Rh2AG372500

Hus1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0015085
Physical Location & Seq
Forward (+)
55415969 .. 55418084
2116 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG372500.1

Sequence Viewer

Length: 960 bp
ATGAAGTTCAAGGCATTTCTTACAGAGAACGGTGTGAGTCTTTTAGAAAAGAGGTTCTTACCAGCACTAGACAAGATGGGAAAGATATGCCATCTCTTCCTCAACAGGGACCGTGTTTTCTTCCTCCACAACCTTCTCAATGGCGATGGAGTTCAATCCATTGCTCAGTTTAGCAAAGAAGCTCTTTTTGATGATTATCGCATCTCTAGCCAGTACGATGACTGCATTTCCTTTACAGTAGACATTTCTCTTCTGCAGCGTGCTCTTCGTAGCAGTGTCAGCATATGTACTGAGTTTGGAAATGGGCCAACAGCTAATCGCCTGCAGATTAAATTAGCAAAAAAGCTTACTAAAAATTCTACTCAAAGATTGCCTTTCCTTACCTTTGAGACCAAGGGTTATAAATCTGCAGTAATCCAGGATGTACCAATCTCATCACCTTTGTCTAGGAATCAGGTTCTTGAACTTCAAACTGCTCTTGATATGGCTCAAGATTTGCCCCAGACTCTGGTTCAGGTTCCAGATTTGAATCAGTTGCAAAATTTTGTGGATCGGATGAGGCATGTTGGTGATCTTCTTAATATTTCCATAAGCAAGTATGGGGATTTACACATTCAGATTTCAACTACACTGATCACACTTGGTGCTGAGTTTCAGAAGCTTTTGGTCATAGGGGAGCAAGCTGATGCCCCGTCTGAAGACCGAAACTTGAGTGCTCAGACTCGGGCAGCAAGGGCGGTTCTAAGGGGAGATGCTCAATCAGTGCAAGTGAGTGTGAAGCATTTTGGTAAGAGCCTTCAGTGCCATTTGGCTAAGCCAGACTGCGCTTTCTATGGGATTGCTCTAGAAGGTGCTTGCTTGACACTGGTTTTTCAATTTTTCATTCCTGGTACCCATCGACTTGATAAGTCAATCAGTATGCATTGCAGACTTCCTGTACTTGACCAAGGGTCCAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04218 map04218
Pfam Domains
Protein Families
Hus1

Protein Analysis

319

Amino Acids

35.56

Weight (kDa)

8.63

Isoelectric Point (pI)

