Rh2DG412500

4,5-DOPA dioxygenase extradiol-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0017339
Physical Location & Seq
Forward (+)
59911248 .. 59912862
1615 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG412500.1

Sequence Viewer

Length: 816 bp
ATGGGTAAGGAAGTGTTTGATACATTCTTTTTGATTCACGGAGCACCGCTGGTGCTGATCGACGACACATTACCTGCCAGAACTTTCTTGAAGTCGTGGAGGGAGAAGATCTTGCCGGAGAAGCCCAAATATGTCCTCGTGATTTCGGGTCACTGGGAGACCAACGAACCCTCCATCAATGTCGTCGACCGCCATGAAACAATCCACGATTTCGAAGACTTCCCGCCCGAGATGTACGAGCTCCAGTACCCAGCTCCGGGATCTCAAGAACTGGCCACCAAGGTGAACGACGTCCTAGTGGCCGCCGGGTACAAGCCTCTCCACGTGGACACAAAGCGAGGCCTCGACCACGGAGCCTGGGTCCCACTCATGCAAATGTACCCCGAAGCCGACTTGCCGGTGTGCCAGCTCTCCATCCAGTCCCACGAGAACGGAACCTATCACTTCAACTTGGGCAAGGCCCTGGCTCCTCTCAGGGATCAAGGAGTTCTCATCATCGGCACCGGAAGCGTCACCCACAACATGGAGTCCCTCATCATCAACAACGTCGGCGGCACCGTGGCCGACTGGGCTCTCGAGTTCGACGACTATGTCAAGGACGCGCTGCTCAACGGAAGGTACGATGAGGTGAACCAGGTTTTGGAGAAACATCCGCACGCCAAGATGGCTCACCCGGATGGACCAGTGCATTTCTATCCCCTACTGGCGGCCATCGGGGCCGCCGGAGGGGAAAAATCCAAGGCGGAGCTCATTCATCATAGCTTTAGCCGGCATACTATTTCCTATGCCAACTACCGGTTTAAGTCAGCTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00965 map00965
Pfam Domains
Protein Families
LigB

Protein Analysis

271

Amino Acids

30.36

Weight (kDa)

5.61

Isoelectric Point (pI)

