Rw2G031930

4,5-DOPA dioxygenase extradiol-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0017339
Physical Location & Seq
Forward (+)
52513095 .. 52514113
1019 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G031930.1

Sequence Viewer

Length: 768 bp
ATGGGTAAGGAAGTGTTTGATACATTCTTTTTGATTCACGGAGCACCGCTGGTGCTGATCGACGACACATTACCCGCCAGAACTTTCTTGAAGTCGTGGAGGGAGAAGATCTTGCCGGAGAAGCCCAAATATGTCCTCGTGATTTCGGGTCACTGGGAGACCAACGAACCCTCCATCAATGTCGTCGACCGCCATGAAACAATCCACGATTTCGAAGACTTCCCACCCGAGATGTACGAGCTCCAGTACCCAGCTCCGGGATCTCAAGAACTGGCCACCAAGGTGAACGACGTCCTAGTAGCCGCCGGGTACAAGCCTCTCCACGTGGACACAAAGCGAGGCCTCGACCACGGAGCCTGGCTCTCCATCCAGTCCCACGAGAACGGAACCTATCACTTCAACTTGGGCAAGGCCCTGGCTCCTCTCAGGGAGCAAGGAGTCCTCATCATCGGCACCGGAAGCGTCACCCACAACATGGAGTCCCTCATCATCAACAACGTCGGCGGCACCGTGGCCGACTGGGCTCTCGAGTTCGACGACTATGTCAAGGACGCGCTGCTCAACGGAAGGTACGATGAGGTGAACCAGGCTTTGGAGAAACATCCGCACGCCAAGATGGCTCACCCGGATGGACCAGTACATTTCTATCCCCTACTGGCAGCCATCGGGGCCGCCGGAGGGGAAAAAGCCAAGGCGGAGCTCATTCATCATAGCTTTAGCCGGCATACTATTTCCTATGCCAACTACCGGTTTAAGTCAGCTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00965 map00965
Pfam Domains
Protein Families
LigB

Protein Analysis

255

Amino Acids

28.51

Weight (kDa)

5.78

Isoelectric Point (pI)

