MD17G1214300.v1.1

Hus1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0015085
Physical Location & Seq
Forward (+)
26111448 .. 26113841
2394 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1214300.v1.1.491

Sequence Viewer

Length: 960 bp
ATGAAGTTCAAGGCATTTCTTACAGAGAATGGTGTAAATCTTTTGGAAAAGAGGTTCCTACCAGCCCTAGACAAGATGGGAAAGATATGCCATCTCTACCTCAACAGAGACCATGCTATCTTCCTCCACAACCTTCTTAATGGTGATGGAATTCAATCCATTGCCCAGTTTCGCAAAGAAGCTCTTTTTGATGACTATCGCATCTCCAGCCAGAATGAGGACCGCATTGCCTTTACAGTAGACATCTCTCTTCTCCACCGTGCTCTTCGTAGCAGTGTCAGCATATGTACTGAGTTTGGAAATGGTCCGACAGCCAACCGGCTCCAGATTAGATTGGTTAAAAAACTACCTCCAAATTCTACCCAACCATTGCCTTTCCTGACTTTCGAGACCAAGGGTTATAAATCTGCGGTAATTCAGGATGTACCAATCTCAAAACCTTTGTCCAGAGCCCAGGTTCTGGAACTTCAAACTGCTCTTGATGCAGCTCAAGACTTGCCTCAGACTCTGGTTCAGGTTCCCGATCTGAATCAGTTGCAAAATTTTGTGGATCGGATGAGGCATGTTGGTGATTTGCTCAATGTTACCATAAGCAAGTATGGGGATTTACACATTCAGATTTCAACAACACTCATCACACTTGGTGCAGAGTTTCAGAAGCTGTTGGTCATAGGTGAGCAAGCTGATGCTCCATCTGAAGATCGAAATATGACCGCTCAGACTCGGTCGGCAAGGGCTATTTTAAGGGGTGATGCTCAATCTGTGCAAGTGAGTGTCAAGCATTTTGGTAAGAGCCTTCAGTGCCACTTGGCTAAACCGGACTGCGCTTTCTATGGGATTGCCGCACAAGGTGCTTGCTTGACAGTGATCTTCCAATTTTTCATTCCTGGTTCTCATCAAATCGATAAGTCCATCAGTCTACACTGCAGACTTCCTGTACTTGACCAAGGATCGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04218 map04218
Pfam Domains
Protein Families
Hus1

Protein Analysis

320

Amino Acids

35.45

Weight (kDa)

8.7

Isoelectric Point (pI)

