MD05G1186100.v1.1

4,5-DOPA dioxygenase extradiol-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0017339
Physical Location & Seq
Forward (+)
31463605 .. 31463964
360 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1186100.v1.1.491

Sequence Viewer

Length: 360 bp
ATGTACCAGCTCAAGTACCCTGCCCCCGGCTCCCCCCACCTCGCCAACCGCGTCATGCAGCTGCTTACGTCGTCTGGCTTCGGGCCCGCGGACGTCGATAGCAAGCGGGGGATCAACCACGGCGCGTGGGTCCCGCTCATGATGATGTACCCGGAGAGAGACATCCCGGTTTGCCAGTTATCGATTCAGACAGAGAGGGACGCCGCGTACCACTACAACATGGATAGGGCGCTGGCTCCACTCAAGGACGAGGGCGTGCTTATAGTGGGGTCAGGCAGCGCCACTCACAACTTAAGGGCGTCGGAGAGGGAGATGCGTGGGTCATGGACGCTCTTCTCGAAGGAAGGTACGAGGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00965 map00965
Pfam Domains
Protein Families
LigB

Protein Analysis

120

Amino Acids

13.28

Weight (kDa)

7.93

Isoelectric Point (pI)

33.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LigB PF02900 1 - 104 4.3e-23 Catalytic LigB subunit of aromatic ring-opening dioxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017339)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g30880
malus_domestica MD05G1186100.v1.1
rosa_chinensis RchiOBHm_Chr2g0137351
rosa_laevigata RLG00000019652
rosa_multiflora Rmu_sc0003208.1_g000024
rosa_roxburghii Rroxscaffold_2G00105650 Rroxscaffold_2G00107130
rosa_rugosa Rorug02G0341300
rosa_samantha Rh2AG389800 Rh2BG397300 Rh2CG377300 Rh2DG412500
rosa_wichuraiana Rw2G031930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 96
AccBSI CCGCTC 1 cut(s) 136
AccII CGCG 4 cut(s) 51, 89, 125, 206
AciI CCGC 6 cut(s) 49, 87, 89, 106, 134, 204
AclWI GGATC 1 cut(s) 119
AcyI GRCGYC 3 cut(s) 93, 201, 299
AfaI GTAC 5 cut(s) 5, 17, 149, 209, 349
AfiI CCNNNNNNNGG 1 cut(s) 26
AflII CTTAAG 1 cut(s) 292
AluBI AGCT 2 cut(s) 10, 61
AluI AGCT 2 cut(s) 10, 61
Alw26I GTCTC 1 cut(s) 153
AlwI GGATC 1 cut(s) 119
AoxI GGCC 1 cut(s) 83
ApaI GGGCCC 1 cut(s) 87
ApeKI GCWGC 3 cut(s) 58, 61, 276
AspLEI GCGC 3 cut(s) 125, 232, 281
AspS9I GGNCC 3 cut(s) 83, 84, 130
AsuC2I CCSGG 3 cut(s) 27, 152, 167
AvaII GGWCC 1 cut(s) 130
BaeGI GKGCMC 1 cut(s) 87
BanII GRGCYC 1 cut(s) 87
BbvI GCAGC 3 cut(s) 48, 70, 288
BceAI ACGGC 1 cut(s) 136
BcnI CCSGG 3 cut(s) 27, 152, 167
BcoDI GTCTC 1 cut(s) 153
BfoI RGCGCY 2 cut(s) 233, 282
BfrI CTTAAG 1 cut(s) 292
BisI GCNGC 4 cut(s) 59, 62, 204, 277
BlsI GCNGC 4 cut(s) 60, 63, 205, 278
Bme1390I CCNGG 3 cut(s) 27, 152, 167
Bme18I GGWCC 1 cut(s) 130
BmgT120I GGNCC 3 cut(s) 83, 84, 130
BmiI GGNNCC 5 cut(s) 31, 85, 131, 132, 237
BmrFI CCNGG 3 cut(s) 27, 152, 167
BmsI GCATC 1 cut(s) 303
BpuEI CTTGAG 1 cut(s) 227
BpuMI CCSGG 3 cut(s) 27, 152, 167
Bsa29I ATCGAT 1 cut(s) 182
BsaHI GRCGYC 3 cut(s) 93, 201, 299
BsaJI CCNNGG 3 cut(s) 25, 87, 118
Bsc4I CCNNNNNNNGG 1 cut(s) 26
Bse1I ACTGG 1 cut(s) 175