27.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LigB PF02900 8 - 251 9.9e-33 Catalytic LigB subunit of aromatic ring-opening dioxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017339)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g30880
malus_domestica MD05G1186100.v1.1
rosa_chinensis RchiOBHm_Chr2g0137351
rosa_laevigata RLG00000019652
rosa_multiflora Rmu_sc0003208.1_g000024
rosa_roxburghii Rroxscaffold_2G00105650 Rroxscaffold_2G00107130
rosa_rugosa Rorug02G0341300
rosa_samantha Rh2AG389800 Rh2BG397300 Rh2CG377300 Rh2DG412500
rosa_wichuraiana Rw2G031930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 542
AatII GACGTC 1 cut(s) 294
AccB1I GGYRCC 2 cut(s) 452, 506
AccB7I CCANNNNNTGG 2 cut(s) 475, 592
AccI GTMKAC 1 cut(s) 186
AccII CGCG 1 cut(s) 554
AciI CCGC 8 cut(s) 47, 75, 190, 303, 504, 605, 672, 695
AclWI GGATC 1 cut(s) 268
AcoI YGGCCR 2 cut(s) 273, 513
AcvI CACGTG 1 cut(s) 325
AcyI GRCGYC 1 cut(s) 291
AfaI GTAC 5 cut(s) 236, 248, 311, 572, 639
AfiI CCNNNNNNNGG 6 cut(s) 256, 257, 475, 592, 678, 747
AgeI ACCGGT 1 cut(s) 747
AgsI TTSAA 2 cut(s) 91, 400
AjnI CCWGG 3 cut(s) 356, 414, 585
AjuI GAANNNNNNNTTGG 2 cut(s) 575, 607
AleI CACNNNNGTG 1 cut(s) 281
AluBI AGCT 5 cut(s) 241, 254, 700, 714, 761
AluI AGCT 5 cut(s) 241, 254, 700, 714, 761
Alw21I GWGCWC 3 cut(s) 46, 243, 702
Alw26I GTCTC 1 cut(s) 152
AlwI GGATC 1 cut(s) 268
Ama87I CYCGRG 2 cut(s) 227, 527
AoxI GGCC 5 cut(s) 273, 340, 411, 513, 669
ApeKI GCWGC 2 cut(s) 556, 659
AsiGI ACCGGT 1 cut(s) 747
AspLEI GCGC 1 cut(s) 556
AspS9I GGNCC 3 cut(s) 412, 632, 669
AsuC2I CCSGG 3 cut(s) 258, 307, 626
AsuHPI GGTGA 4 cut(s) 295, 457, 592, 614
AsuII TTCGAA 1 cut(s) 213
AvaI CYCGRG 2 cut(s) 227, 527
AvaII GGWCC 1 cut(s) 632
BalI TGGCCA 1 cut(s) 275
BanI GGYRCC 2 cut(s) 452, 506
BanII GRGCYC 3 cut(s) 243, 526, 702
BauI CACGAG 2 cut(s) 137, 377
BbrPI CACGTG 1 cut(s) 325
BbsI GAAGAC 1 cut(s) 222
Bbv12I GWGCWC 3 cut(s) 46, 243, 702
BbvI GCAGC 2 cut(s) 543, 671
BccI CCATC 5 cut(s) 182, 374, 610, 623, 671
BciT130I CCWGG 3 cut(s) 358, 416, 587
BcnI CCSGG 3 cut(s) 258, 307, 626
BcoDI GTCTC 1 cut(s) 152
BfaI CTAG 1 cut(s) 296
BglI GCCNNNNNGGC 3 cut(s) 521, 617, 668
BglII AGATCT 1 cut(s) 108
BisI GCNGC 5 cut(s) 303, 505, 557, 660, 672
BlsI GCNGC 5 cut(s) 304, 506, 558, 661, 673
Bme1390I CCNGG 6 cut(s) 258, 307, 358, 416, 587, 626