35.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hus1 PF04005 1 - 298 1.8e-75 Hus1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015085)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52530 AT1G52530
fragaria_vesca FvH4_6g29440 FvH4_6g29440
malus_domestica MD17G1214300.v1.1
prunus_persica Prupe.3G090200_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0134931
rosa_laevigata RLG00000019431
rosa_multiflora Rmu_sc0001385.1_g000004 Rmu_sc0007475.1_g000007
rosa_roxburghii Rroxscaffold_2G00109630
rosa_rugosa Rorug02G0321300
rosa_samantha Rh2AG372500 Rh2BG378800 Rh2CG357700
rosa_wichuraiana Rw2G030410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 402
AccB7I CCANNNNNTGG 1 cut(s) 460
AccBSI CCGCTC 1 cut(s) 716
AccI GTMKAC 2 cut(s) 240, 919
AciI CCGC 4 cut(s) 223, 410, 714, 843
AclWI GGATC 2 cut(s) 558, 958
AcsI RAATTY 3 cut(s) 150, 355, 541
AcuI CTGAAG 2 cut(s) 717, 782
AdeI CACNNNGTG 2 cut(s) 644, 851
AfaI GTAC 3 cut(s) 289, 426, 939
AfiI CCNNNNNNNGG 3 cut(s) 217, 460, 952
AgsI TTSAA 4 cut(s) 10, 155, 470, 624
AjnI CCWGG 2 cut(s) 453, 886
AjuI GAANNNNNNNTTGG 2 cut(s) 867, 899
AloI GAACNNNNNNTCC 2 cut(s) 38, 70
AluBI AGCT 4 cut(s) 182, 488, 661, 683
AluI AGCT 4 cut(s) 182, 488, 661, 683
Alw21I GWGCWC 1 cut(s) 265
Alw26I GTCTC 2 cut(s) 102, 383
AlwI GGATC 2 cut(s) 558, 958
AlwNI CAGNNNCTG 3 cut(s) 460, 508, 661
ApeKI GCWGC 1 cut(s) 485
ApoI RAATTY 3 cut(s) 150, 355, 541
ArsI GACNNNNNNTTYG 2 cut(s) 428, 460
AspLEI GCGC 1 cut(s) 827
AspS9I GGNCC 2 cut(s) 220, 305
AsuHPI GGTGA 4 cut(s) 155, 581, 686, 761
AvaII GGWCC 2 cut(s) 220, 305
BanII GRGCYC 1 cut(s) 454
Bbv12I GWGCWC 1 cut(s) 265
BbvI GCAGC 1 cut(s) 497
BccI CCATC 5 cut(s) 70, 99, 140, 700, 920
BciT130I CCWGG 2 cut(s) 455, 888
BcoDI GTCTC 2 cut(s) 102, 383
BfaI CTAG 1 cut(s) 68
BfmI CTRYAG 1 cut(s) 925
BisI GCNGC 2 cut(s) 486, 843
BlsI GCNGC 2 cut(s) 487, 844
Bme1390I CCNGG 2 cut(s) 455, 888
Bme18I GGWCC 2 cut(s) 220, 305
BmgT120I GGNCC 2 cut(s) 220, 305
BmiI GGNNCC 3 cut(s) 56, 323, 519
BmrFI CCNGG 2 cut(s) 455, 888
BmrI ACTGGG 1 cut(s) 160
BmsI GCATC 4 cut(s) 210, 472, 676, 742
BmuI ACTGGG 1 cut(s) 160
BpmI CTGGAG 2 cut(s) 190, 308
BpuEI CTTGAG 1 cut(s) 474
Bsa29I ATCGAT 1 cut(s) 903
BsaBI GATNNNNATC 2 cut(s) 195, 528
BsaI GGTCTC 2 cut(s) 102, 383
BsaJI CCNNGG 3 cut(s) 393, 453, 946
BsaWI WCCGGW 1 cut(s) 817
Bsc4I CCNNNNNNNGG 3 cut(s) 217, 460, 952
Bse118I RCCGGY 1 cut(s) 318
Bse1I ACTGG 1 cut(s) 166
Bse3DI GCAATG 3 cut(s) 159, 225, 368
Bse8I GATNNNNATC 2 cut(s) 195, 528
BseBI CCWGG 2 cut(s) 455, 888
BseCI ATCGAT 1 cut(s) 903
BseDI CCNNGG 3 cut(s) 393, 453, 946
BseGI GGATG 2 cut(s) 427, 561
BseJI GATNNNNATC 2 cut(s) 195, 528
BseLI CCNNNNNNNGG 3 cut(s) 217, 460, 952
BseMI GCAATG 3 cut(s) 159, 225, 368
BseMII CTCAG 3 cut(s) 282, 515, 731
BseNI ACTGG 1 cut(s) 166
BseXI GCAGC 1 cut(s) 497
BsgI GTGCAG 1 cut(s) 666
Bsh1285I CGRYCG 1 cut(s) 728
BshVI ATCGAT 1 cut(s) 903
BsiEI CGRYCG 1 cut(s) 728
BsiHKAI GWGCWC 1 cut(s) 265
BsiSI CCGG 2 cut(s) 319, 818
BslI CCNNNNNNNGG 3 cut(s) 217, 460, 952
