MD17G1220000.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0016890
Physical Location & Seq
Forward (+)
26827691 .. 26828398
708 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1220000.v1.1.491

Sequence Viewer

Length: 708 bp
ATGTCGATGTCGACATGGCTCTGCACTAAAAGCACCAAGAACATGCTTGTCAAAATTGTTCACCCCGGAGGGCATGTGGAGCTGCACGATAGTCCTCTTCTCGCAGTGGAGATCATGCTAAGAAATCCGCGATGTTGCGTTGCGCACCCTCACATTTTCCAGCAGCCATGGGCGATTGTGGAGCCGGATACCATGCTCAGTCTAGGGCAAAAGTACTACGTCGTGCCCGTAAGCACGATTCGGAAGCTTCAAAGGCTCTCCATAAAGCATTCACCCACTTCAATTCATGACACTCAAAGAAATGTGACACCCCCACCAAGCAAAGAAGATGAGAAACGTGATGACATTCTTTCTAATTATTGTAATTGGTTTTACGCGAGGAATAAAAAATCTACTAAGTTTTCGTATGCTTGTGTTTGTAACCATGAGGAAAACAATGTGAAGGAGGGAGGATGTTTTTCTGAAGATAATTGCTTTGCTTGCTTTTTTAAGGGGACAAAGATTAAAGAAAATGGCGGCCATCACGATTCGAAAGAGGAAACAGAATCATCAAGCAGCAGCATTTCATCTTCTGAGATTGAGACAAAGACGCTTACAAGGAAGAGGAACAGGAATTCTACAAAGCATTGGGGCAAAGATTCAACACCTAAAAGATTGTCATCCCTAGATCATTGGCAGCCAAGTCTAGAGAGTATCCATGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

236

Amino Acids

26.89

Weight (kDa)

8.52

Isoelectric Point (pI)

