Rroxscaffold_2G00105010

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0015897
Physical Location & Seq
Forward (+)
28446192 .. 28446764
573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00105010.1

Sequence Viewer

Length: 573 bp
ATGGCGCCTCATGGAGAAGCTATTGCTACCTCTTACTCTACATCCACCAAAAACATCTCCAAATCCAAACCACCAAGGCTCTCATTCTCATCCGATAGCCTTAAAAGAACCATATCGGATATTTCATTCGAACTCAGCAAGCAGAGCACTATCGAGGATCATAAAGCACTCCAACCCATTTCAGAGGTTGAAGACGCAAGGTGCGAGTGTTGTGGCATGAGCGAAGAGTGCACACCTGAGTACATAGACCGAGTACGAAACAAGTATTTGGGGAAGTGGATATGTGGGTTGTGTGCTGAGGCAGTGAAAGAAGAGCTGGAAAAAAATGGAGGGAACAAAGAAGAGGCCTTGAATGCACATATAAGTGCATGTGTTAGGTTTAACAAGTATAGTAGGGCTTATCCGGTTTTGTTCCAAGCTGAAGCTATGAAAGATATGTTGAAGAAGAGCAAATCAGAAGGGAGAAGTATGAGGGCTAAGTCCATCAGTCCTAGGGACAAAGGGGGATCAAGGAAGGGTGTGATTGCCCGGAGTTCGAGCTGTATTCCGGCAATTACTAGGGAGATGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

190

Amino Acids

21.06

Weight (kDa)

8.98

Isoelectric Point (pI)

