FvH4_6g30880

4,5-DOPA dioxygenase extradiol-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0017339
Physical Location & Seq
Forward (+)
24048042 .. 24049575
1534 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g30880.t1

Sequence Viewer

Length: 924 bp
ATGACATTCTCAGTGCTCTCTGGTTATATATATAGAGGTTTAAAGCTCGTTCATTGTCCAACCCAATCGAGCTTTATTGACTATAAATCTTTATTTAATTCCAAGAAGATGGGTAAGGAAGTGTTTGATACATTCTTCTTGATTCACGGAGCACCGCTGGTTCTGATCGACGACACTCTACCCGCCAGAACATTCTTGAAGTCATGGAGGGAGAAGATCTTACCGGAGAAACCCAAATATATTCTCGTGGTTTCCGGTCACTGGGAGACCAACGAACCCTCCATCAACGTCGTCGACCGCCATGAAACCATCCATGATTTCGAAGACTTCCCGCCCGAGATGTACGAGCTCCAGTACCCAGCTCCGGGATCCAAAGAACTGGCCACCATGGTGAACGACGTTCTGGTGGCTGCCGGGTACAAGCCTCTCCACGTAGACACGAAGCGAGGGCTCGATCACGGAGCCTGGGTGCCACTCATGCAAATGTACCCGGAAGCCGACGTGCCGGTGTGCCAGCTCTCGATCCAGTCCCACGAGAACGGAACCTATCACTTCAACTTGGGCAAGGCCCTGGCTCCTCTCAGGGAGCAAGGAGTCCTCATCGTCGGCACCGGAAGTGTCACGCACAACATGGAGTCCCTCATCGTCAACAACGTCGGCGGCACTGTGGCCCCGTGGGCTCTCGAGTTCGACAACTGGGTCAAGGACGCGCTGCTCAACGGAAGGTACGATGAGGTGAACGAGGCTCTGGAGAAACATCCACACGCCAAAATGGCGCATCCGGACGGACCGGTGCATTTCTATCCTCTGCTGGCGGCCATCGGGGCTGCCGGAGGGGAAAAGGCCAAGGCGGAGCTCATTCATCATAGCTTTAGCCGGCATACTATTTCCTATGCCAACTACCGGTTTAGGTCAACTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00965 map00965
Pfam Domains
Protein Families
LigB

Protein Analysis

308

Amino Acids

34.49

Weight (kDa)

6.09

Isoelectric Point (pI)