60.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 7 - 234 1.8e-21 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016890)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g28900
malus_domestica MD17G1220000.v1.1
prunus_persica Prupe.3G095700_v2.0.a1
pyrus_communis pycom17g22490
rosa_laevigata RLG00000019340
rosa_multiflora Rmu_co8401069.1_g000001 Rmu_sc0016125.1_g000001 Rmu_sc0017323.1_g000012
rosa_rugosa Rorug02G0312000
rosa_samantha Rh2AG364800 Rh2BG370500 Rh2CG348000 Rh2DG387400
rosa_wichuraiana Rw2G029730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 144
AccI GTMKAC 1 cut(s) 11
AccII CGCG 2 cut(s) 130, 377
AciI CCGC 2 cut(s) 128, 516
AcoI YGGCCR 1 cut(s) 517
AcsI RAATTY 1 cut(s) 613
AcuI CTGAAG 1 cut(s) 483
AfaI GTAC 1 cut(s) 215
AgsI TTSAA 3 cut(s) 251, 282, 642
AluBI AGCT 2 cut(s) 82, 247
AluI AGCT 2 cut(s) 82, 247
Alw26I GTCTC 1 cut(s) 575
AoxI GGCC 1 cut(s) 517
ApeKI GCWGC 5 cut(s) 82, 163, 555, 558, 676
ApoI RAATTY 1 cut(s) 613
AspLEI GCGC 1 cut(s) 145
AsuC2I CCSGG 1 cut(s) 66
AsuHPI GGTGA 2 cut(s) 53, 264
AsuII TTCGAA 1 cut(s) 530
BaeGI GKGCMC 1 cut(s) 228
BbvI GCAGC 5 cut(s) 69, 175, 567, 570, 688
BccI CCATC 1 cut(s) 528
BciVI GTATCC 2 cut(s) 181, 704
BcnI CCSGG 1 cut(s) 66
BcoDI GTCTC 1 cut(s) 575
BfaI CTAG 3 cut(s) 203, 665, 686
BfuI GTATCC 2 cut(s) 181, 704
BisI GCNGC 6 cut(s) 83, 164, 517, 556, 559, 677
BlsI GCNGC 6 cut(s) 84, 165, 518, 557, 560, 678
BmcAI AGTACT 1 cut(s) 215
Bme1390I CCNGG 1 cut(s) 66
BmiI GGNNCC 1 cut(s) 183
BmrFI CCNGG 1 cut(s) 66
Bpu14I TTCGAA 1 cut(s) 530
BpuMI CCSGG 1 cut(s) 66
BsaBI GATNNNNATC 1 cut(s) 658
BsaJI CCNNGG 2 cut(s) 64, 167
BsaXI ACNNNNNCTCC 2 cut(s) 60, 90
Bse8I GATNNNNATC 1 cut(s) 658
BseDI CCNNGG 2 cut(s) 64, 167
BseGI GGATG 2 cut(s) 458, 659
BseJI GATNNNNATC 1 cut(s) 658
BseMII CTCAG 2 cut(s) 211, 564
BseSI GKGCMC 1 cut(s) 228
BseXI GCAGC 5 cut(s) 69, 175, 567, 570, 688
BsgI GTGCAG 2 cut(s) 7, 68
Bsh1236I CGCG 2 cut(s) 130, 377
BshFI GGCC 1 cut(s) 519
BsiSI CCGG 2 cut(s) 66, 185
BslFI GGGAC 1 cut(s) 508
BsmAI GTCTC 1 cut(s) 575
BsmFI GGGAC 1 cut(s) 508
BsmI GAATGC 1 cut(s) 268
BsnI GGCC 1 cut(s) 519
Bsp119I TTCGAA 1 cut(s) 530
Bsp1286I GDGCHC 1 cut(s) 228
Bsp143I GATC 2 cut(s) 111, 667
Bsp19I CCATGG 1 cut(s) 167
BspACI CCGC 2 cut(s) 128, 516
BspANI GGCC 1 cut(s) 519
BspCNI CTCAG 2 cut(s) 210, 565
BspFNI CGCG 2 cut(s) 130, 377
BspHI TCATGA 1 cut(s) 286
BspLI GGNNCC 1 cut(s) 183
BspT104I TTCGAA 1 cut(s) 530
BssECI CCNNGG 2 cut(s) 64, 167
BssMI GATC 2 cut(s) 111, 667
BssT1I CCWWGG 1 cut(s) 167
Bst6I CTCTTC 2 cut(s) 102, 596
BstBI TTCGAA 1 cut(s) 530
BstC8I GCNNGC 1 cut(s) 481
BstDEI CTNAG 4 cut(s) 119, 197, 396, 573
BstDSI CCRYGG 1 cut(s) 167
BstF5I GGATG 2 cut(s) 458, 659
BstFNI CGCG 2 cut(s) 130, 377
BstHHI GCGC 1 cut(s) 145
BstKTI GATC 2 cut(s) 114, 670
BstMAI GTCTC 1 cut(s) 575
BstMBI GATC 2 cut(s) 111, 667
BstMWI GCNNNNNNNGC 4 cut(s) 30, 79, 253, 480
BstNSI RCATGY 2 cut(s) 46, 77
BstSCI CCNGG 1 cut(s) 64
BstSLI GKGCMC 1 cut(s) 228
BstUI CGCG 2 cut(s) 130, 377
BstV1I GCAGC 5 cut(s) 69, 175, 567, 570, 688
BsuI GTATCC 2 cut(s) 181, 704
BsuRI GGCC 1 cut(s) 519
BtgI CCRYGG 1 cut(s) 167
BtgZI GCGATG 1 cut(s) 145
BtsCI GGATG 2 cut(s) 458, 659
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 1 cut(s) 111
Cac8I GCNNGC 1 cut(s) 481
CciI TCATGA 1 cut(s) 286
CfoI GCGC 1 cut(s) 145
CseI GACGC 1 cut(s) 598
Csp6I GTAC 1 cut(s) 214
CviAII CATG 9 cut(s) 15, 43, 74, 115, 168, 193, 287, 425, 698
CviJI RGCY 8 cut(s) 19, 82, 166, 184, 247, 256, 519, 679
CviKI_1 RGCY 8 cut(s) 19, 82, 166, 184, 247, 256, 519, 679
CviQI GTAC 1 cut(s) 214
DdeI CTNAG 4 cut(s) 119, 197, 396, 573
DpnI GATC 2 cut(s) 113, 669
DpnII GATC 2 cut(s) 111, 667
EaeI YGGCCR 1 cut(s) 517
Eam1104I CTCTTC 2 cut(s) 102, 596