27.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LigB PF02900 8 - 267 6.6e-47 Catalytic LigB subunit of aromatic ring-opening dioxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017339)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g30880
malus_domestica MD05G1186100.v1.1
rosa_chinensis RchiOBHm_Chr2g0137351
rosa_laevigata RLG00000019652
rosa_multiflora Rmu_sc0003208.1_g000024
rosa_roxburghii Rroxscaffold_2G00105650 Rroxscaffold_2G00107130
rosa_rugosa Rorug02G0341300
rosa_samantha Rh2AG389800 Rh2BG397300 Rh2CG377300 Rh2DG412500
rosa_wichuraiana Rw2G031930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 590
AatII GACGTC 1 cut(s) 294
Acc36I ACCTGC 1 cut(s) 82
AccB1I GGYRCC 2 cut(s) 500, 554
AccB7I CCANNNNNTGG 2 cut(s) 523, 640
AccI GTMKAC 1 cut(s) 186
AccII CGCG 1 cut(s) 602
AciI CCGC 9 cut(s) 47, 190, 224, 303, 552, 653, 707, 720, 743
AclWI GGATC 2 cut(s) 268, 486
AcoI YGGCCR 4 cut(s) 273, 300, 561, 708
AcvI CACGTG 1 cut(s) 325
AcyI GRCGYC 1 cut(s) 291
AfaI GTAC 5 cut(s) 236, 248, 311, 380, 620
AfiI CCNNNNNNNGG 7 cut(s) 256, 257, 523, 640, 706, 726, 795
AgeI ACCGGT 1 cut(s) 795
AgsI TTSAA 2 cut(s) 91, 448
AjnI CCWGG 3 cut(s) 356, 462, 633
AjuI GAANNNNNNNTTGG 2 cut(s) 623, 655
AleI CACNNNNGTG 1 cut(s) 281
AluBI AGCT 6 cut(s) 241, 254, 409, 748, 762, 809
AluI AGCT 6 cut(s) 241, 254, 409, 748, 762, 809
Alw21I GWGCWC 3 cut(s) 46, 243, 750
Alw26I GTCTC 1 cut(s) 152
AlwI GGATC 2 cut(s) 268, 486
Ama87I CYCGRG 2 cut(s) 227, 575
AoxI GGCC 7 cut(s) 273, 300, 340, 459, 561, 708, 717
ApeKI GCWGC 1 cut(s) 604
AsiGI ACCGGT 1 cut(s) 795
AspLEI GCGC 1 cut(s) 604
AspS9I GGNCC 4 cut(s) 361, 460, 680, 717
AsuC2I CCSGG 3 cut(s) 258, 307, 674
AsuHPI GGTGA 4 cut(s) 295, 505, 640, 662
AsuII TTCGAA 1 cut(s) 213
AvaI CYCGRG 2 cut(s) 227, 575
AvaII GGWCC 2 cut(s) 361, 680
BalI TGGCCA 1 cut(s) 275
BanI GGYRCC 2 cut(s) 500, 554
BanII GRGCYC 3 cut(s) 243, 574, 750
BauI CACGAG 2 cut(s) 137, 425
BbrPI CACGTG 1 cut(s) 325
BbsI GAAGAC 1 cut(s) 222
Bbv12I GWGCWC 3 cut(s) 46, 243, 750
BbvI GCAGC 1 cut(s) 591
BccI CCATC 5 cut(s) 182, 422, 658, 671, 719
BciT130I CCWGG 3 cut(s) 358, 464, 635
BcnI CCSGG 3 cut(s) 258, 307, 674
BcoDI GTCTC 1 cut(s) 152
BfaI CTAG 1 cut(s) 296
BfuAI ACCTGC 1 cut(s) 82
BglI GCCNNNNNGGC 3 cut(s) 569, 665, 716
BglII AGATCT 1 cut(s) 108
BisI GCNGC 5 cut(s) 303, 553, 605, 708, 720
BlsI GCNGC 5 cut(s) 304, 554, 606, 709, 721
Bme1390I CCNGG 6 cut(s) 258, 307, 358, 464, 635, 674
