FvH4_6g28900

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0016890
Physical Location & Seq
Reverse (-)
22330678 .. 22331370
693 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g28900.t1

Sequence Viewer

Length: 693 bp
ATGTCATTTTCAAGCTGGCTTTGCACCAAGAGCACCAAGAGCATGCTAGTAAAAATTGTTCACCCCGGTGGGCACGTTGAACTTCACGATTGTACCATTCTTGCCGCAGAGCTCATGCATCGGAATCCGAGATGTTGTGTTGCCTATCCACACATCTTCAAGCAGCCGTGGGCGATTGTTTCACCGGACACCATGCTACAGCTAGGCCAGAAGTACTATGTCGTGCCCATAAGTACCATCCGAAAGCTTCAACGCCACTCCTTAAAGTACTCGCCTACTTCGACTCAAGAATGTGAAAGCAAGATCACATCACCATGCAAAGAGGAGGAAGTTGATCATCAAGACATGGTCAAAACTTGTTGGTTTTTCATGAATAAAAACTCTCCCAAGTTACCATATGCTTGTTTGGGACATTCTAGCAGTGACCAAAAAAATGAGGAAAAGGGCTGTTTTCAGAGTAATTACTGCTTTGTATGTTTGCTTATGAGAACCGAGATTAGCGGTGATGATTCAAAAGAGGAAGCAAAATCATCACCGAGCACCAGTGCTTCTACTAGTTCTTCAGAAACCAAGGGACTGACAAGGAAAAGGGCCGGCAGAGATTTTACAAGAATTGGGGTAAAAGGAACACCTAAGAGACTTACATCTCTTGATAATTGGCACCCAAATCTGGAGAGCATCACTGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

231

Amino Acids

26.0

Weight (kDa)

8.59

Isoelectric Point (pI)