EarI CTCTTC 2 cut(s) 102, 596
Eco130I CCWWGG 1 cut(s) 167
Eco57I CTGAAG 1 cut(s) 483
EcoRI GAATTC 1 cut(s) 613
EcoT14I CCWWGG 1 cut(s) 167
ErhI CCWWGG 1 cut(s) 167
FaeI CATG 9 cut(s) 18, 46, 77, 118, 171, 196, 290, 428, 701
FaqI GGGAC 1 cut(s) 508
FatI CATG 9 cut(s) 14, 42, 73, 114, 167, 192, 286, 424, 697
FblI GTMKAC 1 cut(s) 11
Fnu4HI GCNGC 6 cut(s) 83, 164, 517, 556, 559, 677
FokI GGATG 2 cut(s) 465, 646
Fsp4HI GCNGC 6 cut(s) 83, 164, 517, 556, 559, 677
FspBI CTAG 3 cut(s) 203, 665, 686
FspI TGCGCA 1 cut(s) 144
GlaI GCGC 1 cut(s) 144
GluI GCNGC 6 cut(s) 83, 164, 517, 556, 559, 677
HaeIII GGCC 1 cut(s) 519
HapII CCGG 2 cut(s) 66, 185
HgaI GACGC 1 cut(s) 598
HhaI GCGC 1 cut(s) 145
Hin1II CATG 9 cut(s) 18, 46, 77, 118, 171, 196, 290, 428, 701
Hin6I GCGC 1 cut(s) 143
HinP1I GCGC 1 cut(s) 143
HincII GTYRAC 1 cut(s) 12
HindII GTYRAC 1 cut(s) 12
HindIII AAGCTT 1 cut(s) 245
HinfI GANTC 4 cut(s) 238, 527, 545, 638
HpaII CCGG 2 cut(s) 66, 185
HphI GGTGA 2 cut(s) 53, 264
Hpy166II GTNNAC 2 cut(s) 12, 61
Hpy188I TCNGA 3 cut(s) 243, 463, 574
Hpy188III TCNNGA 3 cut(s) 287, 524, 686
Hpy8I GTNNAC 2 cut(s) 12, 61
Hpy99I CGWCG 1 cut(s) 224
HpyAV CCTTC 1 cut(s) 436
HpyCH4IV ACGT 2 cut(s) 219, 337
HpyCH4V TGCA 2 cut(s) 24, 85
HpyF10VI GCNNNNNNNGC 4 cut(s) 30, 79, 253, 480
HpyF3I CTNAG 4 cut(s) 119, 197, 396, 573
HpySE526I ACGT 2 cut(s) 219, 337
Hsp92II CATG 9 cut(s) 18, 46, 77, 118, 171, 196, 290, 428, 701
HspAI GCGC 1 cut(s) 143
Kzo9I GATC 2 cut(s) 111, 667
LmnI GCTCC 2 cut(s) 79, 181
LpnPI CCDG 4 cut(s) 79, 173, 198, 595
Lsp1109I GCAGC 5 cut(s) 69, 175, 567, 570, 688
MaeI CTAG 3 cut(s) 203, 665, 686
MaeII ACGT 2 cut(s) 219, 337
MaeIII GTNAC 2 cut(s) 304, 419
MalI GATC 2 cut(s) 113, 669
MboI GATC 2 cut(s) 111, 667
MboII GAAGA 5 cut(s) 89, 338, 476, 561, 613
MhlI GDGCHC 1 cut(s) 228
MluCI AATT 6 cut(s) 54, 282, 355, 364, 469, 613
MnlI CCTC 9 cut(s) 62, 105, 159, 372, 421, 439, 443, 529, 597
MseI TTAA 2 cut(s) 489, 504
MspI CCGG 2 cut(s) 66, 185
MspR9I CCNGG 1 cut(s) 66
Mva1269I GAATGC 1 cut(s) 268
MvnI CGCG 2 cut(s) 130, 377
MwoI GCNNNNNNNGC 4 cut(s) 30, 79, 253, 480
NciI CCSGG 1 cut(s) 66
NcoI CCATGG 1 cut(s) 167
NdeII GATC 2 cut(s) 111, 667
NlaIII CATG 9 cut(s) 18, 46, 77, 118, 171, 196, 290, 428, 701
NlaIV GGNNCC 1 cut(s) 183
NmuCI GTSAC 1 cut(s) 304
NsbI TGCGCA 1 cut(s) 144
NspI RCATGY 2 cut(s) 46, 77
NspV TTCGAA 1 cut(s) 530
PagI TCATGA 1 cut(s) 286
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PctI GAATGC 1 cut(s) 268
PfeI GAWTC 4 cut(s) 238, 527, 545, 638
PkrI GCNGC 6 cut(s) 84, 165, 518, 557, 560, 678
PspN4I GGNNCC 1 cut(s) 183
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
SalI GTCGAC 1 cut(s) 10
SaqAI TTAA 2 cut(s) 489, 504
SatI GCNGC 6 cut(s) 83, 164, 517, 556, 559, 677
Sau3AI GATC 2 cut(s) 111, 667
ScaI AGTACT 1 cut(s) 215
ScrFI CCNGG 1 cut(s) 66
SduI GDGCHC 1 cut(s) 228
SetI ASST 5 cut(s) 84, 222, 249, 340, 649
SfuI TTCGAA 1 cut(s) 530
Sse9I AATT 6 cut(s) 54, 282, 355, 364, 469, 613
SsiI CCGC 2 cut(s) 128, 516
SspMI CTAG 3 cut(s) 203, 665, 686
StyD4I CCNGG 1 cut(s) 64
StyI CCWWGG 1 cut(s) 167
TaiI ACGT 2 cut(s) 222, 340
TaqI TCGA 3 cut(s) 5, 11, 530
TasI AATT 6 cut(s) 54, 282, 355, 364, 469, 613
TatI WGTACW 1 cut(s) 213
TauI GCSGC 1 cut(s) 519
TfiI GAWTC 4 cut(s) 238, 527, 545, 638
Tru1I TTAA 2 cut(s) 489, 504
Tru9I TTAA 2 cut(s) 489, 504
TscAI CASTG 1 cut(s) 111
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 5 cut(s) 82, 163, 555, 558, 676
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 2 cut(s) 275, 555
TspRI CASTG 1 cut(s) 111
XapI RAATTY 1 cut(s) 613
XbaI TCTAGA 1 cut(s) 685
XceI RCATGY 2 cut(s) 46, 77
XmiI GTMKAC 1 cut(s) 11
XspI CTAG 3 cut(s) 203, 665, 686
ZrmI AGTACT 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.