FvH4_6g32260

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0015897
Physical Location & Seq
Reverse (-)
25295158 .. 25295727
570 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g32260.t1

Sequence Viewer

Length: 570 bp
ATGGCGCCTCATGGAGAAGCTATTGCTACCAATTACTCTACCTCCACCAGAAACATCTCCAAATCCAAACCACCAAGGCTCTCATTCTCATCCGATAGCCTTAAAAGAACCATATCGGATATCTCTTTCGAACTCAGCAAGGAGAGTTCTATCGAGGATATCAAAGCACTTCCACCCATTTCGGAGGTTGAAGACGCGAGGTGTGAGTGCTGTGGCATGAGCGAAGAGTGCACACCCGAGTACATAGACCGAGTGCGAGACAAGTACTTGGGGAAGTGGATCTGCGGGTTGTGTGCCGAGGCAGTGAAAGAAGAGCTGGAGAAAAATGGAGGAAACAAAGAAGAGGCCTTGAATGCACACATAAGTGCATGTGTAAGGTTTAACAAGTATAGCAGGGCTTATCCAGTTTTGTTCCAAGCTGAAGCTATGAAAGATATGTTGAAGAAGAGCAAACTGGAAGGTAAAAGTGTGAGGGCCAAGTCCATCAGTCCTAGGGACAAAGGAACAAGGAAGGGTGTGATTGCTCGGAGTTCGAGCTGCATTCCGGCAATTACTAGGGAAATGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

190

Amino Acids

21.02

Weight (kDa)

8.86

Isoelectric Point (pI)

