FvH4_6g26671

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0018779
Physical Location & Seq
Reverse (-)
20359419 .. 20361483
2065 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g26671.t1

Sequence Viewer

Length: 1044 bp
ATGACACCAGAGGAGGCTTGGAGTGGTGAGAAACCATCTGTAGAACATCTCAGAGTGTTTGGTTGTGTGGGGCATGTTCACATTGTAGATGCTAGGAGGACAAAACTTGAGAACAAGAGTGTTAGCTGTGTACTTCTTGGTGTGAGTGATGAATCCAAGGGCTACAGACTGTATGATCCTATTAGGAAGAAGATTATCGTGAGCCGAGATGTCATATTTGAAGAACAAAGTCAGTGGAACTGGGATTCAAGCTACAGAGAAGAGATAATGATGGATTTAGAGTGGGGAGACAATGAGAATGATGTTGTTATAGAAGAAGAAAATGAAGCTGAAAGGATTGATGAAGCTGAGGTAGCTGATGGTGTAGCTGATGAAGGAACATCAAATGGAGCTGAAGAAGAAGACATTAGAGCAAGTGAGGGCAGAGAAAGACAGCCACCACCATATTTGCAAGATAATGTTTCAGGTGAAGGCCTTTCTGAAAGTGAAGATGTGGTGAATATGGTCCTAGTTGATTCCACAGATCCTATAAACTTTGAAGACGCTAGAGGAGGAGCTGAGAAGTTAGTGGCATTCACTGAGAGTGACTATGCCGGAGATTTGGAGGGAAGAAAGAGCACTTCTGGATATGTGTTTTTGATGAATGATGCTGCTGTTGCACGGTCTTCAAAAAAACAACCTATTGTCACTCTCTCGACAACTGAAGCTGAATTTATTGCAGCGGCTGGTTGTGCCTGTCAAGCAATTTGGATGAGGAGAATTCTGTCTGAAATTGGGTACACACAAGAAGGGAGTACAACTATAATGTGTGATAGTTCTTCTGCCATAAAGTTGTCTAAAAATCCAGTAATGCACGGCCGGAGCAAACATATTGATGTGAGGTTTCACTTCCTGAGAGATCTCACCAAAGAAGGTGTTGTTGAATTGGTGCATTGCGGAACTCATGACCAGATTGCAGATGTGTTGACGAAGCCATTAAAATTGGAGGTTTTTCGTGGACTTCGTGAGCAGCTTGGTGTCTGTGAAGTACCTGTTTTAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

348

Amino Acids

38.62

Weight (kDa)

4.64

Isoelectric Point (pI)

