Rw2G029730

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0016890
Physical Location & Seq
Reverse (-)
48193840 .. 48194551
712 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G029730.1

Sequence Viewer

Length: 675 bp
ATGTCCCTTTCAAACTGGCTTTGCACTAAGAGCACCAAGAACATGCTCGTAAAAATTGTTCACCCCGGCGGCCACGTGGAACTTCACGATTGTACCGTTCTTGCTGCAGAGCTCATGCATCGGAACCCAAGATGTTGTGTTGCCTATCCACAGATCTTCCAGCAGCCGTGGGCGATCGTTGCACCGGACACCATGCTACAGCTAGGCCAGAAGTACTATGTCGTGCCCATAAGCACCATCCGAAAGCTTCAACGCCACTCCTTAAAGTACTTGCCTTCTTCGACTCAAGAATTTCAAAACAAGATCACATCACCAGACAAAGAAGAGGAAGTTGATCATCAAGACATGGTCAAAACTTGTTGGTTTTTCACGAATAAAAATTCTCCCAAGTTACCATATGCTTGTTTGGGACATTCCAGCAGTGACGAAAACAACGTGAATAAAAATGAGGAGGGCTGTTTCCAGAGCAATTATTGCTTTGATTGCTTGCTAATGGGGACCAAGATTAAAGAAGATGGTGATGATTCAAAAGGGGAAGCAAAATTATCACCGAGCACCAGTGCTCCTACTAGTTCTTCAGAAACAAAGGGACTTGCAAGGAAAAGGGAATGCTCTAAGAGACTTGCATCTCTTGATAATTGGCAACCAAGTCTGGAGATCGTTACTGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

224

Amino Acids

25.31

Weight (kDa)

6.65

Isoelectric Point (pI)