Bme18I GGWCC 2 cut(s) 361, 680
BmeT110I CYCGRG 2 cut(s) 227, 575
BmgT120I GGNCC 4 cut(s) 361, 460, 680, 717
BmiI GGNNCC 8 cut(s) 355, 362, 363, 436, 468, 502, 556, 718
BmrFI CCNGG 6 cut(s) 258, 307, 358, 464, 635, 674
BmrI ACTGGG 2 cut(s) 163, 577
BmuI ACTGGG 2 cut(s) 163, 577
BpiI GAAGAC 1 cut(s) 222
BpmI CTGGAG 1 cut(s) 227
Bpu14I TTCGAA 1 cut(s) 213
BpuEI CTTGAG 1 cut(s) 249
BpuMI CCSGG 3 cut(s) 258, 307, 674
BsaAI YACGTR 1 cut(s) 325
BsaHI GRCGYC 1 cut(s) 291
BsaI GGTCTC 1 cut(s) 152
BsaJI CCNNGG 6 cut(s) 279, 349, 357, 462, 558, 738
BsaWI WCCGGW 2 cut(s) 503, 795
BsaXI ACNNNNNCTCC 4 cut(s) 155, 185, 345, 375
Bsc4I CCNNNNNNNGG 7 cut(s) 256, 257, 523, 640, 706, 726, 795
Bse118I RCCGGY 3 cut(s) 397, 768, 795
Bse1I ACTGG 7 cut(s) 158, 244, 276, 418, 572, 683, 708
BseBI CCWGG 3 cut(s) 358, 464, 635
BseDI CCNNGG 6 cut(s) 279, 349, 357, 462, 558, 738
BseGI GGATG 3 cut(s) 414, 649, 682
BseLI CCNNNNNNNGG 7 cut(s) 256, 257, 523, 640, 706, 726, 795
BseMII CTCAG 2 cut(s) 487, 801
BseNI ACTGG 7 cut(s) 158, 244, 276, 418, 572, 683, 708
BseRI GAGGAG 1 cut(s) 459
BseXI GCAGC 1 cut(s) 591
BseYI CCCAGC 1 cut(s) 250
Bsh1236I CGCG 1 cut(s) 602
Bsh1285I CGRYCG 1 cut(s) 190
BshFI GGCC 7 cut(s) 275, 302, 342, 461, 563, 710, 719
BshNI GGYRCC 2 cut(s) 500, 554
BshTI ACCGGT 1 cut(s) 795
BsiEI CGRYCG 1 cut(s) 190
BsiHKAI GWGCWC 3 cut(s) 46, 243, 750
BsiHKCI CYCGRG 2 cut(s) 227, 575
BsiSI CCGG 9 cut(s) 116, 257, 306, 398, 504, 674, 723, 769, 796
BslFI GGGAC 3 cut(s) 347, 406, 514
BslI CCNNNNNNNGG 7 cut(s) 256, 257, 523, 640, 706, 726, 795
BsmAI GTCTC 1 cut(s) 152
BsmFI GGGAC 3 cut(s) 347, 406, 514
BsnI GGCC 7 cut(s) 275, 302, 342, 461, 563, 710, 719
Bso31I GGTCTC 1 cut(s) 152
BsoBI CYCGRG 2 cut(s) 227, 575
Bsp119I TTCGAA 1 cut(s) 213
Bsp1286I GDGCHC 4 cut(s) 46, 243, 574, 750
Bsp143I GATC 4 cut(s) 57, 108, 260, 478
BspACI CCGC 9 cut(s) 47, 190, 224, 303, 552, 653, 707, 720, 743
BspANI GGCC 7 cut(s) 275, 302, 342, 461, 563, 710, 719
BspCNI CTCAG 2 cut(s) 486, 802
BspFNI CGCG 1 cut(s) 602
BspLI GGNNCC 8 cut(s) 355, 362, 363, 436, 468, 502, 556, 718
BspMI ACCTGC 1 cut(s) 82
BspPI GGATC 2 cut(s) 268, 486
BspT104I TTCGAA 1 cut(s) 213
BspT107I GGYRCC 2 cut(s) 500, 554
BspTNI GGTCTC 1 cut(s) 152
BsrFI RCCGGY 3 cut(s) 397, 768, 795
BsrI ACTGG 7 cut(s) 158, 244, 276, 418, 572, 683, 708
BssAI RCCGGY 3 cut(s) 397, 768, 795
BssECI CCNNGG 6 cut(s) 279, 349, 357, 462, 558, 738
BssMI GATC 4 cut(s) 57, 108, 260, 478
BssNI GRCGYC 1 cut(s) 291