46.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_retrovirus PF25597 22 - 84 2.4e-23 Retroviral polymerase SH3-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 722, 936
AclWI GGATC 2 cut(s) 170, 518
AcoI YGGCCR 1 cut(s) 856
AcsI RAATTY 2 cut(s) 710, 759
AcuI CTGAAG 2 cut(s) 414, 723
AfaI GTAC 4 cut(s) 132, 779, 796, 1029
AgsI TTSAA 5 cut(s) 221, 249, 539, 669, 923
Alw21I GWGCWC 1 cut(s) 620
Alw26I GTCTC 1 cut(s) 282
AlwI GGATC 2 cut(s) 170, 518
AlwNI CAGNNNCTG 1 cut(s) 725
AoxI GGCC 2 cut(s) 472, 856
ApeKI GCWGC 3 cut(s) 650, 719, 1009
ApoI RAATTY 2 cut(s) 710, 759
AspS9I GGNCC 1 cut(s) 505
AsuHPI GGTGA 4 cut(s) 38, 479, 508, 895
AvaII GGWCC 1 cut(s) 505
BbsI GAAGAC 3 cut(s) 408, 546, 657
Bbv12I GWGCWC 1 cut(s) 620
BbvCI CCTCAGC 1 cut(s) 348
BbvI GCAGC 3 cut(s) 637, 731, 1021
BccI CCATC 3 cut(s) 43, 265, 353
BceAI ACGGC 1 cut(s) 871
BcoDI GTCTC 1 cut(s) 282
BfaI CTAG 3 cut(s) 93, 509, 546
BfmI CTRYAG 3 cut(s) 39, 163, 253
BglII AGATCT 1 cut(s) 898
BisI GCNGC 4 cut(s) 651, 720, 723, 1010
BlsI GCNGC 4 cut(s) 652, 721, 724, 1011
Bme18I GGWCC 1 cut(s) 505
BmgT120I GGNCC 1 cut(s) 505
BmrI ACTGGG 1 cut(s) 250
BmsI GCATC 2 cut(s) 79, 637
BmuI ACTGGG 1 cut(s) 250
BpiI GAAGAC 3 cut(s) 408, 546, 657
Bpu10I CCTNAGC 1 cut(s) 348
BpuEI CTTGAG 1 cut(s) 128
BsaJI CCNNGG 1 cut(s) 156
Bse1I ACTGG 2 cut(s) 245, 845
Bse3DI GCAATG 1 cut(s) 931
BseDI CCNNGG 1 cut(s) 156
BseGI GGATG 1 cut(s) 756
BseMI GCAATG 1 cut(s) 931
BseMII CTCAG 5 cut(s) 64, 339, 549, 570, 884
BseNI ACTGG 2 cut(s) 245, 845
BseRI GAGGAG 4 cut(s) 26, 564, 567, 769
BseX3I CGGCCG 1 cut(s) 856
BseXI GCAGC 3 cut(s) 637, 731, 1021
Bsh1285I CGRYCG 1 cut(s) 859
BshFI GGCC 2 cut(s) 474, 858
BsiEI CGRYCG 1 cut(s) 859
BsiHKAI GWGCWC 1 cut(s) 620
BsiSI CCGG 2 cut(s) 594, 859
BsmAI GTCTC 1 cut(s) 282
BsmI GAATGC 1 cut(s) 572
BsnI GGCC 2 cut(s) 474, 858
Bsp1286I GDGCHC 1 cut(s) 620
Bsp143I GATC 3 cut(s) 175, 523, 898
BspACI CCGC 2 cut(s) 722, 936
BspANI GGCC 2 cut(s) 474, 858
BspCNI CTCAG 5 cut(s) 63, 340, 550, 571, 885
BspHI TCATGA 1 cut(s) 943
BspPI GGATC 2 cut(s) 170, 518
BsrDI GCAATG 1 cut(s) 931
BsrI ACTGG 2 cut(s) 245, 845
BssECI CCNNGG 1 cut(s) 156
BssMI GATC 3 cut(s) 175, 523, 898
BssT1I CCWWGG 1 cut(s) 156
Bst4CI ACNGT 2 cut(s) 171, 663
Bst6I CTCTTC 1 cut(s) 255
BstDEI CTNAG 5 cut(s) 50, 348, 558, 579, 893
BstF5I GGATG 1 cut(s) 756
BstKTI GATC 3 cut(s) 178, 526, 901
BstMAI GTCTC 1 cut(s) 282
BstMBI GATC 3 cut(s) 175, 523, 898
BstMCI CGRYCG 1 cut(s) 859
BstMWI GCNNNNNNNGC 4 cut(s) 353, 656, 731, 740
BstNSI RCATGY 1 cut(s) 77
BstSFI CTRYAG 3 cut(s) 39, 163, 253
BstV1I GCAGC 3 cut(s) 637, 731, 1021
BstV2I GAAGAC 3 cut(s) 408, 546, 657
BstX2I RGATCY 2 cut(s) 523, 898
BstYI RGATCY 2 cut(s) 523, 898
BstZI CGGCCG 1 cut(s) 856
BsuRI GGCC 2 cut(s) 474, 858
BtsCI GGATG 1 cut(s) 756
BtsIMutI CAGTG 2 cut(s) 239, 576
CaiI CAGNNNCTG 1 cut(s) 725
CciI TCATGA 1 cut(s) 943
Cfr13I GGNCC 1 cut(s) 505
CseI GACGC 1 cut(s) 551
Csp6I GTAC 4 cut(s) 131, 778, 795, 1028
CspCI CAANNNNNGTGG 4 cut(s) 215, 250, 429, 464
CviAII CATG 2 cut(s) 74, 944
CviQI GTAC 4 cut(s) 131, 778, 795, 1028
DdeI CTNAG 5 cut(s) 50, 348, 558, 579, 893
DpnI GATC 3 cut(s) 177, 525, 900
DpnII GATC 3 cut(s) 175, 523, 898
DraI TTTAAA 1 cut(s) 1038
EaeI YGGCCR 1 cut(s) 856
EagI CGGCCG 1 cut(s) 856
Eam1104I CTCTTC 1 cut(s) 255
EarI CTCTTC 1 cut(s) 255
EclXI CGGCCG 1 cut(s) 856
Eco130I CCWWGG 1 cut(s) 156