66.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 63 - 152 2.2e-36 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015897)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79770
fragaria_vesca FvH4_6g32260
malus_domestica MD09G1205300.v1.1 MD17G1185200.v1.1
prunus_persica Prupe.3G060600_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0140141
rosa_laevigata RLG00000019855
rosa_multiflora Rmu_sc0003244.1_g000018
rosa_roxburghii Rroxscaffold_2G00105010
rosa_rugosa Rorug02G0361400
rosa_samantha Rh2AG412100 Rh2BG422500 Rh2CG398200 Rh2DG431800
rosa_wichuraiana Rw2G033770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AclWI GGATC 2 cut(s) 165, 514
AcuI CTGAAG 1 cut(s) 441
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 242, 255
AgsI TTSAA 3 cut(s) 191, 352, 442
AluBI AGCT 5 cut(s) 20, 316, 419, 425, 540
AluI AGCT 5 cut(s) 20, 316, 419, 425, 540
Alw21I GWGCWC 2 cut(s) 149, 233
Alw44I GTGCAC 1 cut(s) 229
AlwI GGATC 2 cut(s) 165, 514
AoxI GGCC 1 cut(s) 345
ApaLI GTGCAC 1 cut(s) 229
AspA2I CCTAGG 1 cut(s) 491
AspLEI GCGC 1 cut(s) 7
AsuC2I CCSGG 1 cut(s) 529
AsuII TTCGAA 1 cut(s) 129
AvrII CCTAGG 1 cut(s) 491
BaeGI GKGCMC 1 cut(s) 233
BanI GGYRCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 198
Bbv12I GWGCWC 2 cut(s) 149, 233
BbvCI CCTCAGC 1 cut(s) 297
BccI CCATC 1 cut(s) 491
BcnI CCSGG 1 cut(s) 529
BfaI CTAG 2 cut(s) 492, 558
BfoI RGCGCY 1 cut(s) 8
BlnI CCTAGG 1 cut(s) 491
Bme1390I CCNGG 1 cut(s) 529
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 529
BpiI GAAGAC 1 cut(s) 198
Bpu10I CCTNAGC 1 cut(s) 297
Bpu14I TTCGAA 1 cut(s) 129
BpuMI CCSGG 1 cut(s) 529
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 74, 491
BsaWI WCCGGW 1 cut(s) 403
BseDI CCNNGG 2 cut(s) 74, 491
BseGI GGATG 2 cut(s) 41, 89
BseMII CTCAG 3 cut(s) 148, 228, 288
BseSI GKGCMC 1 cut(s) 233
BshFI GGCC 1 cut(s) 347
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 2 cut(s) 149, 233
BsiSI CCGG 3 cut(s) 404, 529, 548
BslFI GGGAC 1 cut(s) 509
BsmFI GGGAC 1 cut(s) 509
BsmI GAATGC 1 cut(s) 358
BsnI GGCC 1 cut(s) 347
Bsp119I TTCGAA 1 cut(s) 129
Bsp1286I GDGCHC 2 cut(s) 149, 233
Bsp143I GATC 2 cut(s) 157, 506
BspANI GGCC 1 cut(s) 347
BspCNI CTCAG 3 cut(s) 147, 229, 289
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 2 cut(s) 165, 514
BspQI GCTCTTC 2 cut(s) 306, 440
BspT104I TTCGAA 1 cut(s) 129
BspT107I GGYRCC 1 cut(s) 4
BssECI CCNNGG 2 cut(s) 74, 491
BssMI GATC 2 cut(s) 157, 506
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 74, 491
Bst6I CTCTTC 4 cut(s) 219, 306, 336, 440
BstACI GRCGYC 1 cut(s) 5
BstBI TTCGAA 1 cut(s) 129
BstC8I GCNNGC 1 cut(s) 140
BstDEI CTNAG 4 cut(s) 134, 237, 297, 477
BstF5I GGATG 2 cut(s) 41, 89
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 2 cut(s) 160, 509
BstMBI GATC 2 cut(s) 157, 506
BstMWI GCNNNNNNNGC 3 cut(s) 144, 228, 353
BstNSI RCATGY 1 cut(s) 372
BstSCI CCNGG 1 cut(s) 527
BstSLI GKGCMC 1 cut(s) 233
BstV2I GAAGAC 1 cut(s) 198
BsuRI GGCC 1 cut(s) 347
BtsCI GGATG 2 cut(s) 41, 89
BtsI GCAGTG 1 cut(s) 309
BtsIMutI CAGTG 1 cut(s) 309
Cac8I GCNNGC 1 cut(s) 140
CfoI GCGC 1 cut(s) 7
CseI GACGC 1 cut(s) 203
Csp6I GTAC 2 cut(s) 241, 254
CviAII CATG 3 cut(s) 11, 217, 369
CviQI GTAC 2 cut(s) 241, 254
DdeI CTNAG 4 cut(s) 134, 237, 297, 477
DinI GGCGCC 1 cut(s) 6
DpnI GATC 2 cut(s) 159, 508
DpnII GATC 2 cut(s) 157, 506
Eam1104I CTCTTC 4 cut(s) 219, 306, 336, 440
EarI CTCTTC 4 cut(s) 219, 306, 336, 440
Eco130I CCWWGG 2 cut(s) 74, 491
Eco147I AGGCCT 1 cut(s) 347
Eco57I CTGAAG 1 cut(s) 441
EcoT14I CCWWGG 2 cut(s) 74, 491
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 74, 491
FaeI CATG 3 cut(s) 14, 220, 372
FaqI GGGAC 1 cut(s) 509
FatI CATG 3 cut(s) 10, 216, 368
FokI GGATG 2 cut(s) 28, 76
FspBI CTAG 2 cut(s) 492, 558
GlaI GCGC 1 cut(s) 6
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 347
HapII CCGG 3 cut(s) 404, 529, 548
HgaI GACGC 1 cut(s) 203
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 14, 220, 372
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HpaII CCGG 3 cut(s) 404, 529, 548
Hpy166II GTNNAC 1 cut(s) 231
Hpy188I TCNGA 4 cut(s) 94, 118, 184, 457
Hpy8I GTNNAC 1 cut(s) 231
HpyAV CCTTC 2 cut(s) 452, 508
HpyCH4V TGCA 3 cut(s) 231, 356, 368
HpyF10VI GCNNNNNNNGC 3 cut(s) 144, 228, 353
HpyF3I CTNAG 4 cut(s) 134, 237, 297, 477
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 14, 220, 372
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
Kzo9I GATC 2 cut(s) 157, 506
LguI GCTCTTC 2 cut(s) 306, 440
LpnPI CCDG 5 cut(s) 249, 302, 417, 542, 561
MaeI CTAG 2 cut(s) 492, 558
MalI GATC 2 cut(s) 159, 508
MboI GATC 2 cut(s) 157, 506
MboII GAAGA 6 cut(s) 203, 236, 323, 353, 454, 457
MhlI GDGCHC 2 cut(s) 149, 233
MluCI AATT 1 cut(s) 552
Mly113I GGCGCC 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 196
MnlI CCTC 8 cut(s) 18, 40, 148, 178, 292, 323, 337, 465
MseI TTAA 2 cut(s) 102, 381
MslI CAYNNNNRTG 1 cut(s) 363
MspI CCGG 3 cut(s) 404, 529, 548
MspR9I CCNGG 1 cut(s) 529
Mva1269I GAATGC 1 cut(s) 358
MwoI GCNNNNNNNGC 3 cut(s) 144, 228, 353
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 1 cut(s) 529
NdeII GATC 2 cut(s) 157, 506
NlaIII CATG 3 cut(s) 14, 220, 372
NlaIV GGNNCC 1 cut(s) 6
NspI RCATGY 1 cut(s) 372
NspV TTCGAA 1 cut(s) 129
PceI AGGCCT 1 cut(s) 347
PciSI GCTCTTC 2 cut(s) 306, 440
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PctI GAATGC 1 cut(s) 358
PluTI GGCGCC 1 cut(s) 8
PspN4I GGNNCC 1 cut(s) 6
RsaI GTAC 2 cut(s) 242, 255
RsaNI GTAC 2 cut(s) 241, 254
RseI CAYNNNNRTG 1 cut(s) 363
SapI GCTCTTC 2 cut(s) 306, 440
SaqAI TTAA 2 cut(s) 102, 381
Sau3AI GATC 2 cut(s) 157, 506
ScrFI CCNGG 1 cut(s) 529
SduI GDGCHC 2 cut(s) 149, 233
SfoI GGCGCC 1 cut(s) 6
SfuI TTCGAA 1 cut(s) 129
SmiMI CAYNNNNRTG 1 cut(s) 363
Sse9I AATT 1 cut(s) 552
SseBI AGGCCT 1 cut(s) 347
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 2 cut(s) 492, 558
StuI AGGCCT 1 cut(s) 347
StyD4I CCNGG 1 cut(s) 527
StyI CCWWGG 2 cut(s) 74, 491
TaqI TCGA 3 cut(s) 129, 153, 536
TaqII GACCGA 1 cut(s) 264
TasI AATT 1 cut(s) 552
TatI WGTACW 1 cut(s) 240
Tru1I TTAA 2 cut(s) 102, 381
Tru9I TTAA 2 cut(s) 102, 381
TscAI CASTG 1 cut(s) 309
TspDTI ATGAA 2 cut(s) 114, 443
TspRI CASTG 1 cut(s) 309
VneI GTGCAC 1 cut(s) 229
XceI RCATGY 1 cut(s) 372
XmaJI CCTAGG 1 cut(s) 491
XspI CTAG 2 cut(s) 492, 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.