53.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 63 - 151 2.6e-36 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015897)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79770
fragaria_vesca FvH4_6g32260
malus_domestica MD09G1205300.v1.1 MD17G1185200.v1.1
prunus_persica Prupe.3G060600_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0140141
rosa_laevigata RLG00000019855
rosa_multiflora Rmu_sc0003244.1_g000018
rosa_roxburghii Rroxscaffold_2G00105010
rosa_rugosa Rorug02G0361400
rosa_samantha Rh2AG412100 Rh2BG422500 Rh2CG398200 Rh2DG431800
rosa_wichuraiana Rw2G033770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccII CGCG 1 cut(s) 197
AciI CCGC 1 cut(s) 285
AclWI GGATC 1 cut(s) 287
AcuI CTGAAG 1 cut(s) 441
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 242, 266
AgsI TTSAA 3 cut(s) 191, 352, 442
AleI CACNNNNGTG 1 cut(s) 363
AluBI AGCT 5 cut(s) 20, 316, 419, 425, 537
AluI AGCT 5 cut(s) 20, 316, 419, 425, 537
Alw21I GWGCWC 1 cut(s) 233
Alw26I GTCTC 1 cut(s) 252
Alw44I GTGCAC 1 cut(s) 229
AlwI GGATC 1 cut(s) 287
Ama87I CYCGRG 1 cut(s) 236
AoxI GGCC 2 cut(s) 345, 474
ApaLI GTGCAC 1 cut(s) 229
ApeKI GCWGC 1 cut(s) 537
AspA2I CCTAGG 1 cut(s) 491
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 1 cut(s) 474
AsuII TTCGAA 1 cut(s) 129
AvaI CYCGRG 1 cut(s) 236
AvrII CCTAGG 1 cut(s) 491
BaeGI GKGCMC 1 cut(s) 233
BanI GGYRCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 198
Bbv12I GWGCWC 1 cut(s) 233
BbvI GCAGC 1 cut(s) 524
BccI CCATC 1 cut(s) 491
BcoDI GTCTC 1 cut(s) 252
BfaI CTAG 2 cut(s) 492, 555
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 1 cut(s) 538
BlnI CCTAGG 1 cut(s) 491
BlsI GCNGC 1 cut(s) 539
BmcAI AGTACT 1 cut(s) 266
BmeT110I CYCGRG 1 cut(s) 236
BmgT120I GGNCC 1 cut(s) 474
BmiI GGNNCC 1 cut(s) 6
BpiI GAAGAC 1 cut(s) 198
BpmI CTGGAG 1 cut(s) 338
Bpu14I TTCGAA 1 cut(s) 129
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 74, 297, 491
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
Bse1I ACTGG 2 cut(s) 404, 459
BseDI CCNNGG 3 cut(s) 74, 297, 491
BseGI GGATG 1 cut(s) 89
BseMII CTCAG 1 cut(s) 148
BseNI ACTGG 2 cut(s) 404, 459
BseSI GKGCMC 1 cut(s) 233
BseXI GCAGC 1 cut(s) 524
Bsh1236I CGCG 1 cut(s) 197
BshFI GGCC 2 cut(s) 347, 476
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 1 cut(s) 233
BsiHKCI CYCGRG 1 cut(s) 236
BsiSI CCGG 1 cut(s) 545
BslFI GGGAC 1 cut(s) 509
BsmAI GTCTC 1 cut(s) 252
BsmFI GGGAC 1 cut(s) 509
BsmI GAATGC 2 cut(s) 358, 540
BsnI GGCC 2 cut(s) 347, 476
BsoBI CYCGRG 1 cut(s) 236
Bsp119I TTCGAA 1 cut(s) 129
Bsp1286I GDGCHC 1 cut(s) 233
Bsp143I GATC 1 cut(s) 279
BspACI CCGC 1 cut(s) 285
BspANI GGCC 2 cut(s) 347, 476
BspCNI CTCAG 1 cut(s) 147
BspFNI CGCG 1 cut(s) 197
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 1 cut(s) 287
BspQI GCTCTTC 2 cut(s) 306, 440
BspT104I TTCGAA 1 cut(s) 129
BspT107I GGYRCC 1 cut(s) 4
BsrI ACTGG 2 cut(s) 404, 459
BssECI CCNNGG 3 cut(s) 74, 297, 491
BssMI GATC 1 cut(s) 279
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 74, 491
Bst6I CTCTTC 4 cut(s) 219, 306, 336, 440
BstACI GRCGYC 1 cut(s) 5
BstBI TTCGAA 1 cut(s) 129
BstDEI CTNAG 1 cut(s) 134
BstF5I GGATG 1 cut(s) 89
BstFNI CGCG 1 cut(s) 197
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 1 cut(s) 282
BstMAI GTCTC 1 cut(s) 252
BstMBI GATC 1 cut(s) 279
BstMWI GCNNNNNNNGC 2 cut(s) 228, 353
BstNSI RCATGY 1 cut(s) 372
BstSLI GKGCMC 1 cut(s) 233
BstUI CGCG 1 cut(s) 197
BstV1I GCAGC 1 cut(s) 524
BstV2I GAAGAC 1 cut(s) 198
BstX2I RGATCY 1 cut(s) 279
BstYI RGATCY 1 cut(s) 279
BsuRI GGCC 2 cut(s) 347, 476
BtsCI GGATG 1 cut(s) 89
BtsI GCAGTG 1 cut(s) 309
BtsIMutI CAGTG 1 cut(s) 309