BssSI CACGAG 2 cut(s) 137, 425
BssT1I CCWWGG 2 cut(s) 279, 738
Bst2BI CACGAG 2 cut(s) 137, 425
Bst2UI CCWGG 3 cut(s) 358, 464, 635
Bst4CI ACNGT 1 cut(s) 559
BstACI GRCGYC 1 cut(s) 291
BstBAI YACGTR 1 cut(s) 325
BstBI TTCGAA 1 cut(s) 213
BstC8I GCNNGC 3 cut(s) 407, 657, 770
BstDEI CTNAG 2 cut(s) 473, 810
BstDSI CCRYGG 2 cut(s) 349, 558
BstF5I GGATG 3 cut(s) 414, 649, 682
BstFNI CGCG 1 cut(s) 602
BstHHI GCGC 1 cut(s) 604
BstKTI GATC 4 cut(s) 60, 111, 263, 481
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 4 cut(s) 57, 108, 260, 478
BstMCI CGRYCG 1 cut(s) 190
BstMWI GCNNNNNNNGC 5 cut(s) 121, 507, 569, 665, 716
BstNI CCWGG 3 cut(s) 358, 464, 635
BstSCI CCNGG 6 cut(s) 256, 305, 356, 462, 633, 672
BstUI CGCG 1 cut(s) 602
BstV1I GCAGC 1 cut(s) 591
BstV2I GAAGAC 1 cut(s) 222
BstX2I RGATCY 2 cut(s) 108, 260
BstYI RGATCY 2 cut(s) 108, 260
BsuRI GGCC 7 cut(s) 275, 302, 342, 461, 563, 710, 719
BtgI CCRYGG 2 cut(s) 349, 558
BtsCI GGATG 3 cut(s) 414, 649, 682
BtsIMutI CAGTG 2 cut(s) 151, 690
BveI ACCTGC 1 cut(s) 82
Cac8I GCNNGC 3 cut(s) 407, 657, 770
CfoI GCGC 1 cut(s) 604
Cfr10I RCCGGY 3 cut(s) 397, 768, 795
Cfr13I GGNCC 4 cut(s) 361, 460, 680, 717
CseI GACGC 2 cut(s) 499, 608
CsiI ACCWGGT 1 cut(s) 633
Csp6I GTAC 5 cut(s) 235, 247, 310, 379, 619
CspAI ACCGGT 1 cut(s) 795
CviAII CATG 3 cut(s) 194, 370, 523
CviQI GTAC 5 cut(s) 235, 247, 310, 379, 619
DdeI CTNAG 2 cut(s) 473, 810
DpnI GATC 4 cut(s) 59, 110, 262, 480
DpnII GATC 4 cut(s) 57, 108, 260, 478
DrdI GACNNNNNNGTC 1 cut(s) 590
DseDI GACNNNNNNGTC 1 cut(s) 590
EaeI YGGCCR 4 cut(s) 273, 300, 561, 708
EciI GGCGGA 1 cut(s) 758
Ecl136II GAGCTC 2 cut(s) 241, 748
Eco130I CCWWGG 2 cut(s) 279, 738
Eco147I AGGCCT 1 cut(s) 342
Eco24I GRGCYC 3 cut(s) 243, 574, 750
Eco31I GGTCTC 1 cut(s) 152
Eco47I GGWCC 2 cut(s) 361, 680
Eco53kI GAGCTC 2 cut(s) 241, 748
Eco72I CACGTG 1 cut(s) 325
Eco88I CYCGRG 2 cut(s) 227, 575
EcoICRI GAGCTC 2 cut(s) 241, 748
EcoO109I RGGNCCY 2 cut(s) 361, 460
EcoRII CCWGG 3 cut(s) 356, 462, 633
EcoT14I CCWWGG 2 cut(s) 279, 738
EcoT38I GRGCYC 3 cut(s) 243, 574, 750
ErhI CCWWGG 2 cut(s) 279, 738
FaeI CATG 3 cut(s) 197, 373, 526
FaiI YATR 8 cut(s) 132, 195, 371, 524, 591, 759, 774, 786
FaqI GGGAC 3 cut(s) 347, 406, 514
FatI CATG 3 cut(s) 193, 369, 522
FauI CCCGC 1 cut(s) 231
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 5 cut(s) 303, 553, 605, 708, 720
FokI GGATG 3 cut(s) 401, 636, 689
FriOI GRGCYC 3 cut(s) 243, 574, 750
Fsp4HI GCNGC 5 cut(s) 303, 553, 605, 708, 720