63.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 6 - 229 3.4e-22 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016890)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g28900
malus_domestica MD17G1220000.v1.1
prunus_persica Prupe.3G095700_v2.0.a1
pyrus_communis pycom17g22490
rosa_laevigata RLG00000019340
rosa_multiflora Rmu_co8401069.1_g000001 Rmu_sc0016125.1_g000001 Rmu_sc0017323.1_g000012
rosa_rugosa Rorug02G0312000
rosa_samantha Rh2AG364800 Rh2BG370500 Rh2CG348000 Rh2DG387400
rosa_wichuraiana Rw2G029730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 660
AciI CCGC 2 cut(s) 105, 501
AcuI CTGAAG 1 cut(s) 546
AfaI GTAC 4 cut(s) 94, 215, 235, 269
AfiI CCNNNNNNNGG 1 cut(s) 670
AgsI TTSAA 5 cut(s) 12, 80, 160, 251, 513
AhlI ACTAGT 1 cut(s) 554
AleI CACNNNNGTG 1 cut(s) 66
AluBI AGCT 4 cut(s) 15, 112, 202, 247
AluI AGCT 4 cut(s) 15, 112, 202, 247
Alw21I GWGCWC 3 cut(s) 35, 114, 542
Alw26I GTCTC 1 cut(s) 631
AoxI GGCC 2 cut(s) 205, 591
ApeKI GCWGC 1 cut(s) 163
AspS9I GGNCC 1 cut(s) 591
AsuC2I CCSGG 1 cut(s) 66
AsuHPI GGTGA 5 cut(s) 53, 174, 303, 515, 525
BaeGI GKGCMC 2 cut(s) 75, 228
BaeI ACNNNNGTAYC 2 cut(s) 76, 109
BanI GGYRCC 1 cut(s) 660
BanII GRGCYC 1 cut(s) 114
Bbv12I GWGCWC 3 cut(s) 35, 114, 542
BbvI GCAGC 1 cut(s) 175
BccI CCATC 1 cut(s) 245
BceAI ACGGC 1 cut(s) 151
BclI TGATCA 1 cut(s) 334
BcnI CCSGG 1 cut(s) 66
BcoDI GTCTC 1 cut(s) 631
BcuI ACTAGT 1 cut(s) 554
BfaI CTAG 4 cut(s) 47, 203, 417, 555
BfmI CTRYAG 1 cut(s) 197
BisI GCNGC 2 cut(s) 105, 164
BlsI GCNGC 2 cut(s) 106, 165
BmcAI AGTACT 2 cut(s) 215, 269
Bme1390I CCNGG 1 cut(s) 66
BmgT120I GGNCC 1 cut(s) 591
BmiI GGNNCC 1 cut(s) 662
BmrFI CCNGG 1 cut(s) 66
BmsI GCATC 2 cut(s) 127, 687
BpmI CTGGAG 1 cut(s) 692
BpuEI CTTGAG 1 cut(s) 270
BpuMI CCSGG 1 cut(s) 66
BsaJI CCNNGG 3 cut(s) 64, 167, 570
BsaWI WCCGGW 1 cut(s) 184
Bsc4I CCNNNNNNNGG 1 cut(s) 670
Bse118I RCCGGY 1 cut(s) 593
Bse1I ACTGG 1 cut(s) 543
BseDI CCNNGG 3 cut(s) 64, 167, 570
BseGI GGATG 1 cut(s) 237
BseLI CCNNNNNNNGG 1 cut(s) 670
BseNI ACTGG 1 cut(s) 543
BseRI GAGGAG 1 cut(s) 338
BseSI GKGCMC 2 cut(s) 75, 228
BseXI GCAGC 1 cut(s) 175
BshFI GGCC 2 cut(s) 207, 593
BshNI GGYRCC 1 cut(s) 660
BsiHKAI GWGCWC 3 cut(s) 35, 114, 542
BsiSI CCGG 3 cut(s) 66, 185, 594
BslFI GGGAC 2 cut(s) 423, 588
BslI CCNNNNNNNGG 1 cut(s) 670
BsmAI GTCTC 1 cut(s) 631
BsmFI GGGAC 2 cut(s) 423, 588
BsnI GGCC 2 cut(s) 207, 593
Bsp1286I GDGCHC 5 cut(s) 35, 75, 114, 228, 542
Bsp143I GATC 2 cut(s) 303, 334
BspACI CCGC 2 cut(s) 105, 501
BspANI GGCC 2 cut(s) 207, 593
BspHI TCATGA 1 cut(s) 369
BspLI GGNNCC 1 cut(s) 662
BspT107I GGYRCC 1 cut(s) 660
