Rroxscaffold_2G00105650

4,5-DOPA dioxygenase extradiol-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0017339
Physical Location & Seq
Reverse (-)
29073743 .. 29075361
1619 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00105650.1

Sequence Viewer

Length: 357 bp
ATGTACGAGCTCCAGCACCCAGCTCCGGGATCTCAAGAACTGGACGCCAGGGTGAACGACGTTCTAGTGGCCGCCGGGTACAAGCCTCTCCACGTGGACACAAAGCGAGGCCTAGACCACGGAGCCTGGGTCCCACTCATGCAAATGTACCCTGAAGCGGACGTGCCGGTGTGCCAGCTCTCCATCCAGTCCCACAAGAACGGAACCTATCACTTCAACTTGGGCAAGGCCCTGGCTCCTCTCAGGGAGCAAAGAGTCCTCATCATCGGCACCGGAAGCGTCACCCACAACATGGAGTCCCTCATCGTCAACAACGTCGGCGGCATCGTGGCCGATTGGCCTGGCTCTAGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00965 map00965
Pfam Domains
Protein Families
LigB

Protein Analysis

118

Amino Acids

12.91

Weight (kDa)

6.27

Isoelectric Point (pI)

32.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LigB PF02900 1 - 100 3e-20 Catalytic LigB subunit of aromatic ring-opening dioxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017339)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g30880
malus_domestica MD05G1186100.v1.1
rosa_chinensis RchiOBHm_Chr2g0137351
rosa_laevigata RLG00000019652
rosa_multiflora Rmu_sc0003208.1_g000024
rosa_roxburghii Rroxscaffold_2G00105650 Rroxscaffold_2G00107130
rosa_rugosa Rorug02G0341300
rosa_samantha Rh2AG389800 Rh2BG397300 Rh2CG377300 Rh2DG412500
rosa_wichuraiana Rw2G031930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 269
AccB7I CCANNNNNTGG 1 cut(s) 292
AciI CCGC 3 cut(s) 72, 158, 321
AclWI GGATC 1 cut(s) 37
AcoI YGGCCR 2 cut(s) 69, 330
AcuI CTGAAG 1 cut(s) 174
AcvI CACGTG 1 cut(s) 94
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 3 cut(s) 5, 80, 149
AfiI CCNNNNNNNGG 4 cut(s) 25, 26, 157, 292
AgsI TTSAA 1 cut(s) 217
AjiI CACGTC 1 cut(s) 163
AjnI CCWGG 4 cut(s) 47, 125, 231, 340
AluBI AGCT 3 cut(s) 10, 23, 178
AluI AGCT 3 cut(s) 10, 23, 178
Alw21I GWGCWC 1 cut(s) 12
AlwI GGATC 1 cut(s) 37
AoxI GGCC 5 cut(s) 69, 109, 228, 330, 338
AspS9I GGNCC 2 cut(s) 130, 229
AsuC2I CCSGG 2 cut(s) 27, 76
AsuHPI GGTGA 2 cut(s) 64, 274
AvaII GGWCC 1 cut(s) 130
BanI GGYRCC 1 cut(s) 269
BanII GRGCYC 1 cut(s) 12
BbrPI CACGTG 1 cut(s) 94
Bbv12I GWGCWC 1 cut(s) 12
BccI CCATC 1 cut(s) 191
BciT130I CCWGG 4 cut(s) 49, 127, 233, 342
BcnI CCSGG 2 cut(s) 27, 76
BfaI CTAG 3 cut(s) 65, 113, 348
BisI GCNGC 2 cut(s) 72, 322
BlsI GCNGC 2 cut(s) 73, 323
Bme1390I CCNGG 6 cut(s) 27, 49, 76, 127, 233, 342
Bme18I GGWCC 1 cut(s) 130
BmgBI CACGTC 1 cut(s) 163
BmgT120I GGNCC 2 cut(s) 130, 229
BmiI GGNNCC 6 cut(s) 124, 131, 132, 205, 237, 271
BmrFI CCNGG 6 cut(s) 27, 49, 76, 127, 233, 342
BmsI GCATC 1 cut(s) 333
BpuEI CTTGAG 1 cut(s) 18
BpuMI CCSGG 2 cut(s) 27, 76
BsaAI YACGTR 1 cut(s) 94
BsaHI GRCGYC 1 cut(s) 45
BsaJI CCNNGG 4 cut(s) 48, 118, 126, 231
BsaWI WCCGGW 1 cut(s) 272