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 474
CseI GACGC 1 cut(s) 203
Csp6I GTAC 2 cut(s) 241, 265
CviAII CATG 3 cut(s) 11, 217, 369
CviQI GTAC 2 cut(s) 241, 265
DdeI CTNAG 1 cut(s) 134
DinI GGCGCC 1 cut(s) 6
DpnI GATC 1 cut(s) 281
DpnII GATC 1 cut(s) 279
Eam1104I CTCTTC 4 cut(s) 219, 306, 336, 440
EarI CTCTTC 4 cut(s) 219, 306, 336, 440
Eco130I CCWWGG 2 cut(s) 74, 491
Eco147I AGGCCT 1 cut(s) 347
Eco32I GATATC 2 cut(s) 121, 160
Eco57I CTGAAG 1 cut(s) 441
Eco88I CYCGRG 1 cut(s) 236
EcoRV GATATC 2 cut(s) 121, 160
EcoT14I CCWWGG 2 cut(s) 74, 491
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 74, 491
FaeI CATG 3 cut(s) 14, 220, 372
FaiI YATR 9 cut(s) 12, 113, 218, 245, 362, 370, 390, 428, 437
FaqI GGGAC 1 cut(s) 509
FatI CATG 3 cut(s) 10, 216, 368
FauI CCCGC 1 cut(s) 278
Fnu4HI GCNGC 1 cut(s) 538
FokI GGATG 1 cut(s) 76
Fsp4HI GCNGC 1 cut(s) 538
FspBI CTAG 2 cut(s) 492, 555
GlaI GCGC 1 cut(s) 6
GluI GCNGC 1 cut(s) 538
GsuI CTGGAG 1 cut(s) 338
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 2 cut(s) 347, 476
HapII CCGG 1 cut(s) 545
HgaI GACGC 1 cut(s) 203
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 14, 220, 372
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HpaII CCGG 1 cut(s) 545
Hpy166II GTNNAC 1 cut(s) 231
Hpy188I TCNGA 4 cut(s) 94, 118, 184, 528
Hpy8I GTNNAC 1 cut(s) 231
HpyAV CCTTC 2 cut(s) 452, 505
HpyCH4V TGCA 4 cut(s) 231, 356, 368, 540
HpyF10VI GCNNNNNNNGC 2 cut(s) 228, 353
HpyF3I CTNAG 1 cut(s) 134
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 14, 220, 372
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
Kzo9I GATC 1 cut(s) 279
LguI GCTCTTC 2 cut(s) 306, 440
LpnPI CCDG 6 cut(s) 61, 302, 379, 417, 440, 558
Lsp1109I GCAGC 1 cut(s) 524
MaeI CTAG 2 cut(s) 492, 555
MalI GATC 1 cut(s) 281
MboI GATC 1 cut(s) 279
MboII GAAGA 6 cut(s) 203, 236, 323, 353, 454, 457
MflI RGATCY 1 cut(s) 279
MhlI GDGCHC 1 cut(s) 233
MluCI AATT 2 cut(s) 31, 549
Mly113I GGCGCC 1 cut(s) 5
MnlI CCTC 9 cut(s) 18, 52, 148, 178, 192, 292, 323, 337, 465
MseI TTAA 2 cut(s) 102, 381
MslI CAYNNNNRTG 1 cut(s) 363
MspI CCGG 1 cut(s) 545
Mva1269I GAATGC 2 cut(s) 358, 540
MvnI CGCG 1 cut(s) 197
MwoI GCNNNNNNNGC 2 cut(s) 228, 353
NarI GGCGCC 1 cut(s) 5
NdeII GATC 1 cut(s) 279
NlaIII CATG 3 cut(s) 14, 220, 372
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 1 cut(s) 322
NspI RCATGY 1 cut(s) 372
NspV TTCGAA 1 cut(s) 129
OliI CACNNNNGTG 1 cut(s) 363
PceI AGGCCT 1 cut(s) 347
PciSI GCTCTTC 2 cut(s) 306, 440
PctI GAATGC 2 cut(s) 358, 540
PkrI GCNGC 1 cut(s) 539
PluTI GGCGCC 1 cut(s) 8
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 474
PsuI RGATCY 1 cut(s) 279
RsaI GTAC 2 cut(s) 242, 266
RsaNI GTAC 2 cut(s) 241, 265
RseI CAYNNNNRTG 1 cut(s) 363
SapI GCTCTTC 2 cut(s) 306, 440
SaqAI TTAA 2 cut(s) 102, 381
SatI GCNGC 1 cut(s) 538
Sau3AI GATC 1 cut(s) 279
Sau96I GGNCC 1 cut(s) 474
ScaI AGTACT 1 cut(s) 266
SduI GDGCHC 1 cut(s) 233
SfoI GGCGCC 1 cut(s) 6
SfuI TTCGAA 1 cut(s) 129
SmiMI CAYNNNNRTG 1 cut(s) 363
Sse9I AATT 2 cut(s) 31, 549
SseBI AGGCCT 1 cut(s) 347
SsiI CCGC 1 cut(s) 285
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 2 cut(s) 492, 555
StuI AGGCCT 1 cut(s) 347
StyI CCWWGG 2 cut(s) 74, 491
TaqI TCGA 3 cut(s) 129, 153, 533
TaqII GACCGA 1 cut(s) 264
TasI AATT 2 cut(s) 31, 549
TatI WGTACW 2 cut(s) 240, 264
Tru1I TTAA 2 cut(s) 102, 381
Tru9I TTAA 2 cut(s) 102, 381
TscAI CASTG 1 cut(s) 309
TseI GCWGC 1 cut(s) 537
TspDTI ATGAA 1 cut(s) 443
TspRI CASTG 1 cut(s) 309
VneI GTGCAC 1 cut(s) 229
XceI RCATGY 1 cut(s) 372
XmaJI CCTAGG 1 cut(s) 491
XspI CTAG 2 cut(s) 492, 555
ZrmI AGTACT 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.