30.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LigB PF02900 44 - 303 8.7e-49 Catalytic LigB subunit of aromatic ring-opening dioxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017339)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g30880
malus_domestica MD05G1186100.v1.1
rosa_chinensis RchiOBHm_Chr2g0137351
rosa_laevigata RLG00000019652
rosa_multiflora Rmu_sc0003208.1_g000024
rosa_roxburghii Rroxscaffold_2G00105650 Rroxscaffold_2G00107130
rosa_rugosa Rorug02G0341300
rosa_samantha Rh2AG389800 Rh2BG397300 Rh2CG377300 Rh2DG412500
rosa_wichuraiana Rw2G031930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 698
AccB1I GGYRCC 2 cut(s) 469, 608
AccI GTMKAC 2 cut(s) 294, 435
AccII CGCG 1 cut(s) 710
AccIII TCCGGA 1 cut(s) 781
AciI CCGC 7 cut(s) 155, 183, 298, 332, 660, 815, 851
AclWI GGATC 3 cut(s) 363, 376, 517
AcoI YGGCCR 2 cut(s) 381, 816
AfaI GTAC 5 cut(s) 344, 356, 419, 488, 728
AfiI CCNNNNNNNGG 4 cut(s) 261, 364, 365, 903
AgeI ACCGGT 2 cut(s) 790, 903
AgsI TTSAA 2 cut(s) 199, 556
AjiI CACGTC 1 cut(s) 502
AjnI CCWGG 2 cut(s) 464, 570
AleI CACNNNNGTG 1 cut(s) 389
AluBI AGCT 7 cut(s) 46, 72, 349, 362, 517, 856, 870
AluI AGCT 7 cut(s) 46, 72, 349, 362, 517, 856, 870
Alw21I GWGCWC 4 cut(s) 18, 154, 351, 858
Alw26I GTCTC 1 cut(s) 260
AlwI GGATC 3 cut(s) 363, 376, 517
Ama87I CYCGRG 2 cut(s) 335, 683
Aor13HI TCCGGA 1 cut(s) 781
AoxI GGCC 5 cut(s) 381, 567, 669, 816, 843
ApeKI GCWGC 3 cut(s) 410, 712, 827
AsiGI ACCGGT 2 cut(s) 790, 903
AspLEI GCGC 2 cut(s) 712, 778
AspS9I GGNCC 3 cut(s) 568, 670, 788
AsuC2I CCSGG 3 cut(s) 366, 415, 491
AsuHPI GGTGA 2 cut(s) 403, 748
AsuII TTCGAA 1 cut(s) 321
AvaI CYCGRG 2 cut(s) 335, 683
AvaII GGWCC 1 cut(s) 788
BalI TGGCCA 1 cut(s) 383
BamHI GGATCC 1 cut(s) 368
BanI GGYRCC 2 cut(s) 469, 608
BanII GRGCYC 4 cut(s) 351, 453, 682, 858
BauI CACGAG 2 cut(s) 245, 533
BbsI GAAGAC 1 cut(s) 330
Bbv12I GWGCWC 4 cut(s) 18, 154, 351, 858
BbvI GCAGC 3 cut(s) 397, 699, 814
BccI CCATC 4 cut(s) 103, 290, 317, 827
BciT130I CCWGG 2 cut(s) 466, 572
BcnI CCSGG 3 cut(s) 366, 415, 491
BcoDI GTCTC 1 cut(s) 260
BglI GCCNNNNNGGC 3 cut(s) 677, 773, 824
BglII AGATCT 1 cut(s) 216
BisI GCNGC 5 cut(s) 411, 661, 713, 816, 828
BlsI GCNGC 5 cut(s) 412, 662, 714, 817, 829
Bme1390I CCNGG 5 cut(s) 366, 415, 466, 491, 572
Bme18I GGWCC 1 cut(s) 788
BmeT110I CYCGRG 2 cut(s) 335, 683
BmgBI CACGTC 1 cut(s) 502
BmgT120I GGNCC 3 cut(s) 568, 670, 788
BmiI GGNNCC 7 cut(s) 370, 463, 471, 544, 576, 610, 672
BmrFI CCNGG 5 cut(s) 366, 415, 466, 491, 572
BmrI ACTGGG 2 cut(s) 271, 706
BmsI GCATC 1 cut(s) 787
BmuI ACTGGG 2 cut(s) 271, 706
BpiI GAAGAC 1 cut(s) 330
BpmI CTGGAG 2 cut(s) 335, 770
Bpu14I TTCGAA 1 cut(s) 321
BpuMI CCSGG 3 cut(s) 366, 415, 491
BsaAI YACGTR 1 cut(s) 433
BsaI GGTCTC 1 cut(s) 260
BsaJI CCNNGG 5 cut(s) 387, 465, 570, 674, 846
BsaWI WCCGGW 6 cut(s) 223, 254, 611, 781, 790, 903
BsaXI ACNNNNNCTCC 4 cut(s) 263, 293, 453, 483
Bsc4I CCNNNNNNNGG 4 cut(s) 261, 364, 365, 903
Bse118I RCCGGY 4 cut(s) 505, 790, 876, 903
Bse1I ACTGG 5 cut(s) 266, 352, 384, 526, 701
BseAI TCCGGA 1 cut(s) 781
BseBI CCWGG 2 cut(s) 466, 572
BseDI CCNNGG 5 cut(s) 387, 465, 570, 674, 846
BseGI GGATG 3 cut(s) 309, 757, 778
BseLI CCNNNNNNNGG 4 cut(s) 261, 364, 365, 903
BseMII CTCAG 3 cut(s) 24, 595, 909
BseNI ACTGG 5 cut(s) 266, 352, 384, 526, 701
BseRI GAGGAG 1 cut(s) 567
BseXI GCAGC 3 cut(s) 397, 699, 814
BseYI CCCAGC 1 cut(s) 358
Bsh1236I CGCG 1 cut(s) 710
Bsh1285I CGRYCG 1 cut(s) 298
BshFI GGCC 5 cut(s) 383, 569, 671, 818, 845
BshNI GGYRCC 2 cut(s) 469, 608
BshTI ACCGGT 2 cut(s) 790, 903
BsiEI CGRYCG 1 cut(s) 298
BsiHKAI GWGCWC 4 cut(s) 18, 154, 351, 858
BsiHKCI CYCGRG 2 cut(s) 335, 683
BslFI GGGAC 2 cut(s) 514, 622
BslI CCNNNNNNNGG 4 cut(s) 261, 364, 365, 903
BsmAI GTCTC 1 cut(s) 260
BsmFI GGGAC 2 cut(s) 514, 622
BsnI GGCC 5 cut(s) 383, 569, 671, 818, 845
Bso31I GGTCTC 1 cut(s) 260
BsoBI CYCGRG 2 cut(s) 335, 683
Bsp119I TTCGAA 1 cut(s) 321
Bsp1286I GDGCHC 6 cut(s) 18, 154, 351, 453, 682, 858
Bsp13I TCCGGA 1 cut(s) 781
Bsp143I GATC 5 cut(s) 165, 216, 368, 454, 522
Bsp19I CCATGG 1 cut(s) 387
BspACI CCGC 7 cut(s) 155, 183, 298, 332, 660, 815, 851
BspANI GGCC 5 cut(s) 383, 569, 671, 818, 845
BspCNI CTCAG 3 cut(s) 23, 594, 910
BspEI TCCGGA 1 cut(s) 781
BspFNI CGCG 1 cut(s) 710
BspLI GGNNCC 7 cut(s) 370, 463, 471, 544, 576, 610, 672
BspPI GGATC 3 cut(s) 363, 376, 517
BspT104I TTCGAA 1 cut(s) 321
BspT107I GGYRCC 2 cut(s) 469, 608
BspTNI GGTCTC 1 cut(s) 260
BsrFI RCCGGY 4 cut(s) 505, 790, 876, 903
BsrI ACTGG 5 cut(s) 266, 352, 384, 526, 701
BssAI RCCGGY 4 cut(s) 505, 790, 876, 903
BssECI CCNNGG 5 cut(s) 387, 465, 570, 674, 846
BssMI GATC 5 cut(s) 165, 216, 368, 454, 522
