FvH4_6g29440

Hus1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0015085
Physical Location & Seq
Reverse (-)
22738864 .. 22740895
2032 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g29440.t1

Sequence Viewer

Length: 879 bp
ATGAAGTTCAAGGCATTTCTTACTGAGAACGGTGTGAATCTTTTGGAAAAGAGGTTCTTACCAGCACTAGACAAGATGGGGAAGATATGCCATCTCTTTCTCAACAGGGACCGTGTTTTCTTTCTGCACAACCTTCTTAATGGCAATGGAGTTCAGTCCATTGCTCAGTTTAGCAAAGAGGCTCTTTTTGATGACTATCGCATCTCTAGCCAGTATGATGACTGCATTGCCTTTACATTAGACATTTCTCTTCTGCACCGTGCTCTTCGTAGCAGTGTCAGCATATGTACTGAGTTTGGAAATGGGCCCACAGCTAATCGCTTACAGATTAAATTAGCAAAGAAGCTCACTAAGAATTCTACTGAAAGGTTGCCCTTCCTTACCTTTGAGACCAAGGGTTATAAATCTGCAGTCATCCAGGACGTGCCAATCTCATCACCTTTGTCTAGGAATCAGGTTCTTGAACTTCAAACTGCTCTTGATATGGCTCAAGATTTGCCCCAGACTCTGGTGCAGGTTCCAGACTTGAATCAATTGCAAAACTTTGTGGATCGGATGAGGCATGTTGGTGATCTGCTTAATGTTTCTATAAGTGAGCAAGCTGATGCCCCTGCCGAAGACCGAAACCTGAGTGCTCAGACTCGAGCAGCAAGGGCAGTTCTAAGGGGAGATGCTCAATCAGTGCAAGTGAGTGTCAAGCACTTCGGTAAGAGCCTTCAATGCAATTTGGCTAAGCCAGACTGTGCTTTCTATGGGATTGCTCCACAAGGTGCTTGCTTGACACTGGTTTTTCAATTTTTCATTCCTGGTACACATCGACTTGATAAGTCAATCAGTATGCATTGCAGACTTCCTGTACTTGACCAAGGGTCCGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04218 map04218
Pfam Domains
Protein Families
Hus1

Protein Analysis

293

Amino Acids

32.54

Weight (kDa)

8.82

Isoelectric Point (pI)

