Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0014998

Physical Location & Seq

Genomic Coordinates Reverse (-)

44644448 .. 44649763

Gene Structure 5316 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00117140.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5316 bp

ATGAGTATCCGGCAGGGTACTCCTCCGGCCCAAGCCCCCGACCCGGAATCCGAGCCCTACAACATCATCCCCGTCCACAACCTCCTCGCCGACCACCCCTCTCTCCGCTTCCCCGAGGTCCGCGCCGCCGCCGCCGCCCTCCGCGCCGTCGGCAACCTCCGCAGGCCCCCCTACGCCCAATGGCGCGACCACATGGACCTCCTCGACTGGCTCGCCTTGTTCTTCGGCTTCCAATACGACAACGTCCGGAACCAGCGGGAGCACATCGTCCTCCACCTCGCCAACGCCCAGATGCGCCTGAATCCTCCGCCGGACAACATCGACACTCTCGACGGCGCCGTTTTGCGCAAGTTCCGCAAGAAGCTCCTCCTCAATTACACCAACTGGTGCTCCTATCTGGGGAAGAAGTCCAACATTTGGATCTCCGACCACCACCGCCGCGACGCCGCCGCCGACCACCGCCGCGAGCTCCTCTACGTCGGCCTCTATCTCCTGATCTGGGGCGAGGCCGCCAATCTCAGGTTCGTGCCCGAGTGCTTGTGCTATATATTTCATAACATGGCCATGGAGTTGAATAAGATTCTGGAGGATTATATTGATGAGAGTACGGGGCAGGCGGTGATGCCCTCCGTTTCGGGGGAGAATGCCTTCTTGAATTGTGTTGTCAAGCCCATTTATGAGACCATTAGGGCGGAGGTTGAGGGCAGCAAGAATGGGACTGCCCCACACAGTGTTTGGAGGAACTATGATGATATCAATGAGTACTTTTGGAGCAAGCGGTGTTTCGAAAAGCTCAAGTGGCCTCTAGATATTGGCAGCAACTTCTTTGTGACTAACAGTAAGAGTAAGCATGTGGGGAAGACTGGTTTTGTGGAGCAGAGGTCCTTCTGGAACTTGTTTAGGAGCTTTGACAAGCTCTGGATCATGTTGTTCTTGTTCCTTCAGGCCGCGATTATTGTTGCTTGGGAGGAGAAGGAGTATCCCTGGCAGGCCTTGGAGGATAGGGACGTCCAGGTCAGGGTGCTCACTGTGTTTTTCACGTGGACTGGTTTGAGGTTTCTGCAGTCCATACTTGATGTCGGGATGCAGTATAATCTGGTTTCCAGGGAGACTATGGGGCTCGGAGTCAGGATGGTCTGCAAGAGCATTGCTGCTGCATCCTGGATTATTGTTTTCGGGGTCTTTTATGGGAGGATATGGTCCCAGAGAAACCTTGATAGGCGGTGGTCTGCTGAGGCCGACAGGCGGGTTATGCAGTTTCTTTGGGTGGCATTGGTTTTCATCATTCCTGAGCTTTTGGCTGTGGCCTTCTTTATTCTTCCTTGGATTCGCAATTTTATGGAGAACTCAAACTGGCGGATATTTTATGCCTTATCCTGGTGGTTTCAGAGCAGAACTTTTGTTGGCCGTGGCTTGAGGGAAGGCCTTGTGGACAATGTCAAGTACACTCTGTTCTGGATTTTGGTGCTTTCTACCAAGTTTGCTTTCAGTTACTTCATGCTGATTAAACCCATGATTGTCCCATCTAAAGCACTTGTAAAACTTGATAATGTGGAGTATGAGTGGTATCAGCCTTTCAAAAACAGCAACAAAATGTCAGTGGGATTGTTGTGGCTTCCTGTTGTTTTGATATACCTGATGGACATGCAGATCTGGTATTCGATCTACTCCTCATTTTGGGGGGCATTAGTTGGGTTGCTTGCGCATTTGGGTGAGATTCGAAATATCCAACAATTGAGGCTGAGATTCCAGTTCTTTGCAAGTGCAATTCAGTTTAATCTCATGCCAGAAGAGCAGATGTTAAATGCAAGGGGGACACTGAAAAGTAAGTTTAATGATGCTATCCACAGGTTGAAGCTGAGGTATGGGCTTGGACAGCCTTATAAGAAGCTTGAGTCCAACCAGATCGAGGCAACCAAGTTTGCGTTGATATGGAATGAGATAATATTGATCTTCAGGGAAGAAGATTTAATTTCTGACTGCGAGGTTGAGTTGTTAGAGCTACCACAGAATTCTTGGAATGTCAGGGTCATTCGCTGGCCTTGTTTCCTTCTGTGCAATGAACTGTTGCTTGCACTGAGTCAGGCCAAAGAGTTGATAGATGCTCCTGACAAGTGGCTCTGGTATAAGATTTGCAAGAATGAGTACAGGCGCTGTGCTGTGATAGAAGCTTATGATTGTATCAAGCACTTGATACTTGCCATTATAAAAACCAACACAGAAGAGCATTCAATTGTGACAGTCTTGTTTCAGGAAATTGATCACTCCATTCAGATTGAGAAGTTCACAAAAACTTTTAAAACCACTGCCCTTCCACAACTCCATGCCAAGTTGATCAAACTTTCTGAGCTGTTGAACAAACCTAAGAAAGATGCAAACCAGGTGGTGAATACCCTTCAGGCTCTTTATGAGATTGCCATTCGAGATTTCTTCAAGGAGAAAAGAAAAAATGAACAGCTCATTGAGGATGGTTTGGCTCTCCGTGATCCGTCTTCTGCTGCAGGGCTGCTTTTTGAGAATGCTGTTGGGTTGCCTGATCCTAATGATGGATCCTTCTATCGGCAGGTTCGACGCCTGCACACAATACTTACCTCTCGGGACTCGATGCAGAATATTCCAGTTAACCTTGAGGCGAGACGCAGAATTGCCTTTTTTAGTAACTCCCTATTTATGAACATACCCCATGCTCCACAGGTTGAGAAAATGATGGCTTTCAGTGTTCTGACCCCGTACTACAGTGAAGAAGTCTTGTACAGCAAAGAACAACTGCGTACTGAGAATGAAGATGGGATTTCTACCCTGTACTATCTACAGACAATATATGTTGATGAGTGGAAAAATTTCATGGAGAGGATGCGTCGAGAAGGAATTGCCAATGATGATGAGATATGGACGACGAAGTTGAGGGAACTCAGGCTTTGGGCGTCTTACAGAGGACAGACACTTACTCGAACTGTAAGGGGGATGATGTATTATTTTCGGGCTCTTAAGATGTTGGCTTTTCTGGATTCTGCATCAGAGATGGACATTCGGGAAGGTTCACAAGAAGTTGGTTCTATGATGCGAGACATTGGTTTGGATGGTTTGACCTCAGAGAGGTCACCTTCTTCCAGGAGTTTAAGTAGAACAAGCAGTTGTGTGAACTCGTTGTACAAAGGTCATGAAGTTGGAACTGCTTTGATGAAATATACATATGTGGTTGCATGCCAGATATATGGAACACAAAAGGCAAAGAAAGATCCCCATGCTGATGAAATTTTGTATCTGATGAAAACCAATGAAGCACTTCGGATTGCCTATGTTGACGAGGTTTCAACTGGCAGGGATGAGAAGGAATATTATTCTGTTCTTGTCAAGTATGATAATCAATTAGAGAAAGAAGTGGAAATCTATCGCATTAAGTTGCCTGGTCCCTTGAAGCTCGGAGAGGGGAAACCAGAGAATCAAAATCATGCCATTATCTTCACTCGTGGTGATGCGGTTCAGACTATTGATATGAACCAAGACAATTATTTTGAGGAGGCACTCAAAATGCGGAATCTATTGGAAGAATACAGGCGCTATTATGGTATCCGGAAGCCTACTATCTTGGGAGTTAGGGAGCATGTCTTTACTGGTTCTGTCTCATCACTTGCTTGGTTTATGTCAGCTCAGGAAACAAGTTTTGTCACCTTGGGACAGCGGGTGTTGGCAAACCCTTTGAAAATTCGAATGCATTATGGTCATCCAGATGTCTTTGACCGATTTTGGTTCTTGACTCGAGGTGGCATCAGTAAAGCTTCCAGGGTGATTAACATCAGTGAAGACATTTTTGCTGGCTTTAATTGCACTTTGCGTGGAGGCAACGTCACCCACCATGAATACATCCAAGTTGGCAAGGGAAGGGATGTTGGGTTTAATCAAATCTCCATGTTTGAGGCCAAGGTTGCTAGTGGAAACGGGGAGCAAGTTCTGAGCAGAGATGTGTATAGGTTGGGCCATAGGCTTGATTTCTTGCGGATGTTATCATTCTTTTATACTACAGTGGGTTTCTTTTTCAACACAATGATGGTGATTTTAACTGTGTATGCCTTTCTATGGGGCCGACTTTATCTGGCTCTCAGTGGTATTGAAGGTTCTATATTGGGGGATGATACCAGTAACAGAGCACTTGGTACAGTCTTGAATCAGCAGTTTATTATCCAGCTTGGTCTGTTCACTGCCCTTCCAATGATAGTGGAGAATTCTCTTGAGCATGGGTTTCTCCAAGCTATCTGGGATTTCTTGACAATGCAGCTCCAGCTTTCTTCAGTTTTCTACACATTTTCAATGGGAACTCGTACCCACTATTTTGGGAGAACCATTCTTCATGGTGGGGCAAAATATCGAGCGACTGGACGTGGCTTTGTTGTGCAGCACAAGAGTTTTGCGGAGAATTATAGACTCTATGCCCGTAGCCATTTTGTGAAAGCAATTGAACTTGGTTTGATACTTACAGTTTATGCGGCATACAGTCCCGTGGCTAAGGACACATTTGTTTACATAGCAATGACCATCACAAGTTGGTTTATGGTGTTGTCCTGGTTTATGGCTCCCTTCGTTTTCAATCCTTCTGGCTTTGATTGGTTGAAGACTGTTGATGATTTTGATGACTTTATGAACTGGATTTGGTATCGTGGCAGTGTGTTTGCCAAAGCTGAACAGAGCTGGGAAAGATGGTGGTATGAGGAGCAGGATCATCTAAGGACAACCGGCGTCTGGGGAAAGTTGCTGGAGATAATTTTAGATCTTCGCTTCTTCTTTTTCCAGTATGGGATCGTATACCAGCTTGGTATTGCTGATAACAGTACAAGTATACTTGTTTACTTGTTATCTTGGATCTATGTGTTCCTGGCTTTTGGCATCTTTATCGTAATAGTGTATGCTCGGGTAAAATATGCAGCAAAAGATCACATTTACTACCGACTGGTCCAATTTCTTGTCATTAAACTTGCACTACTCGTGATAATTGCCCTGTTGGAATTCACAAACTTCAGGTTCATGGATATATTCACCAGTCTTCTGGCATTCATCCCTACTGGTTGGGGTTTGATATTGATAGCACAAGTATTCCGCCCCTTACTACAGCGCACTATACTCTGGGAGATTGTTGTTTCTGTGGCTCGACTGTATGATATATTGTTCGGGGTGATTGTATTGACTCCTGTGGCAGTATTATCATGGTTTCCCGGTTTCCAGTCTATGCAGACTAGGATACTGTTTAATGACGCATTCAGCAGGGGTCTTCGTATATTCCAGATCGTTACAGGAAAAAAGAAGTCCAAGAAAGATTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0005911 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0009506 GO:0009555 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009653 GO:0009719 GO:0009725 GO:0009751 GO:0009863 GO:0009870 GO:0009965 GO:0009987 GO:0010016 GO:0010033 GO:0010150 GO:0012505 GO:0014070 GO:0016020 GO:0016043 GO:0023052 GO:0030054 GO:0031347 GO:0031349 GO:0032501 GO:0032502 GO:0032870 GO:0033036 GO:0033037 GO:0033554 GO:0035690 GO:0042221 GO:0042493 GO:0042545 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045089 GO:0045229 GO:0046677 GO:0048229 GO:0048366 GO:0048367 GO:0048518 GO:0048583 GO:0048584 GO:0048731 GO:0048827 GO:0048856 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051179 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0055044 GO:0065007 GO:0070727 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071407 GO:0071446 GO:0071495 GO:0071554 GO:0071555 GO:0071840 GO:0071944 GO:0080134 GO:0090693 GO:0098542 GO:0099402 GO:1901700 GO:1901701 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FKS1_dom1 Glucan_synthase