Bme18I GGWCC 1 cut(s) 632
BmeT110I CYCGRG 2 cut(s) 227, 527
BmgT120I GGNCC 3 cut(s) 412, 632, 669
BmiI GGNNCC 6 cut(s) 355, 388, 420, 454, 508, 670
BmrFI CCNGG 6 cut(s) 258, 307, 358, 416, 587, 626
BmrI ACTGGG 2 cut(s) 163, 529
BmuI ACTGGG 2 cut(s) 163, 529
BpiI GAAGAC 1 cut(s) 222
BplI GAGNNNNNCTC 2 cut(s) 345, 377
BpmI CTGGAG 1 cut(s) 227
Bpu14I TTCGAA 1 cut(s) 213
BpuEI CTTGAG 1 cut(s) 249
BpuMI CCSGG 3 cut(s) 258, 307, 626
BsaAI YACGTR 1 cut(s) 325
BsaHI GRCGYC 1 cut(s) 291
BsaI GGTCTC 1 cut(s) 152
BsaJI CCNNGG 5 cut(s) 279, 349, 414, 510, 690
BsaWI WCCGGW 2 cut(s) 455, 747
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bsc4I CCNNNNNNNGG 6 cut(s) 256, 257, 475, 592, 678, 747
Bse118I RCCGGY 2 cut(s) 720, 747
Bse1I ACTGG 7 cut(s) 158, 244, 276, 370, 524, 635, 660
BseBI CCWGG 3 cut(s) 358, 416, 587
BseDI CCNNGG 5 cut(s) 279, 349, 414, 510, 690
BseGI GGATG 3 cut(s) 366, 601, 634
BseLI CCNNNNNNNGG 6 cut(s) 256, 257, 475, 592, 678, 747
BseMII CTCAG 2 cut(s) 439, 753
BseNI ACTGG 7 cut(s) 158, 244, 276, 370, 524, 635, 660
BseRI GAGGAG 1 cut(s) 411
BseXI GCAGC 2 cut(s) 543, 671
BseYI CCCAGC 1 cut(s) 250
Bsh1236I CGCG 1 cut(s) 554
Bsh1285I CGRYCG 1 cut(s) 190
BshFI GGCC 5 cut(s) 275, 342, 413, 515, 671
BshNI GGYRCC 2 cut(s) 452, 506
BshTI ACCGGT 1 cut(s) 747
BsiEI CGRYCG 1 cut(s) 190
BsiHKAI GWGCWC 3 cut(s) 46, 243, 702
BsiHKCI CYCGRG 2 cut(s) 227, 527
BsiSI CCGG 8 cut(s) 116, 257, 306, 456, 626, 675, 721, 748
BslFI GGGAC 2 cut(s) 358, 466
BslI CCNNNNNNNGG 6 cut(s) 256, 257, 475, 592, 678, 747
BsmAI GTCTC 1 cut(s) 152
BsmFI GGGAC 2 cut(s) 358, 466
BsnI GGCC 5 cut(s) 275, 342, 413, 515, 671
Bso31I GGTCTC 1 cut(s) 152
BsoBI CYCGRG 2 cut(s) 227, 527
Bsp119I TTCGAA 1 cut(s) 213
Bsp1286I GDGCHC 4 cut(s) 46, 243, 526, 702
Bsp143I GATC 3 cut(s) 57, 108, 260
BspACI CCGC 8 cut(s) 47, 75, 190, 303, 504, 605, 672, 695
BspANI GGCC 5 cut(s) 275, 342, 413, 515, 671
BspCNI CTCAG 2 cut(s) 438, 754
BspFNI CGCG 1 cut(s) 554
BspLI GGNNCC 6 cut(s) 355, 388, 420, 454, 508, 670
BspPI GGATC 1 cut(s) 268
BspT104I TTCGAA 1 cut(s) 213
BspT107I GGYRCC 2 cut(s) 452, 506
BspTNI GGTCTC 1 cut(s) 152
BsrFI RCCGGY 2 cut(s) 720, 747
BsrI ACTGG 7 cut(s) 158, 244, 276, 370, 524, 635, 660
BssAI RCCGGY 2 cut(s) 720, 747
BssECI CCNNGG 5 cut(s) 279, 349, 414, 510, 690
BssMI GATC 3 cut(s) 57, 108, 260
