MD17G1185200.v1.1

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0015897
Physical Location & Seq
Forward (+)
21994660 .. 21995238
579 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1185200.v1.1.491

Sequence Viewer

Length: 579 bp
ATGGCACCTCCTGGAGAGTTTATTTCTGGCTCTAGGGCCAACAACATCTCCAAGTGTAAGCCACCAACGCTTTCATCCTCAGCAGATAGCCTTAAAAGAACAATATCCGACATCTCTTACGAGCTCATCAAAGAAGTCAAAATTGATCAGGATCTGAAATCCCTTCCGCCAATATCTGAGGTGGAAGATGCAAAGTGTGAATGTTGTGGCATGAGTGAAGAGTGCACGCCCGAGTACATAGACCGAGTGAGAGACAAGTTCTTGGGGAAGTGGATATGCGGGTTGTGTTCTGAGGCAGTGAAAGAAGAGGTTGAGAAAAATGGAGGAAATAAAGAAGAGGCTTTGAATTCACATATAAGTGCATGTGTTAGGTTTAACAAGTATAGTAGGGCGTATCCGGTTTTGTTTCAAGCTGAGGCTATGAGGGAGATGTTCAAGAAGAGTAAGTCAGACGGGACGGGCATGAGGGCTAAGTCAATTAGTCCGAAAGACAAAGGAGGGCCAAAGGGGAAGATTGCTCGGAGTTCAAGCTGCATTCCAGCGATTACGAGGGAGATGAACGATCTCTCCATGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

193

Amino Acids

21.11

Weight (kDa)

8.32

Isoelectric Point (pI)