Protein Analysis

1771

Amino Acids

206.27

Weight (kDa)

8.88

Isoelectric Point (pI)

36.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FKS1_dom1	PF14288	156 - 268	8.2e-45	1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1	PF25968	681 - 864	1.1e-31	Callose synthase 1 domain
Glucan_synthase	PF02364	872 - 962	2.2e-30	1,3-beta-glucan synthase component
Glucan_synthase	PF02364	968 - 1662	4e-233	1,3-beta-glucan synthase component

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0014998)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G03550
fragaria_vesca	FvH4_6g26200
malus_domestica	MD09G1247400.v1.1 MD17G1240300.v1.1
prunus_persica	Prupe.3G138400_v2.0.a1
pyrus_communis	pycom17g24360
rosa_chinensis	RchiOBHm_Chr2g0126481
rosa_laevigata	RLG00000018906
rosa_multiflora	Rmu_sc0000662.1_g000007
rosa_roxburghii	Rroxscaffold_2G00117140
rosa_rugosa	Rorug02G0263500
rosa_samantha	Rh2AG320100 Rh2BG328800 Rh2CG308500 Rh2DG348100
rosa_wichuraiana	Rw2G025950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1884, 2207
AasI	GACNNNNNNGTC	1 cut(s)	1013
AatII	GACGTC	1 cut(s)	1011
Acc16I	TGCGCA	2 cut(s)	347, 1704
Acc36I	ACCTGC	1 cut(s)	2554
AccB1I	GGYRCC	1 cut(s)	335
AccB7I	CCANNNNNTGG	1 cut(s)	417
AccI	GTMKAC	2 cut(s)	4800, 4834
AccII	CGCG	6 cut(s)	123, 144, 186, 441, 465, 950
AccIII	TCCGGA	2 cut(s)	246, 3573
AcoI	YGGCCR	2 cut(s)	561, 1405
AcsI	RAATTY	6 cut(s)	2011, 2839, 3255, 3705, 4222, 5000
AcuI	CTGAAG	5 cut(s)	926, 1939, 2381, 4272, 4996
AcvI	CACGTG	1 cut(s)	1041
AcyI	GRCGYC	6 cut(s)	336, 444, 1008, 2572, 2924, 4734
AdeI	CACNNNGTG	1 cut(s)	731
AflII	CTTAAG	1 cut(s)	2987
AjiI	CACGTC	1 cut(s)	4379
AjuI	GAANNNNNNNTTGG	4 cut(s)	506, 538, 1469, 1501
Alw21I	GWGCWC	5 cut(s)	264, 392, 471, 1026, 4150
Alw26I	GTCTC	5 cut(s)	674, 1103, 2629, 3060, 3628
AlwNI	CAGNNNCTG	1 cut(s)	2154
Ama87I	CYCGRG	5 cut(s)	113, 530, 2595, 3759, 4905
Aor13HI	TCCGGA	2 cut(s)	246, 3573
ApeKI	GCWGC	9 cut(s)	705, 816, 1151, 1154, 2498, 2506, 4273, 4393, 4919
ApoI	RAATTY	6 cut(s)	2011, 2839, 3255, 3705, 4222, 5000
ArsI	GACNNNNNNTTYG	2 cut(s)	3497, 3529
Asp700I	GAANNNNTTC	3 cut(s)	647, 2840, 3285
AsuC2I	CCSGG	2 cut(s)	44, 5208
AsuII	TTCGAA	3 cut(s)	786, 1720, 3709
AvaI	CYCGRG	5 cut(s)	113, 530, 2595, 3759, 4905
AvaII	GGWCC	6 cut(s)	118, 196, 882, 1200, 3410, 4948
BaeGI	GKGCMC	1 cut(s)	531
BalI	TGGCCA	1 cut(s)	563
BamHI	GGATCC	1 cut(s)	2549
BanI	GGYRCC	1 cut(s)	335
BanII	GRGCYC	4 cut(s)	57, 471, 1122, 2986
BauI	CACGAG	2 cut(s)	3468, 4979
BbrPI	CACGTG	1 cut(s)	1041
BbsI	GAAGAC	6 cut(s)	866, 2484, 3810, 4616, 5030, 5255
Bbv12I	GWGCWC	5 cut(s)	264, 392, 471, 1026, 4150
BbvCI	CCTCAGC	2 cut(s)	1233, 1859
BbvI	GCAGC	9 cut(s)	717, 828, 1138, 1141, 2485, 2493, 4285, 4405, 4931
BceAI	ACGGC	4 cut(s)	131, 323, 349, 1392
BcgI	CGANNNNNNTGC	2 cut(s)	4870, 4904
BciVI	GTATCC	4 cut(s)	17, 990, 3581, 5226