39.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hus1 PF04005 1 - 298 1.6e-73 Hus1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015085)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52530 AT1G52530
fragaria_vesca FvH4_6g29440 FvH4_6g29440
malus_domestica MD17G1214300.v1.1
prunus_persica Prupe.3G090200_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0134931
rosa_laevigata RLG00000019431
rosa_multiflora Rmu_sc0001385.1_g000004 Rmu_sc0007475.1_g000007
rosa_roxburghii Rroxscaffold_2G00109630
rosa_rugosa Rorug02G0321300
rosa_samantha Rh2AG372500 Rh2BG378800 Rh2CG357700
rosa_wichuraiana Rw2G030410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 402
Acc65I GGTACC 1 cut(s) 890
AccB1I GGYRCC 1 cut(s) 890
AccB7I CCANNNNNTGG 1 cut(s) 508
AccI GTMKAC 1 cut(s) 240
AciI CCGC 1 cut(s) 737
AclWI GGATC 1 cut(s) 558
AcsI RAATTY 2 cut(s) 355, 541
AcuI CTGAAG 2 cut(s) 717, 782
AdeI CACNNNGTG 1 cut(s) 644
AfaI GTAC 5 cut(s) 215, 289, 426, 892, 939
AfiI CCNNNNNNNGG 2 cut(s) 106, 508
AgsI TTSAA 7 cut(s) 10, 155, 464, 470, 529, 624, 875
AhdI GACNNNNNGTC 1 cut(s) 949
AjnI CCWGG 2 cut(s) 417, 886
AluBI AGCT 6 cut(s) 182, 314, 346, 661, 683, 957
AluI AGCT 6 cut(s) 182, 314, 346, 661, 683, 957
Alw21I GWGCWC 2 cut(s) 265, 718
Alw26I GTCTC 1 cut(s) 383
AlwI GGATC 1 cut(s) 558
AlwNI CAGNNNCTG 1 cut(s) 508
Ama87I CYCGRG 1 cut(s) 723
AoxI GGCC 1 cut(s) 305
ApeKI GCWGC 2 cut(s) 256, 728
ApoI RAATTY 2 cut(s) 355, 541
Asp718I GGTACC 1 cut(s) 890
AspLEI GCGC 1 cut(s) 827
AspS9I GGNCC 3 cut(s) 109, 305, 951
AsuHPI GGTGA 2 cut(s) 429, 581
AvaI CYCGRG 1 cut(s) 723
AvaII GGWCC 2 cut(s) 109, 951
BanI GGYRCC 1 cut(s) 890
BbsI GAAGAC 1 cut(s) 705
Bbv12I GWGCWC 2 cut(s) 265, 718
BbvI GCAGC 2 cut(s) 268, 740
BccI CCATC 4 cut(s) 70, 99, 140, 903
BciT130I CCWGG 2 cut(s) 419, 888
BclI TGATCA 1 cut(s) 633
BcoDI GTCTC 1 cut(s) 383
BfaI CTAG 4 cut(s) 68, 207, 447, 845
BfmI CTRYAG 3 cut(s) 254, 323, 408
BisI GCNGC 2 cut(s) 257, 729
BlpI GCTNAGC 1 cut(s) 813
BlsI GCNGC 2 cut(s) 258, 730
Bme1390I CCNGG 2 cut(s) 419, 888
Bme18I GGWCC 2 cut(s) 109, 951
BmeRI GACNNNNNGTC 1 cut(s) 949
BmeT110I CYCGRG 1 cut(s) 723
BmgT120I GGNCC 3 cut(s) 109, 305, 951
BmiI GGNNCC 4 cut(s) 110, 519, 892, 952
BmrFI CCNGG 2 cut(s) 419, 888
BmsI GCATC 3 cut(s) 210, 676, 742
BpiI GAAGAC 1 cut(s) 705
Bpu1102I GCTNAGC 1 cut(s) 813
BpuEI CTTGAG 2 cut(s) 474, 730
BsaBI GATNNNNATC 2 cut(s) 195, 528
BsaI GGTCTC 1 cut(s) 383
BsaJI CCNNGG 2 cut(s) 393, 946
Bsc4I CCNNNNNNNGG 2 cut(s) 106, 508
Bse1I ACTGG 2 cut(s) 211, 870
Bse3DI GCAATG 2 cut(s) 159, 922
Bse8I GATNNNNATC 2 cut(s) 195, 528
BseBI CCWGG 2 cut(s) 419, 888
BseDI CCNNGG 2 cut(s) 393, 946
BseGI GGATG 2 cut(s) 427, 561
BseJI GATNNNNATC 2 cut(s) 195, 528
BseLI CCNNNNNNNGG 2 cut(s) 106, 508
BseMI GCAATG 2 cut(s) 159, 922
BseMII CTCAG 4 cut(s) 179, 282, 639, 731
BseNI ACTGG 2 cut(s) 211, 870
BseXI GCAGC 2 cut(s) 268, 740
BshFI GGCC 1 cut(s) 307
BshNI GGYRCC 1 cut(s) 890
BsiHKAI GWGCWC 2 cut(s) 265, 718
BsiHKCI CYCGRG 1 cut(s) 723
BslFI GGGAC 1 cut(s) 122