BsmAI GTCTC 2 cut(s) 102, 383
Bso31I GGTCTC 2 cut(s) 102, 383
Bsp1286I GDGCHC 2 cut(s) 265, 454
Bsp143I GATC 5 cut(s) 523, 550, 700, 867, 950
BspACI CCGC 4 cut(s) 223, 410, 714, 843
BspCNI CTCAG 3 cut(s) 283, 514, 730
BspDI ATCGAT 1 cut(s) 903
BspLI GGNNCC 3 cut(s) 56, 323, 519
BspMAI CTGCAG 1 cut(s) 929
BspPI GGATC 2 cut(s) 558, 958
BspQI GCTCTTC 1 cut(s) 270
BspTNI GGTCTC 2 cut(s) 102, 383
BsrBI CCGCTC 1 cut(s) 716
BsrDI GCAATG 3 cut(s) 159, 225, 368
BsrFI RCCGGY 1 cut(s) 318
BsrI ACTGG 1 cut(s) 166
BssAI RCCGGY 1 cut(s) 318
BssECI CCNNGG 3 cut(s) 393, 453, 946
BssMI GATC 5 cut(s) 523, 550, 700, 867, 950
BssT1I CCWWGG 2 cut(s) 393, 946
Bst2UI CCWGG 2 cut(s) 455, 888
Bst4CI ACNGT 3 cut(s) 238, 260, 865
Bst6I CTCTTC 2 cut(s) 255, 270
BstAPI GCANNNNNTGC 1 cut(s) 851
BstC8I GCNNGC 2 cut(s) 681, 856
BstDEI CTNAG 3 cut(s) 291, 501, 717
BstF5I GGATG 2 cut(s) 427, 561
BstHHI GCGC 1 cut(s) 827
BstKTI GATC 5 cut(s) 526, 553, 703, 870, 953
BstMAI GTCTC 2 cut(s) 102, 383
BstMBI GATC 5 cut(s) 523, 550, 700, 867, 950
BstMCI CGRYCG 1 cut(s) 728
BstMWI GCNNNNNNNGC 5 cut(s) 207, 279, 482, 801, 851
BstNI CCWGG 2 cut(s) 455, 888
BstNSI RCATGY 1 cut(s) 566
BstSCI CCNGG 2 cut(s) 453, 886
BstSFI CTRYAG 1 cut(s) 925
BstV1I GCAGC 1 cut(s) 497
Bsu15I ATCGAT 1 cut(s) 903
BsuTUI ATCGAT 1 cut(s) 903
BtsCI GGATG 2 cut(s) 427, 561
BtsI GCAGTG 2 cut(s) 280, 922
BtsIMutI CAGTG 4 cut(s) 280, 806, 870, 922
Cac8I GCNNGC 2 cut(s) 681, 856
CaiI CAGNNNCTG 3 cut(s) 460, 508, 661
CfoI GCGC 1 cut(s) 827
Cfr10I RCCGGY 1 cut(s) 318
Cfr13I GGNCC 2 cut(s) 220, 305
ClaI ATCGAT 1 cut(s) 903
Csp6I GTAC 3 cut(s) 288, 425, 938
CviAII CATG 2 cut(s) 113, 563
CviQI GTAC 3 cut(s) 288, 425, 938
DdeI CTNAG 3 cut(s) 291, 501, 717
DpnI GATC 5 cut(s) 525, 552, 702, 869, 952
DpnII GATC 5 cut(s) 523, 550, 700, 867, 950
DraIII CACNNNGTG 2 cut(s) 644, 851
Eam1104I CTCTTC 2 cut(s) 255, 270
EarI CTCTTC 2 cut(s) 255, 270
Eco130I CCWWGG 2 cut(s) 393, 946
Eco24I GRGCYC 1 cut(s) 454
Eco31I GGTCTC 2 cut(s) 102, 383
Eco47I GGWCC 2 cut(s) 220, 305
Eco57I CTGAAG 2 cut(s) 717, 782
EcoRI GAATTC 1 cut(s) 150
EcoRII CCWGG 2 cut(s) 453, 886
EcoT14I CCWWGG 2 cut(s) 393, 946
EcoT38I GRGCYC 1 cut(s) 454
ErhI CCWWGG 2 cut(s) 393, 946
FaeI CATG 2 cut(s) 116, 566
FalI AAGNNNNNCTT 2 cut(s) 168, 200
FatI CATG 2 cut(s) 112, 562
FauNDI CATATG 1 cut(s) 284
FblI GTMKAC 2 cut(s) 240, 919
Fnu4HI GCNGC 2 cut(s) 486, 843
FokI GGATG 2 cut(s) 434, 568
FriOI GRGCYC 1 cut(s) 454
Fsp4HI GCNGC 2 cut(s) 486, 843
FspBI CTAG 1 cut(s) 68
GlaI GCGC 1 cut(s) 826
GluI GCNGC 2 cut(s) 486, 843
GsuI CTGGAG 2 cut(s) 190, 308
HapII CCGG 2 cut(s) 319, 818
HhaI GCGC 1 cut(s) 827
Hin1II CATG 2 cut(s) 116, 566
Hin6I GCGC 1 cut(s) 825
HinP1I GCGC 1 cut(s) 825
HinfI GANTC 3 cut(s) 505, 529, 721
HpaII CCGG 2 cut(s) 319, 818
HphI GGTGA 4 cut(s) 155, 581, 686, 761
Hpy166II GTNNAC 2 cut(s) 241, 920
Hpy188I TCNGA 8 cut(s) 309, 504, 528, 555, 618, 657, 697, 720
Hpy188III TCNNGA 9 cut(s) 325, 379, 388, 419, 447, 461, 479, 491, 521
Hpy8I GTNNAC 2 cut(s) 241, 920