BclI	TGATCA	2 cut(s)	2260, 2334
BcnI	CCSGG	2 cut(s)	44, 5208
BcoDI	GTCTC	5 cut(s)	674, 1103, 2629, 3060, 3628
BfaI	CTAG	3 cut(s)	806, 3930, 5229
BfmI	CTRYAG	6 cut(s)	1061, 2499, 2734, 2810, 4020, 5102
BfoI	RGCGCY	3 cut(s)	339, 2155, 3562
BfrI	CTTAAG	1 cut(s)	2987
BfuAI	ACCTGC	1 cut(s)	2554
BfuI	GTATCC	4 cut(s)	17, 990, 3581, 5226
BglII	AGATCT	2 cut(s)	1650, 4765
BmcAI	AGTACT	1 cut(s)	764
Bme18I	GGWCC	6 cut(s)	118, 196, 882, 1200, 3410, 4948
BmeT110I	CYCGRG	5 cut(s)	113, 530, 2595, 3759, 4905
BmgBI	CACGTC	1 cut(s)	4379
BmiI	GGNNCC	8 cut(s)	167, 251, 337, 1202, 2551, 3412, 4082, 4572
BpiI	GAAGAC	6 cut(s)	866, 2484, 3810, 4616, 5030, 5255
BpmI	CTGGAG	3 cut(s)	605, 4262, 4772
Bpu10I	CCTNAGC	5 cut(s)	1233, 1290, 1859, 3651, 4503
Bpu14I	TTCGAA	3 cut(s)	786, 1720, 3709
BpuEI	CTTGAG	5 cut(s)	779, 1435, 1913, 2648, 4250
BpuMI	CCSGG	2 cut(s)	44, 5208
BsaAI	YACGTR	1 cut(s)	1041
BsaBI	GATNNNNATC	1 cut(s)	3794
BsaHI	GRCGYC	6 cut(s)	336, 444, 1008, 2572, 2924, 4734
BsaI	GGTCTC	1 cut(s)	674
BsaWI	WCCGGW	2 cut(s)	246, 3573
Bse118I	RCCGGY	1 cut(s)	4730
Bse3DI	GCAATG	3 cut(s)	1146, 2065, 4533
Bse8I	GATNNNNATC	1 cut(s)	3794
BseAI	TCCGGA	2 cut(s)	246, 3573
BseJI	GATNNNNATC	1 cut(s)	3794
BseMI	GCAATG	3 cut(s)	1146, 2065, 4533
BseSI	GKGCMC	1 cut(s)	531
BseXI	GCAGC	9 cut(s)	717, 828, 1138, 1141, 2485, 2493, 4285, 4405, 4931
BseYI	CCCAGC	1 cut(s)	4686
BsgI	GTGCAG	2 cut(s)	2561, 4412
Bsh1236I	CGCG	6 cut(s)	123, 144, 186, 441, 465, 950
BshNI	GGYRCC	1 cut(s)	335
BsiHKAI	GWGCWC	5 cut(s)	264, 392, 471, 1026, 4150
BsiHKCI	CYCGRG	5 cut(s)	113, 530, 2595, 3759, 4905
BsiSI	CCGG	8 cut(s)	10, 26, 44, 247, 311, 3574, 4731, 5208
BslFI	GGGAC	9 cut(s)	730, 1019, 1186, 1505, 1828, 2612, 3396, 3690, 4479
BsmAI	GTCTC	5 cut(s)	674, 1103, 2629, 3060, 3628
BsmBI	CGTCTC	1 cut(s)	2629
BsmFI	GGGAC	9 cut(s)	730, 1019, 1186, 1505, 1828, 2612, 3396, 3690, 4479
BsmI	GAATGC	6 cut(s)	649, 2227, 2524, 3717, 5045, 5249
Bso31I	GGTCTC	1 cut(s)	674
BsoBI	CYCGRG	5 cut(s)	113, 530, 2595, 3759, 4905
Bsp119I	TTCGAA	3 cut(s)	786, 1720, 3709
Bsp1286I	GDGCHC	9 cut(s)	57, 264, 392, 471, 531, 1026, 1122, 2986, 4150
Bsp13I	TCCGGA	2 cut(s)	246, 3573
Bsp1407I	TGTACA	2 cut(s)	2751, 3150
Bsp19I	CCATGG	1 cut(s)	564
BspEI	TCCGGA	2 cut(s)	246, 3573
BspFNI	CGCG	6 cut(s)	123, 144, 186, 441, 465, 950
BspHI	TCATGA	1 cut(s)	3160
BspLI	GGNNCC	8 cut(s)	167, 251, 337, 1202, 2551, 3412, 4082, 4572
BspMAI	CTGCAG	2 cut(s)	1065, 2503
BspMI	ACCTGC	1 cut(s)	2554
BspQI	GCTCTTC	2 cut(s)	1785, 2217
BspT104I	TTCGAA	3 cut(s)	786, 1720, 3709
BspT107I	GGYRCC	1 cut(s)	335
BspTI	CTTAAG	1 cut(s)	2987
BspTNI	GGTCTC	1 cut(s)	674
BsrDI	GCAATG	3 cut(s)	1146, 2065, 4533
BsrFI	RCCGGY	1 cut(s)	4730
BsrGI	TGTACA	2 cut(s)	2751, 3150
BssAI	RCCGGY	1 cut(s)	4730
BssNAI	GTATAC	2 cut(s)	4801, 4835
BssNI	GRCGYC	6 cut(s)	336, 444, 1008, 2572, 2924, 4734