FspBI CTAG 1 cut(s) 296
GlaI GCGC 1 cut(s) 603
GluI GCNGC 5 cut(s) 303, 553, 605, 708, 720
GsaI CCCAGC 1 cut(s) 254
GsuI CTGGAG 1 cut(s) 227
HaeIII GGCC 7 cut(s) 275, 302, 342, 461, 563, 710, 719
HapII CCGG 9 cut(s) 116, 257, 306, 398, 504, 674, 723, 769, 796
HgaI GACGC 2 cut(s) 499, 608
HhaI GCGC 1 cut(s) 604
Hin1I GRCGYC 1 cut(s) 291
Hin1II CATG 3 cut(s) 197, 373, 526
Hin6I GCGC 1 cut(s) 602
HinP1I GCGC 1 cut(s) 602
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HinfI GANTC 2 cut(s) 34, 527
HpaII CCGG 9 cut(s) 116, 257, 306, 398, 504, 674, 723, 769, 796
HphI GGTGA 4 cut(s) 295, 505, 640, 662
Hpy166II GTNNAC 4 cut(s) 187, 286, 328, 631
Hpy188III TCNNGA 4 cut(s) 88, 139, 266, 575
Hpy8I GTNNAC 4 cut(s) 187, 286, 328, 631
Hpy99I CGWCG 5 cut(s) 65, 188, 293, 551, 587
HpyAV CCTTC 1 cut(s) 609
HpyCH4III ACNGT 1 cut(s) 559
HpyCH4IV ACGT 3 cut(s) 291, 324, 546
HpyCH4V TGCA 2 cut(s) 373, 688
HpyF10VI GCNNNNNNNGC 5 cut(s) 121, 507, 569, 665, 716
HpyF3I CTNAG 2 cut(s) 473, 810
HpySE526I ACGT 3 cut(s) 291, 324, 546
Hsp92I GRCGYC 1 cut(s) 291
Hsp92II CATG 3 cut(s) 197, 373, 526
HspAI GCGC 1 cut(s) 602
KflI GGGWCCC 1 cut(s) 361
KroI GCCGGC 1 cut(s) 768
KroNI GCCGGC 1 cut(s) 770
Kzo9I GATC 4 cut(s) 57, 108, 260, 478
LmnI GCTCC 6 cut(s) 41, 246, 259, 353, 472, 745
Lsp1109I GCAGC 1 cut(s) 591
MabI ACCWGGT 1 cut(s) 633
MaeI CTAG 1 cut(s) 296
MaeII ACGT 3 cut(s) 291, 324, 546
MaeIII GTNAC 2 cut(s) 149, 511
MalI GATC 4 cut(s) 59, 110, 262, 480
MboI GATC 4 cut(s) 57, 108, 260, 478
MboII GAAGA 2 cut(s) 118, 227
MflI RGATCY 2 cut(s) 108, 260
MhlI GDGCHC 4 cut(s) 46, 243, 574, 750
MlsI TGGCCA 1 cut(s) 275
MluNI TGGCCA 1 cut(s) 275
MlyI GAGTC 1 cut(s) 536
Mox20I TGGCCA 1 cut(s) 275
MroNI GCCGGC 1 cut(s) 768
MscI TGGCCA 1 cut(s) 275
MseI TTAA 1 cut(s) 801
MslI CAYNNNNRTG 3 cut(s) 281, 374, 675
Msp20I TGGCCA 1 cut(s) 275
MspA1I CMGCKG 2 cut(s) 49, 809
MspI CCGG 9 cut(s) 116, 257, 306, 398, 504, 674, 723, 769, 796
MspR9I CCNGG 6 cut(s) 258, 307, 358, 464, 635, 674
MvaI CCWGG 3 cut(s) 358, 464, 635
MvnI CGCG 1 cut(s) 602
MwoI GCNNNNNNNGC 5 cut(s) 121, 507, 569, 665, 716
NaeI GCCGGC 1 cut(s) 770
NciI CCSGG 3 cut(s) 258, 307, 674
NdeII GATC 4 cut(s) 57, 108, 260, 478
NgoMIV GCCGGC 1 cut(s) 768
NlaIII CATG 3 cut(s) 197, 373, 526
NlaIV GGNNCC 8 cut(s) 355, 362, 363, 436, 468, 502, 556, 718
NmuCI GTSAC 2 cut(s) 149, 511
NspV TTCGAA 1 cut(s) 213
OliI CACNNNNGTG 1 cut(s) 281
PaeR7I CTCGAG 1 cut(s) 575
