Rorug02G0341300

4,5-DOPA dioxygenase extradiol-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0017339
Physical Location & Seq
Reverse (-)
42476727 .. 42480801
4075 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0341300.1

Sequence Viewer

Length: 1284 bp
ATGGCTGATCCTGAGATGATTGAGATTTCTGTTGAAGCGGATGATGACCAAAGTACTACTGATGGAAGTAAAGATATTCGATCTAAAAGCTCTTCTCCGAAAGCATCATGTTTGTTCTTTGACTTGAATGAGGATGCAAGTAGTGATGATCATGGGGATGTTTCAAACAATATAGGTGAAGCTAGGGAAAGCAAAACATCTACAGAAGGGAAAGAACAGACTGCAACCAGCAGCGGTGCAGTCAGGCAATATGTTCGATCAAAAATGCCAAGGCTTCGTTGGACTTCTGATCTCCATCTAGCATTCATTCATGCCGTTGAGAGGCTCGGAGGACAAGAAAGAGCAACTCCAAAATTAGTACTTCAGTTGATGAATGTGAGAGGACTCAGCATTGCTCACGTCAAGAGTCATTTGCAGATGTATCGAAGCAAGAAGCTCGATGAGTCCGGCCAAGTGTTACCACAGTTCAATAGATCGAGGGGAATGTTGTTGCAAGGAGGAGAGCACATTGTGGAAGCCTATCAACAGTACAATCCTTTCAGGCACTTCAGACTTGATGACAGAGGAAGCCATTTTCCTTCTCCACCATTAAAACATCAGCCATATGATAATTTGAATGCCAACTCTTCAAGGTTGATTTCACAGTGGAGCAATTCTGGGATGGAGAACCATCGGAGCATCAATACAATTACAAGCTCACATATGTTTAGTGTAAAGGAAGCCATGACAAGAGTTAATGGACCTATTAGATCCAGCCGGTTTCTTGAAGAGAAGAGATGGCCTCCTCGTGAGATGATAGGAAATCACATTAGAGGCAGGAGACATTCTCTAAACATTTCTTGGGTTAATACCATCAATGTCGCACAACCTAATTCAGTAGGGAGTCCCCAGGCAAGCTCCAACCGACATCTACCCAATCATACTACATGTTCTCCAAAGTTCATCTCCAGCAGCTACGAACCCTTATCTGATCCGCACGAGGTTCGGAAACCTGATGAAAGCATAAATGGCGAAACATTTGGAGCAGAAGTTGGTGATAATATGCAAACTTTGAAACAAAAGAAATGGTTACCTTTGAATTTACAGTTGGGCCTGCGGCAAGAATTCAGTATCTGCAGTGACGAGATGAAGGATGGTGAACAAGAAAGCACGAGAGACATTGAAACAACGCTTTCTCTATCTTTATCCTCGTCTTCACCAAGGCAACAAGATCAATCCAGTACACCCACATCAAGAGTTCGACACTTGTCATTTCCTCTCTCTCCTGGCCTTAAACCTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00965 map00965
Pfam Domains
Protein Families
LigB

Protein Analysis

427

Amino Acids

47.98

Weight (kDa)

7.84

Isoelectric Point (pI)