BssNI GRCGYC 1 cut(s) 291
BssSI CACGAG 2 cut(s) 137, 377
BssT1I CCWWGG 2 cut(s) 279, 690
Bst2BI CACGAG 2 cut(s) 137, 377
Bst2UI CCWGG 3 cut(s) 358, 416, 587
Bst4CI ACNGT 1 cut(s) 511
BstACI GRCGYC 1 cut(s) 291
BstBAI YACGTR 1 cut(s) 325
BstBI TTCGAA 1 cut(s) 213
BstC8I GCNNGC 2 cut(s) 609, 722
BstDEI CTNAG 2 cut(s) 425, 762
BstDSI CCRYGG 2 cut(s) 349, 510
BstF5I GGATG 3 cut(s) 366, 601, 634
BstFNI CGCG 1 cut(s) 554
BstHHI GCGC 1 cut(s) 556
BstKTI GATC 3 cut(s) 60, 111, 263
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 3 cut(s) 57, 108, 260
BstMCI CGRYCG 1 cut(s) 190
BstMWI GCNNNNNNNGC 5 cut(s) 121, 459, 521, 617, 668
BstNI CCWGG 3 cut(s) 358, 416, 587
BstSCI CCNGG 6 cut(s) 256, 305, 356, 414, 585, 624
BstUI CGCG 1 cut(s) 554
BstV1I GCAGC 2 cut(s) 543, 671
BstV2I GAAGAC 1 cut(s) 222
BstX2I RGATCY 2 cut(s) 108, 260
BstYI RGATCY 2 cut(s) 108, 260
BsuRI GGCC 5 cut(s) 275, 342, 413, 515, 671
BtgI CCRYGG 2 cut(s) 349, 510
BtsCI GGATG 3 cut(s) 366, 601, 634
BtsIMutI CAGTG 1 cut(s) 151
Cac8I GCNNGC 2 cut(s) 609, 722
CfoI GCGC 1 cut(s) 556
Cfr10I RCCGGY 2 cut(s) 720, 747
Cfr13I GGNCC 3 cut(s) 412, 632, 669
CseI GACGC 2 cut(s) 451, 560
Csp6I GTAC 5 cut(s) 235, 247, 310, 571, 638
CspAI ACCGGT 1 cut(s) 747
CviAII CATG 2 cut(s) 194, 475
CviQI GTAC 5 cut(s) 235, 247, 310, 571, 638
DdeI CTNAG 2 cut(s) 425, 762
DpnI GATC 3 cut(s) 59, 110, 262
DpnII GATC 3 cut(s) 57, 108, 260
DrdI GACNNNNNNGTC 1 cut(s) 542
DseDI GACNNNNNNGTC 1 cut(s) 542
EaeI YGGCCR 2 cut(s) 273, 513
EciI GGCGGA 1 cut(s) 710
Ecl136II GAGCTC 2 cut(s) 241, 700
Eco130I CCWWGG 2 cut(s) 279, 690
Eco147I AGGCCT 1 cut(s) 342
Eco24I GRGCYC 3 cut(s) 243, 526, 702
Eco31I GGTCTC 1 cut(s) 152
Eco47I GGWCC 1 cut(s) 632
Eco53kI GAGCTC 2 cut(s) 241, 700
Eco72I CACGTG 1 cut(s) 325
Eco88I CYCGRG 2 cut(s) 227, 527
EcoICRI GAGCTC 2 cut(s) 241, 700
EcoO109I RGGNCCY 1 cut(s) 412
EcoRII CCWGG 3 cut(s) 356, 414, 585
EcoT14I CCWWGG 2 cut(s) 279, 690
EcoT38I GRGCYC 3 cut(s) 243, 526, 702
ErhI CCWWGG 2 cut(s) 279, 690
FaeI CATG 2 cut(s) 197, 478
FaiI YATR 7 cut(s) 132, 195, 476, 543, 711, 726, 738
FaqI GGGAC 2 cut(s) 358, 466
FatI CATG 2 cut(s) 193, 474
FauI CCCGC 1 cut(s) 82
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 5 cut(s) 303, 505, 557, 660, 672
FokI GGATG 3 cut(s) 353, 588, 641
FriOI GRGCYC 3 cut(s) 243, 526, 702