PceI AGGCCT 1 cut(s) 342
PcsI WCGNNNNNNNCGW 3 cut(s) 555, 582, 618
PdiI GCCGGC 1 cut(s) 770
PfeI GAWTC 1 cut(s) 34
PflFI GACNNNGTC 1 cut(s) 590
PflMI CCANNNNNTGG 2 cut(s) 523, 640
PfoI TCCNGGA 1 cut(s) 256
PinAI ACCGGT 1 cut(s) 795
PkrI GCNGC 5 cut(s) 304, 554, 606, 709, 721
PleI GAGTC 1 cut(s) 535
PmaCI CACGTG 1 cut(s) 325
PmlI CACGTG 1 cut(s) 325
PpsI GAGTC 1 cut(s) 535
Ppu21I YACGTR 1 cut(s) 325
PpuMI RGGWCCY 1 cut(s) 361
Psp124BI GAGCTC 2 cut(s) 243, 750
Psp5II RGGWCCY 1 cut(s) 361
Psp6I CCWGG 3 cut(s) 356, 462, 633
PspCI CACGTG 1 cut(s) 325
PspFI CCCAGC 1 cut(s) 250
PspGI CCWGG 3 cut(s) 356, 462, 633
PspN4I GGNNCC 8 cut(s) 355, 362, 363, 436, 468, 502, 556, 718
PspPI GGNCC 4 cut(s) 361, 460, 680, 717
PspPPI RGGWCCY 1 cut(s) 361
PsuI RGATCY 2 cut(s) 108, 260
PsyI GACNNNGTC 1 cut(s) 590
PvuII CAGCTG 1 cut(s) 809
RsaI GTAC 5 cut(s) 236, 248, 311, 380, 620
RsaNI GTAC 5 cut(s) 235, 247, 310, 379, 619
RseI CAYNNNNRTG 3 cut(s) 281, 374, 675
SacI GAGCTC 2 cut(s) 243, 750
SalI GTCGAC 1 cut(s) 185
SaqAI TTAA 1 cut(s) 801
SatI GCNGC 5 cut(s) 303, 553, 605, 708, 720
Sau3AI GATC 4 cut(s) 57, 108, 260, 478
Sau96I GGNCC 4 cut(s) 361, 460, 680, 717
SchI GAGTC 1 cut(s) 536
ScrFI CCNGG 6 cut(s) 258, 307, 358, 464, 635, 674
SduI GDGCHC 4 cut(s) 46, 243, 574, 750
SexAI ACCWGGT 1 cut(s) 633
SfiI GGCCNNNNNGGCC 1 cut(s) 716
Sfr274I CTCGAG 1 cut(s) 575
SfuI TTCGAA 1 cut(s) 213
SinI GGWCC 2 cut(s) 361, 680
SlaI CTCGAG 1 cut(s) 575
SmiMI CAYNNNNRTG 3 cut(s) 281, 374, 675
SmlI CTYRAG 2 cut(s) 264, 575
SmoI CTYRAG 2 cut(s) 264, 575
SseBI AGGCCT 1 cut(s) 342
SsiI CCGC 9 cut(s) 47, 190, 224, 303, 552, 653, 707, 720, 743
SspMI CTAG 1 cut(s) 296
SstI GAGCTC 2 cut(s) 243, 750
StuI AGGCCT 1 cut(s) 342
StyD4I CCNGG 6 cut(s) 256, 305, 356, 462, 633, 672
StyI CCWWGG 2 cut(s) 279, 738
TaaI ACNGT 1 cut(s) 559
TaiI ACGT 3 cut(s) 294, 327, 549
TaqI TCGA 6 cut(s) 60, 186, 213, 345, 576, 582
TauI GCSGC 4 cut(s) 305, 555, 710, 722
TfiI GAWTC 1 cut(s) 34
Tru1I TTAA 1 cut(s) 801
Tru9I TTAA 1 cut(s) 801
TscAI CASTG 2 cut(s) 158, 690
TseFI GTSAC 2 cut(s) 149, 511
TseI GCWGC 1 cut(s) 604
Tsp45I GTSAC 2 cut(s) 149, 511
TspDTI ATGAA 2 cut(s) 210, 743
TspGWI ACGGA 4 cut(s) 54, 366, 447, 627
TspRI CASTG 2 cut(s) 158, 690
Tth111I GACNNNGTC 1 cut(s) 590
Van91I CCANNNNNTGG 2 cut(s) 523, 640
VpaK11BI GGWCC 2 cut(s) 361, 680
XhoI CTCGAG 1 cut(s) 575
XmiI GTMKAC 1 cut(s) 186
XspI CTAG 1 cut(s) 296
ZraI GACGTC 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.