52.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 61 - 150 7.7e-37 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015897)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79770
fragaria_vesca FvH4_6g32260
malus_domestica MD09G1205300.v1.1 MD17G1185200.v1.1
prunus_persica Prupe.3G060600_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0140141
rosa_laevigata RLG00000019855
rosa_multiflora Rmu_sc0003244.1_g000018
rosa_roxburghii Rroxscaffold_2G00105010
rosa_rugosa Rorug02G0361400
rosa_samantha Rh2AG412100 Rh2BG422500 Rh2CG398200 Rh2DG431800
rosa_wichuraiana Rw2G033770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AciI CCGC 2 cut(s) 167, 279
AclWI GGATC 1 cut(s) 159
AcsI RAATTY 1 cut(s) 346
AfaI GTAC 1 cut(s) 236
AgsI TTSAA 4 cut(s) 346, 410, 436, 528
AjnI CCWGG 1 cut(s) 10
AloI GAACNNNNNNTCC 1 cut(s) 551
AluBI AGCT 3 cut(s) 124, 413, 531
AluI AGCT 3 cut(s) 124, 413, 531
Alw21I GWGCWC 2 cut(s) 126, 227
Alw26I GTCTC 1 cut(s) 246
Alw44I GTGCAC 1 cut(s) 223
AlwI GGATC 1 cut(s) 159
AlwNI CAGNNNCTG 1 cut(s) 154
Ama87I CYCGRG 1 cut(s) 230
AoxI GGCC 2 cut(s) 36, 500
ApaLI GTGCAC 1 cut(s) 223
ApeKI GCWGC 1 cut(s) 531
ApoI RAATTY 1 cut(s) 346
AspS9I GGNCC 2 cut(s) 36, 500
AvaI CYCGRG 1 cut(s) 230
BaeGI GKGCMC 1 cut(s) 227
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 126
Bbv12I GWGCWC 2 cut(s) 126, 227
BbvCI CCTCAGC 2 cut(s) 79, 414
BbvI GCAGC 1 cut(s) 518
BciT130I CCWGG 1 cut(s) 12
BciVI GTATCC 1 cut(s) 405
BclI TGATCA 1 cut(s) 145
BcoDI GTCTC 1 cut(s) 246
BfaI CTAG 1 cut(s) 33
BfuI GTATCC 1 cut(s) 405
BisI GCNGC 1 cut(s) 532
BlsI GCNGC 1 cut(s) 533
Bme1390I CCNGG 1 cut(s) 12
BmeT110I CYCGRG 1 cut(s) 230
BmgT120I GGNCC 2 cut(s) 36, 500
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 12
BmsI GCATC 1 cut(s) 178
BpmI CTGGAG 1 cut(s) 33
Bpu10I CCTNAGC 2 cut(s) 79, 414
BsaBI GATNNNNATC 1 cut(s) 150
BsaJI CCNNGG 1 cut(s) 570
BsaWI WCCGGW 1 cut(s) 397
BsaXI ACNNNNNCTCC 3 cut(s) 32, 62, 551
Bse8I GATNNNNATC 1 cut(s) 150
BseBI CCWGG 1 cut(s) 12
BseDI CCNNGG 1 cut(s) 570
BseGI GGATG 1 cut(s) 74
BseJI GATNNNNATC 1 cut(s) 150
BseMII CTCAG 4 cut(s) 93, 168, 282, 405
BseSI GKGCMC 1 cut(s) 227
BseXI GCAGC 1 cut(s) 518
BshFI GGCC 2 cut(s) 38, 502
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 2 cut(s) 126, 227
BsiHKCI CYCGRG 1 cut(s) 230
BsiSI CCGG 1 cut(s) 398
BslFI GGGAC 1 cut(s) 469
BsmAI GTCTC 1 cut(s) 246
BsmFI GGGAC 1 cut(s) 469
BsmI GAATGC 1 cut(s) 534
BsnI GGCC 2 cut(s) 38, 502
BsoBI CYCGRG 1 cut(s) 230
Bsp1286I GDGCHC 2 cut(s) 126, 227
Bsp143I GATC 3 cut(s) 145, 151, 562
Bsp19I CCATGG 1 cut(s) 570
BspACI CCGC 2 cut(s) 167, 279
BspANI GGCC 2 cut(s) 38, 502
BspCNI CTCAG 4 cut(s) 92, 169, 283, 406
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 1 cut(s) 159
BspT107I GGYRCC 1 cut(s) 4
BssECI CCNNGG 1 cut(s) 570
BssMI GATC 3 cut(s) 145, 151, 562
BssT1I CCWWGG 1 cut(s) 570
Bst2UI CCWGG 1 cut(s) 12
Bst6I CTCTTC 4 cut(s) 213, 300, 330, 434
BstC8I GCNNGC 1 cut(s) 227
BstDEI CTNAG 5 cut(s) 79, 177, 291, 414, 471
BstDSI CCRYGG 1 cut(s) 570
BstF5I GGATG 1 cut(s) 74
BstKTI GATC 3 cut(s) 148, 154, 565
BstMAI GTCTC 1 cut(s) 246
BstMBI GATC 3 cut(s) 145, 151, 562
BstMWI GCNNNNNNNGC 1 cut(s) 67
BstNI CCWGG 1 cut(s) 12
BstNSI RCATGY 1 cut(s) 366
BstSCI CCNGG 1 cut(s) 10
BstSLI GKGCMC 1 cut(s) 227
BstV1I GCAGC 1 cut(s) 518
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
BsuI GTATCC 1 cut(s) 405
BsuRI GGCC 2 cut(s) 38, 502
BtgI CCRYGG 1 cut(s) 570
BtsCI GGATG 1 cut(s) 74
BtsI GCAGTG 1 cut(s) 303