BssSI	CACGAG	2 cut(s)	3468, 4979
BssT1I	CCWWGG	5 cut(s)	564, 993, 1322, 3672, 3921
Bst1107I	GTATAC	2 cut(s)	4801, 4835
Bst2BI	CACGAG	2 cut(s)	3468, 4979
Bst6I	CTCTTC	2 cut(s)	1785, 2217
BstACI	GRCGYC	6 cut(s)	336, 444, 1008, 2572, 2924, 4734
BstAFI	CTTAAG	1 cut(s)	2987
BstAUI	TGTACA	2 cut(s)	2751, 3150
BstBAI	YACGTR	1 cut(s)	1041
BstBI	TTCGAA	3 cut(s)	786, 1720, 3709
BstDSI	CCRYGG	3 cut(s)	564, 1408, 4497
BstEII	GGTNACC	1 cut(s)	3099
BstENI	CCTNNNNNAGG	1 cut(s)	3094
BstFNI	CGCG	6 cut(s)	123, 144, 186, 441, 465, 950
BstH2I	RGCGCY	3 cut(s)	339, 2155, 3562
BstMAI	GTCTC	5 cut(s)	674, 1103, 2629, 3060, 3628
BstNSI	RCATGY	4 cut(s)	854, 1648, 3207, 3608
BstPI	GGTNACC	1 cut(s)	3099
BstSFI	CTRYAG	6 cut(s)	1061, 2499, 2734, 2810, 4020, 5102
BstSLI	GKGCMC	1 cut(s)	531
BstUI	CGCG	6 cut(s)	123, 144, 186, 441, 465, 950
BstV1I	GCAGC	9 cut(s)	717, 828, 1138, 1141, 2485, 2493, 4285, 4405, 4931
BstV2I	GAAGAC	6 cut(s)	866, 2484, 3810, 4616, 5030, 5255
BstX2I	RGATCY	6 cut(s)	420, 1650, 2549, 3238, 4765, 4857
BstXI	CCANNNNNNTGG	3 cut(s)	3215, 4331, 5041
BstYI	RGATCY	6 cut(s)	420, 1650, 2549, 3238, 4765, 4857
BstZ17I	GTATAC	2 cut(s)	4801, 4835
BsuI	GTATCC	4 cut(s)	17, 990, 3581, 5226
BtgI	CCRYGG	3 cut(s)	564, 1408, 4497
BtrI	CACGTC	1 cut(s)	4379
BtsI	GCAGTG	3 cut(s)	2304, 4197, 4666
BveI	ACCTGC	1 cut(s)	2554
CaiI	CAGNNNCTG	1 cut(s)	2154
CciI	TCATGA	1 cut(s)	3160
Cfr10I	RCCGGY	1 cut(s)	4730
CseI	GACGC	7 cut(s)	452, 2580, 2646, 2846, 2913, 4723, 5255
CsiI	ACCWGGT	1 cut(s)	2379
CspCI	CAANNNNNGTGG	4 cut(s)	2364, 2399, 4197, 4232
DinI	GGCGCC	1 cut(s)	337
DraI	TTTAAA	1 cut(s)	2299
DraIII	CACNNNGTG	1 cut(s)	731
DrdI	GACNNNNNNGTC	1 cut(s)	1013
DseDI	GACNNNNNNGTC	1 cut(s)	1013
EaeI	YGGCCR	2 cut(s)	561, 1405
Eam1104I	CTCTTC	2 cut(s)	1785, 2217
EarI	CTCTTC	2 cut(s)	1785, 2217
EciI	GGCGGA	4 cut(s)	297, 707, 1372, 5081
Ecl136II	GAGCTC	1 cut(s)	469
Eco130I	CCWWGG	5 cut(s)	564, 993, 1322, 3672, 3921
Eco147I	AGGCCT	2 cut(s)	992, 1425
Eco24I	GRGCYC	4 cut(s)	57, 471, 1122, 2986
Eco31I	GGTCTC	1 cut(s)	674
Eco32I	GATATC	1 cut(s)	754
Eco47I	GGWCC	6 cut(s)	118, 196, 882, 1200, 3410, 4948
Eco53kI	GAGCTC	1 cut(s)	469
Eco57I	CTGAAG	5 cut(s)	926, 1939, 2381, 4272, 4996
Eco72I	CACGTG	1 cut(s)	1041
Eco88I	CYCGRG	5 cut(s)	113, 530, 2595, 3759, 4905
Eco91I	GGTNACC	1 cut(s)	3099
EcoICRI	GAGCTC	1 cut(s)	469
EcoNI	CCTNNNNNAGG	1 cut(s)	3094
EcoO109I	RGGNCCY	2 cut(s)	165, 882
EcoO65I	GGTNACC	1 cut(s)	3099
EcoRI	GAATTC	3 cut(s)	2011, 4222, 5000
EcoRV	GATATC	1 cut(s)	754
EcoT14I	CCWWGG	5 cut(s)	564, 993, 1322, 3672, 3921
EcoT22I	ATGCAT	1 cut(s)	3717
EcoT38I	GRGCYC	4 cut(s)	57, 471, 1122, 2986
EgeI	GGCGCC	1 cut(s)	337
EheI	GGCGCC	1 cut(s)	337
ErhI	CCWWGG	5 cut(s)	564, 993, 1322, 3672, 3921
Esp3I	CGTCTC	1 cut(s)	2629
FaqI	GGGAC	9 cut(s)	730, 1019, 1186, 1505, 1828, 2612, 3396, 3690, 4479
FauI	CCCGC	3 cut(s)	249, 1239, 3675