Fsp4HI GCNGC 5 cut(s) 303, 505, 557, 660, 672
FspBI CTAG 1 cut(s) 296
GlaI GCGC 1 cut(s) 555
GluI GCNGC 5 cut(s) 303, 505, 557, 660, 672
GsaI CCCAGC 1 cut(s) 254
GsuI CTGGAG 1 cut(s) 227
HaeIII GGCC 5 cut(s) 275, 342, 413, 515, 671
HapII CCGG 8 cut(s) 116, 257, 306, 456, 626, 675, 721, 748
HgaI GACGC 2 cut(s) 451, 560
HhaI GCGC 1 cut(s) 556
Hin1I GRCGYC 1 cut(s) 291
Hin1II CATG 2 cut(s) 197, 478
Hin6I GCGC 1 cut(s) 554
HinP1I GCGC 1 cut(s) 554
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HinfI GANTC 3 cut(s) 34, 438, 479
HpaII CCGG 8 cut(s) 116, 257, 306, 456, 626, 675, 721, 748
HphI GGTGA 4 cut(s) 295, 457, 592, 614
Hpy166II GTNNAC 4 cut(s) 187, 286, 328, 583
Hpy188III TCNNGA 4 cut(s) 88, 139, 266, 527
Hpy8I GTNNAC 4 cut(s) 187, 286, 328, 583
Hpy99I CGWCG 5 cut(s) 65, 188, 293, 503, 539
HpyAV CCTTC 1 cut(s) 561
HpyCH4III ACNGT 1 cut(s) 511
HpyCH4IV ACGT 3 cut(s) 291, 324, 498
HpyF10VI GCNNNNNNNGC 5 cut(s) 121, 459, 521, 617, 668
HpyF3I CTNAG 2 cut(s) 425, 762
HpySE526I ACGT 3 cut(s) 291, 324, 498
Hsp92I GRCGYC 1 cut(s) 291
Hsp92II CATG 2 cut(s) 197, 478
HspAI GCGC 1 cut(s) 554
KroI GCCGGC 1 cut(s) 720
KroNI GCCGGC 1 cut(s) 722
Kzo9I GATC 3 cut(s) 57, 108, 260
LmnI GCTCC 7 cut(s) 41, 246, 259, 353, 424, 430, 697
Lsp1109I GCAGC 2 cut(s) 543, 671
MaeI CTAG 1 cut(s) 296
MaeII ACGT 3 cut(s) 291, 324, 498
MaeIII GTNAC 2 cut(s) 149, 463
MalI GATC 3 cut(s) 59, 110, 262
MboI GATC 3 cut(s) 57, 108, 260
MboII GAAGA 2 cut(s) 118, 227
MflI RGATCY 2 cut(s) 108, 260
MhlI GDGCHC 4 cut(s) 46, 243, 526, 702
MlsI TGGCCA 1 cut(s) 275
MluNI TGGCCA 1 cut(s) 275
MlyI GAGTC 2 cut(s) 447, 488
Mox20I TGGCCA 1 cut(s) 275
MroNI GCCGGC 1 cut(s) 720
MscI TGGCCA 1 cut(s) 275
MseI TTAA 1 cut(s) 753
MslI CAYNNNNRTG 2 cut(s) 281, 627
Msp20I TGGCCA 1 cut(s) 275
MspA1I CMGCKG 2 cut(s) 49, 761
MspI CCGG 8 cut(s) 116, 257, 306, 456, 626, 675, 721, 748
MspR9I CCNGG 6 cut(s) 258, 307, 358, 416, 587, 626
MvaI CCWGG 3 cut(s) 358, 416, 587
MvnI CGCG 1 cut(s) 554
MwoI GCNNNNNNNGC 5 cut(s) 121, 459, 521, 617, 668
NaeI GCCGGC 1 cut(s) 722
NciI CCSGG 3 cut(s) 258, 307, 626
NdeII GATC 3 cut(s) 57, 108, 260
NgoMIV GCCGGC 1 cut(s) 720
NlaIII CATG 2 cut(s) 197, 478
NlaIV GGNNCC 6 cut(s) 355, 388, 420, 454, 508, 670
NmuCI GTSAC 2 cut(s) 149, 463
NspV TTCGAA 1 cut(s) 213
OliI CACNNNNGTG 1 cut(s) 281