BsaXI ACNNNNNCTCC 2 cut(s) 114, 144
Bsc4I CCNNNNNNNGG 4 cut(s) 25, 26, 157, 292
Bse118I RCCGGY 1 cut(s) 166
Bse1I ACTGG 2 cut(s) 45, 187
BseBI CCWGG 4 cut(s) 49, 127, 233, 342
BseDI CCNNGG 4 cut(s) 48, 118, 126, 231
BseGI GGATG 1 cut(s) 183
BseLI CCNNNNNNNGG 4 cut(s) 25, 26, 157, 292
BseMII CTCAG 1 cut(s) 256
BseNI ACTGG 2 cut(s) 45, 187
BseRI GAGGAG 1 cut(s) 228
BseYI CCCAGC 1 cut(s) 19
BshFI GGCC 5 cut(s) 71, 111, 230, 332, 340
BshNI GGYRCC 1 cut(s) 269
BsiHKAI GWGCWC 1 cut(s) 12
BsiSI CCGG 4 cut(s) 26, 75, 167, 273
BslFI GGGAC 3 cut(s) 116, 175, 283
BslI CCNNNNNNNGG 4 cut(s) 25, 26, 157, 292
BsmFI GGGAC 3 cut(s) 116, 175, 283
BsnI GGCC 5 cut(s) 71, 111, 230, 332, 340
Bsp1286I GDGCHC 1 cut(s) 12
Bsp143I GATC 1 cut(s) 29
BspACI CCGC 3 cut(s) 72, 158, 321
BspANI GGCC 5 cut(s) 71, 111, 230, 332, 340
BspCNI CTCAG 1 cut(s) 255
BspLI GGNNCC 6 cut(s) 124, 131, 132, 205, 237, 271
BspPI GGATC 1 cut(s) 37
BspT107I GGYRCC 1 cut(s) 269
BsrFI RCCGGY 1 cut(s) 166
BsrI ACTGG 2 cut(s) 45, 187
BssAI RCCGGY 1 cut(s) 166
BssECI CCNNGG 4 cut(s) 48, 118, 126, 231
BssMI GATC 1 cut(s) 29
BssNI GRCGYC 1 cut(s) 45
Bst2UI CCWGG 4 cut(s) 49, 127, 233, 342
BstACI GRCGYC 1 cut(s) 45
BstBAI YACGTR 1 cut(s) 94
BstC8I GCNNGC 1 cut(s) 176
BstDEI CTNAG 1 cut(s) 242
BstDSI CCRYGG 1 cut(s) 118
BstF5I GGATG 1 cut(s) 183
BstKTI GATC 1 cut(s) 32
BstMBI GATC 1 cut(s) 29
BstMWI GCNNNNNNNGC 1 cut(s) 276
BstNI CCWGG 4 cut(s) 49, 127, 233, 342
BstSCI CCNGG 6 cut(s) 25, 47, 74, 125, 231, 340
BstX2I RGATCY 1 cut(s) 29
BstYI RGATCY 1 cut(s) 29
BsuRI GGCC 5 cut(s) 71, 111, 230, 332, 340
BtgI CCRYGG 1 cut(s) 118
BtrI CACGTC 1 cut(s) 163
BtsCI GGATG 1 cut(s) 183
Cac8I GCNNGC 1 cut(s) 176
Cfr10I RCCGGY 1 cut(s) 166
Cfr13I GGNCC 2 cut(s) 130, 229
CseI GACGC 2 cut(s) 53, 268
Csp6I GTAC 3 cut(s) 4, 79, 148
CviAII CATG 2 cut(s) 139, 292
CviQI GTAC 3 cut(s) 4, 79, 148
DdeI CTNAG 1 cut(s) 242
DpnI GATC 1 cut(s) 31
DpnII GATC 1 cut(s) 29
EaeI YGGCCR 2 cut(s) 69, 330
Ecl136II GAGCTC 1 cut(s) 10
Eco147I AGGCCT 1 cut(s) 111
Eco24I GRGCYC 1 cut(s) 12
Eco47I GGWCC 1 cut(s) 130
Eco53kI GAGCTC 1 cut(s) 10
Eco57I CTGAAG 1 cut(s) 174
Eco72I CACGTG 1 cut(s) 94
EcoICRI GAGCTC 1 cut(s) 10
EcoO109I RGGNCCY 2 cut(s) 130, 229
EcoRII CCWGG 4 cut(s) 47, 125, 231, 340
EcoT38I GRGCYC 1 cut(s) 12
FaeI CATG 2 cut(s) 142, 295
FaiI YATR 2 cut(s) 140, 293
FaqI GGGAC 3 cut(s) 116, 175, 283
FatI CATG 2 cut(s) 138, 291
Fnu4HI GCNGC 2 cut(s) 72, 322
FokI GGATG 1 cut(s) 170
FriOI GRGCYC 1 cut(s) 12
Fsp4HI GCNGC 2 cut(s) 72, 322
FspBI CTAG 3 cut(s) 65, 113, 348
GluI GCNGC 2 cut(s) 72, 322
GsaI CCCAGC 1 cut(s) 23
HaeIII GGCC 5 cut(s) 71, 111, 230, 332, 340
HapII CCGG 4 cut(s) 26, 75, 167, 273
HgaI GACGC 2 cut(s) 53, 268
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 2 cut(s) 142, 295