39.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hus1 PF04005 1 - 200 2.3e-49 Hus1-like protein
Hus1 PF04005 201 - 271 3.5e-08 Hus1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015085)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52530 AT1G52530
fragaria_vesca FvH4_6g29440 FvH4_6g29440
malus_domestica MD17G1214300.v1.1
prunus_persica Prupe.3G090200_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0134931
rosa_laevigata RLG00000019431
rosa_multiflora Rmu_sc0001385.1_g000004 Rmu_sc0007475.1_g000007
rosa_roxburghii Rroxscaffold_2G00109630
rosa_rugosa Rorug02G0321300
rosa_samantha Rh2AG372500 Rh2BG378800 Rh2CG357700
rosa_wichuraiana Rw2G030410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 402
Acc36I ACCTGC 1 cut(s) 505
AccB7I CCANNNNNTGG 1 cut(s) 508
AclWI GGATC 1 cut(s) 558
AcsI RAATTY 1 cut(s) 355
AdeI CACNNNGTG 1 cut(s) 770
AfaI GTAC 3 cut(s) 289, 811, 858
AfiI CCNNNNNNNGG 1 cut(s) 508
AgsI TTSAA 6 cut(s) 10, 464, 470, 529, 719, 794
AhdI GACNNNNNGTC 1 cut(s) 868
AjiI CACGTC 1 cut(s) 424
AjnI CCWGG 2 cut(s) 417, 805
AloI GAACNNNNNNTCC 2 cut(s) 38, 70
AluBI AGCT 3 cut(s) 314, 346, 602
AluI AGCT 3 cut(s) 314, 346, 602
Alw21I GWGCWC 2 cut(s) 265, 637
Alw26I GTCTC 1 cut(s) 383
AlwI GGATC 1 cut(s) 558
AlwNI CAGNNNCTG 1 cut(s) 508
Ama87I CYCGRG 1 cut(s) 642
AoxI GGCC 1 cut(s) 305
ApaI GGGCCC 1 cut(s) 309
ApeKI GCWGC 1 cut(s) 647
ApoI RAATTY 1 cut(s) 355
AspS9I GGNCC 4 cut(s) 109, 305, 306, 870
AsuHPI GGTGA 2 cut(s) 429, 581
AvaI CYCGRG 1 cut(s) 642
AvaII GGWCC 2 cut(s) 109, 870
BaeGI GKGCMC 1 cut(s) 309
BanII GRGCYC 1 cut(s) 309
BbsI GAAGAC 1 cut(s) 624
Bbv12I GWGCWC 2 cut(s) 265, 637
BbvI GCAGC 1 cut(s) 659
BccI CCATC 2 cut(s) 70, 99
BciT130I CCWGG 2 cut(s) 419, 807
BcoDI GTCTC 1 cut(s) 383
BfaI CTAG 3 cut(s) 68, 207, 447
BfmI CTRYAG 1 cut(s) 408
BfuAI ACCTGC 1 cut(s) 505
BisI GCNGC 1 cut(s) 648
BlpI GCTNAGC 1 cut(s) 732
BlsI GCNGC 1 cut(s) 649
Bme1390I CCNGG 2 cut(s) 419, 807
Bme18I GGWCC 2 cut(s) 109, 870
BmeRI GACNNNNNGTC 1 cut(s) 868
BmeT110I CYCGRG 1 cut(s) 642
BmgBI CACGTC 1 cut(s) 424
BmgT120I GGNCC 4 cut(s) 109, 305, 306, 870
BmiI GGNNCC 4 cut(s) 110, 307, 519, 871
BmrFI CCNGG 2 cut(s) 419, 807
BmsI GCATC 3 cut(s) 210, 595, 661
BpiI GAAGAC 1 cut(s) 624
Bpu1102I GCTNAGC 1 cut(s) 732
BpuEI CTTGAG 1 cut(s) 474
BsaBI GATNNNNATC 1 cut(s) 195
BsaI GGTCTC 1 cut(s) 383
BsaJI CCNNGG 2 cut(s) 393, 865
Bsc4I CCNNNNNNNGG 1 cut(s) 508
Bse1I ACTGG 2 cut(s) 211, 789
Bse3DI GCAATG 4 cut(s) 151, 159, 225, 841
Bse8I GATNNNNATC 1 cut(s) 195
BseBI CCWGG 2 cut(s) 419, 807
BseDI CCNNGG 2 cut(s) 393, 865
BseGI GGATG 2 cut(s) 414, 561
BseJI GATNNNNATC 1 cut(s) 195
BseLI CCNNNNNNNGG 1 cut(s) 508
BseMI GCAATG 4 cut(s) 151, 159, 225, 841
BseMII CTCAG 5 cut(s) 15, 179, 282, 620, 650
BseNI ACTGG 2 cut(s) 211, 789
BseSI GKGCMC 1 cut(s) 309
BseXI GCAGC 1 cut(s) 659
BsgI GTGCAG 3 cut(s) 110, 239, 533
BshFI GGCC 1 cut(s) 307
BsiHKAI GWGCWC 2 cut(s) 265, 637
BsiHKCI CYCGRG 1 cut(s) 642