BssSI CACGAG 2 cut(s) 245, 533
BssT1I CCWWGG 2 cut(s) 387, 846
Bst2BI CACGAG 2 cut(s) 245, 533
Bst2UI CCWGG 2 cut(s) 466, 572
Bst4CI ACNGT 1 cut(s) 667
BstBAI YACGTR 1 cut(s) 433
BstBI TTCGAA 1 cut(s) 321
BstC8I GCNNGC 3 cut(s) 515, 813, 878
BstDEI CTNAG 3 cut(s) 10, 581, 918
BstDSI CCRYGG 2 cut(s) 387, 674
BstF5I GGATG 3 cut(s) 309, 757, 778
BstFNI CGCG 1 cut(s) 710
BstHHI GCGC 2 cut(s) 712, 778
BstKTI GATC 5 cut(s) 168, 219, 371, 457, 525
BstMAI GTCTC 1 cut(s) 260
BstMBI GATC 5 cut(s) 165, 216, 368, 454, 522
BstMCI CGRYCG 1 cut(s) 298
BstMWI GCNNNNNNNGC 4 cut(s) 478, 677, 773, 824
BstNI CCWGG 2 cut(s) 466, 572
BstSCI CCNGG 5 cut(s) 364, 413, 464, 489, 570
BstUI CGCG 1 cut(s) 710
BstV1I GCAGC 3 cut(s) 397, 699, 814
BstV2I GAAGAC 1 cut(s) 330
BstX2I RGATCY 2 cut(s) 216, 368
BstXI CCANNNNNNTGG 2 cut(s) 109, 379
BstYI RGATCY 2 cut(s) 216, 368
BsuRI GGCC 5 cut(s) 383, 569, 671, 818, 845
BtgI CCRYGG 2 cut(s) 387, 674
BtrI CACGTC 1 cut(s) 502
BtsCI GGATG 3 cut(s) 309, 757, 778
BtsIMutI CAGTG 3 cut(s) 18, 259, 663
Cac8I GCNNGC 3 cut(s) 515, 813, 878
CfoI GCGC 2 cut(s) 712, 778
Cfr10I RCCGGY 4 cut(s) 505, 790, 876, 903
Cfr13I GGNCC 3 cut(s) 568, 670, 788
CpoI CGGWCCG 1 cut(s) 788
CseI GACGC 1 cut(s) 716
Csp6I GTAC 5 cut(s) 343, 355, 418, 487, 727
CspAI ACCGGT 2 cut(s) 790, 903
CspI CGGWCCG 1 cut(s) 788
CviAII CATG 6 cut(s) 204, 302, 314, 388, 478, 631
CviQI GTAC 5 cut(s) 343, 355, 418, 487, 727
DdeI CTNAG 3 cut(s) 10, 581, 918
DpnI GATC 5 cut(s) 167, 218, 370, 456, 524
DpnII GATC 5 cut(s) 165, 216, 368, 454, 522
DraI TTTAAA 1 cut(s) 42
DrdI GACNNNNNNGTC 1 cut(s) 698
DseDI GACNNNNNNGTC 1 cut(s) 698
EaeI YGGCCR 2 cut(s) 381, 816
EciI GGCGGA 1 cut(s) 866
Ecl136II GAGCTC 2 cut(s) 349, 856
Eco130I CCWWGG 2 cut(s) 387, 846
Eco24I GRGCYC 4 cut(s) 351, 453, 682, 858
Eco31I GGTCTC 1 cut(s) 260
Eco47I GGWCC 1 cut(s) 788
Eco53kI GAGCTC 2 cut(s) 349, 856
Eco88I CYCGRG 2 cut(s) 335, 683
EcoICRI GAGCTC 2 cut(s) 349, 856
EcoO109I RGGNCCY 1 cut(s) 568
EcoRII CCWGG 2 cut(s) 464, 570
EcoT14I CCWWGG 2 cut(s) 387, 846
EcoT38I GRGCYC 4 cut(s) 351, 453, 682, 858
ErhI CCWWGG 2 cut(s) 387, 846
FaeI CATG 6 cut(s) 207, 305, 317, 391, 481, 634
FaqI GGGAC 2 cut(s) 514, 622
FatI CATG 6 cut(s) 203, 301, 313, 387, 477, 630
FauI CCCGC 2 cut(s) 190, 339
FblI GTMKAC 2 cut(s) 294, 435
