut(s)	4216, 4994
EcoRV	GATATC	1 cut(s)	748
EcoT14I	CCWWGG	5 cut(s)	558, 977, 1316, 3666, 3915
EcoT22I	ATGCAT	2 cut(s)	3711, 4480
EcoT38I	GRGCYC	3 cut(s)	54, 1116, 2980
ErhI	CCWWGG	5 cut(s)	558, 977, 1316, 3666, 3915
Esp3I	CGTCTC	3 cut(s)	593, 668, 3622
FalI	AAGNNNNNCTT	2 cut(s)	1375, 1407
FaqI	GGGAC	5 cut(s)	147, 506, 724, 2606, 4916
FauI	CCCGC	2 cut(s)	6, 3822
FauNDI	CATATG	2 cut(s)	3187, 4894
FbaI	TGATCA	3 cut(s)	2254, 2328, 3355
FblI	GTMKAC	4 cut(s)	623, 4061, 4794, 5095
FriOI	GRGCYC	3 cut(s)	54, 1116, 2980
FspBI	CTAG	7 cut(s)	831, 3117, 3924, 4454, 4745, 5028, 5223
FspI	TGCGCA	1 cut(s)	2852
GsaI	CCCAGC	1 cut(s)	4684
GsuI	CTGGAG	3 cut(s)	784, 920, 4256
HaeII	RGCGCY	4 cut(s)	457, 2149, 2306, 2569
HapII	CCGG	8 cut(s)	308, 352, 427, 457, 1843, 3568, 4078, 5251
HgaI	GACGC	2 cut(s)	1204, 2640
Hin1I	GRCGYC	1 cut(s)	626
HincII	GTYRAC	4 cut(s)	624, 2617, 2821, 2889
HindII	GTYRAC	4 cut(s)	624, 2617, 2821, 2889
HindIII	AAGCTT	2 cut(s)	2163, 3771
HpaI	GTTAAC	1 cut(s)	2617
HpaII	CCGG	8 cut(s)	308, 352, 427, 457, 1843, 3568, 4078, 5251
HphI	GGTGA	7 cut(s)	1258, 3087, 3479, 3655, 4897, 4990, 5173
Hpy99I	CGWCG	5 cut(s)	332, 625, 628, 631, 2302
HpyCH4IV	ACGT	6 cut(s)	240, 471, 626, 2764, 3824, 4372
HpySE526I	ACGT	6 cut(s)	240, 471, 626, 2764, 3824, 4372
Hsp92I	GRCGYC	1 cut(s)	626
Kpn2I	TCCGGA	2 cut(s)	351, 3567
KroI	GCCGGC	1 cut(s)	4077
KroNI	GCCGGC	1 cut(s)	4079
Ksp22I	TGATCA	3 cut(s)	2254, 2328, 3355
KspAI	GTTAAC	1 cut(s)	2617
LguI	GCTCTTC	2 cut(s)	1130, 1779
LmnI	GCTCC	8 cut(s)	49, 392, 460, 868, 897, 2104, 4275, 4702
MabI	ACCWGGT	4 cut(s)	1896, 1910, 2373, 3043
MaeI	CTAG	7 cut(s)	831, 3117, 3924, 4454, 4745, 5028, 5223
MaeII	ACGT	6 cut(s)	240, 471, 626, 2764, 3824, 4372
MaeIII	GTNAC	9 cut(s)	474, 812, 823, 2651, 3093, 3661, 3841, 3971, 5275
MfeI	CAATTG	2 cut(s)	1727, 4446
MflI	RGATCY	4 cut(s)	417, 573, 3232, 4851
MhlI	GDGCHC	6 cut(s)	54, 261, 389, 1116, 2980, 4144
MlsI	TGGCCA	1 cut(s)	557
MluNI	TGGCCA	1 cut(s)	557
MlyI	GAGTC	5 cut(s)	38, 2588, 3131, 3597, 3745