BsiSI CCGG 1 cut(s) 873
BslFI GGGAC 1 cut(s) 122
BslI CCNNNNNNNGG 1 cut(s) 508
BsmAI GTCTC 1 cut(s) 383
BsmFI GGGAC 1 cut(s) 122
BsnI GGCC 1 cut(s) 307
Bso31I GGTCTC 1 cut(s) 383
BsoBI CYCGRG 1 cut(s) 642
Bsp120I GGGCCC 1 cut(s) 305
Bsp1286I GDGCHC 3 cut(s) 265, 309, 637
Bsp143I GATC 2 cut(s) 550, 571
Bsp1720I GCTNAGC 1 cut(s) 732
BspANI GGCC 1 cut(s) 307
BspCNI CTCAG 5 cut(s) 16, 178, 283, 621, 649
BspLI GGNNCC 4 cut(s) 110, 307, 519, 871
BspMAI CTGCAG 1 cut(s) 412
BspMI ACCTGC 1 cut(s) 505
BspPI GGATC 1 cut(s) 558
BspQI GCTCTTC 1 cut(s) 270
BspTNI GGTCTC 1 cut(s) 383
BsrDI GCAATG 4 cut(s) 151, 159, 225, 841
BsrI ACTGG 2 cut(s) 211, 789
BssECI CCNNGG 2 cut(s) 393, 865
BssMI GATC 2 cut(s) 550, 571
BssT1I CCWWGG 2 cut(s) 393, 865
Bst2UI CCWGG 2 cut(s) 419, 807
Bst4CI ACNGT 4 cut(s) 32, 113, 260, 743
Bst6I CTCTTC 2 cut(s) 255, 270
BstC8I GCNNGC 2 cut(s) 600, 775
BstDEI CTNAG 8 cut(s) 24, 165, 291, 351, 629, 636, 662, 732
BstF5I GGATG 2 cut(s) 414, 561
BstKTI GATC 2 cut(s) 553, 574
BstMAI GTCTC 1 cut(s) 383
BstMBI GATC 2 cut(s) 550, 571
BstMWI GCNNNNNNNGC 4 cut(s) 207, 279, 653, 720
BstNI CCWGG 2 cut(s) 419, 807
BstNSI RCATGY 1 cut(s) 566
BstSCI CCNGG 2 cut(s) 417, 805
BstSFI CTRYAG 1 cut(s) 408
BstSLI GKGCMC 1 cut(s) 309
BstV1I GCAGC 1 cut(s) 659
BstV2I GAAGAC 1 cut(s) 624
BsuRI GGCC 1 cut(s) 307
BtrI CACGTC 1 cut(s) 424
BtsCI GGATG 2 cut(s) 414, 561
BtsI GCAGTG 1 cut(s) 280
BtsIMutI CAGTG 3 cut(s) 280, 687, 782
BveI ACCTGC 1 cut(s) 505
Cac8I GCNNGC 2 cut(s) 600, 775
CaiI CAGNNNCTG 1 cut(s) 508
Cfr13I GGNCC 4 cut(s) 109, 305, 306, 870
Csp6I GTAC 3 cut(s) 288, 810, 857
CviAII CATG 1 cut(s) 563
CviQI GTAC 3 cut(s) 288, 810, 857
DdeI CTNAG 8 cut(s) 24, 165, 291, 351, 629, 636, 662, 732
DpnI GATC 2 cut(s) 552, 573
DpnII GATC 2 cut(s) 550, 571
DraIII CACNNNGTG 1 cut(s) 770
DriI GACNNNNNGTC 1 cut(s) 868
Eam1104I CTCTTC 2 cut(s) 255, 270
Eam1105I GACNNNNNGTC 1 cut(s) 868
EarI CTCTTC 2 cut(s) 255, 270
Eco130I CCWWGG 2 cut(s) 393, 865
Eco24I GRGCYC 1 cut(s) 309
Eco31I GGTCTC 1 cut(s) 383
Eco47I GGWCC 2 cut(s) 109, 870
Eco88I CYCGRG 1 cut(s) 642
EcoRI GAATTC 1 cut(s) 355
EcoRII CCWGG 2 cut(s) 417, 805
EcoT14I CCWWGG 2 cut(s) 393, 865
EcoT22I ATGCAT 1 cut(s) 843
EcoT38I GRGCYC 1 cut(s) 309
ErhI CCWWGG 2 cut(s) 393, 865
FaeI CATG 1 cut(s) 566
FalI AAGNNNNNCTT 4 cut(s) 41, 73, 168, 200
FaqI GGGAC 1 cut(s) 122
FatI CATG 1 cut(s) 562
FauNDI CATATG 1 cut(s) 284
Fnu4HI GCNGC 1 cut(s) 648
FokI GGATG 2 cut(s) 401, 568
FriOI GRGCYC 1 cut(s) 309
Fsp4HI GCNGC 1 cut(s) 648
FspBI CTAG 3 cut(s) 68, 207, 447
GluI GCNGC 1 cut(s) 648
HaeIII GGCC 1 cut(s) 307
HapII CCGG 1 cut(s) 873
Hin1II CATG 1 cut(s) 566
HinfI GANTC 5 cut(s) 37, 451, 505, 529, 640
HpaII CCGG 1 cut(s) 873
HphI GGTGA 2 cut(s) 429, 581
Hpy166II GTNNAC 1 cut(s) 812
Hpy188I TCNGA 2 cut(s) 555, 639
Hpy188III TCNNGA 4 cut(s) 461, 479, 491, 521
Hpy8I GTNNAC 1 cut(s) 812
HpyAV CCTTC 3 cut(s) 143, 385, 725
HpyCH4III ACNGT 4 cut(s) 32, 113, 260, 743