HpyAV CCTTC 2 cut(s) 143, 806
HpyCH4III ACNGT 3 cut(s) 238, 260, 865
HpyCH4V TGCA 5 cut(s) 485, 538, 647, 766, 927
HpyF10VI GCNNNNNNNGC 5 cut(s) 207, 279, 482, 801, 851
HpyF3I CTNAG 3 cut(s) 291, 501, 717
Hsp92II CATG 2 cut(s) 116, 566
HspAI GCGC 1 cut(s) 825
Kzo9I GATC 5 cut(s) 523, 550, 700, 867, 950
LguI GCTCTTC 1 cut(s) 270
LmnI GCTCC 2 cut(s) 327, 694
Lsp1109I GCAGC 1 cut(s) 497
LweI GCATC 4 cut(s) 210, 472, 676, 742
MaeI CTAG 1 cut(s) 68
MaeIII GTNAC 1 cut(s) 583
MalI GATC 5 cut(s) 525, 552, 702, 869, 952
MbiI CCGCTC 1 cut(s) 716
MboI GATC 5 cut(s) 523, 550, 700, 867, 950
MboII GAAGA 5 cut(s) 112, 242, 257, 710, 862
MhlI GDGCHC 2 cut(s) 265, 454
MluCI AATT 5 cut(s) 150, 355, 414, 541, 875
MlyI GAGTC 2 cut(s) 499, 715
MmeI TCCRAC 1 cut(s) 332
MnlI CCTC 7 cut(s) 45, 110, 134, 211, 360, 510, 552
MseI TTAA 4 cut(s) 138, 339, 743, 958
MslI CAYNNNNRTG 1 cut(s) 567
MspI CCGG 2 cut(s) 319, 818
MspR9I CCNGG 2 cut(s) 455, 888
MvaI CCWGG 2 cut(s) 455, 888
MwoI GCNNNNNNNGC 5 cut(s) 207, 279, 482, 801, 851
NdeI CATATG 1 cut(s) 284
NdeII GATC 5 cut(s) 523, 550, 700, 867, 950
NlaIII CATG 2 cut(s) 116, 566
NlaIV GGNNCC 3 cut(s) 56, 323, 519
NspI RCATGY 1 cut(s) 566
PciSI GCTCTTC 1 cut(s) 270
PfeI GAWTC 1 cut(s) 529
PflFI GACNNNGTC 1 cut(s) 724
PflMI CCANNNNNTGG 1 cut(s) 460
PkrI GCNGC 2 cut(s) 487, 844
PleI GAGTC 2 cut(s) 499, 715
PpsI GAGTC 2 cut(s) 499, 715
PsiI TTATAA 1 cut(s) 402
Psp6I CCWGG 2 cut(s) 453, 886
PspGI CCWGG 2 cut(s) 453, 886
PspN4I GGNNCC 3 cut(s) 56, 323, 519
PspPI GGNCC 2 cut(s) 220, 305
PstI CTGCAG 1 cut(s) 929
PstNI CAGNNNCTG 3 cut(s) 460, 508, 661
PsyI GACNNNGTC 1 cut(s) 724
RsaI GTAC 3 cut(s) 289, 426, 939
RsaNI GTAC 3 cut(s) 288, 425, 938
RseI CAYNNNNRTG 1 cut(s) 567
SapI GCTCTTC 1 cut(s) 270
SaqAI TTAA 4 cut(s) 138, 339, 743, 958
SatI GCNGC 2 cut(s) 486, 843
Sau3AI GATC 5 cut(s) 523, 550, 700, 867, 950
Sau96I GGNCC 2 cut(s) 220, 305
SchI GAGTC 2 cut(s) 499, 715
ScrFI CCNGG 2 cut(s) 455, 888
SduI GDGCHC 2 cut(s) 265, 454
SfaNI GCATC 4 cut(s) 210, 472, 676, 742
SfcI CTRYAG 1 cut(s) 925
SinI GGWCC 2 cut(s) 220, 305
SmiMI CAYNNNNRTG 1 cut(s) 567
SmlI CTYRAG 1 cut(s) 489
SmoI CTYRAG 1 cut(s) 489
Sse9I AATT 5 cut(s) 150, 355, 414, 541, 875
SsiI CCGC 4 cut(s) 223, 410, 714, 843
SspMI CTAG 1 cut(s) 68
StyD4I CCNGG 2 cut(s) 453, 886
StyI CCWWGG 2 cut(s) 393, 946
TaaI ACNGT 3 cut(s) 238, 260, 865
TaqI TCGA 3 cut(s) 387, 703, 903
TaqII GACCGA 1 cut(s) 714
TasI AATT 5 cut(s) 150, 355, 414, 541, 875
TatI WGTACW 2 cut(s) 287, 937
TauI GCSGC 1 cut(s) 845
TfiI GAWTC 1 cut(s) 529
Tru1I TTAA 4 cut(s) 138, 339, 743, 958
Tru9I TTAA 4 cut(s) 138, 339, 743, 958
TscAI CASTG 4 cut(s) 280, 806, 870, 929
TseI GCWGC 1 cut(s) 485
TspDTI ATGAA 2 cut(s) 17, 871
TspRI CASTG 4 cut(s) 280, 806, 870, 929
Tth111I GACNNNGTC 1 cut(s) 724
Van91I CCANNNNNTGG 1 cut(s) 460
VpaK11BI GGWCC 2 cut(s) 220, 305
XapI RAATTY 3 cut(s) 150, 355, 541
XceI RCATGY 1 cut(s) 566
XmiI GTMKAC 2 cut(s) 240, 919
XspI CTAG 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.