BseCI ATCGAT 1 cut(s) 182
BseDI CCNNGG 3 cut(s) 25, 87, 118
BseGI GGATG 2 cut(s) 162, 360
BseLI CCNNNNNNNGG 1 cut(s) 26
BseNI ACTGG 1 cut(s) 175
BseSI GKGCMC 1 cut(s) 87
BseXI GCAGC 3 cut(s) 48, 70, 288
Bsh1236I CGCG 4 cut(s) 51, 89, 125, 206
BshFI GGCC 1 cut(s) 85
BshVI ATCGAT 1 cut(s) 182
BsiSI CCGG 3 cut(s) 27, 152, 167
BslFI GGGAC 2 cut(s) 116, 212
BslI CCNNNNNNNGG 1 cut(s) 26
BsmAI GTCTC 1 cut(s) 153
BsmFI GGGAC 2 cut(s) 116, 212
BsnI GGCC 1 cut(s) 85
Bsp120I GGGCCC 1 cut(s) 83
Bsp1286I GDGCHC 1 cut(s) 87
Bsp143I GATC 1 cut(s) 111
BspACI CCGC 6 cut(s) 49, 87, 89, 106, 134, 204
BspANI GGCC 1 cut(s) 85
BspDI ATCGAT 1 cut(s) 182
BspFNI CGCG 4 cut(s) 51, 89, 125, 206
BspHI TCATGA 1 cut(s) 138
BspLI GGNNCC 5 cut(s) 31, 85, 131, 132, 237
BspPI GGATC 1 cut(s) 119
BspQI GCTCTTC 1 cut(s) 338
BspTI CTTAAG 1 cut(s) 292
BsrBI CCGCTC 1 cut(s) 136
BsrI ACTGG 1 cut(s) 175
BssECI CCNNGG 3 cut(s) 25, 87, 118
BssMI GATC 1 cut(s) 111
BssNI GRCGYC 3 cut(s) 93, 201, 299
Bst6I CTCTTC 1 cut(s) 338
BstACI GRCGYC 3 cut(s) 93, 201, 299
BstAFI CTTAAG 1 cut(s) 292
BstC8I GCNNGC 4 cut(s) 87, 104, 234, 257
BstDSI CCRYGG 2 cut(s) 87, 118
BstF5I GGATG 2 cut(s) 162, 360
BstFNI CGCG 4 cut(s) 51, 89, 125, 206
BstH2I RGCGCY 2 cut(s) 233, 282
BstHHI GCGC 3 cut(s) 125, 232, 281
BstKTI GATC 1 cut(s) 114
BstMAI GTCTC 1 cut(s) 153
BstMBI GATC 1 cut(s) 111
BstSCI CCNGG 3 cut(s) 25, 150, 165
BstSLI GKGCMC 1 cut(s) 87
BstUI CGCG 4 cut(s) 51, 89, 125, 206
BstV1I GCAGC 3 cut(s) 48, 70, 288
Bsu15I ATCGAT 1 cut(s) 182
BsuRI GGCC 1 cut(s) 85
BsuTUI ATCGAT 1 cut(s) 182
BtgI CCRYGG 2 cut(s) 87, 118
BtsCI GGATG 2 cut(s) 162, 360
Cac8I GCNNGC 4 cut(s) 87, 104, 234, 257
CciI TCATGA 1 cut(s) 138
CfoI GCGC 3 cut(s) 125, 232, 281
Cfr13I GGNCC 3 cut(s) 83, 84, 130
Cfr42I CCGCGG 1 cut(s) 90
ClaI ATCGAT 1 cut(s) 182
CseI GACGC 4 cut(s) 40, 209, 288, 337
Csp6I GTAC 5 cut(s) 4, 16, 148, 208, 348
CviAII CATG 4 cut(s) 55, 139, 220, 324
CviJI RGCY 6 cut(s) 10, 30, 61, 78, 85, 236
CviKI_1 RGCY 6 cut(s) 10, 30, 61, 78, 85, 236
CviQI GTAC 5 cut(s) 4, 16, 148, 208, 348
DpnI GATC 1 cut(s) 113
DpnII GATC 1 cut(s) 111
Eam1104I CTCTTC 1 cut(s) 338
EarI CTCTTC 1 cut(s) 338
Eco24I GRGCYC 1 cut(s) 87
Eco47I GGWCC 1 cut(s) 130
EcoO109I RGGNCCY 1 cut(s) 130
EcoT38I GRGCYC 1 cut(s) 87
FaeI CATG 4 cut(s) 58, 142, 223, 327
FaiI YATR 5 cut(s) 56, 140, 221, 263, 325
FaqI GGGAC 2 cut(s) 116, 212
FatI CATG 4 cut(s) 54, 138, 219, 323
FauI CCCGC 3 cut(s) 94, 99, 141
Fnu4HI GCNGC 4 cut(s) 59, 62, 204, 277
FokI GGATG 1 cut(s) 149
FriOI GRGCYC 1 cut(s) 87
Fsp4HI GCNGC 4 cut(s) 59, 62, 204, 277
GlaI GCGC 3 cut(s) 124, 231, 280
GluI GCNGC 4 cut(s) 59, 62, 204, 277
HaeII RGCGCY 2 cut(s) 233, 282
HaeIII GGCC 1 cut(s) 85
HapII CCGG 3 cut(s) 27, 152, 167