Fnu4HI GCNGC 5 cut(s) 411, 661, 713, 816, 828
FokI GGATG 3 cut(s) 296, 744, 765
FriOI GRGCYC 4 cut(s) 351, 453, 682, 858
Fsp4HI GCNGC 5 cut(s) 411, 661, 713, 816, 828
GlaI GCGC 2 cut(s) 711, 777
GluI GCNGC 5 cut(s) 411, 661, 713, 816, 828
GsaI CCCAGC 1 cut(s) 362
GsuI CTGGAG 2 cut(s) 335, 770
HaeIII GGCC 5 cut(s) 383, 569, 671, 818, 845
HgaI GACGC 1 cut(s) 716
HhaI GCGC 2 cut(s) 712, 778
Hin1II CATG 6 cut(s) 207, 305, 317, 391, 481, 634
Hin6I GCGC 2 cut(s) 710, 776
HinP1I GCGC 2 cut(s) 710, 776
HincII GTYRAC 3 cut(s) 295, 649, 915
HindII GTYRAC 3 cut(s) 295, 649, 915
HinfI GANTC 3 cut(s) 142, 594, 635
HphI GGTGA 2 cut(s) 403, 748
Hpy166II GTNNAC 6 cut(s) 295, 394, 436, 649, 739, 915
Hpy188I TCNGA 1 cut(s) 165
Hpy188III TCNNGA 6 cut(s) 139, 196, 520, 683, 749, 782
Hpy8I GTNNAC 6 cut(s) 295, 394, 436, 649, 739, 915
Hpy99I CGWCG 7 cut(s) 173, 293, 296, 401, 503, 608, 659
HpyAV CCTTC 1 cut(s) 717
HpyCH4III ACNGT 1 cut(s) 667
HpyCH4IV ACGT 5 cut(s) 288, 399, 432, 501, 654
HpyCH4V TGCA 2 cut(s) 481, 796
HpyF10VI GCNNNNNNNGC 4 cut(s) 478, 677, 773, 824
HpyF3I CTNAG 3 cut(s) 10, 581, 918
HpySE526I ACGT 5 cut(s) 288, 399, 432, 501, 654
Hsp92II CATG 6 cut(s) 207, 305, 317, 391, 481, 634
HspAI GCGC 2 cut(s) 710, 776
Kpn2I TCCGGA 1 cut(s) 781
KroI GCCGGC 1 cut(s) 876
KroNI GCCGGC 1 cut(s) 878
Kzo9I GATC 5 cut(s) 165, 216, 368, 454, 522
LmnI GCTCC 7 cut(s) 149, 354, 367, 461, 580, 586, 853
Lsp1109I GCAGC 3 cut(s) 397, 699, 814
LweI GCATC 1 cut(s) 787
MaeII ACGT 5 cut(s) 288, 399, 432, 501, 654
MaeIII GTNAC 2 cut(s) 257, 619
MalI GATC 5 cut(s) 167, 218, 370, 456, 524
MboI GATC 5 cut(s) 165, 216, 368, 454, 522
MboII GAAGA 4 cut(s) 118, 127, 226, 335
MflI RGATCY 2 cut(s) 216, 368
MhlI GDGCHC 6 cut(s) 18, 154, 351, 453, 682, 858
MlsI TGGCCA 1 cut(s) 383
MluCI AATT 1 cut(s) 97
MluNI TGGCCA 1 cut(s) 383
MlyI GAGTC 2 cut(s) 603, 644
MmeI TCCRAC 1 cut(s) 83
Mox20I TGGCCA 1 cut(s) 383
MroI TCCGGA 1 cut(s) 781
MroNI GCCGGC 1 cut(s) 876
MscI TGGCCA 1 cut(s) 383
MseI TTAA 2 cut(s) 41, 96
MslI CAYNNNNRTG 2 cut(s) 389, 482
Msp20I TGGCCA 1 cut(s) 383
MspA1I CMGCKG 1 cut(s) 157
MspR9I CCNGG 5 cut(s) 366, 415, 466, 491, 572
MvaI CCWGG 2 cut(s) 466, 572
MvnI CGCG 1 cut(s) 710
MwoI GCNNNNNNNGC 4 cut(s) 478, 677, 773, 824
NaeI GCCGGC 1 cut(s) 878
NciI CCSGG 3 cut(s) 366, 415, 491
NcoI CCATGG 1 cut(s) 387