PaeR7I CTCGAG 1 cut(s) 527
PceI AGGCCT 1 cut(s) 342
PcsI WCGNNNNNNNCGW 3 cut(s) 507, 534, 570
PdiI GCCGGC 1 cut(s) 722
PfeI GAWTC 1 cut(s) 34
PflFI GACNNNGTC 1 cut(s) 542
PflMI CCANNNNNTGG 2 cut(s) 475, 592
PfoI TCCNGGA 1 cut(s) 256
PinAI ACCGGT 1 cut(s) 747
PkrI GCNGC 5 cut(s) 304, 506, 558, 661, 673
PleI GAGTC 2 cut(s) 446, 487
PmaCI CACGTG 1 cut(s) 325
PmlI CACGTG 1 cut(s) 325
PpsI GAGTC 2 cut(s) 446, 487
Ppu21I YACGTR 1 cut(s) 325
Psp124BI GAGCTC 2 cut(s) 243, 702
Psp6I CCWGG 3 cut(s) 356, 414, 585
PspCI CACGTG 1 cut(s) 325
PspFI CCCAGC 1 cut(s) 250
PspGI CCWGG 3 cut(s) 356, 414, 585
PspN4I GGNNCC 6 cut(s) 355, 388, 420, 454, 508, 670
PspPI GGNCC 3 cut(s) 412, 632, 669
PsuI RGATCY 2 cut(s) 108, 260
PsyI GACNNNGTC 1 cut(s) 542
PvuII CAGCTG 1 cut(s) 761
RsaI GTAC 5 cut(s) 236, 248, 311, 572, 639
RsaNI GTAC 5 cut(s) 235, 247, 310, 571, 638
RseI CAYNNNNRTG 2 cut(s) 281, 627
SacI GAGCTC 2 cut(s) 243, 702
SalI GTCGAC 1 cut(s) 185
SaqAI TTAA 1 cut(s) 753
SatI GCNGC 5 cut(s) 303, 505, 557, 660, 672
Sau3AI GATC 3 cut(s) 57, 108, 260
Sau96I GGNCC 3 cut(s) 412, 632, 669
SchI GAGTC 2 cut(s) 447, 488
ScrFI CCNGG 6 cut(s) 258, 307, 358, 416, 587, 626
SduI GDGCHC 4 cut(s) 46, 243, 526, 702
Sfr274I CTCGAG 1 cut(s) 527
SfuI TTCGAA 1 cut(s) 213
SinI GGWCC 1 cut(s) 632
SlaI CTCGAG 1 cut(s) 527
SmiMI CAYNNNNRTG 2 cut(s) 281, 627
SmlI CTYRAG 2 cut(s) 264, 527
SmoI CTYRAG 2 cut(s) 264, 527
SseBI AGGCCT 1 cut(s) 342
SsiI CCGC 8 cut(s) 47, 75, 190, 303, 504, 605, 672, 695
SspMI CTAG 1 cut(s) 296
SstI GAGCTC 2 cut(s) 243, 702
StuI AGGCCT 1 cut(s) 342
StyD4I CCNGG 6 cut(s) 256, 305, 356, 414, 585, 624
StyI CCWWGG 2 cut(s) 279, 690
TaaI ACNGT 1 cut(s) 511
TaiI ACGT 3 cut(s) 294, 327, 501
TaqI TCGA 6 cut(s) 60, 186, 213, 345, 528, 534
TatI WGTACW 1 cut(s) 637
TauI GCSGC 3 cut(s) 305, 507, 674
TfiI GAWTC 1 cut(s) 34
Tru1I TTAA 1 cut(s) 753
Tru9I TTAA 1 cut(s) 753
TscAI CASTG 1 cut(s) 158
TseFI GTSAC 2 cut(s) 149, 463
TseI GCWGC 2 cut(s) 556, 659
Tsp45I GTSAC 2 cut(s) 149, 463
TspDTI ATGAA 2 cut(s) 210, 695
TspGWI ACGGA 4 cut(s) 54, 366, 399, 579
TspRI CASTG 1 cut(s) 158
Tth111I GACNNNGTC 1 cut(s) 542
Van91I CCANNNNNTGG 2 cut(s) 475, 592
VpaK11BI GGWCC 1 cut(s) 632
XhoI CTCGAG 1 cut(s) 527
XmiI GTMKAC 1 cut(s) 186
XspI CTAG 1 cut(s) 296
ZraI GACGTC 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.