BslI CCNNNNNNNGG 2 cut(s) 106, 508
BsmAI GTCTC 1 cut(s) 383
BsmFI GGGAC 1 cut(s) 122
BsnI GGCC 1 cut(s) 307
Bso31I GGTCTC 1 cut(s) 383
BsoBI CYCGRG 1 cut(s) 723
Bsp1286I GDGCHC 2 cut(s) 265, 718
Bsp143I GATC 3 cut(s) 550, 571, 633
Bsp1720I GCTNAGC 1 cut(s) 813
BspACI CCGC 1 cut(s) 737
BspANI GGCC 1 cut(s) 307
BspCNI CTCAG 4 cut(s) 178, 283, 640, 730
BspLI GGNNCC 4 cut(s) 110, 519, 892, 952
BspMAI CTGCAG 3 cut(s) 258, 327, 412
BspPI GGATC 1 cut(s) 558
BspQI GCTCTTC 1 cut(s) 270
BspT107I GGYRCC 1 cut(s) 890
BspTNI GGTCTC 1 cut(s) 383
BsrDI GCAATG 2 cut(s) 159, 922
BsrI ACTGG 2 cut(s) 211, 870
BssECI CCNNGG 2 cut(s) 393, 946
BssMI GATC 3 cut(s) 550, 571, 633
BssT1I CCWWGG 2 cut(s) 393, 946
Bst2UI CCWGG 2 cut(s) 419, 888
Bst4CI ACNGT 3 cut(s) 32, 113, 238
Bst6I CTCTTC 3 cut(s) 101, 255, 270
BstC8I GCNNGC 4 cut(s) 261, 323, 681, 856
BstDEI CTNAG 6 cut(s) 165, 291, 648, 717, 743, 813
BstF5I GGATG 2 cut(s) 427, 561
BstHHI GCGC 1 cut(s) 827
BstKTI GATC 3 cut(s) 553, 574, 636
BstMAI GTCTC 1 cut(s) 383
BstMBI GATC 3 cut(s) 550, 571, 633
BstMWI GCNNNNNNNGC 4 cut(s) 207, 279, 734, 801
BstNI CCWGG 2 cut(s) 419, 888
BstNSI RCATGY 1 cut(s) 566
BstSCI CCNGG 2 cut(s) 417, 886
BstSFI CTRYAG 3 cut(s) 254, 323, 408
BstV1I GCAGC 2 cut(s) 268, 740
BstV2I GAAGAC 1 cut(s) 705
BsuRI GGCC 1 cut(s) 307
BtgZI GCGATG 1 cut(s) 159
BtsCI GGATG 2 cut(s) 427, 561
BtsI GCAGTG 1 cut(s) 280
BtsIMutI CAGTG 5 cut(s) 280, 629, 768, 806, 863
Cac8I GCNNGC 4 cut(s) 261, 323, 681, 856
CaiI CAGNNNCTG 1 cut(s) 508
CfoI GCGC 1 cut(s) 827
Cfr13I GGNCC 3 cut(s) 109, 305, 951
Csp6I GTAC 5 cut(s) 214, 288, 425, 891, 938
CviAII CATG 1 cut(s) 563
CviQI GTAC 5 cut(s) 214, 288, 425, 891, 938
DdeI CTNAG 6 cut(s) 165, 291, 648, 717, 743, 813
DpnI GATC 3 cut(s) 552, 573, 635
DpnII GATC 3 cut(s) 550, 571, 633
DraIII CACNNNGTG 1 cut(s) 644
DriI GACNNNNNGTC 1 cut(s) 949
Eam1104I CTCTTC 3 cut(s) 101, 255, 270
Eam1105I GACNNNNNGTC 1 cut(s) 949
EarI CTCTTC 3 cut(s) 101, 255, 270
Eco130I CCWWGG 2 cut(s) 393, 946
Eco31I GGTCTC 1 cut(s) 383
Eco47I GGWCC 2 cut(s) 109, 951
Eco57I CTGAAG 2 cut(s) 717, 782
Eco88I CYCGRG 1 cut(s) 723
EcoRII CCWGG 2 cut(s) 417, 886
EcoT14I CCWWGG 2 cut(s) 393, 946
EcoT22I ATGCAT 1 cut(s) 924
ErhI CCWWGG 2 cut(s) 393, 946
FaeI CATG 1 cut(s) 566
FalI AAGNNNNNCTT 6 cut(s) 41, 73, 168, 200, 358, 390
FaqI GGGAC 1 cut(s) 122
FatI CATG 1 cut(s) 562
FauNDI CATATG 1 cut(s) 284
FbaI TGATCA 1 cut(s) 633
FblI GTMKAC 1 cut(s) 240
Fnu4HI GCNGC 2 cut(s) 257, 729
FokI GGATG 2 cut(s) 434, 568
Fsp4HI GCNGC 2 cut(s) 257, 729
FspBI CTAG 4 cut(s) 68, 207, 447, 845
GlaI GCGC 1 cut(s) 826
GluI GCNGC 2 cut(s) 257, 729
HaeIII GGCC 1 cut(s) 307
HhaI GCGC 1 cut(s) 827
Hin1II CATG 1 cut(s) 566
Hin6I GCGC 1 cut(s) 825
HinP1I GCGC 1 cut(s) 825
HindIII AAGCTT 2 cut(s) 344, 659
HinfI GANTC 5 cut(s) 37, 451, 505, 529, 721
HphI GGTGA 2 cut(s) 429, 581
Hpy166II GTNNAC 1 cut(s) 241
Hpy188I TCNGA 5 cut(s) 555, 618, 657, 697, 720
Hpy188III TCNNGA 5 cut(s) 461, 479, 491, 521, 845
Hpy8I GTNNAC 1 cut(s) 241