60.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 8 - 223 5.3e-21 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016890)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g28900
malus_domestica MD17G1220000.v1.1
prunus_persica Prupe.3G095700_v2.0.a1
pyrus_communis pycom17g22490
rosa_laevigata RLG00000019340
rosa_multiflora Rmu_co8401069.1_g000001 Rmu_sc0016125.1_g000001 Rmu_sc0017323.1_g000012
rosa_rugosa Rorug02G0312000
rosa_samantha Rh2AG364800 Rh2BG370500 Rh2CG348000 Rh2DG387400
rosa_wichuraiana Rw2G029730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 69
AcoI YGGCCR 1 cut(s) 70
AcsI RAATTY 2 cut(s) 290, 379
AcuI CTGAAG 1 cut(s) 561
AcvI CACGTG 1 cut(s) 76
AfaI GTAC 3 cut(s) 94, 215, 269
AgsI TTSAA 4 cut(s) 12, 251, 296, 528
AhlI ACTAGT 1 cut(s) 569
AluBI AGCT 3 cut(s) 112, 202, 247
AluI AGCT 3 cut(s) 112, 202, 247
Alw21I GWGCWC 4 cut(s) 35, 114, 557, 565
Alw26I GTCTC 1 cut(s) 613
AoxI GGCC 2 cut(s) 70, 205
ApeKI GCWGC 2 cut(s) 104, 163
ApoI RAATTY 2 cut(s) 290, 379
AspS9I GGNCC 1 cut(s) 498
AsuC2I CCSGG 1 cut(s) 66
AsuHPI GGTGA 4 cut(s) 53, 303, 530, 540
AvaII GGWCC 1 cut(s) 498
BaeGI GKGCMC 1 cut(s) 228
BaeI ACNNNNGTAYC 2 cut(s) 76, 109
BanII GRGCYC 1 cut(s) 114
BbrPI CACGTG 1 cut(s) 76
Bbv12I GWGCWC 4 cut(s) 35, 114, 557, 565
BbvI GCAGC 2 cut(s) 91, 175
BccI CCATC 2 cut(s) 245, 509
BceAI ACGGC 1 cut(s) 151
BclI TGATCA 1 cut(s) 334
BcnI CCSGG 1 cut(s) 66
BcoDI GTCTC 1 cut(s) 613
BcuI ACTAGT 1 cut(s) 569
BfaI CTAG 2 cut(s) 203, 570
BfmI CTRYAG 2 cut(s) 105, 197
BglII AGATCT 1 cut(s) 153
BisI GCNGC 3 cut(s) 70, 105, 164
BlsI GCNGC 3 cut(s) 71, 106, 165
BmcAI AGTACT 2 cut(s) 215, 269
Bme1390I CCNGG 1 cut(s) 66
Bme18I GGWCC 1 cut(s) 498
BmgT120I GGNCC 1 cut(s) 498
BmiI GGNNCC 2 cut(s) 125, 499
BmrFI CCNGG 1 cut(s) 66
BmsI GCATC 2 cut(s) 127, 635
BpmI CTGGAG 1 cut(s) 674
BpuEI CTTGAG 1 cut(s) 270
BpuMI CCSGG 1 cut(s) 66
BsaAI YACGTR 1 cut(s) 76
BsaJI CCNNGG 2 cut(s) 64, 167
BsaWI WCCGGW 1 cut(s) 184
BsaXI ACNNNNNCTCC 2 cut(s) 547, 577
Bse1I ACTGG 2 cut(s) 20, 558
BseDI CCNNGG 2 cut(s) 64, 167
BseGI GGATG 1 cut(s) 237
BseNI ACTGG 2 cut(s) 20, 558
BseRI GAGGAG 1 cut(s) 464
BseSI GKGCMC 1 cut(s) 228
BseXI GCAGC 2 cut(s) 91, 175
Bsh1285I CGRYCG 1 cut(s) 177
BshFI GGCC 2 cut(s) 72, 207
BsiEI CGRYCG 1 cut(s) 177
BsiHKAI GWGCWC 4 cut(s) 35, 114, 557, 565
BsiSI CCGG 2 cut(s) 66, 185
BslFI GGGAC 3 cut(s) 423, 511, 603
BsmAI GTCTC 1 cut(s) 613
BsmFI GGGAC 3 cut(s) 423, 511, 603
BsmI GAATGC 1 cut(s) 614
BsnI GGCC 2 cut(s) 72, 207
Bsp1286I GDGCHC 5 cut(s) 35, 114, 228, 557, 565
Bsp143I GATC 5 cut(s) 153, 174, 303, 334, 657
BspACI CCGC 1 cut(s) 69
BspANI GGCC 2 cut(s) 72, 207
BspLI GGNNCC 2 cut(s) 125, 499
BspMAI CTGCAG 1 cut(s) 109
BsrI ACTGG 2 cut(s) 20, 558
BssECI CCNNGG 2 cut(s) 64, 167