BsrFI RCCGGY 1 cut(s) 593
BsrI ACTGG 1 cut(s) 543
BssAI RCCGGY 1 cut(s) 593
BssECI CCNNGG 3 cut(s) 64, 167, 570
BssMI GATC 2 cut(s) 303, 334
BssT1I CCWWGG 1 cut(s) 570
BstC8I GCNNGC 3 cut(s) 17, 44, 595
BstDEI CTNAG 1 cut(s) 633
BstDSI CCRYGG 1 cut(s) 167
BstF5I GGATG 1 cut(s) 237
BstKTI GATC 2 cut(s) 306, 337
BstMAI GTCTC 1 cut(s) 631
BstMBI GATC 2 cut(s) 303, 334
BstMWI GCNNNNNNNGC 3 cut(s) 21, 30, 39
BstNSI RCATGY 1 cut(s) 46
BstSCI CCNGG 1 cut(s) 64
BstSFI CTRYAG 1 cut(s) 197
BstSLI GKGCMC 2 cut(s) 75, 228
BstV1I GCAGC 1 cut(s) 175
BsuRI GGCC 2 cut(s) 207, 593
BtgI CCRYGG 1 cut(s) 167
BtsCI GGATG 1 cut(s) 237
BtsI GCAGTG 1 cut(s) 427
BtsIMutI CAGTG 3 cut(s) 427, 550, 681
Cac8I GCNNGC 3 cut(s) 17, 44, 595
CciI TCATGA 1 cut(s) 369
Cfr10I RCCGGY 1 cut(s) 593
Cfr13I GGNCC 1 cut(s) 591
Csp6I GTAC 4 cut(s) 93, 214, 234, 268
CviAII CATG 6 cut(s) 43, 115, 193, 315, 346, 370
CviJI RGCY 9 cut(s) 15, 19, 112, 166, 202, 207, 247, 447, 593
CviKI_1 RGCY 9 cut(s) 15, 19, 112, 166, 202, 207, 247, 447, 593
CviQI GTAC 4 cut(s) 93, 214, 234, 268
DdeI CTNAG 1 cut(s) 633
DpnI GATC 2 cut(s) 305, 336
DpnII GATC 2 cut(s) 303, 334
Ecl136II GAGCTC 1 cut(s) 112
Eco130I CCWWGG 1 cut(s) 570
Eco24I GRGCYC 1 cut(s) 114
Eco53kI GAGCTC 1 cut(s) 112
Eco57I CTGAAG 1 cut(s) 546
EcoICRI GAGCTC 1 cut(s) 112
EcoT14I CCWWGG 1 cut(s) 570
EcoT22I ATGCAT 1 cut(s) 120
EcoT38I GRGCYC 1 cut(s) 114
ErhI CCWWGG 1 cut(s) 570
FaeI CATG 6 cut(s) 46, 118, 196, 318, 349, 373
FaqI GGGAC 2 cut(s) 423, 588
FatI CATG 6 cut(s) 42, 114, 192, 314, 345, 369
FauNDI CATATG 1 cut(s) 397
FbaI TGATCA 1 cut(s) 334
Fnu4HI GCNGC 2 cut(s) 105, 164
FokI GGATG 1 cut(s) 224
FriOI GRGCYC 1 cut(s) 114
Fsp4HI GCNGC 2 cut(s) 105, 164
FspBI CTAG 4 cut(s) 47, 203, 417, 555
GluI GCNGC 2 cut(s) 105, 164
GsuI CTGGAG 1 cut(s) 692
HaeIII GGCC 2 cut(s) 207, 593
HapII CCGG 3 cut(s) 66, 185, 594
Hin1II CATG 6 cut(s) 46, 118, 196, 318, 349, 373
HindIII AAGCTT 1 cut(s) 245
HinfI GANTC 3 cut(s) 124, 283, 509
HpaII CCGG 3 cut(s) 66, 185, 594
HphI GGTGA 5 cut(s) 53, 174, 303, 515, 525
Hpy166II GTNNAC 1 cut(s) 61
Hpy188I TCNGA 5 cut(s) 123, 129, 242, 456, 565
Hpy188III TCNNGA 6 cut(s) 86, 287, 341, 370, 650, 671
Hpy8I GTNNAC 1 cut(s) 61
HpyCH4IV ACGT 1 cut(s) 75
HpyCH4V TGCA 3 cut(s) 24, 118, 318
HpyF10VI GCNNNNNNNGC 3 cut(s) 21, 30, 39
HpyF3I CTNAG 1 cut(s) 633
HpySE526I ACGT 1 cut(s) 75
Hsp92II CATG 6 cut(s) 46, 118, 196, 318, 349, 373
KroI GCCGGC 1 cut(s) 593
KroNI GCCGGC 1 cut(s) 595
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 2 cut(s) 303, 334
LpnPI CCDG 6 cut(s) 79, 198, 221, 556, 607, 656
Lsp1109I GCAGC 1 cut(s) 175