HpyAV CCTTC 3 cut(s) 143, 806, 842
HpyCH4III ACNGT 3 cut(s) 32, 113, 238
HpyCH4V TGCA 8 cut(s) 225, 256, 325, 410, 538, 766, 922, 927
HpyF10VI GCNNNNNNNGC 4 cut(s) 207, 279, 734, 801
HpyF3I CTNAG 6 cut(s) 165, 291, 648, 717, 743, 813
Hsp92II CATG 1 cut(s) 566
HspAI GCGC 1 cut(s) 825
KpnI GGTACC 1 cut(s) 894
Ksp22I TGATCA 1 cut(s) 633
Kzo9I GATC 3 cut(s) 550, 571, 633
LguI GCTCTTC 1 cut(s) 270
LmnI GCTCC 1 cut(s) 676
Lsp1109I GCAGC 2 cut(s) 268, 740
LweI GCATC 3 cut(s) 210, 676, 742
MaeI CTAG 4 cut(s) 68, 207, 447, 845
MalI GATC 3 cut(s) 552, 573, 635
MboI GATC 3 cut(s) 550, 571, 633
MboII GAAGA 6 cut(s) 88, 112, 242, 257, 566, 710
MhlI GDGCHC 2 cut(s) 265, 718
MluCI AATT 4 cut(s) 332, 355, 541, 875
MlyI GAGTC 3 cut(s) 46, 499, 715
MnlI CCTC 4 cut(s) 45, 110, 134, 552
Mph1103I ATGCAT 1 cut(s) 924
MseI TTAA 2 cut(s) 330, 579
MslI CAYNNNNRTG 1 cut(s) 567
MspR9I CCNGG 2 cut(s) 419, 888
MvaI CCWGG 2 cut(s) 419, 888
MwoI GCNNNNNNNGC 4 cut(s) 207, 279, 734, 801
NdeI CATATG 1 cut(s) 284
NdeII GATC 3 cut(s) 550, 571, 633
NlaIII CATG 1 cut(s) 566
NlaIV GGNNCC 4 cut(s) 110, 519, 892, 952
NsiI ATGCAT 1 cut(s) 924
NspI RCATGY 1 cut(s) 566
PciSI GCTCTTC 1 cut(s) 270
PfeI GAWTC 2 cut(s) 451, 529
PflMI CCANNNNNTGG 1 cut(s) 508
PfoI TCCNGGA 1 cut(s) 417
PkrI GCNGC 2 cut(s) 258, 730
PleI GAGTC 3 cut(s) 45, 499, 715
PpsI GAGTC 3 cut(s) 45, 499, 715
PsiI TTATAA 1 cut(s) 402
Psp6I CCWGG 2 cut(s) 417, 886
PspGI CCWGG 2 cut(s) 417, 886
PspN4I GGNNCC 4 cut(s) 110, 519, 892, 952
PspPI GGNCC 3 cut(s) 109, 305, 951
PstI CTGCAG 3 cut(s) 258, 327, 412
PstNI CAGNNNCTG 1 cut(s) 508
RsaI GTAC 5 cut(s) 215, 289, 426, 892, 939
RsaNI GTAC 5 cut(s) 214, 288, 425, 891, 938
RseI CAYNNNNRTG 1 cut(s) 567
SapI GCTCTTC 1 cut(s) 270
SaqAI TTAA 2 cut(s) 330, 579
SatI GCNGC 2 cut(s) 257, 729
Sau3AI GATC 3 cut(s) 550, 571, 633
Sau96I GGNCC 3 cut(s) 109, 305, 951
SchI GAGTC 3 cut(s) 46, 499, 715
ScrFI CCNGG 2 cut(s) 419, 888
SduI GDGCHC 2 cut(s) 265, 718
SfaNI GCATC 3 cut(s) 210, 676, 742
SfcI CTRYAG 3 cut(s) 254, 323, 408
SinI GGWCC 2 cut(s) 109, 951
SmiMI CAYNNNNRTG 1 cut(s) 567
SmlI CTYRAG 2 cut(s) 489, 709
SmoI CTYRAG 2 cut(s) 489, 709
Sse9I AATT 4 cut(s) 332, 355, 541, 875
SsiI CCGC 1 cut(s) 737
SspI AATATT 1 cut(s) 583
SspMI CTAG 4 cut(s) 68, 207, 447, 845
StyD4I CCNGG 2 cut(s) 417, 886
StyI CCWWGG 2 cut(s) 393, 946
TaaI ACNGT 3 cut(s) 32, 113, 238
TaqI TCGA 1 cut(s) 898
TaqII GACCGA 1 cut(s) 717
TasI AATT 4 cut(s) 332, 355, 541, 875
TatI WGTACW 2 cut(s) 287, 937
TfiI GAWTC 2 cut(s) 451, 529
Tru1I TTAA 2 cut(s) 330, 579
Tru9I TTAA 2 cut(s) 330, 579
TscAI CASTG 5 cut(s) 280, 636, 768, 806, 870
TseI GCWGC 2 cut(s) 256, 728
TspDTI ATGAA 2 cut(s) 17, 871
TspRI CASTG 5 cut(s) 280, 636, 768, 806, 870
Van91I CCANNNNNTGG 1 cut(s) 508
VpaK11BI GGWCC 2 cut(s) 109, 951
XapI RAATTY 2 cut(s) 355, 541
XbaI TCTAGA 1 cut(s) 844
XceI RCATGY 1 cut(s) 566
XmiI GTMKAC 1 cut(s) 240
XspI CTAG 4 cut(s) 68, 207, 447, 845
Zsp2I ATGCAT 1 cut(s) 924
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.