BssMI GATC 5 cut(s) 153, 174, 303, 334, 657
Bst4CI ACNGT 1 cut(s) 97
Bst6I CTCTTC 1 cut(s) 318
BstAPI GCANNNNNTGC 1 cut(s) 474
BstBAI YACGTR 1 cut(s) 76
BstC8I GCNNGC 1 cut(s) 488
BstDEI CTNAG 2 cut(s) 27, 615
BstDSI CCRYGG 1 cut(s) 167
BstF5I GGATG 1 cut(s) 237
BstKTI GATC 5 cut(s) 156, 177, 306, 337, 660
BstMAI GTCTC 1 cut(s) 613
BstMBI GATC 5 cut(s) 153, 174, 303, 334, 657
BstMCI CGRYCG 1 cut(s) 177
BstMWI GCNNNNNNNGC 4 cut(s) 30, 179, 474, 483
BstNSI RCATGY 1 cut(s) 46
BstSCI CCNGG 1 cut(s) 64
BstSFI CTRYAG 2 cut(s) 105, 197
BstSLI GKGCMC 1 cut(s) 228
BstV1I GCAGC 2 cut(s) 91, 175
BstX2I RGATCY 1 cut(s) 153
BstYI RGATCY 1 cut(s) 153
BsuRI GGCC 2 cut(s) 72, 207
BtgI CCRYGG 1 cut(s) 167
BtsCI GGATG 1 cut(s) 237
BtsI GCAGTG 1 cut(s) 427
BtsIMutI CAGTG 2 cut(s) 427, 565
Cac8I GCNNGC 1 cut(s) 488
Cfr13I GGNCC 1 cut(s) 498
Csp6I GTAC 3 cut(s) 93, 214, 268
CviAII CATG 4 cut(s) 43, 115, 193, 346
CviJI RGCY 8 cut(s) 19, 72, 112, 166, 202, 207, 247, 456
CviKI_1 RGCY 8 cut(s) 19, 72, 112, 166, 202, 207, 247, 456
CviQI GTAC 3 cut(s) 93, 214, 268
DdeI CTNAG 2 cut(s) 27, 615
DpnI GATC 5 cut(s) 155, 176, 305, 336, 659
DpnII GATC 5 cut(s) 153, 174, 303, 334, 657
EaeI YGGCCR 1 cut(s) 70
Eam1104I CTCTTC 1 cut(s) 318
EarI CTCTTC 1 cut(s) 318
Ecl136II GAGCTC 1 cut(s) 112
Eco24I GRGCYC 1 cut(s) 114
Eco47I GGWCC 1 cut(s) 498
Eco53kI GAGCTC 1 cut(s) 112
Eco57I CTGAAG 1 cut(s) 561
Eco72I CACGTG 1 cut(s) 76
EcoICRI GAGCTC 1 cut(s) 112
EcoT22I ATGCAT 1 cut(s) 120
EcoT38I GRGCYC 1 cut(s) 114
FaeI CATG 4 cut(s) 46, 118, 196, 349
FaiI YATR 8 cut(s) 44, 116, 194, 219, 230, 347, 397, 399
FaqI GGGAC 3 cut(s) 423, 511, 603
FatI CATG 4 cut(s) 42, 114, 192, 345
FauNDI CATATG 1 cut(s) 397
FbaI TGATCA 1 cut(s) 334
Fnu4HI GCNGC 3 cut(s) 70, 105, 164
FokI GGATG 1 cut(s) 224
FriOI GRGCYC 1 cut(s) 114
Fsp4HI GCNGC 3 cut(s) 70, 105, 164
FspBI CTAG 2 cut(s) 203, 570
GluI GCNGC 3 cut(s) 70, 105, 164
GsuI CTGGAG 1 cut(s) 674
HaeIII GGCC 2 cut(s) 72, 207
HapII CCGG 2 cut(s) 66, 185
Hin1II CATG 4 cut(s) 46, 118, 196, 349
HindIII AAGCTT 1 cut(s) 245
HinfI GANTC 2 cut(s) 283, 524
HpaII CCGG 2 cut(s) 66, 185
HphI GGTGA 4 cut(s) 53, 303, 530, 540
Hpy166II GTNNAC 1 cut(s) 61
Hpy188I TCNGA 3 cut(s) 123, 242, 580
Hpy188III TCNNGA 7 cut(s) 86, 287, 341, 370, 463, 632, 653
Hpy8I GTNNAC 1 cut(s) 61
HpyAV CCTTC 1 cut(s) 285
HpyCH4III ACNGT 1 cut(s) 97
HpyCH4IV ACGT 2 cut(s) 75, 435
HpyCH4V TGCA 6 cut(s) 24, 107, 118, 182, 596, 626
HpyF10VI GCNNNNNNNGC 4 cut(s) 30, 179, 474, 483
HpyF3I CTNAG 2 cut(s) 27, 615
HpySE526I ACGT 2 cut(s) 75, 435
Hsp92II CATG 4 cut(s) 46, 118, 196, 349
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 5 cut(s) 153, 174, 303, 334, 657
LmnI GCTCC 1 cut(s) 568
LpnPI CCDG 9 cut(s) 79, 173, 198, 221, 327, 430, 476, 571, 638