61.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 91 - 142 2.2e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017339)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g30880
malus_domestica MD05G1186100.v1.1
rosa_chinensis RchiOBHm_Chr2g0137351
rosa_laevigata RLG00000019652
rosa_multiflora Rmu_sc0003208.1_g000024
rosa_roxburghii Rroxscaffold_2G00105650 Rroxscaffold_2G00107130
rosa_rugosa Rorug02G0341300
rosa_samantha Rh2AG389800 Rh2BG397300 Rh2CG377300 Rh2DG412500
rosa_wichuraiana Rw2G031930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 38, 234, 974, 1096
AclWI GGATC 3 cut(s) 2, 744, 965
AcoI YGGCCR 1 cut(s) 448
AcsI RAATTY 2 cut(s) 1078, 1103
AcuI CTGAAG 2 cut(s) 347, 532
AdeI CACNNNGTG 1 cut(s) 511
AfaI GTAC 4 cut(s) 55, 360, 530, 1222
AfiI CCNNNNNNNGG 1 cut(s) 321
AflIII ACRYGT 1 cut(s) 926
AjiI CACGTC 1 cut(s) 400
AjnI CCWGG 2 cut(s) 888, 1264
AjuI GAANNNNNNNTTGG 2 cut(s) 1070, 1102
AluBI AGCT 6 cut(s) 90, 182, 436, 696, 897, 954
AluI AGCT 6 cut(s) 90, 182, 436, 696, 897, 954
Alw21I GWGCWC 1 cut(s) 507
Alw26I GTCTC 2 cut(s) 814, 1149
AlwI GGATC 3 cut(s) 2, 744, 965
AlwNI CAGNNNCTG 1 cut(s) 1113
AoxI GGCC 4 cut(s) 448, 779, 1090, 1267
ApeKI GCWGC 2 cut(s) 231, 951
ApoI RAATTY 2 cut(s) 1078, 1103
AspS9I GGNCC 2 cut(s) 740, 1090
AsuHPI GGTGA 4 cut(s) 188, 1046, 1148, 1188
AvaII GGWCC 1 cut(s) 740
BauI CACGAG 3 cut(s) 786, 977, 1150
BbsI GAAGAC 1 cut(s) 1185
Bbv12I GWGCWC 1 cut(s) 507
BbvI GCAGC 2 cut(s) 243, 963
BccI CCATC 7 cut(s) 56, 303, 655, 678, 771, 860, 1127
BceAI ACGGC 1 cut(s) 299
BcgI CGANNNNNNTGC 8 cut(s) 236, 270, 404, 418, 438, 452, 965, 999
BciT130I CCWGG 2 cut(s) 890, 1266
BclI TGATCA 1 cut(s) 148
BcoDI GTCTC 2 cut(s) 814, 1149
BfaI CTAG 2 cut(s) 183, 299
BfmI CTRYAG 2 cut(s) 201, 1114
BisI GCNGC 3 cut(s) 232, 952, 1097
BlsI GCNGC 3 cut(s) 233, 953, 1098
BmcAI AGTACT 2 cut(s) 55, 360
Bme1390I CCNGG 2 cut(s) 890, 1266
Bme18I GGWCC 1 cut(s) 740
BmgBI CACGTC 1 cut(s) 400
BmgT120I GGNCC 2 cut(s) 740, 1090
BmrFI CCNGG 2 cut(s) 890, 1266
BmsI GCATC 3 cut(s) 113, 124, 687
BoxI GACNNNNGTC 1 cut(s) 1246
BpiI GAAGAC 1 cut(s) 1185
BplI GAGNNNNNCTC 4 cut(s) 766, 798, 811, 843
BpmI CTGGAG 1 cut(s) 931
BsaBI GATNNNNATC 1 cut(s) 294
BsaJI CCNNGG 3 cut(s) 269, 888, 1199
BsaXI ACNNNNNCTCC 4 cut(s) 916, 946, 1014, 1044
Bsc4I CCNNNNNNNGG 1 cut(s) 321
Bse118I RCCGGY 1 cut(s) 756
Bse1I ACTGG 1 cut(s) 1218