FauNDI	CATATG	1 cut(s)	3193
FbaI	TGATCA	2 cut(s)	2260, 2334
FblI	GTMKAC	2 cut(s)	4800, 4834
FriOI	GRGCYC	4 cut(s)	57, 471, 1122, 2986
FspBI	CTAG	3 cut(s)	806, 3930, 5229
FspI	TGCGCA	2 cut(s)	347, 1704
GsaI	CCCAGC	1 cut(s)	4690
GsuI	CTGGAG	3 cut(s)	605, 4262, 4772
HaeII	RGCGCY	3 cut(s)	339, 2155, 3562
HapII	CCGG	8 cut(s)	10, 26, 44, 247, 311, 3574, 4731, 5208
HgaI	GACGC	7 cut(s)	452, 2580, 2646, 2846, 2913, 4723, 5255
Hin1I	GRCGYC	6 cut(s)	336, 444, 1008, 2572, 2924, 4734
HincII	GTYRAC	2 cut(s)	2623, 3304
HindII	GTYRAC	2 cut(s)	2623, 3304
HindIII	AAGCTT	3 cut(s)	1889, 2169, 3777
HpaI	GTTAAC	1 cut(s)	2623
HpaII	CCGG	8 cut(s)	10, 26, 44, 247, 311, 3574, 4731, 5208
Hpy99I	CGWCG	7 cut(s)	152, 335, 446, 482, 2574, 2862, 2899
HpyCH4IV	ACGT	6 cut(s)	243, 477, 1008, 1040, 3846, 4378
HpySE526I	ACGT	6 cut(s)	243, 477, 1008, 1040, 3846, 4378
Hsp92I	GRCGYC	6 cut(s)	336, 444, 1008, 2572, 2924, 4734
KasI	GGCGCC	1 cut(s)	335
Kpn2I	TCCGGA	2 cut(s)	246, 3573
Ksp22I	TGATCA	2 cut(s)	2260, 2334
KspAI	GTTAAC	1 cut(s)	2623
LguI	GCTCTTC	2 cut(s)	1785, 2217
Lsp1109I	GCAGC	9 cut(s)	717, 828, 1138, 1141, 2485, 2493, 4285, 4405, 4931
MabI	ACCWGGT	1 cut(s)	2379
MaeI	CTAG	3 cut(s)	806, 3930, 5229
MaeII	ACGT	6 cut(s)	243, 477, 1008, 1040, 3846, 4378
MaeIII	GTNAC	9 cut(s)	829, 1490, 2236, 2657, 3099, 3667, 3847, 4139, 5281
MfeI	CAATTG	3 cut(s)	1733, 2232, 4452
MflI	RGATCY	6 cut(s)	420, 1650, 2549, 3238, 4765, 4857
MhlI	GDGCHC	9 cut(s)	57, 264, 392, 471, 531, 1026, 1122, 2986, 4150
MlsI	TGGCCA	1 cut(s)	563
MluNI	TGGCCA	1 cut(s)	563
Mly113I	GGCGCC	1 cut(s)	336
MlyI	GAGTC	7 cut(s)	1134, 1904, 2089, 2594, 3751, 4416, 5173
MmeI	TCCRAC	6 cut(s)	435, 450, 1753, 1923, 3148, 4977
Mox20I	TGGCCA	1 cut(s)	563
Mph1103I	ATGCAT	1 cut(s)	3717
MroI	TCCGGA	2 cut(s)	246, 3573
MroXI	GAANNNNTTC	3 cut(s)	647, 2840, 3285
MscI	TGGCCA	1 cut(s)	563
MslI	CAYNNNNRTG	6 cut(s)	563, 1710, 3249, 3729, 4046, 4526
Msp20I	TGGCCA	1 cut(s)	563
MspA1I	CMGCKG	2 cut(s)	256, 3682
MspCI	CTTAAG	1 cut(s)	2987
MspI	CCGG	8 cut(s)	10, 26, 44, 247, 311, 3574, 4731, 5208
MunI	CAATTG	3 cut(s)	1733, 2232, 4452
Mva1269I	GAATGC	6 cut(s)	649, 2227, 2524, 3717, 5045, 5249
MvnI	CGCG	6 cut(s)	123, 144, 186, 441, 465, 950
NarI	GGCGCC	1 cut(s)	336
NciI	CCSGG	2 cut(s)	44, 5208
NcoI	CCATGG	1 cut(s)	564
NdeI	CATATG	1 cut(s)	3193
NlaIV	GGNNCC	8 cut(s)	167, 251, 337, 1202, 2551, 3412, 4082, 4572
NmuCI	GTSAC	5 cut(s)	829, 2236, 3099, 3667, 3847
NsbI	TGCGCA	2 cut(s)	347, 1704
NsiI	ATGCAT	1 cut(s)	3717
NspI	RCATGY	4 cut(s)	854, 1648, 3207, 3608
NspV	TTCGAA	3 cut(s)	786, 1720, 3709
PaeI	GCATGC	1 cut(s)	3207
PaeR7I	CTCGAG	1 cut(s)	3759
PagI	TCATGA	1 cut(s)	3160
PceI	AGGCCT	2 cut(s)	992, 1425
PciSI	GCTCTTC	2 cut(s)	1785, 2217
PcsI	WCGNNNNNNNCGW	4 cut(s)	327, 336, 450, 2566
PctI	GAATGC	6 cut(s)	649, 2227, 2524, 3717, 5045, 5249
PdmI	GAANNNNTTC	3 cut(s)	647, 2840, 3285