HgaI GACGC 4 cut(s) 40, 209, 288, 337
HhaI GCGC 3 cut(s) 125, 232, 281
Hin1I GRCGYC 3 cut(s) 93, 201, 299
Hin1II CATG 4 cut(s) 58, 142, 223, 327
Hin6I GCGC 3 cut(s) 123, 230, 279
HinP1I GCGC 3 cut(s) 123, 230, 279
HinfI GANTC 1 cut(s) 184
HpaII CCGG 3 cut(s) 27, 152, 167
Hpy188I TCNGA 2 cut(s) 189, 304
Hpy188III TCNNGA 2 cut(s) 139, 337
Hpy99I CGWCG 3 cut(s) 73, 98, 304
HpyAV CCTTC 2 cut(s) 334, 338
HpyCH4IV ACGT 2 cut(s) 68, 93
HpyCH4V TGCA 1 cut(s) 58
HpySE526I ACGT 2 cut(s) 68, 93
Hsp92I GRCGYC 3 cut(s) 93, 201, 299
Hsp92II CATG 4 cut(s) 58, 142, 223, 327
HspAI GCGC 3 cut(s) 123, 230, 279
KflI GGGWCCC 1 cut(s) 130
KspI CCGCGG 1 cut(s) 90
Kzo9I GATC 1 cut(s) 111
LguI GCTCTTC 1 cut(s) 338
LmnI GCTCC 2 cut(s) 35, 241
LpnPI CCDG 9 cut(s) 20, 33, 40, 60, 165, 180, 188, 218, 258
Lsp1109I GCAGC 3 cut(s) 48, 70, 288
LweI GCATC 1 cut(s) 303
MaeII ACGT 2 cut(s) 68, 93
MalI GATC 1 cut(s) 113
MbiI CCGCTC 1 cut(s) 136
MboI GATC 1 cut(s) 111
MboII GAAGA 1 cut(s) 325
MhlI GDGCHC 1 cut(s) 87
MmeI TCCRAC 1 cut(s) 282
MnlI CCTC 5 cut(s) 50, 189, 244, 300, 345
MseI TTAA 1 cut(s) 293
MslI CAYNNNNRTG 1 cut(s) 143
MspA1I CMGCKG 2 cut(s) 61, 89
MspCI CTTAAG 1 cut(s) 292
MspI CCGG 3 cut(s) 27, 152, 167
MspR9I CCNGG 3 cut(s) 27, 152, 167
MvnI CGCG 4 cut(s) 51, 89, 125, 206
NciI CCSGG 3 cut(s) 27, 152, 167
NdeII GATC 1 cut(s) 111
NlaIII CATG 4 cut(s) 58, 142, 223, 327
NlaIV GGNNCC 5 cut(s) 31, 85, 131, 132, 237
PagI TCATGA 1 cut(s) 138
PciSI GCTCTTC 1 cut(s) 338
PcsI WCGNNNNNNNCGW 2 cut(s) 48, 335
PfeI GAWTC 1 cut(s) 184
PkrI GCNGC 4 cut(s) 60, 63, 205, 278
PpuMI RGGWCCY 1 cut(s) 130
Psp5II RGGWCCY 1 cut(s) 130
PspN4I GGNNCC 5 cut(s) 31, 85, 131, 132, 237
PspOMI GGGCCC 1 cut(s) 83
PspPI GGNCC 3 cut(s) 83, 84, 130
PspPPI RGGWCCY 1 cut(s) 130
PvuII CAGCTG 1 cut(s) 61
RsaI GTAC 5 cut(s) 5, 17, 149, 209, 349
RsaNI GTAC 5 cut(s) 4, 16, 148, 208, 348
RseI CAYNNNNRTG 1 cut(s) 143
SacII CCGCGG 1 cut(s) 90
SapI GCTCTTC 1 cut(s) 338
SaqAI TTAA 1 cut(s) 293
SatI GCNGC 4 cut(s) 59, 62, 204, 277
Sau3AI GATC 1 cut(s) 111
Sau96I GGNCC 3 cut(s) 83, 84, 130
ScrFI CCNGG 3 cut(s) 27, 152, 167
SduI GDGCHC 1 cut(s) 87
SetI ASST 6 cut(s) 12, 42, 63, 71, 96, 349
SfaNI GCATC 1 cut(s) 303
Sfr303I CCGCGG 1 cut(s) 90
SgrBI CCGCGG 1 cut(s) 90
SinI GGWCC 1 cut(s) 130
SmiMI CAYNNNNRTG 1 cut(s) 143
SmlI CTYRAG 3 cut(s) 11, 242, 292
SmoI CTYRAG 3 cut(s) 11, 242, 292
SsiI CCGC 6 cut(s) 49, 87, 89, 106, 134, 204
StyD4I CCNGG 3 cut(s) 25, 150, 165
TaiI ACGT 2 cut(s) 71, 96
TaqI TCGA 3 cut(s) 96, 182, 338
TauI GCSGC 1 cut(s) 206
TfiI GAWTC 1 cut(s) 184
Tru1I TTAA 1 cut(s) 293
Tru9I TTAA 1 cut(s) 293
TseI GCWGC 3 cut(s) 58, 61, 276
Vha464I CTTAAG 1 cut(s) 292
VpaK11BI GGWCC 1 cut(s) 130
ZraI GACGTC 1 cut(s) 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.