MmeI	TCCRAC	6 cut(s)	60, 447, 969, 1917, 4201, 5072
Mox20I	TGGCCA	1 cut(s)	557
Mph1103I	ATGCAT	2 cut(s)	3711, 4480
MroI	TCCGGA	2 cut(s)	351, 3567
MroNI	GCCGGC	1 cut(s)	4077
MroXI	GAANNNNTTC	5 cut(s)	641, 3279, 3858, 3942, 4126
MscI	TGGCCA	1 cut(s)	557
Msp20I	TGGCCA	1 cut(s)	557
MspA1I	CMGCKG	4 cut(s)	13, 381, 1645, 2452
MspI	CCGG	8 cut(s)	308, 352, 427, 457, 1843, 3568, 4078, 5251
MteI	GCGCNGCGC	1 cut(s)	2149
MunI	CAATTG	2 cut(s)	1727, 4446
Mva1269I	GAATGC	5 cut(s)	643, 1083, 3711, 5039, 5243
MvnI	CGCG	3 cut(s)	120, 141, 255
NaeI	GCCGGC	1 cut(s)	4079
NciI	CCSGG	3 cut(s)	308, 458, 5252
NcoI	CCATGG	2 cut(s)	558, 977
NdeI	CATATG	2 cut(s)	3187, 4894
NgoMIV	GCCGGC	1 cut(s)	4077
NlaIV	GGNNCC	8 cut(s)	51, 2472, 3049, 3613, 4076, 4541, 4566, 5255
NmeAIII	GCCGAG	1 cut(s)	712
NmuCI	GTSAC	6 cut(s)	474, 823, 3093, 3661, 3841, 3971
NsbI	TGCGCA	1 cut(s)	2852
NsiI	ATGCAT	2 cut(s)	3711, 4480
NspI	RCATGY	4 cut(s)	848, 1366, 2321, 3201
OliI	CACNNNNGTG	1 cut(s)	4016
PaeI	GCATGC	2 cut(s)	2321, 3201
PaeR7I	CTCGAG	1 cut(s)	3753
PagI	TCATGA	2 cut(s)	3154, 4629
PaqCI	CACCTGC	1 cut(s)	1745
PceI	AGGCCT	1 cut(s)	1419
PciI	ACATGT	1 cut(s)	1362
PciSI	GCTCTTC	2 cut(s)	1130, 1779
PcsI	WCGNNNNNNNCGW	2 cut(s)	324, 333
PctI	GAATGC	5 cut(s)	643, 1083, 3711, 5039, 5243
PdiI	GCCGGC	1 cut(s)	4079
PdmI	GAANNNNTTC	5 cut(s)	641, 3279, 3858, 3942, 4126
Pfl23II	CGTACG	1 cut(s)	2765
PflFI	GACNNNGTC	1 cut(s)	239
PflMI	CCANNNNNTGG	1 cut(s)	414
PinAI	ACCGGT	1 cut(s)	1842
PleI	GAGTC	5 cut(s)	38, 2588, 3131, 3596, 3745
PpsI	GAGTC	5 cut(s)	38, 2588, 3131, 3596, 3745
Ppu21I	YACGTR	2 cut(s)	472, 2765
PpuMI	RGGWCCY	1 cut(s)	876
PscI	ACATGT	1 cut(s)	1362
PshBI	ATTAAT	1 cut(s)	4970
PsiI	TTATAA	1 cut(s)	1878
Psp5II	RGGWCCY	1 cut(s)	876
PspEI	GGTNACC	1 cut(s)	3093
PspFI	CCCAGC	1 cut(s)	4680
PspLI	CGTACG	1 cut(s)	2765
PspN4I	GGNNCC	8 cut(s)	51, 2472, 3049, 3613, 4076, 4541, 4566, 5255
PspPPI	RGGWCCY	1 cut(s)	876
PspXI	VCTCGAGB	1 cut(s)	3753
PsrI	GAACNNNNNNTAC	4 cut(s)	1430, 1462, 2470, 2502
PstI	CTGCAG	2 cut(s)	2394, 2497
PstNI	CAGNNNCTG	3 cut(s)	2148, 2489, 2913