Eco147I AGGCCT 1 cut(s) 474
Eco47I GGWCC 1 cut(s) 505
Eco52I CGGCCG 1 cut(s) 856
Eco57I CTGAAG 2 cut(s) 414, 723
EcoRI GAATTC 1 cut(s) 759
EcoT14I CCWWGG 1 cut(s) 156
ErhI CCWWGG 1 cut(s) 156
FaeI CATG 2 cut(s) 77, 947
FatI CATG 2 cut(s) 73, 943
Fnu4HI GCNGC 4 cut(s) 651, 720, 723, 1010
FokI GGATG 1 cut(s) 763
Fsp4HI GCNGC 4 cut(s) 651, 720, 723, 1010
FspBI CTAG 3 cut(s) 93, 509, 546
GluI GCNGC 4 cut(s) 651, 720, 723, 1010
HaeIII GGCC 2 cut(s) 474, 858
HapII CCGG 2 cut(s) 594, 859
HgaI GACGC 1 cut(s) 551
Hin1II CATG 2 cut(s) 77, 947
HincII GTYRAC 1 cut(s) 966
HindII GTYRAC 1 cut(s) 966
HinfI GANTC 3 cut(s) 152, 245, 515
HpaII CCGG 2 cut(s) 594, 859
HphI GGTGA 4 cut(s) 38, 479, 508, 895
Hpy166II GTNNAC 5 cut(s) 79, 131, 780, 966, 998
Hpy188I TCNGA 3 cut(s) 53, 481, 769
Hpy188III TCNNGA 6 cut(s) 199, 624, 694, 892, 944, 1004
Hpy8I GTNNAC 5 cut(s) 79, 131, 780, 966, 998
HpyAV CCTTC 4 cut(s) 368, 464, 782, 905
HpyCH4III ACNGT 2 cut(s) 171, 663
HpyCH4V TGCA 6 cut(s) 451, 659, 719, 853, 931, 956
HpyF10VI GCNNNNNNNGC 4 cut(s) 353, 656, 731, 740
HpyF3I CTNAG 5 cut(s) 50, 348, 558, 579, 893
Hsp92II CATG 2 cut(s) 77, 947
Kzo9I GATC 3 cut(s) 175, 523, 898
LmnI GCTCC 3 cut(s) 389, 554, 861
Lsp1109I GCAGC 3 cut(s) 637, 731, 1021
LweI GCATC 2 cut(s) 79, 637
MaeI CTAG 3 cut(s) 93, 509, 546
MaeIII GTNAC 2 cut(s) 584, 685
MalI GATC 3 cut(s) 177, 525, 900
MboI GATC 3 cut(s) 175, 523, 898
MflI RGATCY 2 cut(s) 523, 898
MhlI GDGCHC 1 cut(s) 620
MluCI AATT 6 cut(s) 710, 744, 759, 771, 923, 980
MseI TTAA 2 cut(s) 977, 1037
MslI CAYNNNNRTG 1 cut(s) 873
MspA1I CMGCKG 1 cut(s) 722
MspI CCGG 2 cut(s) 594, 859
Mva1269I GAATGC 1 cut(s) 572
MwoI GCNNNNNNNGC 4 cut(s) 353, 656, 731, 740
NdeII GATC 3 cut(s) 175, 523, 898
NlaIII CATG 2 cut(s) 77, 947
NmeAIII GCCGAG 1 cut(s) 230
NmuCI GTSAC 2 cut(s) 584, 685
NspI RCATGY 1 cut(s) 77
PagI TCATGA 1 cut(s) 943
PceI AGGCCT 1 cut(s) 474
PcsI WCGNNNNNNNCGW 1 cut(s) 1000
PctI GAATGC 1 cut(s) 572
PfeI GAWTC 3 cut(s) 152, 245, 515
PkrI GCNGC 4 cut(s) 652, 721, 724, 1011
PspPI GGNCC 1 cut(s) 505
PstNI CAGNNNCTG 1 cut(s) 725
PsuI RGATCY 2 cut(s) 523, 898
RsaI GTAC 4 cut(s) 132, 779, 796, 1029
RsaNI GTAC 4 cut(s) 131, 778, 795, 1028
RseI CAYNNNNRTG 1 cut(s) 873
SaqAI TTAA 2 cut(s) 977, 1037
SatI GCNGC 4 cut(s) 651, 720, 723, 1010
Sau3AI GATC 3 cut(s) 175, 523, 898
Sau96I GGNCC 1 cut(s) 505
SduI GDGCHC 1 cut(s) 620
SfaNI GCATC 2 cut(s) 79, 637
SfcI CTRYAG 3 cut(s) 39, 163, 253
SinI GGWCC 1 cut(s) 505
SmiMI CAYNNNNRTG 1 cut(s) 873
SmlI CTYRAG 1 cut(s) 107
SmoI CTYRAG 1 cut(s) 107
Sse9I AATT 6 cut(s) 710, 744, 759, 771, 923, 980
SseBI AGGCCT 1 cut(s) 474
SsiI CCGC 2 cut(s) 722, 936
SspMI CTAG 3 cut(s) 93, 509, 546
StuI AGGCCT 1 cut(s) 474
StyI CCWWGG 1 cut(s) 156
TaaI ACNGT 2 cut(s) 171, 663
TaqI TCGA 1 cut(s) 695
TasI AATT 6 cut(s) 710, 744, 759, 771, 923, 980
TatI WGTACW 2 cut(s) 130, 794
TauI GCSGC 1 cut(s) 725
TfiI GAWTC 3 cut(s) 152, 245, 515
Tru1I TTAA 2 cut(s) 977, 1037
Tru9I TTAA 2 cut(s) 977, 1037
TscAI CASTG 2 cut(s) 239, 583
TseFI GTSAC 2 cut(s) 584, 685
TseI GCWGC 3 cut(s) 650, 719, 1009
Tsp45I GTSAC 2 cut(s) 584, 685
TspDTI ATGAA 5 cut(s) 165, 339, 357, 387, 656
TspRI CASTG 2 cut(s) 239, 583
VpaK11BI GGWCC 1 cut(s) 505
XapI RAATTY 2 cut(s) 710, 759
XceI RCATGY 1 cut(s) 77
XcmI CCANNNNNNNNNTGG 1 cut(s) 15
XspI CTAG 3 cut(s) 93, 509, 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.