PflFI	GACNNNGTC	2 cut(s)	242, 1436
PflMI	CCANNNNNTGG	1 cut(s)	417
PfoI	TCCNGGA	2 cut(s)	1160, 3110
PleI	GAGTC	7 cut(s)	1133, 1903, 2088, 2594, 3751, 4416, 5173
PluTI	GGCGCC	1 cut(s)	339
PmaCI	CACGTG	1 cut(s)	1041
PmlI	CACGTG	1 cut(s)	1041
PpsI	GAGTC	7 cut(s)	1133, 1903, 2088, 2594, 3751, 4416, 5173
Ppu21I	YACGTR	1 cut(s)	1041
PpuMI	RGGWCCY	1 cut(s)	882
PsiI	TTATAA	2 cut(s)	1884, 2207
Psp124BI	GAGCTC	1 cut(s)	471
Psp5II	RGGWCCY	1 cut(s)	882
PspCI	CACGTG	1 cut(s)	1041
PspEI	GGTNACC	1 cut(s)	3099
PspFI	CCCAGC	1 cut(s)	4686
PspN4I	GGNNCC	8 cut(s)	167, 251, 337, 1202, 2551, 3412, 4082, 4572
PspPPI	RGGWCCY	1 cut(s)	882
PspXI	VCTCGAGB	1 cut(s)	3759
PsrI	GAACNNNNNNTAC	6 cut(s)	1436, 1468, 3134, 3166, 4099, 4131
PstI	CTGCAG	2 cut(s)	1065, 2503
PstNI	CAGNNNCTG	1 cut(s)	2154
PsuI	RGATCY	6 cut(s)	420, 1650, 2549, 3238, 4765, 4857
PsyI	GACNNNGTC	2 cut(s)	242, 1436
RseI	CAYNNNNRTG	6 cut(s)	563, 1710, 3249, 3729, 4046, 4526
SacI	GAGCTC	1 cut(s)	471
SapI	GCTCTTC	2 cut(s)	1785, 2217
ScaI	AGTACT	1 cut(s)	764
SchI	GAGTC	7 cut(s)	1134, 1904, 2089, 2594, 3751, 4416, 5173
SduI	GDGCHC	9 cut(s)	57, 264, 392, 471, 531, 1026, 1122, 2986, 4150
SexAI	ACCWGGT	1 cut(s)	2379
SfcI	CTRYAG	6 cut(s)	1061, 2499, 2734, 2810, 4020, 5102
SfoI	GGCGCC	1 cut(s)	337
Sfr274I	CTCGAG	1 cut(s)	3759
SfuI	TTCGAA	3 cut(s)	786, 1720, 3709
SinI	GGWCC	6 cut(s)	118, 196, 882, 1200, 3410, 4948
SlaI	CTCGAG	1 cut(s)	3759
SmiMI	CAYNNNNRTG	6 cut(s)	563, 1710, 3249, 3729, 4046, 4526
SmlI	CTYRAG	7 cut(s)	794, 1414, 1892, 2627, 2987, 3759, 4229
SmoI	CTYRAG	7 cut(s)	794, 1414, 1892, 2627, 2987, 3759, 4229
SphI	GCATGC	1 cut(s)	3207
SseBI	AGGCCT	2 cut(s)	992, 1425
SspDI	GGCGCC	1 cut(s)	335
SspI	AATATT	3 cut(s)	1947, 2614, 3338
SspMI	CTAG	3 cut(s)	806, 3930, 5229
SstI	GAGCTC	1 cut(s)	471
StuI	AGGCCT	2 cut(s)	992, 1425
StyI	CCWWGG	5 cut(s)	564, 993, 1322, 3672, 3921
TaiI	ACGT	6 cut(s)	246, 480, 1011, 1043, 3849, 4381
TaqII	GACCGA	1 cut(s)	3756
TatI	WGTACW	7 cut(s)	762, 1443, 2145, 2751, 2802, 3150, 4826
TseFI	GTSAC	5 cut(s)	829, 2236, 3099, 3667, 3847
TseI	GCWGC	9 cut(s)	705, 816, 1151, 1154, 2498, 2506, 4273, 4393, 4919
Tsp45I	GTSAC	5 cut(s)	829, 2236, 3099, 3667, 3847
TspGWI	ACGGA	3 cut(s)	619, 2471, 2478
Tth111I	GACNNNGTC	2 cut(s)	242, 1436
Van91I	CCANNNNNTGG	1 cut(s)	417
Vha464I	CTTAAG	1 cut(s)	2987
VpaK11BI	GGWCC	6 cut(s)	118, 196, 882, 1200, 3410, 4948
XagI	CCTNNNNNAGG	1 cut(s)	3094
XapI	RAATTY	6 cut(s)	2011, 2839, 3255, 3705, 4222, 5000
XbaI	TCTAGA	1 cut(s)	805
XceI	RCATGY	4 cut(s)	854, 1648, 3207, 3608
XcmI	CCANNNNNNNNNTGG	3 cut(s)	732, 1111, 2013
XhoI	CTCGAG	1 cut(s)	3759
XmiI	GTMKAC	2 cut(s)	4800, 4834
XmnI	GAANNNNTTC	3 cut(s)	647, 2840, 3285
XspI	CTAG	3 cut(s)	806, 3930, 5229
ZraI	GACGTC	1 cut(s)	1009
ZrmI	AGTACT	1 cut(s)	764
Zsp2I	ATGCAT	1 cut(s)	3717

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.