Bse3DI GCAATG 1 cut(s) 390
Bse8I GATNNNNATC 1 cut(s) 294
BseBI CCWGG 2 cut(s) 890, 1266
BseDI CCNNGG 3 cut(s) 269, 888, 1199
BseGI GGATG 5 cut(s) 46, 139, 163, 666, 1138
BseJI GATNNNNATC 1 cut(s) 294
BseLI CCNNNNNNNGG 1 cut(s) 321
BseMI GCAATG 1 cut(s) 390
BseMII CTCAG 1 cut(s) 400
BseNI ACTGG 1 cut(s) 1218
BseRI GAGGAG 2 cut(s) 513, 774
BseXI GCAGC 2 cut(s) 243, 963
BsgI GTGCAG 1 cut(s) 258
BshFI GGCC 4 cut(s) 450, 781, 1092, 1269
BsiHKAI GWGCWC 1 cut(s) 507
BsiSI CCGG 2 cut(s) 447, 757
BslFI GGGAC 1 cut(s) 870
BslI CCNNNNNNNGG 1 cut(s) 321
BsmAI GTCTC 2 cut(s) 814, 1149
BsmFI GGGAC 1 cut(s) 870
BsmI GAATGC 2 cut(s) 302, 622
BsnI GGCC 4 cut(s) 450, 781, 1092, 1269
Bsp1286I GDGCHC 1 cut(s) 507
Bsp143I GATC 9 cut(s) 7, 80, 148, 257, 289, 473, 749, 970, 1210
BspACI CCGC 4 cut(s) 38, 234, 974, 1096
BspANI GGCC 4 cut(s) 450, 781, 1092, 1269
BspCNI CTCAG 2 cut(s) 4, 399
BspMAI CTGCAG 1 cut(s) 1118
BspPI GGATC 3 cut(s) 2, 744, 965
BspQI GCTCTTC 1 cut(s) 97
BsrDI GCAATG 1 cut(s) 390
BsrFI RCCGGY 1 cut(s) 756
BsrI ACTGG 1 cut(s) 1218
BssAI RCCGGY 1 cut(s) 756
BssECI CCNNGG 3 cut(s) 269, 888, 1199
BssMI GATC 9 cut(s) 7, 80, 148, 257, 289, 473, 749, 970, 1210
BssSI CACGAG 3 cut(s) 786, 977, 1150
BssT1I CCWWGG 2 cut(s) 269, 1199
Bst2BI CACGAG 3 cut(s) 786, 977, 1150
Bst2UI CCWGG 2 cut(s) 890, 1266
Bst4CI ACNGT 4 cut(s) 465, 528, 645, 1086
Bst6I CTCTTC 4 cut(s) 97, 631, 762, 767
BstC8I GCNNGC 2 cut(s) 895, 1094
BstDEI CTNAG 2 cut(s) 12, 386
BstEII GGTNACC 1 cut(s) 1068
BstF5I GGATG 5 cut(s) 46, 139, 163, 666, 1138
BstKTI GATC 9 cut(s) 10, 83, 151, 260, 292, 476, 752, 973, 1213
BstMAI GTCTC 2 cut(s) 814, 1149
BstMBI GATC 9 cut(s) 7, 80, 148, 257, 289, 473, 749, 970, 1210
BstMWI GCNNNNNNNGC 1 cut(s) 1008
BstNI CCWGG 2 cut(s) 890, 1266
BstNSI RCATGY 1 cut(s) 930
BstPAI GACNNNNGTC 1 cut(s) 1246
BstPI GGTNACC 1 cut(s) 1068
BstSCI CCNGG 2 cut(s) 888, 1264
BstSFI CTRYAG 2 cut(s) 201, 1114
BstV1I GCAGC 2 cut(s) 243, 963
BstV2I GAAGAC 1 cut(s) 1185
BstX2I RGATCY 1 cut(s) 749
BstYI RGATCY 1 cut(s) 749
BsuRI GGCC 4 cut(s) 450, 781, 1092, 1269
BtrI CACGTC 1 cut(s) 400
BtsCI GGATG 5 cut(s) 46, 139, 163, 666, 1138
BtsI GCAGTG 1 cut(s) 1123
BtsIMutI CAGTG 2 cut(s) 650, 1123
Cac8I GCNNGC 2 cut(s) 895, 1094
CaiI CAGNNNCTG 1 cut(s) 1113
Cfr10I RCCGGY 1 cut(s) 756
Cfr13I GGNCC 2 cut(s) 740, 1090
Csp6I GTAC 4 cut(s) 54, 359, 529, 1221
CviAII CATG 5 cut(s) 108, 152, 311, 724, 927