PsuI	RGATCY	4 cut(s)	417, 573, 3232, 4851
PsyI	GACNNNGTC	1 cut(s)	239
PvuII	CAGCTG	3 cut(s)	381, 1645, 2452
SalI	GTCGAC	1 cut(s)	622
SapI	GCTCTTC	2 cut(s)	1130, 1779
ScaI	AGTACT	2 cut(s)	2443, 3332
SchI	GAGTC	5 cut(s)	38, 2588, 3131, 3597, 3745
SduI	GDGCHC	6 cut(s)	54, 261, 389, 1116, 2980, 4144
SexAI	ACCWGGT	4 cut(s)	1896, 1910, 2373, 3043
Sfr274I	CTCGAG	1 cut(s)	3753
SgrDI	CGTCGACG	1 cut(s)	622
SinI	GGWCC	7 cut(s)	193, 876, 1221, 1300, 2946, 3404, 4719
SlaI	CTCGAG	1 cut(s)	3753
SmlI	CTYRAG	9 cut(s)	365, 1334, 1408, 1982, 2264, 2621, 2685, 3645, 3753
SmoI	CTYRAG	9 cut(s)	365, 1334, 1408, 1982, 2264, 2621, 2685, 3645, 3753
SnaBI	TACGTA	1 cut(s)	2765
SpeI	ACTAGT	1 cut(s)	5027
SphI	GCATGC	2 cut(s)	2321, 3201
SseBI	AGGCCT	1 cut(s)	1419
SspI	AATATT	1 cut(s)	412
SspMI	CTAG	7 cut(s)	831, 3117, 3924, 4454, 4745, 5028, 5223
StuI	AGGCCT	1 cut(s)	1419
StyI	CCWWGG	5 cut(s)	558, 977, 1316, 3666, 3915
TaiI	ACGT	6 cut(s)	243, 474, 629, 2767, 3827, 4375
TatI	WGTACW	8 cut(s)	1437, 2139, 2441, 2745, 2964, 3144, 3330, 3546
TauI	GCSGC	8 cut(s)	125, 128, 131, 336, 446, 506, 944, 5120
TscAI	CASTG	9 cut(s)	483, 1599, 2716, 2737, 3799, 4023, 4198, 4660, 4729
TseFI	GTSAC	6 cut(s)	474, 823, 3093, 3661, 3841, 3971
Tsp45I	GTSAC	6 cut(s)	474, 823, 3093, 3661, 3841, 3971
TspGWI	ACGGA	4 cut(s)	92, 2783, 3225, 3604
TspRI	CASTG	9 cut(s)	483, 1599, 2716, 2737, 3799, 4023, 4198, 4660, 4729
Tth111I	GACNNNGTC	1 cut(s)	239
Van91I	CCANNNNNTGG	1 cut(s)	414
VpaK11BI	GGWCC	7 cut(s)	193, 876, 1221, 1300, 2946, 3404, 4719
VspI	ATTAAT	1 cut(s)	4970
XbaI	TCTAGA	1 cut(s)	4744
XceI	RCATGY	4 cut(s)	848, 1366, 2321, 3201
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2007
XhoI	CTCGAG	1 cut(s)	3753
XmiI	GTMKAC	4 cut(s)	623, 4061, 4794, 5095
XmnI	GAANNNNTTC	5 cut(s)	641, 3279, 3858, 3942, 4126
XspI	CTAG	7 cut(s)	831, 3117, 3924, 4454, 4745, 5028, 5223
ZraI	GACGTC	1 cut(s)	627
ZrmI	AGTACT	2 cut(s)	2443, 3332
Zsp2I	ATGCAT	2 cut(s)	3711, 4480

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.