LweI GCATC 2 cut(s) 127, 687
MaeI CTAG 4 cut(s) 47, 203, 417, 555
MaeII ACGT 1 cut(s) 75
MaeIII GTNAC 2 cut(s) 390, 422
MalI GATC 2 cut(s) 305, 336
MboI GATC 2 cut(s) 303, 334
MboII GAAGA 2 cut(s) 148, 552
MhlI GDGCHC 5 cut(s) 35, 75, 114, 228, 542
MluCI AATT 4 cut(s) 54, 460, 612, 655
MlyI GAGTC 1 cut(s) 277
MnlI CCTC 4 cut(s) 316, 319, 430, 511
Mph1103I ATGCAT 1 cut(s) 120
MroNI GCCGGC 1 cut(s) 593
MseI TTAA 1 cut(s) 263
MslI CAYNNNNRTG 2 cut(s) 66, 313
MspI CCGG 3 cut(s) 66, 185, 594
MspR9I CCNGG 1 cut(s) 66
MwoI GCNNNNNNNGC 3 cut(s) 21, 30, 39
NaeI GCCGGC 1 cut(s) 595
NciI CCSGG 1 cut(s) 66
NdeI CATATG 1 cut(s) 397
NdeII GATC 2 cut(s) 303, 334
NgoMIV GCCGGC 1 cut(s) 593
NlaIII CATG 6 cut(s) 46, 118, 196, 318, 349, 373
NlaIV GGNNCC 1 cut(s) 662
NmuCI GTSAC 1 cut(s) 422
NsiI ATGCAT 1 cut(s) 120
NspI RCATGY 1 cut(s) 46
OliI CACNNNNGTG 1 cut(s) 66
PaeI GCATGC 1 cut(s) 46
PagI TCATGA 1 cut(s) 369
PcsI WCGNNNNNNNCGW 1 cut(s) 278
PdiI GCCGGC 1 cut(s) 595
PfeI GAWTC 2 cut(s) 124, 509
PflFI GACNNNGTC 1 cut(s) 347
PkrI GCNGC 2 cut(s) 106, 165
PleI GAGTC 1 cut(s) 277
PpsI GAGTC 1 cut(s) 277
Psp124BI GAGCTC 1 cut(s) 114
PspN4I GGNNCC 1 cut(s) 662
PspPI GGNCC 1 cut(s) 591
PsrI GAACNNNNNNTAC 2 cut(s) 42, 74
PsyI GACNNNGTC 1 cut(s) 347
RsaI GTAC 4 cut(s) 94, 215, 235, 269
RsaNI GTAC 4 cut(s) 93, 214, 234, 268
RseI CAYNNNNRTG 2 cut(s) 66, 313
SacI GAGCTC 1 cut(s) 114
SaqAI TTAA 1 cut(s) 263
SatI GCNGC 2 cut(s) 105, 164
Sau3AI GATC 2 cut(s) 303, 334
Sau96I GGNCC 1 cut(s) 591
ScaI AGTACT 2 cut(s) 215, 269
SchI GAGTC 1 cut(s) 277
ScrFI CCNGG 1 cut(s) 66
SduI GDGCHC 5 cut(s) 35, 75, 114, 228, 542
SetI ASST 6 cut(s) 17, 78, 114, 204, 249, 634
SfaNI GCATC 2 cut(s) 127, 687
SfcI CTRYAG 1 cut(s) 197
SmiMI CAYNNNNRTG 2 cut(s) 66, 313
SmlI CTYRAG 1 cut(s) 285
SmoI CTYRAG 1 cut(s) 285
SpeI ACTAGT 1 cut(s) 554
SphI GCATGC 1 cut(s) 46
Sse9I AATT 4 cut(s) 54, 460, 612, 655
SsiI CCGC 2 cut(s) 105, 501
SspMI CTAG 4 cut(s) 47, 203, 417, 555
SstI GAGCTC 1 cut(s) 114
StyD4I CCNGG 1 cut(s) 64
StyI CCWWGG 1 cut(s) 570
TaiI ACGT 1 cut(s) 78
TaqI TCGA 1 cut(s) 281
TasI AATT 4 cut(s) 54, 460, 612, 655
TatI WGTACW 2 cut(s) 213, 267
TauI GCSGC 1 cut(s) 107
TfiI GAWTC 2 cut(s) 124, 509
Tru1I TTAA 1 cut(s) 263
Tru9I TTAA 1 cut(s) 263
TscAI CASTG 3 cut(s) 427, 550, 688
TseFI GTSAC 1 cut(s) 422
TseI GCWGC 1 cut(s) 163
Tsp45I GTSAC 1 cut(s) 422
TspDTI ATGAA 2 cut(s) 358, 386
TspRI CASTG 3 cut(s) 427, 550, 688
Tth111I GACNNNGTC 1 cut(s) 347
XceI RCATGY 1 cut(s) 46
XspI CTAG 4 cut(s) 47, 203, 417, 555
ZrmI AGTACT 2 cut(s) 215, 269
Zsp2I ATGCAT 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.