CviQI GTAC 4 cut(s) 54, 359, 529, 1221
DdeI CTNAG 2 cut(s) 12, 386
DpnI GATC 9 cut(s) 9, 82, 150, 259, 291, 475, 751, 972, 1212
DpnII GATC 9 cut(s) 7, 80, 148, 257, 289, 473, 749, 970, 1210
DraIII CACNNNGTG 1 cut(s) 511
EaeI YGGCCR 1 cut(s) 448
Eam1104I CTCTTC 4 cut(s) 97, 631, 762, 767
EarI CTCTTC 4 cut(s) 97, 631, 762, 767
Eco130I CCWWGG 2 cut(s) 269, 1199
Eco47I GGWCC 1 cut(s) 740
Eco57I CTGAAG 2 cut(s) 347, 532
Eco91I GGTNACC 1 cut(s) 1068
EcoO65I GGTNACC 1 cut(s) 1068
EcoRI GAATTC 1 cut(s) 1103
EcoRII CCWGG 2 cut(s) 888, 1264
EcoT14I CCWWGG 2 cut(s) 269, 1199
ErhI CCWWGG 2 cut(s) 269, 1199
FaeI CATG 5 cut(s) 111, 155, 314, 727, 930
FaqI GGGAC 1 cut(s) 870
FatI CATG 5 cut(s) 107, 151, 310, 723, 926
FauNDI CATATG 2 cut(s) 604, 702
FbaI TGATCA 1 cut(s) 148
Fnu4HI GCNGC 3 cut(s) 232, 952, 1097
FokI GGATG 5 cut(s) 53, 146, 170, 673, 1145
Fsp4HI GCNGC 3 cut(s) 232, 952, 1097
FspBI CTAG 2 cut(s) 183, 299
GluI GCNGC 3 cut(s) 232, 952, 1097
GsuI CTGGAG 1 cut(s) 931
HaeIII GGCC 4 cut(s) 450, 781, 1092, 1269
HapII CCGG 2 cut(s) 447, 757
Hin1II CATG 5 cut(s) 111, 155, 314, 727, 930
HinfI GANTC 4 cut(s) 384, 406, 443, 883
HpaII CCGG 2 cut(s) 447, 757
HphI GGTGA 4 cut(s) 188, 1046, 1148, 1188
Hpy166II GTNNAC 2 cut(s) 1139, 1223
Hpy188I TCNGA 7 cut(s) 99, 289, 329, 551, 675, 970, 987
Hpy188III TCNNGA 5 cut(s) 11, 403, 764, 788, 1233
Hpy8I GTNNAC 2 cut(s) 1139, 1223
HpyAV CCTTC 3 cut(s) 200, 588, 1123
HpyCH4III ACNGT 4 cut(s) 465, 528, 645, 1086
HpyCH4IV ACGT 1 cut(s) 399
HpyCH4V TGCA 7 cut(s) 137, 224, 239, 415, 493, 1045, 1116
HpyF10VI GCNNNNNNNGC 1 cut(s) 1008
HpyF3I CTNAG 2 cut(s) 12, 386
HpySE526I ACGT 1 cut(s) 399
Hsp92II CATG 5 cut(s) 111, 155, 314, 727, 930
Ksp22I TGATCA 1 cut(s) 148
Kzo9I GATC 9 cut(s) 7, 80, 148, 257, 289, 473, 749, 970, 1210
LguI GCTCTTC 1 cut(s) 97
LmnI GCTCC 4 cut(s) 648, 675, 902, 1022
Lsp1109I GCAGC 2 cut(s) 243, 963
LweI GCATC 3 cut(s) 113, 124, 687
MaeI CTAG 2 cut(s) 183, 299
MaeII ACGT 1 cut(s) 399
MaeIII GTNAC 3 cut(s) 456, 1068, 1118
MalI GATC 9 cut(s) 9, 82, 150, 259, 291, 475, 751, 972, 1212
MboI GATC 9 cut(s) 7, 80, 148, 257, 289, 473, 749, 970, 1210
MboII GAAGA 5 cut(s) 84, 618, 779, 784, 1185
MflI RGATCY 1 cut(s) 749
MhlI GDGCHC 1 cut(s) 507
MluCI AATT 7 cut(s) 353, 610, 652, 687, 871, 1078, 1103
MlyI GAGTC 4 cut(s) 378, 415, 452, 892
MmeI TCCRAC 2 cut(s) 260, 924