BtsIMutI CAGTG 1 cut(s) 303
Cac8I GCNNGC 1 cut(s) 227
CaiI CAGNNNCTG 1 cut(s) 154
Cfr13I GGNCC 2 cut(s) 36, 500
Csp6I GTAC 1 cut(s) 235
CviAII CATG 4 cut(s) 211, 363, 463, 571
CviQI GTAC 1 cut(s) 235
DdeI CTNAG 5 cut(s) 79, 177, 291, 414, 471
DpnI GATC 3 cut(s) 147, 153, 564
DpnII GATC 3 cut(s) 145, 151, 562
Eam1104I CTCTTC 4 cut(s) 213, 300, 330, 434
EarI CTCTTC 4 cut(s) 213, 300, 330, 434
EciI GGCGGA 1 cut(s) 156
Ecl136II GAGCTC 1 cut(s) 124
Eco130I CCWWGG 1 cut(s) 570
Eco24I GRGCYC 1 cut(s) 126
Eco53kI GAGCTC 1 cut(s) 124
Eco88I CYCGRG 1 cut(s) 230
EcoICRI GAGCTC 1 cut(s) 124
EcoRI GAATTC 1 cut(s) 346
EcoRII CCWGG 1 cut(s) 10
EcoT14I CCWWGG 1 cut(s) 570
EcoT38I GRGCYC 1 cut(s) 126
ErhI CCWWGG 1 cut(s) 570
FaeI CATG 4 cut(s) 214, 366, 466, 574
FaqI GGGAC 1 cut(s) 469
FatI CATG 4 cut(s) 210, 362, 462, 570
FauI CCCGC 1 cut(s) 272
FbaI TGATCA 1 cut(s) 145
Fnu4HI GCNGC 1 cut(s) 532
FokI GGATG 1 cut(s) 61
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 1 cut(s) 532
FspBI CTAG 1 cut(s) 33
GluI GCNGC 1 cut(s) 532
GsuI CTGGAG 1 cut(s) 33
HaeIII GGCC 2 cut(s) 38, 502
HapII CCGG 1 cut(s) 398
Hin1II CATG 4 cut(s) 214, 366, 466, 574
HpaII CCGG 1 cut(s) 398
Hpy166II GTNNAC 1 cut(s) 225
Hpy188I TCNGA 7 cut(s) 109, 156, 178, 292, 451, 486, 522
Hpy188III TCNNGA 2 cut(s) 149, 436
Hpy8I GTNNAC 1 cut(s) 225
HpyAV CCTTC 1 cut(s) 173
HpyCH4V TGCA 4 cut(s) 191, 225, 362, 534
HpyF10VI GCNNNNNNNGC 1 cut(s) 67
HpyF3I CTNAG 5 cut(s) 79, 177, 291, 414, 471
Hsp92II CATG 4 cut(s) 214, 366, 466, 574
Ksp22I TGATCA 1 cut(s) 145
Kzo9I GATC 3 cut(s) 145, 151, 562
LpnPI CCDG 5 cut(s) 12, 24, 134, 411, 552
Lsp1109I GCAGC 1 cut(s) 518
LweI GCATC 1 cut(s) 178
MaeI CTAG 1 cut(s) 33
MalI GATC 3 cut(s) 147, 153, 564
MboI GATC 3 cut(s) 145, 151, 562
MboII GAAGA 6 cut(s) 197, 230, 317, 347, 451, 523
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 2 cut(s) 126, 227
MluCI AATT 3 cut(s) 141, 346, 477
MmeI TCCRAC 1 cut(s) 132
MseI TTAA 2 cut(s) 93, 375
MslI CAYNNNNRTG 1 cut(s) 357
MspI CCGG 1 cut(s) 398
MspR9I CCNGG 1 cut(s) 12
Mva1269I GAATGC 1 cut(s) 534
MvaI CCWGG 1 cut(s) 12
MwoI GCNNNNNNNGC 1 cut(s) 67
NcoI CCATGG 1 cut(s) 570
NdeII GATC 3 cut(s) 145, 151, 562
NlaIII CATG 4 cut(s) 214, 366, 466, 574
NlaIV GGNNCC 1 cut(s) 6
NspI RCATGY 1 cut(s) 366
PctI GAATGC 1 cut(s) 534
PfoI TCCNGGA 1 cut(s) 10
PkrI GCNGC 1 cut(s) 533
Psp124BI GAGCTC 1 cut(s) 126
Psp6I CCWGG 1 cut(s) 10
PspGI CCWGG 1 cut(s) 10
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 2 cut(s) 36, 500
PstNI CAGNNNCTG 1 cut(s) 154
PsuI RGATCY 1 cut(s) 151
RsaI GTAC 1 cut(s) 236
RsaNI GTAC 1 cut(s) 235
RseI CAYNNNNRTG 1 cut(s) 357
SacI GAGCTC 1 cut(s) 126
SaqAI TTAA 2 cut(s) 93, 375
SatI GCNGC 1 cut(s) 532
Sau3AI GATC 3 cut(s) 145, 151, 562
Sau96I GGNCC 2 cut(s) 36, 500
ScrFI CCNGG 1 cut(s) 12
SduI GDGCHC 2 cut(s) 126, 227
SetI ASST 7 cut(s) 10, 126, 183, 312, 374, 415, 533
SfaNI GCATC 1 cut(s) 178
SmiMI CAYNNNNRTG 1 cut(s) 357
Sse9I AATT 3 cut(s) 141, 346, 477
SsiI CCGC 2 cut(s) 167, 279
SspMI CTAG 1 cut(s) 33
SstI GAGCTC 1 cut(s) 126
StyD4I CCNGG 1 cut(s) 10
StyI CCWWGG 1 cut(s) 570
TaqII GACCGA 1 cut(s) 258
TasI AATT 3 cut(s) 141, 346, 477
TatI WGTACW 1 cut(s) 234
Tru1I TTAA 2 cut(s) 93, 375
Tru9I TTAA 2 cut(s) 93, 375
TscAI CASTG 1 cut(s) 303
TseI GCWGC 1 cut(s) 531
TspDTI ATGAA 2 cut(s) 63, 572
TspRI CASTG 1 cut(s) 303
VneI GTGCAC 1 cut(s) 223
XapI RAATTY 1 cut(s) 346
XceI RCATGY 1 cut(s) 366
XspI CTAG 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.