HpyCH4IV ACGT 1 cut(s) 423
HpyF10VI GCNNNNNNNGC 4 cut(s) 207, 279, 653, 720
HpyF3I CTNAG 8 cut(s) 24, 165, 291, 351, 629, 636, 662, 732
HpySE526I ACGT 1 cut(s) 423
Hsp92II CATG 1 cut(s) 566
Kzo9I GATC 2 cut(s) 550, 571
LguI GCTCTTC 1 cut(s) 270
LmnI GCTCC 1 cut(s) 766
Lsp1109I GCAGC 1 cut(s) 659
LweI GCATC 3 cut(s) 210, 595, 661
MaeI CTAG 3 cut(s) 68, 207, 447
MaeII ACGT 1 cut(s) 423
MalI GATC 2 cut(s) 552, 573
MboI GATC 2 cut(s) 550, 571
MboII GAAGA 4 cut(s) 94, 242, 257, 629
MfeI CAATTG 1 cut(s) 533
MhlI GDGCHC 3 cut(s) 265, 309, 637
MluCI AATT 5 cut(s) 332, 355, 533, 724, 794
MlyI GAGTC 2 cut(s) 499, 634
MnlI CCTC 3 cut(s) 45, 172, 552
Mph1103I ATGCAT 1 cut(s) 843
MseI TTAA 3 cut(s) 138, 330, 579
MslI CAYNNNNRTG 1 cut(s) 567
MspI CCGG 1 cut(s) 873
MspR9I CCNGG 2 cut(s) 419, 807
MunI CAATTG 1 cut(s) 533
MvaI CCWGG 2 cut(s) 419, 807
MwoI GCNNNNNNNGC 4 cut(s) 207, 279, 653, 720
NdeI CATATG 1 cut(s) 284
NdeII GATC 2 cut(s) 550, 571
NlaIII CATG 1 cut(s) 566
NlaIV GGNNCC 4 cut(s) 110, 307, 519, 871
NsiI ATGCAT 1 cut(s) 843
NspI RCATGY 1 cut(s) 566
PaeR7I CTCGAG 1 cut(s) 642
PciSI GCTCTTC 1 cut(s) 270
PfeI GAWTC 3 cut(s) 37, 451, 529
PflMI CCANNNNNTGG 1 cut(s) 508
PfoI TCCNGGA 1 cut(s) 417
PkrI GCNGC 1 cut(s) 649
PleI GAGTC 2 cut(s) 499, 634
PpsI GAGTC 2 cut(s) 499, 634
PsiI TTATAA 1 cut(s) 402
Psp6I CCWGG 2 cut(s) 417, 805
PspGI CCWGG 2 cut(s) 417, 805
PspN4I GGNNCC 4 cut(s) 110, 307, 519, 871
PspOMI GGGCCC 1 cut(s) 305
PspPI GGNCC 4 cut(s) 109, 305, 306, 870
PspXI VCTCGAGB 1 cut(s) 642
PstI CTGCAG 1 cut(s) 412
PstNI CAGNNNCTG 1 cut(s) 508
RsaI GTAC 3 cut(s) 289, 811, 858
RsaNI GTAC 3 cut(s) 288, 810, 857
RseI CAYNNNNRTG 1 cut(s) 567
SapI GCTCTTC 1 cut(s) 270
SaqAI TTAA 3 cut(s) 138, 330, 579
SatI GCNGC 1 cut(s) 648
Sau3AI GATC 2 cut(s) 550, 571
Sau96I GGNCC 4 cut(s) 109, 305, 306, 870
SchI GAGTC 2 cut(s) 499, 634
ScrFI CCNGG 2 cut(s) 419, 807
SduI GDGCHC 3 cut(s) 265, 309, 637
SfaNI GCATC 3 cut(s) 210, 595, 661
SfcI CTRYAG 1 cut(s) 408
Sfr274I CTCGAG 1 cut(s) 642
SinI GGWCC 2 cut(s) 109, 870
SlaI CTCGAG 1 cut(s) 642
SmiMI CAYNNNNRTG 1 cut(s) 567
SmlI CTYRAG 2 cut(s) 489, 642
SmoI CTYRAG 2 cut(s) 489, 642
Sse9I AATT 5 cut(s) 332, 355, 533, 724, 794
SspMI CTAG 3 cut(s) 68, 207, 447
StyD4I CCNGG 2 cut(s) 417, 805
StyI CCWWGG 2 cut(s) 393, 865
TaaI ACNGT 4 cut(s) 32, 113, 260, 743
TaiI ACGT 1 cut(s) 426
TaqI TCGA 2 cut(s) 643, 817
TaqII GACCGA 1 cut(s) 636
TasI AATT 5 cut(s) 332, 355, 533, 724, 794
TatI WGTACW 2 cut(s) 287, 856
TfiI GAWTC 3 cut(s) 37, 451, 529
Tru1I TTAA 3 cut(s) 138, 330, 579
Tru9I TTAA 3 cut(s) 138, 330, 579
TscAI CASTG 3 cut(s) 280, 687, 789
TseI GCWGC 1 cut(s) 647
TspDTI ATGAA 2 cut(s) 17, 790
TspRI CASTG 3 cut(s) 280, 687, 789
Van91I CCANNNNNTGG 1 cut(s) 508
VpaK11BI GGWCC 2 cut(s) 109, 870
XapI RAATTY 1 cut(s) 355
XceI RCATGY 1 cut(s) 566
XhoI CTCGAG 1 cut(s) 642
XspI CTAG 3 cut(s) 68, 207, 447
Zsp2I ATGCAT 1 cut(s) 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.