Lsp1109I GCAGC 2 cut(s) 91, 175
LweI GCATC 2 cut(s) 127, 635
MaeI CTAG 2 cut(s) 203, 570
MaeII ACGT 2 cut(s) 75, 435
MaeIII GTNAC 3 cut(s) 390, 422, 661
MalI GATC 5 cut(s) 155, 176, 305, 336, 659
MboI GATC 5 cut(s) 153, 174, 303, 334, 657
MboII GAAGA 5 cut(s) 148, 270, 335, 524, 567
MflI RGATCY 1 cut(s) 153
MhlI GDGCHC 5 cut(s) 35, 114, 228, 557, 565
MluCI AATT 6 cut(s) 54, 290, 379, 469, 542, 637
MlyI GAGTC 1 cut(s) 277
MnlI CCTC 3 cut(s) 319, 442, 445
Mph1103I ATGCAT 1 cut(s) 120
MseI TTAA 2 cut(s) 263, 507
MspI CCGG 2 cut(s) 66, 185
MspR9I CCNGG 1 cut(s) 66
Mva1269I GAATGC 1 cut(s) 614
MwoI GCNNNNNNNGC 4 cut(s) 30, 179, 474, 483
NciI CCSGG 1 cut(s) 66
NdeI CATATG 1 cut(s) 397
NdeII GATC 5 cut(s) 153, 174, 303, 334, 657
NlaIII CATG 4 cut(s) 46, 118, 196, 349
NlaIV GGNNCC 2 cut(s) 125, 499
NmuCI GTSAC 1 cut(s) 422
NsiI ATGCAT 1 cut(s) 120
NspI RCATGY 1 cut(s) 46
PcsI WCGNNNNNNNCGW 2 cut(s) 93, 432
PctI GAATGC 1 cut(s) 614
PfeI GAWTC 1 cut(s) 524
PflFI GACNNNGTC 1 cut(s) 347
PkrI GCNGC 3 cut(s) 71, 106, 165
Ple19I CGATCG 1 cut(s) 177
PleI GAGTC 1 cut(s) 277
PmaCI CACGTG 1 cut(s) 76
PmlI CACGTG 1 cut(s) 76
PpsI GAGTC 1 cut(s) 277
Ppu21I YACGTR 1 cut(s) 76
Psp124BI GAGCTC 1 cut(s) 114
PspCI CACGTG 1 cut(s) 76
PspN4I GGNNCC 2 cut(s) 125, 499
PspPI GGNCC 1 cut(s) 498
PsrI GAACNNNNNNTAC 2 cut(s) 42, 74
PstI CTGCAG 1 cut(s) 109
PsuI RGATCY 1 cut(s) 153
PsyI GACNNNGTC 1 cut(s) 347
PvuI CGATCG 1 cut(s) 177
RsaI GTAC 3 cut(s) 94, 215, 269
RsaNI GTAC 3 cut(s) 93, 214, 268
SacI GAGCTC 1 cut(s) 114
SaqAI TTAA 2 cut(s) 263, 507
SatI GCNGC 3 cut(s) 70, 105, 164
Sau3AI GATC 5 cut(s) 153, 174, 303, 334, 657
Sau96I GGNCC 1 cut(s) 498
ScaI AGTACT 2 cut(s) 215, 269
SchI GAGTC 1 cut(s) 277
ScrFI CCNGG 1 cut(s) 66
SduI GDGCHC 5 cut(s) 35, 114, 228, 557, 565
SetI ASST 5 cut(s) 78, 114, 204, 249, 438
SfaNI GCATC 2 cut(s) 127, 635
SfcI CTRYAG 2 cut(s) 105, 197
SinI GGWCC 1 cut(s) 498
SmlI CTYRAG 1 cut(s) 285
SmoI CTYRAG 1 cut(s) 285
SpeI ACTAGT 1 cut(s) 569
Sse9I AATT 6 cut(s) 54, 290, 379, 469, 542, 637
SsiI CCGC 1 cut(s) 69
SspMI CTAG 2 cut(s) 203, 570
SstI GAGCTC 1 cut(s) 114
StyD4I CCNGG 1 cut(s) 64
TaaI ACNGT 1 cut(s) 97
TaiI ACGT 2 cut(s) 78, 438
TaqI TCGA 1 cut(s) 281
TasI AATT 6 cut(s) 54, 290, 379, 469, 542, 637
TatI WGTACW 2 cut(s) 213, 267
TauI GCSGC 1 cut(s) 72
TfiI GAWTC 1 cut(s) 524
Tru1I TTAA 2 cut(s) 263, 507
Tru9I TTAA 2 cut(s) 263, 507
TscAI CASTG 2 cut(s) 427, 565
TseFI GTSAC 1 cut(s) 422
TseI GCWGC 2 cut(s) 104, 163
Tsp45I GTSAC 1 cut(s) 422
TspRI CASTG 2 cut(s) 427, 565
Tth111I GACNNNGTC 1 cut(s) 347
VpaK11BI GGWCC 1 cut(s) 498
XapI RAATTY 2 cut(s) 290, 379
XceI RCATGY 1 cut(s) 46
XspI CTAG 2 cut(s) 203, 570
ZrmI AGTACT 2 cut(s) 215, 269
Zsp2I ATGCAT 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.