NdeII GATC 5 cut(s) 165, 216, 368, 454, 522
NgoMIV GCCGGC 1 cut(s) 876
NlaIII CATG 6 cut(s) 207, 305, 317, 391, 481, 634
NlaIV GGNNCC 7 cut(s) 370, 463, 471, 544, 576, 610, 672
NmuCI GTSAC 2 cut(s) 257, 619
NspV TTCGAA 1 cut(s) 321
OliI CACNNNNGTG 1 cut(s) 389
PaeR7I CTCGAG 1 cut(s) 683
PcsI WCGNNNNNNNCGW 2 cut(s) 651, 726
PdiI GCCGGC 1 cut(s) 878
PfeI GAWTC 1 cut(s) 142
PfoI TCCNGGA 1 cut(s) 364
PinAI ACCGGT 2 cut(s) 790, 903
PkrI GCNGC 5 cut(s) 412, 662, 714, 817, 829
PleI GAGTC 2 cut(s) 602, 643
PpsI GAGTC 2 cut(s) 602, 643
Ppu21I YACGTR 1 cut(s) 433
Psp124BI GAGCTC 2 cut(s) 351, 858
Psp6I CCWGG 2 cut(s) 464, 570
PspFI CCCAGC 1 cut(s) 358
PspGI CCWGG 2 cut(s) 464, 570
PspN4I GGNNCC 7 cut(s) 370, 463, 471, 544, 576, 610, 672
PspPI GGNCC 3 cut(s) 568, 670, 788
PsuI RGATCY 2 cut(s) 216, 368
RsaI GTAC 5 cut(s) 344, 356, 419, 488, 728
RsaNI GTAC 5 cut(s) 343, 355, 418, 487, 727
RseI CAYNNNNRTG 2 cut(s) 389, 482
Rsr2I CGGWCCG 1 cut(s) 788
RsrII CGGWCCG 1 cut(s) 788
SacI GAGCTC 2 cut(s) 351, 858
SalI GTCGAC 1 cut(s) 293
SaqAI TTAA 2 cut(s) 41, 96
SatI GCNGC 5 cut(s) 411, 661, 713, 816, 828
Sau3AI GATC 5 cut(s) 165, 216, 368, 454, 522
Sau96I GGNCC 3 cut(s) 568, 670, 788
SchI GAGTC 2 cut(s) 603, 644
ScrFI CCNGG 5 cut(s) 366, 415, 466, 491, 572
SduI GDGCHC 6 cut(s) 18, 154, 351, 453, 682, 858
SfaNI GCATC 1 cut(s) 787
Sfr274I CTCGAG 1 cut(s) 683
SfuI TTCGAA 1 cut(s) 321
SinI GGWCC 1 cut(s) 788
SlaI CTCGAG 1 cut(s) 683
SmiMI CAYNNNNRTG 2 cut(s) 389, 482
SmlI CTYRAG 1 cut(s) 683
SmoI CTYRAG 1 cut(s) 683
Sse9I AATT 1 cut(s) 97
SsiI CCGC 7 cut(s) 155, 183, 298, 332, 660, 815, 851
SstI GAGCTC 2 cut(s) 351, 858
StyD4I CCNGG 5 cut(s) 364, 413, 464, 489, 570
StyI CCWWGG 2 cut(s) 387, 846
TaaI ACNGT 1 cut(s) 667
TaiI ACGT 5 cut(s) 291, 402, 435, 504, 657
TaqI TCGA 8 cut(s) 68, 168, 294, 321, 453, 521, 684, 690
TasI AATT 1 cut(s) 97
TauI GCSGC 2 cut(s) 663, 818
TfiI GAWTC 1 cut(s) 142
Tru1I TTAA 2 cut(s) 41, 96
Tru9I TTAA 2 cut(s) 41, 96
TscAI CASTG 3 cut(s) 18, 266, 670
TseFI GTSAC 2 cut(s) 257, 619
TseI GCWGC 3 cut(s) 410, 712, 827
Tsp45I GTSAC 2 cut(s) 257, 619
TspDTI ATGAA 3 cut(s) 41, 318, 851
TspGWI ACGGA 5 cut(s) 162, 474, 555, 735, 801
TspRI CASTG 3 cut(s) 18, 266, 670
VpaK11BI GGWCC 1 cut(s) 788
XhoI CTCGAG 1 cut(s) 683
XmiI GTMKAC 2 cut(s) 294, 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.