MseI TTAA 5 cut(s) 590, 735, 846, 1272, 1282
MslI CAYNNNNRTG 1 cut(s) 156
MspA1I CMGCKG 1 cut(s) 234
MspI CCGG 2 cut(s) 447, 757
MspR9I CCNGG 2 cut(s) 890, 1266
Mva1269I GAATGC 2 cut(s) 302, 622
MvaI CCWGG 2 cut(s) 890, 1266
MwoI GCNNNNNNNGC 1 cut(s) 1008
NdeI CATATG 2 cut(s) 604, 702
NdeII GATC 9 cut(s) 7, 80, 148, 257, 289, 473, 749, 970, 1210
NlaIII CATG 5 cut(s) 111, 155, 314, 727, 930
NmuCI GTSAC 1 cut(s) 1118
NspI RCATGY 1 cut(s) 930
PciI ACATGT 1 cut(s) 926
PciSI GCTCTTC 1 cut(s) 97
PctI GAATGC 2 cut(s) 302, 622
PkrI GCNGC 3 cut(s) 233, 953, 1098
PleI GAGTC 4 cut(s) 378, 414, 451, 891
PpsI GAGTC 4 cut(s) 378, 414, 451, 891
PscI ACATGT 1 cut(s) 926
PshAI GACNNNNGTC 1 cut(s) 1246
Psp6I CCWGG 2 cut(s) 888, 1264
PspEI GGTNACC 1 cut(s) 1068
PspGI CCWGG 2 cut(s) 888, 1264
PspPI GGNCC 2 cut(s) 740, 1090
PstI CTGCAG 1 cut(s) 1118
PstNI CAGNNNCTG 1 cut(s) 1113
PsuI RGATCY 1 cut(s) 749
RsaI GTAC 4 cut(s) 55, 360, 530, 1222
RsaNI GTAC 4 cut(s) 54, 359, 529, 1221
RseI CAYNNNNRTG 1 cut(s) 156
SapI GCTCTTC 1 cut(s) 97
SaqAI TTAA 5 cut(s) 590, 735, 846, 1272, 1282
SatI GCNGC 3 cut(s) 232, 952, 1097
Sau3AI GATC 9 cut(s) 7, 80, 148, 257, 289, 473, 749, 970, 1210
Sau96I GGNCC 2 cut(s) 740, 1090
ScaI AGTACT 2 cut(s) 55, 360
SchI GAGTC 4 cut(s) 378, 415, 452, 892
ScrFI CCNGG 2 cut(s) 890, 1266
SduI GDGCHC 1 cut(s) 507
SfaNI GCATC 3 cut(s) 113, 124, 687
SfcI CTRYAG 2 cut(s) 201, 1114
SinI GGWCC 1 cut(s) 740
SmiMI CAYNNNNRTG 1 cut(s) 156
Sse9I AATT 7 cut(s) 353, 610, 652, 687, 871, 1078, 1103
SsiI CCGC 4 cut(s) 38, 234, 974, 1096
SspMI CTAG 2 cut(s) 183, 299
StyD4I CCNGG 2 cut(s) 888, 1264
StyI CCWWGG 2 cut(s) 269, 1199
TaaI ACNGT 4 cut(s) 465, 528, 645, 1086
TaiI ACGT 1 cut(s) 402
TaqI TCGA 6 cut(s) 79, 256, 424, 438, 476, 1240
TasI AATT 7 cut(s) 353, 610, 652, 687, 871, 1078, 1103
TatI WGTACW 4 cut(s) 53, 358, 528, 1220
TauI GCSGC 1 cut(s) 1099
Tru1I TTAA 5 cut(s) 590, 735, 846, 1272, 1282
Tru9I TTAA 5 cut(s) 590, 735, 846, 1272, 1282
TscAI CASTG 2 cut(s) 650, 1123
TseFI GTSAC 1 cut(s) 1118
TseI GCWGC 2 cut(s) 231, 951
Tsp45I GTSAC 1 cut(s) 1118
TspDTI ATGAA 6 cut(s) 295, 299, 386, 931, 1011, 1142
TspRI CASTG 2 cut(s) 650, 1123
VpaK11BI GGWCC 1 cut(s) 740
XapI RAATTY 2 cut(s) 1078, 1103
XceI RCATGY 1 cut(s) 930
XcmI CCANNNNNNNNNTGG 1 cut(s) 276
XspI CTAG 2 cut(s) 183, 299
ZrmI AGTACT 2 cut(s) 55, 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.