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HinfI GANTC 2 cut(s) 255, 296
HpaII CCGG 4 cut(s) 26, 75, 167, 273
HphI GGTGA 2 cut(s) 64, 274
Hpy166II GTNNAC 3 cut(s) 55, 97, 310
Hpy188III TCNNGA 2 cut(s) 35, 348
Hpy8I GTNNAC 3 cut(s) 55, 97, 310
Hpy99I CGWCG 2 cut(s) 62, 320
HpyCH4IV ACGT 4 cut(s) 60, 93, 162, 315
HpyCH4V TGCA 1 cut(s) 142
HpyF10VI GCNNNNNNNGC 1 cut(s) 276
HpyF3I CTNAG 1 cut(s) 242
HpySE526I ACGT 4 cut(s) 60, 93, 162, 315
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 2 cut(s) 142, 295
KflI GGGWCCC 1 cut(s) 130
Kzo9I GATC 1 cut(s) 29
LmnI GCTCC 5 cut(s) 15, 28, 122, 241, 247
LweI GCATC 1 cut(s) 333
MaeI CTAG 3 cut(s) 65, 113, 348
MaeII ACGT 4 cut(s) 60, 93, 162, 315
MaeIII GTNAC 1 cut(s) 280
MalI GATC 1 cut(s) 31
MboI GATC 1 cut(s) 29
MflI RGATCY 1 cut(s) 29
MhlI GDGCHC 1 cut(s) 12
MlyI GAGTC 2 cut(s) 264, 305
MnlI CCTC 5 cut(s) 96, 101, 249, 269, 311
MslI CAYNNNNRTG 1 cut(s) 143
MspI CCGG 4 cut(s) 26, 75, 167, 273
MspR9I CCNGG 6 cut(s) 27, 49, 76, 127, 233, 342
MvaI CCWGG 4 cut(s) 49, 127, 233, 342
MwoI GCNNNNNNNGC 1 cut(s) 276
NciI CCSGG 2 cut(s) 27, 76
NdeII GATC 1 cut(s) 29
NlaIII CATG 2 cut(s) 142, 295
NlaIV GGNNCC 6 cut(s) 124, 131, 132, 205, 237, 271
NmuCI GTSAC 1 cut(s) 280
PceI AGGCCT 1 cut(s) 111
PcsI WCGNNNNNNNCGW 2 cut(s) 312, 324
PflMI CCANNNNNTGG 1 cut(s) 292
PfoI TCCNGGA 1 cut(s) 25
PkrI GCNGC 2 cut(s) 73, 323
PleI GAGTC 2 cut(s) 263, 304
PmaCI CACGTG 1 cut(s) 94
PmlI CACGTG 1 cut(s) 94
PpsI GAGTC 2 cut(s) 263, 304
Ppu21I YACGTR 1 cut(s) 94
PpuMI RGGWCCY 1 cut(s) 130
Psp124BI GAGCTC 1 cut(s) 12
Psp5II RGGWCCY 1 cut(s) 130
Psp6I CCWGG 4 cut(s) 47, 125, 231, 340
PspCI CACGTG 1 cut(s) 94
PspFI CCCAGC 1 cut(s) 19
PspGI CCWGG 4 cut(s) 47, 125, 231, 340
PspN4I GGNNCC 6 cut(s) 124, 131, 132, 205, 237, 271
PspPI GGNCC 2 cut(s) 130, 229
PspPPI RGGWCCY 1 cut(s) 130
PsuI RGATCY 1 cut(s) 29
RsaI GTAC 3 cut(s) 5, 80, 149
RsaNI GTAC 3 cut(s) 4, 79, 148
RseI CAYNNNNRTG 1 cut(s) 143
SacI GAGCTC 1 cut(s) 12
SatI GCNGC 2 cut(s) 72, 322
Sau3AI GATC 1 cut(s) 29
Sau96I GGNCC 2 cut(s) 130, 229
SchI GAGTC 2 cut(s) 264, 305
ScrFI CCNGG 6 cut(s) 27, 49, 76, 127, 233, 342
SduI GDGCHC 1 cut(s) 12
SetI ASST 8 cut(s) 12, 25, 63, 96, 165, 180, 209, 318
SfaNI GCATC 1 cut(s) 333
SinI GGWCC 1 cut(s) 130
SmiMI CAYNNNNRTG 1 cut(s) 143
SmlI CTYRAG 1 cut(s) 33
SmoI CTYRAG 1 cut(s) 33
SseBI AGGCCT 1 cut(s) 111
SsiI CCGC 3 cut(s) 72, 158, 321
SspMI CTAG 3 cut(s) 65, 113, 348
SstI GAGCTC 1 cut(s) 12
StuI AGGCCT 1 cut(s) 111
StyD4I CCNGG 6 cut(s) 25, 47, 74, 125, 231, 340
TaiI ACGT 4 cut(s) 63, 96, 165, 318
TauI GCSGC 2 cut(s) 74, 324
TseFI GTSAC 1 cut(s) 280
Tsp45I GTSAC 1 cut(s) 280
TspGWI ACGGA 2 cut(s) 135, 216
Van91I CCANNNNNTGG 1 cut(s) 292
VpaK11BI GGWCC 1 cut(s) 130
XbaI TCTAGA 1 cut(s) 347
XspI CTAG 3 cut(s) 65, 113, 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.